HG10015103 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10015103
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	alpha-ketoglutarate-dependent dioxygenase alkB
Location	Chr02: 23902146 .. 23903870 (+)
RNA-Seq Expression	HG10015103
Synteny	HG10015103

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGTATTCCTTCACAATTGCTCTTTCAGGTTCTATGTCATTCTATTGCCATTGAATTCCTTGCAAGAGACGATGTTTCAAACTCTCTTTTTTGCGATTTCTGTCAGGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGTATTGCCGATTCTCCATTTTCGGCTCTGTTTCAAAGAAAAAGTTCCCAATTCAGCTGTTCTTTATGTGTTTAACATGTAGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGGTGAGTTCAACTATAAAACTCCATAGTTTGCTCGTATTATACCAATTTAAACTCTGTTTTGAATGTTTTGAATGGTTCCTATTTGAAAGTTGTATTTTATGAGACTATTCCAAAACGCTAAAACCATCATAGTATTGTCTCTGTTGCTATGTTTTGATATGTTATTTTCTGAATTATTCACCTTGTTATGGCGTAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTAACTAGAGAGAGTTAACAAAATTTTGCCATTGTCGAGGATTCTACATTGGAAACATTAGGACTCACAATGTTTATTAGATAGCTACTCCTCTCGGCTCTCGCCAATTGATTTTAAGATGAAATCCTTGTTAGTTAATAGCCATGACGCTGACACGTTATCGATATTTCCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGGTATCCTGCTAACTTATCCTCTTGCAATTGCATTTCTTTCTGATATTTAAAAGTACATGGGCTGATACTGATTCTGTTATCTATTATATCTGCTCGCCTCCACTTTGTCCAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

mRNA sequence

ATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

Coding sequence (CDS)

ATGTACGGATCCGACCAAGGTATCGACGATTCCGAGCGCACCGCTTTCAGAAGAGCAGAAAAGAAATACAAATTGTACTACGACGACACCTACAAATCTTCGAAAAAGAAAAAACTACCGAAACAAGTCGATTTGTCTGAGGTTATCGATTTCAAGTGCATCCTCGAATGTTACAAACAAGATGGTGCACTTCCGCTGGGCGTGTACGCGACTAAGTGCGATCTCGATGGGCCAGTCTTCTGCTTGGAGAATCGTCCTGGGTTTTATTTTATTCCTGGAGCGTTGAGCTTACAAGAGCAATGCCAATGGATCAGGGAGAGTTTAACGAATTTCCCGCAGCCTCCTAACAGAACCAACCACAATGCTATTTATGGACCAATTAAAGACTTGTTCATTGCAGCAAAGGGAAAGAAAGTTTTAGTTGAAGATGATGAAATCTCTGATTTCAACGTTGATTCTGATGTTGAACCTTCTGTTAGCAATGGAAGTACTCATAACTGGAAGTTTGTGGAGGAGCATACTGTTTCATCCAGAAGAGGGTCGGCCTGCAAATCAATTCCTGCTTCAGTATTACTTCGAAAGTTGCGTTGGAGTACACTCGGCCTACAATTTGATTGGTCCAAGCGAAGCTATGACATATCTCTGCCCCATAATAAGATACCCTCTGCCCTTTGTCAACTCGCCAAAAGAATGGCGGCAGCTGCAATGCCAACTGGGGAAGAATTCAAGCCTGAAGCTGCAATAGTTAATTATTTTGCTTCAGGTGACACGCTTGGTGGTCACCTAGATGACATGGAAGTGGACTGGAGCAAGCCAATTGTTAGCATGAGTTTGGGTTGCAAAGCTATTTTTCTTTTGGGTGGTAAGTCGAGACAGGATCCACCCGTAGCCATGTTTCTTCGAAGCGGAGATGTTGTGCTAATGGCTGGAGAAGCAAGGGAATGTTTCCATGGTAATGTCGCCTTAATGTTCACTCTTACATCATATCAAAATGTATTTCTATATCAGATACCTGCACTTGCATTGTCCTTTTGTTTTGTTATCACCTTGGATATCTGA

Protein sequence

MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQIPALALSFCFVITLDI

Homology

[Close]

BLAST of HG10015103 vs. NCBI nr
Match: XP_038891364.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida])

HSP 1 Score: 622.9 bits (1605), Expect = 1.7e-174
Identity = 301/318 (94.65%), Postives = 308/318 (96.86%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+  DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCIL CYKQ
Sbjct: 1   MYGSDKVTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILGCYKQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLGV A KCDLD PVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQP NRTNH
Sbjct: 61  DGALPLGVNAIKCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPSNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYG I+DLFIAAKGKKVLVEDDEISDFNVDSDVE SVSNG+THNWKFVEE+TVSS+RG
Sbjct: 121 NAIYGSIQDLFIAAKGKKVLVEDDEISDFNVDSDVERSVSNGNTHNWKFVEENTVSSKRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +ACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]

BLAST of HG10015103 vs. NCBI nr
Match: XP_008446031.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [Cucumis melo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 294/318 (92.45%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE YKQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLD PVFCLENRPGFYFIPGALSLQEQCQWIRESL +FPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK KKVLVE DEISDFN+DSDVEPS+SNGSTHNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +ACKSI ASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]

BLAST of HG10015103 vs. NCBI nr
Match: XP_004135327.1 (alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 292/318 (91.82%), Postives = 304/318 (95.60%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK  KVLVE DEISDF +DSDVEPS+SNG+THNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +A KSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]

BLAST of HG10015103 vs. NCBI nr
Match: XP_022945036.1 (alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata])

HSP 1 Score: 597.4 bits (1539), Expect = 7.8e-167
Identity = 292/336 (86.90%), Postives = 311/336 (92.56%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFK ILECY Q
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLGV ATKCDLDGPVFCLENRPGFYFIPGALSL+EQCQWIRESL +FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK K+VLVED+EIS  NVDSD EPS+ NG+++ WKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           + CKS+PAS LLRKLRWSTLGLQFDWSKRSYDISLPHN+IPSALCQL KRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQ 337
           RSGDVVLMAGEARECFHG V  +FT    + + L +
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFTDEESEEISLLE 334

[Close]

BLAST of HG10015103 vs. NCBI nr
Match: KAG6573589.1 (Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 596.7 bits (1537), Expect = 1.3e-166
Identity = 292/336 (86.90%), Postives = 310/336 (92.26%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFK I ECY Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRIFECYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLGV ATKCDLDGPVFCLENRPGFYFIPGALSL+EQCQWIR+SL +FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRKSLKDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK K+VLVED+EIS  NVDSD EPSV NG+++ WKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           + CKS+PAS LLRKLRWSTLGLQFDWSKRSYDISLPHN+IPSALCQL KRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQ 337
           RSGDVVLMAGEARECFHG V  +FT    + + L +
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFTDEESEEISLLE 334

[Close]

BLAST of HG10015103 vs. ExPASy Swiss-Prot
Match: Q9SA98 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 GN=At1g11780 PE=2 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.2e-125
Identity = 219/318 (68.87%), Postives = 248/318 (77.99%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MY S    DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLT+FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLF +AK  KVLV+DD                  + + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           S+CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 300

[Close]

BLAST of HG10015103 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.2e-38
Identity = 104/335 (31.04%), Postives = 165/335 (49.25%), Query Frame = 0


Query: 14  TAFRRAEKKYKLYYDDTYKSSKKKKLPKQ----VDLSEVIDF-----------KCILEC- 73
           T F R ++ ++       KS+  K +PK+    +D S V+DF           K I++C 
Sbjct: 36  TEFMRVQRLFR----QVTKSANGKNIPKEKREPIDYSPVLDFHNLENNTEENKKLIIDCT 95

Query: 74  ---------YKQDGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL 133
                    + +D    L     K      V+ L+  PGFYFI    +  +Q +WI+ +L
Sbjct: 96  SNVTTHDFEFNRDTEFYLHPREWK------VYGLQGYPGFYFIKSPFTASQQKKWIKHAL 155

Query: 134 TNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLVEDD-----EISDFNVDSDVEPSVSNGS 193
            ++  PPN  N    +GPIK+L+    G+K L+ ++     +  D  ++    P   NG 
Sbjct: 156 EDYADPPNNNNITLFHGPIKNLW--KNGEKELINEELKSQGKHDDDEIEQPTRPLDKNG- 215

Query: 194 THNWKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSA 253
                   E   + R+           LL KL WSTLG Q+ W+ R Y     + + P  
Sbjct: 216 --------EPLPTYRQ-----------LLDKLAWSTLGYQYQWTPRLYSEEF-YEEFPDD 275

Query: 254 LCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAI 313
           L +L +++A A     + +  EAA VN+++    +GGHLDD E +  KPI+S+S G  A+
Sbjct: 276 LQELVQKIAIAT--KFDPYVAEAATVNFYSEDSIMGGHLDDAEQEMEKPIISISFGSTAV 335

Query: 314 FLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHG 319
           FL+G ++R   PV +F+RSGD+V+M G +R C+HG
Sbjct: 336 FLMGAETRDIAPVPLFIRSGDIVIMGGRSRYCYHG 335

[Close]

BLAST of HG10015103 vs. ExPASy Swiss-Prot
Match: Q13686 (Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 2.2e-26
Identity = 79/237 (33.33%), Postives = 114/237 (48.10%), Query Frame = 0


Query: 82  LENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLV 141
           L+  PGF FIP       Q  W+++ L  + Q PN  N                      
Sbjct: 93  LKGYPGFIFIPNPFLPGYQWHWVKQCLKLYSQKPNVCN---------------------- 152

Query: 142 EDDEISDFNVDSDVEPSVSNGSTHN-WKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTL 201
                        ++  +S   T + W+  +E     R   A K  P S LL KLRW T+
Sbjct: 153 -------------LDKHMSKEETQDLWEQSKEFL---RYKEATKRRPRS-LLEKLRWVTV 212

Query: 202 GLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGG 261
           G  ++W  + Y     +   PS L  L++++AAA     E+F+ EA I+NY+    TLG 
Sbjct: 213 GYHYNWDSKKYSAD-HYTPFPSDLGFLSEQVAAAC--GFEDFRAEAGILNYYRLDSTLGI 272

Query: 262 HLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFH 318
           H+D  E+D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H
Sbjct: 273 HVDRSELDHSKPLLSFSFGQSAIFLLGGLQRDEAPTAMFMHSGDIMIMSGFSRLLNH 287

[Close]

BLAST of HG10015103 vs. ExPASy Swiss-Prot
Match: P0CB42 (Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.9e-25
Identity = 78/236 (33.05%), Postives = 109/236 (46.19%), Query Frame = 0


Query: 82  LENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNHNAIYGPIKDLFIAAKGKKVLV 141
           LE  PGF FIP       Q  W+++ L  + Q PN                         
Sbjct: 93  LEGYPGFIFIPNPFLPGCQRHWVKQCLKLYSQKPNVC----------------------- 152

Query: 142 EDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRGSACKSIPASVLLRKLRWSTLG 201
                   N+D  +    + G    W   E+     R     K  P S LL +LRW TLG
Sbjct: 153 --------NLDKHMTKEETQGL---W---EQSKEVLRSKEVTKRRPRS-LLERLRWVTLG 212

Query: 202 LQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGH 261
             ++W  + Y     +   PS L  L++++A A    G  F+ EA I+NY+    TLG H
Sbjct: 213 YHYNWDSKKYSAD-HYTPFPSDLAFLSEQVATACGFQG--FQAEAGILNYYRLDSTLGIH 272

Query: 262 LDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFH 318
           +D  E+D SKP++S S G  AIFLLGG  R + P AMF+ SGD+++M+G +R   H
Sbjct: 273 VDRSELDHSKPLLSFSFGQSAIFLLGGLKRDEAPTAMFMHSGDIMVMSGFSRLLNH 287

[Close]

BLAST of HG10015103 vs. ExPASy Swiss-Prot
Match: O60066 (Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=abh1 PE=2 SV=3)

HSP 1 Score: 111.7 bits (278), Expect = 1.7e-23
Identity = 84/307 (27.36%), Postives = 137/307 (44.63%), Query Frame = 0


Query: 12  ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQDGALPLGVYAT 71
           +   FR  EK+YK   D          +P   D+SEV+D          +     G +  
Sbjct: 8   QANVFRLEEKRYKCRAD---------TIP---DMSEVLD---------PNDPQSFG-FEA 67

Query: 72  KCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL-TNFPQPPNRTNHNAIYGPIKDL 131
             ++   VF  +  PG   +   +S + Q Q ++  + T    P N+TN +  Y      
Sbjct: 68  LVEIKPRVFSFQKAPGLLILKNYVSSELQMQLLKSIMFTQIQDPENKTNLSPFY------ 127

Query: 132 FIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRGSACKSIPASV 191
                  ++ + +D I     + D E S+ +G         +  V               
Sbjct: 128 -------QLPLGNDSIWRRYYNGDGE-SIIDGLGETKPLTVDRLVH-------------- 187

Query: 192 LLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVN 251
             +KLRW TLG Q+DW+ + Y         P  L    +++   +      +K EAAIVN
Sbjct: 188 --KKLRWVTLGEQYDWTTKEYPDPSKSPGFPKDLGDFVEKVVKESTDF-LHWKAEAAIVN 247

Query: 252 YFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAG 311
           +++ GDTL  H+D+ E D + P++S+S+G   I+L+G +SR + P A+ L SGDVV+M G
Sbjct: 248 FYSPGDTLSAHIDESEEDLTLPLISLSMGLDCIYLIGTESRSEKPSALRLHSGDVVIMTG 261

Query: 312 EARECFH 318
            +R+ FH
Sbjct: 308 TSRKAFH 261

[Close]

BLAST of HG10015103 vs. ExPASy TrEMBL
Match: A0A1S3BE32 (LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis melo OX=3656 GN=LOC103488884 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.4e-171
Identity = 294/318 (92.45%), Postives = 305/318 (95.91%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE YKQ
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYKQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLD PVFCLENRPGFYFIPGALSLQEQCQWIRESL +FPQPPNRTNH
Sbjct: 61  DGSLPVGVKATTCDLDRPVFCLENRPGFYFIPGALSLQEQCQWIRESLMDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK KKVLVE DEISDFN+DSDVEPS+SNGSTHNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKKVLVEHDEISDFNLDSDVEPSISNGSTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +ACKSI ASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TACKSISASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKA FLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAXFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]

BLAST of HG10015103 vs. ExPASy TrEMBL
Match: A0A0A0KS00 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G589370 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.2e-170
Identity = 292/318 (91.82%), Postives = 304/318 (95.60%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPK VDLSEVIDFK ILE Y+Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKHVDLSEVIDFKNILESYQQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG+LP+GV AT CDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESL  FPQPPNRTNH
Sbjct: 61  DGSLPVGVNATTCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLMEFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK  KVLVE DEISDF +DSDVEPS+SNG+THNWKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKENKVLVEHDEISDFKLDSDVEPSISNGNTHNWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           +A KSIPASVLLRKLRWSTLGLQFDWSKRSY+ISLPHNKIPSALCQLAKRMAAAAMPTGE
Sbjct: 181 TAYKSIPASVLLRKLRWSTLGLQFDWSKRSYNISLPHNKIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPP+AMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPIAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 318

[Close]

BLAST of HG10015103 vs. ExPASy TrEMBL
Match: A0A6J1FZQ9 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=LOC111449397 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.8e-167
Identity = 292/336 (86.90%), Postives = 311/336 (92.56%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DD+ERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFK ILECY Q
Sbjct: 1   MYGSDKGTDDAERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLGV ATKCDLDGPVFCLENRPGFYFIPGALSL+EQCQWIRESL +FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK K+VLVED+EIS  NVDSD EPS+ NG+++ WKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSLINGNSNGWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           + CKS+PAS LLRKLRWSTLGLQFDWSKRSYDISLPHN+IPSALCQL KRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNEIPSALCQLTKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQ 337
           RSGDVVLMAGEARECFHG V  +FT    + + L +
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFTDEESEEISLLE 334

[Close]

BLAST of HG10015103 vs. ExPASy TrEMBL
Match: A0A6J1HQB4 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LOC111466401 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.7e-165
Identity = 293/336 (87.20%), Postives = 309/336 (91.96%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFK ILECY Q
Sbjct: 1   MYGSDKGTDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKRILECYNQ 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLGV ATKCDLDGPVFCLENRPGFYFIPGALSL+EQCQ IRESL +FPQPPNRTNH
Sbjct: 61  DGALPLGVNATKCDLDGPVFCLENRPGFYFIPGALSLEEQCQRIRESLKDFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK K+VLVED+EIS  NVDSD EPSV NG+++ WKFVEE+TVSSRRG
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEDNEISASNVDSDTEPSVINGNSNGWKFVEENTVSSRRG 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           + CKS+PAS LLRKLRWSTLGLQFDWSKRSYDISLPHN IPSALCQLAKRMAAAAMPTGE
Sbjct: 181 T-CKSVPASALLRKLRWSTLGLQFDWSKRSYDISLPHNDIPSALCQLAKRMAAAAMPTGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQD PVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDLPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHGNVALMFTLTSYQNVFLYQ 337
           RSGDVVLMAGEARECFHG V  +FT    + + L +
Sbjct: 301 RSGDVVLMAGEARECFHG-VPRIFTDEESEEISLLE 334

[Close]

BLAST of HG10015103 vs. ExPASy TrEMBL
Match: A0A6J1DG90 (alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN=LOC111019811 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.3e-164
Identity = 284/318 (89.31%), Postives = 300/318 (94.34%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MYGSD+G DD ERTAFR AEKKYK YYDDT+KSSKKKKLPKQVDLSEVIDFK ILECY  
Sbjct: 1   MYGSDKGTDDLERTAFRIAEKKYKFYYDDTFKSSKKKKLPKQVDLSEVIDFKRILECYNH 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DGALPLG+ AT+CDLDGPVFCLENRPGFYFIPGALSL+EQCQWIRESLT+FPQPPNRTNH
Sbjct: 61  DGALPLGMNATQCDLDGPVFCLENRPGFYFIPGALSLEEQCQWIRESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI+DLFIAAK K+VLVE +EIS FNVDSD+EPSVS G+TH WKFVEE+TVSSRR 
Sbjct: 121 NAIYGPIQDLFIAAKEKRVLVEANEISAFNVDSDIEPSVSTGNTHGWKFVEENTVSSRR- 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           + CKS+PASVLLRKLRWSTLGLQFDWSKRSYDISL HNK+PSALCQLAKRMAAAAMP GE
Sbjct: 181 TTCKSVPASVLLRKLRWSTLGLQFDWSKRSYDISLLHNKMPSALCQLAKRMAAAAMPAGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EFKPEAAIVNYFASGD+LGGHLDDME DWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL
Sbjct: 241 EFKPEAAIVNYFASGDSLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 317

[Close]

BLAST of HG10015103 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 451.1 bits (1159), Expect = 8.4e-127
Identity = 219/318 (68.87%), Postives = 248/318 (77.99%), Query Frame = 0


Query: 1   MYGSDQGIDDSERTAFRRAEKKYKLYYDDTYKSSKKKKLPKQVDLSEVIDFKCILECYKQ 60
           MY S    DD++RTAFRRAEKKYKLYY+   K S+KKKLPK +DLSE++DF  I + +  
Sbjct: 1   MYESANVSDDADRTAFRRAEKKYKLYYEQDSKFSRKKKLPKPIDLSELLDFNLISQNFNN 60

Query: 61  DGALPLGVYATKCDLDGPVFCLENRPGFYFIPGALSLQEQCQWIRESLTNFPQPPNRTNH 120
           DG LP G+  +K D   PVFC++NRPGFYFIP ALSL+EQC+WI+ESLT+FPQPPNRTNH
Sbjct: 61  DGVLPDGIRVSKVD-SSPVFCIDNRPGFYFIPDALSLKEQCKWIKESLTSFPQPPNRTNH 120

Query: 121 NAIYGPIKDLFIAAKGKKVLVEDDEISDFNVDSDVEPSVSNGSTHNWKFVEEHTVSSRRG 180
           NAIYGPI DLF +AK  KVLV+DD                  + + WKF EE  +     
Sbjct: 121 NAIYGPIDDLFDSAKENKVLVQDDL-----------------TNNKWKFYEEVDIEKATR 180

Query: 181 SACKSIPASVLLRKLRWSTLGLQFDWSKRSYDISLPHNKIPSALCQLAKRMAAAAMPTGE 240
           S+CKS+ ASVLLRKLRWSTLGLQFDWSKR+YD+SLPHN IP ALCQLAK  AA AMP GE
Sbjct: 181 SSCKSVSASVLLRKLRWSTLGLQFDWSKRNYDVSLPHNNIPDALCQLAKTHAAIAMPDGE 240

Query: 241 EFKPEAAIVNYFASGDTLGGHLDDMEVDWSKPIVSMSLGCKAIFLLGGKSRQDPPVAMFL 300
           EF+PE AIVNYF  GDTLGGHLDDME DWSKPIVSMSLGCKAIFLLGGKS+ DPP AM+L
Sbjct: 241 EFRPEGAIVNYFGIGDTLGGHLDDMEADWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYL 300

Query: 301 RSGDVVLMAGEARECFHG 319
           RSGDVVLMAGEARECFHG
Sbjct: 301 RSGDVVLMAGEARECFHG 300

[Close]

BLAST of HG10015103 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 2.5e-09
Identity = 32/79 (40.51%), Postives = 45/79 (56.96%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEVDWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++S   LG H D  E + S     P+VS S+G  A FL G +  +D    + 
Sbjct: 346 PDICIVNFYSSTGRLGLHQDKDESENSIRKGLPVVSFSIGDSAEFLYGDQRDEDKAETLT 405

Query: 304 LRSGDVVLMAGEARECFHG 319
           L SGDV+L  G +R+ FHG
Sbjct: 406 LESGDVLLFGGRSRKVFHG 424

[Close]

BLAST of HG10015103 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-08
Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEVDWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++    LG H D  E + S     PIVS S+G  A FL G K   +    + 
Sbjct: 278 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 337

Query: 304 LRSGDVVLMAGEARECFHG 319
           L SGDV++  GE+R  FHG
Sbjct: 338 LESGDVLIFGGESRMIFHG 356

[Close]

BLAST of HG10015103 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-08
Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 244 PEAAIVNYFASGDTLGGHLDDMEVDWS----KPIVSMSLGCKAIFLLGGKSRQDPPVAMF 303
           P+  IVN+++    LG H D  E + S     PIVS S+G  A FL G K   +    + 
Sbjct: 333 PDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVI 392

Query: 304 LRSGDVVLMAGEARECFHG 319
           L SGDV++  GE+R  FHG
Sbjct: 393 LESGDVLIFGGESRMIFHG 411

[Close]

BLAST of HG10015103 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 43.1 bits (100), Expect = 5.3e-04
Identity = 20/47 (42.55%), Postives = 26/47 (55.32%), Query Frame = 0


Query: 272 PIVSMSLGCKAIFLLGGKSRQDPPVAMFLRSGDVVLMAGEARECFHG 319
           PIVS S+G  A FL G +   D    + L SGDV++    +R  FHG
Sbjct: 404 PIVSFSIGDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHG 450

The following BLAST results are available for this feature:

BLAST of HG10015103 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891364.1	1.7e-174	94.65	alpha-ketoglutarate-dependent dioxygenase alkB [Benincasa hispida]	[more]
XP_008446031.1	1.5e-170	92.45	PREDICTED: LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB [...	[more]
XP_004135327.1	1.7e-169	91.82	alpha-ketoglutarate-dependent dioxygenase alkB isoform X1 [Cucumis sativus]	[more]
XP_022945036.1	7.8e-167	86.90	alpha-ketoglutarate-dependent dioxygenase alkB [Cucurbita moschata]	[more]
KAG6573589.1	1.3e-166	86.90	Alpha-ketoglutarate-dependent dioxygenase alkB, partial [Cucurbita argyrosperma ...	[more]

BLAST of HG10015103 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SA98	1.2e-125	68.87	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Arabidopsis thaliana OX=3702 G...	[more]
Q54N08	3.2e-38	31.04	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44...	[more]
Q13686	2.2e-26	33.33	Nucleic acid dioxygenase ALKBH1 OS=Homo sapiens OX=9606 GN=ALKBH1 PE=1 SV=2	[more]
P0CB42	6.9e-25	33.05	Nucleic acid dioxygenase ALKBH1 OS=Mus musculus OX=10090 GN=Alkbh1 PE=1 SV=1	[more]
O60066	1.7e-23	27.36	Alpha-ketoglutarate-dependent dioxygenase abh1 OS=Schizosaccharomyces pombe (str...	[more]

BLAST of HG10015103 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BE32	7.4e-171	92.45	LOW QUALITY PROTEIN: alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucumis m...	[more]
A0A0A0KS00	8.2e-170	91.82	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1FZQ9	3.8e-167	86.90	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQB4	2.7e-165	87.20	alpha-ketoglutarate-dependent dioxygenase alkB OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1DG90	2.3e-164	89.31	alpha-ketoglutarate-dependent dioxygenase alkB OS=Momordica charantia OX=3673 GN...	[more]

BLAST of HG10015103 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11780.1	8.4e-127	68.87	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT3G14160.1	2.5e-09	40.51	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.1	1.6e-08	40.51	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.2	1.6e-08	40.51	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT3G14140.1	5.3e-04	42.55	2-oxoglutarate-dependent dioxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 164..325 e-value: 1.1E-40 score: 141.5
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 188..318 e-value: 5.0E-34 score: 118.2
None	No IPR available	PANTHER	PTHR16557:SF8	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB	coord: 1..325
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 86..318
IPR004574	Alkylated DNA repair protein AlkB	PANTHER	PTHR16557	ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED	coord: 1..325

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10015103.1	HG10015103.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
biological_process	GO:0035513	oxidative RNA demethylation
biological_process	GO:0035552	oxidative single-stranded DNA demethylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0035516	oxidative DNA demethylase activity
molecular_function	GO:0035515	oxidative RNA demethylase activity