HG10014859 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014859 vs. NCBI nr
Match: XP_038892403.1 (cucumisin-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 573/721 (79.47%), Postives = 623/721 (86.41%), Query Frame = 0

Query: 5   LIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAPESVV 64
           LIFKL FL++FFSTLLASSLDS DDDKKIY+VYMGRK+KDD DSAH H SSFPFAPESVV
Sbjct: 11  LIFKLFFLSLFFSTLLASSLDS-DDDKKIYIVYMGRKIKDDPDSAHLHHSSFPFAPESVV 70

Query: 65  YTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPRVKQV 124
           Y YKRSFNGFAVKLTKEEAEKIA MEGVVSVFPNK+N+LHTTRSWDF+ FPKNVPRVKQV
Sbjct: 71  YMYKRSFNGFAVKLTKEEAEKIASMEGVVSVFPNKINKLHTTRSWDFMNFPKNVPRVKQV 130

Query: 125 ENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIGRSLP 184
           E+N++VGV D+GIWPESPSF+DK FGP PSKWKG+C   NFTCNRKIIGARAY IGR LP
Sbjct: 131 ESNIVVGVFDTGIWPESPSFNDKGFGPPPSKWKGTCLVFNFTCNRKIIGARAYHIGRPLP 190

Query: 185 HGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCS 244
            G+V+SPRDTNGHGTHTASTAAGGL+S+AS YGLGLGT RGGVPSARIA YKICW+D CS
Sbjct: 191 RGEVESPRDTNGHGTHTASTAAGGLVSKASLYGLGLGTARGGVPSARIAAYKICWSDSCS 250

Query: 245 DIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPK 304
           D+D+LAAFDDAIADGVDIISLSVG   SR YF D IAIGSFHA Q GILTSNSAGN GP+
Sbjct: 251 DLDILAAFDDAIADGVDIISLSVGGNESRQYFRDPIAIGSFHAMQKGILTSNSAGNDGPR 310

Query: 305 LFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYDVLKT 364
            FTTTSLSPWLLSVAAST DRKF+TKVQIGNKNSF+GVSINTFD  GQYPLVAG D+   
Sbjct: 311 YFTTTSLSPWLLSVAASTTDRKFVTKVQIGNKNSFQGVSINTFDTKGQYPLVAGRDIPNK 370

Query: 365 SFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSD--TRDTAFSN 424
            FH+STSR C NNSV+PKLVKGKI+ C+  +   +F + GG  GVL   D  T D  FS 
Sbjct: 371 GFHNSTSRYCFNNSVDPKLVKGKIVFCETNVDSSEFISLGGAMGVLGKGDKNTMDCEFSY 430

Query: 425 PLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVK 484
           PLPSSTLD +DA  I HYI + R PTATIFKS   HN  +P + SFSSRGPNA TKDL+K
Sbjct: 431 PLPSSTLDVDDATIISHYIDNTRFPTATIFKSTATHNAPSPVVVSFSSRGPNAATKDLIK 490

Query: 485 PDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAA 544
           PDLSAPGVEILAAWPPVAPVGG  R TLYNIISGTSMSCPH+TGIA YVKTFNPTWSPAA
Sbjct: 491 PDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYVKTFNPTWSPAA 550

Query: 545 IKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTN 604
           IKSALMTTASPMN++F+P+AEFAYGSGHVN LKA+ PGLVYDANE+DYVKFLCGQGYTT+
Sbjct: 551 IKSALMTTASPMNSQFNPQAEFAYGSGHVNPLKALTPGLVYDANETDYVKFLCGQGYTTD 610

Query: 605 IVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYRATI 664
           +VRIITNDNS C S N GRVWDLNYPSFGL VSHSK F+QYFTRTLTSVAS ASTY+A I
Sbjct: 611 LVRIITNDNSVCISTNTGRVWDLNYPSFGLSVSHSKTFNQYFTRTLTSVASGASTYKAMI 670

Query: 665 STPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPITITS 724
           S P GL+I V P VLSFNG GD KSFKLTVRGTMR+ IVSASL+WSD VHTVRSPITITS
Sbjct: 671 SAPKGLAITVKPKVLSFNGTGDMKSFKLTVRGTMRESIVSASLIWSDSVHTVRSPITITS 730

BLAST of HG10014859 vs. NCBI nr
Match: XP_008461722.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 552/724 (76.24%), Postives = 613/724 (84.67%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SLI KLVF N+FF TLLASSLDS  DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 7   MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 66

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN  HTTRSWDF+GF +NVPR
Sbjct: 67  ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 126

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 127 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 186

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW 
Sbjct: 187 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 246

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D CSD D+LAAFDDAIADGVDIISLSVG   +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 247 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 306

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
           +GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD  GQYPLV G  
Sbjct: 307 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 366

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTV-SDTRDTA 420
           V  T F  + S  C NNSV+ KLVKGKILIC+       F   GG AGVL + ++  D A
Sbjct: 367 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 426

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S P+PS+ LD NDA   + YI+S  SPTATIFKS    N  AP + SFSSRGPN ITK+
Sbjct: 427 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 486

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 487 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 546

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + +AEFAYG+GHVN LKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 547 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 606

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 607 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 666

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 667 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 726

Query: 721 ITSL 724
           +TSL
Sbjct: 727 VTSL 728

BLAST of HG10014859 vs. NCBI nr
Match: KAA0051615.1 (cucumisin-like [Cucumis melo var. makuwa])

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 552/724 (76.24%), Postives = 613/724 (84.67%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SLI KLVF N+FF TLLASSLDS  DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1   MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN  HTTRSWDF+GF +NVPR
Sbjct: 61  ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW 
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D CSD D+LAAFDDAIADGVDIISLSVG   +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
           +GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD  GQYPLV G  
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 360

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTV-SDTRDTA 420
           V  T F  + S  C NNSV+ KLVKGKILIC+       F   GG AGVL + ++  D A
Sbjct: 361 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S P+PS+ LD NDA   + YI+S  SPTATIFKS    N  AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + +AEFAYG+GHVN LKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 720

Query: 721 ITSL 724
           +TSL
Sbjct: 721 VTSL 722

BLAST of HG10014859 vs. NCBI nr
Match: XP_038892506.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 551/732 (75.27%), Postives = 612/732 (83.61%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSF---- 60
           MSSSL+FKL F++ FFST+L SS +S DD KKIY+VYMGRKL +D DSAH H  +     
Sbjct: 7   MSSSLVFKLFFVSFFFSTILTSSFESDDDGKKIYIVYMGRKL-EDPDSAHLHHRAMLEQV 66

Query: 61  ---PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLG 120
               FAPESV+Y+YKRSFNGF VKLT+EEAEKIA MEGVVSVF N+MN LHTTRSWDFL 
Sbjct: 67  VGSNFAPESVLYSYKRSFNGFVVKLTEEEAEKIASMEGVVSVFLNEMNVLHTTRSWDFLN 126

Query: 121 FPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIG 180
           FP+N+ RV QVE+N++VGVLDSGIWPESPSF+DK F   PSKWKGSCQA NFTCNRKIIG
Sbjct: 127 FPQNIQRVNQVESNIVVGVLDSGIWPESPSFNDKGFDSPPSKWKGSCQAFNFTCNRKIIG 186

Query: 181 ARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIA 240
            RAY IG  L  GDV+SPRDT+GHGTHTASTAAGGL+SQA+ Y LGLGT RGGVP ARIA
Sbjct: 187 GRAYHIGGPLRPGDVNSPRDTDGHGTHTASTAAGGLVSQANLYRLGLGTARGGVPLARIA 246

Query: 241 VYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGIL 300
           VYKICW DGCSD D+LAA+DDAIADGVDIISLSVG    R YF D IAIGSFHA + GIL
Sbjct: 247 VYKICWKDGCSDADILAAYDDAIADGVDIISLSVGANKPRQYFSDPIAIGSFHAIEKGIL 306

Query: 301 TSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQY 360
           TSNSAGN GP  FTTTSLSPWLLSVAAST+DRKF+T+VQIGN  SF+GVSINTF+MNGQY
Sbjct: 307 TSNSAGNEGPNFFTTTSLSPWLLSVAASTIDRKFVTQVQIGNGQSFQGVSINTFEMNGQY 366

Query: 361 PLVAGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGTAGVLTV 420
           PLV G D+    F  STSR C N SVNP L++GKI++C+      +FF +  G AGVL +
Sbjct: 367 PLVLGRDIPNIGFDSSTSRYCFNKSVNPYLLRGKIVLCEASFGPAEFFKSLDGAAGVLML 426

Query: 421 SDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRG 480
           + TRD A S P PSSTLDPNDA  IF YI+S   PTATIFKS  + N SAP + SFSSRG
Sbjct: 427 ASTRDHASSYPFPSSTLDPNDATDIFRYIYSTSFPTATIFKSTAILNTSAPVVVSFSSRG 486

Query: 481 PNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVK 540
           PNA TKDL+KPDLSAPGVEILAAWPPVAPVGG  R TLYNIISGTSMSCPH+TGIA YVK
Sbjct: 487 PNAATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYVK 546

Query: 541 TFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVK 600
           TFNPTWSPAAIKSALMTTASPMNA+F P+ EFAYGSGHVN LKAVRPGLVYDANESDYVK
Sbjct: 547 TFNPTWSPAAIKSALMTTASPMNARFHPQGEFAYGSGHVNPLKAVRPGLVYDANESDYVK 606

Query: 601 FLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVA 660
           FLCGQGY+T++VR IT D SACTS NIGRVWDLNYPSFGL VS S+ F QYFTRTLTSVA
Sbjct: 607 FLCGQGYSTSMVRRITGDYSACTSGNIGRVWDLNYPSFGLSVSGSQTFSQYFTRTLTSVA 666

Query: 661 SQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVH 720
           SQASTYRA IS P GL+I VNPNVLSFNGIGD+KSF LT+RG++ +Y+VSASLVW+DGVH
Sbjct: 667 SQASTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTLTIRGSVNQYVVSASLVWTDGVH 726

Query: 721 TVRSPITITSLS 725
           TVRSPIT+T+LS
Sbjct: 727 TVRSPITVTTLS 737

BLAST of HG10014859 vs. NCBI nr
Match: XP_038892404.1 (cucumisin-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1072.0 bits (2771), Expect = 2.2e-309
Identity = 530/669 (79.22%), Postives = 576/669 (86.10%), Query Frame = 0

Query: 57  PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPK 116
           PFAPESVVY YKRSFNGFAVKLTKEEAEKIA MEGVVSVFPNK+N+LHTTRSWDF+ FPK
Sbjct: 5   PFAPESVVYMYKRSFNGFAVKLTKEEAEKIASMEGVVSVFPNKINKLHTTRSWDFMNFPK 64

Query: 117 NVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARA 176
           NVPRVKQVE+N++VGV D+GIWPESPSF+DK FGP PSKWKG+C   NFTCNRKIIGARA
Sbjct: 65  NVPRVKQVESNIVVGVFDTGIWPESPSFNDKGFGPPPSKWKGTCLVFNFTCNRKIIGARA 124

Query: 177 YRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYK 236
           Y IGR LP G+V+SPRDTNGHGTHTASTAAGGL+S+AS YGLGLGT RGGVPSARIA YK
Sbjct: 125 YHIGRPLPRGEVESPRDTNGHGTHTASTAAGGLVSKASLYGLGLGTARGGVPSARIAAYK 184

Query: 237 ICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSN 296
           ICW+D CSD+D+LAAFDDAIADGVDIISLSVG   SR YF D IAIGSFHA Q GILTSN
Sbjct: 185 ICWSDSCSDLDILAAFDDAIADGVDIISLSVGGNESRQYFRDPIAIGSFHAMQKGILTSN 244

Query: 297 SAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLV 356
           SAGN GP+ FTTTSLSPWLLSVAAST DRKF+TKVQIGNKNSF+GVSINTFD  GQYPLV
Sbjct: 245 SAGNDGPRYFTTTSLSPWLLSVAASTTDRKFVTKVQIGNKNSFQGVSINTFDTKGQYPLV 304

Query: 357 AGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSD-- 416
           AG D+    FH+STSR C NNSV+PKLVKGKI+ C+  +   +F + GG  GVL   D  
Sbjct: 305 AGRDIPNKGFHNSTSRYCFNNSVDPKLVKGKIVFCETNVDSSEFISLGGAMGVLGKGDKN 364

Query: 417 TRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPN 476
           T D  FS PLPSSTLD +DA  I HYI + R PTATIFKS   HN  +P + SFSSRGPN
Sbjct: 365 TMDCEFSYPLPSSTLDVDDATIISHYIDNTRFPTATIFKSTATHNAPSPVVVSFSSRGPN 424

Query: 477 AITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTF 536
           A TKDL+KPDLSAPGVEILAAWPPVAPVGG  R TLYNIISGTSMSCPH+TGIA YVKTF
Sbjct: 425 AATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYVKTF 484

Query: 537 NPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFL 596
           NPTWSPAAIKSALMTTASPMN++F+P+AEFAYGSGHVN LKA+ PGLVYDANE+DYVKFL
Sbjct: 485 NPTWSPAAIKSALMTTASPMNSQFNPQAEFAYGSGHVNPLKALTPGLVYDANETDYVKFL 544

Query: 597 CGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQ 656
           CGQGYTT++VRIITNDNS C S N GRVWDLNYPSFGL VSHSK F+QYFTRTLTSVAS 
Sbjct: 545 CGQGYTTDLVRIITNDNSVCISTNTGRVWDLNYPSFGLSVSHSKTFNQYFTRTLTSVASG 604

Query: 657 ASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTV 716
           ASTY+A IS P GL+I V P VLSFNG GD KSFKLTVRGTMR+ IVSASL+WSD VHTV
Sbjct: 605 ASTYKAMISAPKGLAITVKPKVLSFNGTGDMKSFKLTVRGTMRESIVSASLIWSDSVHTV 664

Query: 717 RSPITITSL 724
           RSPITITSL
Sbjct: 665 RSPITITSL 673

BLAST of HG10014859 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 6.1e-310
Identity = 541/732 (73.91%), Postives = 603/732 (82.38%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSF---- 60
           MSSSLIFKL F ++FFS  LAS LDS DD K IY+VYMGRKL +D DSAH H  +     
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60

Query: 61  ---PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLG 120
               FAPESV++TYKRSFNGFAVKLT+EEAEKIA MEGVVSVF N+MN+LHTTRSWDFLG
Sbjct: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120

Query: 121 FPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKII 180
           FP  VPR  QVE+N++VGVLD+GIWPESPSFDD+ F P P KWKG+C+ + NF CNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180

Query: 181 GARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARI 240
           GAR+Y IGR +  GDV+ PRDTNGHGTHTASTAAGGL+SQA+ YGLGLGT RGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240

Query: 241 AVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGI 300
           A YK+CWNDGCSD D+LAA+DDAIADGVDIISLSVG    R YF+D+IAIGSFHA + GI
Sbjct: 241 AAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300

Query: 301 LTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ 360
           LTSNSAGN GP  FTT SLSPWLLSVAASTMDRKF+T+VQIGN  SF+GVSINTFD N  
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQY 360

Query: 361 YPLVAGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGTAGVLT 420
           YPLV+G D+  T F  STSR C + SVNP L+KGKI++C+      +FF +  G AGVL 
Sbjct: 361 YPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420

Query: 421 VSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSR 480
            S+TRD A S PLPSS LDPND      YI+S RSP ATIFKS T+ N SAP + SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480

Query: 481 GPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYV 540
           GPN  TKD++KPD+S PGVEILAAWP VAPVGG  R TL+NIISGTSMSCPHITGIA YV
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYV 540

Query: 541 KTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYV 600
           KT+NPTWSPAAIKSALMTTASPMNA+F+P+AEFAYGSGHVN LKAVRPGLVYDANESDYV
Sbjct: 541 KTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYV 600

Query: 601 KFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSV 660
           KFLCGQGY T  VR IT D SACTS N GRVWDLNYPSFGL VS S+ F+QYF RTLTSV
Sbjct: 601 KFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSV 660

Query: 661 ASQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGV 720
           A QASTYRA IS P GL+I VNPNVLSFNG+GDRKSF LTVRG+++ ++VSASLVWSDGV
Sbjct: 661 APQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 720

Query: 721 HTVRSPITITSL 724
           H VRSPITITSL
Sbjct: 721 HYVRSPITITSL 730

BLAST of HG10014859 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.8e-179
Identity = 348/721 (48.27%), Postives = 466/721 (64.63%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           LL+S     D+D ++Y+VYMG    + D      H S       ES     +V +YKRSF
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 138
           NGFA +LT+ E   IA++EGVVSVFPNK+ QLHTT SWDF+G    KN  R   +E++ I
Sbjct: 78  NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+D+GIWPES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RDT+GHGTHTASTAAG  +   SF+G+G GTVRGGVP++RIA YK+C + GCS   L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L++FDDAIADGVD+I++S+GF+    +  D IAIG+FHA   GILT +SAGNSGPK  T 
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 378
           + ++PW+ +VAAST +R FITKV +GN  +  G S+N FDM G +YPLV G     ++  
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             T+  C    +N   VKGKIL+C     Y    + G  A ++  S   D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHLPAS 437

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D  ++  YI S  SP A + K+ T+ N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R+  Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557

Query: 559 LMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 618
           +MTTA P+ AK       EFAYG+GHV+ + A+ PGLVY+ +++D++ FLCG  YT+  +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617

Query: 619 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 678
           +II+ D   C+ KN     +LNYPS    +S +   F   F RTLT+V +  STY++ + 
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677

Query: 679 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 721
             +G  LSIKV P+VL F  + +++SF +TV G+     V  SA+L+WSDG H VRSPI 
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 728

BLAST of HG10014859 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 2.8e-177
Identity = 338/718 (47.08%), Postives = 472/718 (65.74%), Query Frame = 0

Query: 26  SADDDKK---IYVVYMGRKLKDDRDSAHSHQSSF-------PFAPESVVYTYKRSFNGFA 85
           SA+D ++   +Y+VYMG  L + + S  SH  S          A   +V +YKRSFNGFA
Sbjct: 22  SANDYRQASSVYIVYMG-TLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFA 81

Query: 86  VKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPRVKQVENNVIVGVLDS 145
             L++ E++K+ +M+ VVSVFP+K ++L TTRSWDF+GF +   R    E++VIVGV+DS
Sbjct: 82  ANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDS 141

Query: 146 GIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDVDSPRDT 205
           GIWPES SFDD+ FGP P KWKGSC+  + F CN K+IGAR Y       +   DS RD 
Sbjct: 142 GIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY-------NKFADSARDE 201

Query: 206 NGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDLLAAFDD 265
            GHGTHTASTAAG  +  ASFYGL  GT RGGVPSARIA YK+C+N  C+D+D+LAAFDD
Sbjct: 202 EGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDD 261

Query: 266 AIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTTTSLSPW 325
           AIADGVD+IS+S+  +        S+AIGSFHA   GI+T+ SAGN+GP   +  ++SPW
Sbjct: 262 AIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPW 321

Query: 326 LLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFHDSTSRS 385
           +++VAAS  DR+FI +V +GN  +  G+S+NTF++NG ++P+V G +V + +   + +  
Sbjct: 322 MITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGY 381

Query: 386 CRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSSTLDPND 445
           C +  V+ +LVKGKI++C + L Y + +  G    ++  +   D+AF  P P+S+L   D
Sbjct: 382 CSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFED 441

Query: 446 ADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSAPGVEIL 505
             +I  YI SA  P A I ++  + +  AP++ SFSSRGP+ + ++L+KPD+SAPG+EIL
Sbjct: 442 YKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEIL 501

Query: 506 AAWPPVAPVGG-----SHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSALM 565
           AA+ PVA           R   Y+++SGTSM+CPH+ G+A YVK+F+P WSP+AIKSA+M
Sbjct: 502 AAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIM 561

Query: 566 TTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIVRIIT 625
           TTA+PMN K +PE EFAYGSG +N  KA  PGLVY+    DY+K LC +G+ +  +   +
Sbjct: 562 TTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTS 621

Query: 626 NDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYRAT-ISTPNG 685
             N  C+ +    V DLNYP+   FVS    F+  F RT+T+V    STY+A+ +     
Sbjct: 622 GQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPE 681

Query: 686 LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRK--YIVSASLVWSDGVHTVRSPITITSL 724
           L I + P +L F  + ++KSF +T+ G   K    VS+S+VWSDG H+VRSPI   S+
Sbjct: 682 LQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of HG10014859 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.5e-175
Identity = 337/721 (46.74%), Postives = 462/721 (64.08%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           L  SS+ +  DDK++Y+VYMG    + D      H +       ES     +V +YKRSF
Sbjct: 17  LFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSF 76

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNV--PRVKQVENNVI 138
           NGFA +LT+ E E++A M GVVSVFPNK  QL TT SWDF+G  + +   R   VE++ I
Sbjct: 77  NGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTI 136

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+DSGI PES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 137 IGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY---------TS 196

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RD +GHGTHTASTAAG  +  ASF+G+G GTVRGGVP++R+A YK+C   GCS   L
Sbjct: 197 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 256

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L+AFDDAIADGVD+I++S+G +T+  +  D IAIG+FHA   G+LT NSAGNSGPK  + 
Sbjct: 257 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 316

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ-YPLVAGYDVLKTSFH 378
           + ++PW+L+VAAST +R F+TKV +GN  +  G S+N ++M G+ YPLV G     ++  
Sbjct: 317 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 376

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             ++  C  + V+   VKGKIL+C             G  G++  +   D AF +PLP++
Sbjct: 377 AESAGLCELSCVDKSRVKGKILVCGGPGGL-KIVESVGAVGLIYRTPKPDVAFIHPLPAA 436

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D +++  Y+ S  SP A + K+  + N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 437 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 496

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R   Y+++SGTSMSCPH+ G+A YVKTFNP WSP+ I+SA
Sbjct: 497 PGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSA 556

Query: 559 LMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 618
           +MTTA P+NA        EFAYGSGHV+ + A  PGLVY+ ++SD++ FLCG  YT+ ++
Sbjct: 557 IMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVL 616

Query: 619 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 678
           ++I+ +   C+        +LNYPS    +S S   F   F RTLT+V +  STY + + 
Sbjct: 617 KVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVV 676

Query: 679 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 721
             +G  L +K+ P+VLSF  + +++SF +TV G+     V  SA+L+WSDG H VRSPI 
Sbjct: 677 AGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIV 727

BLAST of HG10014859 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 2.0e-175
Identity = 341/748 (45.59%), Postives = 482/748 (64.44%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSAD-DDKKIYVVYMGR-KLKDDRDSAHSHQSSF-- 60
           M+    F  +F ++   +L + S D  D  D+++Y+VY+G    +++      H S    
Sbjct: 1   MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60

Query: 61  ----PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFL 120
                     +V +YK+SFNGFA +LT+ E +++A ME VVSVFP++  +L TT SW+F+
Sbjct: 61  ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120

Query: 121 GFPKNV--PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNR 180
           G  + +   R + +E++ I+GV+DSGI+PES SF D+ FGP P KWKG+C    NFTCN 
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180

Query: 181 KIIGARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPS 240
           K+IGAR Y   +S  +    + RD +GHGTHTAS AAG  ++ ++FYGLG GT RGGVP+
Sbjct: 181 KVIGARDY-TAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240

Query: 241 ARIAVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQ 300
           ARIAVYK+C N+GC    +++AFDDAIADGVD+IS+S+  +   P+  D IAIG+FHA  
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300

Query: 301 NGILTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDM 360
            G+LT N+AGN+GPK+ T TS +PW+ SVAAS  +R F+ KV +G+     G S+NT+DM
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360

Query: 361 NG-QYPLVAGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAG 420
           NG  YPLV G     ++     +R C    ++ KLVKGKI++C       +     G  G
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL-GAVG 420

Query: 421 VLTVSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASF 480
            +  +   D AF    P S L  +D  ++  Y++S ++P AT+ KS  + N  AP +ASF
Sbjct: 421 SIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 480

Query: 481 SSRGPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSH---RKTLYNIISGTSMSCPHIT 540
           SSRGP++I  D++KPD++APGVEILAA+ P +    S    R+  Y+++SGTSM+CPH+ 
Sbjct: 481 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 540

Query: 541 GIAVYVKTFNPTWSPAAIKSALMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVY 600
           G+A YVKTF+P WSP+ I+SA+MTTA PMNA        EFAYGSGHV+ + A+ PGLVY
Sbjct: 541 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 600

Query: 601 DANESDYVKFLCGQGYTTNIVRIITNDNSACT---SKNIGRVWDLNYPSFGLFVSHSKIF 660
           +  ++D++ FLCG  YT++ +RII+ DNS CT   SK + R  +LNYP+    VS +K F
Sbjct: 601 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPF 660

Query: 661 DQYFTRTLTSVASQASTYRATISTPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTM-- 720
           +  F RT+T+V  Q STY A +    G  LSIKV+P VLS   + +++SF +TV      
Sbjct: 661 NITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIG 720

Query: 721 RKYIVSASLVWSDGVHTVRSPITITSLS 725
            K  VSA+L+WSDG H VRSPI + ++S
Sbjct: 721 TKQPVSANLIWSDGTHNVRSPIIVYAMS 741

BLAST of HG10014859 vs. ExPASy TrEMBL
Match: A0A5A7UD73 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001640 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 552/724 (76.24%), Postives = 613/724 (84.67%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SLI KLVF N+FF TLLASSLDS  DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1   MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN  HTTRSWDF+GF +NVPR
Sbjct: 61  ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW 
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D CSD D+LAAFDDAIADGVDIISLSVG   +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
           +GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD  GQYPLV G  
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 360

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTV-SDTRDTA 420
           V  T F  + S  C NNSV+ KLVKGKILIC+       F   GG AGVL + ++  D A
Sbjct: 361 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S P+PS+ LD NDA   + YI+S  SPTATIFKS    N  AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + +AEFAYG+GHVN LKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 720

Query: 721 ITSL 724
           +TSL
Sbjct: 721 VTSL 722

BLAST of HG10014859 vs. ExPASy TrEMBL
Match: A0A1S3CFD6 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 552/724 (76.24%), Postives = 613/724 (84.67%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SLI KLVF N+FF TLLASSLDS  DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 7   MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 66

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN  HTTRSWDF+GF +NVPR
Sbjct: 67  ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 126

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 127 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 186

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW 
Sbjct: 187 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 246

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D CSD D+LAAFDDAIADGVDIISLSVG   +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 247 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 306

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
           +GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+G SINTFD  GQYPLV G  
Sbjct: 307 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGRQ 366

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTV-SDTRDTA 420
           V  T F  + S  C NNSV+ KLVKGKILIC+       F   GG AGVL + ++  D A
Sbjct: 367 VPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 426

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S P+PS+ LD NDA   + YI+S  SPTATIFKS    N  AP + SFSSRGPN ITK+
Sbjct: 427 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 486

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 487 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 546

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + +AEFAYG+GHVN LKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 547 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 606

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 607 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 666

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 667 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 726

Query: 721 ITSL 724
           +TSL
Sbjct: 727 VTSL 728

BLAST of HG10014859 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 2.3e-307
Identity = 541/732 (73.91%), Postives = 603/732 (82.38%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSF---- 60
           MSSSLIFKL F ++FFS  LAS LDS DD K IY+VYMGRKL +D DSAH H  +     
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60

Query: 61  ---PFAPESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLG 120
               FAPESV++TYKRSFNGFAVKLT+EEAEKIA MEGVVSVF N+MN+LHTTRSWDFLG
Sbjct: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120

Query: 121 FPKNVPRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKII 180
           FP  VPR  QVE+N++VGVLD+GIWPESPSFDD+ F P P KWKG+C+ + NF CNRKII
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180

Query: 181 GARAYRIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARI 240
           GAR+Y IGR +  GDV+ PRDTNGHGTHTASTAAGGL+SQA+ YGLGLGT RGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240

Query: 241 AVYKICWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGI 300
           A YK+CWNDGCSD D+LAA+DDAIADGVDIISLSVG    R YF+D+IAIGSFHA + GI
Sbjct: 241 AAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300

Query: 301 LTSNSAGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ 360
           LTSNSAGN GP  FTT SLSPWLLSVAASTMDRKF+T+VQIGN  SF+GVSINTFD N  
Sbjct: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQY 360

Query: 361 YPLVAGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFF-NFGGTAGVLT 420
           YPLV+G D+    F  STSR C +NSV PKL+KGKI++C+      +FF +  G AGVL 
Sbjct: 361 YPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420

Query: 421 VSDTRDTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSR 480
            S+TRD A S PLPSS LDPND      YI+S RSP ATIFKS T+ N SAP + SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480

Query: 481 GPNAITKDLVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYV 540
           GPN  TKD++KPD+S PGVEILAAWP VAPVGG  R TL+NIISGTSMSCPHITGIA YV
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYV 540

Query: 541 KTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYV 600
           KT+NPTWSPAAIKSALMTTASPMNA+F+P+AEFAYGSGHVN LKAVRPGLVYDANESDYV
Sbjct: 541 KTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYV 600

Query: 601 KFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSV 660
           KFLCGQGY T  VR IT D SACT  N GRVWDLNYPSFGL VS SK F+QYF RTLTSV
Sbjct: 601 KFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSV 660

Query: 661 ASQASTYRATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGV 720
           A QASTYRA IS P GL+I VNPNVLSFNG+GDRKSF LTVRG+++ ++VSASLVWSDGV
Sbjct: 661 APQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGV 720

Query: 721 HTVRSPITITSL 724
           H+VRSPITITSL
Sbjct: 721 HSVRSPITITSL 730

BLAST of HG10014859 vs. ExPASy TrEMBL
Match: A0A5D3E4N6 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00100 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 3.3e-306
Identity = 537/724 (74.17%), Postives = 603/724 (83.29%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SLI KLVF N+FF TLLASSLDS  DDK+IY+VYMG+K KDD D A+ H SSFPFAP
Sbjct: 1   MSFSLILKLVFFNLFFRTLLASSLDS--DDKEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA MEGVVSVFPN+MN  HTTRSWDF+GF +NVPR
Sbjct: 61  ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQVE+NV+VGVLDSGIWPESPSF+D+ FGP PSKWKG+C A+NFTCNRKIIGAR+Y IG
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LP GDV+ PRDTNGHGTHTAST AGGL+SQAS YGLGLGT RGGVPSARIAVYK+CW 
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCWR 240

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D CSD D+LAAFDDAIADGVDIISLSVG   +R YF+DSIAIGSFHA + GILTSNSAGN
Sbjct: 241 DACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAGN 300

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
           +GPKL TT SLSPWLLSVAAST+DRKF+TKVQIGN+NSF+ + +  F        +  + 
Sbjct: 301 NGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQVILLIFF--------ILFFS 360

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTV-SDTRDTA 420
            L   F  + +  C NNSV+ KLVKGKILIC+       F   GG AGVL + ++  D A
Sbjct: 361 KLYLIFVKTWNSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDNA 420

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S P+PS+ LD NDA   + YI+S  SPTATIFKS    N  AP + SFSSRGPN ITK+
Sbjct: 421 RSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITKE 480

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVA VGG HR TLYNI+SGTSMSCPHITGIA YVKTFNPTWS
Sbjct: 481 IIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTWS 540

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + +AEFAYG+GHVN LKAVRPGLVYDANESDYVKFLCGQGY
Sbjct: 541 PAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 600

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           TTN+VR ITNDNSACT+ NIGRVWDLNYPSFGL VS S+ F+QYFTR LT+VASQASTYR
Sbjct: 601 TTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTYR 660

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS+P GL+I VNP VLSFNGIGDRKSF LTV+GT+++ +VSASLVW DGVH+VRSPIT
Sbjct: 661 AAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPIT 714

Query: 721 ITSL 724
           +TSL
Sbjct: 721 VTSL 714

BLAST of HG10014859 vs. ExPASy TrEMBL
Match: A0A1S4E3D9 (LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=3 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 7.3e-306
Identity = 538/724 (74.31%), Postives = 599/724 (82.73%), Query Frame = 0

Query: 1   MSSSLIFKLVFLNIFFSTLLASSLDSADDDKKIYVVYMGRKLKDDRDSAHSHQSSFPFAP 60
           MS SL+F LVFLN+FFSTLLAS+LDS D+ +KIY+VYMG+KLKDD DSA+ H SSFPFAP
Sbjct: 7   MSFSLLFNLVFLNLFFSTLLASTLDSDDNGRKIYIVYMGKKLKDDPDSANLHHSSFPFAP 66

Query: 61  ESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNVPR 120
           ESV+YTY RSFNGFAVKLTKEEA+KIA M+GVVSVFPN++N+LHTTRSWDF+GFP+NV R
Sbjct: 67  ESVIYTYNRSFNGFAVKLTKEEADKIAGMKGVVSVFPNEINKLHTTRSWDFMGFPQNVRR 126

Query: 121 VKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQAMNFTCNRKIIGARAYRIG 180
           VKQV +N++VGV DSGIWPESPSF+DK F P PSKWKG+C A NFTCNRKIIGARAY IG
Sbjct: 127 VKQVGSNIVVGVFDSGIWPESPSFNDKGFDPPPSKWKGTCSAFNFTCNRKIIGARAYHIG 186

Query: 181 RSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWN 240
           R LPHGDV+ PRDT+GHGTH AS A GGL+++AS  GLGLGT RGG+PSARIAVYKICWN
Sbjct: 187 RPLPHGDVEGPRDTDGHGTHCASIAPGGLVNKASLNGLGLGTARGGIPSARIAVYKICWN 246

Query: 241 DGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGN 300
           D    +DLLAAFDDAI+DGVDIISLSVG   SR YF D IAIGSFHA QN ILTSNSAGN
Sbjct: 247 D--DXMDLLAAFDDAISDGVDIISLSVGGNISRKYFRDPIAIGSFHAIQNNILTSNSAGN 306

Query: 301 SGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQYPLVAGYD 360
            GP ++T TSLSPWLLSVAASTMDRKF+TKVQIGNK S +GVSINTF   GQYPLVA  D
Sbjct: 307 WGPNVYTVTSLSPWLLSVAASTMDRKFVTKVQIGNKRSIQGVSINTFGTTGQYPLVAARD 366

Query: 361 VLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVS-DTRDTA 420
           V    F + TS  C NNSVN KLVKGKIL C+       F +FGG AGVL V+ +  D A
Sbjct: 367 VPNNGFDNLTSTYCFNNSVNLKLVKGKILFCESSFHPVLFSSFGGVAGVLMVNVNPLDDA 426

Query: 421 FSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKD 480
            S PLPSS L+ +DA TIF YI S RSP A+I +S  + N  AP + SFSSRGPN +TK+
Sbjct: 427 LSFPLPSSVLNFHDAITIFDYIRSTRSPNASILRSTAVRNEPAPVVVSFSSRGPNNLTKE 486

Query: 481 LVKPDLSAPGVEILAAWPPVAPVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWS 540
           ++KPDLS PGVEILAAWPPVAPVG  +R TLYNIISGTSMSCPHIT IA YVKTFNPTWS
Sbjct: 487 IIKPDLSGPGVEILAAWPPVAPVGEINRNTLYNIISGTSMSCPHITEIAAYVKTFNPTWS 546

Query: 541 PAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGY 600
           PAAIKSALMTTA PMNA  + EAEFAYGSGHVN  +A+RPGLVYDANE DY+KFLCGQGY
Sbjct: 547 PAAIKSALMTTALPMNATLNLEAEFAYGSGHVNPKRAIRPGLVYDANEIDYIKFLCGQGY 606

Query: 601 TTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSVASQASTYR 660
           T  +V IIT+   AC S NIGRVWDLNYPSFGL VSHSK F QYF RTLTSVASQAS Y+
Sbjct: 607 TNGMVEIITSYEDACNSSNIGRVWDLNYPSFGLSVSHSKTFKQYFRRTLTSVASQASKYK 666

Query: 661 ATISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIVSASLVWSDGVHTVRSPIT 720
           A IS P GL I VNPNVLSFNGIGD+KSFKL VRGT+++ IVSASLVWSDGVH+VRSPIT
Sbjct: 667 AMISAPRGLVITVNPNVLSFNGIGDKKSFKLKVRGTIKESIVSASLVWSDGVHSVRSPIT 726

Query: 721 ITSL 724
           I SL
Sbjct: 727 INSL 728

BLAST of HG10014859 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 629.8 bits (1623), Expect = 2.7e-180
Identity = 348/721 (48.27%), Postives = 466/721 (64.63%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           LL+S     D+D ++Y+VYMG    + D      H S       ES     +V +YKRSF
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 138
           NGFA +LT+ E   IA++EGVVSVFPNK+ QLHTT SWDF+G    KN  R   +E++ I
Sbjct: 78  NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+D+GIWPES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RDT+GHGTHTASTAAG  +   SF+G+G GTVRGGVP++RIA YK+C + GCS   L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L++FDDAIADGVD+I++S+GF+    +  D IAIG+FHA   GILT +SAGNSGPK  T 
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 378
           + ++PW+ +VAAST +R FITKV +GN  +  G S+N FDM G +YPLV G     ++  
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             T+  C    +N   VKGKIL+C     Y    + G  A ++  S   D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHLPAS 437

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D  ++  YI S  SP A + K+ T+ N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R+  Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557

Query: 559 LMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 618
           +MTTA P+ AK       EFAYG+GHV+ + A+ PGLVY+ +++D++ FLCG  YT+  +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617

Query: 619 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 678
           +II+ D   C+ KN     +LNYPS    +S +   F   F RTLT+V +  STY++ + 
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677

Query: 679 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 721
             +G  LSIKV P+VL F  + +++SF +TV G+     V  SA+L+WSDG H VRSPI 
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 728

BLAST of HG10014859 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 624.4 bits (1609), Expect = 1.1e-178
Identity = 345/719 (47.98%), Postives = 463/719 (64.39%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           LL+S     D+D ++Y+VYMG    + D      H S       ES     +V +YKRSF
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 138
           NGFA +LT+ E   IA++EGVVSVFPNK+ QLHTT SWDF+G    KN  R   +E++ I
Sbjct: 78  NGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+D+GIWPES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RDT+GHGTHTASTAAG  +   SF+G+G GTVRGGVP++RIA YK+C + GCS   L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L++FDDAIADGVD+I++S+GF+    +  D IAIG+FHA   GILT +SAGNSGPK  T 
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 378
           + ++PW+ +VAAST +R FITKV +GN  +  G S+N FDM G +YPLV G     ++  
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             T+  C    +N   VKGKIL+C     Y    + G  A ++  S   D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHLPAS 437

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D  ++  YI S  SP A + K+ T+ N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R+  Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557

Query: 559 LMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIVRI 618
           +MTTA     +     EFAYG+GHV+ + A+ PGLVY+ +++D++ FLCG  YT+  ++I
Sbjct: 558 IMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKI 617

Query: 619 ITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATISTP 678
           I+ D   C+ KN     +LNYPS    +S +   F   F RTLT+V +  STY++ +   
Sbjct: 618 ISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAG 677

Query: 679 NG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPITI 721
           +G  LSIKV P+VL F  + +++SF +TV G+     V  SA+L+WSDG H VRSPI +
Sbjct: 678 HGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVV 723

BLAST of HG10014859 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 623.6 bits (1607), Expect = 2.0e-178
Identity = 348/721 (48.27%), Postives = 464/721 (64.36%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           LL+S     D+D ++Y+VYMG    + D      H S       ES     +V +YKRSF
Sbjct: 18  LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSF 77

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFP--KNVPRVKQVENNVI 138
           NGFA +LT+ E   IA  EGVVSVFPNK+ QLHTT SWDF+G    KN  R   +E++ I
Sbjct: 78  NGFAARLTESERTLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTI 137

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+D+GIWPES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 138 IGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TS 197

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RDT+GHGTHTASTAAG  +   SF+G+G GTVRGGVP++RIA YK+C + GCS   L
Sbjct: 198 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 257

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L++FDDAIADGVD+I++S+GF+    +  D IAIG+FHA   GILT +SAGNSGPK  T 
Sbjct: 258 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 317

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLVAGYDVLKTSFH 378
           + ++PW+ +VAAST +R FITKV +GN  +  G S+N FDM G +YPLV G     ++  
Sbjct: 318 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 377

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             T+  C    +N   VKGKIL+C     Y    + G  A ++  S   D AF++ LP+S
Sbjct: 378 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRPDVAFTHHLPAS 437

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D  ++  YI S  SP A + K+ T+ N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 438 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 497

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R+  Y++ SGTSM+CPH+ G+A YVKTF P WSP+ I+SA
Sbjct: 498 PGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSA 557

Query: 559 LMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 618
           +MTTA P+ AK       EFAYG+GHV+ + A+ PGLVY+ +++D++ FLCG  YT+  +
Sbjct: 558 IMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTL 617

Query: 619 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 678
           +II+ D   C+ KN     +LNYPS    +S +   F   F RTLT+V +  STY++ + 
Sbjct: 618 KIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVV 677

Query: 679 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 721
             +G  LSIKV P+VL F  + +++SF +TV G+     V  SA+L+WSDG H VRSPI 
Sbjct: 678 AGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIV 726

BLAST of HG10014859 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 617.8 bits (1592), Expect = 1.1e-176
Identity = 337/721 (46.74%), Postives = 462/721 (64.08%), Query Frame = 0

Query: 19  LLASSLDSADDDKKIYVVYMGR-KLKDDRDSAHSHQSSF-PFAPES-----VVYTYKRSF 78
           L  SS+ +  DDK++Y+VYMG    + D      H +       ES     +V +YKRSF
Sbjct: 17  LFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSF 76

Query: 79  NGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNV--PRVKQVENNVI 138
           NGFA +LT+ E E++A M GVVSVFPNK  QL TT SWDF+G  + +   R   VE++ I
Sbjct: 77  NGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTI 136

Query: 139 VGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAYRIGRSLPHGDV 198
           +GV+DSGI PES SF DK FGP P KWKG C    NFTCN K+IGAR Y           
Sbjct: 137 IGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY---------TS 196

Query: 199 DSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKICWNDGCSDIDL 258
           +  RD +GHGTHTASTAAG  +  ASF+G+G GTVRGGVP++R+A YK+C   GCS   L
Sbjct: 197 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 256

Query: 259 LAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNSAGNSGPKLFTT 318
           L+AFDDAIADGVD+I++S+G +T+  +  D IAIG+FHA   G+LT NSAGNSGPK  + 
Sbjct: 257 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 316

Query: 319 TSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNGQ-YPLVAGYDVLKTSFH 378
           + ++PW+L+VAAST +R F+TKV +GN  +  G S+N ++M G+ YPLV G     ++  
Sbjct: 317 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 376

Query: 379 DSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTRDTAFSNPLPSS 438
             ++  C  + V+   VKGKIL+C             G  G++  +   D AF +PLP++
Sbjct: 377 AESAGLCELSCVDKSRVKGKILVCGGPGGL-KIVESVGAVGLIYRTPKPDVAFIHPLPAA 436

Query: 439 TLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAITKDLVKPDLSA 498
            L   D +++  Y+ S  SP A + K+  + N ++P +ASFSSRGPN I  D++KPD++A
Sbjct: 437 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 496

Query: 499 PGVEILAAWPPVA-PVGGSHRKTLYNIISGTSMSCPHITGIAVYVKTFNPTWSPAAIKSA 558
           PGVEILAA+ P   P     R   Y+++SGTSMSCPH+ G+A YVKTFNP WSP+ I+SA
Sbjct: 497 PGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSA 556

Query: 559 LMTTASPMNAKFD--PEAEFAYGSGHVNSLKAVRPGLVYDANESDYVKFLCGQGYTTNIV 618
           +MTTA P+NA        EFAYGSGHV+ + A  PGLVY+ ++SD++ FLCG  YT+ ++
Sbjct: 557 IMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVL 616

Query: 619 RIITNDNSACTSKNIGRVWDLNYPSFGLFVSHS-KIFDQYFTRTLTSVASQASTYRATIS 678
           ++I+ +   C+        +LNYPS    +S S   F   F RTLT+V +  STY + + 
Sbjct: 617 KVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVV 676

Query: 679 TPNG--LSIKVNPNVLSFNGIGDRKSFKLTVRGTMRKYIV--SASLVWSDGVHTVRSPIT 721
             +G  L +K+ P+VLSF  + +++SF +TV G+     V  SA+L+WSDG H VRSPI 
Sbjct: 677 AGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIV 727

BLAST of HG10014859 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 617.8 bits (1592), Expect = 1.1e-176
Identity = 325/675 (48.15%), Postives = 452/675 (66.96%), Query Frame = 0

Query: 59  APESVVYTYKRSFNGFAVKLTKEEAEKIADMEGVVSVFPNKMNQLHTTRSWDFLGFPKNV 118
           A   +V +YKRSFNGFA  L++ E++K+ +M+ VVSVFP+K ++L TTRSWDF+GF +  
Sbjct: 28  ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA 87

Query: 119 PRVKQVENNVIVGVLDSGIWPESPSFDDKDFGPIPSKWKGSCQ-AMNFTCNRKIIGARAY 178
            R    E++VIVGV+DSGIWPES SFDD+ FGP P KWKGSC+  + F CN K+IGAR Y
Sbjct: 88  RRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY 147

Query: 179 RIGRSLPHGDVDSPRDTNGHGTHTASTAAGGLLSQASFYGLGLGTVRGGVPSARIAVYKI 238
                  +   DS RD  GHGTHTASTAAG  +  ASFYGL  GT RGGVPSARIA YK+
Sbjct: 148 -------NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKV 207

Query: 239 CWNDGCSDIDLLAAFDDAIADGVDIISLSVGFETSRPYFLDSIAIGSFHATQNGILTSNS 298
           C+N  C+D+D+LAAFDDAIADGVD+IS+S+  +        S+AIGSFHA   GI+T+ S
Sbjct: 208 CFN-RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGS 267

Query: 299 AGNSGPKLFTTTSLSPWLLSVAASTMDRKFITKVQIGNKNSFEGVSINTFDMNG-QYPLV 358
           AGN+GP   +  ++SPW+++VAAS  DR+FI +V +GN  +  G+S+NTF++NG ++P+V
Sbjct: 268 AGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIV 327

Query: 359 AGYDVLKTSFHDSTSRSCRNNSVNPKLVKGKILICKELLSYDDFFNFGGTAGVLTVSDTR 418
            G +V + +   + +  C +  V+ +LVKGKI++C + L Y + +  G    ++  +   
Sbjct: 328 YGQNVSR-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLP 387

Query: 419 DTAFSNPLPSSTLDPNDADTIFHYIHSARSPTATIFKSNTLHNVSAPFLASFSSRGPNAI 478
           D+AF  P P+S+L   D  +I  YI SA  P A I ++  + +  AP++ SFSSRGP+ +
Sbjct: 388 DSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFV 447

Query: 479 TKDLVKPDLSAPGVEILAAWPPVAPVGG-----SHRKTLYNIISGTSMSCPHITGIAVYV 538
            ++L+KPD+SAPG+EILAA+ PVA           R   Y+++SGTSM+CPH+ G+A YV
Sbjct: 448 IQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYV 507

Query: 539 KTFNPTWSPAAIKSALMTTASPMNAKFDPEAEFAYGSGHVNSLKAVRPGLVYDANESDYV 598
           K+F+P WSP+AIKSA+MTTA+PMN K +PE EFAYGSG +N  KA  PGLVY+    DY+
Sbjct: 508 KSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYL 567

Query: 599 KFLCGQGYTTNIVRIITNDNSACTSKNIGRVWDLNYPSFGLFVSHSKIFDQYFTRTLTSV 658
           K LC +G+ +  +   +  N  C+ +    V DLNYP+   FVS    F+  F RT+T+V
Sbjct: 568 KMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNV 627

Query: 659 ASQASTYRAT-ISTPNGLSIKVNPNVLSFNGIGDRKSFKLTVRGTMRK--YIVSASLVWS 718
               STY+A+ +     L I + P +L F  + ++KSF +T+ G   K    VS+S+VWS
Sbjct: 628 GFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWS 687

Query: 719 DGVHTVRSPITITSL 724
           DG H+VRSPI   S+
Sbjct: 688 DGSHSVRSPIVAYSI 691