HG10015661 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10015661
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	Chr02: 28686140 .. 28688525 (-)
RNA-Seq Expression	HG10015661
Synteny	HG10015661

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGCTGGCAGAGGAATCTCAGGCTGCTCCAACATATGGAGCATCATCGTCCACGTCATCACCGGACGGTGGTTCGCGGTATTCGCTTCACTCCTAATCATGGCGACGGCTGGAACAGTGTTCATGTTCAGCATGTATTCCAACGACATAAAATTTGTTTTAGGTTACGACCAAACGACTTTAAATCTGTTGAGTTTTTTCAAAGATTTAGGTGGAAATGTTGGCATTTTGTCTGGACTTATTAGCGAGATTACGCCACCATGGGTAGTTCTATCCATCGGCGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCATTACTAAGCGGATCTCTACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAATCCTTTTCTAATACGGGTTCCCTTGTTACTTGCGTCAAGAACTTTTCTGAGAGTCGTGGTATTGTTCTCGGTAAGTAGTTTAATTATAAGTTTAGTTTATGAACTTTTAATTTTATTTTTTTATCTAGTCTTTGAACGTAAATTTTTTTAAAAAATTTAATTAAGTCTCAATTTTGTATATATTGTTTGAATTAATGATTCTCCACAAATGTATTAAATTATAAGTTTAGTTCTTAAAATTTAAACGTCAAAGTGAGCTCAGTTCAACTTGTGGTCATTGGCATGACTTCTTTTTTTTTTCCTCGAGATCGAAAGTTCAATCCTCCACCATGCAATTGTTCTACTAAAAAATACAAAAAATAGAGAAAGAAAAAAAAATATATACGAACTAGAGCGTGAAGTTCAGGATCTTAGACCGAAAATTCAATTTTCCAGCTATTAGATGAATTAATTTTCATAAAAATTGAATATGTCATAAACTTGTTTGACACAAAATTGAAAGTTGATTAATTTATTAGCATGTTATAATCTTGGAAAACTAAACTTATTGAATCCTTAATTATTTATTATTTTCTCCCTAAATTCAAACTTAGATTATGTAAACACTCCCATTGTTTAGTATTTTCATAACGATTCCAACATTAGATTCCATTTTTAAAATAAATAAATAAATAAATAACTACTAATCAAACTTAAACGTTAAAAAAATTTAGGTATCTTGAAGGGCTACGTCGGACTGAGCGGCGCCATAATCACACAGCTCTTCCACGCATTTTACGGCAGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTGTGCATTTTTACGAACAATTCGTATTATGAAAGTCATTCGACATCCAAATGAGCTTAAAGTCTTCTACAATTTCCTCTACATTTCTCTTGGCCTCGCCGGATTTCTAATGATAATGATCATCGTAGAAAGCAAAACAGAGTTCACGCAAAATGAATATGGTGGCAGCGCTGCCGTCGTCCTCTTCCTCCTATTCCTCCCACTCGCTGTCGTCATCATCGAAGAATACAATCTATGGAAACTCAAAACGACCCTCATTAAATCTCCAAATCCCTCTGTTCAAATTGTAACAGAGCAACTCCCAAAAACAGAGCATCCGAAACAAGAACAAAAAGAACCATCTTGTTGGACAACTATTTTCAATCCACCAGAGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGTGTCGGCGGAACGTTAACGGCGATCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTGGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCGTCTCAGAAATCGTTTTGACAAAATACAAATTCCCCCGTCCTTTAATGCTCTCTCTAACCCTTCTTTTTTCCTGCGTTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGTTATACGTTGCTTCCATCGTAATTGGATTCAGCTTTGGAGCACAATGGCCAGTGATTTTCGCCATAATTTCAGAGCTTTTCGGGTTGAAATATTACTCAACGCTTTACAATTTTGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTACGATAGAGAGGCAGAGAAGCAACTAATTGCAAAAGGGATAATTAGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATTGTAATCACTGGAGTTAGTTTGTTGGGTATGTTTGTTTCGTTGATTTTAGTGATTAGAACGAGGAGTTTTTACAAGAGTGATATTTATAAGAAGTTTCGAGATGAAGCTGAAAAGGAGGTTGTCGAGAATGGGGCTGTGGCGGCGGCGGGAGCAGCGGAAGAGACGAGAAAATGA

mRNA sequence

ATGATGGCTGGCAGAGGAATCTCAGGCTGCTCCAACATATGGAGCATCATCGTCCACGTCATCACCGGACGGTGGTTCGCGGTATTCGCTTCACTCCTAATCATGGCGACGGCTGGAACAGTGTTCATGTTCAGCATGTATTCCAACGACATAAAATTTGTTTTAGGTTACGACCAAACGACTTTAAATCTGTTGAGTTTTTTCAAAGATTTAGGTGGAAATGTTGGCATTTTGTCTGGACTTATTAGCGAGATTACGCCACCATGGGTAGTTCTATCCATCGGCGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCATTACTAAGCGGATCTCTACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAATCCTTTTCTAATACGGGTTCCCTTGTTACTTGCGTCAAGAACTTTTCTGAGAGTCGTGGTATTGTTCTCGGTATCTTGAAGGGCTACGTCGGACTGAGCGGCGCCATAATCACACAGCTCTTCCACGCATTTTACGGCAGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTGTGCATTTTTACGAACAATTCGTATTATGAAAGTCATTCGACATCCAAATGAGCTTAAAGTCTTCTACAATTTCCTCTACATTTCTCTTGGCCTCGCCGGATTTCTAATGATAATGATCATCGTAGAAAGCAAAACAGAGTTCACGCAAAATGAATATGGTGGCAGCGCTGCCGTCGTCCTCTTCCTCCTATTCCTCCCACTCGCTGTCGTCATCATCGAAGAATACAATCTATGGAAACTCAAAACGACCCTCATTAAATCTCCAAATCCCTCTGTTCAAATTGTAACAGAGCAACTCCCAAAAACAGAGCATCCGAAACAAGAACAAAAAGAACCATCTTGTTGGACAACTATTTTCAATCCACCAGAGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGTGTCGGCGGAACGTTAACGGCGATCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTGGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCGTCTCAGAAATCGTTTTGACAAAATACAAATTCCCCCGTCCTTTAATGCTCTCTCTAACCCTTCTTTTTTCCTGCGTTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGTTATACGTTGCTTCCATCGTAATTGGATTCAGCTTTGGAGCACAATGGCCAGTGATTTTCGCCATAATTTCAGAGCTTTTCGGGTTGAAATATTACTCAACGCTTTACAATTTTGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTACGATAGAGAGGCAGAGAAGCAACTAATTGCAAAAGGGATAATTAGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATTGTAATCACTGGAGTTAGTTTGTTGGGTATGTTTGTTTCGTTGATTTTAGTGATTAGAACGAGGAGTTTTTACAAGAGTGATATTTATAAGAAGTTTCGAGATGAAGCTGAAAAGGAGGTTGTCGAGAATGGGGCTGTGGCGGCGGCGGGAGCAGCGGAAGAGACGAGAAAATGA

Coding sequence (CDS)

ATGATGGCTGGCAGAGGAATCTCAGGCTGCTCCAACATATGGAGCATCATCGTCCACGTCATCACCGGACGGTGGTTCGCGGTATTCGCTTCACTCCTAATCATGGCGACGGCTGGAACAGTGTTCATGTTCAGCATGTATTCCAACGACATAAAATTTGTTTTAGGTTACGACCAAACGACTTTAAATCTGTTGAGTTTTTTCAAAGATTTAGGTGGAAATGTTGGCATTTTGTCTGGACTTATTAGCGAGATTACGCCACCATGGGTAGTTCTATCCATCGGCGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGCTCGCCATTACTAAGCGGATCTCTACCCCCAAAGTTTGGCAAATGTGTCTCTATATTTGTATTGGAGCTAATTCTCAATCCTTTTCTAATACGGGTTCCCTTGTTACTTGCGTCAAGAACTTTTCTGAGAGTCGTGGTATTGTTCTCGGTATCTTGAAGGGCTACGTCGGACTGAGCGGCGCCATAATCACACAGCTCTTCCACGCATTTTACGGCAGCGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTGTGCATTTTTACGAACAATTCGTATTATGAAAGTCATTCGACATCCAAATGAGCTTAAAGTCTTCTACAATTTCCTCTACATTTCTCTTGGCCTCGCCGGATTTCTAATGATAATGATCATCGTAGAAAGCAAAACAGAGTTCACGCAAAATGAATATGGTGGCAGCGCTGCCGTCGTCCTCTTCCTCCTATTCCTCCCACTCGCTGTCGTCATCATCGAAGAATACAATCTATGGAAACTCAAAACGACCCTCATTAAATCTCCAAATCCCTCTGTTCAAATTGTAACAGAGCAACTCCCAAAAACAGAGCATCCGAAACAAGAACAAAAAGAACCATCTTGTTGGACAACTATTTTCAATCCACCAGAGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGTGTCGGCGGAACGTTAACGGCGATCGACAATTTGGGTCAAATCGGAATGTCTTTGGGATACCCAAAAAGAAGCATTGGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCGTCTCAGAAATCGTTTTGACAAAATACAAATTCCCCCGTCCTTTAATGCTCTCTCTAACCCTTCTTTTTTCCTGCGTTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGTTATACGTTGCTTCCATCGTAATTGGATTCAGCTTTGGAGCACAATGGCCAGTGATTTTCGCCATAATTTCAGAGCTTTTCGGGTTGAAATATTACTCAACGCTTTACAATTTTGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTACGATAGAGAGGCAGAGAAGCAACTAATTGCAAAAGGGATAATTAGAAAAGCAGGGGAAGAATTAAAGTGCTTTGGAGGGGAATGCTTTAAGCTTTCATTCATTGTAATCACTGGAGTTAGTTTGTTGGGTATGTTTGTTTCGTTGATTTTAGTGATTAGAACGAGGAGTTTTTACAAGAGTGATATTTATAAGAAGTTTCGAGATGAAGCTGAAAAGGAGGTTGTCGAGAATGGGGCTGTGGCGGCGGCGGGAGCAGCGGAAGAGACGAGAAAATGA

Protein sequence

MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVESKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKTEHPKQEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK

Homology

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BLAST of HG10015661 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1011.9 bits (2615), Expect = 2.2e-291
Identity = 514/584 (88.01%), Postives = 548/584 (93.84%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GI GCS++ SI VHVITGRWF VFASLLIMA AG  +MF +YS+DIK VLGYDQT
Sbjct: 1   MVAGGGILGCSDMRSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSDDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGA+LNFFGYFMIWLA+T+RIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMC+YICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS AFLRTIRI+KVIRHPNELKVFYNFLYISLGLAGFLM+MII+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIIKVIRHPNELKVFYNFLYISLGLAGFLMLMIIIE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKT 300
           SKTEFTQNEYGGSAAVVL LLFLPLAVVIIEEYNLWKLKT  IKS NP VQIVTEQLPKT
Sbjct: 241 SKTEFTQNEYGGSAAVVLVLLFLPLAVVIIEEYNLWKLKTAFIKSSNPCVQIVTEQLPKT 300

Query: 301 EH----PKQEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAID 360
           EH    PKQEQKEPSCWTT+F+PPERGEDFTILQALFS+DMLILFLSA CGVGGTLTAID
Sbjct: 301 EHPKQTPKQEQKEPSCWTTVFSPPERGEDFTILQALFSIDMLILFLSATCGVGGTLTAID 360

Query: 361 NLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFS 420
           NLGQIGMSLGYPKRSI TFV+LVSIWNYLGR+ASGFVSEIV+TKYKFPRPL+LSLTLL S
Sbjct: 361 NLGQIGMSLGYPKRSISTFVSLVSIWNYLGRIASGFVSEIVVTKYKFPRPLILSLTLLLS 420

Query: 421 CVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIG 480
           CVGHLMIAFDVPNGLYVAS+VIGF FGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIG
Sbjct: 421 CVGHLMIAFDVPNGLYVASVVIGFCFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIG 480

Query: 481 LYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVS 540
           LY+LNVKVAGNFYDREAEKQL AKGI RKAG++LKCFGGECFKLSFIVIT V+LLG F+S
Sbjct: 481 LYILNVKVAGNFYDREAEKQLRAKGITRKAGKKLKCFGGECFKLSFIVITAVTLLGTFIS 540

Query: 541 LILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK 581
           LILVIRT+SFYKSDIYKKFRDEAE EV  NGAVA AGA EET+K
Sbjct: 541 LILVIRTKSFYKSDIYKKFRDEAETEVAGNGAVAPAGAGEETKK 584

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BLAST of HG10015661 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1008.8 bits (2607), Expect = 1.9e-290
Identity = 517/582 (88.83%), Postives = 545/582 (93.64%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MF +YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAVLNFFGYFMIWLA+T+RISTPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS A LRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKT 300
           SK EF QNEYGGSAAVVL LLFLPL+VVIIEEYNLWKLKT L+KSPNPSVQIVTE+LPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-EQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLG 360
           EHPKQ EQKEPSCWTTIFNPP+RGEDFTILQALFS DMLILF++AICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVG 420
           QIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLL SCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLIL 540
           LNVKVAGNFYDREAEKQL AKGIIRKAGEELKCFGGECFKLSFIVIT V+LLGM VSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEAE-KEVVENGAVAAAGAAEETRK 581
           VIRTRSFYKSDIYKKFRDEAE KEV  N  +  AG  EE RK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

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BLAST of HG10015661 vs. NCBI nr
Match: XP_004142499.3 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothetical protein Csa_008497 [Cucumis sativus])

HSP 1 Score: 999.6 bits (2583), Expect = 1.1e-287
Identity = 514/581 (88.47%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+A R ISGCS+I SI VHVITGRWF VFASLLIMA AG  +MF +YS+DIK VLGYDQT
Sbjct: 1   MVADRRISGCSDISSIGVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAVLNFFGYFMIWLA+T+RIS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGD 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS A LRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKT 300
           SK +F QNEYGGSAAVVL LL LPLAVVIIEEYNLWKLKT +IKSPNPSVQIVTE+LPKT
Sbjct: 241 SKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYNLWKLKTAVIKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQ 360
           EHPKQE+KEPSCWTTIF+PP+RGEDFTILQALFSVDMLILF++AICGVGGTLTAIDNLGQ
Sbjct: 301 EHPKQERKEPSCWTTIFSPPQRGEDFTILQALFSVDMLILFIAAICGVGGTLTAIDNLGQ 360

Query: 361 IGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGH 420
           IG++LGYPKRSI TFV+LVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLL SCVGH
Sbjct: 361 IGLALGYPKRSISTFVSLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVGH 420

Query: 421 LMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 480
           LMIAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSVASPIGLYVL
Sbjct: 421 LMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 480

Query: 481 NVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILV 540
           NVKVAGNFYDREAEKQL AK IIRKAGEELKCFGGECFKLSFIVITGV+LLGM VSLILV
Sbjct: 481 NVKVAGNFYDREAEKQLEAKRIIRKAGEELKCFGGECFKLSFIVITGVTLLGMLVSLILV 540

Query: 541 IRTRSFYKSDIYKKFRDEAE-KEVVENGAVAAAGAAEETRK 581
           IRTRSFYKSDIYKKFRDE E  EV  NG V AAG  EE RK
Sbjct: 541 IRTRSFYKSDIYKKFRDEVETTEVAGNGVVEAAGVPEERRK 581

[Close]

BLAST of HG10015661 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 998.0 bits (2579), Expect = 3.3e-287
Identity = 515/589 (87.44%), Postives = 544/589 (92.36%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MFS+YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAV+NFFGYFMIWLA+T++I+ PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS AFLRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLK----TTLIKSPNPSVQIVTEQ 300
           SKTEF+Q+ YGGSAAVVL LLFLPLAVVIIEEYNLW LK    T  I+SPN  VQIVTE+
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGT 360
           LPKTEH K     + QKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFL+AICGVGGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSL 420
           LTAIDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLFSCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSV 480
           TLL SCVGHLMIAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AKGI+RK GEELKCFGGECFKLSF+VITGV+LL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK 581
           GMFVSLILVIRTRSFYKSDIYKKFRDEAE E+  NGAV AAG  EETRK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

[Close]

BLAST of HG10015661 vs. NCBI nr
Match: XP_023535761.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.4 bits (2567), Expect = 8.1e-286
Identity = 511/589 (86.76%), Postives = 543/589 (92.19%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG G SGCS+I SI VHVITGRWF VFASLLIMA AG  +MFS+YS+DIK VLGYDQT
Sbjct: 1   MVAGGGTSGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAV+NFFGYFMIWLA+T++IS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTQKISAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS AFLRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLK----TTLIKSPNPSVQIVTEQ 300
           SKTEF+Q+ YGGSAAVVL LLFLPLAVVIIEEYNLW LK    T  I+SPN  VQIVTE+
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAATTAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGT 360
           +PKTEH K     + +K+PSCWTTIFNPPERGEDFTILQALFSVDMLILFL+AICGVGGT
Sbjct: 301 VPKTEHQKHIPKTEHKKQPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSL 420
           LTAIDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLFSCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSV 480
           TLL SCVGHLMIAFDVPNGLYVAS+VIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASVVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AKGI+RK GEELKCFGGECFKLSF+VITGV+LL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK 581
           GMFVSLILVIRTRSFYKSDIYKKFRDEAE E+  NGAV AAG  EETRK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAAGPPEETRK 589

[Close]

BLAST of HG10015661 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 3.3e-32
Identity = 138/538 (25.65%), Postives = 242/538 (44.98%), Query Frame = 0


Query: 24  RWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVGILSGLIS 83
           +W  + A++ I A+ GT F FS YS+ +K VLG  Q  LN L+   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 84  EITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLYICIGAN-SQSFSNTGSLV 143
              P  VVL   A + F GY + WL IT  I+ P  + +    C+ A  S  + NT   +
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFI 162

Query: 144 TCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISCAFLR 203
            C+++F  +R + L +   + G+S A+ +  F+A   S +   +LL   +P  +S A L 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALY 222

Query: 204 TIRIMKVI---------RHPNELKVFYNFLYISLGLAGFLMIMIIVESKTEFTQNEYGGS 263
            +     +         RH + +    N L +          +++  S T   +  + G+
Sbjct: 223 PVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFH-----LLLSSSSTSSARLNFIGA 282

Query: 264 AAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQL--PKTEHPKQEQKEPS 323
             +++F L  PL +V   +Y L  +   L    +  V +  ++L   KT    +   E  
Sbjct: 283 VVLLVFPLCAPL-LVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHM 342

Query: 324 CWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRS 383
                 N    G++ +    +  ++  + +++  CG    L   +NLGQI  SLG   ++
Sbjct: 343 GTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QN 402

Query: 384 IGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHLMIAF--DVPN 443
             T VT+ S +++ GR+ S    + +  +++  R    ++ LL + +   ++A       
Sbjct: 403 STTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 444 GLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFY 503
            L  A+ +IG S G  +    +I S+LFG       +N      PIG  +L   +A + Y
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG-SLLYGYIAASIY 522

Query: 504 DREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILVIRTRSFY 548
           +  A     +  I     + + C G +C+  +F+    +S+LG+  SL L IRT+  Y
Sbjct: 523 EANA-----SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

[Close]

BLAST of HG10015661 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 7.0e-06
Identity = 56/218 (25.69%), Postives = 97/218 (44.50%), Query Frame = 0


Query: 323 GEDFTILQALFSVDMLILFLSAICGVGGTLTAI-DNLGQIGMSL---GYPKRSIGTFVTL 382
           G +   L+  F+     LFL     +GG      +N+G I  ++        S  T V+L
Sbjct: 259 GHEGATLKEFFTDKTAWLFLLCFVFIGGPFEMFQNNMGAILDTVTVENADSPSFSTHVSL 318

Query: 383 VSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHLMI------AFDVPNGLY 442
            + ++ + R+  GF SE +  +    RP++LS+  L +   HLM+       FD      
Sbjct: 319 FATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVIALVAACIHLMVPSGIFTVFDNAKYFS 378

Query: 443 VASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDRE 502
           V +IV GFS+G+ + ++  I+++++G+    T++         G ++L + V    Y   
Sbjct: 379 VVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW---------GSFILALAVGSLGYGLL 438

Query: 503 AEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSL 531
             K   A   +        C G  C+ L+F VITG  L
Sbjct: 439 FAKVYDAASEVGVGSMSQVCSGVHCYGLTF-VITGTGL 464

[Close]

BLAST of HG10015661 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.0e-291
Identity = 517/582 (88.83%), Postives = 545/582 (93.64%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MF +YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAVLNFFGYFMIWLA+T+RISTPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS A LRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKT 300
           SK EF QNEYGGSAAVVL LLFLPL+VVIIEEYNLWKLKT L+KSPNPSVQIVTE+LPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-EQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLG 360
           EHPKQ EQKEPSCWTTIFNPP+RGEDFTILQALFS DMLILF++AICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVG 420
           QIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLL SCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLIL 540
           LNVKVAGNFYDREAEKQL AKGIIRKAGEELKCFGGECFKLSFIVIT V+LLGM VSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEAE-KEVVENGAVAAAGAAEETRK 581
           VIRTRSFYKSDIYKKFRDEAE KEV  N  +  AG  EE RK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

[Close]

BLAST of HG10015661 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.0e-291
Identity = 517/582 (88.83%), Postives = 545/582 (93.64%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MF +YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAVLNFFGYFMIWLA+T+RISTPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG+
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS A LRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKT 300
           SK EF QNEYGGSAAVVL LLFLPL+VVIIEEYNLWKLKT L+KSPNPSVQIVTE+LPKT
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 EHPKQ-EQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLG 360
           EHPKQ EQKEPSCWTTIFNPP+RGEDFTILQALFS DMLILF++AICGVGGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVG 420
           QIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPL+LSLTLL SCVG
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLSCVG 420

Query: 421 HLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYV 480
           HL+IAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSVASPIGLY+
Sbjct: 421 HLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYI 480

Query: 481 LNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLIL 540
           LNVKVAGNFYDREAEKQL AKGIIRKAGEELKCFGGECFKLSFIVIT V+LLGM VSL+L
Sbjct: 481 LNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVSLVL 540

Query: 541 VIRTRSFYKSDIYKKFRDEAE-KEVVENGAVAAAGAAEETRK 581
           VIRTRSFYKSDIYKKFRDEAE KEV  N  +  AG  EE RK
Sbjct: 541 VIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

[Close]

BLAST of HG10015661 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.6e-287
Identity = 515/589 (87.44%), Postives = 544/589 (92.36%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MFS+YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAV+NFFGYFMIWLA+T++I+ PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS AFLRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MIIVE
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLK----TTLIKSPNPSVQIVTEQ 300
           SKTEF+Q+ YGGSAAVVL LLFLPLAVVIIEEYNLW LK    T  I+SPN  VQIVTE+
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPKTEHPK-----QEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGT 360
           LPKTEH K     + QKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFL+AICGVGGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSL 420
           LTAIDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 420

Query: 421 TLLFSCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSV 480
           TLL SCVGHLMIAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSV
Sbjct: 421 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 480

Query: 481 ASPIGLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLL 540
           ASPIGLYVLNVKVAGNFYDREAEKQL AKGI+RK GEELKCFGGECFKLSF+VITGV+LL
Sbjct: 481 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 540

Query: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK 581
           GMFVSLILVIRTRSFYKSDIYKKFRDEAE E+  NGAV AAG  EETRK
Sbjct: 541 GMFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

[Close]

BLAST of HG10015661 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.3e-285
Identity = 510/585 (87.18%), Postives = 539/585 (92.14%), Query Frame = 0


Query: 1   MMAGRGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQT 60
           M+AG GISGCS+I SI VHVITGRWF VFASLLIMA AG  +MFS+YS+DIK VLGYDQT
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  TLNLLSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVW 120
           TLNLLSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAV+NFFGYFMIWLA+T +IS PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVW 120

Query: 121 QMCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGS 180
           QMCLYICIGANSQSF+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVE 240
           DTKSLILLIGWLPAAIS AFLRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MII+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIE 240

Query: 241 SKTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKT-----TLIKSPNPSVQIVTE 300
           SKTEF+Q+ YGGSAAVVL LLFLPLAVVIIEEYNLW LKT       I+SPN  VQIVTE
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTE 300

Query: 301 QLPKTEHPKQEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAI 360
           ++PKTEH    QK+PSCWTTIFNPPERGEDFTILQALFSVDM ILFL+AICGVGGTLTAI
Sbjct: 301 KVPKTEH----QKQPSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGTLTAI 360

Query: 361 DNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLF 420
           DNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGFVSEIVLTKYKFPRPLMLS+TLL 
Sbjct: 361 DNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLV 420

Query: 421 SCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPI 480
           SCVGHLMIAFDVPNGLYVASIVIGF FGAQWP+IFAIISELFGLKYYSTLYNFGSVASPI
Sbjct: 421 SCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPI 480

Query: 481 GLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFV 540
           GLYVLNVKVAGNFYDREAEKQL AKGI+RK GEELKCFGGECFKLSF+VITGV+LLGMFV
Sbjct: 481 GLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLGMFV 540

Query: 541 SLILVIRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETRK 581
           SLILVIRTRSFYKSDIYKKFRDEAE E+  NGAV AA   EETRK
Sbjct: 541 SLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAAEPPEETRK 581

[Close]

BLAST of HG10015661 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 1.3e-276
Identity = 499/579 (86.18%), Postives = 531/579 (91.71%), Query Frame = 0


Query: 5   RGISGCSNIWSIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNL 64
           R ISG SNI +I VH+ITGRWF VFASLLIMA AG  +MFS+YSNDIK VLGYDQTTLNL
Sbjct: 10  RAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNL 69

Query: 65  LSFFKDLGGNVGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCL 124
           LSFFKDLG NVG+LSGLI+E+TPPWVVLSIGAVLNFFGYFMIWLA+TKRISTPKVWQMCL
Sbjct: 70  LSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCL 129

Query: 125 YICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKS 184
           YIC+GANSQSF NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQLFHAFYGSDTKS
Sbjct: 130 YICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKS 189

Query: 185 LILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVESKTE 244
           LILLIGWLPAAIS AFLRTIRIMKVIR PNELKVFYNFLYISL LAGFLM+MII+ESK  
Sbjct: 190 LILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKLI 249

Query: 245 FTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQ--LPKTEH 304
           F+Q E+GGSAAVVL LLFLPLAVVI+EEYNL KLK+  I  PNP +QI+TE+   PKTE 
Sbjct: 250 FSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNP-LQILTEKPNPPKTEP 309

Query: 305 PKQ--EQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQ 364
            KQ  +QK+PSCWTTIF+PPERGEDFTILQALFS+DM ILF SAICGVGGTLTAIDNLGQ
Sbjct: 310 QKQTRQQKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVGGTLTAIDNLGQ 369

Query: 365 IGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGH 424
           IGMSLGYPKRSI TFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLL SCVGH
Sbjct: 370 IGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGH 429

Query: 425 LMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVL 484
           L+IAF VPNGLYVASIVIGF FGAQWP++FAIISELFGLKYYSTLYNFGSVASPIGLYVL
Sbjct: 430 LIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFGSVASPIGLYVL 489

Query: 485 NVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILV 544
           NVKVAGN YD+EA KQL AKG++RK GEELKC+GGECFK SF+VITGV+LLGMFVSLILV
Sbjct: 490 NVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVTLLGMFVSLILV 549

Query: 545 IRTRSFYKSDIYKKFRDEAEKEVVENGAVAAAGAAEETR 580
           IRTR FYKSDIYKKFRDEAE EVV NGAV AAGA EETR
Sbjct: 550 IRTRGFYKSDIYKKFRDEAEAEVVGNGAVTAAGAPEETR 587

[Close]

BLAST of HG10015661 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 789.3 bits (2037), Expect = 2.2e-228
Identity = 401/571 (70.23%), Postives = 468/571 (81.96%), Query Frame = 0


Query: 15  SIIVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGN 74
           S+ + ++TGRWF  F SLLIM+TAG  +MF +YS DIK  LGYDQTTLNLLSFFKDLG N
Sbjct: 12  SLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGAN 71

Query: 75  VGILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLYICIGANSQS 134
           VG+L+GL++E+TPPW +L IGA+LNFFGYFMIWLA+T+RIS P+VW MCLYIC+GANSQS
Sbjct: 72  VGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQS 131

Query: 135 FSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPA 194
           F+NTGSLVTCVKNF ESRG+VLGILKGYVGLSGAIITQL+ AFYG DTK LIL+IGWLPA
Sbjct: 132 FANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPA 191

Query: 195 AISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVESKTEFTQNEYGGSA 254
            +S AFLRTIRIMKV R  NELKVFYNFLYISLGLA FLM++II+   + FTQ+E+GGSA
Sbjct: 192 IVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSA 251

Query: 255 AVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKTEHPK------QEQK 314
           AVV+ LL LP+ VVI+EE  LWK K   +  P P + +VTE+ PK +  +      +E K
Sbjct: 252 AVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAP-INVVTEK-PKLDSSEFKDDDGEESK 311

Query: 315 E-------PSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQI 374
           E       PSCWTT+FNPPERG+D+TILQALFSVDMLILFL+ ICGVGGTLTAIDNLGQI
Sbjct: 312 EVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQI 371

Query: 375 GMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHL 434
           G SLGYPKRS+ TFV+LVSIWNY GRV SG VSEI L KYKFPRPLML++ LL SC GHL
Sbjct: 372 GNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHL 431

Query: 435 MIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLN 494
           +IAF+VP GLYVAS++IGF FGAQWP++FAIISE+FGLKYYSTLYNFGSVASPIG Y+LN
Sbjct: 432 LIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLN 491

Query: 495 VKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILVI 554
           V+VAG  YD EA KQ  A G  R  G++L C G  CFKLSFI+I  V+L G+ VS++LVI
Sbjct: 492 VRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVI 551

Query: 555 RTRSFYKSDIYKKFRDEAEKEVVENGAVAAA 573
           RT+ FYKSDIYKKFR++A    +E  A AAA
Sbjct: 552 RTKKFYKSDIYKKFREKALAAEMEMAAPAAA 580

[Close]

BLAST of HG10015661 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 672.2 bits (1733), Expect = 3.8e-193
Identity = 332/552 (60.14%), Postives = 418/552 (75.72%), Query Frame = 0


Query: 17  IVHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVG 76
           ++H   GRWF VFAS LIMA AG  ++F  YS DIK  LGYDQTTLNLL FFKDLG NVG
Sbjct: 10  LIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVG 69

Query: 77  ILSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLYICIGANSQSFS 136
           +LSGLI+E+TP W VL+IG+ +NF GYFMIWL +T +++ PKVWQMCLYICIGANSQ+F+
Sbjct: 70  VLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFA 129

Query: 137 NTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAI 196
           NTG+LVTCVKNF ESRG++LG+LKGYVGLSGAI TQL+ A YG D+KSLILLI WLPAA+
Sbjct: 130 NTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAV 189

Query: 197 SCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVESKTEFTQNEYGGSAAV 256
           S  F+  IR  KV+R  NEL VFY FLYIS+ LA FLM M I E +  F++  Y  SA +
Sbjct: 190 SLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATI 249

Query: 257 VLFLLFLPLAVVIIEEYNLWKLKTTLIKSPN------PSVQIVTEQLPKTEHPKQEQKEP 316
              LLF+PL V + +E  +W +    I+ P+      P  ++  +Q    +   +E++  
Sbjct: 250 CCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETK 309

Query: 317 SCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKR 376
           SC++T+F+PP RGED+TILQAL S DM+ILF++  CG+G +LTA+DNLGQIG SLGYP  
Sbjct: 310 SCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNH 369

Query: 377 SIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHLMIAFDVPNG 436
           ++ +FV+LVSIWNY GRV SGFVSE +L KYK PRPLM++L LL SC GHL+IAF VP  
Sbjct: 370 TVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGS 429

Query: 437 LYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYD 496
           +Y+ASI++GFSFGAQ P++FAIISELFGLKYYSTL+N G +ASP+G Y+LNV+V G  YD
Sbjct: 430 VYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYD 489

Query: 497 REAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILVIRTRSFYKSD 556
           +EA KQL A+G+ RK  ++L C G +C+KL F+++  V+  G  VSL L IRTR FYK D
Sbjct: 490 KEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGD 549

Query: 557 IYKKFRDEAEKE 563
           IYKKFR+  E E
Sbjct: 550 IYKKFRESPESE 561

[Close]

BLAST of HG10015661 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.6e-90
Identity = 206/576 (35.76%), Postives = 320/576 (55.56%), Query Frame = 0


Query: 18  VHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVGI 77
           V  I  RW    A++ I + AG  ++F   S  IK  L Y+Q  L+ L   KDLG +VG 
Sbjct: 10  VSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGF 69

Query: 78  LSGLISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLYICIGANSQSFSN 137
           ++G +SEI P W  L +GAV N  GY  +WL +T R     +W MC+ I +G N +++ N
Sbjct: 70  IAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFN 129

Query: 138 TGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAIS 197
           TG+LV+ V+NF +SRG V+GILKG+ GL GAII+Q++   + S+  SLIL++   PA + 
Sbjct: 130 TGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVV 189

Query: 198 CA---FLRTIRIMKVIRHPNELKVFYNFLY-ISLGLAGFLMIMIIVESKTEFTQNEYGGS 257
                F+R +   K IR  +     + F+Y + L LA +LM +++++     + N     
Sbjct: 190 VCLMFFIRPVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVF 249

Query: 258 AAVVLFLLFLPLAVVI--------------IEEYNLWKLKTTL--IKSPNPSVQIVTEQ- 317
             V+  +L +P+ V I              IEE  + K +     +++P+  +  V ++ 
Sbjct: 250 TIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEK 309

Query: 318 ------LPKTEHPK----------QEQKEPSCWTTIFNPPERGEDFTILQALFSVDMLIL 377
                 LP +E  K          Q   E +        P RGEDFT+ QAL   D  ++
Sbjct: 310 PKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLI 369

Query: 378 FLSAICGVGGTLTAIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTK 437
           F S + G G  LT IDNLGQ+  SLGY    +   V+++SIWN+LGR+  G+ SE+V+  
Sbjct: 370 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRD 429

Query: 438 YKFPRPLMLSLTLLFSCVGHLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLK 497
           Y +PRP+ +++  L   VGH+  A+  P  +Y+ +++IG  +GA W ++ A  SELFGLK
Sbjct: 430 YAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLK 489

Query: 498 YYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKL 557
            +  LYNF ++A+P G  V +  +A + YDREAE+Q  A G +    + L+C G  CF L
Sbjct: 490 KFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFL 549

[Close]

BLAST of HG10015661 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.8e-89
Identity = 200/549 (36.43%), Postives = 311/549 (56.65%), Query Frame = 0


Query: 20  VITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVGILS 79
           ++  +W A+ AS+ I  +AG  + F +YS  +K    YDQ+TL+ +S FKD+GGNVG+LS
Sbjct: 5   ILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVLS 64

Query: 80  GLISEITP--------------PWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQMCLY 139
           GL+                   PWVV+ IGA+LNF GYF++W ++T  I  P V  MCL+
Sbjct: 65  GLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCLF 124

Query: 140 ICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSDTKSL 199
           + I A S +F NT ++V+ ++NF++  G  +GI+KG+VGLSGA++ QL+      D K+ 
Sbjct: 125 MFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKTF 184

Query: 200 ILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVESKTEF 259
           ILL+  +P+ +S   +  +R+ K     +E K       +SL +A +LMI II++S    
Sbjct: 185 ILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSL 244

Query: 260 TQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKTEHPKQ 319
                  + AV+L LL  PL V +       +     I+ P  SV            P  
Sbjct: 245 PSWANAVTLAVLLVLLSSPLLVAV-------RAHRDSIEKPLSSVY----------SPLV 304

Query: 320 EQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSL 379
           +  E +    I    E  +   +LQA+ +VD  +LFL+ ICG+G  ++ I+N+ QIG SL
Sbjct: 305 DNLEATTSGEILMLDE-DKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESL 364

Query: 380 GYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVGHLMIAF 439
            Y    I + + L +IWN++GR   G+VS+ +L +  +PRPL+++ TL    +GHL+IA 
Sbjct: 365 RYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIAS 424

Query: 440 DVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVA 499
                LY  SI++G  +G+QW ++  I SELFG+K+  T+YN  S+ASP+G Y+ +V++ 
Sbjct: 425 GFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLI 484

Query: 500 GNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLILVIRTRS 555
           G  YDR               GE   C+G  CF+L+++VI  V+ LG  VS +LV RT++
Sbjct: 485 GYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKT 521

[Close]

BLAST of HG10015661 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 9.7e-88
Identity = 193/555 (34.77%), Postives = 309/555 (55.68%), Query Frame = 0


Query: 18  VHVITGRWFAVFASLLIMATAGTVFMFSMYSNDIKFVLGYDQTTLNLLSFFKDLGGNVGI 77
           + ++  +W A+ AS+ I   +G  + F +YS  +K    YDQ+TL+ +S FKD+G N G+
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 78  LSGL----------------ISEITPPWVVLSIGAVLNFFGYFMIWLAITKRISTPKVWQ 137
            SGL                I     PWVVL++GA+  F GYF+IW ++T  I  P V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 138 MCLYICIGANSQSFSNTGSLVTCVKNFSESRGIVLGILKGYVGLSGAIITQLFHAFYGSD 197
           MCL++ + A SQ+F NT ++V+ V+NF++  G  +GI+KG++GLSGAI+ QL+      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 198 TKSLILLIGWLPAAISCAFLRTIRIMKVIRHPNELKVFYNFLYISLGLAGFLMIMIIVES 257
             S ILL+   P  +S   +  +RI +     ++ K       +SL +A +LMI+II+  
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIIL-- 240

Query: 258 KTEFTQNEYGGSAAVVLFLLFLPLAVVIIEEYNLWKLKTTLIKSPNPSVQIVTEQLPKTE 317
           K  F  + +     +V  L+ L L ++I        ++ T+    +P +       PK  
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLI-----SSPKAT 300

Query: 318 HPKQEQKEPSCWTTIFNPPERG--EDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLG 377
               +  E        +  E G  E+  +LQA+  +   +LFL+ ICG+G  L+ I+N+ 
Sbjct: 301 TSGNQSSEGD------SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIR 360

Query: 378 QIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLMLSLTLLFSCVG 437
           QIG SL Y    I + V+L SIWN+LGR  +G+ S+ +L K  +PRPL+++ TL    +G
Sbjct: 361 QIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIG 420

Query: 438 HLMIAFDVPNGLYVASIVIGFSFGAQWPVIFAIISELFGLKYYSTLYNFGSVASPIGLYV 497
           HL+IA      LYV S+++G  +G+QW ++  I SELFG+++  T++N  SVASPIG Y+
Sbjct: 421 HLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYI 480

Query: 498 LNVKVAGNFYDREAEKQLIAKGIIRKAGEELKCFGGECFKLSFIVITGVSLLGMFVSLIL 555
            +V++ G  YD+ A            +GE   C+G  CF+LSFI++  V+  G  V+++L
Sbjct: 481 FSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVL 529

The following BLAST results are available for this feature:

BLAST of HG10015661 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893353.1	2.2e-291	88.01	LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
XP_008446819.1	1.9e-290	88.83	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 ...	[more]
XP_004142499.3	1.1e-287	88.47	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothet...	[more]
XP_022956604.1	3.3e-287	87.44	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
XP_023535761.1	8.1e-286	86.76	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10015661 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	3.3e-32	25.65	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q6CGU8	7.0e-06	25.69	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]

BLAST of HG10015661 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFW3	9.0e-291	88.83	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BG09	9.0e-291	88.83	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ...	[more]
A0A6J1GZI8	1.6e-287	87.44	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K186	4.3e-285	87.18	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A6J1FZF2	1.3e-276	86.18	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326...	[more]

BLAST of HG10015661 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39210.1	2.2e-228	70.23	Major facilitator superfamily protein	[more]
AT2G28120.1	3.8e-193	60.14	Major facilitator superfamily protein	[more]
AT5G14120.1	1.6e-90	35.76	Major facilitator superfamily protein	[more]
AT1G18940.1	1.8e-89	36.43	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G74780.1	9.7e-88	34.77	Nodulin-like / Major Facilitator Superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 333..548 e-value: 1.5E-8 score: 36.2
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 25..545
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 24..269 e-value: 3.5E-91 score: 305.1
None	No IPR available	PANTHER	PTHR21576:SF122	MFS TRANSPORTER	coord: 10..572
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 10..572
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 23..533 e-value: 5.66519E-114 score: 342.694

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10015661.1	HG10015661.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0099587	inorganic ion import across plasma membrane
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity