HG10015097 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10015097
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description4-coumarate--CoA ligase-like 1
LocationChr02: 23843147 .. 23845422 (+)
RNA-Seq ExpressionHG10015097
SyntenyHG10015097
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACTTGCATTCAAGATTTGGTAGAAGATGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGGTGATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCGTTTGTGGAAGCCGTGAGCGGAAAGGCATACACTTACCGCGAAGTCGTGAGAGACACAAATAGATTTGCTAAGGCCTTGTGCTCTCTGAGGTTGAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTTGCAGACGCCAAACTCGTCGTCACAAACAGTGCAAACTTTGAAAAGGTAAGATAGAAACTGTTGTTGATATTCATCGTATTTCTTTCGAATACAATTCGTCGCACTTACTATCTATGTTGTTGAGAACAGGTGAAGGAATTAAAGCTGCCAGTGATCTTATTGGGGGATGAACTAATTGAAGGTTCCATGAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACAATTTTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGAACCACTGGGGTTTCCAAAGGTGTTATGCTAACTCACCGAAATCTAGTAGCTAATTTGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGTCTCATTCCGTTCTTCCATATTTATGGGATCACTGGAATATGTTGTGCCACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCTCTCATAACACAGGAAGTCACATTTGCTCCAATTGTTCCTCCTATCATCCTGGCTTTGGTTAAGAATCCTATTGTGGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAGCTTCAAACTGCTTTCGAAAGAAAGTTCCCGGGCGTGGATGTTCAAGAGGTAATTTAGTTATTACTGTTGAGTGTAGTAGATAGATTGTGACAATCTCCACTCTCGTTCATTCCTTCAAATTTTTTAGCTATAACATGTAACCCAACTTCCTGTTTTCGGCACATCAGAAGTGAATCGAGCTCCACCGCGACATTGCGGTCCATGGGATCCTAATTTGTATAATATGCTAACTAATTGTCGTTGATCACGGACAGGCATATGGACTAACCGAGCACAGCTGCATCACTCTCAACTATGGAAGTATAGGCAAAGAGAATCTCACTGCAAAGAAAAAAACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACAGTGGAAGGTCTCTGCCGAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAGGGTACGGAACATAGTTCGATGCAGAAAGTCTTTATGAGATCTCTAATTAAAAACCAGCTGAATCTTTCCTTCTTGTTTCAGGCTACTACAAAAATGAAGAAGAGACTTCAAGGACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAATACAAAGGCTTTCAAGTAAAGACATAACATAACTTATTTCAAGTTGATCCATTGATCGATAAAAAGGAAGCTGAAGATGTGAAAAATGGAAGTTTGCATATCAAACTTTTTTTGTACATATGAATATGAAATAAGGTGGTGGCTGTCTTTGATTTCTTTGCAGGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTACTCATCCCTCCATTGAAGACGCAGCTGTCGTGCCGTAAGATTCTTCGCCTCACATTCATTTTGCTTCATGTTGTTTCAATTCAAAGATAAATCAGATTAAAACAATGCTATGCTTGCATCGGCGTTTCAGTCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTCGTGATGGCTCCAAATTCAAAAGAAACTGAAGAGGAAATAATCAAACACGTTGCCTCAAACGTCGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGAATCCGCCCTTAGATCTGTCTGA

mRNA sequence

ATGGCTACTTGCATTCAAGATTTGGTAGAAGATGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGGTGATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCGTTTGTGGAAGCCGTGAGCGGAAAGGCATACACTTACCGCGAAGTCGTGAGAGACACAAATAGATTTGCTAAGGCCTTGTGCTCTCTGAGGTTGAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTTGCAGACGCCAAACTCGTCGTCACAAACAGTGCAAACTTTGAAAAGGTGAAGGAATTAAAGCTGCCAGTGATCTTATTGGGGGATGAACTAATTGAAGGTTCCATGAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACAATTTTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGAACCACTGGGGTTTCCAAAGGTGTTATGCTAACTCACCGAAATCTAGTAGCTAATTTGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGTCTCATTCCGTTCTTCCATATTTATGGGATCACTGGAATATGTTGTGCCACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCTCTCATAACACAGGAAGTCACATTTGCTCCAATTGTTCCTCCTATCATCCTGGCTTTGGTTAAGAATCCTATTGTGGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAGCTTCAAACTGCTTTCGAAAGAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGACTAACCGAGCACAGCTGCATCACTCTCAACTATGGAAGTATAGGCAAAGAGAATCTCACTGCAAAGAAAAAAACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACAGTGGAAGGTCTCTGCCGAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAGGGCTACTACAAAAATGAAGAAGAGACTTCAAGGACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAATACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTACTCATCCCTCCATTGAAGACGCAGCTGTCGTGCCTCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTCGTGATGGCTCCAAATTCAAAAGAAACTGAAGAGGAAATAATCAAACACGTTGCCTCAAACGTCGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGAATCCGCCCTTAGATCTGTCTGA

Coding sequence (CDS)

ATGGCTACTTGCATTCAAGATTTGGTAGAAGATGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGGTGATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCGTTTGTGGAAGCCGTGAGCGGAAAGGCATACACTTACCGCGAAGTCGTGAGAGACACAAATAGATTTGCTAAGGCCTTGTGCTCTCTGAGGTTGAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTTGCAGACGCCAAACTCGTCGTCACAAACAGTGCAAACTTTGAAAAGGTGAAGGAATTAAAGCTGCCAGTGATCTTATTGGGGGATGAACTAATTGAAGGTTCCATGAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACAATTTTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGAACCACTGGGGTTTCCAAAGGTGTTATGCTAACTCACCGAAATCTAGTAGCTAATTTGTGTTCGACTCTCTCTGGTGTCCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGTCTCATTCCGTTCTTCCATATTTATGGGATCACTGGAATATGTTGTGCCACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCTCTCATAACACAGGAAGTCACATTTGCTCCAATTGTTCCTCCTATCATCCTGGCTTTGGTTAAGAATCCTATTGTGGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAGCTTCAAACTGCTTTCGAAAGAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGACTAACCGAGCACAGCTGCATCACTCTCAACTATGGAAGTATAGGCAAAGAGAATCTCACTGCAAAGAAAAAAACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCAGACAGTGGAAGGTCTCTGCCGAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAGGGCTACTACAAAAATGAAGAAGAGACTTCAAGGACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAATACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTACTCATCCCTCCATTGAAGACGCAGCTGTCGTGCCTCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGCGTCGTGATGGCTCCAAATTCAAAAGAAACTGAAGAGGAAATAATCAAACACGTTGCCTCAAACGTCGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGAATCCGCCCTTAGATCTGTCTGA

Protein sequence

MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRAESALRSV
Homology
BLAST of HG10015097 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 531/549 (96.72%), Postives = 540/549 (98.36%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATC +DLVEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDTNRF+KAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVA+AKLV+TNSANFEKVKELKLPVILLG+ELIEGSMNWHKLLEAADRAGNN VKE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPQEM GKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LSGLKLQAIMTAAAPLAPELQ+AFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENL AK
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLAAK 360

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAASVVMAPNSKETEEEIIKHVASNVA+YKKVRLVHFVD IPKSPSGKVMRRL+KEKM
Sbjct: 481 EIPAASVVMAPNSKETEEEIIKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKM 540

Query: 541 IEKIRAESA 550
           IEKIRAESA
Sbjct: 541 IEKIRAESA 549

BLAST of HG10015097 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.1e-297
Identity = 524/549 (95.45%), Postives = 544/549 (99.09%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+DLVEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYA+NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWHKLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LSGLKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VMAPN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAEAA 605

BLAST of HG10015097 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.2e-296
Identity = 522/549 (95.08%), Postives = 542/549 (98.72%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+D VEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYA+NVAFVEAVSG AYTYR
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWHKLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LSGLKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VMAPN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of HG10015097 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1027.3 bits (2655), Expect = 4.8e-296
Identity = 521/549 (94.90%), Postives = 542/549 (98.72%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+D VEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYA+NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWH+LLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LSGLKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VM PN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of HG10015097 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.1e-295
Identity = 518/549 (94.35%), Postives = 540/549 (98.36%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+D  EDEEHIFRSQLPEVQVP DITLPEFVLQNAE YA+NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWHKLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LS LKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYG+IGK+NL AK
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VMAPN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of HG10015097 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 8.4e-227
Identity = 392/532 (73.68%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 69
           ED E+IFRS  P V +P  +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 129
           AKAL SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 189
           + ++T++ N+EKVK L LPVI+LG+E IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 309
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRILP 369
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    AK+ +VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 429
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 489
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 APNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 542
            P + E EE+I+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of HG10015097 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 703.4 bits (1814), Expect = 2.1e-201
Identity = 350/539 (64.94%), Postives = 438/539 (81.26%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVS-GKAYTYREVVRDTNR 69
           E++EH+FRS+ P V VP  +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 70  FAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 129
           FA+AL S+ ++KG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 130 AKLVVTNSANFEKVKELKLPVILLGD-ELIEGSMNWHKLLEAADRAGNNFVKED-IKQTD 189
           AKLVV N   F+KVK+  +PVI +GD E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 190 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGI 249
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 250 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 309
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 310 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGR 369
           +++MTAAAPLAP+L  AF+RKFPGV V+EAYGLTEHSCITL + +       AKK +VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 370 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 429
           ILPNLEVKF+DPD+GRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 430 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 489
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 490 VVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 546
           VV    ++E EEEI+ +VA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of HG10015097 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.8e-113
Identity = 213/534 (39.89%), Postives = 328/534 (61.42%), Query Frame = 0

Query: 12  EEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 71
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 72  ALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 131
            L  L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 132 VVTNSANFEKVKEL---KLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLC 191
           ++T +   +KVK+    K   I+  D+  +  +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 192 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 251
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 252 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 311
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 312 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGR 371
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 372 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 431
           ++ N E+K +DP++  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 432 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 491
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 492 VVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
           VV       TEEEI + V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of HG10015097 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 8.1e-113
Identity = 214/534 (40.07%), Postives = 328/534 (61.42%), Query Frame = 0

Query: 12  EEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAK 71
           E+ IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 72  ALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 131
            L  L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 128

Query: 132 VVTNSANFEKVKEL---KLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLC 191
           ++T +   +KVK+    K   I+  D+  +  +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---IDSDDVV 188

Query: 192 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 251
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 252 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 311
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 312 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGR 371
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 372 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIG 431
           ++ N E+K +DP++  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 432 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 491
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 492 VVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
           VV       TEEEI + V+  V  YK++  V FVD IPKSPSGK++R+ ++ K+
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKI 537

BLAST of HG10015097 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.8e-112
Identity = 204/531 (38.42%), Postives = 327/531 (61.58%), Query Frame = 0

Query: 15  IFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALC 74
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + A  L 
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 75  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 134
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 135 NSANFEKVKELKLP---VILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALP 194
            +    KVK+  +     ++  D + EG +++ +L+++ +   +      I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDE---HEIPDVKIQPDDVVALP 193

Query: 195 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGICCA 254
           +SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 255 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 314
            LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+ +DLS ++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--TV 313

Query: 315 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRILP 374
           M+ AAPL  EL+ A   KFP   + + YG+TE   +     +  KE    K    G ++ 
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVR 373

Query: 375 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 434
           N E+K +DPD+G SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+HTGDIG+ID
Sbjct: 374 NAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFID 433

Query: 435 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 494
           DD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV 
Sbjct: 434 DDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVR 493

Query: 495 APNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
           +  S  TE+E+   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 494 SNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of HG10015097 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1032.7 bits (2669), Expect = 5.5e-298
Identity = 524/549 (95.45%), Postives = 544/549 (99.09%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+DLVEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYA+NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWHKLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LSGLKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AK
Sbjct: 357 LSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VMAPN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAEAA 605

BLAST of HG10015097 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.0e-295
Identity = 518/549 (94.35%), Postives = 540/549 (98.36%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATCI+D  EDEEHIFRSQLPEVQVP DITLPEFVLQNAE YA+NVAFVEAVSGKAYTYR
Sbjct: 57  MATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYR 116

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT+RFAKAL SLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 117 EVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 176

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVADAKLVVTNS++FEKVKELKLPVI++G+ELIEGSMNWHKLLEAADRAGNNFVKE
Sbjct: 177 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE 236

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEMVGKVTTLGLIPFFHI
Sbjct: 237 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHI 296

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFD
Sbjct: 297 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFD 356

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LS LKLQAIMTAAAPLAPELQT+FE+KFPGVDVQEAYGLTEHSCITLNYG+IGK+NL AK
Sbjct: 357 LSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAK 416

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDNKGWM
Sbjct: 417 KNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWM 476

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAG
Sbjct: 477 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAG 536

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAAS+VMAPN+KETE+EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM
Sbjct: 537 EIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 596

Query: 541 IEKIRAESA 550
           IEKIRAE+A
Sbjct: 597 IEKIRAETA 605

BLAST of HG10015097 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.6e-292
Identity = 516/553 (93.31%), Postives = 537/553 (97.11%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           MATC QD V+DEEHIF SQLPEVQVP DITLPEFVLQNAESYAENVAFVEA+SGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EV+RDTNRF+KAL SLRLKKG VVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVEVA+AKLVVTN+ANFEKV+ELKLPVILL +EL+EG+MNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           +IKQTDLCALPFSSGTTGVSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICC+TLRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LS LKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEH CITLNYGSIGKENL+AK
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDPD+GRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAASVVMAPNSKETEE+IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 541 IEKIRAESALRSV 554
           IEKIRA+ + R +
Sbjct: 541 IEKIRADESARFI 553

BLAST of HG10015097 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.7e-291
Identity = 515/549 (93.81%), Postives = 536/549 (97.63%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           M T I+DLVEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 60  MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 119

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT RFAKAL SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 120 EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 179

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS NFEKVKELKLPVI+LG+ELIEGSMNWHKLLEAADRAGNN VKE
Sbjct: 180 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 239

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIPFFHI
Sbjct: 240 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 299

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 300 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 359

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LS LKLQAIMTAAAPLAPE+QTAFE+KFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK
Sbjct: 360 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 419

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDP+SGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 420 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 479

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 480 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 539

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+APNSKETEEEII++VASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIKEKM
Sbjct: 540 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 599

Query: 541 IEKIRAESA 550
           I+KIRA++A
Sbjct: 600 IDKIRAKTA 608

BLAST of HG10015097 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.7e-291
Identity = 515/549 (93.81%), Postives = 536/549 (97.63%), Query Frame = 0

Query: 1   MATCIQDLVEDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60
           M T I+DLVEDEEHIFRSQLPEVQVP DITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 9   MGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 68

Query: 61  EVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120
           EVVRDT RFAKAL SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 69  EVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 128

Query: 121 KKQVEVADAKLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE 180
           KKQVE ADAKLVVTNS NFEKVKELKLPVI+LG+ELIEGSMNWHKLLEAADRAGNN VKE
Sbjct: 129 KKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKE 188

Query: 181 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHI 240
           DIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIPFFHI
Sbjct: 189 DIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHI 248

Query: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300
           YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 249 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 308

Query: 301 LSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 360
           LS LKLQAIMTAAAPLAPE+QTAFE+KFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK
Sbjct: 309 LSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAK 368

Query: 361 KKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWM 420
           K TVGRILPNLEVKFIDP+SGRSLPKNTPGEICVRSQCVMQGYY NEEETSRTIDN+GW+
Sbjct: 369 KNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWL 428

Query: 421 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480
           HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 429 HTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 488

Query: 481 EIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540
           EIPAASVV+APNSKETEEEII++VASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIKEKM
Sbjct: 489 EIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKM 548

Query: 541 IEKIRAESA 550
           I+KIRA++A
Sbjct: 549 IDKIRAKTA 557

BLAST of HG10015097 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 787.7 bits (2033), Expect = 6.0e-228
Identity = 392/532 (73.68%), Postives = 454/532 (85.34%), Query Frame = 0

Query: 10  EDEEHIFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRF 69
           ED E+IFRS  P V +P  +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 70  AKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 129
           AKAL SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 130 KLVVTNSANFEKVKELKLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 189
           + ++T++ N+EKVK L LPVI+LG+E IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 190 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 249
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 250 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 309
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 310 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRILP 369
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEHSCITL +G   K    AK+ +VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 370 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 429
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 430 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 489
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 490 APNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 542
            P + E EE+I+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of HG10015097 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 399.1 bits (1024), Expect = 6.0e-111
Identity = 205/532 (38.53%), Postives = 326/532 (61.28%), Query Frame = 0

Query: 15  IFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALC 74
           IFRS+LP++ +P  + L +++ +N   +A     +   +G+ YTY +V   + + A  L 
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 75  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 134
           +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 135 NSANFEKVKELKLPVILL----GDELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCAL 194
            S   +K+K L+   +L+     D + E  + + +L ++ +   ++ + E I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 195 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGITGICC 254
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 255 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQA 314
            +LR    +++M +F++   +  +   +VT A +VPPI+LA+ K+P  E++DLS +++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 315 IMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRIL 374
           + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 375 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYI 434
            N E+K +DPD+G SLP+N PGEIC+R   +M+GY  +   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 435 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVV 494
           DDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A VV
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 495 MAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
            + +S  +E+EI + V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of HG10015097 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 387.1 bits (993), Expect = 2.4e-107
Identity = 200/536 (37.31%), Postives = 319/536 (59.51%), Query Frame = 0

Query: 15  IFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALC 74
           IFRS+LP++ +P  ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 75  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 134
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 135 NSANFEKVKELK----LPVILLGDE----LIEGSMNWHKLLEAADRAGNNFVKEDIKQTD 194
            +   +K+K L+    + ++ + D     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 195 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGIT 254
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 255 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGL 314
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   E++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 315 KLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTV 374
           ++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 375 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGD 434
           G ++ N E+K +DPD+G SL +N PGEIC+R   +M+GY  N   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 435 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPA 494
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + +E AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 495 ASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
           A VV + +S+ +E+++ + V+  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of HG10015097 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 380.2 bits (975), Expect = 2.9e-105
Identity = 206/531 (38.79%), Postives = 320/531 (60.26%), Query Frame = 0

Query: 15  IFRSQLPEVQVPGDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTNRFAKALC 74
           IFRS+LP++ +P  + L  +  +   S ++    +   +GK+YTY E      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 75  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 134
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 135 NSANFEKVKEL--KLPVILLGDELIEGSMNWHKLLEAADRAGNNFVKE-DIKQTDLCALP 194
           +S   +K+K L   L +I   +   E  + +  L+   D   N F +  DI   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 195 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKV--TTLGLIPFFHIYGITGICCA 254
           FSSGTTG+ KGV+LTH++L+ ++   + G    +  K     L ++P FHIY +  +   
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 255 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 314
           +LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS ++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 315 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKKTVGRILP 374
           ++ AAPL  ELQ +  R+ P   + + YG+TE   +        KE +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 375 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDNKGWMHTGDIGYID 434
           N E+K +  ++  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 435 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 494
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 495 APNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 541
           +  +  TEE++ ++VA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of HG10015097 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 377.5 bits (968), Expect = 1.9e-104
Identity = 199/554 (35.92%), Postives = 329/554 (59.39%), Query Frame = 0

Query: 6   QDLVEDEEH--IFRSQLPEVQVPGDITLPEFVLQ----NAESYAENVAFVEAVSGKAYTY 65
           +D  E+  H  IFRS+LP++ +P  + L ++V Q    + +  +     ++  +G+  TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 66  REVVRDTNRFAKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISE 125
            +V  +  R A  +  L ++ G VV+++LPN  E+A+  L +   G V +  NP     E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 126 IKKQVEVADAKLVVTNSANFEKVKELKLPVILL------GDELI-----EGSMNWHKLLE 185
           I KQ + + AK+++T     +K+  LK   +L+      GD  +     +G +++ +L +
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 196

Query: 186 AADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--V 245
           A +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +   
Sbjct: 197 ADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFT 256

Query: 246 GKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPI 305
                L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP+
Sbjct: 257 ANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPV 316

Query: 306 ILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHSCIT 365
           +LA +K+P  E +DLS +++  +++ AA L  EL+ A   KFP     + YG+TE   + 
Sbjct: 317 VLAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVA 376

Query: 366 LNYGSIGKENLTAKKKTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYKN 425
            +  +  K     K    G ++ N E+K +D ++G SLP+N  GEICVR   +M+GY  +
Sbjct: 377 KSL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLND 436

Query: 426 EEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSI 485
            E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI
Sbjct: 437 PEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSI 496

Query: 486 EDAAVVPLPDEEAGEIPAASVVMAPNSKETEEEIIKHVASNVAHYKKVRLVHFVDTIPKS 541
           +DAAVV + DE A E+P A V  +  S+ TE+++  +V   V HYK++++V F++ IPK+
Sbjct: 497 DDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKA 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893074.11.6e-29996.724-coumarate--CoA ligase-like 1 [Benincasa hispida][more]
XP_022945246.11.1e-29795.454-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
XP_023541569.12.2e-29695.084-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo][more]
KAG7012722.14.8e-29694.904-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022966799.14.1e-29594.354-coumarate--CoA ligase-like 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LQ128.4e-22773.684-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL22.1e-20164.944-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P149124.8e-11339.894-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
P149138.1e-11340.074-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
P316841.8e-11238.424-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0B45.5e-29895.454-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV52.0e-29594.354-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A0A0KRZ51.6e-29293.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1[more]
A0A6J1DG051.7e-29193.814-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DEE11.7e-29193.814-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G62940.16.0e-22873.68acyl-CoA synthetase 5 [more]
AT3G21240.16.0e-11138.534-coumarate:CoA ligase 2 [more]
AT1G51680.12.4e-10737.314-coumarate:CoA ligase 1 [more]
AT1G65060.12.9e-10538.794-coumarate:CoA ligase 3 [more]
AT3G21230.11.9e-10435.924-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 455..530
e-value: 4.7E-18
score: 65.9
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 37..446
e-value: 9.2E-96
score: 321.0
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 13..441
e-value: 3.5E-130
score: 436.5
NoneNo IPR availableGENE3D3.30.300.30coord: 442..544
e-value: 9.6E-32
score: 111.7
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 8..543
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 8..543
NoneNo IPR availableCDDcd059044CLcoord: 25..534
e-value: 0.0
score: 665.86
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 20..543
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 190..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10015097.1HG10015097.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity