HG10014727 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10014727
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha-galactosidase
LocationChr02: 18756489 .. 18763535 (+)
RNA-Seq ExpressionHG10014727
SyntenyHG10014727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGAGGGTAAGGTTTGAATCAGTATTTTTACTGATTCTATCACTCTCGGCGGCGTCTTTGGCGATTGCAGAGAGAAGAGTGTCACTGTTGAATGGTTATGATAAATCGATTTTCAAGTCATCATTTCATCGGATTTTTGATACCTCCAAGTACGGCATATTGCAGCTTCAAAATGGCTTGGCTCGAACGCCTCAGATGGGGTATCTTTCTTTTTCCATATCTCTGAATTTTCTAAGTCTTTTATGATTTTCCCTATCTTTTGAACAACTGGGTTATAGTTCATTTCAAATTTTTTATCATGGAAGAAGCTTAGAGGTGATTTGGAGTTTCATTTGGGAGGGGAACTTGTAGAAACTTTTCAATTTATGGACGATTGGGGGAATCGAGTTTTGTTTGTGGGCTTTGCCAAACAGTTTATGGACTTTATGTTTTTAATGATCAGTGATTTTCAGGTTTGCTTTGAAGCTTATAATTGTCTTTATGTATCCTTTCAGATGGAATAGCTGGAATTTTTTCGCCTGTGATATTAATGAAACTCTTATCAAGGAAACTGGTATCTCTCTTCCTTTCTTTTTTCCCCTCATATTCTGTGTACTGGGTGATTTGGAAAAGCTGCAGTGAAAGCTCAATATGATTAGTACATCTTTAAGTTTATTGGTTACATTTTGCAGGTTAATTTGCTTTGGAAAGCAATATTTCCTGTTCGAAATGAAAGATTAATATACTGAAGTTTCTATGAGACCGCTAATATTTACTTGAGTTAATTTGCACATTTTTTCCTTTTCAAACTTGTTAGTTTATCCTGCTAAAATTAGTTTACTCGTGAAGTTTATAACTTATGTTAATTCATCGTATTATTGTGCATGCTACCTGAGGTATATTGAGTTCTTTAATCCATAAAGTTTGGAATTCAATAAATCTAATTGAAGTCATTGTTTCCCTGAAAAATGTGACTTCTCTGTTGGAAGTTTCTTATTTGAAGATCATCACTTTGCAATATTTGAGTTGTGTGTTAGTTATTCCTCAATAATAATGGTCTTTTTCTTTCTTGCTTCTGTTTCAACATTATTTGGATGTTGGTTTTGACAGCGGATGCACTTGTTTCTTCGGGTCTGGCTGAGTTAGGTTATGTGTACGTCAACATCGGTACATTTCCAGCTTGTTTGAAGTGCCAACTTTTCTTTACGTTGTGTTTTAATTTAGTACTTATTATATTTTAAATAAGTGCAGATGATTGCTGGAACACTCAAAAGAGAGATTCAAAGGTTATTTCAATTCCATTTTGTGGAAGAAGATTTGACTTTCCAATGTTTGAAGATTGTTTTAACAAAAAACATTGGATGGAGTTGTAATTCTCCAATTTAATATTGATCCTACTCTGCCTGTGAAATTCCTTGAGGCCCATTGTGTAAATTAATCTTGTATGAATGTGAAGTGACAAACTATTCGAACTCAGTGATGCTATTATAGAAACTGCCAGCAATATCTTACAAGAAAAGAGTTCTACATGATAAACCATGGAATAGGGCTTTTCAGTGCCATTGTTCCTTGTTAAGGCTCCCATCCTTGTATGTGCAGGATCAAATGGTTCCTGATCCCAAGGCTTTCCCGTCAGGAATTAAACCTGTTGCTGATTATGTACATAGTAAAGGCTTGAAGCTTGGAATCTATTCTGATGCTGGGTAGGAAGAAATGTCAACTTAACTTCTAAAGCTTAAGAGTTAATGTTATCTCTCTCTTTTGCTTCTCATATATTACTTTATAGAAAGTGCATGCTGATGACTTTTATTTTGATTCTCTTACACCTGACAGTCTTTTCACCTGCCAAGTTCGAGCAGGGTCACTTTACCATGAAAATGATGATGCACAGTTGTTTGCTTCTTGGGTGAGTAATTCTAATTTAACTTTTTATATTGGAAATATGTATTCAATACTTTTCTTAAGAAGCAGTATGTATGGTTTACGTATACGGGTTTGCTTAGTGGTTCTTGGGGCAAGTATAAAAAAAGTAAAAGGATGACTTTGAGAAAATGAGTTCAAACCATGGTGGCTACCTACCTAAGGCGTTAAAATCATACCAGTTTTGTAACGACCAAATGTTAAGGGGGAAAAAGAAGTAATTTGTAGATATGCTTTTTGAGATTATTTTTCTCTGGCCTCATCATCCTCAATCAGTAAAGAATTTTGTCAATTGTCTTGTTCTTAACAATTTTTAATGCTTCCTTGTAGTCACAGTCCTACCTTTTCGAGGGCTGGACATCTTTTCTCCCTCCAAGATGGAATTTTATAGCCTAATTTGACCTTATTTCTTCGTAAATCATTGGCCTATTGGAATATACACTTTTTTCTTGTTTGAGTGACTTATTGGAGCGTGCTTCCACAAAACATAATATGTAAATTTTAGATTGACGTCTTCCTTTTTTAATGAATAAACGTTTATTTATTTTGAAATTCTAGGTTGTCCATAGAATTCTACTATCACTTCAGTTTTTAAGTTCATATTATTCCATCTGATTTCATAATTTTCTTCATATTCTTTTTTTACTAGGGGATTGATTATTTGAAGTATGATAACTGTTACAATCTGGGAATTAAACCAATAGAGCGGTTTGTACCTCATTATACTCAATAGTTTATTTGATTGTAAATTGGAAGCTTCTTAGTTCTTACTGATTTGAAGTTAACTTATGACTTTCAGATTCCCACCTATGCGTGATGCACTAAATGCTACTGGGCGAAGTATTTTCTATTCAATTTGTGAATGGTACGCTTGTTCTTATAGACATCTTTGCTGATTTTTTAATTTTCTTTTTAAACTTTTTGTTGCTATCATCTTTGAATTTATTGGGCTATGATTTTGCTGCTGGCAGGGGTGTTGATGATCCAGCATTATGGGCCGGCAAGGTTGGAAACAGTTGGCGTACAACAGATGACATCAATGATACATGGGCAAGGTCAGGTTCTAGGCCTTTCCATCAATCTTCCTTCTTTACTCGAGATTTTTAATCTTCTGTAATTCCCCTCGATTCATTTTATGTTGACAAACACCATATTTTAGCACATTGCTCGAACTTCTGAGCAGAAAAACTTTTGAACTGGTTAGAAAATATTGGAAGTTTTTTTTTAGCTTTATTACTTTGCCTGCATAGAGTTGCATTTCAAGTTTTTTGATGGAACTTAGAATTAAGACAGTTTTCCTTTGTACTGATTTTAAATTCTTAAAAAATTATTTGTTAGTTGTGATGCCAAATTTTTGTGACGTGGTTTGAAAGCTCTACCGCTACCATATCTTTCTTCTCTTTCATTTTCAGGTGGTGCTAATATGATGCTGATTGGTTTTTCTTGTGTTTTCCTTTTTTGTTCAAGCATGACTACTCTTGCAGATATCAACAATAAGTGGGCAGCTTATGCAGGACCTGGTGGCTGGAATGGTAGGAATATGATTTTGTCTAAAGGTGTTGATTTCTCTGTTTGTTTATGAGGCTTGCTATTTATTTTACCCTTTTGTGGACAGATCCGGATATGTTGGAAGTTGGTAATGGAGGCATGACTTATCAGGAATATCGTGCTCATTTTAGCGTATGGGCTTTGATGAAGGTTTCTCCATCCCTCTCTCTTTATCACATGACTTGTATGATAAATTCCTATTACAGTTAGTCTACTCATCCGTTGTTTTTTCTACAGCCCTACATATGCATATTCATATAGAAATGTCAATCCTTTGACCATCCTACGTTTATATTTCTTTGTATTTTTAAACAAATGATTCTCTCGAATCTAGTTCCACATAGATCATAAACTGCCTCCCGCCTTCCCTACTTGTCAGAGATGCTTCAGCATTCTCTTTCTGGAGAAACTAGTTTCCACAATGACTTGAAGGCACATACGTCAATAATAAGTTGTGTAGAATTCTATGCTGGTAGGGTAGTTTTATACTTATCGGTGTATTAGTCAGTAGATGTATATACTAATACATGTTCTGTTTTAGAGTGACAGATAAGGTAGTTAGGAAAATTACAAGGTTAAGGGAATTAATATCCTGCCTGAACAAAAGAAATAATAGAACTTCAATAATTGATAGGGTAATTTTGTACTGTCATCTTTGGCAATTTCTGTACATCTCTCCTCAAGTTGGTTAGAAGATTATTCTCTTACTAGAACTAGGAGCTCGTTATATCGAATAAAAAAGTTGAATTAGATTTCTTTCGGGAAATGTTTGATTATGACATCTTTTAGGAATGTTGGAATAAATATTCGTCTACTATCACAAACTTTCATATTGTTTATAGTAGCTGATGGTGAAAGTTAAGTAGCCAGTCCAAGCTATTCTATCATTGTTTCTAAATTCATTCTCTGCAGTTGCAGACAACTGCCCATTGTTTGATGTTCCTTCGTATTGCCAAATTACCTTGAAAATAAATTGATACCATTGTGTAGATCTCCTATTTGTTAAATTTTGTCCAATGGTGTTTTTACATATTGTTTCGTGACGTTGAGAATAAAATCCATTTTCTATGCGACTTTTGATTGTGTTTCAAGAATTTGCTCAAGTATATGGAGTGTTGTTCAAGTTCTTCCTTTGGACAATGGTTTAAGGGGAGAGTTAAACCAAGACTGATGGGAGTTAAAAGAAGATATCCCCTCATGGTTCCCAATCCTCAAATATGTATTTCACTTCCACAAAAAGTCTCTGAGAAGTGAGAAATGTTATGGGGGCTTTCAATCTCAATTGTGTGGCTCCTCATCACATGGAAGAGGACTAATCACTTTCGGCCAATGCCACTTATATTAAGATTAACTTGACATTTTTTGGGAGCTGGAAGGGAATACGGCATTATGTTATGTTGGACTATAAATACTAAAATCTTTGGTTGTTTGACATCATTCTGCAATATGTGTAGAAGCATGCGTGTTTCGCAATCTTCAAACTCTACTGATTGGGACCCTTGTCACACCTTGTACATGTTTACTAATTGATCTAGTTTTTTCCTTTTATATGCCTGTTTACAATTTTAGCCTTAATGTGCAGTCCCCTCTTTTGATCGGTTGCGATGTTCGAAATATGACGAAAGAAACTTCAGAAATTCTTAGCAATAAGGAGGTCATTGCAGTAAACCAAGGTGAACCATATGTTTAAATTCTTTCATATCTCATTTTCTTAAGCTGAAGCCCTCTCTTCGTACTTGTGTAGTGTTTGTCAGCTTTTTGGTTGTTTTTCTTGCATCTTTCTGTCCTGGCCTTTTCTCTTTCTTTTGTATTGCTTTGCATATTCTTTCATTTCATCGCCATGAAAGTTGGTTTCTTCATTTTAGAAATTATAAAAAATAAAAATGTGAATATCCACCAATAGAACTGTTTAGTCTAATTAATTGATCCTTTCTCAACATTGATACCACTGATATTTTCTCTCTTCAAATTACAATGTCCATGGATTATTATTTGCATACAACTTAAAATTGCTCACCAAGATAACCTATAATGTTCTTAACTTGGCTAGTTTGGCTTTTGGTTTTTGAAAATTGAGCTTCTTTTCTTTCTTTACCAATATTTTCATTTGTTATAAGAAAACATTTGAATTCATAACCAAACTCCAAACTCCAAGGAGACACACAACCAAAAAAAAAAGTTTTAAATCCCACTTTTTTAGTTTGCAAAACTTGCTTGGATTTTGAAAATATCTCTAAAATTAGATAACAAAACAAAGCAATCCATAGGTGGAAGTAGTGTTTCTAAGATTAATTTTTAAAAAATAAAAAATTAAACACCAAATGGATATCAAACGAGGCCTTAATTATTTGTTTGTCGAAAGTGAAAATAATTTGTTAGTGCTTATTATATGCAGATCCTCTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTATTAGTGTTTTTGCATTATCTTATAGATTAATATAGCAAGATGACACTGTAACACATAAAGATGGCAATATTTACTTGTATTTAATGTAGAATACTGTCAAGTTAATAACTGGAAATATCTTTACCTTCCAAACATGCTGTCAGTGTTCTAGTGAAAACTGAGAAGGCAAATTATTTTCTTGATATATTTGAGTTTTATTGCTGTTATGGTTTGTACATAATTTAACTAACCATGTCAGTTAGCTGAGTTAGGTGTGAATATTTCCTAATTTAAAGAGTTGTCTTACAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCTGTCATTCTTTGGAATCGGTGTTCGGTCGCATCAACGATCACCACGGATTGGAATGCACTTGGGCTTAAACCTGACACCAGTGTCTCAGTAAGAGACTTGTGGCAGGTAAGGGATCTGATCACTGAAATTCTTAACATTGAGATTATACTCATGGACACTCATTACTTTTGATGGATTTACGATTAGGATGTTAGGTTACTACCTAGCAAGAAAACTAAAGAACAACCAATAACACAAAAATACAGAAAGGAATATATATTGCAATATCAAAGAAGAAACTACAATATTATAGCCTTTCGAGAGGACAACACTCTTTTAAATTCCAAAAATGGAAATTCTCAAAATGATTTACACTTCGTTCCCAATCCACTCCCTCTATTTATAACCCAAAGACCTTAACCAACTTACTGGCTAAATACTAATATGGCCCTTGCTAATAACCATGACAATAATCCTATTATCTCCCTAACTAGGGGTCTTACATAGGAGTAGTTGGAGCTTGTACAATCGACACATGATAGAACAACTGGTCTCTAACAAAACCTATCTTAATAGTCTGCCCATATTCTTCTCTAATTTCTTTTCATTAGGTAGGAGAGGAGACTCAATGGTTGGGTCCATTTTATGTCCTGCCTTCCTTTTAATTCGCTTTTCGACCACCTACCCTTTTTGGGTGCTAACTTGGTAATTCGACTTTTGCAGCACGAAGATATTGCAGGAGATGCAGTGGCATCATTTGGAGCAGAAGTCGATCCTCACGACTGCAAAATGTTTGTCTTCACACCTGTGGCCACGTCTCGTGCTGAAATGTAG

mRNA sequence

ATGGCGAAGAGGGTAAGGTTTGAATCAGTATTTTTACTGATTCTATCACTCTCGGCGGCGTCTTTGGCGATTGCAGAGAGAAGAGTGTCACTGTTGAATGGTTATGATAAATCGATTTTCAAGTCATCATTTCATCGGATTTTTGATACCTCCAAGTACGGCATATTGCAGCTTCAAAATGGCTTGGCTCGAACGCCTCAGATGGGATGGAATAGCTGGAATTTTTTCGCCTGTGATATTAATGAAACTCTTATCAAGGAAACTGCGGATGCACTTGTTTCTTCGGGTCTGGCTGAGTTAGGTTATGTGTACGTCAACATCGATGATTGCTGGAACACTCAAAAGAGAGATTCAAAGGATCAAATGGTTCCTGATCCCAAGGCTTTCCCGTCAGGAATTAAACCTGTTGCTGATTATGTACATAGTAAAGGCTTGAAGCTTGGAATCTATTCTGATGCTGGTCTTTTCACCTGCCAAGTTCGAGCAGGGTCACTTTACCATGAAAATGATGATGCACAGTTGTTTGCTTCTTGGGGGATTGATTATTTGAAGTATGATAACTGTTACAATCTGGGAATTAAACCAATAGAGCGATTCCCACCTATGCGTGATGCACTAAATGCTACTGGGCGAAGTATTTTCTATTCAATTTGTGAATGGGGTGTTGATGATCCAGCATTATGGGCCGGCAAGGTTGGAAACAGTTGGCGTACAACAGATGACATCAATGATACATGGGCAAGCATGACTACTCTTGCAGATATCAACAATAAGTGGGCAGCTTATGCAGGACCTGGTGGCTGGAATGATCCGGATATGTTGGAAGTTGGTAATGGAGGCATGACTTATCAGGAATATCGTGCTCATTTTAGCGTATGGGCTTTGATGAAGTCCCCTCTTTTGATCGGTTGCGATGTTCGAAATATGACGAAAGAAACTTCAGAAATTCTTAGCAATAAGGAGGTCATTGCAGTAAACCAAGATCCTCTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCTGTCATTCTTTGGAATCGGTGTTCGGTCGCATCAACGATCACCACGGATTGGAATGCACTTGGGCTTAAACCTGACACCAGTGTCTCAGTAAGAGACTTGTGGCAGCACGAAGATATTGCAGGAGATGCAGTGGCATCATTTGGAGCAGAAGTCGATCCTCACGACTGCAAAATGTTTGTCTTCACACCTGTGGCCACGTCTCGTGCTGAAATGTAG

Coding sequence (CDS)

ATGGCGAAGAGGGTAAGGTTTGAATCAGTATTTTTACTGATTCTATCACTCTCGGCGGCGTCTTTGGCGATTGCAGAGAGAAGAGTGTCACTGTTGAATGGTTATGATAAATCGATTTTCAAGTCATCATTTCATCGGATTTTTGATACCTCCAAGTACGGCATATTGCAGCTTCAAAATGGCTTGGCTCGAACGCCTCAGATGGGATGGAATAGCTGGAATTTTTTCGCCTGTGATATTAATGAAACTCTTATCAAGGAAACTGCGGATGCACTTGTTTCTTCGGGTCTGGCTGAGTTAGGTTATGTGTACGTCAACATCGATGATTGCTGGAACACTCAAAAGAGAGATTCAAAGGATCAAATGGTTCCTGATCCCAAGGCTTTCCCGTCAGGAATTAAACCTGTTGCTGATTATGTACATAGTAAAGGCTTGAAGCTTGGAATCTATTCTGATGCTGGTCTTTTCACCTGCCAAGTTCGAGCAGGGTCACTTTACCATGAAAATGATGATGCACAGTTGTTTGCTTCTTGGGGGATTGATTATTTGAAGTATGATAACTGTTACAATCTGGGAATTAAACCAATAGAGCGATTCCCACCTATGCGTGATGCACTAAATGCTACTGGGCGAAGTATTTTCTATTCAATTTGTGAATGGGGTGTTGATGATCCAGCATTATGGGCCGGCAAGGTTGGAAACAGTTGGCGTACAACAGATGACATCAATGATACATGGGCAAGCATGACTACTCTTGCAGATATCAACAATAAGTGGGCAGCTTATGCAGGACCTGGTGGCTGGAATGATCCGGATATGTTGGAAGTTGGTAATGGAGGCATGACTTATCAGGAATATCGTGCTCATTTTAGCGTATGGGCTTTGATGAAGTCCCCTCTTTTGATCGGTTGCGATGTTCGAAATATGACGAAAGAAACTTCAGAAATTCTTAGCAATAAGGAGGTCATTGCAGTAAACCAAGATCCTCTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCTGTCATTCTTTGGAATCGGTGTTCGGTCGCATCAACGATCACCACGGATTGGAATGCACTTGGGCTTAAACCTGACACCAGTGTCTCAGTAAGAGACTTGTGGCAGCACGAAGATATTGCAGGAGATGCAGTGGCATCATTTGGAGCAGAAGTCGATCCTCACGACTGCAAAATGTTTGTCTTCACACCTGTGGCCACGTCTCGTGCTGAAATGTAG

Protein sequence

MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVFTPVATSRAEM
Homology
BLAST of HG10014727 vs. NCBI nr
Match: XP_038892272.1 (alpha-galactosidase 3 [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 2.1e-251
Identity = 414/430 (96.28%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RFESVFLLILSLSAAS+AIAERRVSLLNGYDKSIFKSSFHRI+DTSKYGILQLQN
Sbjct: 1   MANRIRFESVFLLILSLSAASVAIAERRVSLLNGYDKSIFKSSFHRIYDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFAC+IN+TLIKETADAL+SSGLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACNINDTLIKETADALISSGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI
Sbjct: 121 QLVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPIER+PPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIERYPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN L LKP+T VSVRDLW HEDIAGDAVASFGAEVDPHDCKMFVF
Sbjct: 361 VLWNRCSVASTITTDWNVLRLKPNTIVSVRDLWLHEDIAGDAVASFGAEVDPHDCKMFVF 420

Query: 421 TPVATSRAEM 431
           TPVATSRAEM
Sbjct: 421 TPVATSRAEM 430

BLAST of HG10014727 vs. NCBI nr
Match: KGN50728.1 (hypothetical protein Csa_005887 [Cucumis sativus])

HSP 1 Score: 864.4 bits (2232), Expect = 4.2e-247
Identity = 404/430 (93.95%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL LSAA +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLLSAAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDINETLIKETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKP+ADYVHSK LKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWG+
Sbjct: 121 QLVPDPKGFPSGIKPLADYVHSKDLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNC+NLGIKPI+R+PPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCFNLGIKPIKRYPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLAD+NNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADLNNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWNALGLKP+TSVSVRDLW HED+AGDA++SFGAEVDPHDCKMFVF
Sbjct: 361 VLWNRCSVASTITTDWNALGLKPNTSVSVRDLWLHEDVAGDAMSSFGAEVDPHDCKMFVF 420

Query: 421 TPVATSRAEM 431
           TPVAT RAEM
Sbjct: 421 TPVATFRAEM 430

BLAST of HG10014727 vs. NCBI nr
Match: NP_001292680.1 (alpha-galactosidase 3 precursor [Cucumis sativus] >AEQ94270.1 alpha galacdosidase 3 [Cucumis sativus])

HSP 1 Score: 864.0 bits (2231), Expect = 5.5e-247
Identity = 403/430 (93.72%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL LSAA +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLLSAAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDINETLIKETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKP+ADYVHSK LKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWG+
Sbjct: 121 QLVPDPKGFPSGIKPLADYVHSKDLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNC+NLGIKPI+R+PPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCFNLGIKPIKRYPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLAD+NNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADLNNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVKVFGKDGCLQ+WAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQIWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWNALGLKP+TSVSVRDLW HED+AGDA++SFGAEVDPHDCKMFVF
Sbjct: 361 VLWNRCSVASTITTDWNALGLKPNTSVSVRDLWLHEDVAGDAMSSFGAEVDPHDCKMFVF 420

Query: 421 TPVATSRAEM 431
           TPVAT RAEM
Sbjct: 421 TPVATFRAEM 430

BLAST of HG10014727 vs. NCBI nr
Match: XP_008456938.1 (PREDICTED: alpha-galactosidase 3 isoform X1 [Cucumis melo])

HSP 1 Score: 862.8 bits (2228), Expect = 1.2e-246
Identity = 405/430 (94.19%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL  S A +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLHSVAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDIN+TLI+ETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINDTLIQETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI
Sbjct: 121 QLVPDPKGFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPI+RFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIKRFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVK FGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKDFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN LGLKP+TSVSVRDLW HED+ GDAV+SFGAEVDPHDCKMF+F
Sbjct: 361 VLWNRCSVASTITTDWNVLGLKPNTSVSVRDLWLHEDVEGDAVSSFGAEVDPHDCKMFIF 420

Query: 421 TPVATSRAEM 431
           TPVATSRAEM
Sbjct: 421 TPVATSRAEM 430

BLAST of HG10014727 vs. NCBI nr
Match: XP_022985233.1 (alpha-galactosidase 3 [Cucurbita maxima] >XP_022985242.1 alpha-galactosidase 3 [Cucurbita maxima] >XP_022985247.1 alpha-galactosidase 3 [Cucurbita maxima])

HSP 1 Score: 847.4 bits (2188), Expect = 5.3e-242
Identity = 395/430 (91.86%), Postives = 417/430 (96.98%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MAKR+   SVFLLILSL+AAS A+AER VSLLNGYD+SIFKSSFHRIFDTSKYGILQL+N
Sbjct: 1   MAKRISSGSVFLLILSLTAASAAVAERGVSLLNGYDRSIFKSSFHRIFDTSKYGILQLEN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDI+E+LIKETADALVSSGLA+LGYVYVNIDDCW+T KRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDIDESLIKETADALVSSGLADLGYVYVNIDDCWSTLKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPKAFPSGIK VADYVHSKGLKLGIYSDAG+FTCQVR GSLYHENDDA+LFASWG+
Sbjct: 121 QLVPDPKAFPSGIKAVADYVHSKGLKLGIYSDAGIFTCQVRPGSLYHENDDAELFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVR+MTKET EILSN+EVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRSMTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN LGLKP TSVSVRDLW H+D+AGDAVASFGAEVDPHDCKMF+F
Sbjct: 361 VLWNRCSVASTITTDWNTLGLKPGTSVSVRDLWLHKDVAGDAVASFGAEVDPHDCKMFIF 420

Query: 421 TPVATSRAEM 431
           TP AT+RAEM
Sbjct: 421 TPAATARAEM 430

BLAST of HG10014727 vs. ExPASy Swiss-Prot
Match: Q8VXZ7 (Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.0e-191
Identity = 302/429 (70.40%), Postives = 359/429 (83.68%), Query Frame = 0

Query: 8   ESVFLLILSLSAASLAIAE------RRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQNG 67
           +SV  L++ L + S+ +++      +   L +     +F  SF+ I+DTS YG LQL NG
Sbjct: 9   DSVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNG 68

Query: 68  LARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKDQ 127
           LARTPQMGWNSWNFFAC+INET+IKETADALVSSGLA+LGY++VNIDDCW+   RDS+ Q
Sbjct: 69  LARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRDSEGQ 128

Query: 128 MVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGID 187
           +VP P+ FPSGIK +ADYVHSKGLKLGIYSDAG+FTC+V  GSL+HE DDA +FASWG+D
Sbjct: 129 LVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTCEVHPGSLFHEVDDADIFASWGVD 188

Query: 188 YLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTDD 247
           YLKYDNC+NLGIKPIER+PPMRDALNATGRSIFYS+CEWGVDDPALWA +VGNSWRTTDD
Sbjct: 189 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 248

Query: 248 INDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPLL 307
           INDTWASMTT+AD+NNKWAAYAGPGGWNDPDMLE+GNGGMTY+EYR HFS+WALMK+PLL
Sbjct: 249 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 308

Query: 308 IGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVI 367
           IGCDVRNMT ET EILSNKE+IAVNQDPLGVQGRK++  G++ C QVW+GPLSG R+ V 
Sbjct: 309 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 368

Query: 368 LWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVFT 427
           LWNRCS  +TIT  W+ +GL+   SVSVRDLWQH+D+  +   SF A+VD HDC M+V T
Sbjct: 369 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 428

Query: 428 PVATSRAEM 431
           P   S +++
Sbjct: 429 PQTVSHSDV 437

BLAST of HG10014727 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 5.3e-144
Identity = 232/368 (63.04%), Postives = 284/368 (77.17%), Query Frame = 0

Query: 59  QNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDS 118
           +NGL +TP MGWNSWN F CDINE +++ETADA+VS+GLA LGY Y+N+DDCW    RDS
Sbjct: 49  ENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAELNRDS 108

Query: 119 KDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVR-AGSLYHENDDAQLFAS 178
           +  MVP+  AFPSGIK +ADYVHSKGLKLG+YSDAG  TC  R  GSL HE  DA+ FAS
Sbjct: 109 EGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDAKTFAS 168

Query: 179 WGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWR 238
           WG+DYLKYDNC NLGI   ER+PPM  AL ++GR IF+S+CEWG +DP +WA  +GNSWR
Sbjct: 169 WGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSIGNSWR 228

Query: 239 TTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMK 298
           TT DI D W SMT++AD N+KWA+YAGPGGWNDPDMLEVGNGGMT +EYR+HFS+WAL K
Sbjct: 229 TTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSIWALAK 288

Query: 299 SPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSR 358
           +PLL+GCD+R M   T E++SN EVIAVNQD LGVQG+KVK       L+VWAGPLS ++
Sbjct: 289 APLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGKKVK---STNDLEVWAGPLSDNK 348

Query: 359 LAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHED---IAGDAVASFGAEVDPHD 418
           +AVILWNR S  +T+T  W+ +GL+  T+V  RDLW+H     ++G+      AE+D H 
Sbjct: 349 VAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHSTQSLVSGE----ISAEIDSHA 408

Query: 419 CKMFVFTP 423
           CKM+V TP
Sbjct: 409 CKMYVLTP 409

BLAST of HG10014727 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 6.5e-142
Identity = 233/365 (63.84%), Postives = 276/365 (75.62%), Query Frame = 0

Query: 58  LQNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRD 117
           + NGLA +PQMGWNSWN F C+INETLIK+TADA+VSSGL+ +GY Y+NIDDCW   KRD
Sbjct: 32  MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 91

Query: 118 SKDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTC-QVRAGSLYHENDDAQLFA 177
           S+  +V     FPSGIK ++DYVHSKGLKLGIYSDAG  TC Q   GSL HE  DA+ FA
Sbjct: 92  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 151

Query: 178 SWGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSW 237
           SWGIDYLKYDNC N G  P ER+P M  AL  +GRSIF+S+CEWG +DPA WAG +GNSW
Sbjct: 152 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 211

Query: 238 RTTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALM 297
           RTT DI D W SMT +AD N++WA+YA PG WNDPDMLEVGNGGMT +EY +HFS+WAL 
Sbjct: 212 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 271

Query: 298 KSPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGS 357
           K+PLLIGCD+R+M K T E+LSNKEVIAVNQD LG+QG+KVK   K+G L+VWAGPLS  
Sbjct: 272 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 331

Query: 358 RLAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCK 417
           R+AVILWNR S ++ IT  W  +GL     V+ RDLW+H   +        A V+PH CK
Sbjct: 332 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYS-CVKKQLSALVEPHACK 391

Query: 418 MFVFT 422
           M+  T
Sbjct: 392 MYTLT 392

BLAST of HG10014727 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 4.2e-141
Identity = 230/365 (63.01%), Postives = 279/365 (76.44%), Query Frame = 0

Query: 59  QNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDS 118
           +NGL RTPQMGWNSWN F C INE +I+ETADALV++GLA+LGY YVNIDDCW    RDS
Sbjct: 57  ENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDS 116

Query: 119 KDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVR-AGSLYHENDDAQLFAS 178
           +   VP+ + FPSGIK +ADYVH+KGLKLGIYSDAG  TC  +  GSL HE  D + FAS
Sbjct: 117 QGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDVKTFAS 176

Query: 179 WGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWR 238
           WG+DYLKYDNC + G   +ER+  M +A+   G++IF+S+CEWG ++PA WAG++GNSWR
Sbjct: 177 WGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWR 236

Query: 239 TTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMK 298
           TT DI D W SMT+ AD N++WAAYAGPGGWNDPDMLEVGNGGM+  EYR+HFS+WAL K
Sbjct: 237 TTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAK 296

Query: 299 SPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSR 358
           +PLLIGCDVR+M+++T  ILSN EVIAVNQD LGVQG+KV+    D  L+VWAGPLS +R
Sbjct: 297 APLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQ---SDNGLEVWAGPLSNNR 356

Query: 359 LAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKM 418
            AV+LWNR S  +TIT  W+ +GL    +V+ RDLW H   A  A     A V PHDCKM
Sbjct: 357 KAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFA--AQGQISASVAPHDCKM 416

Query: 419 FVFTP 423
           +V TP
Sbjct: 417 YVLTP 416

BLAST of HG10014727 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 4.6e-140
Identity = 233/366 (63.66%), Postives = 274/366 (74.86%), Query Frame = 0

Query: 58  LQNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRD 117
           L NGL  TP MGWNSWN F C+++E LI+ETADA+VS GLA LGY Y+N+DDCW    RD
Sbjct: 16  LANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAELNRD 75

Query: 118 SKDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTC-QVRAGSLYHENDDAQLFA 177
           S+  +VP    FPSGIK +ADYVHSKGLKLGIYSDAG  TC +   GSL HE  DA+ FA
Sbjct: 76  SQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDAKTFA 135

Query: 178 SWGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSW 237
           SWG+DYLKYDNC N  I P ER+P M  AL  +GRSIF+S+CEWG +DPA WA +VGNSW
Sbjct: 136 SWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEVGNSW 195

Query: 238 RTTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALM 297
           RTT DI+D+W+SMT+ AD+N+KWA+YAGPGGWNDPDMLEVGNGGMT  EYR+HFS+WAL 
Sbjct: 196 RTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSIWALA 255

Query: 298 KSPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGS 357
           K+PLLIGCD+R+M   T ++LSN EVIAVNQD LGVQG KVK +G    L+VWAGPLSG 
Sbjct: 256 KAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGD---LEVWAGPLSGK 315

Query: 358 RLAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCK 417
           R+AV LWNR S  +TIT  W+ +GL     V+ RDLW H            A VD HD K
Sbjct: 316 RVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAH-STEKSVKGQISAAVDAHDSK 375

Query: 418 MFVFTP 423
           M+V TP
Sbjct: 376 MYVLTP 377

BLAST of HG10014727 vs. ExPASy TrEMBL
Match: A0A0A0KM98 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G220910 PE=3 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 2.0e-247
Identity = 404/430 (93.95%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL LSAA +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLLSAAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDINETLIKETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKP+ADYVHSK LKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWG+
Sbjct: 121 QLVPDPKGFPSGIKPLADYVHSKDLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNC+NLGIKPI+R+PPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCFNLGIKPIKRYPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLAD+NNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADLNNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWNALGLKP+TSVSVRDLW HED+AGDA++SFGAEVDPHDCKMFVF
Sbjct: 361 VLWNRCSVASTITTDWNALGLKPNTSVSVRDLWLHEDVAGDAMSSFGAEVDPHDCKMFVF 420

Query: 421 TPVATSRAEM 431
           TPVAT RAEM
Sbjct: 421 TPVATFRAEM 430

BLAST of HG10014727 vs. ExPASy TrEMBL
Match: G8I091 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=GAL3 PE=2 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.7e-247
Identity = 403/430 (93.72%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL LSAA +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLLSAAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDINETLIKETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKP+ADYVHSK LKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWG+
Sbjct: 121 QLVPDPKGFPSGIKPLADYVHSKDLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNC+NLGIKPI+R+PPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCFNLGIKPIKRYPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLAD+NNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADLNNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVKVFGKDGCLQ+WAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQIWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWNALGLKP+TSVSVRDLW HED+AGDA++SFGAEVDPHDCKMFVF
Sbjct: 361 VLWNRCSVASTITTDWNALGLKPNTSVSVRDLWLHEDVAGDAMSSFGAEVDPHDCKMFVF 420

Query: 421 TPVATSRAEM 431
           TPVAT RAEM
Sbjct: 421 TPVATFRAEM 430

BLAST of HG10014727 vs. ExPASy TrEMBL
Match: A0A1S3C4C2 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103496737 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 5.9e-247
Identity = 405/430 (94.19%), Postives = 418/430 (97.21%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MA R+RF SVFLLIL  S A +AIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MANRIRFGSVFLLILLHSVAYVAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDIN+TLI+ETADALVS+GLAELGYVYVNIDDCWNTQKRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDINDTLIQETADALVSTGLAELGYVYVNIDDCWNTQKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPK FPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI
Sbjct: 121 QLVPDPKGFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPI+RFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIKRFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVRNMTKETSEIL NKEVIAVNQDPLGVQGRKVK FGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRNMTKETSEILMNKEVIAVNQDPLGVQGRKVKDFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN LGLKP+TSVSVRDLW HED+ GDAV+SFGAEVDPHDCKMF+F
Sbjct: 361 VLWNRCSVASTITTDWNVLGLKPNTSVSVRDLWLHEDVEGDAVSSFGAEVDPHDCKMFIF 420

Query: 421 TPVATSRAEM 431
           TPVATSRAEM
Sbjct: 421 TPVATSRAEM 430

BLAST of HG10014727 vs. ExPASy TrEMBL
Match: A0A6J1J4C9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111483287 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 2.6e-242
Identity = 395/430 (91.86%), Postives = 417/430 (96.98%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MAKR+   SVFLLILSL+AAS A+AER VSLLNGYD+SIFKSSFHRIFDTSKYGILQL+N
Sbjct: 1   MAKRISSGSVFLLILSLTAASAAVAERGVSLLNGYDRSIFKSSFHRIFDTSKYGILQLEN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDI+E+LIKETADALVSSGLA+LGYVYVNIDDCW+T KRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDIDESLIKETADALVSSGLADLGYVYVNIDDCWSTLKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPKAFPSGIK VADYVHSKGLKLGIYSDAG+FTCQVR GSLYHENDDA+LFASWG+
Sbjct: 121 QLVPDPKAFPSGIKAVADYVHSKGLKLGIYSDAGIFTCQVRPGSLYHENDDAELFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVR+MTKET EILSN+EVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRSMTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN LGLKP TSVSVRDLW H+D+AGDAVASFGAEVDPHDCKMF+F
Sbjct: 361 VLWNRCSVASTITTDWNTLGLKPGTSVSVRDLWLHKDVAGDAVASFGAEVDPHDCKMFIF 420

Query: 421 TPVATSRAEM 431
           TP AT+RAEM
Sbjct: 421 TPAATARAEM 430

BLAST of HG10014727 vs. ExPASy TrEMBL
Match: A0A6J1FQU5 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447942 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.7e-241
Identity = 394/430 (91.63%), Postives = 415/430 (96.51%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQN 60
           MAKR+   SVFLLILSL+AAS A+AER VSLLNGYD+SIFKSSFHRIFDTSKYGILQLQN
Sbjct: 1   MAKRISSGSVFLLILSLTAASAAVAERGVSLLNGYDRSIFKSSFHRIFDTSKYGILQLQN 60

Query: 61  GLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKD 120
           GLARTPQMGWNSWNFFACDI+E+LIKETADALVSSGLA+LGYVYVNIDDCW+  KRDSKD
Sbjct: 61  GLARTPQMGWNSWNFFACDIDESLIKETADALVSSGLADLGYVYVNIDDCWSALKRDSKD 120

Query: 121 QMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGI 180
           Q+VPDPKAFPSGIK VADYVHSKGLKLGIYSDAG+FTCQVR GSLYHENDDA+LFASWG+
Sbjct: 121 QLVPDPKAFPSGIKTVADYVHSKGLKLGIYSDAGIFTCQVRPGSLYHENDDAELFASWGV 180

Query: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTD 240
           DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYS+CEWGVDDPALWAGKVGNSWRTTD
Sbjct: 181 DYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSLCEWGVDDPALWAGKVGNSWRTTD 240

Query: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPL 300
           DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFS+WALMKSPL
Sbjct: 241 DINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSIWALMKSPL 300

Query: 301 LIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360
           LIGCDVR+MTKET EILSN+EVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV
Sbjct: 301 LIGCDVRSMTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAV 360

Query: 361 ILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVF 420
           +LWNRCSVASTITTDWN LGLKP TSVSVRDLW H+D+AGDAVASFGA+VD HDCKMF+F
Sbjct: 361 VLWNRCSVASTITTDWNTLGLKPGTSVSVRDLWLHKDVAGDAVASFGADVDAHDCKMFIF 420

Query: 421 TPVATSRAEM 431
           TP ATSRAEM
Sbjct: 421 TPAATSRAEM 430

BLAST of HG10014727 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 669.8 bits (1727), Expect = 1.4e-192
Identity = 302/429 (70.40%), Postives = 359/429 (83.68%), Query Frame = 0

Query: 8   ESVFLLILSLSAASLAIAE------RRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQNG 67
           +SV  L++ L + S+ +++      +   L +     +F  SF+ I+DTS YG LQL NG
Sbjct: 9   DSVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNG 68

Query: 68  LARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKDQ 127
           LARTPQMGWNSWNFFAC+INET+IKETADALVSSGLA+LGY++VNIDDCW+   RDS+ Q
Sbjct: 69  LARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRDSEGQ 128

Query: 128 MVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGID 187
           +VP P+ FPSGIK +ADYVHSKGLKLGIYSDAG+FTC+V  GSL+HE DDA +FASWG+D
Sbjct: 129 LVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTCEVHPGSLFHEVDDADIFASWGVD 188

Query: 188 YLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTDD 247
           YLKYDNC+NLGIKPIER+PPMRDALNATGRSIFYS+CEWGVDDPALWA +VGNSWRTTDD
Sbjct: 189 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 248

Query: 248 INDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPLL 307
           INDTWASMTT+AD+NNKWAAYAGPGGWNDPDMLE+GNGGMTY+EYR HFS+WALMK+PLL
Sbjct: 249 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 308

Query: 308 IGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVI 367
           IGCDVRNMT ET EILSNKE+IAVNQDPLGVQGRK++  G++ C QVW+GPLSG R+ V 
Sbjct: 309 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 368

Query: 368 LWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVFT 427
           LWNRCS  +TIT  W+ +GL+   SVSVRDLWQH+D+  +   SF A+VD HDC M+V T
Sbjct: 369 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 428

Query: 428 PVATSRAEM 431
           P   S +++
Sbjct: 429 PQTVSHSDV 437

BLAST of HG10014727 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 623.2 bits (1606), Expect = 1.5e-178
Identity = 288/429 (67.13%), Postives = 341/429 (79.49%), Query Frame = 0

Query: 8   ESVFLLILSLSAASLAIAE------RRVSLLNGYDKSIFKSSFHRIFDTSKYGILQLQNG 67
           +SV  L++ L + S+ +++      +   L +     +F  SF+ I+DTS YG LQL NG
Sbjct: 9   DSVLFLVVGLFSLSVLVSQSIAGRVKAPLLQSNTGGLVFSKSFNSIYDTSMYGRLQLNNG 68

Query: 68  LARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSKDQ 127
           LARTPQMGWNSWNFFAC+INET+IKETADALVSSGLA+LGY++VNI              
Sbjct: 69  LARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI-------------- 128

Query: 128 MVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTCQVRAGSLYHENDDAQLFASWGID 187
                     GIK +ADYVHSKGLKLGIYSDAG+FTC+V  GSL+HE DDA +FASWG+D
Sbjct: 129 ----------GIKLLADYVHSKGLKLGIYSDAGVFTCEVHPGSLFHEVDDADIFASWGVD 188

Query: 188 YLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRTTDD 247
           YLKYDNC+NLGIKPIER+PPMRDALNATGRSIFYS+CEWGVDDPALWA +VGNSWRTTDD
Sbjct: 189 YLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSWRTTDD 248

Query: 248 INDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKSPLL 307
           INDTWASMTT+AD+NNKWAAYAGPGGWNDPDMLE+GNGGMTY+EYR HFS+WALMK+PLL
Sbjct: 249 INDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALMKAPLL 308

Query: 308 IGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVI 367
           IGCDVRNMT ET EILSNKE+IAVNQDPLGVQGRK++  G++ C QVW+GPLSG R+ V 
Sbjct: 309 IGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVA 368

Query: 368 LWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMFVFT 427
           LWNRCS  +TIT  W+ +GL+   SVSVRDLWQH+D+  +   SF A+VD HDC M+V T
Sbjct: 369 LWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLT 413

Query: 428 PVATSRAEM 431
           P   S +++
Sbjct: 429 PQTVSHSDV 413

BLAST of HG10014727 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 505.4 bits (1300), Expect = 4.6e-143
Identity = 233/365 (63.84%), Postives = 276/365 (75.62%), Query Frame = 0

Query: 58  LQNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRD 117
           + NGLA +PQMGWNSWN F C+INETLIK+TADA+VSSGL+ +GY Y+NIDDCW   KRD
Sbjct: 32  MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 91

Query: 118 SKDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTC-QVRAGSLYHENDDAQLFA 177
           S+  +V     FPSGIK ++DYVHSKGLKLGIYSDAG  TC Q   GSL HE  DA+ FA
Sbjct: 92  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 151

Query: 178 SWGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSW 237
           SWGIDYLKYDNC N G  P ER+P M  AL  +GRSIF+S+CEWG +DPA WAG +GNSW
Sbjct: 152 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 211

Query: 238 RTTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALM 297
           RTT DI D W SMT +AD N++WA+YA PG WNDPDMLEVGNGGMT +EY +HFS+WAL 
Sbjct: 212 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 271

Query: 298 KSPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGS 357
           K+PLLIGCD+R+M K T E+LSNKEVIAVNQD LG+QG+KVK   K+G L+VWAGPLS  
Sbjct: 272 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 331

Query: 358 RLAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCK 417
           R+AVILWNR S ++ IT  W  +GL     V+ RDLW+H   +        A V+PH CK
Sbjct: 332 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYS-CVKKQLSALVEPHACK 391

Query: 418 MFVFT 422
           M+  T
Sbjct: 392 MYTLT 392

BLAST of HG10014727 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 505.4 bits (1300), Expect = 4.6e-143
Identity = 233/365 (63.84%), Postives = 276/365 (75.62%), Query Frame = 0

Query: 58  LQNGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRD 117
           + NGLA +PQMGWNSWN F C+INETLIK+TADA+VSSGL+ +GY Y+NIDDCW   KRD
Sbjct: 6   MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 65

Query: 118 SKDQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTC-QVRAGSLYHENDDAQLFA 177
           S+  +V     FPSGIK ++DYVHSKGLKLGIYSDAG  TC Q   GSL HE  DA+ FA
Sbjct: 66  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 125

Query: 178 SWGIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSW 237
           SWGIDYLKYDNC N G  P ER+P M  AL  +GRSIF+S+CEWG +DPA WAG +GNSW
Sbjct: 126 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 185

Query: 238 RTTDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALM 297
           RTT DI D W SMT +AD N++WA+YA PG WNDPDMLEVGNGGMT +EY +HFS+WAL 
Sbjct: 186 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 245

Query: 298 KSPLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGS 357
           K+PLLIGCD+R+M K T E+LSNKEVIAVNQD LG+QG+KVK   K+G L+VWAGPLS  
Sbjct: 246 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 305

Query: 358 RLAVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCK 417
           R+AVILWNR S ++ IT  W  +GL     V+ RDLW+H   +        A V+PH CK
Sbjct: 306 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYS-CVKKQLSALVEPHACK 365

Query: 418 MFVFT 422
           M+  T
Sbjct: 366 MYTLT 366

BLAST of HG10014727 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 483.4 bits (1243), Expect = 1.9e-136
Identity = 235/426 (55.16%), Postives = 299/426 (70.19%), Query Frame = 0

Query: 1   MAKRVRFESVFLLILSLSAASLAIAERRVSLLNGYDKS-IFKSSFHRIFDTSKYGILQLQ 60
           M++R     + +L++ +S+  + + E   S+ NG+D S I +                L 
Sbjct: 1   MSRRAMVIKMPILMILISSMVMTMVESSRSVNNGHDDSEILRRHL-------------LT 60

Query: 61  NGLARTPQMGWNSWNFFACDINETLIKETADALVSSGLAELGYVYVNIDDCWNTQKRDSK 120
           NGL  TP MGWNSWN F+C+I+E +IKETADALV++GL++LGY YVNIDDCW    RDSK
Sbjct: 61  NGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSK 120

Query: 121 DQMVPDPKAFPSGIKPVADYVHSKGLKLGIYSDAGLFTC-QVRAGSLYHENDDAQLFASW 180
             +VP    FPSGIK VADYVHSKGLKLGIYSDAG FTC +   GSL +E  DA+ FA W
Sbjct: 121 GSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEW 180

Query: 181 GIDYLKYDNCYNLGIKPIERFPPMRDALNATGRSIFYSICEWGVDDPALWAGKVGNSWRT 240
           GIDYLKYDNC + G KP  R+P M  AL  +GR IF+S+CEWG   PALW   VGNSWRT
Sbjct: 181 GIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRT 240

Query: 241 TDDINDTWASMTTLADINNKWAAYAGPGGWNDPDMLEVGNGGMTYQEYRAHFSVWALMKS 300
           T+DI DTW SM ++AD+N  +A +A PGGWNDPDMLEVGNGGMT  EY  HFS+WA+ K+
Sbjct: 241 TNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKA 300

Query: 301 PLLIGCDVRNMTKETSEILSNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRL 360
           PLL+GCD+RNMTKET EI++NKEVIA+NQDP GVQ +KV++   +G L+VWAGPLSG R+
Sbjct: 301 PLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRM---EGDLEVWAGPLSGYRV 360

Query: 361 AVILWNRCSVASTITTDWNALGLKPDTSVSVRDLWQHEDIAGDAVASFGAEVDPHDCKMF 420
           A++L NR    ++IT  W  + +  ++ V  RDLW+H+ +    V +  A VD H CK++
Sbjct: 361 ALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLY 410

Query: 421 VFTPVA 425
           V  PVA
Sbjct: 421 VLKPVA 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892272.12.1e-25196.28alpha-galactosidase 3 [Benincasa hispida][more]
KGN50728.14.2e-24793.95hypothetical protein Csa_005887 [Cucumis sativus][more]
NP_001292680.15.5e-24793.72alpha-galactosidase 3 precursor [Cucumis sativus] >AEQ94270.1 alpha galacdosidas... [more]
XP_008456938.11.2e-24694.19PREDICTED: alpha-galactosidase 3 isoform X1 [Cucumis melo][more]
XP_022985233.15.3e-24291.86alpha-galactosidase 3 [Cucurbita maxima] >XP_022985242.1 alpha-galactosidase 3 [... [more]
Match NameE-valueIdentityDescription
Q8VXZ72.0e-19170.40Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1[more]
P147495.3e-14463.04Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q8RX866.5e-14263.84Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
Q9FXT44.2e-14163.01Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Q426564.6e-14063.66Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KM982.0e-24793.95Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G220910 PE=3 SV=1[more]
G8I0912.7e-24793.72Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=GAL3 PE=2 SV=1[more]
A0A1S3C4C25.9e-24794.19Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103496737 PE=3 SV=1[more]
A0A6J1J4C92.6e-24291.86Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111483287 PE=3 SV=1[more]
A0A6J1FQU53.7e-24191.63Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447942 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G56310.11.4e-19270.40Melibiase family protein [more]
AT3G56310.21.5e-17867.13Melibiase family protein [more]
AT5G08370.14.6e-14363.84alpha-galactosidase 2 [more]
AT5G08370.24.6e-14363.84alpha-galactosidase 2 [more]
AT5G08380.11.9e-13655.16alpha-galactosidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 96..111
score: 66.88
coord: 60..79
score: 66.5
coord: 198..216
score: 58.42
coord: 284..305
score: 56.82
coord: 263..282
score: 73.75
coord: 137..158
score: 63.18
coord: 171..188
score: 74.72
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 65..328
e-value: 6.5E-81
score: 271.6
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 42..421
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 66..328
e-value: 2.01535E-157
score: 443.535
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 58..330
e-value: 1.0E-131
score: 440.5
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 331..423
e-value: 4.8E-28
score: 99.0
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 343..419
e-value: 7.8E-20
score: 70.7
NoneNo IPR availablePANTHERPTHR11452:SF75ALPHA-GALACTOSIDASE 3coord: 42..421
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 323..421
IPR000111Glycoside hydrolase family 27/36, conserved sitePROSITEPS00512ALPHA_GALACTOSIDASEcoord: 101..117
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 57..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10014727.1HG10014727.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009911 positive regulation of flower development
biological_process GO:0009620 response to fungus
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds