HG10015593 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10015593
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Chr02: 27950084 .. 27950455 (-)
RNA-Seq Expression	HG10015593
Synteny	HG10015593

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAATCTCTCTAGATCTGTCTCTGTTTTTCTCTGATCCTGGAAACAGATAA

mRNA sequence

ATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAATCTCTCTAGATCTGTCTCTGTTTTTCTCTGATCCTGGAAACAGATAA

Coding sequence (CDS)

ATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAATCTCTCTAGATCTGTCTCTGTTTTTCTCTGATCCTGGAAACAGATAA

Protein sequence

MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISLDLSLFFSDPGNR

Homology

[Close]

BLAST of HG10015593 vs. NCBI nr
Match: XP_038892898.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 187.6 bits (475), Expect = 6.5e-44
Identity = 96/111 (86.49%), Postives = 101/111 (90.99%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLS 60
           MDT+SEF FPPPSSLFVNVMTVVSSAVV VVGLSE RGK+MKYSKFWN TNNAP SSNLS
Sbjct: 1   MDTVSEFFFPPPSSLFVNVMTVVSSAVVVVVGLSETRGKNMKYSKFWNNTNNAPKSSNLS 60

Query: 61  AKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           A+LGMLLAY PAFLVG+V LWLFPNEDRRI LLKSALILHF KRNLEV+ L
Sbjct: 61  ARLGMLLAYMPAFLVGVVLLWLFPNEDRRILLLKSALILHFFKRNLEVLFL 111

[Close]

BLAST of HG10015593 vs. NCBI nr
Match: XP_008446724.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo] >KAA0034633.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa] >TYK09184.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 176.4 bits (446), Expect = 1.5e-40
Identity = 92/114 (80.70%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNA---PNSS 60
           MD LSEFVFPPPSS FVNVMTVVSSA+VAVVGLSEARGKH++YSKFWN  NNA     S 
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNANTDRKSF 60

Query: 61  NLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           NLSAKLGMLLAY PAFLVG+VS WLFPNEDRRI +LKSAL+LHF KRNLEV+ L
Sbjct: 61  NLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFL 114

[Close]

BLAST of HG10015593 vs. NCBI nr
Match: XP_004135050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KGN52161.1 hypothetical protein Csa_008531 [Cucumis sativus])

HSP 1 Score: 172.9 bits (437), Expect = 1.7e-39
Identity = 90/113 (79.65%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFW--NTTNNAPNSSN 60
           MDTLSEFVFPPP+S FVNVMTVVSSA+VAVVGLSEARGKH+KYSKFW  N  N    S N
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNANTDRKSFN 60

Query: 61  LSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           LSAKLGML+AY PAFL G+VSLWLFP+EDRRI LLKSAL+LHF KRNLEV+ L
Sbjct: 61  LSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFL 113

[Close]

BLAST of HG10015593 vs. NCBI nr
Match: XP_022150685.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Momordica charantia])

HSP 1 Score: 152.1 bits (383), Expect = 3.0e-33
Identity = 78/113 (69.03%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSN-- 60
           M+ LSEF+FP P SLFVNVMTVVSSAVV  +G+SEA+GKH++YSKFWN TN AP SS+  
Sbjct: 3   MNALSEFLFPAPPSLFVNVMTVVSSAVVTAIGISEAKGKHLQYSKFWN-TNTAPKSSSEQ 62

Query: 61  --LSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVI 110
             +S++LGML  YTPAF+ G VSLW FPNEDRRI L+KSAL LHF KR+LEV+
Sbjct: 63  FKISSRLGMLFLYTPAFIAGAVSLWFFPNEDRRILLIKSALTLHFFKRDLEVL 114

[Close]

BLAST of HG10015593 vs. NCBI nr
Match: KAG6575776.1 (Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 147.1 bits (370), Expect = 9.8e-32
Identity = 79/112 (70.54%), Postives = 90/112 (80.36%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNS---S 60
           MD LSEFVF PP SLF+NVMTVV   VVA++GLSEARG+H++YSKFWN  N+AP S    
Sbjct: 1   MDALSEFVFAPPPSLFLNVMTVVIPVVVAMLGLSEARGRHLQYSKFWN-ANSAPKSLEQF 60

Query: 61  NLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVI 110
            L + LGMLLAYTPA L G+VSLW FPNEDRR  LLKSAL LHFLKR+LEV+
Sbjct: 61  KLPSWLGMLLAYTPALLAGVVSLWFFPNEDRRTLLLKSALTLHFLKRDLEVL 111

[Close]

BLAST of HG10015593 vs. ExPASy TrEMBL
Match: A0A5D3CDA5 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001760 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.3e-41
Identity = 92/114 (80.70%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNA---PNSS 60
           MD LSEFVFPPPSS FVNVMTVVSSA+VAVVGLSEARGKH++YSKFWN  NNA     S 
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNANTDRKSF 60

Query: 61  NLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           NLSAKLGMLLAY PAFLVG+VS WLFPNEDRRI +LKSAL+LHF KRNLEV+ L
Sbjct: 61  NLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFL 114

[Close]

BLAST of HG10015593 vs. ExPASy TrEMBL
Match: A0A1S3BGK3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489362 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.3e-41
Identity = 92/114 (80.70%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNA---PNSS 60
           MD LSEFVFPPPSS FVNVMTVVSSA+VAVVGLSEARGKH++YSKFWN  NNA     S 
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNANTDRKSF 60

Query: 61  NLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           NLSAKLGMLLAY PAFLVG+VS WLFPNEDRRI +LKSAL+LHF KRNLEV+ L
Sbjct: 61  NLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFL 114

[Close]

BLAST of HG10015593 vs. ExPASy TrEMBL
Match: A0A0A0KWK8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612900 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.1e-40
Identity = 90/113 (79.65%), Postives = 98/113 (86.73%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFW--NTTNNAPNSSN 60
           MDTLSEFVFPPP+S FVNVMTVVSSA+VAVVGLSEARGKH+KYSKFW  N  N    S N
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNANTDRKSFN 60

Query: 61  LSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVISL 112
           LSAKLGML+AY PAFL G+VSLWLFP+EDRRI LLKSAL+LHF KRNLEV+ L
Sbjct: 61  LSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFL 113

[Close]

BLAST of HG10015593 vs. ExPASy TrEMBL
Match: A0A6J1D974 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=LOC111018758 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.5e-33
Identity = 78/113 (69.03%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSN-- 60
           M+ LSEF+FP P SLFVNVMTVVSSAVV  +G+SEA+GKH++YSKFWN TN AP SS+  
Sbjct: 3   MNALSEFLFPAPPSLFVNVMTVVSSAVVTAIGISEAKGKHLQYSKFWN-TNTAPKSSSEQ 62

Query: 61  --LSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVI 110
             +S++LGML  YTPAF+ G VSLW FPNEDRRI L+KSAL LHF KR+LEV+
Sbjct: 63  FKISSRLGMLFLYTPAFIAGAVSLWFFPNEDRRILLIKSALTLHFFKRDLEVL 114

[Close]

BLAST of HG10015593 vs. ExPASy TrEMBL
Match: A0A6J1HUJ4 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467586 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.1e-31
Identity = 77/112 (68.75%), Postives = 90/112 (80.36%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNS---S 60
           MD LSEFVF PP SLF+NV+TVV   VVA++GLSEARG+H++YSKFWN  N+AP S    
Sbjct: 1   MDALSEFVFAPPPSLFLNVVTVVIPVVVAMLGLSEARGRHLQYSKFWN-ANSAPKSLEKF 60

Query: 61  NLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVI 110
            L + LGMLLAYTPA L G++SLW FPNEDRR  LLKSAL LHFLKR+LEV+
Sbjct: 61  KLPSWLGMLLAYTPALLAGVLSLWFFPNEDRRTLLLKSALTLHFLKRDLEVL 111

[Close]

BLAST of HG10015593 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 8.3e-21
Identity = 53/114 (46.49%), Postives = 76/114 (66.67%), Query Frame = 0


Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNS---- 60
           M+ ++ FV+PPP S+ +N M+VV  A +A +G SE RG H+KYSKF   ++++P      
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF-GVSSSSPQPQKER 60

Query: 61  -SNLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVI 110
             ++S++ GMLL YTPAFL    S ++ P++D R  LLKSAL LHF KR  EV+
Sbjct: 61  FGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVL 113

The following BLAST results are available for this feature:

BLAST of HG10015593 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892898.1	6.5e-44	86.49	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]
XP_008446724.1	1.5e-40	80.70	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo] >KAA00346...	[more]
XP_004135050.1	1.7e-39	79.65	3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KGN52161.1 hypothetic...	[more]
XP_022150685.1	3.0e-33	69.03	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Momordica charantia]	[more]
KAG6575776.1	9.8e-32	70.54	Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of HG10015593 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of HG10015593 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CDA5	7.3e-41	80.70	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BGK3	7.3e-41	80.70	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A0A0KWK8	8.1e-40	79.65	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6129...	[more]
A0A6J1D974	1.5e-33	69.03	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1HUJ4	3.1e-31	68.75	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of HG10015593 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	8.3e-21	46.49	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 7..110
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 7..110

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10015593.1	HG10015593.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors