HG10015592 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10015592
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Chr02: 27948655 .. 27949095 (-)
RNA-Seq Expression	HG10015592
Synteny	HG10015592

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCGAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAAGACTTTCCTACCCATCTTAAAGCCCTTATCCCCTTTCTATTTTAG

mRNA sequence

ATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCGAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAAGACTTTCCTACCCATCTTAAAGCCCTTATCCCCTTTCTATTTTAG

Coding sequence (CDS)

ATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCGAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAAGACTTTCCTACCCATCTTAAAGCCCTTATCCCCTTTCTATTTTAG

Protein sequence

MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF

Homology

[Close]

BLAST of HG10015592 vs. NCBI nr
Match: XP_038892898.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 261.5 bits (667), Expect = 4.2e-66
Identity = 127/146 (86.99%), Postives = 137/146 (93.84%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           MVIDTVITIS SYFSSTAIMIYTQHLSQGLP+PSIDLK IGIAL+ IGI GNFYHHYLLS
Sbjct: 118 MVIDTVITISLSYFSSTAIMIYTQHLSQGLPEPSIDLKKIGIALYLIGITGNFYHHYLLS 177

Query: 61  RARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRS 120
           +ARKQGETSYKIPKGGLFS +ICPHY+FE IE+FGFA ISQTIYS FFA ATALYL+GRS
Sbjct: 178 KARKQGETSYKIPKGGLFSFIICPHYLFEIIEFFGFAFISQTIYSLFFAIATALYLTGRS 237

Query: 121 YATRKWYVSKFEDFPTHLKALIPFLF 147
           YATRKWYVSKFEDFP+H+KAL+PFLF
Sbjct: 238 YATRKWYVSKFEDFPSHVKALLPFLF 263

[Close]

BLAST of HG10015592 vs. NCBI nr
Match: XP_004135050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KGN52161.1 hypothetical protein Csa_008531 [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 1.5e-63
Identity = 124/147 (84.35%), Postives = 136/147 (92.52%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQG-LPQPSIDLKNIGIALFSIGIIGNFYHHYLL 60
           M +D+VITISFSYFSSTAIMIYTQHLSQG L +P IDLK+IG+ LF IGIIGNFYHHYLL
Sbjct: 120 MAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYLL 179

Query: 61  SRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           S+ RKQGETSYKIPKGGLFSL+ICPHY+FE IE+FGFA ISQTIYS FFAFATALYLSGR
Sbjct: 180 SQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSGR 239

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATRKWYVSKFEDFP H+KAL+PF+F
Sbjct: 240 SYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]

BLAST of HG10015592 vs. NCBI nr
Match: XP_008446724.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo] >KAA0034633.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa] >TYK09184.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 243.4 bits (620), Expect = 1.2e-60
Identity = 120/147 (81.63%), Postives = 132/147 (89.80%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQG-LPQPSIDLKNIGIALFSIGIIGNFYHHYLL 60
           M I +VITISFSYFSSTAIMIYTQHLSQG LP+P IDLK IG+ LF IGI+GNFYHHYLL
Sbjct: 121 MAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHYLL 180

Query: 61  SRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           S  RK GETSYKIP+GGLF+ VICPHY+FE IE+FGFA ISQTIYS FFAFATALYL+GR
Sbjct: 181 SGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLTGR 240

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATRKWYVSKFEDFP H+KAL+PF+F
Sbjct: 241 SYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]

BLAST of HG10015592 vs. NCBI nr
Match: XP_023542215.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.8 bits (608), Expect = 2.9e-59
Identity = 114/146 (78.08%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           MV DT ITIS SYFSSTA+MIYTQHLS+ LP+PSIDLKNIGIALF IGIIGNFYHH+LLS
Sbjct: 120 MVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLLS 179

Query: 61  RARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRS 120
           + RKQGET YKIP+GGLF+ VICPHY+FE IEYFGFALISQT +S  FAF TA YL+GRS
Sbjct: 180 KTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFALISQTFFSLSFAFGTAFYLAGRS 239

Query: 121 YATRKWYVSKFEDFPTHLKALIPFLF 147
            AT++WY SKFEDFP H+KAL+PF+F
Sbjct: 240 SATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of HG10015592 vs. NCBI nr
Match: KAG6573901.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012967.1 3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.3 bits (604), Expect = 8.5e-59
Identity = 113/146 (77.40%), Postives = 128/146 (87.67%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           MV DT ITIS SYFSSTA+MIYTQHLS+ LP+PSIDLKNIGIALF IGIIGNFYHH+LLS
Sbjct: 120 MVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLLS 179

Query: 61  RARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRS 120
           + RKQGET YKIP+GGLF+ VICPHY+FE IEYFGFA ISQT +S  FAF TA YL+GRS
Sbjct: 180 KTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGRS 239

Query: 121 YATRKWYVSKFEDFPTHLKALIPFLF 147
            AT++WY SKFEDFP H+KAL+PF+F
Sbjct: 240 SATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of HG10015592 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.0e-19
Identity = 47/107 (43.93%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 40  IGIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALI 99
           IG  L+  G++ N +  ++L   RK G+T YKIP+GGLF  V   +Y  E +E+ G+AL 
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 100 SQTIYSFFFAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           S ++    FAF T  +LSGR+    +WY+ KFE++P   K LIPFLF
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of HG10015592 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-18
Identity = 46/107 (42.99%), Postives = 67/107 (62.62%), Query Frame = 0


Query: 40  IGIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALI 99
           IG  L+  G++ N +  ++L   RK G+T YKIP+GGLF  V   +Y  E +E+ G+AL 
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 100 SQTIYSFFFAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           S ++    FAF T  +LSGR+    +WY+ KFE++P   K +IPFLF
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of HG10015592 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-17
Identity = 46/105 (43.81%), Postives = 63/105 (60.00%), Query Frame = 0


Query: 41  GIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALIS 100
           G AL+ +G++ N +  ++L   RK GET YKIP+GGLF  V   +Y  E +E+ GFAL S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209

Query: 101 QTIYSFFFAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFL 146
            ++    FA  T   L  R+    +WY+ KFED+P   K LIPFL
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of HG10015592 vs. ExPASy Swiss-Prot
Match: A5PJS2 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.8e-17
Identity = 51/133 (38.35%), Postives = 70/133 (52.63%), Query Frame = 0


Query: 13  YFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSRARKQGETSYKI 72
           Y  S  +  Y  +    L  P       G AL+ IG++ N +  ++L   RK GET YKI
Sbjct: 127 YLQSRYLSQYAVYADDWLSDPRF---LTGSALWLIGMLINIHSDHVLRNLRKPGETGYKI 186

Query: 73  PKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATRKWYVSKFE 132
           P+GGLF  +   +Y  E +E+ G+AL S +I  + FA  T   L  R+    KWY  KFE
Sbjct: 187 PRGGLFEYISAANYFGEVVEWCGYALASWSIQGWAFAVFTFCVLFTRAQQHHKWYHEKFE 246

Query: 133 DFPTHLKALIPFL 146
           D+P   K +IPFL
Sbjct: 247 DYPKFRKIMIPFL 256

[Close]

BLAST of HG10015592 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 4.9e-17
Identity = 43/107 (40.19%), Postives = 71/107 (66.36%), Query Frame = 0


Query: 40  IGIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALI 99
           +G+ LF +G+  N +  Y+L + RK GE +Y+IPKGGLF+ V   +++ E IE+ G+AL 
Sbjct: 148 LGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALA 207

Query: 100 SQTIYSFFFAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           + ++ +  FAF +  +L  R++   ++Y+  FED+P   KALIPF+F
Sbjct: 208 TWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of HG10015592 vs. ExPASy TrEMBL
Match: A0A0A0KWK8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612900 PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.3e-64
Identity = 124/147 (84.35%), Postives = 136/147 (92.52%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQG-LPQPSIDLKNIGIALFSIGIIGNFYHHYLL 60
           M +D+VITISFSYFSSTAIMIYTQHLSQG L +P IDLK+IG+ LF IGIIGNFYHHYLL
Sbjct: 120 MAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHYLL 179

Query: 61  SRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           S+ RKQGETSYKIPKGGLFSL+ICPHY+FE IE+FGFA ISQTIYS FFAFATALYLSGR
Sbjct: 180 SQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLSGR 239

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATRKWYVSKFEDFP H+KAL+PF+F
Sbjct: 240 SYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]

BLAST of HG10015592 vs. ExPASy TrEMBL
Match: A0A5D3CDA5 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001760 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.8e-61
Identity = 120/147 (81.63%), Postives = 132/147 (89.80%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQG-LPQPSIDLKNIGIALFSIGIIGNFYHHYLL 60
           M I +VITISFSYFSSTAIMIYTQHLSQG LP+P IDLK IG+ LF IGI+GNFYHHYLL
Sbjct: 121 MAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHYLL 180

Query: 61  SRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           S  RK GETSYKIP+GGLF+ VICPHY+FE IE+FGFA ISQTIYS FFAFATALYL+GR
Sbjct: 181 SGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLTGR 240

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATRKWYVSKFEDFP H+KAL+PF+F
Sbjct: 241 SYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]

BLAST of HG10015592 vs. ExPASy TrEMBL
Match: A0A1S3BGK3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489362 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.8e-61
Identity = 120/147 (81.63%), Postives = 132/147 (89.80%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQG-LPQPSIDLKNIGIALFSIGIIGNFYHHYLL 60
           M I +VITISFSYFSSTAIMIYTQHLSQG LP+P IDLK IG+ LF IGI+GNFYHHYLL
Sbjct: 121 MAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHYLL 180

Query: 61  SRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           S  RK GETSYKIP+GGLF+ VICPHY+FE IE+FGFA ISQTIYS FFAFATALYL+GR
Sbjct: 181 SGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLTGR 240

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATRKWYVSKFEDFP H+KAL+PF+F
Sbjct: 241 SYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]

BLAST of HG10015592 vs. ExPASy TrEMBL
Match: A0A6J1G134 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111449728 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.1e-59
Identity = 113/146 (77.40%), Postives = 128/146 (87.67%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           MV DT ITIS SYFSSTA+MIYTQHLS+ LP+PSIDLKNIGIALF IGIIGNFYHH+LLS
Sbjct: 120 MVFDTAITISSSYFSSTALMIYTQHLSKALPEPSIDLKNIGIALFLIGIIGNFYHHFLLS 179

Query: 61  RARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRS 120
           + RKQGET YKIP+GGLF+ VICPHY+FE IEYFGFA ISQT +S  FAF TA YL+GRS
Sbjct: 180 KTRKQGETGYKIPQGGLFNTVICPHYLFEIIEYFGFAFISQTFFSLSFAFGTAFYLAGRS 239

Query: 121 YATRKWYVSKFEDFPTHLKALIPFLF 147
            AT++WY SKFEDFP H+KAL+PF+F
Sbjct: 240 SATKRWYASKFEDFPNHIKALLPFVF 265

[Close]

BLAST of HG10015592 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 2.7e-58
Identity = 113/146 (77.40%), Postives = 127/146 (86.99%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           MV DT I ISFSY  ST IMIYTQHLSQGLP+P IDLKNIGIALF IGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 61  RARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRS 120
           + RK GET YKIPKGGLFSLVICPHY+FE IE+FGFA ISQ +Y   F+  TA+YL+GRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 121 YATRKWYVSKFEDFPTHLKALIPFLF 147
           YATRKWYVSKFE+FP+H+KAL+PF+F
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of HG10015592 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 197.2 bits (500), Expect = 9.1e-51
Identity = 92/147 (62.59%), Postives = 119/147 (80.95%), Query Frame = 0


Query: 1   MVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLS 60
           M ID+ +TIS SYFSSTA+M+Y+Q+L+ GL +PS D+K  G+ +F +GI+GN YHH LL+
Sbjct: 122 MAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLA 181

Query: 61  RARKQ-GETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGR 120
           + RK+ G+  YKIPKGGLF ++ICPHY+FE + ++ F LISQTIYSF FA  T LYL GR
Sbjct: 182 KLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGR 241

Query: 121 SYATRKWYVSKFEDFPTHLKALIPFLF 147
           SYATR WY+SKF+DFP H+KALIPF+F
Sbjct: 242 SYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of HG10015592 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.0e-14
Identity = 41/108 (37.96%), Postives = 63/108 (58.33%), Query Frame = 0


Query: 40  IGIALFSIGIIGNFYHHYLLSRARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALI 99
           IG+ +F  G+  N      L R +K+    Y IP+GG F LV CP+Y  E IE+ G+A++
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 100 SQTIYSFFFAFATALYLSGRSYATRKWYVSKF-EDFPTHLKALIPFLF 147
           + +     F   T   L  R+ A+ KWY++KF E++P   KA+IPF++
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of HG10015592 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 7.3e-08
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 37  LKNIGIALFSIGIIGNFYHHYLLSRARKQGETS--YKIPKGGLFSLVICPHYMFETIEYF 96
           L+ IG A+F  G I     H +L   R+    +  Y IP G  F +V CPH++ E + Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 97  GFALISQ----TIYSFF-FAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           G  + S     +I+  F F  A   Y +G    T +WY+ KFE++P    A+ P ++
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of HG10015592 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 9.5e-08
Identity = 38/117 (32.48%), Postives = 59/117 (50.43%), Query Frame = 0


Query: 37  LKNIGIALFSIGIIGNFYHHYLLSRARKQGETS--YKIPKGGLFSLVICPHYMFETIEYF 96
           L+ IG A+F  G I     H +L   R+    +  Y IP G  F +V  PH++ E + Y 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 97  GFALISQ----TIYSFF-FAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           G  + S     TI+  F F  A   Y +G    T +WY+ KFE++P +  A+ P+++
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

[Close]

BLAST of HG10015592 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 9.5e-08
Identity = 38/117 (32.48%), Postives = 59/117 (50.43%), Query Frame = 0


Query: 37  LKNIGIALFSIGIIGNFYHHYLLSRARKQGETS--YKIPKGGLFSLVICPHYMFETIEYF 96
           L+ IG A+F  G I     H +L   R+    +  Y IP G  F +V  PH++ E + Y 
Sbjct: 229 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 288

Query: 97  GFALISQ----TIYSFF-FAFATALYLSGRSYATRKWYVSKFEDFPTHLKALIPFLF 147
           G  + S     TI+  F F  A   Y +G    T +WY+ KFE++P +  A+ P+++
Sbjct: 289 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of HG10015592 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892898.1	4.2e-66	86.99	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]
XP_004135050.1	1.5e-63	84.35	3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KGN52161.1 hypothetic...	[more]
XP_008446724.1	1.2e-60	81.63	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo] >KAA00346...	[more]
XP_023542215.1	2.9e-59	78.08	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6573901.1	8.5e-59	77.40	3-oxo-5-alpha-steroid 4-dehydrogenase 1, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of HG10015592 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	4.0e-19	43.93	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	1.2e-18	42.99	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q68FF9	1.7e-17	43.81	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
A5PJS2	3.8e-17	38.35	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Bos taurus OX=9913 GN=SRD5A1 PE=2 SV=...	[more]
Q28892	4.9e-17	40.19	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2...	[more]

BLAST of HG10015592 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWK8	7.3e-64	84.35	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6129...	[more]
A0A5D3CDA5	5.8e-61	81.63	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BGK3	5.8e-61	81.63	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1G134	4.1e-59	77.40	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1GZA3	2.7e-58	77.40	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of HG10015592 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	9.1e-51	62.59	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	4.0e-14	37.96	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	7.3e-08	32.48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	9.5e-08	32.48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	9.5e-08	32.48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 36..146 e-value: 1.7E-30 score: 106.0
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 41..121 score: 26.237989
None	No IPR available	GENE3D	1.20.120.1630		coord: 2..145 e-value: 2.0E-5 score: 26.4
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 1..146
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 1..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10015592.1	HG10015592.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors