HG10015164 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10015164
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCytochrome P450
LocationChr02: 24430779 .. 24433161 (-)
RNA-Seq ExpressionHG10015164
SyntenyHG10015164
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCTTCTTCTCCTCATTCTCCTATTCTCTCTACTTGTATTGATCCTAAAATGGAAGAAAAATGTAAAGAAAAAAAAGGGAAATTTTCCCCCAAGTCCACCAAAGCTTCCGATCATAGGAAACTTACACCAACTTGGGAAGCTCCCACACAAATCTCTATGGCGTCTCTCTCAGCTTTATGGCCCCATCATTAGCCTCAACCTCGGCGGCATTCAAACCACCGTCATCTCCTCCGCCTATGCCGCCCGTCGTCTCTTAAAAACACACGATCTTCAATCCTGCAGTCGACCACAAATCGAAAGTGCCAGAAAACTCACTCACAATTTCCTCGACATTGCGTTTTCCCCTTACGGTGATTACTGGCGAGAGATGAGGGAAATTTGTGTTGTTGAGCTTTTTAGTCTCAAGAGACTGAAATCTTACGAACCCATTAGAGATCAAGAAATGAGTTGGCTTATTGAATCAATTTCTGAAGTGGCTTCTTGTGGGGGCCCTGTTGATCTTACTCAGAAAACAGTGGCTCTGACTGCTGCTATTATTTTTAGAATTGCTTTTGGGAAGAAGGTGTTTGAAAAAGGGGATGGATTTCATGAGCTTGTGAATGAAGCTGAGGCTTTGTTGGGAACTTATAGTGCTTCTGAATTGTTTCCTTATTATGTTCCTAATTTTGTGGGGAAGCTAATTGATTGTGTTACTGGATATCAAACAAGGCTTCATAAGGTTTTTAATGAATTGAATGGTTTGTTTCAAGAAGTGATTGATGAACATCTTTGTCCTGAGAGGGATAAACCAGAGGAAGATGATATTATTGATGTGCTTTTGGGAATTAGCAAAAATCAAGCTCTATCAGCTGCTTCTAATGTTTCCATTACCCATGAGAATATCAAAGGCGTTCTTTTGGTAACCAAACTTTTTTCATTATCTATCTTTAATGTCCCTTGTTATTGGTTGAAATACAAGTTTAGTTTCTTAAAATGCTGAACTGTTTTTTAAATTTTAGAGTAGTTAATTTAGCTGCTAGTGGAGCTACGAACTTTCTATCACTTTGACAAGTTAATTTTTTTTTTAATACTTAGATGCATTCTAGTTTGCACCCAATCTTTTGTTCTGTTGTTGACGTAGGCTTAAAAAAATGGATGTTGTCTAATTATTCAATAAAAATTTGTTAGGTGGCAATATTTTTTTTAAAAAAAATTAAATTATTATAAGAGTTGTTTTCAAATATAGAAAAATGAGTTAAACTATTTACCAATATATAAACATTTCACTTTCTATCAACGATAGACCATGATAGAATTCTATCGCTTGAGCGATAAACTTCATTTACAAATATAAAAAAAAATTAACTATTTATTAGTGATAGACTGCGATAGAATTCTATCGCTTGTCTATCGCTCAAGCGATAGAATTCAATCACGGTCTATCGTGGATAAACATTAAAAAAAATTTATATTAGTAAATAGTTTAATATTTTTTTTGAAAAATTTTCACAAATAAAAAAAATGTCAAACTATTAACATAAAATAGCAAAAAAAATACTGATAGACATTGATAGACTTCTATCAGCATCTATTAGTGATAGACTTCGATAGATCCTGATAGACTTCTATCAGCGTCTATAAAAACTATTTAAAAATTTTGCTATTTTGTGTAAATAGTTTTCCTTGTTTTTCTATTTTTAAAACTTTTTTTTTTTTTTTTTGTATATAGTAATTTTTTTTCTTATTATTATTTTACTATGTCGAAAAGAAAAAAAAAATTTGTTCCACCTAATCGTATACCTTTGTTTTTACTAGTATGTGTGTTCTTCTTGCCTTGTGTTTCTCTATTATCTCACAGCCAATTTTCTAACAAACCGTATTGATAAATGTGAATGAACAGAAAGAAGTTACATCGGAAACAGAGGAAAAGTAACAGAGAAAGACACAGAGGAGCTTCCATATCTGAAAATGATAGTGAAAGAAACTCTGAGATTGCATCCTCCAGCACCCCTTCTTCTTCCAAGAGAAATCATATCCCATTTCGAAATGGAAGGCTTCGATTTTTACCCAAAAACAGTGGTCCAAGTCAATGCTTGGGCAATTGGACGAGACCCCAAATGCTGGAAAGACCCAGAAGAGTTGCAGAGAGCTGTGTTGATTACAGAGGACAGCATTTTGAGTTGCTGCCGTTTGGGGCTGGCCGGAGGATTTGTCCGGCGTTGAATATGGGAATCAAAATTGTGGAGTTTGCTTTGGCGAATCTTTTGTACCATTTTGATTGGAAGTTGCCGGAAGGGATGAAGGAGGAGGATTTGGACATGGAAGAAACCTCTGGGTTTAGCCTCACCATTTACAAGAAGGTGCCTCTCAAACTTGTTCCTGTTTCATACCATCCATGA

mRNA sequence

ATGTCTCTTCTTCTCCTCATTCTCCTATTCTCTCTACTTGTATTGATCCTAAAATGGAAGAAAAATGTAAAGAAAAAAAAGGGAAATTTTCCCCCAAGTCCACCAAAGCTTCCGATCATAGGAAACTTACACCAACTTGGGAAGCTCCCACACAAATCTCTATGGCGTCTCTCTCAGCTTTATGGCCCCATCATTAGCCTCAACCTCGGCGGCATTCAAACCACCGTCATCTCCTCCGCCTATGCCGCCCGTCGTCTCTTAAAAACACACGATCTTCAATCCTGCAGTCGACCACAAATCGAAAGTGCCAGAAAACTCACTCACAATTTCCTCGACATTGCGTTTTCCCCTTACGGTGATTACTGGCGAGAGATGAGGGAAATTTGTGTTGTTGAGCTTTTTAGTCTCAAGAGACTGAAATCTTACGAACCCATTAGAGATCAAGAAATGAGTTGGCTTATTGAATCAATTTCTGAAGTGGCTTCTTGTGGGGGCCCTGTTGATCTTACTCAGAAAACAGTGGCTCTGACTGCTGCTATTATTTTTAGAATTGCTTTTGGGAAGAAGGTGTTTGAAAAAGGGGATGGATTTCATGAGCTTGTGAATGAAGCTGAGGCTTTGTTGGGAACTTATAGTGCTTCTGAATTGTTTCCTTATTATGTTCCTAATTTTGTGGGGAAGCTAATTGATTGTGTTACTGGATATCAAACAAGGCTTCATAAGGTTTTTAATGAATTGAATGGTTTGTTTCAAGAAGTGATTGATGAACATCTTTGTCCTGAGAGGGATAAACCAGAGGAAGATGATATTATTGATGTGCTTTTGGGAATTAGCAAAAATCAAGCTCTATCAGCTGCTTCTAATGTTTCCATTACCCATGAGAATATCAAAGGCGTTCTTTTGAGAAATCATATCCCATTTCGAAATGGAAGGCTTCGATTTTTACCCAAAAACAGTGGTCCAAGTCAATGCTTGGGCAATTGGACGAGACCCCAAATGCTGGAAAGACCCAGAAGAGTTGCAGAGAGCTGTGTTGATTACAGAGGACAGCATTTTGAGTTGCTGCCGTTTGGGGCTGGCCGGAGGATTTGTCCGGCGTTGAATATGGGAATCAAAATTGTGGAGTTTGCTTTGGCGAATCTTTTGTACCATTTTGATTGGAAGTTGCCGGAAGGGATGAAGGAGGAGGATTTGGACATGGAAGAAACCTCTGGGTTTAGCCTCACCATTTACAAGAAGGTGCCTCTCAAACTTGTTCCTGTTTCATACCATCCATGA

Coding sequence (CDS)

ATGTCTCTTCTTCTCCTCATTCTCCTATTCTCTCTACTTGTATTGATCCTAAAATGGAAGAAAAATGTAAAGAAAAAAAAGGGAAATTTTCCCCCAAGTCCACCAAAGCTTCCGATCATAGGAAACTTACACCAACTTGGGAAGCTCCCACACAAATCTCTATGGCGTCTCTCTCAGCTTTATGGCCCCATCATTAGCCTCAACCTCGGCGGCATTCAAACCACCGTCATCTCCTCCGCCTATGCCGCCCGTCGTCTCTTAAAAACACACGATCTTCAATCCTGCAGTCGACCACAAATCGAAAGTGCCAGAAAACTCACTCACAATTTCCTCGACATTGCGTTTTCCCCTTACGGTGATTACTGGCGAGAGATGAGGGAAATTTGTGTTGTTGAGCTTTTTAGTCTCAAGAGACTGAAATCTTACGAACCCATTAGAGATCAAGAAATGAGTTGGCTTATTGAATCAATTTCTGAAGTGGCTTCTTGTGGGGGCCCTGTTGATCTTACTCAGAAAACAGTGGCTCTGACTGCTGCTATTATTTTTAGAATTGCTTTTGGGAAGAAGGTGTTTGAAAAAGGGGATGGATTTCATGAGCTTGTGAATGAAGCTGAGGCTTTGTTGGGAACTTATAGTGCTTCTGAATTGTTTCCTTATTATGTTCCTAATTTTGTGGGGAAGCTAATTGATTGTGTTACTGGATATCAAACAAGGCTTCATAAGGTTTTTAATGAATTGAATGGTTTGTTTCAAGAAGTGATTGATGAACATCTTTGTCCTGAGAGGGATAAACCAGAGGAAGATGATATTATTGATGTGCTTTTGGGAATTAGCAAAAATCAAGCTCTATCAGCTGCTTCTAATGTTTCCATTACCCATGAGAATATCAAAGGCGTTCTTTTGAGAAATCATATCCCATTTCGAAATGGAAGGCTTCGATTTTTACCCAAAAACAGTGGTCCAAGTCAATGCTTGGGCAATTGGACGAGACCCCAAATGCTGGAAAGACCCAGAAGAGTTGCAGAGAGCTGTGTTGATTACAGAGGACAGCATTTTGAGTTGCTGCCGTTTGGGGCTGGCCGGAGGATTTGTCCGGCGTTGAATATGGGAATCAAAATTGTGGAGTTTGCTTTGGCGAATCTTTTGTACCATTTTGATTGGAAGTTGCCGGAAGGGATGAAGGAGGAGGATTTGGACATGGAAGAAACCTCTGGGTTTAGCCTCACCATTTACAAGAAGGTGCCTCTCAAACTTGTTCCTGTTTCATACCATCCATGA

Protein sequence

MSLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKGVLLRNHIPFRNGRLRFLPKNSGPSQCLGNWTRPQMLERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEEDLDMEETSGFSLTIYKKVPLKLVPVSYHP
Homology
BLAST of HG10015164 vs. NCBI nr
Match: XP_038891462.1 (cytochrome P450 71B2-like [Benincasa hispida])

HSP 1 Score: 591.3 bits (1523), Expect = 6.8e-165
Identity = 334/509 (65.62%), Postives = 363/509 (71.32%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVL-ILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           MSLLLLILLFSLLVL ILKWKKN  +KKG+FPP+PPKLPIIGNLHQLGKLPHKSLWRLSQ
Sbjct: 29  MSLLLLILLFSLLVLIILKWKKNRNRKKGHFPPTPPKLPIIGNLHQLGKLPHKSLWRLSQ 88

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
           LYGPIISLNLG I+TTVISS  AA  LLKTHDLQ+CSRPQ ESARK THNFLDIAFSPYG
Sbjct: 89  LYGPIISLNLGSIRTTVISSPTAAHGLLKTHDLQTCSRPQTESARKFTHNFLDIAFSPYG 148

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           +YWREMR+ICV+ELFSLKRLKSYEPI DQE+S LIES SE+ASCG  VD++ K++ALTAA
Sbjct: 149 EYWREMRKICVLELFSLKRLKSYEPIIDQEVSSLIESTSELASCGDVVDVSDKSMALTAA 208

Query: 181 IIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRL 240
           IIFRIAFGKKV E GDGFHE+V+EAEALLG+YS  ELF    PNFVGK+ID  +G Q R+
Sbjct: 209 IIFRIAFGKKVSE-GDGFHEVVSEAEALLGSYSGFELF----PNFVGKVIDWFSGRQRRV 268

Query: 241 HKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLG-ISKNQALSAASNVSITHENIKG 300
            KVFNEL+ LFQEVIDEHL   RDKPE+DDI+DVLLG ISKNQA S ASNV+ITHENIKG
Sbjct: 269 LKVFNELDALFQEVIDEHLSVGRDKPEQDDIVDVLLGIISKNQAQS-ASNVTITHENIKG 328

Query: 301 VL---------------------------------------------------------- 360
           VL                                                          
Sbjct: 329 VLLNIFLGGLDTSSIIIVWAMAELVKRPKLMKKAQYEIRSHMKSRGKITEKDTEHFQYLK 388

Query: 361 ------LRNHIP------------FRNGRLRFLPKNSGPSQ--CLGN----WTRPQMLER 420
                 LR H P            F      F PK         +G     W  P+    
Sbjct: 389 MIVKETLRMHPPAPLLLPREVMSHFEMEGFDFYPKTMVQINVWAIGRDPKCWKDPEEFV- 448

Query: 421 PRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEE 426
           P R AESCVD+RG HFELLPFGAGRRICPA+NMGIKIVEFALANLLYHFDWKLPEGMKEE
Sbjct: 449 PERFAESCVDFRGHHFELLPFGAGRRICPAMNMGIKIVEFALANLLYHFDWKLPEGMKEE 508

BLAST of HG10015164 vs. NCBI nr
Match: XP_008446135.1 (PREDICTED: cytochrome P450 71B19-like [Cucumis melo] >KAA0034252.1 cytochrome P450 71B19-like [Cucumis melo var. makuwa] >TYK15669.1 cytochrome P450 71B19-like [Cucumis melo var. makuwa])

HSP 1 Score: 568.2 bits (1463), Expect = 6.1e-158
Identity = 315/509 (61.89%), Postives = 363/509 (71.32%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVL-ILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           MSLL LILL SLL+L ILKW KN  +KKGNFPPSPPKLPIIGNLHQLGKLPHKSLW LSQ
Sbjct: 1   MSLLPLILLSSLLLLIILKWMKNRNRKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWHLSQ 60

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
           LYGPIISLNLGGIQT +ISSA AAR  LKTHDLQ+CSRPQ ESARKLTH+F DIAFSPYG
Sbjct: 61  LYGPIISLNLGGIQTIIISSADAARGFLKTHDLQTCSRPQTESARKLTHDFHDIAFSPYG 120

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           DYWRE+R++CV+ELFSLKRLKSY+PI + EM+ LIESISE ASCG  VDLT+K++A TAA
Sbjct: 121 DYWREVRKVCVLELFSLKRLKSYQPIIEHEMNSLIESISESASCGDVVDLTEKSMAFTAA 180

Query: 181 IIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRL 240
           IIFRIAFGKKV  KGDGFHE+VNEAEALLG+YSA ELF    PN VGK I+  +GYQ RL
Sbjct: 181 IIFRIAFGKKVC-KGDGFHEVVNEAEALLGSYSAYELF----PNLVGKAIEWFSGYQKRL 240

Query: 241 HKVFNELNGLFQEVIDEHLCPERD-KPEEDDIIDVLLGISKNQALSAASNVSITHENIKG 300
           +KV+NELN LFQEVIDEHLC  RD + +EDDIIDVLLG+ + Q  SA+SN+SITH++IKG
Sbjct: 241 NKVYNELNSLFQEVIDEHLCVGRDHEAKEDDIIDVLLGLGEKQDQSASSNLSITHDHIKG 300

Query: 301 VLL-----------------------------------RNHIPFRNGR-----------L 360
           +LL                                   R+H+    G            L
Sbjct: 301 ILLNIFLGGLDTSSITIVWAMAELAKKPKLMKKAQQEIRSHMKNNRGNITDKEIEQCQYL 360

Query: 361 RFLPKNS----------GPSQCLGNWTR------PQMLER-------------------- 420
           + + K +           P Q + ++        P+M+ +                    
Sbjct: 361 KMIVKETLRLHPPAPLLLPRQVMSHFEMEGFDFYPKMMVQINAWAIARDPKCWKDPEEFI 420

Query: 421 PRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEE 426
           P R A SC+D+RGQHFELLPFGAGRRICPA+N+GIK VE ALANLLYHFDWKLPEGMKEE
Sbjct: 421 PERFAGSCIDFRGQHFELLPFGAGRRICPAINLGIKNVEVALANLLYHFDWKLPEGMKEE 480

BLAST of HG10015164 vs. NCBI nr
Match: XP_004135499.1 (cytochrome P450 71B19 isoform X2 [Cucumis sativus] >KAE8648692.1 hypothetical protein Csa_008772 [Cucumis sativus])

HSP 1 Score: 558.1 bits (1437), Expect = 6.4e-155
Identity = 311/510 (60.98%), Postives = 352/510 (69.02%), Query Frame = 0

Query: 1   MSLLLLILLFS--LLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLS 60
           M LL LILL +  LL++ILKWKKN  +KKGNFPPSPPKLPIIGNLHQLGKLPH+SLWRLS
Sbjct: 1   MYLLPLILLSTLLLLIIILKWKKNRYRKKGNFPPSPPKLPIIGNLHQLGKLPHQSLWRLS 60

Query: 61  QLYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPY 120
           QLYGPIISL LG IQTT+ISSA AAR L KTHDLQ+CSRPQ E ARKLTHNF D+ FSPY
Sbjct: 61  QLYGPIISLKLGSIQTTIISSADAARGLFKTHDLQTCSRPQTEGARKLTHNFHDLGFSPY 120

Query: 121 GDYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTA 180
           GDYWREMR++CV+ELFSLKR+KSY+ I +QEM+ LIESISE ASCG  VDL+ K++  TA
Sbjct: 121 GDYWREMRKVCVLELFSLKRIKSYQHIIEQEMNSLIESISESASCGDVVDLSDKSMVFTA 180

Query: 181 AIIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTR 240
           AIIFRIAFGKKV  KGDGFHE+VNEAEALLG+YSASELF    PNFVGK ID   GYQ R
Sbjct: 181 AIIFRIAFGKKVC-KGDGFHEVVNEAEALLGSYSASELF----PNFVGKAIDWFNGYQKR 240

Query: 241 LHKVFNELNGLFQEVIDEHLCPERD-KPEEDDIIDVLLGISKNQALSAASNVSITHENIK 300
           L+KV+NEL+GLFQEVIDEHLC  RD + +EDDIIDVLLG+S  Q  SA+ NVSITH++IK
Sbjct: 241 LNKVYNELSGLFQEVIDEHLCVGRDQEAKEDDIIDVLLGLSNQQEQSASFNVSITHDHIK 300

Query: 301 GVL--------------------------------------------------------- 360
           G+L                                                         
Sbjct: 301 GILLSIFLGGLDTSSITIVWAMAELTKKPKLMKKAQQEIRRHMKNRGNITDKEIEQFQYL 360

Query: 361 -------LRNHIP------------FRNGRLRFLPKNSGP--SQCLGN----WTRPQMLE 420
                  LR H P            F+     F PK      +  +G     W  P    
Sbjct: 361 KLIVKETLRMHPPAPLLLPRQVMSHFKMEGFDFYPKTMVQINAWAIGRDPKCWKDPDEF- 420

Query: 421 RPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKE 426
            P R AESC+D+RGQ+FE LPFGAGRRICPA+N+G+K VE ALANLLYHFDWK PEGMKE
Sbjct: 421 MPERFAESCIDFRGQNFEFLPFGAGRRICPAINLGMKNVEVALANLLYHFDWKSPEGMKE 480

BLAST of HG10015164 vs. NCBI nr
Match: XP_031741161.1 (cytochrome P450 71B19 isoform X1 [Cucumis sativus])

HSP 1 Score: 554.7 bits (1428), Expect = 7.0e-154
Identity = 311/519 (59.92%), Postives = 352/519 (67.82%), Query Frame = 0

Query: 1   MSLLLLILLFS--LLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLS 60
           M LL LILL +  LL++ILKWKKN  +KKGNFPPSPPKLPIIGNLHQLGKLPH+SLWRLS
Sbjct: 1   MYLLPLILLSTLLLLIIILKWKKNRYRKKGNFPPSPPKLPIIGNLHQLGKLPHQSLWRLS 60

Query: 61  QLYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPY 120
           QLYGPIISL LG IQTT+ISSA AAR L KTHDLQ+CSRPQ E ARKLTHNF D+ FSPY
Sbjct: 61  QLYGPIISLKLGSIQTTIISSADAARGLFKTHDLQTCSRPQTEGARKLTHNFHDLGFSPY 120

Query: 121 GDYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTA 180
           GDYWREMR++CV+ELFSLKR+KSY+ I +QEM+ LIESISE ASCG  VDL+ K++  TA
Sbjct: 121 GDYWREMRKVCVLELFSLKRIKSYQHIIEQEMNSLIESISESASCGDVVDLSDKSMVFTA 180

Query: 181 AIIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTR 240
           AIIFRIAFGKKV  KGDGFHE+VNEAEALLG+YSASELF    PNFVGK ID   GYQ R
Sbjct: 181 AIIFRIAFGKKVC-KGDGFHEVVNEAEALLGSYSASELF----PNFVGKAIDWFNGYQKR 240

Query: 241 LHKVFNELNGLFQEVIDEHLCPERD-KPEEDDIIDVLLGISKNQALSAASNVSITHENIK 300
           L+KV+NEL+GLFQEVIDEHLC  RD + +EDDIIDVLLG+S  Q  SA+ NVSITH++IK
Sbjct: 241 LNKVYNELSGLFQEVIDEHLCVGRDQEAKEDDIIDVLLGLSNQQEQSASFNVSITHDHIK 300

Query: 301 GVL--------------------------------------------------------- 360
           G+L                                                         
Sbjct: 301 GILLIVLLDMHEQSIFLGGLDTSSITIVWAMAELTKKPKLMKKAQQEIRRHMKNRGNITD 360

Query: 361 ----------------LRNHIP------------FRNGRLRFLPKNSGP--SQCLGN--- 420
                           LR H P            F+     F PK      +  +G    
Sbjct: 361 KEIEQFQYLKLIVKETLRMHPPAPLLLPRQVMSHFKMEGFDFYPKTMVQINAWAIGRDPK 420

Query: 421 -WTRPQMLERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFD 426
            W  P     P R AESC+D+RGQ+FE LPFGAGRRICPA+N+G+K VE ALANLLYHFD
Sbjct: 421 CWKDPDEF-MPERFAESCIDFRGQNFEFLPFGAGRRICPAINLGMKNVEVALANLLYHFD 480

BLAST of HG10015164 vs. NCBI nr
Match: XP_022966918.1 (cytochrome P450 71B34-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 524.6 bits (1350), Expect = 7.8e-145
Identity = 283/504 (56.15%), Postives = 335/504 (66.47%), Query Frame = 0

Query: 4   LLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYGP 63
           L LI+  S L +ILKWK    K+K NFPPSPPK+PIIGNLHQLGKLPHKSLWRLSQLYGP
Sbjct: 23  LHLIIFLSSLFIILKWKAAANKRKCNFPPSPPKIPIIGNLHQLGKLPHKSLWRLSQLYGP 82

Query: 64  IISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYWR 123
           I+SL+LG I+  +ISS   AR LLK HDLQSC+RPQ  + +K ++NFLDIAFSPY DYWR
Sbjct: 83  IMSLSLGRIEIIIISSVETARALLKIHDLQSCNRPQTHAIKKFSYNFLDIAFSPYSDYWR 142

Query: 124 EMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIFR 183
           E+R+IC++E FS+KR+ SY+PIR+QE+   IESIS+ ASCG  VDL++K++ALTA +IFR
Sbjct: 143 EIRKICMLEFFSMKRVLSYKPIREQEVGLFIESISQSASCGAVVDLSEKSIALTAGVIFR 202

Query: 184 IAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRLHKVF 243
           IAFGK+   +GDGFHEL++E EALLG+YSASE FP  VP FVG++ D  +G +TRL +VF
Sbjct: 203 IAFGKQF--EGDGFHELISEVEALLGSYSASEFFP--VP-FVGEVFDWFSGRETRLERVF 262

Query: 244 NELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKGVL--- 303
           NE+NGLFQEVIDEHLCP+R KPE+DDIIDVLL ISKNQ      +V ITHENIK +L   
Sbjct: 263 NEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQV--EPCSVVITHENIKAILFDI 322

Query: 304 ------------------------------------------------------------ 363
                                                                       
Sbjct: 323 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 382

Query: 364 -LRNHIP------------FRNGRLRFLPKNSGPSQ--CLGN----WTRPQMLERPRRVA 423
            LR H P            F+     F PK         +G     W  P+    P R A
Sbjct: 383 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEF-LPERFA 442

Query: 424 ESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEEDLDME 426
           ES +DY+GQHFELLPFGAGRRICP LNMG+K VE ALANLLYH+DWKLP GMKEEDLDME
Sbjct: 443 ESSIDYKGQHFELLPFGAGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDME 502

BLAST of HG10015164 vs. ExPASy Swiss-Prot
Match: Q9LIP6 (Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.9e-87
Identity = 190/509 (37.33%), Postives = 281/509 (55.21%), Query Frame = 0

Query: 2   SLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLY 61
           ++ LL L+F + +L+  +    ++     PPSPP  PIIGNLHQLG+LPH+SLW+LS+ Y
Sbjct: 3   NIWLLSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKY 62

Query: 62  GPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDY 121
           GP++ L LG + T ++SS+  A++ LK HDL  CSRP    AR+L++N+LDIAFSPY DY
Sbjct: 63  GPVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDY 122

Query: 122 WREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAII 181
           W+E+R++ V ELFS K++ S +PI+D+E+  LI+SISE A+   P++L +  +ALT +++
Sbjct: 123 WKEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVV 182

Query: 182 FRIAFGKKVFE----KGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQT 241
            R AF    FE      + F+ +V EA  +LG++SAS+  PY     VG++ID +TG Q 
Sbjct: 183 CRTAFSVN-FEGTVLNSERFNNIVREALEMLGSFSASDFIPY-----VGRIIDLLTGLQG 242

Query: 242 RLHKVFNELNGLFQEVIDEHLCPERDKPEE--DDIIDVLLGISKNQALSAASNVSITHEN 301
           R  +   +L+  ++++ D H    + K EE  +D +D+LL + K +A+    N  +T  +
Sbjct: 243 RRERSMRDLDAFYEQMFDLH----KQKKEEGSEDFVDLLLRLEKEEAV--LGNDKLTRNH 302

Query: 302 IKGVLL-----------------------------------------RNHIPFRN-GRLR 361
           IK +L+                                         +  I F +  +L 
Sbjct: 303 IKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLE 362

Query: 362 FL------------------PKNSGPSQCLGNWTRPQMLE-------------------- 421
           +L                  P+ +     +  +T P                        
Sbjct: 363 YLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEV 422

Query: 422 -RPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMK 424
             P R  ++ +D +GQHFELLPFG GRR+CPA+ MG  +VEF LANLLYHFDWKLPEGMK
Sbjct: 423 FLPERFTDNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMK 482

BLAST of HG10015164 vs. ExPASy Swiss-Prot
Match: Q9LTM4 (Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.0e-86
Identity = 192/508 (37.80%), Postives = 273/508 (53.74%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQL 60
           M++  L +     V ++ + K +K+ K N PPSPPK P+IGNLHQ+G+LPH+SL  L++ 
Sbjct: 1   MAISFLCVFLITFVSLIFFAKKIKRSKWNLPPSPPKFPVIGNLHQIGELPHRSLQHLAER 60

Query: 61  YGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGD 120
           YGP++ L+ G +  TV+SS  AA  +L+THDL  CSRP++   R L+ +F DI F+PYG+
Sbjct: 61  YGPVMLLHFGFVPITVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRDFKDIGFTPYGN 120

Query: 121 YWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAI 180
            W+  R+  + ELF LK+++S+  IR++E ++L++ +SE A    PVDL++    LTA+I
Sbjct: 121 EWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVDRSPVDLSKSLFWLTASI 180

Query: 181 IFRIAFGKKVFEKG----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQ 240
           +FR+A G+   E      +   ELV EAE  L +++ S+ FP      +G L+D  +G  
Sbjct: 181 LFRVALGQNFHESDFIDKEKIEELVFEAETALASFTCSDFFPV---AGLGWLVDWFSGQH 240

Query: 241 TRLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENI 300
            RL+ VF +L+ LFQ VID+HL P R K E +DIID +L +   Q     S++ +T ++I
Sbjct: 241 KRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDVIHKQ--GEDSSLELTIDHI 300

Query: 301 KGVL-----------------------------------LRNHIPFRNGRL--------- 360
           KG L                                   +R  +     R+         
Sbjct: 301 KGFLANIFLAGIDTGAITMIWAVTELVKNPKLIKKVQGDIREQLGSNKERITEEDIEKVP 360

Query: 361 ---------------------------------------RFLPKNSGPSQCLGNWTRPQM 420
                                                  R L   S   +    WT P+ 
Sbjct: 361 YLKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPKE 420

Query: 421 LERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGM 422
            + P R  +S VDYRGQH+ELLPFG+GRRICP + MGI  VE  L NLLY FDWKLP+GM
Sbjct: 421 FD-PERFMDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGM 480

BLAST of HG10015164 vs. ExPASy Swiss-Prot
Match: Q9LIP5 (Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.7e-86
Identity = 193/503 (38.37%), Postives = 279/503 (55.47%), Query Frame = 0

Query: 3   LLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYG 62
           LL LI L  +L+ +   KK+ K ++    P PP  PIIGNLHQ+G+LPH++LW+LS+ YG
Sbjct: 6   LLPLIFLVCILLAVFNHKKHPKYRQF---PCPPGFPIIGNLHQIGELPHQTLWKLSKKYG 65

Query: 63  PIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYW 122
           P++ L LG + T V+SS+  AR++L+ HDL  C+RP +   R+L++N+LDIAFSPY DYW
Sbjct: 66  PVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYW 125

Query: 123 REMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIF 182
           +E+R++CV ELFS K++ S +PI+D+E+  +I+SI+E AS   PV+L  K + LT +++ 
Sbjct: 126 KEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVC 185

Query: 183 RIAFGKKVFE----KGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTR 242
           R AFG   FE      D F+++V EA  +LG++SA++  PY     VG +ID +TG Q R
Sbjct: 186 RTAFGVS-FEGTVLNSDRFNKIVREALEMLGSFSAADFIPY-----VGWIIDVLTGLQGR 245

Query: 243 LHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKG 302
             +   +LN  F+++ D H   E  K   +D +D+LL + K +A+    N  +T  +IK 
Sbjct: 246 RERSKRDLNAFFEQMFDLH--KEGKKEGNEDFVDLLLRLEKEEAV--LGNDKLTRNHIKA 305

Query: 303 VLL-----------------------------------------RNHIPFRN-------- 362
           +LL                                         R+ I F +        
Sbjct: 306 ILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLK 365

Query: 363 ------GRLR-----FLPKNSGPSQCLGNWTRPQMLE---------------------RP 421
                  RL       LP+ +     +  +T P                          P
Sbjct: 366 MVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLP 425

BLAST of HG10015164 vs. ExPASy Swiss-Prot
Match: Q9LTM3 (Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-85
Identity = 190/508 (37.40%), Postives = 267/508 (52.56%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQL 60
           M++  L      L  ++ + K +K  K N PPSPPK P+IGNLHQ+G+LPH+SL  L++ 
Sbjct: 1   MAISFLCFCLITLASLIFFAKKIKHLKWNLPPSPPKFPVIGNLHQIGELPHRSLQHLAER 60

Query: 61  YGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGD 120
           YGP++ L+ G +  TV+SS  AA  +L+THDL  CSRP++   R L+ NF D+ F+PYG+
Sbjct: 61  YGPVMLLHFGFVPVTVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRNFKDVCFTPYGN 120

Query: 121 YWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAI 180
            W+  R+  + ELF LK+++S+  IR++E ++L++ +SE A    PVDL++    LTA+I
Sbjct: 121 EWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVNRSPVDLSKSLFWLTASI 180

Query: 181 IFRIAFGKKVFEKG----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQ 240
            FR+A G+   E      +   ELV EAE  L +++ S+ FP      +G L+D  +G  
Sbjct: 181 FFRVALGQNFHESNFIDKEKIEELVFEAETALASFTCSDFFPV---AGLGWLVDWFSGQH 240

Query: 241 TRLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENI 300
            RL+ VF +L+ LFQ VID+HL P R K E +DIID +L     +   ++  + I H  I
Sbjct: 241 KRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDAIHKEGKDSSLELIIDH--I 300

Query: 301 KGVL-----------------------------------LRNHIPFRNGRL--------- 360
           KG L                                   +R  +     R+         
Sbjct: 301 KGFLANIFLAGIDTGALTMIWAMTELVKNPKLIKKVQGEIREQLGSNKARITEEDIDKVP 360

Query: 361 ---------------------------------------RFLPKNSGPSQCLGNWTRPQM 420
                                                  R L   S   +    WT P+ 
Sbjct: 361 YLKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPEE 420

Query: 421 LERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGM 422
            + P R  +S VDYRGQH+ELLPFG+GRRICP + MGI  VE  L NLLY FDWKLP+GM
Sbjct: 421 FD-PERFMDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGM 480

BLAST of HG10015164 vs. ExPASy Swiss-Prot
Match: O65788 (Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 8.0e-84
Identity = 191/506 (37.75%), Postives = 273/506 (53.95%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILK-WKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           M++LL   L SLL ++   + K  K  K N PPSP  LPIIGNLH L  LPH+   +LS 
Sbjct: 1   MTILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSI 60

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
            YGP++ L LG +   VISS+ AA  +LKT+DL+ CSRP+   + KL++ F DI F+PYG
Sbjct: 61  KYGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYG 120

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           +YWRE+R++ V+ELFS K+++S+  IR++E+ ++++ +SE A    PVDL++   +LTA+
Sbjct: 121 EYWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTAS 180

Query: 181 IIFRIAFGKKVFEKG-----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTG 240
           II R+A G+   E G     D   ELV E+   LGT++ S+ F    P  +G+ +D +  
Sbjct: 181 IICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFF----PGGLGRFVDWLFQ 240

Query: 241 YQTRLHKVFNELNGLFQEVIDEHLCPER--------------DKPEEDD----------- 300
              +++KVF EL+  +Q VID+HL PE               DK E+ D           
Sbjct: 241 RHKKINKVFKELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKA 300

Query: 301 -IIDVLLG---------------ISKNQALSAASNVS-----------ITHENIKGVLLR 360
            ++DV L                + +N  +   +  S           IT E++  V   
Sbjct: 301 IVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYL 360

Query: 361 NHIPFRNGRLRFLPKNSGPSQCLGN---------------------------WTRPQMLE 420
           NHI     RL        P + + +                           W  P+   
Sbjct: 361 NHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEF- 420

Query: 421 RPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKE 422
            P R A S VD+RGQHF+LLPFG+GRRICP + M I  VE AL NLLY+FDW +P+G K 
Sbjct: 421 NPERFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKG 480

BLAST of HG10015164 vs. ExPASy TrEMBL
Match: A0A5D3CZQ9 (Cytochrome P450 71B19-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001660 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 3.0e-158
Identity = 315/509 (61.89%), Postives = 363/509 (71.32%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVL-ILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           MSLL LILL SLL+L ILKW KN  +KKGNFPPSPPKLPIIGNLHQLGKLPHKSLW LSQ
Sbjct: 1   MSLLPLILLSSLLLLIILKWMKNRNRKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWHLSQ 60

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
           LYGPIISLNLGGIQT +ISSA AAR  LKTHDLQ+CSRPQ ESARKLTH+F DIAFSPYG
Sbjct: 61  LYGPIISLNLGGIQTIIISSADAARGFLKTHDLQTCSRPQTESARKLTHDFHDIAFSPYG 120

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           DYWRE+R++CV+ELFSLKRLKSY+PI + EM+ LIESISE ASCG  VDLT+K++A TAA
Sbjct: 121 DYWREVRKVCVLELFSLKRLKSYQPIIEHEMNSLIESISESASCGDVVDLTEKSMAFTAA 180

Query: 181 IIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRL 240
           IIFRIAFGKKV  KGDGFHE+VNEAEALLG+YSA ELF    PN VGK I+  +GYQ RL
Sbjct: 181 IIFRIAFGKKVC-KGDGFHEVVNEAEALLGSYSAYELF----PNLVGKAIEWFSGYQKRL 240

Query: 241 HKVFNELNGLFQEVIDEHLCPERD-KPEEDDIIDVLLGISKNQALSAASNVSITHENIKG 300
           +KV+NELN LFQEVIDEHLC  RD + +EDDIIDVLLG+ + Q  SA+SN+SITH++IKG
Sbjct: 241 NKVYNELNSLFQEVIDEHLCVGRDHEAKEDDIIDVLLGLGEKQDQSASSNLSITHDHIKG 300

Query: 301 VLL-----------------------------------RNHIPFRNGR-----------L 360
           +LL                                   R+H+    G            L
Sbjct: 301 ILLNIFLGGLDTSSITIVWAMAELAKKPKLMKKAQQEIRSHMKNNRGNITDKEIEQCQYL 360

Query: 361 RFLPKNS----------GPSQCLGNWTR------PQMLER-------------------- 420
           + + K +           P Q + ++        P+M+ +                    
Sbjct: 361 KMIVKETLRLHPPAPLLLPRQVMSHFEMEGFDFYPKMMVQINAWAIARDPKCWKDPEEFI 420

Query: 421 PRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEE 426
           P R A SC+D+RGQHFELLPFGAGRRICPA+N+GIK VE ALANLLYHFDWKLPEGMKEE
Sbjct: 421 PERFAGSCIDFRGQHFELLPFGAGRRICPAINLGIKNVEVALANLLYHFDWKLPEGMKEE 480

BLAST of HG10015164 vs. ExPASy TrEMBL
Match: A0A1S3BF10 (cytochrome P450 71B19-like OS=Cucumis melo OX=3656 GN=LOC103488949 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 3.0e-158
Identity = 315/509 (61.89%), Postives = 363/509 (71.32%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVL-ILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           MSLL LILL SLL+L ILKW KN  +KKGNFPPSPPKLPIIGNLHQLGKLPHKSLW LSQ
Sbjct: 1   MSLLPLILLSSLLLLIILKWMKNRNRKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWHLSQ 60

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
           LYGPIISLNLGGIQT +ISSA AAR  LKTHDLQ+CSRPQ ESARKLTH+F DIAFSPYG
Sbjct: 61  LYGPIISLNLGGIQTIIISSADAARGFLKTHDLQTCSRPQTESARKLTHDFHDIAFSPYG 120

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           DYWRE+R++CV+ELFSLKRLKSY+PI + EM+ LIESISE ASCG  VDLT+K++A TAA
Sbjct: 121 DYWREVRKVCVLELFSLKRLKSYQPIIEHEMNSLIESISESASCGDVVDLTEKSMAFTAA 180

Query: 181 IIFRIAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRL 240
           IIFRIAFGKKV  KGDGFHE+VNEAEALLG+YSA ELF    PN VGK I+  +GYQ RL
Sbjct: 181 IIFRIAFGKKVC-KGDGFHEVVNEAEALLGSYSAYELF----PNLVGKAIEWFSGYQKRL 240

Query: 241 HKVFNELNGLFQEVIDEHLCPERD-KPEEDDIIDVLLGISKNQALSAASNVSITHENIKG 300
           +KV+NELN LFQEVIDEHLC  RD + +EDDIIDVLLG+ + Q  SA+SN+SITH++IKG
Sbjct: 241 NKVYNELNSLFQEVIDEHLCVGRDHEAKEDDIIDVLLGLGEKQDQSASSNLSITHDHIKG 300

Query: 301 VLL-----------------------------------RNHIPFRNGR-----------L 360
           +LL                                   R+H+    G            L
Sbjct: 301 ILLNIFLGGLDTSSITIVWAMAELAKKPKLMKKAQQEIRSHMKNNRGNITDKEIEQCQYL 360

Query: 361 RFLPKNS----------GPSQCLGNWTR------PQMLER-------------------- 420
           + + K +           P Q + ++        P+M+ +                    
Sbjct: 361 KMIVKETLRLHPPAPLLLPRQVMSHFEMEGFDFYPKMMVQINAWAIARDPKCWKDPEEFI 420

Query: 421 PRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEE 426
           P R A SC+D+RGQHFELLPFGAGRRICPA+N+GIK VE ALANLLYHFDWKLPEGMKEE
Sbjct: 421 PERFAGSCIDFRGQHFELLPFGAGRRICPAINLGIKNVEVALANLLYHFDWKLPEGMKEE 480

BLAST of HG10015164 vs. ExPASy TrEMBL
Match: A0A6J1HTM0 (cytochrome P450 71B34-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466484 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.8e-145
Identity = 283/504 (56.15%), Postives = 335/504 (66.47%), Query Frame = 0

Query: 4   LLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYGP 63
           L LI+  S L +ILKWK    K+K NFPPSPPK+PIIGNLHQLGKLPHKSLWRLSQLYGP
Sbjct: 23  LHLIIFLSSLFIILKWKAAANKRKCNFPPSPPKIPIIGNLHQLGKLPHKSLWRLSQLYGP 82

Query: 64  IISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYWR 123
           I+SL+LG I+  +ISS   AR LLK HDLQSC+RPQ  + +K ++NFLDIAFSPY DYWR
Sbjct: 83  IMSLSLGRIEIIIISSVETARALLKIHDLQSCNRPQTHAIKKFSYNFLDIAFSPYSDYWR 142

Query: 124 EMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIFR 183
           E+R+IC++E FS+KR+ SY+PIR+QE+   IESIS+ ASCG  VDL++K++ALTA +IFR
Sbjct: 143 EIRKICMLEFFSMKRVLSYKPIREQEVGLFIESISQSASCGAVVDLSEKSIALTAGVIFR 202

Query: 184 IAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRLHKVF 243
           IAFGK+   +GDGFHEL++E EALLG+YSASE FP  VP FVG++ D  +G +TRL +VF
Sbjct: 203 IAFGKQF--EGDGFHELISEVEALLGSYSASEFFP--VP-FVGEVFDWFSGRETRLERVF 262

Query: 244 NELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKGVL--- 303
           NE+NGLFQEVIDEHLCP+R KPE+DDIIDVLL ISKNQ      +V ITHENIK +L   
Sbjct: 263 NEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQV--EPCSVVITHENIKAILFDI 322

Query: 304 ------------------------------------------------------------ 363
                                                                       
Sbjct: 323 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 382

Query: 364 -LRNHIP------------FRNGRLRFLPKNSGPSQ--CLGN----WTRPQMLERPRRVA 423
            LR H P            F+     F PK         +G     W  P+    P R A
Sbjct: 383 TLRLHPPAPLLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEF-LPERFA 442

Query: 424 ESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEEDLDME 426
           ES +DY+GQHFELLPFGAGRRICP LNMG+K VE ALANLLYH+DWKLP GMKEEDLDME
Sbjct: 443 ESSIDYKGQHFELLPFGAGRRICPGLNMGVKTVELALANLLYHYDWKLPNGMKEEDLDME 502

BLAST of HG10015164 vs. ExPASy TrEMBL
Match: A0A6J1HTN1 (cytochrome P450 71B34-like OS=Cucurbita maxima OX=3661 GN=LOC111466493 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.9e-145
Identity = 283/503 (56.26%), Postives = 334/503 (66.40%), Query Frame = 0

Query: 4   LLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYGP 63
           L LI+  S L +ILKWK    K+K NFPPSPPK+PIIGNLHQLGKLPHKSLWRLSQLYGP
Sbjct: 7   LHLIIFLSSLFIILKWKAAANKRKCNFPPSPPKIPIIGNLHQLGKLPHKSLWRLSQLYGP 66

Query: 64  IISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYWR 123
           I+SL+LG I+  +ISS   AR LLK HDLQSC+RPQ  + +K ++NFLDIAFSP  DYWR
Sbjct: 67  IMSLSLGRIEIIIISSVETARALLKNHDLQSCNRPQTHAIKKFSYNFLDIAFSPCSDYWR 126

Query: 124 EMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIFR 183
           E+R+IC++E FS+KR+ SYEPIR+QE+   IESIS+ ASCG  VDL++K++ALT  +IFR
Sbjct: 127 EIRKICMLEFFSMKRVLSYEPIREQEVGLFIESISQSASCGAVVDLSEKSIALTTGVIFR 186

Query: 184 IAFGKKVFEKGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTRLHKVF 243
           IAFGK+   +GDGFHEL++E EALLG+YSASE FP  VP FVG++ D  +G +TRL +VF
Sbjct: 187 IAFGKQF--EGDGFHELISEVEALLGSYSASEFFP--VP-FVGEVFDWFSGRETRLERVF 246

Query: 244 NELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKGVL--- 303
           NE+NGLFQEVIDEHLCP+R KPE+DDIIDVLL ISKNQ      +V ITHENIK +L   
Sbjct: 247 NEMNGLFQEVIDEHLCPQRPKPEQDDIIDVLLAISKNQV--EPCSVVITHENIKAILFDI 306

Query: 304 ------------------------------------------------------------ 363
                                                                       
Sbjct: 307 FLAGLDTSSITIVWAMAELAKNSKLMKKAQEEIRNYVGNKRKVTEKDVEELPYLKMIVKE 366

Query: 364 -LRNHIP-----------FRNGRLRFLPKNSGPSQ--CLGN----WTRPQMLERPRRVAE 423
            LR H P           F+     F PK         +G     W  P+    P R AE
Sbjct: 367 TLRLHPPAPLLPRETISHFKIEDYNFYPKTMVQVNVWAIGRDPTYWKDPEEF-LPERFAE 426

Query: 424 SCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKEEDLDMEE 426
           S +DY+GQHFELLPFGAGRRICP LNMG+K VEFALANLLYH+DWKLP GMKEEDLDMEE
Sbjct: 427 SSIDYKGQHFELLPFGAGRRICPGLNMGVKTVEFALANLLYHYDWKLPNGMKEEDLDMEE 486

BLAST of HG10015164 vs. ExPASy TrEMBL
Match: A0A6J1DE04 (cytochrome P450 71B34-like OS=Momordica charantia OX=3673 GN=LOC111019659 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 4.2e-144
Identity = 292/506 (57.71%), Postives = 336/506 (66.40%), Query Frame = 0

Query: 4   LLLILLFSLLVLILKWK--KNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLY 63
           L L +  SLL+ ++KWK   + ++K+G FPPSPPKLPIIGNLHQLG LPHKSLWRLSQ Y
Sbjct: 67  LPLFIFLSLLIFLIKWKTSSSSRRKRGRFPPSPPKLPIIGNLHQLGNLPHKSLWRLSQRY 126

Query: 64  GPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDY 123
           GPI+ L LGG++TT+ISSA AAR LLKTHDL SCSRPQ  S RKLT+NF DIAFSPYGDY
Sbjct: 127 GPIMGLTLGGVETTIISSADAARALLKTHDLDSCSRPQSHSVRKLTYNFRDIAFSPYGDY 186

Query: 124 WREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAII 183
           WRE+R+ICV+ELFS+KR+KSYEPIR+QE+  LIESI+E AS   PVDLT K+VALTAAI+
Sbjct: 187 WREIRKICVLELFSVKRVKSYEPIREQEIGSLIESIAEFASSATPVDLTDKSVALTAAIV 246

Query: 184 FRIAFGKKVFEKGD----GFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQT 243
           FRIAFGK+ FE GD     FHELV+E EA+LG+YSASE   Y+   FVGKLID ++G   
Sbjct: 247 FRIAFGKR-FEGGDFRGGRFHELVSEVEAMLGSYSASE---YFAVPFVGKLIDRISGRHG 306

Query: 244 RLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIK 303
           RL +VFNEL+ LFQEVIDEHL PER K EEDDIIDVLLGISK Q  S   +V+I HENIK
Sbjct: 307 RLERVFNELDALFQEVIDEHLRPERIKAEEDDIIDVLLGISKQQHQS--GSVAIAHENIK 366

Query: 304 GVLL-----------------------------------RNHI--------------PFR 363
            +LL                                   R H+              P+ 
Sbjct: 367 AILLNIFLGGLDTGSITIVWAMAELAKKPKLMKKAQQEIRKHVGSKGKLSDSDTEELPYL 426

Query: 364 NGRLRFLPKNSGPSQCL---------------------------------GNWTRPQMLE 422
              ++   +   P+  L                                  +W  P+   
Sbjct: 427 KMMVKETLRLHPPAPLLLPRETMSHFQIQGYDFWPKTVVQVNAWAIGRDPTSWKDPEEF- 486

BLAST of HG10015164 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 322.8 bits (826), Expect = 4.2e-88
Identity = 190/509 (37.33%), Postives = 281/509 (55.21%), Query Frame = 0

Query: 2   SLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLY 61
           ++ LL L+F + +L+  +    ++     PPSPP  PIIGNLHQLG+LPH+SLW+LS+ Y
Sbjct: 3   NIWLLSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKY 62

Query: 62  GPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDY 121
           GP++ L LG + T ++SS+  A++ LK HDL  CSRP    AR+L++N+LDIAFSPY DY
Sbjct: 63  GPVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDY 122

Query: 122 WREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAII 181
           W+E+R++ V ELFS K++ S +PI+D+E+  LI+SISE A+   P++L +  +ALT +++
Sbjct: 123 WKEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVV 182

Query: 182 FRIAFGKKVFE----KGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQT 241
            R AF    FE      + F+ +V EA  +LG++SAS+  PY     VG++ID +TG Q 
Sbjct: 183 CRTAFSVN-FEGTVLNSERFNNIVREALEMLGSFSASDFIPY-----VGRIIDLLTGLQG 242

Query: 242 RLHKVFNELNGLFQEVIDEHLCPERDKPEE--DDIIDVLLGISKNQALSAASNVSITHEN 301
           R  +   +L+  ++++ D H    + K EE  +D +D+LL + K +A+    N  +T  +
Sbjct: 243 RRERSMRDLDAFYEQMFDLH----KQKKEEGSEDFVDLLLRLEKEEAV--LGNDKLTRNH 302

Query: 302 IKGVLL-----------------------------------------RNHIPFRN-GRLR 361
           IK +L+                                         +  I F +  +L 
Sbjct: 303 IKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLE 362

Query: 362 FL------------------PKNSGPSQCLGNWTRPQMLE-------------------- 421
           +L                  P+ +     +  +T P                        
Sbjct: 363 YLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEV 422

Query: 422 -RPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMK 424
             P R  ++ +D +GQHFELLPFG GRR+CPA+ MG  +VEF LANLLYHFDWKLPEGMK
Sbjct: 423 FLPERFTDNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMK 482

BLAST of HG10015164 vs. TAIR 10
Match: AT3G26170.1 (cytochrome P450, family 71, subfamily B, polypeptide 19 )

HSP 1 Score: 322.0 bits (824), Expect = 7.1e-88
Identity = 192/508 (37.80%), Postives = 273/508 (53.74%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQL 60
           M++  L +     V ++ + K +K+ K N PPSPPK P+IGNLHQ+G+LPH+SL  L++ 
Sbjct: 1   MAISFLCVFLITFVSLIFFAKKIKRSKWNLPPSPPKFPVIGNLHQIGELPHRSLQHLAER 60

Query: 61  YGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGD 120
           YGP++ L+ G +  TV+SS  AA  +L+THDL  CSRP++   R L+ +F DI F+PYG+
Sbjct: 61  YGPVMLLHFGFVPITVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRDFKDIGFTPYGN 120

Query: 121 YWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAI 180
            W+  R+  + ELF LK+++S+  IR++E ++L++ +SE A    PVDL++    LTA+I
Sbjct: 121 EWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVDRSPVDLSKSLFWLTASI 180

Query: 181 IFRIAFGKKVFEKG----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQ 240
           +FR+A G+   E      +   ELV EAE  L +++ S+ FP      +G L+D  +G  
Sbjct: 181 LFRVALGQNFHESDFIDKEKIEELVFEAETALASFTCSDFFPV---AGLGWLVDWFSGQH 240

Query: 241 TRLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENI 300
            RL+ VF +L+ LFQ VID+HL P R K E +DIID +L +   Q     S++ +T ++I
Sbjct: 241 KRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDVIHKQ--GEDSSLELTIDHI 300

Query: 301 KGVL-----------------------------------LRNHIPFRNGRL--------- 360
           KG L                                   +R  +     R+         
Sbjct: 301 KGFLANIFLAGIDTGAITMIWAVTELVKNPKLIKKVQGDIREQLGSNKERITEEDIEKVP 360

Query: 361 ---------------------------------------RFLPKNSGPSQCLGNWTRPQM 420
                                                  R L   S   +    WT P+ 
Sbjct: 361 YLKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPKE 420

Query: 421 LERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGM 422
            + P R  +S VDYRGQH+ELLPFG+GRRICP + MGI  VE  L NLLY FDWKLP+GM
Sbjct: 421 FD-PERFMDSFVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGM 480

BLAST of HG10015164 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-87
Identity = 193/503 (38.37%), Postives = 279/503 (55.47%), Query Frame = 0

Query: 3   LLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQLYG 62
           LL LI L  +L+ +   KK+ K ++    P PP  PIIGNLHQ+G+LPH++LW+LS+ YG
Sbjct: 6   LLPLIFLVCILLAVFNHKKHPKYRQF---PCPPGFPIIGNLHQIGELPHQTLWKLSKKYG 65

Query: 63  PIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGDYW 122
           P++ L LG + T V+SS+  AR++L+ HDL  C+RP +   R+L++N+LDIAFSPY DYW
Sbjct: 66  PVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYW 125

Query: 123 REMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAIIF 182
           +E+R++CV ELFS K++ S +PI+D+E+  +I+SI+E AS   PV+L  K + LT +++ 
Sbjct: 126 KEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVC 185

Query: 183 RIAFGKKVFE----KGDGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQTR 242
           R AFG   FE      D F+++V EA  +LG++SA++  PY     VG +ID +TG Q R
Sbjct: 186 RTAFGVS-FEGTVLNSDRFNKIVREALEMLGSFSAADFIPY-----VGWIIDVLTGLQGR 245

Query: 243 LHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENIKG 302
             +   +LN  F+++ D H   E  K   +D +D+LL + K +A+    N  +T  +IK 
Sbjct: 246 RERSKRDLNAFFEQMFDLH--KEGKKEGNEDFVDLLLRLEKEEAV--LGNDKLTRNHIKA 305

Query: 303 VLL-----------------------------------------RNHIPFRN-------- 362
           +LL                                         R+ I F +        
Sbjct: 306 ILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLK 365

Query: 363 ------GRLR-----FLPKNSGPSQCLGNWTRPQMLE---------------------RP 421
                  RL       LP+ +     +  +T P                          P
Sbjct: 366 MVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLP 425

BLAST of HG10015164 vs. TAIR 10
Match: AT3G26180.1 (cytochrome P450, family 71, subfamily B, polypeptide 20 )

HSP 1 Score: 317.4 bits (812), Expect = 1.8e-86
Identity = 190/508 (37.40%), Postives = 267/508 (52.56%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILKWKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQL 60
           M++  L      L  ++ + K +K  K N PPSPPK P+IGNLHQ+G+LPH+SL  L++ 
Sbjct: 1   MAISFLCFCLITLASLIFFAKKIKHLKWNLPPSPPKFPVIGNLHQIGELPHRSLQHLAER 60

Query: 61  YGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYGD 120
           YGP++ L+ G +  TV+SS  AA  +L+THDL  CSRP++   R L+ NF D+ F+PYG+
Sbjct: 61  YGPVMLLHFGFVPVTVVSSREAAEEVLRTHDLDCCSRPKLVGTRLLSRNFKDVCFTPYGN 120

Query: 121 YWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAAI 180
            W+  R+  + ELF LK+++S+  IR++E ++L++ +SE A    PVDL++    LTA+I
Sbjct: 121 EWKARRKFALRELFCLKKVQSFRHIREEECNFLVKQLSESAVNRSPVDLSKSLFWLTASI 180

Query: 181 IFRIAFGKKVFEKG----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTGYQ 240
            FR+A G+   E      +   ELV EAE  L +++ S+ FP      +G L+D  +G  
Sbjct: 181 FFRVALGQNFHESNFIDKEKIEELVFEAETALASFTCSDFFPV---AGLGWLVDWFSGQH 240

Query: 241 TRLHKVFNELNGLFQEVIDEHLCPERDKPEEDDIIDVLLGISKNQALSAASNVSITHENI 300
            RL+ VF +L+ LFQ VID+HL P R K E +DIID +L     +   ++  + I H  I
Sbjct: 241 KRLNDVFYKLDALFQHVIDDHLNPGRSK-EHEDIIDSMLDAIHKEGKDSSLELIIDH--I 300

Query: 301 KGVL-----------------------------------LRNHIPFRNGRL--------- 360
           KG L                                   +R  +     R+         
Sbjct: 301 KGFLANIFLAGIDTGALTMIWAMTELVKNPKLIKKVQGEIREQLGSNKARITEEDIDKVP 360

Query: 361 ---------------------------------------RFLPKNSGPSQCLGNWTRPQM 420
                                                  R L   S   +    WT P+ 
Sbjct: 361 YLKMVIKETFRLHPAAPLILPRETMAHIKVQGYDIPPKRRILVNVSAIGRDPKLWTNPEE 420

Query: 421 LERPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGM 422
            + P R  +S VDYRGQH+ELLPFG+GRRICP + MGI  VE  L NLLY FDWKLP+GM
Sbjct: 421 FD-PERFMDSSVDYRGQHYELLPFGSGRRICPGMPMGIAAVELGLLNLLYFFDWKLPDGM 480

BLAST of HG10015164 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 312.4 bits (799), Expect = 5.7e-85
Identity = 191/506 (37.75%), Postives = 273/506 (53.95%), Query Frame = 0

Query: 1   MSLLLLILLFSLLVLILK-WKKNVKKKKGNFPPSPPKLPIIGNLHQLGKLPHKSLWRLSQ 60
           M++LL   L SLL ++   + K  K  K N PPSP  LPIIGNLH L  LPH+   +LS 
Sbjct: 1   MTILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSI 60

Query: 61  LYGPIISLNLGGIQTTVISSAYAARRLLKTHDLQSCSRPQIESARKLTHNFLDIAFSPYG 120
            YGP++ L LG +   VISS+ AA  +LKT+DL+ CSRP+   + KL++ F DI F+PYG
Sbjct: 61  KYGPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYG 120

Query: 121 DYWREMREICVVELFSLKRLKSYEPIRDQEMSWLIESISEVASCGGPVDLTQKTVALTAA 180
           +YWRE+R++ V+ELFS K+++S+  IR++E+ ++++ +SE A    PVDL++   +LTA+
Sbjct: 121 EYWREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTAS 180

Query: 181 IIFRIAFGKKVFEKG-----DGFHELVNEAEALLGTYSASELFPYYVPNFVGKLIDCVTG 240
           II R+A G+   E G     D   ELV E+   LGT++ S+ F    P  +G+ +D +  
Sbjct: 181 IICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFF----PGGLGRFVDWLFQ 240

Query: 241 YQTRLHKVFNELNGLFQEVIDEHLCPER--------------DKPEEDD----------- 300
              +++KVF EL+  +Q VID+HL PE               DK E+ D           
Sbjct: 241 RHKKINKVFKELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKA 300

Query: 301 -IIDVLLG---------------ISKNQALSAASNVS-----------ITHENIKGVLLR 360
            ++DV L                + +N  +   +  S           IT E++  V   
Sbjct: 301 IVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYL 360

Query: 361 NHIPFRNGRLRFLPKNSGPSQCLGN---------------------------WTRPQMLE 420
           NHI     RL        P + + +                           W  P+   
Sbjct: 361 NHILKETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEF- 420

Query: 421 RPRRVAESCVDYRGQHFELLPFGAGRRICPALNMGIKIVEFALANLLYHFDWKLPEGMKE 422
            P R A S VD+RGQHF+LLPFG+GRRICP + M I  VE AL NLLY+FDW +P+G K 
Sbjct: 421 NPERFANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKG 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891462.16.8e-16565.62cytochrome P450 71B2-like [Benincasa hispida][more]
XP_008446135.16.1e-15861.89PREDICTED: cytochrome P450 71B19-like [Cucumis melo] >KAA0034252.1 cytochrome P4... [more]
XP_004135499.16.4e-15560.98cytochrome P450 71B19 isoform X2 [Cucumis sativus] >KAE8648692.1 hypothetical pr... [more]
XP_031741161.17.0e-15459.92cytochrome P450 71B19 isoform X1 [Cucumis sativus][more]
XP_022966918.17.8e-14556.15cytochrome P450 71B34-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LIP65.9e-8737.33Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1[more]
Q9LTM41.0e-8637.80Cytochrome P450 71B19 OS=Arabidopsis thaliana OX=3702 GN=CYP71B19 PE=1 SV=1[more]
Q9LIP51.7e-8638.37Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1[more]
Q9LTM32.5e-8537.40Cytochrome P450 71B20 OS=Arabidopsis thaliana OX=3702 GN=CYP71B20 PE=2 SV=1[more]
O657888.0e-8437.75Cytochrome P450 71B2 OS=Arabidopsis thaliana OX=3702 GN=CYP71B2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CZQ93.0e-15861.89Cytochrome P450 71B19-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BF103.0e-15861.89cytochrome P450 71B19-like OS=Cucumis melo OX=3656 GN=LOC103488949 PE=3 SV=1[more]
A0A6J1HTM03.8e-14556.15cytochrome P450 71B34-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146648... [more]
A0A6J1HTN14.9e-14556.26cytochrome P450 71B34-like OS=Cucurbita maxima OX=3661 GN=LOC111466493 PE=3 SV=1[more]
A0A6J1DE044.2e-14457.71cytochrome P450 71B34-like OS=Momordica charantia OX=3673 GN=LOC111019659 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G26300.14.2e-8837.33cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26170.17.1e-8837.80cytochrome P450, family 71, subfamily B, polypeptide 19 [more]
AT3G26310.11.2e-8738.37cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G26180.11.8e-8637.40cytochrome P450, family 71, subfamily B, polypeptide 20 [more]
AT1G13080.15.7e-8537.75cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 58..77
score: 31.59
coord: 82..103
score: 20.29
coord: 176..194
score: 22.97
coord: 354..364
score: 59.28
coord: 364..387
score: 22.57
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 312..423
e-value: 3.5E-13
score: 51.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 15..303
e-value: 9.5E-46
score: 158.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 29..420
IPR001128Cytochrome P450PFAMPF00067p450coord: 348..409
e-value: 6.9E-9
score: 35.0
coord: 31..297
e-value: 1.0E-25
score: 90.4
NoneNo IPR availablePANTHERPTHR47956:SF5CYTOCHROME P450 71B16-RELATEDcoord: 4..301
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 328..422
coord: 4..301
NoneNo IPR availablePANTHERPTHR47956:SF5CYTOCHROME P450 71B16-RELATEDcoord: 328..422
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 357..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10015164.1HG10015164.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen