HG10015048 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015048 vs. NCBI nr
Match: XP_008445949.1 (PREDICTED: WAT1-related protein At5g07050 [Cucumis melo])

HSP 1 Score: 647.1 bits (1668), Expect = 9.2e-182
Identity = 349/388 (89.95%), Postives = 357/388 (92.01%), Query Frame = 0

Query: 1   MGKL--SLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60
           MGKL    NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT
Sbjct: 1   MGKLISFFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60

Query: 61  ASIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120
           ASIAPFVLFFERRGQPR+T KVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML
Sbjct: 61  ASIAPFVLFFERRGQPRMTFKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120

Query: 121 PAMT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTN 180
           PAMT        MEKLEMKKLRCQAKV+GTLV+V GAMLMTLYKGP LQM WSKNSHLTN
Sbjct: 121 PAMTFVMAVIFRMEKLEMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWSKNSHLTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
           S+ESSNNKDWFKGSIFLIIATLAWASLFVL  Q LKTYKNHQFTLT LVCF+GTLQAIAV
Sbjct: 181 SSESSNNKDWFKGSIFLIIATLAWASLFVLQNQVLKTYKNHQFTLTTLVCFMGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV
Sbjct: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKT-SAADDEIPEAIKAS-- 360
           AIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKHKENLE K+ S  D+EIPE IKAS  
Sbjct: 301 AIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSGSDHDNEIPEVIKASNN 360

Query: 361 SQPNI-NNNSIFISMPAPEDPIKPNQIP 373
           SQPNI NNNSIFISMP PE P KPNQIP
Sbjct: 361 SQPNISNNNSIFISMPTPETPTKPNQIP 388

BLAST of HG10015048 vs. NCBI nr
Match: XP_038892785.1 (WAT1-related protein At5g07050 [Benincasa hispida])

HSP 1 Score: 646.7 bits (1667), Expect = 1.2e-181
Identity = 348/382 (91.10%), Postives = 353/382 (92.41%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSLNCCN FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLNCCNSFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERR QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERREQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNS-HLTNS 180
           MT        MEKLEMKKLRCQAKV+GTLVSVAGAMLMTLYKG LLQM WSK+S HLTNS
Sbjct: 121 MTFVMAVIFRMEKLEMKKLRCQAKVLGTLVSVAGAMLMTLYKGSLLQMPWSKHSHHLTNS 180

Query: 181 NESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAVT 240
           NE+SNNKDWFKGSIFLIIATLAWASLFVL  QALKTYKNHQFTLT LVCFLGTLQAIAVT
Sbjct: 181 NETSNNKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAVT 240

Query: 241 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300
           LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA
Sbjct: 241 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300

Query: 301 IMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADDEIPEAIKASSQPN 360
           IMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKT+  DDEIPEAIK SSQ N
Sbjct: 301 IMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTADDDDEIPEAIKPSSQLN 360

Query: 361 I------NNNSIFISMPAPEDP 366
           I      NNNSIFISMP PE P
Sbjct: 361 INNNNNNNNNSIFISMPTPEKP 382

BLAST of HG10015048 vs. NCBI nr
Match: XP_022138963.1 (WAT1-related protein At5g07050 [Momordica charantia])

HSP 1 Score: 639.4 bits (1648), Expect = 1.9e-179
Identity = 342/402 (85.07%), Postives = 364/402 (90.55%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSL+CCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPFVLFFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFVLFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNS--HLTN 180
           MT        ME L+MKKLRCQAK++GTLV+VAGAMLMTLYKGPL+QMAWSK+S    TN
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSSNSSTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
           S+E+S NKDWFKGSIFLIIATLAWASLFVL  QALKTYKNHQFTLT LVCFLGTLQAIAV
Sbjct: 181 SDENSANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV
Sbjct: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIK--TSAADDEIPEAIKASS 360
           AIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKH+ENLEIK  ++AADDEIPEAIKA+S
Sbjct: 301 AIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHRENLEIKSASAAADDEIPEAIKANS 360

Query: 361 QPNIN--------------NNSIFISMPAPEDPIKPNQIPRE 375
            PN+               +NS+FI++  PE+PIKPNQ+PRE
Sbjct: 361 NPNLQPNNNNNGNMGPNKPSNSLFITISDPENPIKPNQVPRE 402

BLAST of HG10015048 vs. NCBI nr
Match: XP_004151540.1 (WAT1-related protein At5g07050 [Cucumis sativus])

HSP 1 Score: 634.8 bits (1636), Expect = 4.7e-178
Identity = 340/391 (86.96%), Postives = 360/391 (92.07%), Query Frame = 0

Query: 1   MGKLSL-NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATA 60
           MGKLSL NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRH FATA
Sbjct: 1   MGKLSLFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHVFATA 60

Query: 61  SIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLP 120
           SIAPFVLFFERRGQPRIT K+FIQIF+LALLGPV+DQNFYYAGLKYTSPTFSCAMSN+LP
Sbjct: 61  SIAPFVLFFERRGQPRITFKIFIQIFMLALLGPVVDQNFYYAGLKYTSPTFSCAMSNILP 120

Query: 121 AMT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSK-NSHLTN 180
           AMT        MEKL+MKKLRCQAKV+GTLV+V GAMLMTLYKGP LQM W+K NSHLTN
Sbjct: 121 AMTFVMAVIFRMEKLDMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWTKHNSHLTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
           SNESSN+KDWFKGSIFLIIATLAW+SLFVL  QALKTY+NHQFTLT L+CF+GTLQAIAV
Sbjct: 181 SNESSNDKDWFKGSIFLIIATLAWSSLFVLQNQALKTYENHQFTLTTLMCFVGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKK+GPVFATAFSPLMMIIV
Sbjct: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKKGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADD-EIPEAIKAS-- 360
           AIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKHKE+LE K S +DD EIPE IKAS  
Sbjct: 301 AIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKESLENKLSGSDDNEIPEVIKASSN 360

Query: 361 SQPNIN----NNSIFISMPAPEDPIKPNQIP 373
           SQPN N    NN+IFISMP PE+PIKPNQ+P
Sbjct: 361 SQPNTNNNNTNNTIFISMPTPENPIKPNQMP 391

BLAST of HG10015048 vs. NCBI nr
Match: KAE8648642.1 (hypothetical protein Csa_008999 [Cucumis sativus])

HSP 1 Score: 629.0 bits (1621), Expect = 2.6e-176
Identity = 340/406 (83.74%), Postives = 360/406 (88.67%), Query Frame = 0

Query: 1   MGKLSL-NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATA 60
           MGKLSL NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRH FATA
Sbjct: 1   MGKLSLFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHVFATA 60

Query: 61  SIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLP 120
           SIAPFVLFFERRGQPRIT K+FIQIF+LALLGPV+DQNFYYAGLKYTSPTFSCAMSN+LP
Sbjct: 61  SIAPFVLFFERRGQPRITFKIFIQIFMLALLGPVVDQNFYYAGLKYTSPTFSCAMSNILP 120

Query: 121 AMT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSK-NSHLTN 180
           AMT        MEKL+MKKLRCQAKV+GTLV+V GAMLMTLYKGP LQM W+K NSHLTN
Sbjct: 121 AMTFVMAVIFRMEKLDMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWTKHNSHLTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL-----------------QALKTYKNHQFTL 240
           SNESSN+KDWFKGSIFLIIATLAW+SLFVL                 QALKTY+NHQFTL
Sbjct: 181 SNESSNDKDWFKGSIFLIIATLAWSSLFVLQVYIYIYIYIYVYACVNQALKTYENHQFTL 240

Query: 241 TALVCFLGTLQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRG 300
           T L+CF+GTLQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKK+G
Sbjct: 241 TTLMCFVGTLQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKKG 300

Query: 301 PVFATAFSPLMMIIVAIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSA 360
           PVFATAFSPLMMIIVAIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKHKE+LE K S 
Sbjct: 301 PVFATAFSPLMMIIVAIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKESLENKLSG 360

Query: 361 ADD-EIPEAIKAS--SQPNIN----NNSIFISMPAPEDPIKPNQIP 373
           +DD EIPE IKAS  SQPN N    NN+IFISMP PE+PIKPNQ+P
Sbjct: 361 SDDNEIPEVIKASSNSQPNTNNNNTNNTIFISMPTPENPIKPNQMP 406

BLAST of HG10015048 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 3.3e-134
Identity = 253/396 (63.89%), Postives = 307/396 (77.53%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           M    ++ C  FL +SKPYFAMISLQFGYAGMNII+K+SL+ GMSHYVLVVYRHA ATA 
Sbjct: 1   MSMEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAV 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFER+ QP+IT  +F+Q+F+L LLGPVIDQNFYY GLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHL---- 180
           MT        ME L++KKL CQAK+ GT+V+VAGAMLMT+YKGP++++ W+K  H+    
Sbjct: 121 MTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSS 180

Query: 181 ----TNSNESSNNKDWFKGSIFLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGT 240
               T+S  SS++K++ KGSI LI ATLAWASLFVLQA  LKTY  HQ +LT L+CF+GT
Sbjct: 181 HANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGT 240

Query: 241 LQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSP 300
           LQA+AVT V EH  S WRIGWDMNLLAAAY+GIV SSISYYVQG+VMKKRGPVFATAFSP
Sbjct: 241 LQAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSP 300

Query: 301 LMMIIVAIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKEN----LEIKTSAADDEI 360
           LMM+IVA+MGSF+L+EKIFLGG+IG++LIV GLY+VLWGK KEN     E+    ++ ++
Sbjct: 301 LMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKV 360

Query: 361 PEAIKA-------SSQPNINNNSIFISMPAPEDPIK 368
            E ++A       S   N   ++I IS+P  E  +K
Sbjct: 361 TEDVEANGSKMKISEGDNSMLSTIVISVPLSETHLK 396

BLAST of HG10015048 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.9e-97
Identity = 189/346 (54.62%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++K  L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------MEK 133
            ++T  +F++IF+LALLGPVIDQN YY GLK TSPTFS A+SN++PA+T        MEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 134 LEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSNNKDWFKGSI 193
           +EM+K+RC  KVMGTLV+V G++LM  YKGP +       SHLT ++ S    D+ K ++
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF---RSHLTAAS-SPPTADYLKAAV 186

Query: 194 FLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVTLVAEHKASVWRIGW 253
           FL++A+L+WAS FVLQA  LK Y  H  +++ +VCF+GTLQ++A+  V EH  S   IG+
Sbjct: 187 FLLLASLSWASFFVLQAATLKKYSAH-LSMSTMVCFMGTLQSLALAFVMEHNPSALNIGF 246

Query: 254 DMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEKIFLG 313
           DMNLLA+AYAGI++SSI+YYVQGL+M+++GPVF TAF+PL+++IV+IM  F+L + I+LG
Sbjct: 247 DMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLG 306

Query: 314 GIIGSILIVFGLYSVLWGKHKENLEIKTSAADDEIPEAIKASSQPN 350
           G+IG ++++ G+Y+VLWGKH ++   +T   D+ +  A+K  S  N
Sbjct: 307 GVIGVVVLMVGVYAVLWGKHVDDDGEETRHEDNVV--AVKCCSGNN 345

BLAST of HG10015048 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.4e-94
Identity = 186/332 (56.02%), Postives = 253/332 (76.20%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++KV L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------MEK 133
           P++T  +F+QIFVLALLGP+IDQN YYAGLK TSPTF+ A++N++PA+T        MEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 134 LEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSNNKDWFKGSI 193
           +EM+K+R QAKV+GTLV V GAMLM L+K PL+       SHLT    S   +D+ K ++
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFL---RSHLTGHALSPAGEDYLKATV 186

Query: 194 FLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVTLVAEHKASVWRIGW 253
           FL+IA+ +WAS FVLQA  LK Y +H  +L+ +VCF+GTLQ+ A+T V E   S W IG+
Sbjct: 187 FLLIASFSWASFFVLQAATLKRYSSH-LSLSTMVCFMGTLQSTALTFVMEPNLSAWNIGF 246

Query: 254 DMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEKIFLG 313
           DMNLLA+AYAGI++SSI+YYVQG++ K++  +F TAF+PL++II +I+G  IL++ + LG
Sbjct: 247 DMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLILNQTLNLG 306

Query: 314 GIIGSILIVFGLYSVLWGK-----HKENLEIK 331
           G++G  ++V G+ +VLWGK      +EN+E K
Sbjct: 307 GVLGMAILVVGVCTVLWGKEGDIDEEENIEEK 334

BLAST of HG10015048 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.7e-83
Identity = 173/374 (46.26%), Postives = 248/374 (66.31%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           L+  KP+  ++SLQFGYAG++II+K +L++GMS +VL  YRH  AT  IAPF  F +R+ 
Sbjct: 3   LKTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKI 62

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------ME 132
           +P++TL +F +I +L LL P IDQN YY G+KYTS TF+ AM+N+LPA          +E
Sbjct: 63  RPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLE 122

Query: 133 KLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSN--NKDWFK 192
           K+ +KK+  QAK++GT+V+V GAMLMT+ KGPL+ + W+ N H  + + S+    +D  K
Sbjct: 123 KVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA-NPHDIHQDSSNTGVKQDLTK 182

Query: 193 GSIFLIIATLAWASLFVLQALKTYKNH--QFTLTALVCFLGTLQAIAVTLVAEH-KASVW 252
           G+  + I  + WA    LQA+ T K++  + +LTA +CFLG++++  V L  E    S W
Sbjct: 183 GASLIAIGCICWAGFINLQAI-TLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAW 242

Query: 253 RIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEK 312
            I  D  LLAA Y G++ S I YYVQG++MK RGPVF TAF+PL M+IVAI+GS IL+E 
Sbjct: 243 AIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEV 302

Query: 313 IFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADDEIPEAI-------KASSQPNINN 367
           +FLG I+G+I+IV GLYSVLWGK K+      S  D E+P +        KA+++ + N+
Sbjct: 303 MFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSKANAKMDTND 362

BLAST of HG10015048 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.7e-83
Identity = 165/348 (47.41%), Postives = 234/348 (67.24%), Query Frame = 0

Query: 11  GFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFER 70
           G + + KPY AMIS+QFGYAGM II+ VSL  GM+HYVL VYRHA ATA IAPF LF ER
Sbjct: 4   GLMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHER 63

Query: 71  RGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT-------- 130
           + +P++T ++F+QI +L  + PV+DQN YY G+ YTS TF+ A +N+LPA+T        
Sbjct: 64  KIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFR 123

Query: 131 MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMA-----------WSKNSHLTNS 190
           +E +  KK+R  AKV+GT+++V+GA+LMTLYKGP++               +  SH   +
Sbjct: 124 LESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSH-GGA 183

Query: 191 NESSNNKDWFKGSIFLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVT 250
             ++ +K W  G++ L+  T  WA  F+LQ+  LK Y   + +LT L+C +GTL+  AV+
Sbjct: 184 GAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYP-AELSLTTLICLMGTLEGTAVS 243

Query: 251 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 310
           LV     S W+IG+D NL AAAY+G++ S ++YYVQG+VM++RGPVF   F+PL ++I A
Sbjct: 244 LVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITA 303

Query: 311 IMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADDE 338
            +G  +LSE I LG +IG++ I+ GLY+V+WGK K+    K    DDE
Sbjct: 304 ALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKD----KRMTDDDE 345

BLAST of HG10015048 vs. ExPASy TrEMBL
Match: A0A1S3BDW4 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488823 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.4e-182
Identity = 349/388 (89.95%), Postives = 357/388 (92.01%), Query Frame = 0

Query: 1   MGKL--SLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60
           MGKL    NCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT
Sbjct: 1   MGKLISFFNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFAT 60

Query: 61  ASIAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120
           ASIAPFVLFFERRGQPR+T KVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML
Sbjct: 61  ASIAPFVLFFERRGQPRMTFKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNML 120

Query: 121 PAMT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTN 180
           PAMT        MEKLEMKKLRCQAKV+GTLV+V GAMLMTLYKGP LQM WSKNSHLTN
Sbjct: 121 PAMTFVMAVIFRMEKLEMKKLRCQAKVLGTLVTVGGAMLMTLYKGPPLQMPWSKNSHLTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
           S+ESSNNKDWFKGSIFLIIATLAWASLFVL  Q LKTYKNHQFTLT LVCF+GTLQAIAV
Sbjct: 181 SSESSNNKDWFKGSIFLIIATLAWASLFVLQNQVLKTYKNHQFTLTTLVCFMGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV
Sbjct: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKT-SAADDEIPEAIKAS-- 360
           AIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKHKENLE K+ S  D+EIPE IKAS  
Sbjct: 301 AIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHKENLESKSGSDHDNEIPEVIKASNN 360

Query: 361 SQPNI-NNNSIFISMPAPEDPIKPNQIP 373
           SQPNI NNNSIFISMP PE P KPNQIP
Sbjct: 361 SQPNISNNNSIFISMPTPETPTKPNQIP 388

BLAST of HG10015048 vs. ExPASy TrEMBL
Match: A0A6J1CEJ4 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111010012 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 9.2e-180
Identity = 342/402 (85.07%), Postives = 364/402 (90.55%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSL+CCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPFVLFFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFVLFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNS--HLTN 180
           MT        ME L+MKKLRCQAK++GTLV+VAGAMLMTLYKGPL+QMAWSK+S    TN
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKILGTLVTVAGAMLMTLYKGPLVQMAWSKHSSNSSTN 180

Query: 181 SNESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
           S+E+S NKDWFKGSIFLIIATLAWASLFVL  QALKTYKNHQFTLT LVCFLGTLQAIAV
Sbjct: 181 SDENSANKDWFKGSIFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFLGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV
Sbjct: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIK--TSAADDEIPEAIKASS 360
           AIMGSFIL+EKIFLGGIIGSILIVFGLYSVLWGKH+ENLEIK  ++AADDEIPEAIKA+S
Sbjct: 301 AIMGSFILAEKIFLGGIIGSILIVFGLYSVLWGKHRENLEIKSASAAADDEIPEAIKANS 360

Query: 361 QPNIN--------------NNSIFISMPAPEDPIKPNQIPRE 375
            PN+               +NS+FI++  PE+PIKPNQ+PRE
Sbjct: 361 NPNLQPNNNNNGNMGPNKPSNSLFITISDPENPIKPNQVPRE 402

BLAST of HG10015048 vs. ExPASy TrEMBL
Match: A0A6J1GYG8 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458661 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 8.4e-173
Identity = 333/394 (84.52%), Postives = 352/394 (89.34%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLS +    FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSFS----FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTN-S 180
           MT        ME LEMKKLRCQAKV+GTLV+V GAM+MTLYKGPL+QM WSK+SH +N +
Sbjct: 121 MTFVMAVIFRMENLEMKKLRCQAKVLGTLVTVGGAMVMTLYKGPLVQMPWSKHSHHSNQA 180

Query: 181 NESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAVT 240
           ++   NKDWFKGS+FLIIATLAWASLFVL  QALKTYKNHQFTLT LVCF+GTLQAIAV 
Sbjct: 181 SDDKANKDWFKGSMFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFVGTLQAIAVA 240

Query: 241 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300
           LVAEHKA VWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA
Sbjct: 241 LVAEHKAVVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300

Query: 301 IMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAA----DDEIPEAIKAS 360
           IMGSFIL+EKIFLGG+IGSI IVFGLYSVLWGKH+ENLEIK +AA    D+EIP+ IK  
Sbjct: 301 IMGSFILAEKIFLGGLIGSIFIVFGLYSVLWGKHRENLEIKVAAAAASSDEEIPQPIKPP 360

Query: 361 SQPNIN-----NNSIFISMPAPEDPIKPNQIPRE 375
           SQPN N     NNSIFISMPAPE+PIKPNQIPRE
Sbjct: 361 SQPNNNNNNIANNSIFISMPAPENPIKPNQIPRE 390

BLAST of HG10015048 vs. ExPASy TrEMBL
Match: A0A6J1J918 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111483690 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.4e-172
Identity = 331/396 (83.59%), Postives = 353/396 (89.14%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLS +    FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSFS----FLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           I PFVLFFERRGQP+ITLK+FIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IVPFVLFFERRGQPKITLKIFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTN-S 180
           MT        ME L+MKKLRCQAKV+GTLV+V GAM+MTLYKGPL+QM WSK+SH +N +
Sbjct: 121 MTFVMAVIFRMENLDMKKLRCQAKVLGTLVTVGGAMVMTLYKGPLVQMPWSKHSHHSNQA 180

Query: 181 NESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAVT 240
           ++   NKDWFKGS+FLIIATLAWASLFVL  QALKTYKNHQFTLT LVCF+GTLQAIAVT
Sbjct: 181 SDDKANKDWFKGSMFLIIATLAWASLFVLQNQALKTYKNHQFTLTTLVCFVGTLQAIAVT 240

Query: 241 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300
           LVAEHKA+VWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA
Sbjct: 241 LVAEHKAAVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 300

Query: 301 IMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAA----DDEIPEAIKAS 360
           IMGSFIL+E IFLGGIIGSI IVFGLYSVLWGKH+ENLEIK +AA    D+EIP+ IK  
Sbjct: 301 IMGSFILAENIFLGGIIGSIFIVFGLYSVLWGKHRENLEIKVAAAAASSDEEIPQPIKLP 360

Query: 361 SQPNIN-------NNSIFISMPAPEDPIKPNQIPRE 375
           SQPN N       NNSIFISMPAPE+PIKPNQIPRE
Sbjct: 361 SQPNNNNNNNNIANNSIFISMPAPENPIKPNQIPRE 392

BLAST of HG10015048 vs. ExPASy TrEMBL
Match: A0A6J1HPD7 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111466510 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 6.0e-171
Identity = 327/396 (82.58%), Postives = 350/396 (88.38%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           MGKLSL+C + F+ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS
Sbjct: 1   MGKLSLSCWSSFVENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFERRGQP+ITLKVF QIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERRGQPKITLKVFTQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNS- 180
           MT        MEKLEMKKLRCQAK +GTLV+V GAMLMTLYKGPL+QMAWSK+SH++ S 
Sbjct: 121 MTFMMALIFRMEKLEMKKLRCQAKFLGTLVTVVGAMLMTLYKGPLIQMAWSKHSHVSTSS 180

Query: 181 -NESSNNKDWFKGSIFLIIATLAWASLFVL--QALKTYKNHQFTLTALVCFLGTLQAIAV 240
            +++S NKDWFKGSIFLIIATLAW+SLFVL  QALKTY NHQ TLT LVC +GTLQAIAV
Sbjct: 181 NDQASANKDWFKGSIFLIIATLAWSSLFVLQNQALKTYMNHQLTLTTLVCLMGTLQAIAV 240

Query: 241 TLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300
           T+VAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV
Sbjct: 241 TVVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIV 300

Query: 301 AIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADD--EIPEAIKASS 360
           AIMGSFILSEKI+LGGIIGSILIVFGLYSVLWGKHKENLE K++  DD  ++P+AIK SS
Sbjct: 301 AIMGSFILSEKIYLGGIIGSILIVFGLYSVLWGKHKENLETKSAVDDDTHKLPQAIKLSS 360

Query: 361 QPNINN----------NSIFISMPAPEDPIKPNQIP 373
           QPN NN          NS+ ISMP PE PIKPNQ P
Sbjct: 361 QPNNNNNLNLEPNKLLNSLIISMPDPESPIKPNQNP 396

BLAST of HG10015048 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.4e-135
Identity = 253/396 (63.89%), Postives = 307/396 (77.53%), Query Frame = 0

Query: 1   MGKLSLNCCNGFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATAS 60
           M    ++ C  FL +SKPYFAMISLQFGYAGMNII+K+SL+ GMSHYVLVVYRHA ATA 
Sbjct: 1   MSMEEISSCESFLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAV 60

Query: 61  IAPFVLFFERRGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPA 120
           IAPF  FFER+ QP+IT  +F+Q+F+L LLGPVIDQNFYY GLKYTSPTFSCAMSNMLPA
Sbjct: 61  IAPFAFFFERKAQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPA 120

Query: 121 MT--------MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHL---- 180
           MT        ME L++KKL CQAK+ GT+V+VAGAMLMT+YKGP++++ W+K  H+    
Sbjct: 121 MTFILAVLFRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSS 180

Query: 181 ----TNSNESSNNKDWFKGSIFLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGT 240
               T+S  SS++K++ KGSI LI ATLAWASLFVLQA  LKTY  HQ +LT L+CF+GT
Sbjct: 181 HANTTSSKNSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGT 240

Query: 241 LQAIAVTLVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSP 300
           LQA+AVT V EH  S WRIGWDMNLLAAAY+GIV SSISYYVQG+VMKKRGPVFATAFSP
Sbjct: 241 LQAVAVTFVMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSP 300

Query: 301 LMMIIVAIMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKEN----LEIKTSAADDEI 360
           LMM+IVA+MGSF+L+EKIFLGG+IG++LIV GLY+VLWGK KEN     E+    ++ ++
Sbjct: 301 LMMVIVAVMGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKV 360

Query: 361 PEAIKA-------SSQPNINNNSIFISMPAPEDPIK 368
            E ++A       S   N   ++I IS+P  E  +K
Sbjct: 361 TEDVEANGSKMKISEGDNSMLSTIVISVPLSETHLK 396

BLAST of HG10015048 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-98
Identity = 189/346 (54.62%), Postives = 263/346 (76.01%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++K  L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------MEK 133
            ++T  +F++IF+LALLGPVIDQN YY GLK TSPTFS A+SN++PA+T        MEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 134 LEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSNNKDWFKGSI 193
           +EM+K+RC  KVMGTLV+V G++LM  YKGP +       SHLT ++ S    D+ K ++
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF---RSHLTAAS-SPPTADYLKAAV 186

Query: 194 FLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVTLVAEHKASVWRIGW 253
           FL++A+L+WAS FVLQA  LK Y  H  +++ +VCF+GTLQ++A+  V EH  S   IG+
Sbjct: 187 FLLLASLSWASFFVLQAATLKKYSAH-LSMSTMVCFMGTLQSLALAFVMEHNPSALNIGF 246

Query: 254 DMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEKIFLG 313
           DMNLLA+AYAGI++SSI+YYVQGL+M+++GPVF TAF+PL+++IV+IM  F+L + I+LG
Sbjct: 247 DMNLLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLG 306

Query: 314 GIIGSILIVFGLYSVLWGKHKENLEIKTSAADDEIPEAIKASSQPN 350
           G+IG ++++ G+Y+VLWGKH ++   +T   D+ +  A+K  S  N
Sbjct: 307 GVIGVVVLMVGVYAVLWGKHVDDDGEETRHEDNVV--AVKCCSGNN 345

BLAST of HG10015048 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.4e-95
Identity = 186/332 (56.02%), Postives = 253/332 (76.20%), Query Frame = 0

Query: 14  ENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRGQ 73
           E++KPYFAM+ LQFGYAGMN+++KV L RGMSHYVLV YR+AFATA+IAPF L  ER+ +
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 74  PRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------MEK 133
           P++T  +F+QIFVLALLGP+IDQN YYAGLK TSPTF+ A++N++PA+T        MEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 134 LEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSNNKDWFKGSI 193
           +EM+K+R QAKV+GTLV V GAMLM L+K PL+       SHLT    S   +D+ K ++
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFL---RSHLTGHALSPAGEDYLKATV 186

Query: 194 FLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVTLVAEHKASVWRIGW 253
           FL+IA+ +WAS FVLQA  LK Y +H  +L+ +VCF+GTLQ+ A+T V E   S W IG+
Sbjct: 187 FLLIASFSWASFFVLQAATLKRYSSH-LSLSTMVCFMGTLQSTALTFVMEPNLSAWNIGF 246

Query: 254 DMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEKIFLG 313
           DMNLLA+AYAGI++SSI+YYVQG++ K++  +F TAF+PL++II +I+G  IL++ + LG
Sbjct: 247 DMNLLASAYAGIMSSSIAYYVQGMMTKQKSVIFVTAFNPLVVIIGSIIGFLILNQTLNLG 306

Query: 314 GIIGSILIVFGLYSVLWGK-----HKENLEIK 331
           G++G  ++V G+ +VLWGK      +EN+E K
Sbjct: 307 GVLGMAILVVGVCTVLWGKEGDIDEEENIEEK 334

BLAST of HG10015048 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-84
Identity = 165/348 (47.41%), Postives = 234/348 (67.24%), Query Frame = 0

Query: 11  GFLENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFER 70
           G + + KPY AMIS+QFGYAGM II+ VSL  GM+HYVL VYRHA ATA IAPF LF ER
Sbjct: 4   GLMNSLKPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHER 63

Query: 71  RGQPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT-------- 130
           + +P++T ++F+QI +L  + PV+DQN YY G+ YTS TF+ A +N+LPA+T        
Sbjct: 64  KIRPKMTFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFR 123

Query: 131 MEKLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMA-----------WSKNSHLTNS 190
           +E +  KK+R  AKV+GT+++V+GA+LMTLYKGP++               +  SH   +
Sbjct: 124 LESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSH-GGA 183

Query: 191 NESSNNKDWFKGSIFLIIATLAWASLFVLQA--LKTYKNHQFTLTALVCFLGTLQAIAVT 250
             ++ +K W  G++ L+  T  WA  F+LQ+  LK Y   + +LT L+C +GTL+  AV+
Sbjct: 184 GAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYP-AELSLTTLICLMGTLEGTAVS 243

Query: 251 LVAEHKASVWRIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVA 310
           LV     S W+IG+D NL AAAY+G++ S ++YYVQG+VM++RGPVF   F+PL ++I A
Sbjct: 244 LVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPLCVVITA 303

Query: 311 IMGSFILSEKIFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADDE 338
            +G  +LSE I LG +IG++ I+ GLY+V+WGK K+    K    DDE
Sbjct: 304 ALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKD----KRMTDDDE 345

BLAST of HG10015048 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-84
Identity = 173/374 (46.26%), Postives = 248/374 (66.31%), Query Frame = 0

Query: 13  LENSKPYFAMISLQFGYAGMNIISKVSLSRGMSHYVLVVYRHAFATASIAPFVLFFERRG 72
           L+  KP+  ++SLQFGYAG++II+K +L++GMS +VL  YRH  AT  IAPF  F +R+ 
Sbjct: 3   LKTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKI 62

Query: 73  QPRITLKVFIQIFVLALLGPVIDQNFYYAGLKYTSPTFSCAMSNMLPAMT--------ME 132
           +P++TL +F +I +L LL P IDQN YY G+KYTS TF+ AM+N+LPA          +E
Sbjct: 63  RPKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLE 122

Query: 133 KLEMKKLRCQAKVMGTLVSVAGAMLMTLYKGPLLQMAWSKNSHLTNSNESSN--NKDWFK 192
           K+ +KK+  QAK++GT+V+V GAMLMT+ KGPL+ + W+ N H  + + S+    +D  K
Sbjct: 123 KVNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWA-NPHDIHQDSSNTGVKQDLTK 182

Query: 193 GSIFLIIATLAWASLFVLQALKTYKNH--QFTLTALVCFLGTLQAIAVTLVAEH-KASVW 252
           G+  + I  + WA    LQA+ T K++  + +LTA +CFLG++++  V L  E    S W
Sbjct: 183 GASLIAIGCICWAGFINLQAI-TLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAW 242

Query: 253 RIGWDMNLLAAAYAGIVTSSISYYVQGLVMKKRGPVFATAFSPLMMIIVAIMGSFILSEK 312
            I  D  LLAA Y G++ S I YYVQG++MK RGPVF TAF+PL M+IVAI+GS IL+E 
Sbjct: 243 AIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEV 302

Query: 313 IFLGGIIGSILIVFGLYSVLWGKHKENLEIKTSAADDEIPEAI-------KASSQPNINN 367
           +FLG I+G+I+IV GLYSVLWGK K+      S  D E+P +        KA+++ + N+
Sbjct: 303 MFLGRILGAIVIVLGLYSVLWGKSKDEPSSSFSDMDKELPLSTPQIVLPSKANAKMDTND 362