HG10015701 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015701 vs. NCBI nr
Match: XP_038892703.1 (crossover junction endonuclease MUS81 isoform X1 [Benincasa hispida])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 580/649 (89.37%), Postives = 598/649 (92.14%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPVVCPENED+A FMWQKRLEMA TPKGISDKVDMTLYKAYFN+CNSK PIKSMK
Sbjct: 1   MDRQRSPVVCPENEDIATFMWQKRLEMAATPKGISDKVDMTLYKAYFNVCNSKVPIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+ DFFK+GSEGSGPEEL GKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVHDFFKNGSEGSGPEELVGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDA+EASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDASEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK SQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLS
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           STKN DNTKGSSILESCDISDHSAN LDSDVEVTSP+  KSSQNKS DVPLESLERF+RM
Sbjct: 241 STKNVDNTKGSSILESCDISDHSANSLDSDVEVTSPTAIKSSQNKSTDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENR-SMGSSSHM 360
           GYSK+QVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENR +  SSSH 
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTSSSSSHD 360

Query: 361 GNGPKIVVNDDGKRSFNLRACSSSNPSI-TESRRDGVKDNMNILSMPPLSFGEKFEDSYN 420
            NGPKIVVND+G+R FNLRACSSSNPSI +ES RDGVK NMNILSMPPLSFGEKFEDSYN
Sbjct: 361 RNGPKIVVNDNGERFFNLRACSSSNPSISSESCRDGVKANMNILSMPPLSFGEKFEDSYN 420

Query: 421 VVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480
           VVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF
Sbjct: 421 VVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDF 480

Query: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEI 540
           IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEI
Sbjct: 481 IVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEI 540

Query: 541 LEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDK 600
           LEGFDVQRTSGLSDTLKKYGYLTRAITQYY+LQASDERLSHSD CPRLNEFIKKCQELDK
Sbjct: 541 LEGFDVQRTSGLSDTLKKYGYLTRAITQYYILQASDERLSHSDVCPRLNEFIKKCQELDK 600

Query: 601 MTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDICDR 648
           MTVSDVFALQLMQVPQVTEEVAVAVL+LYPTLLSLARAYSLL  ++ ++
Sbjct: 601 MTVSDVFALQLMQVPQVTEEVAVAVLELYPTLLSLARAYSLLDGNVTEQ 624

BLAST of HG10015701 vs. NCBI nr
Match: ADN34204.1 (endonuclease [Cucumis melo subsp. melo])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 568/644 (88.20%), Postives = 595/644 (92.39%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK SQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLS
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S+KNFDN KGSSILESCDIS+HSANYLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QV++SFKEVS+DNPNKD SSLWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEG 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PK VVND+G+RSFN RACSSSNPSI ESRRD VK++ NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFA HSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERK+IDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVS I
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSSI 617

BLAST of HG10015701 vs. NCBI nr
Match: XP_008446914.1 (PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo] >XP_008446915.1 PREDICTED: crossover junction endonuclease MUS81 isoform X1 [Cucumis melo])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 565/647 (87.33%), Postives = 596/647 (92.12%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK SQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLS
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S+KNFDN KGSSILESCDIS+HSANYLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QV++SFKEVS+DNPNKD SSLWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEG 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PK VVND+G+RSFN RACSSSNPSI ESRRD VK++ NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFA HSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERK+IDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDICDR 648
           VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLL  ++ ++
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQ 620

BLAST of HG10015701 vs. NCBI nr
Match: XP_023514312.1 (crossover junction endonuclease MUS81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 563/644 (87.42%), Postives = 591/644 (91.77%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q FFK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK  QFGSS RDWYSGW+CMKTL+SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S KNFD  KGSSILESCDISD SAN+LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RM
Sbjct: 241 SEKNFDTMKGSSILESCDISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRS GS+   G
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSNE--G 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PKIVV+D+G+RSFNLRACSSSN SITESR+D VK+N NILSMPPLSFGEKFED+Y++V
Sbjct: 361 HVPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSIV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASDERLSH D CPRL+ FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLL  ++
Sbjct: 601 VSDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNV 617

BLAST of HG10015701 vs. NCBI nr
Match: XP_022956424.1 (crossover junction endonuclease MUS81 [Cucurbita moschata])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 562/644 (87.27%), Postives = 590/644 (91.61%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q  FK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK  QFGSS RDWYSGW+CMKTL+SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S KNFD  KGSSILESC ISD SAN+LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRS GSS+  G
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNE-G 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PKIVV+D+G+RSFNLRACSSSN SITESR+D VK+N NILSMPPLSFGEKFED+Y+VV
Sbjct: 361 HVPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFANHSSRSRRMIENICSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASDERLSH D CPRL+ FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLL  ++
Sbjct: 601 VSDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNV 618

BLAST of HG10015701 vs. ExPASy Swiss-Prot
Match: Q5W9E7 (Crossover junction endonuclease MUS81 OS=Arabidopsis thaliana OX=3702 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 7.6e-179
Identity = 345/664 (51.96%), Postives = 448/664 (67.47%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E AE PKG+S+ ++ T  K Y ++C++K PI ++K
Sbjct: 1   MDDERR-VLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGK--GKKTKGTKRYVPQKNSVAYALLI 120
           DLSQIKG GKW++K ++ +F +  E S  E+L     GKK  G KRY+PQ+NSV YALLI
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPE 180
           TL+                         R TTNG E+MRKQELIDAA+A+GLS   +GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180

Query: 181 KGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGL 240
           KGKGKA   G S R+WYSGWSCM TLI +GLVVKS+ PAKYMLT EG+ VA EC++RSGL
Sbjct: 181 KGKGKAG-LGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240

Query: 241 LSSTK--NFDNTKGSSILESCDISDHSANY-LDSDVEVTSPSLSKSSQNKSIDVPLESLE 300
             S    + D    +   +     + + ++ +  ++    P     S      +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDPRCRAQSA-----IPSDILE 300

Query: 301 RFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGS 360
           +F   GYSK QV+ +F+EVS+ + +KD S+LW +V+C LR+  VY    +      S   
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVY---NSCPDSRNSKKD 360

Query: 361 SSHMGNGPKIVVNDDGKRSFNLRAC---SSSNPSITESRR-----------DGVKDNMNI 420
           SS         V+ +G R+   R+C   S+ NP  + S             DG K   NI
Sbjct: 361 SSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITNI 420

Query: 421 LSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDG 480
             +PPL FGE FE++Y+V+LILDDRE+FA   SRSR ++ENICS+F I+IE+RRLPVGD 
Sbjct: 421 PRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGDC 480

Query: 481 IWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPN 540
           IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G K++IY++EGDPN
Sbjct: 481 IWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDPN 540

Query: 541 SSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSD 600
            S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY L+ +D   S   
Sbjct: 541 HSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKGA 600

Query: 601 A-CPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLL 645
           A CP  + F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS L
Sbjct: 601 ASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSHL 629

BLAST of HG10015701 vs. ExPASy Swiss-Prot
Match: Q8GT06 (Crossover junction endonuclease MUS81 OS=Oryza sativa subsp. japonica OX=39947 GN=MUS81 PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 4.1e-140
Identity = 306/659 (46.43%), Postives = 400/659 (60.70%), Query Frame = 0

Query: 12  ENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKW 71
           ENE +A  + +K   M E P G+ + +  TLYK+Y N+C +K PI+S+KDL QIKGVGKW
Sbjct: 14  ENEAVAQCVLEKWRSMEEKPGGLKENLAHTLYKSYRNVCAAKEPIRSLKDLYQIKGVGKW 73

Query: 72  ILKQIQDFFKSGSEGSGPEE--LAG-KGKKTKGTKRYVPQKNSVAYALLITLYRYNGQIV 131
           +++Q++  F   S    P E   AG KGKK  G+KRYVPQKNS AYA+LITL+       
Sbjct: 74  VIRQLKGSFPESSPDLSPPESNAAGEKGKKAGGSKRYVPQKNSAAYAILITLH------- 133

Query: 132 ISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFG 191
                             R T NG  +M+KQELIDA EASGLSR AIGP+K K K   F 
Sbjct: 134 ------------------RETINGKSHMKKQELIDATEASGLSRSAIGPDKSKAKPGAFA 193

Query: 192 SSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNT 251
           SS +DWY+GWSCMKTL S+GLV KS  PAKYM+TEEG+  A ECL RSGL       D+ 
Sbjct: 194 SSQKDWYTGWSCMKTLTSKGLVAKSGNPAKYMITEEGKSTALECLSRSGL-------DDH 253

Query: 252 KGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVL 311
               ++ S   + ++++ L +++ +TS   + S  +++I  P  S+         +   L
Sbjct: 254 AAPLVINSAPDTSNASHKL-NNICMTSFVETSSGPSRAIGRPKTSIANPATKTSPEVTYL 313

Query: 312 DSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMGN---GPKI 371
            S + +   N N D+ +   A  C     L     E +  EN  +  S         P +
Sbjct: 314 TSQESL---NYNSDVRT---AENCAEEIILSDSDSEELYTENYPLIGSEEFTERVAPPIL 373

Query: 372 VVNDDGKRSFNLR--ACSSS-NPSITESR------------------RDGVKDNMNILSM 431
             ++ GK + N R   CS+S +P  +E                     D V  + +IL+M
Sbjct: 374 NASNSGKTTTNYRFSDCSASISPRSSEGTFEMQSSSTMGIAEFNMLDNDTVCMDNSILAM 433

Query: 432 PPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWI 491
           PP    + F + Y VVLILDDRE F     RSR+ ++NI SQF++ +EI+ LPVGDGIWI
Sbjct: 434 PPRRSSKNFLEDYEVVLILDDRENF---GGRSRKTVDNIHSQFRVPVEIKHLPVGDGIWI 493

Query: 492 ARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSE 551
           AR + L  EYVLDFIVERKN+ DL  SI DNRY+DQKL+L +CGL+++IYLVEGDPN  +
Sbjct: 494 ARDRKLHTEYVLDFIVERKNVADLCSSITDNRYKDQKLRLKKCGLRKLIYLVEGDPNPLD 553

Query: 552 AAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACP 611
            +E IKTACFTTEILEGFDVQRT G ++T++ YG LT +IT+YY    S    + S  C 
Sbjct: 554 TSERIKTACFTTEILEGFDVQRTPGYAETVRTYGNLTHSITEYYSTHFS-TGANTSQVCL 613

Query: 612 RLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSD 644
             +EF KKC +L K+TVSDVFALQLMQVPQVTEE A+AV+ LYPTL SLA+AYS+L  D
Sbjct: 614 TYDEFTKKCDDLKKITVSDVFALQLMQVPQVTEEAALAVIGLYPTLFSLAKAYSMLDGD 629

BLAST of HG10015701 vs. ExPASy Swiss-Prot
Match: Q6BJ48 (Crossover junction endonuclease MUS81 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=MUS81 PE=3 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-29
Identity = 170/686 (24.78%), Postives = 277/686 (40.38%), Query Frame = 0

Query: 21  WQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGK----WILKQI 80
           W +   +  T KG   K  +   KA  ++ N   PI   K L  ++ VG      + K++
Sbjct: 15  WLQEAAINATKKG--TKAAILYNKALGSVRNYPLPINDPKTLKSVQFVGDKTCIHLSKKL 74

Query: 81  QDFFKSGS---------------------EGSGPEELA-GKGKKTKGTKRYVPQKNSVAY 140
           +++ K  +                     E S    L   K KK +  K+Y+P+K S  Y
Sbjct: 75  EEYCKLNNFELPVAFGGLINGGVGEKRKHEVSDTSNLPDAKPKKQRKQKQYIPRKRSGGY 134

Query: 141 ALLITLYRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVA 200
           A+L+ LY  + +                             + K+E+I  A         
Sbjct: 135 AILLALYFGDKK--------------------------KTGLTKEEIIQRATPY------ 194

Query: 201 IGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAK-YMLTEEGQHVARECL 260
                   K+ +   S  ++YS WS +K+L +  LV  S   +K Y LTEEG  +A++  
Sbjct: 195 ------SDKSFKSNPSANEFYSAWSSIKSLQTHDLVDSSGRSSKSYFLTEEGYELAKQ-- 254

Query: 261 MRSGLLSSTKNFDNTKGSSILESCDISDHSANY-LDSDVEVTSPSLSKSSQNKSIDVPLE 320
                          K +   ES  I++H A+   D+ V VT  S               
Sbjct: 255 --------------LKDAEGFESSPITNHIADLSFDNQVRVTPDS--------------- 314

Query: 321 SLERFLRMGYSK-NQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENR 380
                    YSK +Q LDS   +   N NKD   L P+ L   RE ++            
Sbjct: 315 --------SYSKISQQLDSSPLMKTKN-NKDRYGLGPSRLSSTRERIL------------ 374

Query: 381 SMGSSSHMGNGPKIVVNDDGKRSFNLR-ACSSSNPSITESRRDGV---KDNMNILSMPPL 440
                  + + P+++ +    + F LR + SS N +     R+ +   +D +N  +   L
Sbjct: 375 ------DLSSSPRLISSPLKPKDFVLRESLSSHNSTRPLQDRESISKAEDGINTKTKARL 434

Query: 441 SF--------GEKFE----DSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRR 500
                     G  ++      + ++LI+D+RE     S R R   +   +  K++ ++R 
Sbjct: 435 VHDASKRIYDGTNYDIWVPGEFEIILIIDNRE---IRSQRDRDFFQTRLTSLKVECDVRP 494

Query: 501 LPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYL 560
           L VGD +W A+HK    E +L++I ERK +DDL  SI+D R+++QK +L + G+K+  YL
Sbjct: 495 LSVGDVVWTAKHKKTGREVILNYICERKRLDDLVSSIKDGRFQEQKNRLKKSGMKQFYYL 554

Query: 561 VE----GDPNS-SEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAI------ 620
           VE     D N   + +++I+TA   T  +  F ++R   + DT+     LT+ I      
Sbjct: 555 VEDVVTSDMNKFGDMSDAIQTAMSMTMTISNFYLKRFKSIEDTIAFLASLTQVIKDQFAK 599

Query: 621 --TQYYVLQASD------------------ERLSHSDACPRL-NEFIKKCQELDKMTVSD 630
             T   VL+A                    E  S S  C  L + F     +   MTV +
Sbjct: 615 NKTNLLVLKARSIKNQAEYSSLIAKFKEKFENRSTSYECAHLFSTFQDSMGKTGMMTVKE 599

BLAST of HG10015701 vs. ExPASy Swiss-Prot
Match: Q6CPQ8 (Crossover junction endonuclease MUS81 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=MUS81 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-29
Identity = 154/672 (22.92%), Postives = 261/672 (38.84%), Query Frame = 0

Query: 26  EMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKWILKQIQDFFKSGSE 85
           E+ ++     +K+ M L +A F + N   PI   KDL ++KG+G+  + ++    K+  E
Sbjct: 18  EVNQSTSAHQEKLVMVLNRALFALKNYPDPIYHPKDLLKVKGIGQTTMNKLSKRLKTYCE 77

Query: 86  GSG----------------------------------PEELAGKGKKTKGTKRYVPQKNS 145
            +G                                  PEE   +G + +  K+Y+P+  S
Sbjct: 78  ENGYSFPEESSSTDNTQSTQNDSNPSNTQKTRVRTLEPEENESQGTRKRRKKKYIPRNRS 137

Query: 146 VAYALLITLYRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLS 205
             Y +L+                L +  C+         +G    R+Q +  A+      
Sbjct: 138 GGYGILLG---------------LLELGCD--------KDGTACTRRQLIAVAS------ 197

Query: 206 RVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARE 265
                  K   ++ +   S +++YS WS +K+L +  LV++   P+++ LTE G  +A  
Sbjct: 198 -------KYCDQSYEKNPSTKEFYSAWSAIKSLKTNDLVIEQGRPSQFSLTEAGTILA-- 257

Query: 266 CLMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPL 325
                         D+ K             +AN ++ DV                    
Sbjct: 258 --------------DSLK-------------TANNIEFDVS------------------- 317

Query: 326 ESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENR 385
              ER L  G ++N   +      E +   + S L       + E             N 
Sbjct: 318 SVYERRLNRGNTQNSFTN-----DEHDHTVNFSGLMNHANMSINES-----------ANS 377

Query: 386 SMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGE 445
           S        N  +I  N++ + S  + +       +++ R  GVK  +      P     
Sbjct: 378 SRLFLDATANSSRIEQNEEPEVSAEI-STPIPKQKVSKGRWKGVKYEL----WKP----- 437

Query: 446 KFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLD 505
              DSY+++L +D RE     S   R        Q  I+ E   L VGD IW+A+HK   
Sbjct: 438 ---DSYDIILHIDHREV---RSKEDRGFFARKLLQRGIETESSSLTVGDMIWLAKHKQSG 497

Query: 506 NEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDP--NSSEAAESI 565
            +  LDFIVERK +DDL  SIRDNR+ +QK +L + G K + YLVE     N S++A+ +
Sbjct: 498 QQCALDFIVERKRLDDLVISIRDNRFSEQKNRLQKTGCKHIFYLVEETTGYNVSDSADMM 557

Query: 566 KTACFTTEILEGFDVQRTSGLSDTLK---------------------KYGYLTRA---IT 625
           KT+ +TT I   F ++RT     T++                      + ++T     +T
Sbjct: 558 KTSIWTTVIYNDFHIKRTRNADTTVQWLTDMSLIIKELYSRKSLVVINHDHITNQSIYLT 573

Query: 626 QYYVLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLD 638
              + +   ER    + C           + + MTV +++   LM V  ++ E A+ +  
Sbjct: 618 SLKMFRTEFERNKEIECCHNYESMQSAMVKTNLMTVKELYLRALMSVKGISLEKALMIQS 573

BLAST of HG10015701 vs. ExPASy Swiss-Prot
Match: Q7SXA9 (Crossover junction endonuclease MUS81 OS=Danio rerio OX=7955 GN=mus81 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 9.4e-28
Identity = 177/708 (25.00%), Postives = 279/708 (39.41%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           + R+R    CP    L    W   L  +   KG+  K      KA  ++     P+K+ K
Sbjct: 8   LGRKRPLPSCPNPLFL---QWLTELRDSAKEKGL--KTHFVYQKAINSLKKYPLPLKNGK 67

Query: 61  DLSQIKGVGKWILKQIQD-----FFKSGSEGS-----------GPEELAG---------- 120
           +   ++  G  I K + +     + ++GS+ +             +E +G          
Sbjct: 68  EAKILQNFGDGICKILDERLQKHYRENGSDAAVHLASSKQMEESQKEPSGNFSEHTKLTQ 127

Query: 121 -KGKKTKGTKR---YVPQKNSVAYALLITLYRYNGQIVISFVKFLFKFTCNIRSTIRGTT 180
            + +K KG K+   YVPQK S  YA+L+TLYR+                           
Sbjct: 128 KEVRKEKGAKKKREYVPQKRSGGYAVLLTLYRH-----------------------MQMP 187

Query: 181 NGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRGLV 240
               +M + EL    EA  L   +             GS     Y+ WS + TLI + LV
Sbjct: 188 GSKGFMFRNEL--QTEAQPLCEKSF-------TVPDLGSK----YTAWSSVSTLIQKELV 247

Query: 241 VKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLDSD 300
           VK++ PA+Y LT++                         G S+ E  D  +    + D D
Sbjct: 248 VKTHNPARYSLTDQ-------------------------GLSLAEKLDSEETGTRHEDVD 307

Query: 301 VEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAV 360
                   S+  QN  +D+ LE                    E  ED   +  SS  PAV
Sbjct: 308 --------SQDGQN-VVDLTLE--------------------EEDEDEEKESWSSERPAV 367

Query: 361 LCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSITES 420
                  L   Q   +  E+  MG S           ++ G+ +                
Sbjct: 368 A------LPVNQARGLMSESDPMGKSQ---------TSETGRTAMGWH------------ 427

Query: 421 RRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQ 480
                           LS G     SY++VL +D  E     S R + +++ +  +  + 
Sbjct: 428 ----------------LSPG-----SYDIVLCVDLCETTGGSSVRKQELVKEL-QRNSVT 487

Query: 481 IEIRRLPVGDGIWIARHK----------HLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQ 540
            ++R+L VGD +W+AR +           +  E VLD+I+ERK +DDL  SI D R+R+Q
Sbjct: 488 FDVRKLNVGDFLWVARERVTPVPGQLRPPVGKELVLDYIIERKRMDDLCGSIIDGRFREQ 547

Query: 541 KLKLLRCGLKRMIYLVE----GDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKK 600
           K +L RCGL++ IYLVE       + S    +++ A   T++++GF V+R     ++   
Sbjct: 548 KFRLKRCGLRKPIYLVEECGSAAAHLSIPESTLQQAIVNTQVVDGFFVKRVQDAKESAAY 566

Query: 601 YGYLTRAITQYYVL--------------QASDERLSHSDACPRL--NEF----IK-KCQE 644
              +TR + + Y                +A  E++  + +C  +   EF    IK KCQ 
Sbjct: 608 LTIMTRYLQKLYQNCTLLCRSRELEGDGEAESEKMVANLSCSLMAFTEFNYGAIKNKCQ- 566

BLAST of HG10015701 vs. ExPASy TrEMBL
Match: E5GCK8 (Endonuclease OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 568/644 (88.20%), Postives = 595/644 (92.39%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK SQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLS
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S+KNFDN KGSSILESCDIS+HSANYLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QV++SFKEVS+DNPNKD SSLWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEG 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PK VVND+G+RSFN RACSSSNPSI ESRRD VK++ NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFA HSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERK+IDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVS I
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSSI 617

BLAST of HG10015701 vs. ExPASy TrEMBL
Match: A0A1S3BFN6 (crossover junction endonuclease MUS81 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489485 PE=3 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 565/647 (87.33%), Postives = 596/647 (92.12%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQRSPV CPENEDLAAFMWQKRLEMA TPKGISDKVDMTLYKAYFNICNSK  I+SMK
Sbjct: 1   MDRQRSPVFCPENEDLAAFMWQKRLEMAATPKGISDKVDMTLYKAYFNICNSKVSIRSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+QDFFKSGSEGSGPEELAGKGKKTKGT+RYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQDFFKSGSEGSGPEELAGKGKKTKGTRRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK SQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLLS
Sbjct: 181 KGKPSQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLLS 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S+KNFDN KGSSILESCDIS+HSANYLDSDVEVTSPS+S+ ++NK+IDVPLESLERF+RM
Sbjct: 241 SSKNFDNIKGSSILESCDISNHSANYLDSDVEVTSPSVSQGTKNKTIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QV++SFKEVS+DNPNKD SSLWPAVLCRLREDLVYGQVEAVGRENRSMG  SH G
Sbjct: 301 GYSKHQVINSFKEVSKDNPNKDTSSLWPAVLCRLREDLVYGQVEAVGRENRSMG--SHEG 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PK VVND+G+RSFN RACSSSNPSI ESRRD VK++ NILSMPPLSFGEKFED YNVV
Sbjct: 361 SEPKNVVNDNGERSFNSRACSSSNPSIIESRRDSVKEHTNILSMPPLSFGEKFEDCYNVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFA HSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHK+LDNEYVLDFIV
Sbjct: 421 LILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKYLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERK+IDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKDIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASD+R+SHSD CPRL++FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDICDR 648
           VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLL  ++ ++
Sbjct: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLDGNVTEQ 620

BLAST of HG10015701 vs. ExPASy TrEMBL
Match: A0A6J1GYZ8 (crossover junction endonuclease MUS81 OS=Cucurbita moschata OX=3662 GN=LOC111458164 PE=3 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 562/644 (87.27%), Postives = 590/644 (91.61%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q  FK+GSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVIFKNGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK  QFGSS RDWYSGW+CMKTL+SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTRDWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S KNFD  KGSSILESC ISD SAN+LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RM
Sbjct: 241 SEKNFDTMKGSSILESCGISDRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRS GSS+  G
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSTGSSNE-G 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PKIVV+D+G+RSFNLRACSSSN SITESR+D VK+N NILSMPPLSFGEKFED+Y+VV
Sbjct: 361 HVPKIVVSDNGERSFNLRACSSSNHSITESRKDVVKENTNILSMPPLSFGEKFEDAYSVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFANHSSRSRRMIENICSQFKI+IEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFANHSSRSRRMIENICSQFKIEIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASDERLSH D CPRL+ FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERLSHFDVCPRLDVFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEE+AVAVLDLYPTLLSLARAYSLL  ++
Sbjct: 601 VSDVFALQLMQVPQVTEEIAVAVLDLYPTLLSLARAYSLLDDNV 618

BLAST of HG10015701 vs. ExPASy TrEMBL
Match: A0A6J1JV85 (crossover junction endonuclease MUS81 OS=Cucurbita maxima OX=3661 GN=LOC111488639 PE=3 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 559/644 (86.80%), Postives = 587/644 (91.15%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MDRQR PVVCPENEDLAAFMWQKRLEMAETPKG SDKVDMTLYKAYFN+CNSK  IKSMK
Sbjct: 1   MDRQRIPVVCPENEDLAAFMWQKRLEMAETPKGTSDKVDMTLYKAYFNVCNSKDSIKSMK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLITL 120
           DLSQIKGVGKWILKQ+Q FFKSGSEGSGPEE+AGKGKKTKGTKRYVPQKNSVAYALLITL
Sbjct: 61  DLSQIKGVGKWILKQVQVFFKSGSEGSGPEEVAGKGKKTKGTKRYVPQKNSVAYALLITL 120

Query: 121 YRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEKG 180
           Y                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEKG
Sbjct: 121 Y-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEKG 180

Query: 181 KGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLLS 240
           KGK  QFGSS R+WYSGW+CMKTL+SRGLVVKS+CPAKYMLTEEGQ VARECLMRSGLL+
Sbjct: 181 KGKPGQFGSSTREWYSGWNCMKTLVSRGLVVKSSCPAKYMLTEEGQQVARECLMRSGLLN 240

Query: 241 STKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLRM 300
           S KNFD  KGSSILESCDIS  SAN+LDSDVEVTSPS+ +SSQNKSIDVPLESLERF+RM
Sbjct: 241 SEKNFDTMKGSSILESCDISGRSANHLDSDVEVTSPSICRSSQNKSIDVPLESLERFMRM 300

Query: 301 GYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHMG 360
           GYSK+QVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENR  GSS+  G
Sbjct: 301 GYSKHQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRLTGSSNE-G 360

Query: 361 NGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNVV 420
           + PKIV++D+G+RSFNLRACSSSN SITES +D VK+NMNILSMPPLSFGEKFED+Y+VV
Sbjct: 361 HVPKIVISDNGERSFNLRACSSSNQSITESCKDVVKENMNILSMPPLSFGEKFEDAYSVV 420

Query: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480
           LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV
Sbjct: 421 LILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFIV 480

Query: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540
           ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE
Sbjct: 481 ERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEILE 540

Query: 541 GFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKMT 600
           GFDVQRTSGLSDTLKKYGYLT AITQYY+LQASDER SH D CPRL+ FIKKCQELDKMT
Sbjct: 541 GFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDERPSHFDVCPRLDAFIKKCQELDKMT 600

Query: 601 VSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDI 645
           VSDVFALQLMQVPQVTEE+AV VLDLYPTLLSLARAYSLL  ++
Sbjct: 601 VSDVFALQLMQVPQVTEEIAVTVLDLYPTLLSLARAYSLLDDNV 618

BLAST of HG10015701 vs. ExPASy TrEMBL
Match: A0A0A0KWV2 (ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623550 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 557/648 (85.96%), Postives = 591/648 (91.20%), Query Frame = 0

Query: 1   MDRQRSPVV-CPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSM 60
           MDRQRSP V CPENEDLA FMWQKRLEMA TPKGISDKVDMTLYKAY N+CNSK  I SM
Sbjct: 1   MDRQRSPAVFCPENEDLAEFMWQKRLEMAATPKGISDKVDMTLYKAYLNVCNSKVSITSM 60

Query: 61  KDLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGKGKKTKGTKRYVPQKNSVAYALLIT 120
           KDLSQIKGVGKWILKQ+QDFFKSGS+GS PEELAGKGKKTKGT+RYVPQKNSVAYALLIT
Sbjct: 61  KDLSQIKGVGKWILKQVQDFFKSGSDGSEPEELAGKGKKTKGTRRYVPQKNSVAYALLIT 120

Query: 121 LYRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPEK 180
           LY                         RGTTNGNEYMRKQELIDAAEASGLSRVAI PEK
Sbjct: 121 LY-------------------------RGTTNGNEYMRKQELIDAAEASGLSRVAIAPEK 180

Query: 181 GKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGLL 240
           GKGK SQFGSS RDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQ VARECLMRSGLL
Sbjct: 181 GKGKPSQFGSSSRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQQVARECLMRSGLL 240

Query: 241 SSTKNFDNTKGSSILESCDISDHSANYLDSDVEVTSPSLSKSSQNKSIDVPLESLERFLR 300
           SS+KNFDNTKGSSILESCDIS+HSANYLDSDVEVTSPS+S+S+++KSIDVPLESLERF+R
Sbjct: 241 SSSKNFDNTKGSSILESCDISNHSANYLDSDVEVTSPSVSQSTKHKSIDVPLESLERFMR 300

Query: 301 MGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGSSSHM 360
           MGYSK+QV++SFKEVS+DNPNKDISSLWPAVLCRLREDLVYGQVEA+GRENRSMGSS   
Sbjct: 301 MGYSKHQVINSFKEVSKDNPNKDISSLWPAVLCRLREDLVYGQVEAIGRENRSMGSSLE- 360

Query: 361 GNGPKIVVNDDGKRSFNLRACSSSNPSITESRRDGVKDNMNILSMPPLSFGEKFEDSYNV 420
           G+ PK VVN +G+RSF+ RACSSSNPSI ESRRD VK N NILSMPPLSFGEKFED YNV
Sbjct: 361 GSEPKNVVNVNGERSFSSRACSSSNPSIIESRRDSVKGNTNILSMPPLSFGEKFEDCYNV 420

Query: 421 VLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWIARHKHLDNEYVLDFI 480
           VLILDDREQFA HSSRSRRMIENICSQFKIQIEIRRLPVGDGIW+ARHKHLDNEYVLDFI
Sbjct: 421 VLILDDREQFAKHSSRSRRMIENICSQFKIQIEIRRLPVGDGIWVARHKHLDNEYVLDFI 480

Query: 481 VERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEIL 540
           VERK+++DLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEIL
Sbjct: 481 VERKDVEDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPNSSEAAESIKTACFTTEIL 540

Query: 541 EGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSDACPRLNEFIKKCQELDKM 600
           EGFDVQRTSGLSDTLKKYGYLT AITQYY+LQASD+R+SHSD CPRL++FIKKCQELDKM
Sbjct: 541 EGFDVQRTSGLSDTLKKYGYLTHAITQYYILQASDDRVSHSDVCPRLDQFIKKCQELDKM 600

Query: 601 TVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLLVSDICDR 648
           TVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSL RAYSLL  ++ ++
Sbjct: 601 TVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLVRAYSLLDGNVTEQ 622

BLAST of HG10015701 vs. TAIR 10
Match: AT4G30870.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 628.6 bits (1620), Expect = 5.4e-180
Identity = 345/664 (51.96%), Postives = 448/664 (67.47%), Query Frame = 0

Query: 1   MDRQRSPVVCPENEDLAAFMWQKRLEMAETPKGISDKVDMTLYKAYFNICNSKAPIKSMK 60
           MD +R  V+CPEN  LAA++ QK+ E AE PKG+S+ ++ T  K Y ++C++K PI ++K
Sbjct: 1   MDDERR-VLCPENRGLAAYVLQKKQEYAEKPKGLSENLERTFVKGYRSVCDAKDPINTLK 60

Query: 61  DLSQIKGVGKWILKQIQDFFKSGSEGSGPEELAGK--GKKTKGTKRYVPQKNSVAYALLI 120
           DLSQIKG GKW++K ++ +F +  E S  E+L     GKK  G KRY+PQ+NSV YALLI
Sbjct: 61  DLSQIKGFGKWMVKLMKGYFDTAVESSEQEDLPDNRAGKKANGKKRYIPQRNSVGYALLI 120

Query: 121 TLYRYNGQIVISFVKFLFKFTCNIRSTIRGTTNGNEYMRKQELIDAAEASGLSRVAIGPE 180
           TL+                         R TTNG E+MRKQELIDAA+A+GLS   +GPE
Sbjct: 121 TLH-------------------------RRTTNGKEFMRKQELIDAADANGLSHAPVGPE 180

Query: 181 KGKGKASQFGSSPRDWYSGWSCMKTLISRGLVVKSNCPAKYMLTEEGQHVARECLMRSGL 240
           KGKGKA   G S R+WYSGWSCM TLI +GLVVKS+ PAKYMLT EG+ VA EC++RSGL
Sbjct: 181 KGKGKAG-LGHSKREWYSGWSCMTTLIQKGLVVKSSNPAKYMLTVEGREVANECILRSGL 240

Query: 241 LSSTK--NFDNTKGSSILESCDISDHSANY-LDSDVEVTSPSLSKSSQNKSIDVPLESLE 300
             S    + D    +   +     + + ++ +  ++    P     S      +P + LE
Sbjct: 241 PDSVDILSVDEMDPTPQAKKTPNQNPTCSFTMREELPYVDPRCRAQSA-----IPSDILE 300

Query: 301 RFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWPAVLCRLREDLVYGQVEAVGRENRSMGS 360
           +F   GYSK QV+ +F+EVS+ + +KD S+LW +V+C LR+  VY    +      S   
Sbjct: 301 KFTPFGYSKEQVVAAFREVSDGSGDKDPSTLWLSVMCHLRQAEVY---NSCPDSRNSKKD 360

Query: 361 SSHMGNGPKIVVNDDGKRSFNLRAC---SSSNPSITESRR-----------DGVKDNMNI 420
           SS         V+ +G R+   R+C   S+ NP  + S             DG K   NI
Sbjct: 361 SSGPFKSQIRQVDLEGSRAKKFRSCNDGSTLNPCSSGSSHAVKACSSSLASDGTKGITNI 420

Query: 421 LSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFKIQIEIRRLPVGDG 480
             +PPL FGE FE++Y+V+LILDDRE+FA   SRSR ++ENICS+F I+IE+RRLPVGD 
Sbjct: 421 PRLPPLQFGETFEEAYDVILILDDREKFATKGSRSRNIVENICSEFNIKIEVRRLPVGDC 480

Query: 481 IWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCGLKRMIYLVEGDPN 540
           IWIARHK+L+ EYVLDFI ERKN+DD+R SIRDNRYRDQKL+L R G K++IY++EGDPN
Sbjct: 481 IWIARHKYLETEYVLDFIAERKNVDDMRSSIRDNRYRDQKLRLQRSGFKKLIYILEGDPN 540

Query: 541 SSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYYVLQASDERLSHSD 600
            S+AAESIKTACFTTEILEGFDV RT GL +TL+KYGYLT++I QYY L+ +D   S   
Sbjct: 541 HSDAAESIKTACFTTEILEGFDVLRTHGLGETLRKYGYLTKSIYQYYKLRVNDNDQSKGA 600

Query: 601 A-CPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEEVAVAVLDLYPTLLSLARAYSLL 645
           A CP  + F+K+CQ+LDKMT+SDVFA+QLMQVPQVTEE+A+AVLD+YPTLLSLA AYS L
Sbjct: 601 ASCPSFDSFVKRCQDLDKMTISDVFAIQLMQVPQVTEEIAIAVLDMYPTLLSLASAYSHL 629

BLAST of HG10015701 vs. TAIR 10
Match: AT5G39770.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 7.7e-110
Identity = 255/621 (41.06%), Postives = 337/621 (54.27%), Query Frame = 0

Query: 31   PKGISDKVDMTLYKAYFNICNSKAPIKSMKDLSQIKGVGKWILKQIQDFFKSGSEGSGPE 90
            PKG+S+  +M   KAY N+C++K  I ++KDLS+IKG GK+++K +Q +F +G+  S  E
Sbjct: 762  PKGLSEYQEMKFVKAYRNVCDAKDSIDTLKDLSKIKGFGKYMVKLMQGYFVTGAASSEQE 821

Query: 91   ELAGK--GKKTKGTKRYVPQKNSVAYALLITLYRYNGQIVISFVKFLFKFTCNIRSTIRG 150
             L G   GKK KG++ Y PQ+N+ AYALL TL+R+                         
Sbjct: 822  ALPGNRAGKKAKGSEPYKPQRNTAAYALL-TLHRFK------------------------ 881

Query: 151  TTNGNEYMRKQELIDAAEASGLSRVAIGPEKGKGKASQFGSSPRDWYSGWSCMKTLISRG 210
            T NG + MRKQ+L+DAA+ASGLS V+I PEKGKGKA   G+S R+WYSGWSCM  LI +G
Sbjct: 882  TANGKKLMRKQKLMDAADASGLSHVSIAPEKGKGKAG-LGTSKREWYSGWSCMSKLIEKG 941

Query: 211  LVVKSNCPAKYMLTEEGQHVARECLMRSGLLSSTKNFDNTKGSSILESCDISDHSANYLD 270
            LVVK + PAKYMLT EG++VA +   RS            +G S + +   S  +  +L 
Sbjct: 942  LVVKFSNPAKYMLTVEGRNVADDLDPRS------------RGQSSIPA-GPSSRAQEFLA 1001

Query: 271  SDVEVTSPSLSKSSQNKSIDVPLESLERFLRMGYSKNQVLDSFKEVSEDNPNKDISSLWP 330
            +D                                                          
Sbjct: 1002 TDT--------------------------------------------------------- 1061

Query: 331  AVLCRLREDLVYGQVEAVGRENRSMGSSSHMGNGPKIVVNDDGKRSFNLRACSSSNPSIT 390
                        GQV+  G   +   SSS   +            S  L+ACSSS  S  
Sbjct: 1062 ------------GQVDLEGSRAKKFRSSSPCSS----------SISHALKACSSSLAS-- 1121

Query: 391  ESRRDGVKDNMNILSMPPLSFGEKFEDSYNVVLILDDREQFANHSSRSRRMIENICSQFK 450
                DG +    I  +PPL FGE FE+ Y+V+LILDDREQFA                  
Sbjct: 1122 ----DGTEGVTTIPRLPPLRFGEAFEEVYDVILILDDREQFATKG--------------- 1181

Query: 451  IQIEIRRLPVGDGIWIARHKHLDNEYVLDFIVERKNIDDLRCSIRDNRYRDQKLKLLRCG 510
                              HK+  +EYVLDFIVERKN+DD+R SI DNRY+DQKL+L R G
Sbjct: 1182 ------------------HKYQQDEYVLDFIVERKNVDDMRSSIMDNRYKDQKLRLQRSG 1225

Query: 511  LKRMIYLVEGDPNSSEAAESIKTACFTTEILEGFDVQRTSGLSDTLKKYGYLTRAITQYY 570
            +K++IY++EGDPN S+ AESIKTACFTTEILEGFDV RT+GL +TL+KYGYLT++I QYY
Sbjct: 1242 IKKLIYILEGDPNQSKTAESIKTACFTTEILEGFDVVRTNGLGETLRKYGYLTKSIYQYY 1225

Query: 571  VLQASDERLSHSDACPRLNEFIKKCQELDKMTVSDVFALQLMQVPQVTEE---------V 630
                +D++   +D+CP  ++F+++C +L KMT+SDVFA+QLMQV  + +           
Sbjct: 1302 KSLVNDDQRKVADSCPSYDDFVQRCPDLGKMTISDVFAIQLMQVLSLAKPSFGFLSATFF 1225

Query: 631  AVAVLDLYPTLLSLARAYSLL 641
            A+AVLD Y TL SLA AYS L
Sbjct: 1362 AIAVLDKYKTLRSLASAYSKL 1225