HG10014336 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014336 vs. NCBI nr
Match: XP_011654227.1 (phospholipase D Y isoform X1 [Cucumis sativus] >KGN55427.1 hypothetical protein Csa_012518 [Cucumis sativus])

HSP 1 Score: 798.1 bits (2060), Expect = 4.1e-227
Identity = 410/521 (78.69%), Postives = 437/521 (83.88%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL--FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLST 60
           M KAQT T+TTIFSAL  FFF+SQF PVLSFTQ QCK WLVQSIPTDMPQLQ VQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYS+EDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDK 180
           AADRNVNVRLVSHSGV PNYGKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD+
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH-- 240
           DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++   
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLA-FLNRTDYTRTIFDH 240

Query: 241 -----------TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG-- 300
                      +  I+P+S                  P I+   +L LS+  P       
Sbjct: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300

Query: 301 -----------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
                      P+LLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS
Sbjct: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360

Query: 361 SLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDCFGKVEIK
Sbjct: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420

Query: 421 YYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 478
           YYVVPGYNETGPAL+ NGTRTKN YP F+RVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG
Sbjct: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480

BLAST of HG10014336 vs. NCBI nr
Match: XP_038900272.1 (phospholipase D Y-like [Benincasa hispida])

HSP 1 Score: 795.8 bits (2054), Expect = 2.0e-226
Identity = 411/522 (78.74%), Postives = 437/522 (83.72%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           MMKAQT TVTTIFSAL   FFFISQF PVLSFT+ QCKAWLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MMKAQTPTVTTIFSALFFFFFFISQFPPVLSFTRSQCKAWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST+RLDIIAQYWELL GP+DSRSGDYGYS EDLKKFGA+KGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTQRLDIIAQYWELLVGPNDSRSGDYGYSIEDLKKFGASKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYPNY KE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPNYVKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSL-- 240
           +DVYIGSANQDWKSLSQVKELGIYL GCPR+AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLAGCPRIAAHVKVYFDNLLKLA-FLNHTDYTRTIFD 240

Query: 241 -----HTQI------INPDS----------------WEPLINVAASLLLSLFRPSREFG- 300
                H ++      I+P+S                  P I+   +L LS+  P      
Sbjct: 241 HQWQTHRKVPCWSYFIDPESRCRSPLPPYMKFLHTLGYPFISDPYTLKLSIQTPGSSLST 300

Query: 301 ------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYW 360
                       P+LLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQS+FTTPTIYW
Sbjct: 301 LLPYSCYVSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSQFTTPTIYW 360

Query: 361 SSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEI 420
           SSLSSAISEVVFSKNATVKLLVSYWTHFVE TD YLKSLLYSNILCNSSKFNDCFGKVEI
Sbjct: 361 SSLSSAISEVVFSKNATVKLLVSYWTHFVESTDPYLKSLLYSNILCNSSKFNDCFGKVEI 420

Query: 421 KYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTS 478
           KYYVVPGYNETGPAL+ NGTRTKN YPSFTRVQHGKF VSDVRAHIGTSNLGWDYFYVTS
Sbjct: 421 KYYVVPGYNETGPALYHNGTRTKNAYPSFTRVQHGKFVVSDVRAHIGTSNLGWDYFYVTS 480

BLAST of HG10014336 vs. NCBI nr
Match: XP_008452848.1 (PREDICTED: phospholipase D Z-like [Cucumis melo] >TYK20010.1 phospholipase D Z-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.3 bits (2045), Expect = 2.2e-225
Identity = 406/522 (77.78%), Postives = 436/522 (83.52%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           M K  +LTVTTIFSAL   FFFISQF PVLSFT+ QCK WLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYSNEDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYP +GKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH- 240
           +DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLA-FLNPTDYTRTIFD 240

Query: 241 ------------TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG- 300
                       +  I+P+S                  P+I+   +L LS+  P      
Sbjct: 241 HQWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPIISDPYTLNLSIQTPGSRLST 300

Query: 301 ------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYW 360
                       P+LLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT PTIYW
Sbjct: 301 LLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFTNPTIYW 360

Query: 361 SSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEI 420
           SSLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDCFGKVEI
Sbjct: 361 SSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEI 420

Query: 421 KYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTS 478
           KYYVVPGYNETGPAL+ N TRT+N YPSFTRVQHGK+AVSDVRAHIGTSNLGWDYFYVTS
Sbjct: 421 KYYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWDYFYVTS 480

BLAST of HG10014336 vs. NCBI nr
Match: KAA0064580.1 (phospholipase D Z-like [Cucumis melo var. makuwa])

HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 406/528 (76.89%), Postives = 436/528 (82.58%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           M K  +LTVTTIFSAL   FFFISQF PVLSFT+ QCK WLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYSNEDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYP +GKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH- 240
           +DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLA-FLNPTDYTRTIFD 240

Query: 241 ------------TQIINPDS----------------------WEPLINVAASLLLSLFRP 300
                       +  I+P+S                        P+I+   +L LS+  P
Sbjct: 241 HQWQTQRKVPCWSYFIDPESRCRQVQPFSSPLPPYVKFLHTLGYPIISDPYTLNLSIQTP 300

Query: 301 SREFG-------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFT 360
                             P+LLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT
Sbjct: 301 GSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFT 360

Query: 361 TPTIYWSSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDC 420
            PTIYWSSLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDC
Sbjct: 361 NPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDC 420

Query: 421 FGKVEIKYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWD 478
           FGKVEIKYYVVPGYNETGPAL+ N TRT+N YPSFTRVQHGK+AVSDVRAHIGTSNLGWD
Sbjct: 421 FGKVEIKYYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWD 480

BLAST of HG10014336 vs. NCBI nr
Match: KAG6591527.1 (Phospholipase D Y, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024415.1 Phospholipase D Y [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785.4 bits (2027), Expect = 2.7e-223
Identity = 399/514 (77.63%), Postives = 432/514 (84.05%), Query Frame = 0

Query: 6   TLTVTTIFSALFFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL 65
           T+TVTTIFSALFFF SQF PVLSF+QPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL
Sbjct: 7   TVTVTTIFSALFFFTSQFPPVLSFSQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL 66

Query: 66  AGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIEKAADRNVN 125
           AGNST+RLDIIAQYW+LLA PDDSRSG+YGYS+EDLKKFGA +GYDVYSAIEKAADR+VN
Sbjct: 67  AGNSTQRLDIIAQYWDLLAAPDDSRSGEYGYSDEDLKKFGAYQGYDVYSAIEKAADRHVN 126

Query: 126 VRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDKDVYIGSA 185
           VRLVSHSGVYPNYGKE ADLASGRPNVQ+VTLLLGDWYGSGIVH+KVWISDDKDVYIGSA
Sbjct: 127 VRLVSHSGVYPNYGKEPADLASGRPNVQDVTLLLGDWYGSGIVHTKVWISDDKDVYIGSA 186

Query: 186 NQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH--------- 245
           NQDWKSLSQVKELGIYLTGCP +AAHVKV    + ++S ++  + Y R++          
Sbjct: 187 NQDWKSLSQVKELGIYLTGCPSIAAHVKVYFDNLLKLS-FLNHTDYTRTVFDHQWQIQRK 246

Query: 246 ----TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG--------- 305
               +  I+P+S                  P I+  ++L LS+  P   F          
Sbjct: 247 VPCWSYFIDPESRCRSPLPPYVNFPHTLGYPFISDPSTLNLSIQTPGSSFSTLLPHSCYL 306

Query: 306 ----PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAIS 365
               P+LLFGRYQSDEQAWSDTIKSVSSG TVRISTMDWLGQSEFTTPTI+WSSLSSAIS
Sbjct: 307 SFAPPELLFGRYQSDEQAWSDTIKSVSSGETVRISTMDWLGQSEFTTPTIFWSSLSSAIS 366

Query: 366 EVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGY 425
           EV+FSKNATVKLLVSYWTHFVE TD YLKSLLYSNILCNSSKFN CFGKVEIKYYVVPGY
Sbjct: 367 EVIFSKNATVKLLVSYWTHFVENTDPYLKSLLYSNILCNSSKFNHCFGKVEIKYYVVPGY 426

Query: 426 NETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYN 478
           N TGPAL+ NGTRT NTYPSFTRVQHGKFAVS+VRAHIGTSNL WDYFY TSGVSFGTYN
Sbjct: 427 NRTGPALYPNGTRTNNTYPSFTRVQHGKFAVSNVRAHIGTSNLAWDYFYATSGVSFGTYN 486

BLAST of HG10014336 vs. ExPASy Swiss-Prot
Match: Q54SA1 (Phospholipase D Z OS=Dictyostelium discoideum OX=44689 GN=pldZ PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.7e-43
Identity = 126/452 (27.88%), Postives = 203/452 (44.91%), Query Frame = 0

Query: 27  LSFTQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWLAGNSTERLDIIAQYWELLAGP 86
           LSF Q  C    +Q I   +P    +Q  LST D ++ L  N+ + +D+   Y  L  G 
Sbjct: 27  LSF-QSNCLGGSIQ-IAESIPLALNLQSNLSTHDAWMELITNAKKSIDMGIFYMTLTDG- 86

Query: 87  DDSRSGDYGYSNEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVYPNYGKEAADLA 146
                       +    +G   G D+Y A+  A  R V++R+V +         +  +LA
Sbjct: 87  -----------GQLDPVYGGQLGLDIYKALVDANSRGVSIRIVQNQPSSSMPDTDTQNLA 146

Query: 147 SGRPNVQNVTLLLGDWYGSGIVHSKVWISDDKDVYIGSANQDWKSLSQVKELGIYLTGCP 206
             +  VQ  ++      G+GI+H+KV + D    Y+GSAN DW+SL+QVKELG+    CP
Sbjct: 147 --KLGVQVRSINWPSLVGAGILHTKVIVVDQVSAYLGSANLDWRSLAQVKELGVLFQNCP 206

Query: 207 RVAAHVKVTASTICEVSPYIRISVYIRSLHTQIINPDSWEPLINVA------ASLLLSLF 266
            + +  ++      + +    +             P  W      A      ASLLL+  
Sbjct: 207 SMVSDTEIAFQQYWDAAVVTEL-------------PSDWGVQYQAAYNQTNMASLLLNGN 266

Query: 267 RPSREF----GPQLLFGRYQSDEQAWSDTIKSVSSGA--TVRISTMDWLGQSEFTTPTIY 326
                F     PQ +     +D     D + S  +GA  T+ IS MD++  S + +P  +
Sbjct: 267 EKFEMFLAVSPPQFV----STDRTGDIDALVSAMNGATKTICISVMDYIPASLYNSPNTF 326

Query: 327 WSSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVE 386
           W  + +A+    +++   V++L+S+W H       +L SL             D    ++
Sbjct: 327 WPVMDNALRAAAYNRGVQVRMLISHWNHTNYAIPQWLHSL-------------DQVNNID 386

Query: 387 IKYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVT 446
           ++++VVP +                    FTRV H K+ V+D ++++GTSN   DY+  T
Sbjct: 387 VRWFVVPDF-------------PNEPQVPFTRVNHAKYMVTDEQSYVGTSNWSEDYYTNT 419

Query: 447 SGVSFGTYNPAIVKQLQEVFDADWNSPYTVPV 467
            G+S+  YN     QLQ +FD DWNSPY+ PV
Sbjct: 447 GGLSYNIYNDEFTSQLQSIFDRDWNSPYSFPV 419

BLAST of HG10014336 vs. ExPASy Swiss-Prot
Match: Q54K50 (Phospholipase D Y OS=Dictyostelium discoideum OX=44689 GN=pldY PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.7e-42
Identity = 127/475 (26.74%), Postives = 214/475 (45.05%), Query Frame = 0

Query: 12  IFSALFFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWLAGNSTE 71
           I   LFF       +   T   C+   +Q I   +P    +   LST D ++ L  N+ E
Sbjct: 8   IIIVLFFVNVNSKIISESTLNSCQGGTIQ-IAESIPLGLDISTNLSTHDAWMDLITNAQE 67

Query: 72  RLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSH 131
            +D+   Y+ LL G D                +G   G D+++AI +A  R +NVR+V +
Sbjct: 68  SIDLGFFYFTLLGGSDLD------------PVYGGQLGIDIFNAIVEAHSRGINVRIVQN 127

Query: 132 SGVYPNYGKEAADLASGRPNVQNVTLLLGDW---YGSGIVHSKVWISDDKDVYIGSANQD 191
                    E   LA     V+++     DW    GSG++H+K+ I D+   Y+GSAN D
Sbjct: 128 EPSESFPDTETQTLAKLGIQVRSI-----DWVSLVGSGVLHTKLIIIDESSAYVGSANAD 187

Query: 192 WKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLHTQII-------- 251
           W SL+QVKELGI L  CP + A  ++        + +  + +     + Q +        
Sbjct: 188 WSSLAQVKELGIVLKNCPTMVADTEIAFQQYWNAADFTSLPINDWGSNYQALFNNTNMAS 247

Query: 252 -------NPDSWEPLINVAASLLLSLFRPSREFGPQLLFGRYQS-DEQAWSDTIKSVSSG 311
                  N ++     +   S    ++       P      Y++ D  A  D I +    
Sbjct: 248 LSLNNNNNNNNKNKNNDNDGSGTNQMYEMFLAVSPPQFQSTYRTGDIDALMDAINNADQ- 307

Query: 312 ATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLK 371
            ++ I+ MD+   + +  P  YW  + +A+    F++N  V++L+S+W +       +L 
Sbjct: 308 -SICITVMDYTPTTLYNDPNTYWPLIDNALRAAAFNRNVQVRMLISHWNYTSPIIPQWLH 367

Query: 372 SLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKF 431
           SL             +    ++++++VVP ++ T P +             +TRV H KF
Sbjct: 368 SL-------------NQVDNIQVRWFVVPDFS-TNPQI------------PYTRVNHAKF 427

Query: 432 AVSDVRAHIGTSNLGWDYFYVTSGVSFGTYNPAIVKQLQEVFDADWNSPYTVPVK 468
            V++ ++++GTSN   DYF VT G+S+  +N     QLQ +F+ DWNSPYT P++
Sbjct: 428 MVTEKQSYVGTSNWSQDYFTVTGGLSYNVFNNDFTNQLQSIFNRDWNSPYTQPIQ 436

BLAST of HG10014336 vs. ExPASy Swiss-Prot
Match: O17405 (Probable phospholipase D F09G2.8 OS=Caenorhabditis elegans OX=6239 GN=F09G2.8 PE=1 SV=2)

HSP 1 Score: 165.6 bits (418), Expect = 1.4e-39
Identity = 138/445 (31.01%), Postives = 209/445 (46.97%), Query Frame = 0

Query: 34  CKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGD 93
           CK +LV+SIP  +P     +    T + ++ +  NS + LDI   YW L     D +S  
Sbjct: 163 CKTFLVESIPIGLP----FKTNNHTAEAWINIIDNSKQYLDISVMYWNL--NTSDYKSSV 222

Query: 94  YGYSNEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVYPNYGKEAADLA-SGRPNV 153
           YG                VY AI +A  R V +R+        +  KE+A L   G   V
Sbjct: 223 YGRR--------------VYEAIIRAGKRGVKIRIAQDGASNLSDNKESAYLVQEGLAEV 282

Query: 154 Q--NVTLLLGDWYGSGIVHSKVWISDDKDVYIGSANQDWKSLSQVKELGIYLTGCPRVAA 213
           +  NVT L+    GSGI+H+K  +SD   +YIGSAN DWKSLS+VKE+G+    CP VA+
Sbjct: 283 REINVTRLI----GSGIIHTKFILSDIATLYIGSANMDWKSLSEVKEVGVVFQECPCVAS 342

Query: 214 HVKVTASTICEVSPYIRISVYIRSLHTQIINPDSW----EPLINVAASLLLSLFRPSREF 273
            +            Y   + Y +      + P+ W        N ++   L++     E+
Sbjct: 343 DL------------YKIFAAYWKLGENDSVIPEKWPISYRTPFNFSSMAKLTMDGEPAEY 402

Query: 274 ------GPQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPT-IYWSSLS 333
                 GP    GR + D  A    +K      +V IS MD++  + +   +  +W  + 
Sbjct: 403 FISSSPGPFNPKGR-EHDLAAIQKIMKDARK--SVCISVMDYIPSTLYMKKSNRFWPEID 462

Query: 334 SAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDC-FGKVEIKYY 393
            +I +  + +   V++L+S+W H  +    +LKSL    I     ++N    G+V+++ +
Sbjct: 463 DSIRDAAY-RGVNVRMLISHWDHSRKEMIPFLKSL--QTITDGLPRYNRTEHGQVQVRIF 522

Query: 394 VVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVS 453
            VP           NG   K   P FTRV H K+ V++  A+IGTSN   DYF  T+GV+
Sbjct: 523 TVP----------PNG---KEKIP-FTRVNHAKYMVTEDIAYIGTSNWSGDYFISTAGVA 551

Query: 454 FGTYNPAIVKQLQEVFDADWNSPYT 464
                P+  K+LQ VFD DWNS Y+
Sbjct: 583 MVVRQPSATKRLQNVFDRDWNSEYS 551

BLAST of HG10014336 vs. ExPASy Swiss-Prot
Match: Q6PB03 (5'-3' exonuclease PLD3 OS=Xenopus laevis OX=8355 GN=pld3 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.2e-33
Identity = 127/444 (28.60%), Postives = 201/444 (45.27%), Query Frame = 0

Query: 33  QCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSG 92
           QC+  LV+SIP  +          S    ++ +  N+   +DI + YW L          
Sbjct: 81  QCRFVLVESIPEGLVYDANSTINPSIFQSWMNIITNAKSSIDIASFYWSL---------- 140

Query: 93  DYGYSNED--LKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVYPNYGKEAADLASGRP 152
               +NED   K+  A++G  +   +     R V++R+  +    P   K+ + L     
Sbjct: 141 ----TNEDTQTKEPSAHQGELILQELLNLKQRGVSLRVAVNPPDSPIRSKDISALKDRGA 200

Query: 153 NVQNVTLLLGDWYGSGIVHSKVWISDDKDVYIGSANQDWKSLSQVKELGIYLTGCPRVAA 212
           +V+ V +        GI+H+K W+ D++  YIGSAN DW+SL+QVKELG  +  C  +A 
Sbjct: 201 DVRVVDM---PKLTDGILHTKFWVVDNEHFYIGSANMDWRSLTQVKELGATIYNCSCLAQ 260

Query: 213 HVK--VTASTICEVSPYIRISVYIRSLHTQIINPDSWEPLINVAASLLLSLFRPSREFGP 272
            +K    A  I  +      S +  +  T        + ++N  AS +     P     P
Sbjct: 261 DLKKIFEAYWILGLPNATLPSPWPANYSTPYNKDTPMQVMLNSTASQVYLSSSP-----P 320

Query: 273 QLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAISEVVFS 332
            L       D Q+  + I        V IS MD+    EF+ P  YW  + + + + V+ 
Sbjct: 321 PLSATGRTDDLQSIMNIIDDAKK--FVYISVMDYSPTEEFSHPRRYWPEIDNHLRKAVYE 380

Query: 333 KNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGYNETGP 392
           +N  V+LL+S W +      ++L+SL  + +  N+S +N     +E+K +VVP       
Sbjct: 381 RNVNVRLLISCWKNSRPSMFTFLRSL--AALHSNTSHYN-----IEVKIFVVP------- 440

Query: 393 ALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG--------VSFG 452
                 T  +   P + RV H K+ V+D  A+IGTSN   DYF  T+G         S G
Sbjct: 441 -----ATEAQKKIP-YARVNHNKYMVTDRVAYIGTSNWSGDYFINTAGSALVVNQTQSAG 479

Query: 453 TYNPAIVKQLQEVFDADWNSPYTV 465
           T +  I  QLQ VF+ DWNS Y++
Sbjct: 501 T-SDTIQMQLQTVFERDWNSNYSL 479

BLAST of HG10014336 vs. ExPASy Swiss-Prot
Match: Q8BG07 (5'-3' exonuclease PLD4 OS=Mus musculus OX=10090 GN=Pld4 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.4e-31
Identity = 123/450 (27.33%), Postives = 193/450 (42.89%), Query Frame = 0

Query: 34  CKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWLAGNSTERLDIIAQYWELLA---GPDDSR 93
           C+  LV+SIP D+P              ++ L   + E + I + YW L     G +DS 
Sbjct: 92  CQLILVESIPEDLPFAAGSPTAQPLAQAWLQLLDTARESVHIASYYWSLTGLDIGVNDSS 151

Query: 94  SGDYGYSNEDLKKFGANKGYDVYSAIEKAADRNVNVRLVSHSGVYPNYGKEAADLASGRP 153
           S                +G  +    ++   RN++V + +HS   P   K + DL     
Sbjct: 152 S---------------RQGEALLQKFQQLLLRNISVVVATHS---PTLAKTSTDLQVLAA 211

Query: 154 NVQNVTLLLGDWYGSGIVHSKVWISDDKDVYIGSANQDWKSLSQVKELGIYLTGCPRVAA 213
           +   +  +       G++HSK W+ D + +Y+GSAN DW+SL+QVKELG  +  C  +A 
Sbjct: 212 HGAQIRQVPMKQLTGGVLHSKFWVVDGRHIYVGSANMDWRSLTQVKELGAIIYNCSNLAQ 271

Query: 214 HVKVTAST--ICEVSPYIRISVYIR--SLHTQIINPDSWEPLINVAASLLLSLFRPSREF 273
            ++ T  T  +      +    + R  S H    +P    P   V  +   S   PS   
Sbjct: 272 DLEKTFQTYWVLGTPQAVLPKTWPRNFSSHINRFHP-LRGPFDGVPTTAYFSASPPS--- 331

Query: 274 GPQLLFGRYQSDEQAWSDTIKSVSSGAT--VRISTMDWLGQSEFTTPTIYWSSLSSAISE 333
                 GR +       D +  V  GA   + +S M++   + FT    YW  L +A+  
Sbjct: 332 --LCPHGRTRD-----LDAVLGVMEGARQFIYVSVMEYFPTTRFTHHARYWPVLDNALRA 391

Query: 334 VVFSKNATVKLLVSYWTHFVEGTDSYLKSL-LYSNILCNSSKFNDCFGKVEIKYYVVPGY 393
              +K   V+LLVS W +      +YL+SL  +SN     S        V++K ++VP  
Sbjct: 392 AALNKGVHVRLLVSCWFNTDPTMFAYLRSLQAFSNPSAGIS--------VDVKVFIVPVG 451

Query: 394 NETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYN 453
           N      H N          F+RV H KF V+D  A++GTSN   DYF  T+GV      
Sbjct: 452 N------HSN--------IPFSRVNHSKFMVTDKTAYVGTSNWSEDYFSHTAGVGLIVSQ 490

Query: 454 ---------PAIVKQLQEVFDADWNSPYTV 465
                      + +QL+++F+ DW+S Y +
Sbjct: 512 KTPRAQPGATTVQEQLRQLFERDWSSHYAM 490

BLAST of HG10014336 vs. ExPASy TrEMBL
Match: A0A0A0L498 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G651930 PE=4 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 2.0e-227
Identity = 410/521 (78.69%), Postives = 437/521 (83.88%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL--FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLST 60
           M KAQT T+TTIFSAL  FFF+SQF PVLSFTQ QCK WLVQSIPTDMPQLQ VQGVLST
Sbjct: 1   MKKAQTPTLTTIFSALFFFFFVSQFPPVLSFTQSQCKGWLVQSIPTDMPQLQLVQGVLST 60

Query: 61  RDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIEK 120
           RDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYS+EDLKKFGANKGYDVYSAIEK
Sbjct: 61  RDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSDEDLKKFGANKGYDVYSAIEK 120

Query: 121 AADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDK 180
           AADRNVNVRLVSHSGV PNYGKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD+
Sbjct: 121 AADRNVNVRLVSHSGVSPNYGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDR 180

Query: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH-- 240
           DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++   
Sbjct: 181 DVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFENLLKLA-FLNRTDYTRTIFDH 240

Query: 241 -----------TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG-- 300
                      +  I+P+S                  P I+   +L LS+  P       
Sbjct: 241 QWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPTISDPYTLNLSIQTPGSRLSTL 300

Query: 301 -----------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360
                      P+LLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS
Sbjct: 301 LPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWS 360

Query: 361 SLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEIK 420
           SLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDCFGKVEIK
Sbjct: 361 SLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEIK 420

Query: 421 YYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 478
           YYVVPGYNETGPAL+ NGTRTKN YP F+RVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG
Sbjct: 421 YYVVPGYNETGPALYHNGTRTKNEYPGFSRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSG 480

BLAST of HG10014336 vs. ExPASy TrEMBL
Match: A0A5D3D952 (Phospholipase D Z-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001270 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.1e-225
Identity = 406/522 (77.78%), Postives = 436/522 (83.52%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           M K  +LTVTTIFSAL   FFFISQF PVLSFT+ QCK WLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYSNEDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYP +GKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH- 240
           +DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLA-FLNPTDYTRTIFD 240

Query: 241 ------------TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG- 300
                       +  I+P+S                  P+I+   +L LS+  P      
Sbjct: 241 HQWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPIISDPYTLNLSIQTPGSRLST 300

Query: 301 ------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYW 360
                       P+LLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT PTIYW
Sbjct: 301 LLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFTNPTIYW 360

Query: 361 SSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEI 420
           SSLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDCFGKVEI
Sbjct: 361 SSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEI 420

Query: 421 KYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTS 478
           KYYVVPGYNETGPAL+ N TRT+N YPSFTRVQHGK+AVSDVRAHIGTSNLGWDYFYVTS
Sbjct: 421 KYYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWDYFYVTS 480

BLAST of HG10014336 vs. ExPASy TrEMBL
Match: A0A1S3BUV2 (phospholipase D Z-like OS=Cucumis melo OX=3656 GN=LOC103493746 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.1e-225
Identity = 406/522 (77.78%), Postives = 436/522 (83.52%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           M K  +LTVTTIFSAL   FFFISQF PVLSFT+ QCK WLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYSNEDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYP +GKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH- 240
           +DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLA-FLNPTDYTRTIFD 240

Query: 241 ------------TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG- 300
                       +  I+P+S                  P+I+   +L LS+  P      
Sbjct: 241 HQWQTQRKVPCWSYFIDPESRCRSPLPPYVKFLHTLGYPIISDPYTLNLSIQTPGSRLST 300

Query: 301 ------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYW 360
                       P+LLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT PTIYW
Sbjct: 301 LLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFTNPTIYW 360

Query: 361 SSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEI 420
           SSLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDCFGKVEI
Sbjct: 361 SSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDCFGKVEI 420

Query: 421 KYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTS 478
           KYYVVPGYNETGPAL+ N TRT+N YPSFTRVQHGK+AVSDVRAHIGTSNLGWDYFYVTS
Sbjct: 421 KYYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWDYFYVTS 480

BLAST of HG10014336 vs. ExPASy TrEMBL
Match: A0A5A7VBH6 (Phospholipase D Z-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003020 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.4e-225
Identity = 406/528 (76.89%), Postives = 436/528 (82.58%), Query Frame = 0

Query: 1   MMKAQTLTVTTIFSAL---FFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLS 60
           M K  +LTVTTIFSAL   FFFISQF PVLSFT+ QCK WLVQSIPTDMPQLQ VQGVLS
Sbjct: 1   MKKTHSLTVTTIFSALLFFFFFISQFPPVLSFTRSQCKGWLVQSIPTDMPQLQLVQGVLS 60

Query: 61  TRDVFVWLAGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120
           TRDVFVWLAGNST RLDIIAQYWELLAGP+DSRSGDYGYSNEDLKKFGANKGYDVYSAIE
Sbjct: 61  TRDVFVWLAGNSTRRLDIIAQYWELLAGPNDSRSGDYGYSNEDLKKFGANKGYDVYSAIE 120

Query: 121 KAADRNVNVRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180
           KAADRNVNVRLVSHSGVYP +GKE ADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD
Sbjct: 121 KAADRNVNVRLVSHSGVYPTFGKEPADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDD 180

Query: 181 KDVYIGSANQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH- 240
           +DVYIGSANQDWKSLSQVKELGIYLTGCP++AAHVKV    + +++ ++  + Y R++  
Sbjct: 181 RDVYIGSANQDWKSLSQVKELGIYLTGCPKIAAHVKVYFDNLLKLA-FLNPTDYTRTIFD 240

Query: 241 ------------TQIINPDS----------------------WEPLINVAASLLLSLFRP 300
                       +  I+P+S                        P+I+   +L LS+  P
Sbjct: 241 HQWQTQRKVPCWSYFIDPESRCRQVQPFSSPLPPYVKFLHTLGYPIISDPYTLNLSIQTP 300

Query: 301 SREFG-------------PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFT 360
                             P+LLFGRYQSDEQAWSDTIKSVS+GATVRISTMDWLGQSEFT
Sbjct: 301 GSRLSTLLPHSCYLSFAPPELLFGRYQSDEQAWSDTIKSVSNGATVRISTMDWLGQSEFT 360

Query: 361 TPTIYWSSLSSAISEVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDC 420
            PTIYWSSLSSAISEVVFSKNATV+LLVSYWTHFVEGTD YLKSLLYSNILCNSSKFNDC
Sbjct: 361 NPTIYWSSLSSAISEVVFSKNATVRLLVSYWTHFVEGTDPYLKSLLYSNILCNSSKFNDC 420

Query: 421 FGKVEIKYYVVPGYNETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWD 478
           FGKVEIKYYVVPGYNETGPAL+ N TRT+N YPSFTRVQHGK+AVSDVRAHIGTSNLGWD
Sbjct: 421 FGKVEIKYYVVPGYNETGPALYHNRTRTENRYPSFTRVQHGKYAVSDVRAHIGTSNLGWD 480

BLAST of HG10014336 vs. ExPASy TrEMBL
Match: A0A6J1ILK6 (phospholipase D Y-like OS=Cucurbita maxima OX=3661 GN=LOC111476706 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.7e-223
Identity = 399/514 (77.63%), Postives = 431/514 (83.85%), Query Frame = 0

Query: 6   TLTVTTIFSALFFFISQFLPVLSFTQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL 65
           T+TVTTIFSALFFF SQF PVLSF+QPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL
Sbjct: 7   TVTVTTIFSALFFFTSQFPPVLSFSQPQCKAWLVQSIPTDMPQLQRVQGVLSTRDVFVWL 66

Query: 66  AGNSTERLDIIAQYWELLAGPDDSRSGDYGYSNEDLKKFGANKGYDVYSAIEKAADRNVN 125
           AGNST+RLDIIAQYW+LLA PDDSRSG+YGYSNEDLKKFGA +GYDVYSAIEKAADR+VN
Sbjct: 67  AGNSTQRLDIIAQYWDLLAAPDDSRSGEYGYSNEDLKKFGAYQGYDVYSAIEKAADRHVN 126

Query: 126 VRLVSHSGVYPNYGKEAADLASGRPNVQNVTLLLGDWYGSGIVHSKVWISDDKDVYIGSA 185
           VRLVSHSGVYPNYGKE ADLASGRPNVQ+VTLLLGDWYGSGI+H+KVWISDDKDVYIGSA
Sbjct: 127 VRLVSHSGVYPNYGKEPADLASGRPNVQDVTLLLGDWYGSGIIHTKVWISDDKDVYIGSA 186

Query: 186 NQDWKSLSQVKELGIYLTGCPRVAAHVKVTASTICEVSPYIRISVYIRSLH--------- 245
           NQDWKSLSQVKELGIYLTGCP +AAHVKV    + ++S ++  + Y R++          
Sbjct: 187 NQDWKSLSQVKELGIYLTGCPSIAAHVKVYFDNLLKLS-FLNHTDYTRTVFDHQWQIQRK 246

Query: 246 ----TQIINPDS----------------WEPLINVAASLLLSLFRPSREFG--------- 305
               +  I+PDS                  P I+   +L LS+  P   F          
Sbjct: 247 VPCWSYFIDPDSRCRSPLPPYVNIPHTLGYPFISDPYTLNLSIQTPGSSFSTLLPHSCYL 306

Query: 306 ----PQLLFGRYQSDEQAWSDTIKSVSSGATVRISTMDWLGQSEFTTPTIYWSSLSSAIS 365
               P+LLFGRYQSDEQAWSDTIKSVSSG  VRISTMDWLGQSEFTTPTI+WSSLSSAIS
Sbjct: 307 SFAPPELLFGRYQSDEQAWSDTIKSVSSGEIVRISTMDWLGQSEFTTPTIFWSSLSSAIS 366

Query: 366 EVVFSKNATVKLLVSYWTHFVEGTDSYLKSLLYSNILCNSSKFNDCFGKVEIKYYVVPGY 425
           EV+FSKNATVKLLVSYWTHFVE TD YLKSLLYSNILCNSSKFN CFGKVEIKYYVVPGY
Sbjct: 367 EVIFSKNATVKLLVSYWTHFVENTDPYLKSLLYSNILCNSSKFNHCFGKVEIKYYVVPGY 426

Query: 426 NETGPALHRNGTRTKNTYPSFTRVQHGKFAVSDVRAHIGTSNLGWDYFYVTSGVSFGTYN 478
           N +GPAL+ NGTRT NTYPSFTRVQHGKFAVS+VRAHIGTSNL WDYFYVTSGVSFGTYN
Sbjct: 427 NRSGPALYPNGTRTNNTYPSFTRVQHGKFAVSNVRAHIGTSNLAWDYFYVTSGVSFGTYN 486