HG10014909 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014909
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Radical SAM superfamily protein
Location	Chr02: 21702251 .. 21703917 (+)
RNA-Seq Expression	HG10014909
Synteny	HG10014909

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCGTGGGAACTTGGGAAGGCTGGCCTTAAAGTTCAACCAGGTTTACTACATGCTAATTTTTTGTTGCTGAGTGTACAATTTCTCATCAATTCTTTATATTTTTTCAAGGATGTTCAAATTGGACAAAGAATGATTACAATTTCTACGGTGGGGGTTCCTAACACAATTAAAAGGCTGGCTTCTCACAAGCTTCAGTCAACATTGGCTGTCAGGTAACATATATTCTACAGGAAAAGCATTCTTTTAGATTGTTTGATTATTTATTTATGATTATTATTTTTTGAAAAAAGAGAACCATTGTCAGTGATATCTTGCCGAGGGAAAAGTTTGCTAGTTTCCGTATGGCATATACTAGTGACAGATAAATTATATGTACGGTTTAGAAGTGTAGGATTCTTTTTTTCTTCAGTTAATGTTTATTGGCTGGTTATATGTTCTCATCCCTAAATTTTTAATCGGTAGCAGTTAGTTGAACCTGATCTAAGAACTGTGCTAATTGGAGAATTTTGGCACCCCAAATATCTGAGTAGAGAGGTTCATGTATAAAGTGATCGATTGCTTTCTTGCAGTTATCTCATTAAATTCTGTTAAATTTAGGAGTTAAATAACTGATATCTCTCTGATTCTAACTTTCATTTGAAGAGTTTATATATAAATAATTTAAGAAATAAAATTGAGTAATAAAGAAGACATGCAAAAGAGGGAGAGGAGAAATCCTCCCAAATCCCCAAGAGCTTGCACAAAGCTTTCCCAGGTGGTATGAATTATAACCACAGAATGACTACAGAATCGTTAAGAAATTATACTAGGCAGACCCGTTCTTGAAGAGTTTTATCTTGCATCTCAAAATTTTCCTCTCCCAATCCGTAAGTTCCAAAGGTTAGCATAGGTTGTTTTGAAGAGGTGATCCATGGGAATTTGTAGGACGAGGTCCTTAGATTTTGGCTCTCTTTTATATTTTAGTTTGATTAACTCTTCATTTTATGCCCATCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCAATTATGAAAGATTGTCGTGATTATTTCCTCCAAACTAATCGGAGAGTATCGTTCGAGTATGCACTTTTAGGTACTGGCATCACTTTAGCAATACAATCCTCATCTCTCCTTTCATTGTGTTTCTCACTGTAGTTCGGGCATCGTACATCTGACAAATTGTTTTCCCCCCAATTTTTCGGTTTATCATTTGAACAGCTGGAGTCAATGATGCAGTAGAGCATGCTGTAGAACTTGCAAAGCTTCTTCATGAATGGGGAGGTGGCTATCACGTGAACCTGATACCTTTCAATCCCGTAGAAGGCTCTGAGTATCAGCGTCCATACAAGAAAGCTGTATGTTCTCGACAGACGTGCTAATGTTTCGTATTATAAACGTATATTGTTGTCTTCACTAATCCATAATCTTTTCTTCTATTTTCAGGTGATGGCATTTGTAGCTGCTCTAGAGTCCCATAAAATCACTGTAAGCGTTCGTCAAACAAGAGGTCTCGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTTCAAAAGAACCCTTTGCTTCTCGATTCCGACATCAGTCAGTCTGAATCAGCTGTAGCTGTGGCATGTTGA

mRNA sequence

ATGGCCTCGTGGGAACTTGGGAAGGCTGGCCTTAAAGTTCAACCAGGTTTACTACATGCTAATTTTTTGTTGCTGAGTGTACAATTTCTCATCAATTCTTTATATTTTTTCAAGGATGTTCAAATTGGACAAAGAATGATTACAATTTCTACGGTGGGGGTTCCTAACACAATTAAAAGGCTGGCTTCTCACAAGCTTCAGTCAACATTGGCTGTCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCAATTATGAAAGATTGTCGTGATTATTTCCTCCAAACTAATCGGAGAGTATCGTTCGAGTATGCACTTTTAGCTGGAGTCAATGATGCAGTAGAGCATGCTGTAGAACTTGCAAAGCTTCTTCATGAATGGGGAGGTGGCTATCACGTGAACCTGATACCTTTCAATCCCGTAGAAGGCTCTGAGTATCAGCGTCCATACAAGAAAGCTGTGATGGCATTTGTAGCTGCTCTAGAGTCCCATAAAATCACTGTAAGCGTTCGTCAAACAAGAGGTCTCGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTTCAAAAGAACCCTTTGCTTCTCGATTCCGACATCAGTCAGTCTGAATCAGCTGTAGCTGTGGCATGTTGA

Coding sequence (CDS)

ATGGCCTCGTGGGAACTTGGGAAGGCTGGCCTTAAAGTTCAACCAGGTTTACTACATGCTAATTTTTTGTTGCTGAGTGTACAATTTCTCATCAATTCTTTATATTTTTTCAAGGATGTTCAAATTGGACAAAGAATGATTACAATTTCTACGGTGGGGGTTCCTAACACAATTAAAAGGCTGGCTTCTCACAAGCTTCAGTCAACATTGGCTGTCAGCTTACATGCACCAAACCAGAAGCTTCGGGAGACTATTGTGCCCAGTGCAAAAGCATATCCTCTTGAAGCAATTATGAAAGATTGTCGTGATTATTTCCTCCAAACTAATCGGAGAGTATCGTTCGAGTATGCACTTTTAGCTGGAGTCAATGATGCAGTAGAGCATGCTGTAGAACTTGCAAAGCTTCTTCATGAATGGGGAGGTGGCTATCACGTGAACCTGATACCTTTCAATCCCGTAGAAGGCTCTGAGTATCAGCGTCCATACAAGAAAGCTGTGATGGCATTTGTAGCTGCTCTAGAGTCCCATAAAATCACTGTAAGCGTTCGTCAAACAAGAGGTCTCGATGCAAATGCAGCTTGTGGTCAGCTAAGAAACCAATTTCAAAAGAACCCTTTGCTTCTCGATTCCGACATCAGTCAGTCTGAATCAGCTGTAGCTGTGGCATGTTGA

Protein sequence

MASWELGKAGLKVQPGLLHANFLLLSVQFLINSLYFFKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSESAVAVAC

Homology

[Close]

BLAST of HG10014909 vs. NCBI nr
Match: XP_038892136.1 (probable dual-specificity RNA methyltransferase RlmN [Benincasa hispida])

HSP 1 Score: 363.6 bits (932), Expect = 1.2e-96
Identity = 181/186 (97.31%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+
Sbjct: 232 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAL 291

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYFLQT+RRVSFEYALLAGVNDAVEHAVEL+KLLHEWGGGYHVNLIPFNP+EGSE
Sbjct: 292 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELSKLLHEWGGGYHVNLIPFNPIEGSE 351

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL+DSDISQSES
Sbjct: 352 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQSES 411

Query: 218 AVAVAC 224
           AVAVAC
Sbjct: 412 AVAVAC 417

[Close]

BLAST of HG10014909 vs. NCBI nr
Match: KAA0050298.1 (putative dual-specificity RNA methyltransferase RlmN [Cucumis melo var. makuwa] >TYK03517.1 putative dual-specificity RNA methyltransferase RlmN [Cucumis melo var. makuwa])

HSP 1 Score: 360.9 bits (925), Expect = 7.8e-96
Identity = 180/193 (93.26%), Postives = 188/193 (97.41%), Query Frame = 0


Query: 28  QFLINSLYFFKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP 87
           + L+NS YF KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP
Sbjct: 360 KLLMNSSYFLKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP 419

Query: 88  SAKAYPLEAIMKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL 147
           SAKAYPLEA+MKDC DYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL
Sbjct: 420 SAKAYPLEALMKDCHDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL 479

Query: 148 IPFNPVEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL 207
           IPFNP+EGSEYQRPYKKAVMAFVAALESHKITVSVR+TRGLDANAACGQLRNQFQKNPLL
Sbjct: 480 IPFNPIEGSEYQRPYKKAVMAFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLL 539

Query: 208 LDSDISQSESAVA 221
           +DSDISQSE+A A
Sbjct: 540 VDSDISQSEAAEA 552

[Close]

BLAST of HG10014909 vs. NCBI nr
Match: XP_022989725.1 (uncharacterized protein LOC111486721 [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 5.1e-95
Identity = 180/186 (96.77%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLL EWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQSES
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSES 410

Query: 218 AVAVAC 224
           AVAVAC
Sbjct: 411 AVAVAC 416

[Close]

BLAST of HG10014909 vs. NCBI nr
Match: XP_008461658.1 (PREDICTED: probable dual-specificity RNA methyltransferase RlmN [Cucumis melo])

HSP 1 Score: 357.8 bits (917), Expect = 6.6e-95
Identity = 178/186 (95.70%), Postives = 184/186 (98.92%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAL 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDC DYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCHDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVR+TRGLDANAACGQLRNQFQKNPLL+DSDISQSE+
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLLVDSDISQSEA 410

Query: 218 AVAVAC 224
           A AVAC
Sbjct: 411 AEAVAC 416

[Close]

BLAST of HG10014909 vs. NCBI nr
Match: KAG6601003.1 (hypothetical protein SDJN03_06236, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 179/186 (96.24%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYF+QT+RRVSFEYALLAGVNDAVEHAVELAKLL EWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCRDYFVQTSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQSES
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSES 410

Query: 218 AVAVAC 224
           AVAVAC
Sbjct: 411 AVAVAC 416

[Close]

BLAST of HG10014909 vs. ExPASy Swiss-Prot
Match: Q2JRQ8 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=rlmN PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.9e-50
Identity = 101/167 (60.48%), Postives = 123/167 (73.65%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           +D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ+LR+ ++PSA  YPL+A+
Sbjct: 178 QDIGIGQRHITLSTVGVPRQIPWLAQQDLQITLAVSLHAPNQELRQQLIPSAAHYPLDAL 237

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLH---EWGGGYHVNLIPFNPVE 157
           ++DCRDY L + RR+SFEY LLAGVND   HA +LA LL    + G   HVNLIP+NP+ 
Sbjct: 238 IQDCRDYMLCSGRRISFEYTLLAGVNDLPIHARQLAHLLRQASQAGARLHVNLIPYNPIA 297

Query: 158 GSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQF 202
            ++YQRP+   V  FV  LE H + VSVRQTRGLD+NAACGQLR  F
Sbjct: 298 EADYQRPHPTRVAEFVRLLEEHHVQVSVRQTRGLDSNAACGQLRGSF 344

[Close]

BLAST of HG10014909 vs. ExPASy Swiss-Prot
Match: Q2JMN2 (Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=rlmN PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-49
Identity = 99/170 (58.24%), Postives = 124/170 (72.94%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           +D+ IGQR IT+STVGVP  I  LA   LQ TLAVSLHAPNQ LR+ ++PSA  YPL+ +
Sbjct: 178 QDIGIGQRHITLSTVGVPRQIAWLAQQDLQVTLAVSLHAPNQDLRQRLIPSASHYPLDTL 237

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHE---WGGGYHVNLIPFNPVE 157
           ++DCRDY L+T RRVSFEY LL+GVND   HA +LA+LL +    G   HVNLIP+NP+ 
Sbjct: 238 IQDCRDYMLRTGRRVSFEYTLLSGVNDLPIHARQLAQLLQQASRSGVQLHVNLIPYNPIS 297

Query: 158 GSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKN 205
            ++YQRP+   V  FV  LE H++  +VRQTRGLD NAACGQLR  F ++
Sbjct: 298 EADYQRPHPTRVREFVRQLEQHQVRATVRQTRGLDGNAACGQLRGSFLRS 347

[Close]

BLAST of HG10014909 vs. ExPASy Swiss-Prot
Match: Q7NIV3 (Probable dual-specificity RNA methyltransferase RlmN OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=rlmN PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 4.4e-49
Identity = 99/162 (61.11%), Postives = 120/162 (74.07%), Query Frame = 0


Query: 39  DVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIM 98
           D+ IGQR IT+STVGVP  I+RL ++KLQ TLAVSLHAPNQ LR  ++P+A+ YP+E ++
Sbjct: 176 DIGIGQRQITVSTVGVPGQIRRLGTYKLQITLAVSLHAPNQDLRLKLIPTAQHYPIEELL 235

Query: 99  KDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSEY 158
           +DCRDY   TNRRVSFEY LLAG+ND   HA ELA +L   G   HVNLIP+NP+EG EY
Sbjct: 236 EDCRDYVETTNRRVSFEYTLLAGINDEPHHARELAAILR--GFQSHVNLIPYNPIEGVEY 295

Query: 159 QRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQ 201
           +RP +  V AF   L  HKI  SVR TRGL+  AACGQLR +
Sbjct: 296 ERPGEARVRAFERELVRHKIAASVRHTRGLEEAAACGQLRRR 335

[Close]

BLAST of HG10014909 vs. ExPASy Swiss-Prot
Match: B0C9F4 (Probable dual-specificity RNA methyltransferase RlmN OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=rlmN PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.8e-48
Identity = 95/161 (59.01%), Postives = 122/161 (75.78%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           +DV IGQR +T+STVG+P  I +LA H+LQ TLAVSLHA NQ++R  +VPSAK YPLE +
Sbjct: 183 QDVGIGQRSLTVSTVGIPKQILKLAQHQLQITLAVSLHASNQRIRTQLVPSAKHYPLEKL 242

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           +KDCR Y  QT RRV+FEY +L+GVND  EHA+ELA   H  G   HVNLIP+NP+   +
Sbjct: 243 LKDCRAYVTQTGRRVTFEYIVLSGVNDQTEHALELAH--HLRGFQSHVNLIPYNPISEVD 302

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLR 199
           YQRP +K +  F+ +L+S  IT S+R++RGLD +AACGQLR
Sbjct: 303 YQRPTQKQLQQFLNSLQSQHITASIRRSRGLDKDAACGQLR 341

[Close]

BLAST of HG10014909 vs. ExPASy Swiss-Prot
Match: B7K4N4 (Probable dual-specificity RNA methyltransferase RlmN OS=Rippkaea orientalis (strain PCC 8801) OX=41431 GN=rlmN PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 4.8e-48
Identity = 100/176 (56.82%), Postives = 129/176 (73.30%), Query Frame = 0


Query: 24  LLSVQFLINSLYFF-KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLR 83
           LL+++ ++ S+    +D+ IGQR +TISTVG+P  I +LA H LQ TLAVSLHA NQ LR
Sbjct: 163 LLNLEEVVKSVKILNQDIGIGQRSLTISTVGLPQKIIQLAHHHLQVTLAVSLHASNQPLR 222

Query: 84  ETIVPSAKAYPLEAIMKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGG 143
           ET++PSA+ Y L+ ++ DCR+Y   T RR+SFEY LL GVND  E A+ELA LL   G  
Sbjct: 223 ETLIPSAQHYTLKNLLADCREYVNITGRRISFEYVLLGGVNDLPEQAIELANLLK--GFQ 282

Query: 144 YHVNLIPFNPVEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLR 199
            HVNLIP+NP++ ++YQRP +  +  FV  LE HKI VSVR +RGL+ANAACGQLR
Sbjct: 283 SHVNLIPYNPIDEADYQRPNQTQIQTFVQVLEQHKIAVSVRYSRGLEANAACGQLR 336

[Close]

BLAST of HG10014909 vs. ExPASy TrEMBL
Match: A0A5A7U4V1 (Putative dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00070 PE=4 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 3.8e-96
Identity = 180/193 (93.26%), Postives = 188/193 (97.41%), Query Frame = 0


Query: 28  QFLINSLYFFKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP 87
           + L+NS YF KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP
Sbjct: 360 KLLMNSSYFLKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVP 419

Query: 88  SAKAYPLEAIMKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL 147
           SAKAYPLEA+MKDC DYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL
Sbjct: 420 SAKAYPLEALMKDCHDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNL 479

Query: 148 IPFNPVEGSEYQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL 207
           IPFNP+EGSEYQRPYKKAVMAFVAALESHKITVSVR+TRGLDANAACGQLRNQFQKNPLL
Sbjct: 480 IPFNPIEGSEYQRPYKKAVMAFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLL 539

Query: 208 LDSDISQSESAVA 221
           +DSDISQSE+A A
Sbjct: 540 VDSDISQSEAAEA 552

[Close]

BLAST of HG10014909 vs. ExPASy TrEMBL
Match: A0A6J1JN65 (uncharacterized protein LOC111486721 OS=Cucurbita maxima OX=3661 GN=LOC111486721 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.5e-95
Identity = 180/186 (96.77%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLL EWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQSES
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSES 410

Query: 218 AVAVAC 224
           AVAVAC
Sbjct: 411 AVAVAC 416

[Close]

BLAST of HG10014909 vs. ExPASy TrEMBL
Match: A0A1S3CGI7 (probable dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103500206 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.2e-95
Identity = 178/186 (95.70%), Postives = 184/186 (98.92%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAL 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDC DYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCHDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVR+TRGLDANAACGQLRNQFQKNPLL+DSDISQSE+
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRRTRGLDANAACGQLRNQFQKNPLLVDSDISQSEA 410

Query: 218 AVAVAC 224
           A AVAC
Sbjct: 411 AEAVAC 416

[Close]

BLAST of HG10014909 vs. ExPASy TrEMBL
Match: A0A6J1GZJ0 (uncharacterized protein LOC111458934 OS=Cucurbita moschata OX=3662 GN=LOC111458934 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 5.5e-95
Identity = 179/186 (96.24%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI
Sbjct: 231 KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYF+QT+RRVSFEYALLAGVNDAVEHAVELAKLL EWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCRDYFVQTSRRVSFEYALLAGVNDAVEHAVELAKLLREWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL D+D SQSES
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLADTDSSQSES 410

Query: 218 AVAVAC 224
           AVAVAC
Sbjct: 411 AVAVAC 416

[Close]

BLAST of HG10014909 vs. ExPASy TrEMBL
Match: A0A0A0KLM9 (Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G181470 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.0e-93
Identity = 175/183 (95.63%), Postives = 181/183 (98.91%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KDVQIGQRMITISTVGVPNTI+RLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEA+
Sbjct: 231 KDVQIGQRMITISTVGVPNTIQRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAL 290

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYFLQT+RRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNP+EGSE
Sbjct: 291 MKDCRDYFLQTSRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPIEGSE 350

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSES 217
           YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLL+DSDISQ E+
Sbjct: 351 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLVDSDISQPEA 410

Query: 218 AVA 221
           A A
Sbjct: 411 AEA 413

[Close]

BLAST of HG10014909 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 3.0e-85
Identity = 156/187 (83.42%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KD++IGQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAI
Sbjct: 242 KDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAI 301

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYF +TNRRVSFEYALLAGVND VEHAVELA+LL EWG  YHVNLIP+NP+EGSE
Sbjct: 302 MKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSE 361

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSE- 217
           YQRPYKKAV+AF AALES KIT SVRQTRGLDA+AACGQLRN+FQK+PLL ++D  +S+ 
Sbjct: 362 YQRPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQP 421

Query: 218 SAVAVAC 224
            A AVAC
Sbjct: 422 DAEAVAC 428

[Close]

BLAST of HG10014909 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 3.0e-85
Identity = 156/187 (83.42%), Postives = 171/187 (91.44%), Query Frame = 0


Query: 38  KDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAI 97
           KD++IGQRMITISTVGVPNTIK+LASHKLQSTLAVSLHAPNQ LRE IVPSAKAYPLEAI
Sbjct: 245 KDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQSLREKIVPSAKAYPLEAI 304

Query: 98  MKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSE 157
           MKDCRDYF +TNRRVSFEYALLAGVND VEHAVELA+LL EWG  YHVNLIP+NP+EGSE
Sbjct: 305 MKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWGKTYHVNLIPYNPIEGSE 364

Query: 158 YQRPYKKAVMAFVAALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSDISQSE- 217
           YQRPYKKAV+AF AALES KIT SVRQTRGLDA+AACGQLRN+FQK+PLL ++D  +S+ 
Sbjct: 365 YQRPYKKAVLAFAAALESRKITASVRQTRGLDASAACGQLRNKFQKSPLLTETDSQESQP 424

Query: 218 SAVAVAC 224
            A AVAC
Sbjct: 425 DAEAVAC 431

[Close]

BLAST of HG10014909 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 9.4e-23
Identity = 64/179 (35.75%), Postives = 108/179 (60.34%), Query Frame = 0


Query: 21  NFLLLSVQFLINSLYFFKDVQIGQRMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQK 80
           N ++ +V+ ++N  +     Q+  + ITISTVG+ + I +L +     +LAVSLHAP Q+
Sbjct: 177 NAVVEAVRVMLNQPF-----QLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQE 236

Query: 81  LRETIVPSAKAYPLEAIMKDCRDYFLQTNRRVSFEYALLAGVNDAVEHAVELAKLLHEWG 140
           +R  I+P+A+A+PL+ +M   + +   + +++  EY +L GVND  +HA  L +LL  + 
Sbjct: 237 IRCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTF- 296

Query: 141 GGYHVNLIPFNPV-EGSEYQRPYKKAVMAFVAAL-ESHKITVSVRQTRGLDANAACGQL 198
               +NLIPFNP+   S+++    + V  F   L E++KI  ++R+  G D + ACGQL
Sbjct: 297 -QVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQL 348

[Close]

BLAST of HG10014909 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 4.5e-17
Identity = 55/154 (35.71%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 45  RMITISTVGVPNTIKRLASHKLQSTLAVSLHAPNQKLRETIVPSAKAYPLEAIMKDCRDY 104
           R +T+ST G+   +KR    +    LAVSL+A   ++R  I+P  + Y L  +++  R+ 
Sbjct: 283 RKVTVSTSGLVPQLKRFL-RESNCALAVSLNATTDEVRNWIMPINRKYKLSLLLETLREG 342

Query: 105 FLQTNR-RVSFEYALLAGVNDAVEHAVELAKLLHEWGGGYHVNLIPFNPVEGSEYQRPYK 164
               ++ +V FEY +LAGVND+++ A  L +L+   G    +NLI FNP  GS++ +  +
Sbjct: 343 LSSRHKYKVLFEYVMLAGVNDSMDDARRLVELVQ--GIPCKINLIQFNPHSGSQFIQTEE 402

Query: 165 KAVMAFVAALESHKITVSVRQTRGLDANAACGQL 198
             ++ F   L     TV +R +RG D  AACGQL
Sbjct: 403 DKMIKFRNVLAEGGCTVLMRFSRGNDQMAACGQL 433

[Close]

BLAST of HG10014909 vs. TAIR 10
Match: AT1G50050.1 (CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 protein) superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.7e-09
Identity = 29/41 (70.73%), Postives = 35/41 (85.37%), Query Frame = 0


Query: 171 AALESHKITVSVRQTRGLDANAACGQLRNQFQKNPLLLDSD 212
           AAL+S KIT SVRQTRGL A+AACGQLRN+FQK+P L  ++
Sbjct: 154 AALKSRKITASVRQTRGLGASAACGQLRNKFQKSPSLATAE 194

The following BLAST results are available for this feature:

BLAST of HG10014909 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892136.1	1.2e-96	97.31	probable dual-specificity RNA methyltransferase RlmN [Benincasa hispida]	[more]
KAA0050298.1	7.8e-96	93.26	putative dual-specificity RNA methyltransferase RlmN [Cucumis melo var. makuwa] ...	[more]
XP_022989725.1	5.1e-95	96.77	uncharacterized protein LOC111486721 [Cucurbita maxima]	[more]
XP_008461658.1	6.6e-95	95.70	PREDICTED: probable dual-specificity RNA methyltransferase RlmN [Cucumis melo]	[more]
KAG6601003.1	1.1e-94	96.24	hypothetical protein SDJN03_06236, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of HG10014909 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2JRQ8	3.9e-50	60.48	Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strai...	[more]
Q2JMN2	1.1e-49	58.24	Probable dual-specificity RNA methyltransferase RlmN OS=Synechococcus sp. (strai...	[more]
Q7NIV3	4.4e-49	61.11	Probable dual-specificity RNA methyltransferase RlmN OS=Gloeobacter violaceus (s...	[more]
B0C9F4	4.8e-48	59.01	Probable dual-specificity RNA methyltransferase RlmN OS=Acaryochloris marina (st...	[more]
B7K4N4	4.8e-48	56.82	Probable dual-specificity RNA methyltransferase RlmN OS=Rippkaea orientalis (str...	[more]

BLAST of HG10014909 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U4V1	3.8e-96	93.26	Putative dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa...	[more]
A0A6J1JN65	2.5e-95	96.77	uncharacterized protein LOC111486721 OS=Cucurbita maxima OX=3661 GN=LOC111486721...	[more]
A0A1S3CGI7	3.2e-95	95.70	probable dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1GZJ0	5.5e-95	96.24	uncharacterized protein LOC111458934 OS=Cucurbita moschata OX=3662 GN=LOC1114589...	[more]
A0A0A0KLM9	3.0e-93	95.63	Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G181470...	[more]

BLAST of HG10014909 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39670.1	3.0e-85	83.42	Radical SAM superfamily protein	[more]
AT2G39670.2	3.0e-85	83.42	Radical SAM superfamily protein	[more]
AT3G19630.1	9.4e-23	35.75	Radical SAM superfamily protein	[more]
AT1G60230.1	4.5e-17	35.71	Radical SAM superfamily protein	[more]
AT1G50050.1	2.7e-09	70.73	CAP (Cysteine-rich secretory proteins, Antigen 5, and Pathogenesis-related 1 pro...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 24..205 e-value: 2.4E-62 score: 212.6
None	No IPR available	PANTHER	PTHR30544:SF5	RADICAL SAM SUPERFAMILY PROTEIN	coord: 38..209
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 55..186
IPR040072	Methyltransferase (Class A)	PANTHER	PTHR30544	23S RRNA METHYLTRANSFERASE	coord: 38..209
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 1..189 score: 19.999535

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014909.1	HG10014909.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding