HG10015590 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015590 vs. NCBI nr
Match: XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 489.2 bits (1258), Expect = 2.3e-134
Identity = 244/266 (91.73%), Postives = 254/266 (95.49%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           MDVLSAFLFPPPPS FVN+MTVVSSTA+A+AGISEINGKHLQYSKFWNANT PK SSE +
Sbjct: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
            LSSKYGMLLAYTPAFLAGAASFWLFPS +ERILLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MVL TAI+IS SYFLSTVIMIYTQHLSQG  EPPI+LKNIGIGLFLIGIIGNFYHHYLLS
Sbjct: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK+GETSY+IPKGGLFS VICPHYLFEIIEFFGFA ISQELYPLCFSIGTS+YLAGRS
Sbjct: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFENFPNHVKALLPFVF
Sbjct: 274 YATRKWYISKFENFPNHVKALLPFVF 299

BLAST of HG10015590 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 476.1 bits (1224), Expect = 2.0e-130
Identity = 236/266 (88.72%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           M +LSAFLFPPPPSTFVNVMTVVSSTAM +AGISEINGKHLQYSKFWNAN+ PK  SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS  ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MV +TAIKIS SY LSTVIMIYTQHLSQG  EPPIDLKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK GET YKIPKGGLFSLVICPHYLFEIIEFFGFA ISQELYPLCFS GT++YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

BLAST of HG10015590 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 476.1 bits (1224), Expect = 2.0e-130
Identity = 236/266 (88.72%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           M +LSAFLFPPPPSTFVNVMTVVSSTAM +AGISEINGKHLQYSKFWNAN+ PK  SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS  ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MV +TAIKIS SY LSTVIMIYTQHLSQG  EPPIDLKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK GET YKIPKGGLFSLVICPHYLFEIIEFFGFA ISQELYPLCFS GT++YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

BLAST of HG10015590 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 475.3 bits (1222), Expect = 3.4e-130
Identity = 236/266 (88.72%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           M +LSAFLFPPP STFVNVMTVVSSTAM +AGISEINGKHLQYSKFWNAN+ PK  SEET
Sbjct: 300 MGILSAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEET 359

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS  ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 360 KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 419

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MV +TAIKIS SY LSTVIMIYTQHLSQG  EPPIDLKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 420 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 479

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK GET YKIPKGGLFSLVICPHYLFEIIEFFGFA ISQELYPLCFS GT++YLAGRS
Sbjct: 480 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 539

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 540 YATRKWYVSKFENFPSHVKALLPFVF 565

BLAST of HG10015590 vs. NCBI nr
Match: XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])

HSP 1 Score: 474.6 bits (1220), Expect = 5.8e-130
Identity = 232/266 (87.22%), Postives = 253/266 (95.11%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           MDVLSAFLFPPPPSTFVNVMTVVS+TA+A+AG+SEINGKHLQYSKFWNAN   K S+EE+
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSG+ER+ LLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MVL+TAI+ISLSYF+ST IMIYTQHLSQG  EPPIDLKNIG+G FLIGIIGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           +TRKQG+T+YKIPKGGLFS V+CPHYLFEII +FGFA ISQELYPLCFSIGT++YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 286

BLAST of HG10015590 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.3e-19
Identity = 45/107 (42.06%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 160 IGIGLFLIGIIGNFYHHYLLSKTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALI 219
           IG GL+L G++ N +  ++L   RK G+T YKIP+GGLF  V   +Y  EI+E+ G+AL 
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 220 SQELYPLCFSIGTSLYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

BLAST of HG10015590 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.6e-19
Identity = 44/107 (41.12%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 160 IGIGLFLIGIIGNFYHHYLLSKTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALI 219
           IG GL+L G++ N +  ++L   RK G+T YKIP+GGLF  V   +Y  EI+E+ G+AL 
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 220 SQELYPLCFSIGTSLYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

BLAST of HG10015590 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 61/199 (30.65%), Postives = 104/199 (52.26%), Query Frame = 0

Query: 82  SFWLFPSGEE----RILLLKSALTLHFFKRIFEVLFIHKYSSKMVLETAIKISLSYFLST 141
           +F+LFPSG+     +  LL S    H+F R   VL+  + +          +S+++    
Sbjct: 62  TFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVAFMAFG 121

Query: 142 VIMIYTQHLSQGFSEPPIDLKN---------IGIGLFLIGIIGNFYHHYLLSKTRKQGET 201
             ++     ++  S    D +N          G+ +F++G+  N     +L   +KQG+ 
Sbjct: 122 FNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGDG 181

Query: 202 SYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRSYATRKWYI 261
            YKIP+GGLF LV CP+Y  EI+E+FG+A+++       F + T   L  R+ ATR WY+
Sbjct: 182 GYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWYL 241

Query: 262 SKF-ENFPNHVKALLPFVF 267
            KF +++P   KA++PF++
Sbjct: 242 EKFKDDYPKDRKAVIPFIY 258

BLAST of HG10015590 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.4e-17
Identity = 66/220 (30.00%), Postives = 116/220 (52.73%), Query Frame = 0

Query: 54  KPSSEETKLSSKYGMLLAYTPAFLAGAASFWLFP---SGEERILLLKSALTLHFFKRIFE 113
           KP++  T+L ++    L   P+F   A      P    G    +LL     +H+F R F 
Sbjct: 41  KPAA--TRLPARAAWFLQELPSFAVPAGILARQPLSLFGPPGTVLL-GLFCVHYFHRTFV 100

Query: 114 VLFIHK---YSSKMVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLK-NIGIGLFL 173
              +++   Y + ++       + + FL +  +IY      G+     D++  +G+ LF+
Sbjct: 101 YSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGWY---TDIRFCLGVFLFI 160

Query: 174 IGIIGNFYHHYLLSKTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPL 233
           +G+  N +  Y+L + RK GE +Y+IPKGGLF+ V   ++L EIIE+ G+AL +  L  L
Sbjct: 161 LGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALATWSLPAL 220

Query: 234 CFSIGTSLYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
            F+  +  +L  R++   ++Y+  FE++P   KAL+PF+F
Sbjct: 221 AFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

BLAST of HG10015590 vs. ExPASy Swiss-Prot
Match: P31213 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-17
Identity = 72/250 (28.80%), Postives = 128/250 (51.20%), Query Frame = 0

Query: 24  SSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEETKLSSKYGMLLAYTPAFLAGAASF 83
           S+T +A+  ++    K   Y K   +    KP++  T+L ++    L   P+F   A   
Sbjct: 14  SATLVALGALALYVAKPSGYGKHTES---LKPAA--TRLPARAAWFLQELPSFAVPAGIL 73

Query: 84  WLFP---SGEERILLLKSALTLHFFKRIFEVLFIHK---YSSKMVLETAIKISLSYFLST 143
              P    G    +LL     +H+F R F    +++   Y + ++L      + +  L  
Sbjct: 74  ARQPLSLFGPPGTVLL-GLFCVHYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQG 133

Query: 144 VIMIYTQHLSQGFSEPPIDLK-NIGIGLFLIGIIGNFYHHYLLSKTRKQGETSYKIPKGG 203
             +IY      G+     D++ ++G+ LF++G+  N +  Y+L + RK GE SY+IP+GG
Sbjct: 134 YYLIYCAEYPDGWY---TDIRFSLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGG 193

Query: 204 LFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRSYATRKWYISKFENFPN 263
           LF+ V   ++L EIIE+ G+AL +  L  L F+  +  +L  R++   ++Y+  FE++P 
Sbjct: 194 LFTYVSGANFLGEIIEWIGYALATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPK 253

Query: 264 HVKALLPFVF 267
             KAL+PF+F
Sbjct: 254 SRKALIPFIF 254

BLAST of HG10015590 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 9.6e-131
Identity = 236/266 (88.72%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           M +LSAFLFPPPPSTFVNVMTVVSSTAM +AGISEINGKHLQYSKFWNAN+ PK  SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS  ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MV +TAIKIS SY LSTVIMIYTQHLSQG  EPPIDLKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK GET YKIPKGGLFSLVICPHYLFEIIEFFGFA ISQELYPLCFS GT++YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

BLAST of HG10015590 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 9.6e-131
Identity = 236/266 (88.72%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           M +LSAFLFPPPPSTFVNVMTVVSSTAM +AGISEINGKHLQYSKFWNAN+ PK  SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS  ERIL+LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MV +TAIKIS SY LSTVIMIYTQHLSQG  EPPIDLKNIGI LFLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           KTRK GET YKIPKGGLFSLVICPHYLFEIIEFFGFA ISQELYPLCFS GT++YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

BLAST of HG10015590 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.2e-130
Identity = 232/266 (87.22%), Postives = 250/266 (93.98%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           MD+LSAFLFPPPPSTFVNVMTVVSST++A+AG+SEI GKHLQYSKFWNAN+  K S++E+
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLL YTPAFLAGAASFWLFPSG+ER  LLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MVL+TAI+IS SYFLST IMIYTQHLSQG  EPPIDLKNIG+GLFL GIIGNFYHHYLLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           +TRKQ ET+YKIPKGGLFS VICPHYLFEII +FGFA ISQELYPLCFSIGT++YLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFENFPNHVKALLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276

BLAST of HG10015590 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 3.3e-123
Identity = 220/266 (82.71%), Postives = 241/266 (90.60%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           MD+ SAFLFPPPPSTFVNVMTVVSSTAMA++G SE+NGKHLQYSKFWNAN   K SSEE+
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS + RILLLKSALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MVL+TA KISLSYFLST IMIYTQHLSQGF  P IDLKNIGIG F++GIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           +TR +GE  YKIP GGLF LVICPHYLFEII +FGFA ISQ ++PLCF+ GT+LYLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

BLAST of HG10015590 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.5e-118
Identity = 216/266 (81.20%), Postives = 236/266 (88.72%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEET 60
           MDVLSAFLFPPPPSTFVNVMTVVS+TA+A+AG+SEINGKHLQYSKFWNAN   K S+EE+
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSG+ER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLLS 180
           MVL+TAI+ISLSYF+ST IMIYTQHLSQG  EPPIDLKNIG+G FLIGIIGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 KTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAGRS 240
           +TRKQG+T+YKIPKGGLFS V+CPHYLFEII +FGFA ISQELYPLCFSIGT++YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 268

BLAST of HG10015590 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 321.2 bits (822), Expect = 7.6e-88
Identity = 155/268 (57.84%), Postives = 208/268 (77.61%), Query Frame = 0

Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTAMAVAGISEINGKHLQYSKFWNANTVPKPSSEE- 60
           M+++++F++PPPPS  +N M+VV   A+A  G SEI G HL+YSKF  +++ P+P  E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  TKLSSKYGMLLAYTPAFLAGAASFWLFPSGEERILLLKSALTLHFFKRIFEVLFIHKYSS 120
             +SS+ GMLL YTPAFLA A+SF++ PS + R LLLKSAL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVLETAIKISLSYFLSTVIMIYTQHLSQGFSEPPIDLKNIGIGLFLIGIIGNFYHHYLL 180
            M +++A+ IS SYF ST +M+Y+Q+L+ G +EP  D+K  G+ +F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SKTRKQ-GETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAG 240
           +K RK+ G+  YKIPKGGLF ++ICPHYLFEI+ F+ F LISQ +Y   F++GT LYL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSYATRKWYISKFENFPNHVKALLPFVF 267
           RSYATR WY+SKF++FP H+KAL+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

BLAST of HG10015590 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-13
Identity = 60/209 (28.71%), Postives = 99/209 (47.37%), Query Frame = 0

Query: 76  FLAGAASFW----LFPSGEERI----LLLKSALTLHFFKRIFEVLFIHKYSSKMVLETAI 135
           F+  + + W    LFP G   +    LLL S   +H+F R          SS    +   
Sbjct: 54  FVMESPTLWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGF 113

Query: 136 KISLSYFLSTVIMIYTQHLSQGFSEPPIDLKN---------IGIGLFLIGIIGNFYHHYL 195
            I+++    T  ++     ++  S    D ++         IG+ +F+ G+  N      
Sbjct: 114 PITIAALAFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRT 173

Query: 196 LSKTRKQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFALISQELYPLCFSIGTSLYLAG 255
           L + +K+    Y IP+GG F LV CP+Y  E IE+ G+A+++     + F + T   L  
Sbjct: 174 LVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFP 233

Query: 256 RSYATRKWYISKF-ENFPNHVKALLPFVF 267
           R+ A+ KWYI+KF E +P   KA++PFV+
Sbjct: 234 RARASHKWYIAKFKEEYPKTRKAVIPFVY 262

BLAST of HG10015590 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-11
Identity = 48/152 (31.58%), Postives = 73/152 (48.03%), Query Frame = 0

Query: 103 HFFKRIFEVLFIHKYSSKMVLETAIKISLSYFLS--TVIMIYTQHLSQGFSEPPIDLK-N 162
           H+FKRI E  F+H++S        +  + +Y+ S    I  Y  H       P  DL+  
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNH---PLYTPVSDLQMK 200

Query: 163 IGIGLFLIGIIGNFYHHYLLSKTR-KQGETSYKIPKGGLFSLVICPHYLFEIIEFFGFAL 222
           IG G  L+  + NFY H LL   R   G   Y+IP+G LF++V C +Y  EI ++ GF +
Sbjct: 201 IGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNI 260

Query: 223 ISQELYPLCFSIGTSLYLAGRSYATRKWYISK 251
            +Q       +I   ++LA  +     W + K
Sbjct: 261 ATQ-------TIAGYVFLAVAALIMTNWALGK 282

BLAST of HG10015590 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.4e-09
Identity = 40/115 (34.78%), Postives = 57/115 (49.57%), Query Frame = 0

Query: 157 LKNIGIGLFLIGIIGNFYHHYLLSKTRKQGETS--YKIPKGGLFSLVICPHYLFEIIEFF 216
           L+ IG  +FL G I     H +L   R+    +  Y IP G  F +V CPH+L EI+ + 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 217 GFALISQELYPLCFSIGTSLYLAGRSYA---TRKWYISKFENFPNHVKALLPFVF 267
           G  LIS     +   +      A  +YA   T +WY+ KFEN+P    A+ P V+
Sbjct: 267 GL-LISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

BLAST of HG10015590 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 3.5e-08
Identity = 39/117 (33.33%), Postives = 60/117 (51.28%), Query Frame = 0

Query: 157 LKNIGIGLFLIGIIGNFYHHYLLSKTRKQGETS--YKIPKGGLFSLVICPHYLFEIIEFF 216
           L+ IG  +FL G I     H +L   R+    +  Y IP G  F +V  PH+L EI+ + 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 217 GFALISQ----ELYPLCFSIGTSL-YLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           G  + S      ++ L   +  +L Y AG    T +WY+ KFEN+P +  A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343