HG10015226 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10015226
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr02: 24981520 .. 24983722 (+)
RNA-Seq ExpressionHG10015226
SyntenyHG10015226
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAAATTCTTTCTGCTTTCGCTTCTCGGTATGTTGATCATTGCCTATGGCGTTTCAGGTAGTATCGACATTGACATGAGTAAAGAACTTGAAAGTTTTGACATTGAAGAAGAGGACGACAATGCGATGGACTCGTACGCAGTCCCGGTGTGGAGAAGTGAGCGTGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGATGCGGTTGGAGATGGAGTTACAGATGATACTATGGTACATGGATATTTCTGTTTGGAAATATGGTTTTTGCAGATATAATACTGAACCAAAGTGTTTATTTTGCAATCAGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCACTTCAAAATCAGTTCTTTTAGTGCCAAAAGAAAGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTAATCCAGGTAAGCCACAGGATTATAAGGATTTTTTTATCCCAATTAAAGGTGTGAAGATCATGGTTGAAAGATAGAGCTAACTTTGCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGCTATGGGATCCAAAGTATCCACGACAATGGCTGGAATTTACAAAACTAAATGCAGCTACCTTTCAAGGAGATGGAGTTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAGAACAAGTCCAGGGTAAAAATTTTAACTATTTCTGTGCTTGTTTCTTAGGCTATGTTATTGAAATGTTAATCATTTCTGGTTTTCTGGTTTTTTTTTTTTTTTTCCTTTTTTTCCCCAGCCTTGCAAAGGAGCACCAACAGTAAGGACAAACTACTCTCCTCTTAATGAAAATCCTGATTTTTCCATGTGTAGCACATTCTGATTTAAGGGATTTCTTGCAGGCATTAACCATTGACTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTGAGACTGTTCGAATTACAGAGGTTAGAGTCTCAGCTCCAGGAGATAGCCCGAATACCGACGGGATCCACATCACTCAATCAACAAATGTTGTAGTTCAAAACTGCAAGATTTCGACAGGTTTTCATTCTGACTTCATCTTGTGATCTTTAACACAACTTTACCATCTGAACCGGCTGAAAAGAGTATTGATTGATAATGGTTCTGCAATTTATATGCAGGTGATGATTGCATCTCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAGGGAATATCGTGTGGACCAGGACACGGGATCAGGTATCTGCAAATGTTTTCATTTCACTCGGCACTGTTCGCTGTTCGATTTATTTCAAGCAACAAAACCAGATCAATGTCTTAGAATTCCTTTTCTTTGTTTGTTTCTAATGAAATTGTGTTCCTTAGCATTGGAAGTTTAGGGAAAGACAACTCCACCGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACGACAAATGGTGTTAGAATCAAGACTTGGCAGGTTAGAATCACAGCTTTAACCACCATTTTGATACTTACTTTTACATGGTTGATTATAACCAAACACAAAGGGAAAATTTCTCTATTCATGTGGTGCAGGGAGGTTCCGGATATGTTCGTTCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATTATCGATCAGTTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGGTATTATGCTCCCTCAAAGTCAAGAATTTAGCTTTTAATCAAACAGTAACATTAAAAATAAGATCATATTGAGACAGAAAGACAAAGTTTGTCCATTATCCATCATTGAGTTCAATGCCTTTTGTTTCTGTCAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAATTCGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTTTAAGCAATGTCAACTTGGAGAAAACGGATGGAACAGTGGAGACCTATTGCCACGCCGCTCAAGGCTTTGGATATGGCGTTGTGCACCCGTCCGCAGACTGCTTAACTTCCAGCGACAATGACTTCACAGCTTTAGATCAGACCCAAGTTCCGGAGCTGGAGGCAATGCAACTCCCTACTGAACAGCACATAGTTCACACTGAGCTCTGA

mRNA sequence

ATGGATAAATTCTTTCTGCTTTCGCTTCTCGGTATGTTGATCATTGCCTATGGCGTTTCAGGTAGTATCGACATTGACATGAGTAAAGAACTTGAAAGTTTTGACATTGAAGAAGAGGACGACAATGCGATGGACTCGTACGCAGTCCCGGTGTGGAGAAGTGAGCGTGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGATGCGGTTGGAGATGGAGTTACAGATGATACTATGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCACTTCAAAATCAGTTCTTTTAGTGCCAAAAGAAAGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTAATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGCTATGGGATCCAAAGTATCCACGACAATGGCTGGAATTTACAAAACTAAATGCAGCTACCTTTCAAGGAGATGGAGTTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAGAACAAGTCCAGGGCATTAACCATTGACTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTGAGACTGTTCGAATTACAGAGGTTAGAGTCTCAGCTCCAGGAGATAGCCCGAATACCGACGGGATCCACATCACTCAATCAACAAATGTTGTAGTTCAAAACTGCAAGATTTCGACAGGTGATGATTGCATCTCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAGGGAATATCGTGTGGACCAGGACACGGGATCAGCATTGGAAGTTTAGGGAAAGACAACTCCACCGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACGACAAATGGTGTTAGAATCAAGACTTGGCAGGGAGGTTCCGGATATGTTCGTTCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATTATCGATCAGTTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAATTCGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTTTAAGCAATGTCAACTTGGAGAAAACGGATGGAACAGTGGAGACCTATTGCCACGCCGCTCAAGGCTTTGGATATGGCGTTGTGCACCCGTCCGCAGACTGCTTAACTTCCAGCGACAATGACTTCACAGCTTTAGATCAGACCCAAGTTCCGGAGCTGGAGGCAATGCAACTCCCTACTGAACAGCACATAGTTCACACTGAGCTCTGA

Coding sequence (CDS)

ATGGATAAATTCTTTCTGCTTTCGCTTCTCGGTATGTTGATCATTGCCTATGGCGTTTCAGGTAGTATCGACATTGACATGAGTAAAGAACTTGAAAGTTTTGACATTGAAGAAGAGGACGACAATGCGATGGACTCGTACGCAGTCCCGGTGTGGAGAAGTGAGCGTGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCGATGCGGTTGGAGATGGAGTTACAGATGATACTATGGCTTTCAGGAAGGCATGGGAAACTGCCTGCAGCACTTCAAAATCAGTTCTTTTAGTGCCAAAAGAAAGGAGATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGCAAAGATGGTATGGTAATCCAGATTGAAGGAACAATAGTTGCACCAGATGAACCAGAGCTATGGGATCCAAAGTATCCACGACAATGGCTGGAATTTACAAAACTAAATGCAGCTACCTTTCAAGGAGATGGAGTTATCGACGGCTCCGGTGAGAAATGGTGGGCAGAATCCTGCAAAAAGAACAAGTCCAGGGCATTAACCATTGACTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCATCATTGCGCGGTCTGAGACTGTTCGAATTACAGAGGTTAGAGTCTCAGCTCCAGGAGATAGCCCGAATACCGACGGGATCCACATCACTCAATCAACAAATGTTGTAGTTCAAAACTGCAAGATTTCGACAGGTGATGATTGCATCTCCATTGTCAATGCTAGCTCTGGCATCAAAATGAAGGGAATATCGTGTGGACCAGGACACGGGATCAGCATTGGAAGTTTAGGGAAAGACAACTCCACCGGCATTGTGACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACGACAAATGGTGTTAGAATCAAGACTTGGCAGGGAGGTTCCGGATATGTTCGTTCCGTACGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATTATTATCGATCAGTTCTACTGTGACTCTCCTACTACTTGTGAAACTCAGACATCTGCCGTGAAAATAAGCCAAATCATGTATCGGAACATAAGTGGAACCACAACGAGCAAGAACGCGATGAAATTCGCCTGTAGCGACAGCGTTCCATGCAGCAACATAATTTTAAGCAATGTCAACTTGGAGAAAACGGATGGAACAGTGGAGACCTATTGCCACGCCGCTCAAGGCTTTGGATATGGCGTTGTGCACCCGTCCGCAGACTGCTTAACTTCCAGCGACAATGACTTCACAGCTTTAGATCAGACCCAAGTTCCGGAGCTGGAGGCAATGCAACTCCCTACTGAACAGCACATAGTTCACACTGAGCTCTGA

Protein sequence

MDKFFLLSLLGMLIIAYGVSGSIDIDMSKELESFDIEEEDDNAMDSYAVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL
Homology
BLAST of HG10015226 vs. NCBI nr
Match: XP_008446196.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 434/469 (92.54%), Postives = 445/469 (94.88%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDK FLLSLLG+LIIAYGVSG+     DIDM +ELESFDIEEEDD+ + SY VP WRSER
Sbjct: 38  MDKLFLLSLLGLLIIAYGVSGTFVSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSER 97

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GSK+LMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKE+RYLVNATTFKGPCK
Sbjct: 98  GSKILMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCK 157

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMVIQIEGTIVAPDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 158 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       A TIDSSSNIRVKGLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTD
Sbjct: 218 KSRPCKGAPTAFTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTD 277

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVVQN KISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV
Sbjct: 278 GIHITQSTNVVVQNSKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 337

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE
Sbjct: 338 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 397

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 398 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 457

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYG+VHPSADCLTSSDN+F ALDQTQV ELE +QLPTEQHIVHTEL
Sbjct: 458 QGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIVHTEL 506

BLAST of HG10015226 vs. NCBI nr
Match: XP_011655619.1 (probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus])

HSP 1 Score: 853.2 bits (2203), Expect = 1.0e-243
Identity = 429/469 (91.47%), Postives = 443/469 (94.46%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDK FLLSLLG+ IIAYGVSG+     DIDM KELESFDIEEEDD+ + SY VPVWRSER
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GSK+LMNVDSFDA+GDGVTDDTMAFRKAWETACSTSKSVLLVPKE+ YLVNATTFKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMVIQIEGTIVAPDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       ALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRIT VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           TKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENPIIIDQFYCDSPTTCE
Sbjct: 301 TKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE 360

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           +QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 SQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYGVVHPSADCLTSSDN+F + DQTQV ELE +QL TEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL 469

BLAST of HG10015226 vs. NCBI nr
Match: TYK15614.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 838.6 bits (2165), Expect = 2.6e-239
Identity = 421/450 (93.56%), Postives = 430/450 (95.56%), Query Frame = 0

Query: 9   LLGMLIIAYGVSGSIDIDMSKELESFDIEEEDDNAMDSYAVPVWRSERGSKVLMNVDSFD 68
           LL   II   VS S DIDM +ELESFDIEEEDD+ + SY VP WRSERGSK+LMNVDSFD
Sbjct: 143 LLVNSIIGTFVSDS-DIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFD 202

Query: 69  AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGMVIQIEGTIV 128
           AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKE+RYLVNATTFKGPCKDGMVIQIEGTIV
Sbjct: 203 AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIV 262

Query: 129 APDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRALTIDSSSN 188
           APDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRA TIDSSSN
Sbjct: 263 APDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRAFTIDSSSN 322

Query: 189 IRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKIST 248
           IRVKGLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KIST
Sbjct: 323 IRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKIST 382

Query: 249 GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI 308
           GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI
Sbjct: 383 GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI 442

Query: 309 KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT 368
           KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT
Sbjct: 443 KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT 502

Query: 369 TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSD 428
           TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSD
Sbjct: 503 TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSD 562

Query: 429 NDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           N+F A DQTQV ELE +QLPTEQHIVHTEL
Sbjct: 563 NNFAASDQTQVLELETVQLPTEQHIVHTEL 591

BLAST of HG10015226 vs. NCBI nr
Match: ADN33827.1 (polygalacturonase [Cucumis melo subsp. melo])

HSP 1 Score: 833.6 bits (2152), Expect = 8.4e-238
Identity = 415/442 (93.89%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 24  DIDMSKELESFDIEEEDDNAMDSYAVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRK 83
           DIDM +ELESFDIEEEDD+ + SY VP WRSERGSK+LMNVDSFDAVGDGVTDDTMAFRK
Sbjct: 169 DIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFDAVGDGVTDDTMAFRK 228

Query: 84  AWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQ 143
           AWETACSTSKSVLLVPKE+RYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPK+PRQ
Sbjct: 229 AWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKFPRQ 288

Query: 144 WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSR-------ALTIDSSSNIRVKGLTI 203
           WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSR       A TIDSSSNIRVKGLTI
Sbjct: 289 WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTAFTIDSSSNIRVKGLTI 348

Query: 204 QNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIV 263
           QNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KISTGDDCISIV
Sbjct: 349 QNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIV 408

Query: 264 NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG 323
           NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG
Sbjct: 409 NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG 468

Query: 324 YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK 383
           YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK
Sbjct: 469 YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK 528

Query: 384 FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFTALDQ 443
           FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDN+F ALDQ
Sbjct: 529 FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQ 588

Query: 444 TQVPELEAMQLPTEQHIVHTEL 459
           TQV ELE +QLPTEQHIVHTEL
Sbjct: 589 TQVLELETVQLPTEQHIVHTEL 610

BLAST of HG10015226 vs. NCBI nr
Match: KAG6573734.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 833.6 bits (2152), Expect = 8.4e-238
Identity = 416/469 (88.70%), Postives = 435/469 (92.75%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDKFFLL LL +LIIAYGVSGSI    DIDM KELE+FDIEE+DDN +D+YA+PVW+SER
Sbjct: 1   MDKFFLLPLLSLLIIAYGVSGSIDPESDIDMVKELETFDIEEQDDNVVDTYAIPVWKSER 60

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GS+ LMNVDSFDAVGDG+TDDTMAFRKAWETACS +KSVLLVPKE+RYLVNATTFKGPC 
Sbjct: 61  GSRSLMNVDSFDAVGDGITDDTMAFRKAWETACSAAKSVLLVPKEKRYLVNATTFKGPCN 120

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGM+IQIEGTI+APDEPE WDPK PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMIIQIEGTIIAPDEPEKWDPKLPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       ALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRITEVRVS+PGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSSPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNS GIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSAGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           +KVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRM+DVENPIIIDQFYCDSP  CE
Sbjct: 301 SKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMQDVENPIIIDQFYCDSPNACE 360

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSA+K+SQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 TQTSAIKVSQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYGVVHPSADCLTSSDNDF A DQT        QLPTEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNDFAAKDQT--------QLPTEQHIVHTEL 461

BLAST of HG10015226 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 4.2e-99
Identity = 187/389 (48.07%), Postives = 250/389 (64.27%), Query Frame = 0

Query: 53  RSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKERRYLVNATTF 112
           RSER    L++V +F A G+GVTDDT AF  AW+TACS+  K+ +LVP+    L+     
Sbjct: 48  RSER----LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDL 107

Query: 113 KGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAE 172
            GPCK  + +QI GTI+AP++P++W+    R+WL F  L+  T +G G ++G G++WW  
Sbjct: 108 SGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWER 167

Query: 173 SCKKNKSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGD 232
           SCK N S        ALT     N+RV+ L + +SQQMH  +     V I+ ++V AP  
Sbjct: 168 SCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 227

Query: 233 SPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDN 292
           SPNTDGIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGISIGSLGK  
Sbjct: 228 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSK 287

Query: 293 STGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDS 352
           S   V  + +DTA + +T NGVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS
Sbjct: 288 SWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDS 347

Query: 353 PTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTV 412
              C  QTSA+ I  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  
Sbjct: 348 RKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFT 407

Query: 413 ETYCHAAQGFGYGVVHPSADCLTSSDNDF 432
           E++C  A G   G V+P   CL S D  F
Sbjct: 408 ESFCWEAYGSSSGQVYP-PPCL-SDDTSF 430

BLAST of HG10015226 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.8e-90
Identity = 179/367 (48.77%), Postives = 235/367 (64.03%), Query Frame = 0

Query: 62  MNVDSFDAVGDGVTDDTMAFRKAWETAC-STSKSVLLVPKERRYLVNATTFKGPCKDGMV 121
           +NVD F A GDG  DDT AF KAW+ AC STS +VLLVPK + YLV   +F GPCK G+ 
Sbjct: 90  VNVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPK-KNYLVRPISFSGPCKSGLT 149

Query: 122 IQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKS-- 181
           +QI GTI A D+   +  K  R WL F  +     +G G I+G+G+ WW  SCK NK+  
Sbjct: 150 MQIYGTIEASDDRSDY-RKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALP 209

Query: 182 -----RALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHI 241
                 ALT   S ++ VK L I+N+QQ+H        V+ + + V+AP +SPNTDGIH+
Sbjct: 210 CKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHV 269

Query: 242 TQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVV 301
           T + N+ + +C I TGDDCISIVN S  +++  I+CGPGHGISIGSLG  NS   V+ VV
Sbjct: 270 TGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVV 329

Query: 302 LDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTS 361
           ++ A L  TTNGVRIKTWQGGSG   +++F+NV M +VENPIIIDQ YCD    C+ Q+S
Sbjct: 330 VNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSS 389

Query: 362 AVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDG-TVETYCHAAQGF 420
           AV++  ++Y+NI GT  S  A+ F CS   PC  I+L +V+LE   G   +  C+  +  
Sbjct: 390 AVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELS 449

BLAST of HG10015226 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-89
Identity = 166/376 (44.15%), Postives = 229/376 (60.90%), Query Frame = 0

Query: 60  VLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGM 119
           ++ NV+ + AVGDG  D T AF K W  AC+   +V LVP  ++++VN   F GPC+   
Sbjct: 58  IVFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGPCQPHF 117

Query: 120 VIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCK----- 179
             +++GTI A  +P  W  K  + W+ F +L      G GVIDG G +WW++ CK     
Sbjct: 118 SFKVDGTIAAYPDPAKW--KNSKIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGR 177

Query: 180 -----KNKSRALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 239
                K +  A+ ID S ++ VK LT+ NS + H +    + V+I  +++ AP DSPNTD
Sbjct: 178 TVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPNTD 237

Query: 240 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 299
           GI I  S    ++ C I TGDDC+++   SS I +K ++CGPGHG+SIGSLGK NS   V
Sbjct: 238 GIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRSEV 297

Query: 300 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 359
           + V LD A   +T NG+RIKTWQGGSG    + +ENV M + ENPI+I+QFYC S   C+
Sbjct: 298 SFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAAACK 357

Query: 360 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA- 419
            Q SAVKI  + ++NI GT+ +  A++  CSDSVPCSNI LSNV L+ T G V T  +  
Sbjct: 358 NQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKVATCVNKN 417

Query: 420 AQGFGYGVVHPSADCL 425
           A G+    ++PS   L
Sbjct: 418 ANGYYTNPLNPSCKSL 431

BLAST of HG10015226 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.8e-89
Identity = 168/378 (44.44%), Postives = 233/378 (61.64%), Query Frame = 0

Query: 54  SERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKG 113
           S   +  + NV+ + AVGDG  D T AF   W  AC  + +VLLVP  +++ VN   F+G
Sbjct: 51  SRHDAATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRG 110

Query: 114 PCKDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESC 173
           PC+  +  +++GTIVA  +P  W  K  + WL+F +L      G GVIDG G++WWA  C
Sbjct: 111 PCQPHLSFKVDGTIVAQPDPARW--KNSKIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQC 170

Query: 174 K----------KNKSRALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPG 233
           K          +N+  A+ ID S ++ VK LT+ NS + H +    E V+I  +++ AP 
Sbjct: 171 KVVNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPR 230

Query: 234 DSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKD 293
           DSPNTDGI I  S    ++ C I TGDDCI+I   SS I +K + CGPGHGISIGSLG+D
Sbjct: 231 DSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRD 290

Query: 294 NSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCD 353
           NS   V+ V ++ A   +T NG+RIKTWQGGSG    + +ENV M + ENPI+I+QFYC 
Sbjct: 291 NSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCT 350

Query: 354 SPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVE 413
           S + C+ Q SAV+I  + Y+NI GT+ +  A++  CSDSVPC+ I LSNV+L+ T G   
Sbjct: 351 SASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPA 410

Query: 414 TYCHA-AQGFGYGVVHPS 421
           +     A+GF  G + P+
Sbjct: 411 SCVDKNARGFYSGRLIPT 426

BLAST of HG10015226 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.8e-89
Identity = 172/362 (47.51%), Postives = 227/362 (62.71%), Query Frame = 0

Query: 50  PVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNAT 109
           PV   E      ++VD F A GDG TDDT AF KAW+ ACS S SVL+VP+ + YL+   
Sbjct: 73  PVGGPETSPDTDISVDDFGARGDG-TDDTKAFEKAWKDACS-SGSVLIVPENKNYLLKQI 132

Query: 110 TFKGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWW 169
           TF GPCK  + ++I GTI A  +   W     ++W+EF  ++  T +G G  +G+GE WW
Sbjct: 133 TFSGPCKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW 192

Query: 170 AESCKKNKS-------RALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAP 229
             SCK+ KS        ALT  S  N+ V  L+I++SQ+MH    + + V  + + V+AP
Sbjct: 193 DSSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAP 252

Query: 230 GDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGK 289
             SPNTDGIHIT +  + V N  I TGDDCISI + S  +    I+CGPGHGISIGSLG 
Sbjct: 253 EHSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGD 312

Query: 290 DNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYC 349
            NS   V+ V++D   L +TTNG+RIKTWQGGSG  ++++F+N+ M +V NPIIIDQ+YC
Sbjct: 313 RNSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYC 372

Query: 350 DSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTV 405
           DS   C  Q SAVK+S + Y NI GT+ S+ A+KF CS S PC   I+ N+NL    G  
Sbjct: 373 DSKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKE 432

BLAST of HG10015226 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.2e-247
Identity = 434/469 (92.54%), Postives = 445/469 (94.88%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDK FLLSLLG+LIIAYGVSG+     DIDM +ELESFDIEEEDD+ + SY VP WRSER
Sbjct: 38  MDKLFLLSLLGLLIIAYGVSGTFVSDSDIDMIEELESFDIEEEDDDVVYSYTVPAWRSER 97

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GSK+LMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKE+RYLVNATTFKGPCK
Sbjct: 98  GSKILMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCK 157

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMVIQIEGTIVAPDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 158 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 217

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       A TIDSSSNIRVKGLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTD
Sbjct: 218 KSRPCKGAPTAFTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTD 277

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVVQN KISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV
Sbjct: 278 GIHITQSTNVVVQNSKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 337

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE
Sbjct: 338 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 397

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 398 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 457

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYG+VHPSADCLTSSDN+F ALDQTQV ELE +QLPTEQHIVHTEL
Sbjct: 458 QGFGYGIVHPSADCLTSSDNNFAALDQTQVLELETVQLPTEQHIVHTEL 506

BLAST of HG10015226 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.0e-244
Identity = 429/469 (91.47%), Postives = 443/469 (94.46%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDK FLLSLLG+ IIAYGVSG+     DIDM KELESFDIEEEDD+ + SY VPVWRSER
Sbjct: 1   MDKLFLLSLLGLFIIAYGVSGAFVSDSDIDMIKELESFDIEEEDDDDVYSYTVPVWRSER 60

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GSK+LMNVDSFDA+GDGVTDDTMAFRKAWETACSTSKSVLLVPKE+ YLVNATTFKGPCK
Sbjct: 61  GSKILMNVDSFDAIGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKTYLVNATTFKGPCK 120

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
           DGMVIQIEGTIVAPDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN
Sbjct: 121 DGMVIQIEGTIVAPDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       ALTIDSSSNIRVKGLTIQNSQQMHF IARSETVRIT VRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFTIARSETVRITGVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV
Sbjct: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           TKVVLDTA+LRETTNGVRIKTWQGGSGYVR+VRFENVRMEDVENPIIIDQFYCDSPTTCE
Sbjct: 301 TKVVLDTAYLRETTNGVRIKTWQGGSGYVRAVRFENVRMEDVENPIIIDQFYCDSPTTCE 360

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
           +QTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 SQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYGVVHPSADCLTSSDN+F + DQTQV ELE +QL TEQHIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLTSSDNNFASSDQTQVLELETVQLSTEQHIVHTEL 469

BLAST of HG10015226 vs. ExPASy TrEMBL
Match: A0A5D3CXE4 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001110 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.3e-239
Identity = 421/450 (93.56%), Postives = 430/450 (95.56%), Query Frame = 0

Query: 9   LLGMLIIAYGVSGSIDIDMSKELESFDIEEEDDNAMDSYAVPVWRSERGSKVLMNVDSFD 68
           LL   II   VS S DIDM +ELESFDIEEEDD+ + SY VP WRSERGSK+LMNVDSFD
Sbjct: 143 LLVNSIIGTFVSDS-DIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFD 202

Query: 69  AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGMVIQIEGTIV 128
           AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKE+RYLVNATTFKGPCKDGMVIQIEGTIV
Sbjct: 203 AVGDGVTDDTMAFRKAWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIV 262

Query: 129 APDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRALTIDSSSN 188
           APDEPELWDPK+PRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRA TIDSSSN
Sbjct: 263 APDEPELWDPKFPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRAFTIDSSSN 322

Query: 189 IRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKIST 248
           IRVKGLTIQNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KIST
Sbjct: 323 IRVKGLTIQNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKIST 382

Query: 249 GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI 308
           GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI
Sbjct: 383 GDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRI 442

Query: 309 KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT 368
           KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT
Sbjct: 443 KTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGT 502

Query: 369 TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSD 428
           TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSD
Sbjct: 503 TTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSD 562

Query: 429 NDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           N+F A DQTQV ELE +QLPTEQHIVHTEL
Sbjct: 563 NNFAASDQTQVLELETVQLPTEQHIVHTEL 591

BLAST of HG10015226 vs. ExPASy TrEMBL
Match: E5GBI5 (Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.1e-238
Identity = 415/442 (93.89%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 24  DIDMSKELESFDIEEEDDNAMDSYAVPVWRSERGSKVLMNVDSFDAVGDGVTDDTMAFRK 83
           DIDM +ELESFDIEEEDD+ + SY VP WRSERGSK+LMNVDSFDAVGDGVTDDTMAFRK
Sbjct: 169 DIDMIEELESFDIEEEDDDVVYSYTVPAWRSERGSKILMNVDSFDAVGDGVTDDTMAFRK 228

Query: 84  AWETACSTSKSVLLVPKERRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQ 143
           AWETACSTSKSVLLVPKE+RYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPK+PRQ
Sbjct: 229 AWETACSTSKSVLLVPKEKRYLVNATTFKGPCKDGMVIQIEGTIVAPDEPELWDPKFPRQ 288

Query: 144 WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSR-------ALTIDSSSNIRVKGLTI 203
           WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSR       A TIDSSSNIRVKGLTI
Sbjct: 289 WLEFTKLNAATFQGDGVIDGSGEKWWAESCKKNKSRPCKGAPTAFTIDSSSNIRVKGLTI 348

Query: 204 QNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIV 263
           QNSQQMHF IARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQN KISTGDDCISIV
Sbjct: 349 QNSQQMHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIV 408

Query: 264 NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG 323
           NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG
Sbjct: 409 NASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSG 468

Query: 324 YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK 383
           YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK
Sbjct: 469 YVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMK 528

Query: 384 FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNDFTALDQ 443
           FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYG+VHPSADCLTSSDN+F ALDQ
Sbjct: 529 FACSDSVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQ 588

Query: 444 TQVPELEAMQLPTEQHIVHTEL 459
           TQV ELE +QLPTEQHIVHTEL
Sbjct: 589 TQVLELETVQLPTEQHIVHTEL 610

BLAST of HG10015226 vs. ExPASy TrEMBL
Match: A0A6J1GXR9 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111458447 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.3e-238
Identity = 421/469 (89.77%), Postives = 438/469 (93.39%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIAYGVSGSI----DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDKFFLLSLLGMLII YGVSGSI    DIDM KELESFDIEEEDD+A+D+YAVP WRSE 
Sbjct: 1   MDKFFLLSLLGMLIIVYGVSGSIVPDGDIDMVKELESFDIEEEDDSAVDTYAVPAWRSEH 60

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
           GSK+L+NVDSFDAVGDGVTDDTMAFRKAWETACS+S SVLLVPKE RYLVNAT FKGPCK
Sbjct: 61  GSKILINVDSFDAVGDGVTDDTMAFRKAWETACSSSNSVLLVPKEGRYLVNATKFKGPCK 120

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
            GMVIQIEGTIVAPDEPE WDPK PRQWLEFTKL AATFQGDG+IDGSGEKWWAESCKKN
Sbjct: 121 HGMVIQIEGTIVAPDEPENWDPKLPRQWLEFTKLTAATFQGDGIIDGSGEKWWAESCKKN 180

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KSR       ALTIDSSSNIRVKGLTIQNSQQMHFIIARS+TVR++EVRVSAPGDSPNTD
Sbjct: 181 KSRPCKGAPTALTIDSSSNIRVKGLTIQNSQQMHFIIARSKTVRVSEVRVSAPGDSPNTD 240

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHITQSTNVVV+NCKISTGDDCISIVNASSGIKMK ISCGPGHGISIGSLGKDNSTGIV
Sbjct: 241 GIHITQSTNVVVENCKISTGDDCISIVNASSGIKMKRISCGPGHGISIGSLGKDNSTGIV 300

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           TKVVLDTAFLRETTNGVRIKTWQGGSGYVR+VRFENVRME+VENPIIIDQFYCD  TTCE
Sbjct: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRAVRFENVRMENVENPIIIDQFYCDH-TTCE 360

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
            Q SAVKISQIMYRNISGTTTSKNAMKFACSDS PCSNIILSNVNLEKTDGTVETYCHAA
Sbjct: 361 PQASAVKISQIMYRNISGTTTSKNAMKFACSDSFPCSNIILSNVNLEKTDGTVETYCHAA 420

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           QGFGYGVVHPSADCL+SSDND  A D+TQVPELEA+QLPTE+HIVHTEL
Sbjct: 421 QGFGYGVVHPSADCLSSSDNDIAASDETQVPELEAVQLPTERHIVHTEL 468

BLAST of HG10015226 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 616.3 bits (1588), Expect = 2.0e-176
Identity = 307/452 (67.92%), Postives = 364/452 (80.53%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLII-AYGVSGSI---DIDMSKELESFDIEEEDDNAMDSYAVPVWRSER 60
           MDK F+LSLLG+L++ AYG +G +   D+D+  ELE+FD+  +DD+       P + S  
Sbjct: 2   MDKLFILSLLGLLLVTAYGAAGKMVYTDLDILDELENFDVLVDDDDDTKLLDWPSFTSRH 61

Query: 61  GSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCK 120
             K L+NVD+F A GDGV+DDT AF  AW  ACSTSKSV LVP+ RRYLVNAT F GPC+
Sbjct: 62  SGKNLVNVDTFGAAGDGVSDDTQAFVSAWSKACSTSKSVFLVPEGRRYLVNATKFNGPCE 121

Query: 121 DGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 180
             ++IQI+GTIVAPDEP  WD K+ R WLEF+KL    FQG GVIDGSG KWWA SCKKN
Sbjct: 122 QKLIIQIDGTIVAPDEPSNWDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKKN 181

Query: 181 KSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTD 240
           KS        ALTI+SSS ++V GLTIQNSQQM+FIIARS++VR+++V VS+PGDSPNTD
Sbjct: 182 KSNPCKSAPTALTIESSSGVKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNTD 241

Query: 241 GIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIV 300
           GIHIT STNV++Q+CKI TGDDC+SIVNASS IKMK I CGPGHGISIGSLGKDN+TGIV
Sbjct: 242 GIHITGSTNVILQDCKIGTGDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGIV 301

Query: 301 TKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCE 360
           T+VVLDTA LRETTNG+RIKT+QGGSGYV+ +RF NV M+DV NPI+IDQFYCDSPTTC+
Sbjct: 302 TQVVLDTALLRETTNGLRIKTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTCQ 361

Query: 361 TQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHAA 420
            QTSAVKISQIMYRNI+GTT S  A+KFACSD+VPCS+I+L+NVNLE  DG VE YC++A
Sbjct: 362 NQTSAVKISQIMYRNITGTTKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVEAYCNSA 421

Query: 421 QGFGYGVVHPSADCLTSSDNDFTALDQTQVPE 442
           +GFGYGV+HPSADCL S D+    LDQT   E
Sbjct: 422 EGFGYGVIHPSADCLYSHDD--KGLDQTHKSE 451

BLAST of HG10015226 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 575.1 bits (1481), Expect = 5.0e-164
Identity = 291/470 (61.91%), Postives = 362/470 (77.02%), Query Frame = 0

Query: 1   MDKFFLLSLLGMLIIA-YGVSGSI---DIDMSKELESFDIEEED-DNAMDSYAVPVWRSE 60
           M K F++SL  +L++A YG +G +   ++D+  +LE+ D+ E+D ++ +  +    + S+
Sbjct: 1   MIKPFIISLFALLMVASYGATGKMLFKELDILGKLENLDVPEDDIEDDVTFFDFSSFTSQ 60

Query: 61  RGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPC 120
              K L+NVDSF+A GDGV+DDT AF +AW  ACS   SVLLVP+ R YLVNAT F GPC
Sbjct: 61  YSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFDGPC 120

Query: 121 KDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKK 180
           ++ ++IQI+GTI+APDEP  WDPK+PR WL+F+KL    FQG+GVIDGSG KWWA SCKK
Sbjct: 121 QEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCKK 180

Query: 181 NKSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNT 240
           NKS        ALTI SSSN+ V+GLTI+NSQQMH II RS TVRI+ V V++PGDSPNT
Sbjct: 181 NKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPNT 240

Query: 241 DGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGI 300
           DGIHIT ST+VVVQ+ KISTGDDC+SIVN S+ IKMK I CGPGHGISIGSLG+ +S G 
Sbjct: 241 DGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHSKGT 300

Query: 301 VTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTC 360
           VT VVL+TAFL+ TTNG+RIKTWQGG+GYV+ VRFENV M+DV NPIIIDQFYCDSP+TC
Sbjct: 301 VTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPSTC 360

Query: 361 ETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKTDGTVETYCHA 420
           + QTSAV IS+IMYRNI+GTT S  A+ F CSD+VPCS+I+L+N+NLE  DG VE YC++
Sbjct: 361 QNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVEAYCNS 420

Query: 421 AQGFGYGVVHPSADCLTSSDNDFTALDQTQVPELEAMQLPTEQHIVHTEL 459
           A+GFGYGVVHPSADCL S D+   +L+QT     E + +  E    H EL
Sbjct: 421 AEGFGYGVVHPSADCLYSHDD--KSLNQTDQYLAETIMVEKETENAHDEL 468

BLAST of HG10015226 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 363.2 bits (931), Expect = 3.0e-100
Identity = 187/389 (48.07%), Postives = 250/389 (64.27%), Query Frame = 0

Query: 53  RSERGSKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKERRYLVNATTF 112
           RSER    L++V +F A G+GVTDDT AF  AW+TACS+  K+ +LVP+    L+     
Sbjct: 48  RSER----LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDL 107

Query: 113 KGPCKDGMVIQIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQGDGVIDGSGEKWWAE 172
            GPCK  + +QI GTI+AP++P++W+    R+WL F  L+  T +G G ++G G++WW  
Sbjct: 108 SGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWER 167

Query: 173 SCKKNKSR-------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGD 232
           SCK N S        ALT     N+RV+ L + +SQQMH  +     V I+ ++V AP  
Sbjct: 168 SCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 227

Query: 233 SPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDN 292
           SPNTDGIHI+ S  +V+ N  +STGDDCISIV  S+ I +  I CGPGHGISIGSLGK  
Sbjct: 228 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSK 287

Query: 293 STGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDS 352
           S   V  + +DTA + +T NGVRIKTWQGGSG V  + F N++M +V NPIIIDQ+YCDS
Sbjct: 288 SWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDS 347

Query: 353 PTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLEKT--DGTV 412
              C  QTSA+ I  I + ++ GT+ SK A+K +CSDS PC NI+L +++LE +  DG  
Sbjct: 348 RKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFT 407

Query: 413 ETYCHAAQGFGYGVVHPSADCLTSSDNDF 432
           E++C  A G   G V+P   CL S D  F
Sbjct: 408 ESFCWEAYGSSSGQVYP-PPCL-SDDTSF 430

BLAST of HG10015226 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.2e-87
Identity = 164/384 (42.71%), Postives = 234/384 (60.94%), Query Frame = 0

Query: 58  SKVLMNVDSFDAVGDGVTDDTMAFRKAWETACSTSKSVLLVPKERRYLVNATTFKGPCKD 117
           S  + +V SF AVGDG  DDT AF+ AW+ AC+    V+L P+   + + +T F GPCK 
Sbjct: 76  SGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKP 135

Query: 118 GMVIQIEGTIVAPDEPELWDPK-YPRQWLEFTKLNAATFQGDGVIDGSGEKWWAESCKKN 177
           G+V Q++G ++ PD PE W  K    QWL F +L+  TF G G ++G+G+KWW   CK +
Sbjct: 136 GLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPH 195

Query: 178 KS-------------RALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPG 237
           +                +    S+NI VKGL IQNS Q H      + V I E+++S+P 
Sbjct: 196 RGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPK 255

Query: 238 DSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKD 297
            SPNTDGIH+  + +V + N  +S GDDCISI    S + ++G++CGP HGISIGSLG  
Sbjct: 256 LSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVH 315

Query: 298 NSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCD 357
           NS   V+ + +    +R++ NG+R+KTWQGG+G V ++ FEN++ME+V N II+DQ+YC 
Sbjct: 316 NSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQ 375

Query: 358 SPTTCETQTSAVKISQIMYRNISGTTTSKN-AMKFACSDSVPCSNIILSNVNLEKTDGTV 417
           S   C  +TSAVK+  + YRNI GT   ++  + FACSD+V C+NI +S V L   +G +
Sbjct: 376 S-KDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGEL 435

Query: 418 --ETYCHAAQGFGYGVVHPSADCL 425
             + +C  A G    +  P  DCL
Sbjct: 436 VDDPFCWNAYGKQETLTIPPIDCL 458

BLAST of HG10015226 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-86
Identity = 177/425 (41.65%), Postives = 257/425 (60.47%), Query Frame = 0

Query: 7   LSLLGMLIIAYGVSGSIDIDMSKELESFD----IEEEDDNAMDSYAVPVWRSERGSKVLM 66
           L +L ML +   +S ++D     E  SF+    ++  +D+ +   +      E  +   +
Sbjct: 12  LCVLLMLSLCKALSSNVDDGYGHEDGSFESDSLLKLNNDDVLSLISSDETTLEAST---V 71

Query: 67  NVDSFDAVGDGVTDDTMAFRKAWETACSTS-KSVLLVPKERRYLVNATTFKGPCKDGMVI 126
           +V +F A GDG TDDT AF+KAW+ ACST+  +  LVPK + YL+ +T F+GPCK     
Sbjct: 72  SVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKSLRNF 131

Query: 127 QIEGTIVAPDEPELWDPKYPRQWLEFTKLNAATFQG--DGVIDGSGEKWWAESCKKNKSR 186
           QI GT+ A  +    D K    WL    +N  +  G   G+I+G+G+ WW  SCK +KS+
Sbjct: 132 QILGTLSASTKRS--DYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKSK 191

Query: 187 -------ALTIDSSSNIRVKGLTIQNSQQMHFIIARSETVRITEVRVSAPGDSPNTDGIH 246
                  ALT+ +  N+ VK L ++N+QQ+   I +   V ++ V ++APGDSPNTDGIH
Sbjct: 192 PCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIH 251

Query: 247 ITQSTNVVVQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKV 306
           IT + N+ V N  I TGDDCISI + +  +++  ++CGPGHGISIGSLG DNS   V+ +
Sbjct: 252 ITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGI 311

Query: 307 VLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTTCETQT 366
            +D A   E+ NGVRIKT+QGGSG  ++++F+N+RME+V+NPIIIDQ YCD    CE Q 
Sbjct: 312 NVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDK-DKCEDQE 371

Query: 367 SAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIILSNVNLE-----------KTDGT 407
           SAV++  ++Y+NISGT+ +  A+   CS+  PC  I+L NV ++           K  GT
Sbjct: 372 SAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIKGGTASCKNANVKNQGT 430

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446196.11.7e-24692.54PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo][more]
XP_011655619.11.0e-24391.47probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus][more]
TYK15614.12.6e-23993.56polygalacturonase [Cucumis melo var. makuwa][more]
ADN33827.18.4e-23893.89polygalacturonase [Cucumis melo subsp. melo][more]
KAG6573734.18.4e-23888.70putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q94AJ54.2e-9948.07Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353362.8e-9048.77Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q9FY191.0e-8944.15Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q7M1E71.8e-8944.44Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q020966.8e-8947.51Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BF588.2e-24792.54probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=... [more]
A0A0A0KQ655.0e-24491.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1[more]
A0A5D3CXE41.3e-23993.56Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
E5GBI54.1e-23893.89Polygalacturonase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A6J1GXR95.3e-23889.77probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114584... [more]
Match NameE-valueIdentityDescription
AT1G23460.12.0e-17667.92Pectin lyase-like superfamily protein [more]
AT1G70500.15.0e-16461.91Pectin lyase-like superfamily protein [more]
AT1G80170.13.0e-10048.07Pectin lyase-like superfamily protein [more]
AT1G48100.12.2e-8742.71Pectin lyase-like superfamily protein [more]
AT3G57510.11.5e-8641.65Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 259..279
e-value: 8900.0
score: 0.5
coord: 318..339
e-value: 4300.0
score: 2.7
coord: 385..406
e-value: 7500.0
score: 1.0
coord: 236..257
e-value: 18.0
score: 14.2
coord: 209..235
e-value: 75.0
score: 12.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 91..406
e-value: 1.4E-90
score: 303.7
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 266..279
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 50..457
e-value: 2.8E-120
score: 404.0
NoneNo IPR availablePANTHERPTHR31375:SF205BNAA08G20470D PROTEINcoord: 2..436
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 2..436
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 59..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10015226.1HG10015226.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0004650 polygalacturonase activity