HG10014863 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014863
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cucumisin
Location	Chr02: 21076456 .. 21080263 (+)
RNA-Seq Expression	HG10014863
Synteny	HG10014863

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGGTTAATACTCCTTATTATTTACACTGTTAATTAAAAAACATCAGTCGCCATTCTAATATATTCACTCCAATTTGTTGTTTTGTATGTATTACAAAAAGTACTTTTATGCTTTTTCAGCGATTTTTCTCCAGAATCTTTGATATACACTTACAAGAGAAGTTTTAATGGATTTGCGGTGAAGCTTACCGACGAAGAAGCTCAAAGGATTGTTAGTACGTAATCGTTGTTAAAAAATACTTGCATGCATTCTGAACTATACCATTTGATTTTTTTTTTCAATAATTAATATCATGTACACTTTGTCATAAAACAAACTCTTCTTTCTTTCTTTTCTTTTCTTTTTTTTTGAAAAATATTTTTGACTTTTTTCTCTTGGATACATAGTTAAGGTGGGATGGAAAAAAATGGGTGTGTACCCAGCTAGAATGCACCAAAATATATTTTTTTATTCTTTTGTTTCAAATATCATTACAACCGCGTTTGTCATTATTGATTGTTTTATGACTTTTCAATACAACTAATTATAAGTCGTTGTTGACATTTATTATCTATCATTATAAAATTTTCTTTAACAATTTATCATTATTGTCAACAAATTTGTCACACCCCATCCCAAATTAACATATCTAACTTAGAGCATGACATGAAGACATGAAACTCATCATCAATAAAATCGTATAAACCTTAAATGAAGGAAAACCCATCAATGTATGTTTACTGAAAACGGATCACATTTGTATGTAAGAAATTTACTAATAAACCCTCCATAATAACCTATTAACAAACTTTAATATGCTCCTTTTTATAACATTTAAAACACACTATAGGAAATCTATAACATAATGTACAAATGTTGATCTAGAGAAGTGGCTATAGGTAAAGTTTTCAGTAAAAAATACGTTAATGGGTTTTCCTTCCTTTAAGTTTTACAGTTTTATTGACGATGAGTTTCATGTTTTCATGTCACACTCTAAGTTAGATAGGTTAATCTGGAGAGGGGCGTGATAAATTACTCAAAAGTTTGATTTTTTTTTATTATTATTTTTATTTTTTATTTATGAAACCTTTATATTGACATTGAGAATCTGTCATAAAAATGTTAATAATGTCAAGAATACCTTATATACGTCATAGGATTACATACAATGACACATATTTATGGTCATTGAGGTTCAACTAATAACAATGTTCCAGTGTCAAATAAATACATCTCATGAAATTTATTAAATGTCAAAGAAATGCTTAATTATAATGATAAAAATTTATTTGTTTGATACATGTCATTAAAATACATTTCCTCCAATTTGGACACAAAATACAAATATGAACTAACAATAGAGTTGGTAACATTAGCTCTTCAAAGTTGTTTATTCTTTTTTTTTTTTCAAATATTTTTGAGTTTTTTCAGTCACGTGCGTAGTTGGAGTCTACTGTAAAAAAAATAGGTGTAGTCTAGGATACATCAAGTTATTATTAATTAATCATATTTTTTGTTCATGATTTAGCAGTTCAAAGTTGTTTTATTGTTTCAAAATGTGTATTAAATCACCTGACGAAATGTCTCAGCTAAGGAGGGTGTGGTGTCTGTGTTTCCAAGTGAAAACAACCAACTTCACACGACAAGATCATGGGATTTTCTTGGTTTTCCATCAAATGTTGCTCGCATGAATAAGGTGGAGCAACGTAATCGTTGGAGTTTTCGACAGTGGAATTTGGTCGGACCATCCCAGTTTTAGTGACAAAGGCTACGGTCCTCCTCCAACCAAATGGAAGGGCATTTGCCAATTCTCTGCCAACTTTACTTGCAACAAGTAAACATTTATAAAACACTATATATCCTTATTTAATCTGCGATTTGCACTCAAAATTTTCAATAATTTTATTAATTATCATGAAAATATTTAGAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCAACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGCATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAGTTAACTCAGCCTTATTTTGAACATACCATTTCCATTGGAGCTTTCCATGCATTAAAACGTGGAATATTGACCTCCAGTAAATTGGGTCTCTAGGTTTCAAAACTCACACTTTTAACCTCAGATTTTCACCAAATACTCACTAACTCCAATCTTTAATTAGGGTTAATGTTTATTAATTAATTTAAAAGAATTCCAATCAATTAAGTTGTAGTATTTATTATCACTATTAAAATTAATTTTGTCACACAAGTGCAGCTTGGCAACAAAAATAACTTTCAGGTTGCTTTATTTTTTGCAAATGCATCCATCTTTCTACATCTAATATAATTAATCACACACAAAAGAAAAATTTTAAAATATTTTAAAAAAGAAGTAAAAAAAGTTTTTCATATAGCAAAATAGGATTGAAAAATTGGGTAGGATGCGCAAAAATAGATGTAATGTAGAATGCAAATAACTATTTTTTCGAGAAATATTTACATAATAATTATTTTATATAATTTTTTTAGGTTAAAAATATCGTTTTGGTTCCTATACTTTGAGATTTGTTCAATTTTAGTTTCTATATTTTCAAATGTCTTATATAGGTACCCTCAATAAATCTTAAATTTAGTTTCTATTGCTAGCTTATCGTTGACCTTTTTTTAATAAAAAAAAAATTAGTCCCTATTTTTTGTCTATTGTTGCATTTTTTCCTACAAATTTTGAAAATATATTCATATATTGTATTTTCTTATAAGAAAATTACTACTCTTATTTAACCAATTTTGATAAAAGTTAACTTCGGGAGACTAAATTTACCATTTATTGAAAGTGGGACACTTAAAAATATAAAACTAAAATTGAACAAACTCTAAAGTACAAGGACCAAAATGATATATTAACCATTTTTATATTAAAAAAATTCTAGTAATTTGTTTTAATACTAATAATAAGCTTGTTTTTACATTTTTTTTTTTATAGTTAATATGAAAGCTAGTTTAATGAATATAAGACACTATATTATTATCATCTCAAATGTTGATAGTTCAATCTTATATATGTTGAAACACATGCACAATTTGGGTTCTTATTATAAACTTGAAATGCTTTTTTATTCTAATTTTTTTATGAAAGTACAGAGGATTTAAGTTAACGCATTCGATGATCTGAAAAATCAATATCTCCTCATTTATGGTGGAGATGTACCAAACAAAGGATCCAATAGCTCCACCTCTAGGTAATCATTTTACTCTCTAATTTATATATGTAAAGATGAATTTGTAGGGTTAAAAAAAACTGAAGTCAATATTATTGAAAATGCAGATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

mRNA sequence

ATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCAACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGCATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

Coding sequence (CDS)

ATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCAACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGCATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

Protein sequence

MGSKLEDPDSAHLHHRAMLEEVVGRKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQLSSKKFASLGGPAGVLMQAYRDHAVSYPLPASTLKLEDGSKIKSYMTSTK

Homology

[Close]

BLAST of HG10014863 vs. NCBI nr
Match: XP_038891121.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 3.8e-70
Identity = 158/277 (57.04%), Postives = 168/277 (60.65%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           +KIIGAR YRS KTLPPGDIRSPIDT+GHGTHTASTVAGGL+ KASMNGLGL TARGGVP
Sbjct: 175 KKIIGARVYRSNKTLPPGDIRSPIDTDGHGTHTASTVAGGLMTKASMNGLGLGTARGGVP 234

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGK------------------ 144
           SARIAVYKVCWSDECADAD+LAAFDDAIADGVDIIS+S GGK                  
Sbjct: 235 SARIAVYKVCWSDECADADVLAAFDDAIADGVDIISLSVGGKLPKPYFQHTISIGAFHAL 294

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 295 KRGILTSNSAGNSGPDPYTTASLSPWLLSVAASTIDRKFVTQVQLGNKNNFQGISVNAFD 354

[Close]

BLAST of HG10014863 vs. NCBI nr
Match: XP_038891121.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 53.9 bits (128), Expect = 1.8e-03
Identity = 24/24 (100.00%), Postives = 24/24 (100.00%), Query Frame = 0


Query: 1  MGSKLEDPDSAHLHHRAMLEEVVG 25
          MGSKLEDPDSAHLHHRAMLEEVVG
Sbjct: 37 MGSKLEDPDSAHLHHRAMLEEVVG 60

HSP 2 Score: 204.1 bits (518), Expect = 1.1e-48
Identity = 127/277 (45.85%), Postives = 147/277 (53.07%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGARAYRS  TLPPGD+RSP DT+GHGTHTASTVAG LV++AS+ GLG+ TARGGVP
Sbjct: 179 RKIIGARAYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVP 238

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGK------------------ 144
            ARIAVYK+CWSD C+DAD+LAAFDDAIADGVDIIS+S GGK                  
Sbjct: 239 PARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAM 298

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 299 KRGILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFD 358

[Close]

BLAST of HG10014863 vs. NCBI nr
Match: BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-45
Identity = 119/274 (43.43%), Postives = 140/274 (51.09%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           KIIGARAYR   TLPPGD+ SP DT+GHGTHTASTVAGGLV++AS+ GLGL TARGGVPS
Sbjct: 181 KIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 240

Query: 86  ARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG-------------------- 145
           ARIA YK+CWSD C+DAD+LAAFDDAIADGV IIS+S GG                    
Sbjct: 241 ARIAAYKICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMK 300

Query: 146 ------------------------------------------------------------ 198
                                                                       
Sbjct: 301 HGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDM 360

[Close]

BLAST of HG10014863 vs. NCBI nr
Match: BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])

HSP 1 Score: 52.8 bits (125), Expect = 4.0e-03
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 0


Query: 1  MGSKLEDPDSAHLHHRAMLEEVVG 25
          MG+KLEDPDSAHLHHRAMLEEVVG
Sbjct: 42 MGNKLEDPDSAHLHHRAMLEEVVG 65

HSP 2 Score: 192.2 bits (487), Expect = 4.3e-45
Identity = 122/281 (43.42%), Postives = 139/281 (49.47%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           +KIIGARAYRS    PP DIRSP D++GHGTHTASTVAGGLVN+AS+ GL L TARGGVP
Sbjct: 176 KKIIGARAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVP 235

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
           SARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG                   
Sbjct: 236 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSM 295

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 296 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFD 355

[Close]

BLAST of HG10014863 vs. NCBI nr
Match: KAE8648003.1 (hypothetical protein Csa_021395 [Cucumis sativus])

HSP 1 Score: 191.4 bits (485), Expect = 7.3e-45
Identity = 121/281 (43.06%), Postives = 140/281 (49.82%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLVN+AS+ GL L TARGGVP
Sbjct: 189 RKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVP 248

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
           SARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG                   
Sbjct: 249 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSM 308

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 309 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQGYTINTFD 368

[Close]

BLAST of HG10014863 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 5.1e-41
Identity = 107/265 (40.38%), Postives = 129/265 (48.68%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGAR+Y   + + PGD+  P DTNGHGTHTAST AGGLV++A++ GLGL TARGGVP
Sbjct: 176 RKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVP 235

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
            ARIA YKVCW+D C+D D+LAA+DDAIADGVDIIS+S GG                   
Sbjct: 236 LARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAV 295

Query: 145 ------------------------------------------------------------ 187
                                                                       
Sbjct: 296 ERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD 355

HSP 2 Score: 50.8 bits (120), Expect = 2.0e-05
Identity = 22/24 (91.67%), Postives = 23/24 (95.83%), Query Frame = 0


Query: 1  MGSKLEDPDSAHLHHRAMLEEVVG 25
          MG KLEDPDSAHLHHRAMLE+VVG
Sbjct: 38 MGRKLEDPDSAHLHHRAMLEQVVG 61

[Close]

BLAST of HG10014863 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.4e-27
Identity = 63/108 (58.33%), Postives = 80/108 (74.07%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           KIIGA+ ++    +P G++RSPID +GHGTHT+STVAG LV  AS+ G+   TARG VPS
Sbjct: 183 KIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPS 242

Query: 86  ARIAVYKVCWS-DECADADMLAAFDDAIADGVDIISISAGGKFCKENS 133
           AR+A+YKVCW+   CAD D+LA F+ AI DGV+IISIS GG     +S
Sbjct: 243 ARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSS 290

[Close]

BLAST of HG10014863 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.9e-26
Identity = 61/101 (60.40%), Postives = 75/101 (74.26%), Query Frame = 0


Query: 26  KIIGARAYR-SKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 85
           K+IGA+ +    + LP G+  +  D +GHGTHT+ST+AG  V+ AS+ G+   TARGGVP
Sbjct: 182 KVIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVP 241

Query: 86  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG 126
           SARIA YKVCW   C D DMLAAFD+AI+DGVDIISIS GG
Sbjct: 242 SARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGG 282

[Close]

BLAST of HG10014863 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-23
Identity = 57/109 (52.29%), Postives = 73/109 (66.97%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDI---------RSPIDTNGHGTHTASTVAGGLVNKASMNGLG 84
           +KIIGAR Y        G +         RSP D++GHGTHTAST+AG +V+  S+ G+ 
Sbjct: 178 KKIIGARFYSKGLEAEIGPLENIVDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMA 237

Query: 85  LATARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAG 125
             TARGG PSAR+++YK CW   C+DAD+ AA DDAI DGVDI+S+S G
Sbjct: 238 KGTARGGAPSARLSIYKACWFGFCSDADVFAAMDDAIHDGVDILSLSLG 286

[Close]

BLAST of HG10014863 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.4e-22
Identity = 93/284 (32.75%), Postives = 110/284 (38.73%), Query Frame = 0


Query: 19  LEEVVGRKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLAT 78
           L+     K+IGAR Y            S  D  GHGTHTAST AG  V  AS  GL   T
Sbjct: 169 LKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 228

Query: 79  ARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISIS---------------- 138
           ARGGVPSARIA YKVC+ + C D D+LAAFDDAIADGVD+ISIS                
Sbjct: 229 ARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAI 288

Query: 139 ------------------------------------------------------------ 198
                                                                       
Sbjct: 289 GSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGI 348

[Close]

BLAST of HG10014863 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 5.3e-49
Identity = 127/277 (45.85%), Postives = 147/277 (53.07%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGARAYRS  TLPPGD+RSP DT+GHGTHTASTVAG LV++AS+ GLG+ TARGGVP
Sbjct: 179 RKIIGARAYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVP 238

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGK------------------ 144
            ARIAVYK+CWSD C+DAD+LAAFDDAIADGVDIIS+S GGK                  
Sbjct: 239 PARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAM 298

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 299 KRGILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFD 358

[Close]

BLAST of HG10014863 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 7.1e-46
Identity = 119/274 (43.43%), Postives = 140/274 (51.09%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           KIIGARAYR   TLPPGD+ SP DT+GHGTHTASTVAGGLV++AS+ GLGL TARGGVPS
Sbjct: 181 KIIGARAYRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPS 240

Query: 86  ARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG-------------------- 145
           ARIA YK+CWSD C+DAD+LAAFDDAIADGV IIS+S GG                    
Sbjct: 241 ARIAAYKICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMK 300

Query: 146 ------------------------------------------------------------ 198
                                                                       
Sbjct: 301 HGILTSNSAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDM 360

[Close]

BLAST of HG10014863 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.0e-03
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 0


Query: 1  MGSKLEDPDSAHLHHRAMLEEVVG 25
          MG+KLEDPDSAHLHHRAMLEEVVG
Sbjct: 42 MGNKLEDPDSAHLHHRAMLEEVVG 65

HSP 2 Score: 192.2 bits (487), Expect = 2.1e-45
Identity = 122/281 (43.42%), Postives = 139/281 (49.47%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           +KIIGARAYRS    PP DIRSP D++GHGTHTASTVAGGLVN+AS+ GL L TARGGVP
Sbjct: 176 KKIIGARAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVP 235

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
           SARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG                   
Sbjct: 236 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSM 295

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 296 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFD 355

[Close]

BLAST of HG10014863 vs. ExPASy TrEMBL
Match: A0A0A0KLR4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 3.5e-45
Identity = 121/281 (43.06%), Postives = 140/281 (49.82%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLVN+AS+ GL L TARGGVP
Sbjct: 139 RKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVP 198

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
           SARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG                   
Sbjct: 199 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSM 258

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 259 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQGYTINTFD 318

[Close]

BLAST of HG10014863 vs. ExPASy TrEMBL
Match: A0A5A7UBK2 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001150 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 6.0e-45
Identity = 121/281 (43.06%), Postives = 140/281 (49.82%), Query Frame = 0


Query: 25  RKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 84
           RKIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLVN+AS+ GL   TARGGVP
Sbjct: 177 RKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVP 236

Query: 85  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG------------------- 144
           SARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG                   
Sbjct: 237 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSM 296

Query: 145 ------------------------------------------------------------ 199
                                                                       
Sbjct: 297 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFD 356

[Close]

BLAST of HG10014863 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 122.5 bits (306), Expect = 3.9e-28
Identity = 63/108 (58.33%), Postives = 80/108 (74.07%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           KIIGA+ ++    +P G++RSPID +GHGTHT+STVAG LV  AS+ G+   TARG VPS
Sbjct: 183 KIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPS 242

Query: 86  ARIAVYKVCWS-DECADADMLAAFDDAIADGVDIISISAGGKFCKENS 133
           AR+A+YKVCW+   CAD D+LA F+ AI DGV+IISIS GG     +S
Sbjct: 243 ARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSS 290

[Close]

BLAST of HG10014863 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 5.6e-27
Identity = 61/101 (60.40%), Postives = 75/101 (74.26%), Query Frame = 0


Query: 26  KIIGARAYR-SKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 85
           K+IGA+ +    + LP G+  +  D +GHGTHT+ST+AG  V+ AS+ G+   TARGGVP
Sbjct: 182 KVIGAKYFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVP 241

Query: 86  SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG 126
           SARIA YKVCW   C D DMLAAFD+AI+DGVDIISIS GG
Sbjct: 242 SARIAAYKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGG 282

[Close]

BLAST of HG10014863 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-23
Identity = 93/284 (32.75%), Postives = 110/284 (38.73%), Query Frame = 0


Query: 19  LEEVVGRKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLAT 78
           L+     K+IGAR Y            S  D  GHGTHTAST AG  V  AS  GL   T
Sbjct: 133 LKFACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 192

Query: 79  ARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISIS---------------- 138
           ARGGVPSARIA YKVC+ + C D D+LAAFDDAIADGVD+ISIS                
Sbjct: 193 ARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAI 252

Query: 139 ------------------------------------------------------------ 198
                                                                       
Sbjct: 253 GSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGI 312

[Close]

BLAST of HG10014863 vs. TAIR 10
Match: AT5G58820.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 105.1 bits (261), Expect = 6.4e-23
Identity = 58/102 (56.86%), Postives = 68/102 (66.67%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           K+IGAR Y S+ T          D  GHGTHTAST AG  V  AS  G+G  TARGGVP+
Sbjct: 176 KLIGARDYTSEGTR---------DLQGHGTHTASTAAGNAVADASFFGIGNGTARGGVPA 235

Query: 86  ARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKF 128
           +RIA YKVC   +C  A +L+AFDDAIADGVD+ISIS   +F
Sbjct: 236 SRIAAYKVCSEKDCTAASLLSAFDDAIADGVDLISISLASEF 268

[Close]

BLAST of HG10014863 vs. TAIR 10
Match: AT5G58830.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 55/102 (53.92%), Postives = 68/102 (66.67%), Query Frame = 0


Query: 26  KIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPS 85
           K+IGAR Y S+ T          D  GHGTHT ST AG  V   S  G+G  TARGGVP+
Sbjct: 171 KLIGARDYTSEGTR---------DLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPA 230

Query: 86  ARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKF 128
           +R+A YKVC    C+D ++L+AFDDAIADGVD+IS+S GG +
Sbjct: 231 SRVAAYKVCTITGCSDDNVLSAFDDAIADGVDLISVSLGGDY 263

The following BLAST results are available for this feature:

BLAST of HG10014863 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891121.1	3.8e-70	57.04	cucumisin-like [Benincasa hispida]	[more]
XP_038891121.1	1.8e-03	100.00	cucumisin-like [Benincasa hispida]	[more]
BBK45496.1	1.5e-45	43.43	pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 tri...	[more]
BBK45496.1	4.0e-03	95.83	pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 tri...	[more]
KAE8648003.1	7.3e-45	43.06	hypothetical protein Csa_021395 [Cucumis sativus]	[more]

BLAST of HG10014863 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	5.1e-41	40.38	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9LLL8	5.4e-27	58.33	Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2...	[more]
Q9LZS6	7.9e-26	60.40	Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3...	[more]
A9JQS7	1.6e-23	52.29	Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1	[more]
Q9FIF8	2.4e-22	32.75	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S...	[more]

BLAST of HG10014863 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NBW1	5.3e-49	45.85	Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A4P2YW59	7.1e-46	43.43	Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2...	[more]
A0A4P2YW59	2.0e-03	95.83	Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2...	[more]
A0A0A0KLR4	3.5e-45	43.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=3 SV=1	[more]
A0A5A7UBK2	6.0e-45	43.06	Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00115...	[more]

BLAST of HG10014863 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G00230.1	3.9e-28	58.33	xylem serine peptidase 1	[more]
AT5G03620.1	5.6e-27	60.40	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59190.1	1.7e-23	32.75	subtilase family protein	[more]
AT5G58820.1	6.4e-23	56.86	Subtilisin-like serine endopeptidase family protein	[more]
AT5G58830.1	2.4e-22	53.92	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 24..126 e-value: 1.2E-43 score: 151.6
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 31..127
None	No IPR available	GENE3D	3.50.30.30		coord: 127..198 e-value: 1.5E-8 score: 35.9
None	No IPR available	PANTHER	PTHR10795:SF348	SUBTILISIN-LIKE SERINE PROTEASE	coord: 25..125
None	No IPR available	PANTHER	PTHR10795:SF348	SUBTILISIN-LIKE SERINE PROTEASE	coord: 125..196
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 123..198 e-value: 5.57673E-9 score: 50.4899
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 43..145 e-value: 2.4E-15 score: 56.6
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 25..125
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 125..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014863.1	HG10014863.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity