HG10014285 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014285 vs. NCBI nr
Match: XP_038897085.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 586/738 (79.40%), Postives = 639/738 (86.59%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKE 60
           MD+NGN P KAVR SSS  ESEN G VVVEISSV+SSKE +D+NGYSV KQNRVDSQTKE
Sbjct: 1   MDVNGNNPSKAVRRSSSLKESENEGHVVVEISSVLSSKETKDENGYSVTKQNRVDSQTKE 60

Query: 61  PTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDSNM 120
           PTDS I H +DS LPP AN+P +IP S GTLT  RSLKRS  SRPKSRFGEQPR+ DS+M
Sbjct: 61  PTDSSIGHSNDSYLPPIANEPLKIPYSNGTLTLRRSLKRSYLSRPKSRFGEQPRYTDSDM 120

Query: 121 FEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLI 180
           FEEN +SLREQ GATSSRSS  +   AQ EEED E+    + K  K  KK KKVKVKTLI
Sbjct: 121 FEENDVSLREQIGATSSRSSVLNPPNAQSEEEDGED----IVKTEKSNKKDKKVKVKTLI 180

Query: 181 KLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIE 240
           KLVG   +I CLVASLTV RLKNCFFWGL+VWKWCLLATVI CG IFT WVMH+VV+LIE
Sbjct: 181 KLVGAFCLIACLVASLTVNRLKNCFFWGLKVWKWCLLATVIFCGKIFTTWVMHVVVNLIE 240

Query: 241 GNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLT 300
            NFLLKK VLYFVHGL+KSV+VT+WL LVLVTW SLF+  NHR+S    +GKILD IT T
Sbjct: 241 RNFLLKKNVLYFVHGLRKSVRVTLWLTLVLVTWASLFNWNNHRSSR--TVGKILDAITWT 300

Query: 301 LIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGAGSRN 360
           L+ALL+GA LWLVKTLLLKILASKFHK++FFDRIQE IFHHHVLQTL + PL EGA    
Sbjct: 301 LVALLIGAVLWLVKTLLLKILASKFHKNRFFDRIQESIFHHHVLQTLLKCPLKEGAEGTA 360

Query: 361 KFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQTLDESY 420
           KF+CCRFSL+SKKSDHKKVI  G IH+LQREKV+AWT+KVLIEAV SS+MSISQ LDES 
Sbjct: 361 KFSCCRFSLKSKKSDHKKVIDTGKIHQLQREKVSAWTIKVLIEAVTSSQMSISQILDES- 420

Query: 421 HNIADGEITDEMETASAVAHKILENVALGNMFIQEEDLKRFMVKEEVDLVLPHFEVDETR 480
            N+ADGEITDEME A AVA KI +N+A G  FIQEEDL +FMVKEE+DLVLPHFEVDETR
Sbjct: 421 SNVADGEITDEMEIAIAVACKIFKNIAHGKKFIQEEDLLKFMVKEEIDLVLPHFEVDETR 480

Query: 481 RIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLLMEIATS 540
           +ID+KAL  WVVKVYQERKTL HALNDTKTAV++LNNL  A++IIVTAVIWLLLMEIATS
Sbjct: 481 KIDKKALINWVVKVYQERKTLAHALNDTKTAVEELNNLAIALIIIVTAVIWLLLMEIATS 540

Query: 541 KVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEEMNILTT 600
           KVLVFLLSQLAVAAFMFG++CKTAFEALIFVFVMHPFDVGD C VDGV LLVEEMNILTT
Sbjct: 541 KVLVFLLSQLAVAAFMFGNACKTAFEALIFVFVMHPFDVGDFCVVDGVQLLVEEMNILTT 600

Query: 601 VFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEKIKRYLE 660
           VFLKLNNE VYYPNSVLATKPITNYYRSP+M DTIEFSISFATPLERIG MKEKIKRYLE
Sbjct: 601 VFLKLNNEKVYYPNSVLATKPITNYYRSPNMFDTIEFSISFATPLERIGAMKEKIKRYLE 660

Query: 661 KNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKRIFEELK 720
           KNPQHWHPNHSVVVKEIENVNK+ IAL+  HTMNFQDWSEK+RRR+ELVMELKRIFEELK
Sbjct: 661 KNPQHWHPNHSVVVKEIENVNKIKIALFAKHTMNFQDWSEKNRRRTELVMELKRIFEELK 720

Query: 721 INYDLLPQTVHLFPVQEQ 739
           INY+LLPQTVHLFPVQEQ
Sbjct: 721 INYNLLPQTVHLFPVQEQ 731

BLAST of HG10014285 vs. NCBI nr
Match: KAA0064529.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa] >TYK20061.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1026.9 bits (2654), Expect = 8.4e-296
Identity = 562/742 (75.74%), Postives = 622/742 (83.83%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEI--SSVVSSKEIRDDNGYSVPKQNRVDSQT 60
           MD+NGNKP+K VR SSSQ E EN GQVVV+I  SSVV  KE RD+N YSV  QNRVDSQ 
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KEPTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDS 120
           KE T S I HG+DS+ PPTAN+P +IPSS       RSLKRSI SRPKSRFGEQPR IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 -NMFEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEE--KTEPLNKKHKKVKKVKKVK 180
            +MFEENH SLREQ GATSSR+SAP+T KAQPEEED+E+  KTE LNKKH      KK K
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKH------KKWK 180

Query: 181 VKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 240
           VKT+IK V      GCLVASLTV RLKNCFF GL VWKWCLLATVI CG+IF  W  ++ 
Sbjct: 181 VKTMIKWVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVA 240

Query: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 300
           VSLIEGN LLKKKVLYFVHGLKKSVQ T+WLA VL TW  LF++ NHR+S     G+ILD
Sbjct: 241 VSLIEGNCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSR--TTGRILD 300

Query: 301 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 360
            IT TL+ALL+G+FLWL+KTLLLKILASKFHKD+FFDRIQE IFHHHVLQTL  SPL+EG
Sbjct: 301 AITWTLVALLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEG 360

Query: 361 AGSRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQT 420
           A S  KF+ C FSLES KSDHKK+I MG IH+LQREKV+AWTMKVL+EAV SS MSISQ 
Sbjct: 361 AESAAKFSRCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQI 420

Query: 421 LDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHF 480
           LDESY+N+ DGEI  EME AS VA KIL NVAL G  FIQEEDL++F+VKEE+D VLPHF
Sbjct: 421 LDESYYNVGDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHF 480

Query: 481 EVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLL 540
           EVDETRRI +KAL KWVVKVYQERKTL HAL DTKTAVKQLNNLVTA++IIV  VIWLLL
Sbjct: 481 EVDETRRIGKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLL 540

Query: 541 MEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEE 600
           MEIATSKVLVFLLSQLAVAAFMFG++CKT FEALIFVFVMHPFDVGDRC VDGV LLVEE
Sbjct: 541 MEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEE 600

Query: 601 MNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEK 660
           MNILTTVFLKLNNE VYYPNSVLATKPI+NYYRSPDM +T EFSISFATPLERIG MKEK
Sbjct: 601 MNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 660

Query: 661 IKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKR 720
           IK YLEKNPQHW PNH +VV+EIENVN++ IAL++ HTM++Q++ EK +RRSELVMELKR
Sbjct: 661 IKSYLEKNPQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKR 720

Query: 721 IFEELKINYDLLPQTVHLFPVQ 737
           IFEEL INY+L PQT+HLFPV+
Sbjct: 721 IFEELNINYNLPPQTIHLFPVE 734

BLAST of HG10014285 vs. NCBI nr
Match: XP_004141432.2 (mechanosensitive ion channel protein 10 [Cucumis sativus])

HSP 1 Score: 1026.9 bits (2654), Expect = 8.4e-296
Identity = 566/742 (76.28%), Postives = 626/742 (84.37%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEI--SSVVSSKEIRDDNGYSVPKQNRVDSQT 60
           MD+NGNKP K VR SSSQ E EN GQVVVEI  SSVV SKE RD N YSV KQ+RVDSQ 
Sbjct: 1   MDVNGNKP-KVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60

Query: 61  KEPTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDS 120
           KE T S I HG DS++PPTAN+P +IPSS  TLT  RSLKRSI SRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120

Query: 121 -NMFEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEE--KTEPLNKKHKKVKKVKKVK 180
            + FEE H SLREQTGATSSRSS+ +T KAQPEEED+E+  KTE LNKKH      KK K
Sbjct: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKH------KKWK 180

Query: 181 VKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 240
           VKT+IK +    +IGCLVASLTV RLKNCFF GL +WKWCLLATVI CG+I THW M++V
Sbjct: 181 VKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVV 240

Query: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 300
           VSLIEGNFLLKKKVLYFVHGLKKSVQVT+WLA VL TW  LF++RNHR+S     GKILD
Sbjct: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSR--TTGKILD 300

Query: 301 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 360
            IT TL+ALL+G+FLWLVKTLLLKILASKFHKD+FFDRIQE IFHHHVLQ L   PLM+ 
Sbjct: 301 AITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQE 360

Query: 361 AGSRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQT 420
             S  KF+ C FS E+KKSD KK+I  G IH LQREKV++WTMKVL+EAV SS MSISQ 
Sbjct: 361 VESAAKFSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQI 420

Query: 421 LDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHF 480
           LDESY+N+ DGEI  EME AS VA KIL NVAL G  FIQEEDL +F+VKEE+DLVLPHF
Sbjct: 421 LDESYYNVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHF 480

Query: 481 EVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLL 540
           EVDET+RI +KAL KWVVKV+QERKTL HAL DTKTAVKQLNNLVTA++IIV AVIWLLL
Sbjct: 481 EVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLL 540

Query: 541 MEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEE 600
           MEIATSKVLVFLLSQLAVAAFMFG++CKT FEALIFVFVMHPFDVGDRC VDGV LLVEE
Sbjct: 541 MEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEE 600

Query: 601 MNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEK 660
           MNILTTVFLKLNNE VYYPNSVLATKPI+NYYRSPDM +T EFSI+FATPLERIG MKEK
Sbjct: 601 MNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEK 660

Query: 661 IKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKR 720
           IKRYLEKNPQHW P+H +VVKEIENVN++ IALY+ HTM++QD+ EK +RRSELVMELKR
Sbjct: 661 IKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKR 720

Query: 721 IFEELKINYDLLPQTVHLFPVQ 737
           IFEELKINY LLPQT+HLFPV+
Sbjct: 721 IFEELKINYTLLPQTIHLFPVE 733

BLAST of HG10014285 vs. NCBI nr
Match: KAE8649918.1 (hypothetical protein Csa_012502 [Cucumis sativus])

HSP 1 Score: 1026.2 bits (2652), Expect = 1.4e-295
Identity = 566/742 (76.28%), Postives = 625/742 (84.23%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEI--SSVVSSKEIRDDNGYSVPKQNRVDSQT 60
           MD+NGNKP K VR SSSQ E EN GQVVVEI  SSVV SKE RD N YSV KQ+RVDSQ 
Sbjct: 1   MDVNGNKP-KVVRRSSSQKEGENAGQVVVEISNSSVVFSKETRDGNNYSVLKQDRVDSQN 60

Query: 61  KEPTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDS 120
           KE T S I HG DS++PPTAN+P +IPSS  TLT  RSLKRSI SRPKSRFGEQ R+ DS
Sbjct: 61  KESTGSSIDHGFDSHIPPTANEPLKIPSSNRTLTPRRSLKRSILSRPKSRFGEQSRYTDS 120

Query: 121 -NMFEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEE--KTEPLNKKHKKVKKVKKVK 180
            + FEE H SLREQTGATSSRSS+ +T KAQPEEED+E+  KTE LNKKH      KK K
Sbjct: 121 DDKFEEKHESLREQTGATSSRSSSLNTPKAQPEEEDEEDIVKTEQLNKKH------KKWK 180

Query: 181 VKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 240
           VKT+IK +    +IGCLVASLTV RLKNCFF GL +WKWCLLATVI CG+I THW M++V
Sbjct: 181 VKTVIKWIVVFCLIGCLVASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVV 240

Query: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 300
           VSLIEGNFLLKKKVLYFVHGLKKSVQVT+WLA VL TW  LF++RNHR+S     GKILD
Sbjct: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSR--TTGKILD 300

Query: 301 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 360
            IT TL+ALL+G+FLWLVKTLLLKILASKFHKD+FFDRIQE IFHHHVLQ L   PLM+ 
Sbjct: 301 AITWTLVALLIGSFLWLVKTLLLKILASKFHKDRFFDRIQESIFHHHVLQALLGPPLMQE 360

Query: 361 AGSRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQT 420
             S  KF+ C FS E+KKSD KK+I  G IH LQREKV++WTMKVL+EAV SS MSISQ 
Sbjct: 361 VESAAKFSRCLFSWENKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMSISQI 420

Query: 421 LDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHF 480
           LDESY+N+ DGEI  EME AS VA KIL NVAL G  FIQEEDL +F+VKEE+DLVLPHF
Sbjct: 421 LDESYYNVDDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHF 480

Query: 481 EVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLL 540
           EVDET+RI +KAL KWVVKV+QERKTL HAL DTKTAVKQLNNLVTA++IIV AVIWLLL
Sbjct: 481 EVDETKRIGKKALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLL 540

Query: 541 MEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEE 600
           MEIATSKVLVFLLSQLAVAAFMFG++CKT FEALIFVFVMHPFDVGDRC VDGV LLVEE
Sbjct: 541 MEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEE 600

Query: 601 MNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEK 660
           MNILTTVFLKLNNE VYYPNSVLATKPI+NYYRSPDM +T EFSI+FATPLERIG MKEK
Sbjct: 601 MNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEK 660

Query: 661 IKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKR 720
           IKRYLEKNPQHW P+H +VVKEIENVN++ IALY+ HTM++QD+ EK +RRSELVMELKR
Sbjct: 661 IKRYLEKNPQHWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKR 720

Query: 721 IFEELKINYDLLPQTVHLFPVQ 737
           IFEELKINY LLPQT+HLFP Q
Sbjct: 721 IFEELKINYTLLPQTIHLFPFQ 733

BLAST of HG10014285 vs. NCBI nr
Match: XP_038899727.1 (mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida] >XP_038899728.1 mechanosensitive ion channel protein 10-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1024.2 bits (2647), Expect = 5.4e-295
Identity = 556/739 (75.24%), Postives = 629/739 (85.12%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKE 60
           MD+N N+ LK V GS SQ ESENGGQV V ISSV        +NGYSVPKQNRVDSQTKE
Sbjct: 1   MDVNSNRALKVVGGSLSQMESENGGQVAVNISSV--------ENGYSVPKQNRVDSQTKE 60

Query: 61  PTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDSNM 120
           P DS I + +DS L PTANKPP+IPSSKGTLT  RSL+R I S+ KSRFGEQP +IDS++
Sbjct: 61  PIDSSIGYSYDSQLAPTANKPPKIPSSKGTLTPRRSLRRPILSKSKSRFGEQPGYIDSDV 120

Query: 121 FEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEE--KTEPLNKKHKKVKKVKKVKVKT 180
           FEENH+SLREQ G+TSSRSS  +T KAQPEEEDD    + E LN KH      KKVKVKT
Sbjct: 121 FEENHVSLREQIGSTSSRSSTVNTPKAQPEEEDDAGIFEIEQLNNKH------KKVKVKT 180

Query: 181 LIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSL 240
           +I+ +G   IIGCLVASLTV   KN F WGLRVWKW LLATVILCG+IFT WVM+++V L
Sbjct: 181 VIEWIGVFCIIGCLVASLTVNPWKNRFLWGLRVWKWLLLATVILCGVIFTRWVMNVIVFL 240

Query: 241 IEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTIT 300
           IE NFLLKKKVLYFVHGLKKSVQVT+WL LVLVTW SLFD+RNH+ S+  I  KILD++T
Sbjct: 241 IERNFLLKKKVLYFVHGLKKSVQVTLWLTLVLVTWGSLFDQRNHKISS-RITAKILDSVT 300

Query: 301 LTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGAGS 360
            TL++LL+GA L LVKTLLLKILASKFH ++FFDR+QE IF H+VLQTLSR   +  AG+
Sbjct: 301 WTLVSLLIGAVLLLVKTLLLKILASKFHMNRFFDRVQESIFLHYVLQTLSRGVFIGEAGT 360

Query: 361 RNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQTLDE 420
            +K +  R SL+SKKSD K+VI +G IH+L+REKV+AWTMKVL++AV SSEMS+SQ LDE
Sbjct: 361 TSKSSSGRLSLKSKKSDRKEVIDLGKIHQLKREKVSAWTMKVLVDAVTSSEMSVSQILDE 420

Query: 421 SYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHFEVD 480
           SY ++ADGEI DEME A A A+KI  NVAL GN FI+EEDL +FM+KEEVDLVLPHFE+D
Sbjct: 421 SYQDVADGEIADEMEVAKAAAYKIFNNVALPGNKFIEEEDLLKFMIKEEVDLVLPHFEID 480

Query: 481 ETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLLMEI 540
           +TRRID+KALT WVVKVYQ RKTL HAL DTKTAVKQLNNLVTA++IIVTAVIWLLLMEI
Sbjct: 481 DTRRIDKKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALIIIVTAVIWLLLMEI 540

Query: 541 ATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEEMNI 600
           AT+KVLVFLL+QLAVAAFMFG++CKTAFEALIFVFVMHPFDVGDRC VDGV LLVEEMNI
Sbjct: 541 ATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 600

Query: 601 LTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEKIKR 660
           LTTVFLKLNNE VYYPNSVLATKPITNYYRSPDM +TIEFSI F TPLERIGVMK+KIKR
Sbjct: 601 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGETIEFSIGFTTPLERIGVMKDKIKR 660

Query: 661 YLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKRIFE 720
           YLEKN QHW+PNHSVVV+EIENVNK+ IALYTNHTMNFQDW+EK+RRR+ELVMELKRIFE
Sbjct: 661 YLEKNSQHWYPNHSVVVQEIENVNKIKIALYTNHTMNFQDWTEKNRRRTELVMELKRIFE 720

Query: 721 ELKINYDLLPQTVHLFPVQ 737
           ELKINY+LLPQTVHL  V+
Sbjct: 721 ELKINYNLLPQTVHLIQVE 724

BLAST of HG10014285 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 3.0e-179
Identity = 384/743 (51.68%), Postives = 503/743 (67.70%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKEPTDSCIAHGHDSNLPP 76
           S N    GG VV+ +    +S+  ++    + P+  +    +K P+         S L  
Sbjct: 6   SSNGGGGGGDVVINVPVEEASRRSKE---MASPESEKGVPFSKSPSPEI------SKLVG 65

Query: 77  TANKPPEIPSSKGT-LTSTRSLKRSIPSRPKSRFGEQPRFIDSNMFEENHLSLREQTGA- 136
           + NKPP  P+     LT  +S  RS+ S+PKSRF +    +D+++ EE    +REQ GA 
Sbjct: 66  SPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAG 125

Query: 137 -------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIK 196
                         S  S AP T      E+D++E+     K +++++   K+    LI+
Sbjct: 126 FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMR--SKISTLALIE 185

Query: 197 LVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIEG 256
              FV I+  LVASLT+  LK+  FWGL VWKWC+L  VI  GM+ T+W M ++V LIE 
Sbjct: 186 SAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIET 245

Query: 257 NFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLTL 316
           NFLL++KVLYFVHGLKKSVQV IWL L+LV W+ LF   NH         K+L  IT TL
Sbjct: 246 NFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTL 305

Query: 317 IALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGA---GS 376
           I++L GAF WLVKTLLLKILA+ F+ + FFDRIQ+ +FH +VLQTLS  PLME A   G 
Sbjct: 306 ISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGR 365

Query: 377 RNKFNCCRFSLESKKS--DHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS-ISQT 436
                   F+   KK     KKVI MG +H+++REKV+AWTM+VL+EAV +S +S IS T
Sbjct: 366 EPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 425

Query: 437 LDESYH----NIADGEITDEMETASAVAHKILENVALGNM-FIQEEDLKRFMVKEEVDLV 496
           LDE+ +      AD EIT EME A A A+ +  NVA     +I+EEDL RFM+KEEVDLV
Sbjct: 426 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 485

Query: 497 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 556
            P F+   ET RI  KA T+WVVKVY  R+ L H+LNDTKTAVKQLN LVTAIL++VT V
Sbjct: 486 FPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVV 545

Query: 557 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 616
           IWLLL+E+AT+KVL+F  +QL   AF+ GS+CK  FE+++FVFVMHP+DVGDRC VDGV 
Sbjct: 546 IWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVA 605

Query: 617 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 676
           +LVEEMN+LTTVFLKLNNE VYYPN+VLATKPI+NY+RSP+M +T+EFSISF+TP+ +I 
Sbjct: 606 MLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIA 665

Query: 677 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
            +KE+I  YLE+NPQHW P HSVVVKEIEN+NK+ +ALY++HT+ FQ+  E++ RR+EL 
Sbjct: 666 HLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELS 725

BLAST of HG10014285 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.8e-166
Identity = 363/745 (48.72%), Postives = 490/745 (65.77%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDD---NGYSVPKQNRVDSQTKEPTDSCIAHGHDSN 76
           ++    NG +VV+ +S    SK+ R     N  + P  +    ++K      I       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRF-IDSNMFEENHLSLREQT 136
              + +KPP+IPS +G L   +SL RSI S+PKSRFGEQ  F  DS   E    SLREQ 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 GA---------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVK 196
           GA                S+RS A +      EEE DE   E + KK  K+ +VK+  +K
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDE--NEEIYKK-VKLHRVKRSGMK 181

Query: 197 TL--IKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 256
            L  ++LV F+ I+G L+ SLT+  +     WGL  WKWC+L  V L GM+ T+W MH V
Sbjct: 182 PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFV 241

Query: 257 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 316
           V +IE N+LL+KKVLYFVHGLKK+VQV IW +LVL+ W+ LFD    R        + LD
Sbjct: 242 VFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRK---TKRFLD 301

Query: 317 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 376
            IT T+++LLVG+ L+LVKT  LK+LASKF+   FF+RIQE +FH +VLQTLS  PL+E 
Sbjct: 302 FITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEE 361

Query: 377 AGSRNKFNCCRFSLESKKSD----HKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS 436
           A +  +         ++  D     KKVI MG +HR+++EKV+AWTM+VLIEAV +S +S
Sbjct: 362 AENVGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGIS 421

Query: 437 -ISQTLDE--SYHNIADGEITDEMETASAVAHKILENVALGN-MFIQEEDLKRFMVKEEV 496
            IS TLDE  +     D EIT+EME A A A+ +  NVA  N  +I+E+DL RFM+KEEV
Sbjct: 422 TISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEV 481

Query: 497 DLVLPHFEVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVT 556
           DLVLP  E  +T +I  K  T+WVV VY  RKT+ H+LNDTKTAVKQL+ L+T IL ++T
Sbjct: 482 DLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVIT 541

Query: 557 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDG 616
            ++W++L++IA++K+L+   SQ    AFM GS+CK  FE+ +FVFVMHP+DVGDRC VDG
Sbjct: 542 FIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDG 601

Query: 617 VHLLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLER 676
           V LLVEE+++LTTVFLK++NE V+YPNSVL +KPI+N+YRSPDM D ++F I+F+TP E+
Sbjct: 602 VMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEK 661

Query: 677 IGVMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSE 733
           IG +K KI  YL  N QHW+P   V+V+ IEN+NK+ + +   HT+NFQ + EKS RR+ 
Sbjct: 662 IGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTA 721

BLAST of HG10014285 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.4e-115
Identity = 256/623 (41.09%), Postives = 371/623 (59.55%), Query Frame = 0

Query: 150 EEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGL 209
           EEEDD    E L ++++K     K+ +  +++ +  + II   V +L +  L+    W L
Sbjct: 218 EEEDDPFAAEDLPEEYRK----DKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWEL 277

Query: 210 RVWKWCLLATVILCGMIFTHWVMHIVVSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALV 269
           ++WKW  +  V++CG + + W++ IVV  IE NFLL+K+VLYFV+G++K+VQ  +WL LV
Sbjct: 278 QLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLV 337

Query: 270 LVTWVSLFDRRNHRNSNLGIIGKILDTITLTLIALLVGAFLWLVKTLLLKILASKFHKDQ 329
           L+ W  LFD +  + +N     K L  +T   + LLVG  LWLVKTLL+K+LAS FH   
Sbjct: 338 LLAWHFLFDEKVAKAANT----KALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMST 397

Query: 330 FFDRIQEFIFHHHVLQTLSRSPLMEGAGSRNKFNCCRFSLESKK---------------- 389
           +FDRIQE +F  +V++TLS  PL+E    +N+    R S+E KK                
Sbjct: 398 YFDRIQESLFTQYVIETLSGPPLIE--IQKNEEEEERISVEVKKFQNPGGVEIQSGAQKS 457

Query: 390 --------------------SDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSI- 449
                                   K I +  +H+L  + V+AW MK L+  + +  ++  
Sbjct: 458 PMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTL 517

Query: 450 -SQTLDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLV 509
             Q  D S  +    +I  E E A   A KI  NVA  G+ FI   D+ RF+  +E    
Sbjct: 518 DEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKT 577

Query: 510 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 569
           L  FE   ET RI + +L  WVV  ++ER+ L   LNDTKTAV +L+ +V  ++ I+  V
Sbjct: 578 LSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILV 637

Query: 570 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 629
           IWL+++ I ++K LV + SQ+ V AF+FG+ CK  FE++I++FV+HPFDVGDRC +DGV 
Sbjct: 638 IWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQ 697

Query: 630 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 689
           ++VEEMNILTTVFL+ +N+ V YPNS+L TK I NYYRSPDM D IEFSI   TP E+I 
Sbjct: 698 MVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKII 757

Query: 690 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
           ++K++I  Y+E    HW+P   +V K++E++N V IA++  H MN QD  EK  RRS+LV
Sbjct: 758 LIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLV 817

BLAST of HG10014285 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 411.0 bits (1055), Expect = 2.9e-113
Identity = 245/624 (39.26%), Postives = 378/624 (60.58%), Query Frame = 0

Query: 147 AQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIKLVGFVFIIGCLVASLTVKRLKNCFF 206
           A  +EEDD    E +  ++K+     K+   TL++ +  V II  L  SL+++  K    
Sbjct: 273 ADIDEEDDPLAEEDVPDEYKR----GKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRV 332

Query: 207 WGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIEGNFLLKKKVLYFVHGLKKSVQVTIWL 266
           W L +WKW +   V++CG + + W + IVV  IE NFLL+K+VLYFV+G++++VQ  +WL
Sbjct: 333 WNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWL 392

Query: 267 ALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLTLIALLVGAFLWLVKTLLLKILASKFH 326
            LVL+ W  LFD++  R +      + L  +T  L+  L+   LWL+KTL++K+LAS FH
Sbjct: 393 GLVLLAWHFLFDKKVQRETR----SRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFH 452

Query: 327 KDQFFDRIQEFIFHHHVLQTLSRSPL-------------------MEGAGSRNKFNCC-- 386
              +FDRIQE +F+ +V++TLS  P+                   M+ AG+    + C  
Sbjct: 453 VSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAA 512

Query: 387 -------------RFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAV--ISSEM 446
                        + S    KS     I M  +HR+  + ++AW MK L++ V  +S   
Sbjct: 513 AFPPGKSGRVMNPKLSPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTT 572

Query: 447 SISQTLDESYHNIADGEITDEMETASAVAHKILENV-ALGNMFIQEEDLKRFMVKEEVDL 506
              Q L+ +Y + +  +I  E E A A A KI +NV   G  +I  EDL RF+ ++E   
Sbjct: 573 LDEQMLESTYEDESTRQIRSEKE-AKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMK 632

Query: 507 VLPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTA 566
            +  FE   E +RI + AL  W+V  ++ER+ L   LNDTKTAV +L++++  +  IV  
Sbjct: 633 TMGLFEGAPENKRISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIV 692

Query: 567 VIWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGV 626
           VIWL+L+EIA+SKVL+F+ SQ+ + AF+FG++ KT FE++IF+F++HP+DVGDRC +D V
Sbjct: 693 VIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSV 752

Query: 627 HLLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERI 686
            L+VEEMNILTTVFL+ +N  + YPNS+L  K I NYYRSPDM D IEF +   TPLE+I
Sbjct: 753 QLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKI 812

Query: 687 GVMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSEL 733
            V+K++I  Y++  P++W+P   ++VK++E+++ V +A++  H +N QD +E+  RR+ L
Sbjct: 813 SVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVL 872

BLAST of HG10014285 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 4.6e-111
Identity = 258/693 (37.23%), Postives = 387/693 (55.84%), Query Frame = 0

Query: 93  STRSLKRSIPSRPKSRFGEQPRFIDSNMFEENHLSLREQTGATSSRSSAPSTTKAQPEEE 152
           ST   K  +  + +SR  + P     +M               S R++ P T   Q   +
Sbjct: 174 STMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPN-QGGSK 233

Query: 153 DDEEKTEPLNKKH-KKVKKVKKVKVKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRV 212
           D EE+ +P +++   +  + +K+ V  +I+ +  + II  L+ SL +  L+    W L +
Sbjct: 234 DLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLAL 293

Query: 213 WKWCLLATVILCGMIFTHWVMHIVVSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLV 272
           WKW ++  V++CG + + W++ + V  +E NFL +KKVLYFV+G++K VQ  +WL LVL+
Sbjct: 294 WKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLI 353

Query: 273 TWVSLFDRRNHRNSNLGIIGKILDTITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFF 332
            W  LFD++  R         +L  +T  LI LLV   +WL+KTLL+K+LAS FH   +F
Sbjct: 354 AWHFLFDKKVEREMR----STVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYF 413

Query: 333 DRIQEFIFHHHVLQTLSRSPLME------------------------------------- 392
           DRIQE +F  +V++TLS  P +E                                     
Sbjct: 414 DRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQVT 473

Query: 393 -GAG------SRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVIS 452
            G+G      SR   +       SKK   ++ I +  + R+  + V+AW MK L+  +  
Sbjct: 474 VGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKK 533

Query: 453 SEMSISQTLDESYHNIADGE------ITDEMETASAVAHKILENVA-LGNMFIQEEDLKR 512
             +S   TLDE   +    E      I  E E A   A KI +NVA  G+ +I  ED  R
Sbjct: 534 GTLS---TLDEQIQDTTTQEDDKATQIRSEFE-AKLAARKIFQNVAEPGSRYIYMEDFMR 593

Query: 513 FMVKEEVDLVLPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLV 572
           F+ ++E +  +  FE   E  +I +  L  WVV  ++ER+ L   LNDTKTAV +L+ +V
Sbjct: 594 FLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIV 653

Query: 573 TAILIIVTAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDV 632
             ++ IV  +IWLL++ IAT+K L+ + SQL +  F+FG+SCKT FEA+IFVFVMHPFDV
Sbjct: 654 DVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDV 713

Query: 633 GDRCAVDGVHLLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSI 692
           GDRC +DGV ++VEEMNILTTVFL+ +N+ + YPNS+L TKPI NYYRSPDM D IEF +
Sbjct: 714 GDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFV 773

Query: 693 SFATPLERIGVMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWS 733
             ATP E+   ++++I  Y++    HWHP+  +V +++  +N V IA++  H MN Q+  
Sbjct: 774 HIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMG 833

BLAST of HG10014285 vs. ExPASy TrEMBL
Match: A0A5A7VG97 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001830 PE=3 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 4.1e-296
Identity = 562/742 (75.74%), Postives = 622/742 (83.83%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEI--SSVVSSKEIRDDNGYSVPKQNRVDSQT 60
           MD+NGNKP+K VR SSSQ E EN GQVVV+I  SSVV  KE RD+N YSV  QNRVDSQ 
Sbjct: 1   MDVNGNKPMKVVRRSSSQKEGENAGQVVVDISTSSVVFPKETRDENNYSVLNQNRVDSQN 60

Query: 61  KEPTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDS 120
           KE T S I HG+DS+ PPTAN+P +IPSS       RSLKRSI SRPKSRFGEQPR IDS
Sbjct: 61  KESTGSSIDHGYDSHRPPTANQPLKIPSSNRPPMPRRSLKRSILSRPKSRFGEQPRHIDS 120

Query: 121 -NMFEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEE--KTEPLNKKHKKVKKVKKVK 180
            +MFEENH SLREQ GATSSR+SAP+T KAQPEEED+E+  KTE LNKKH      KK K
Sbjct: 121 EDMFEENHESLREQIGATSSRTSAPNTPKAQPEEEDEEDIVKTEQLNKKH------KKWK 180

Query: 181 VKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 240
           VKT+IK V      GCLVASLTV RLKNCFF GL VWKWCLLATVI CG+IF  W  ++ 
Sbjct: 181 VKTMIKWVVVFCPTGCLVASLTVNRLKNCFFLGLEVWKWCLLATVIFCGLIFIRWATNVA 240

Query: 241 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 300
           VSLIEGN LLKKKVLYFVHGLKKSVQ T+WLA VL TW  LF++ NHR+S     G+ILD
Sbjct: 241 VSLIEGNCLLKKKVLYFVHGLKKSVQTTLWLASVLSTWEPLFNQSNHRSSR--TTGRILD 300

Query: 301 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 360
            IT TL+ALL+G+FLWL+KTLLLKILASKFHKD+FFDRIQE IFHHHVLQTL  SPL+EG
Sbjct: 301 AITWTLVALLIGSFLWLIKTLLLKILASKFHKDRFFDRIQESIFHHHVLQTLLGSPLLEG 360

Query: 361 AGSRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQT 420
           A S  KF+ C FSLES KSDHKK+I MG IH+LQREKV+AWTMKVL+EAV SS MSISQ 
Sbjct: 361 AESAAKFSRCLFSLESNKSDHKKLIDMGKIHQLQREKVSAWTMKVLVEAVSSSAMSISQI 420

Query: 421 LDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHF 480
           LDESY+N+ DGEI  EME AS VA KIL NVAL G  FIQEEDL++F+VKEE+D VLPHF
Sbjct: 421 LDESYYNVGDGEIDHEMEIASVVASKILRNVALPGKKFIQEEDLQKFLVKEEIDFVLPHF 480

Query: 481 EVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLL 540
           EVDETRRI +KAL KWVVKVYQERKTL HAL DTKTAVKQLNNLVTA++IIV  VIWLLL
Sbjct: 481 EVDETRRIGKKALKKWVVKVYQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMTVIWLLL 540

Query: 541 MEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEE 600
           MEIATSKVLVFLLSQLAVAAFMFG++CKT FEALIFVFVMHPFDVGDRC VDGV LLVEE
Sbjct: 541 MEIATSKVLVFLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVQLLVEE 600

Query: 601 MNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEK 660
           MNILTTVFLKLNNE VYYPNSVLATKPI+NYYRSPDM +T EFSISFATPLERIG MKEK
Sbjct: 601 MNILTTVFLKLNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSISFATPLERIGAMKEK 660

Query: 661 IKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKR 720
           IK YLEKNPQHW PNH +VV+EIENVN++ IAL++ HTM++Q++ EK +RRSELVMELKR
Sbjct: 661 IKSYLEKNPQHWRPNHLLVVREIENVNEIKIALFSTHTMSYQEYGEKLKRRSELVMELKR 720

Query: 721 IFEELKINYDLLPQTVHLFPVQ 737
           IFEEL INY+L PQT+HLFPV+
Sbjct: 721 IFEELNINYNLPPQTIHLFPVE 734

BLAST of HG10014285 vs. ExPASy TrEMBL
Match: A0A6J1C8Z8 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111009404 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.8e-291
Identity = 558/753 (74.10%), Postives = 627/753 (83.27%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNR-VDSQTK 60
           MD+NGNKPLKAVR SSSQ ESENGGQVVVEIS VV SKE RD+NG SVP QNR VDSQ K
Sbjct: 1   MDVNGNKPLKAVRRSSSQKESENGGQVVVEISGVVPSKETRDENGCSVPSQNRVVDSQNK 60

Query: 61  EPTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGE-QPRFIDS 120
            PTDS I          +ANKPP+IP+S GTL   +SLKRSI S+PKSRFGE QP +IDS
Sbjct: 61  GPTDSSIG---------SANKPPKIPNSSGTLMPRKSLKRSIQSKPKSRFGEQQPYYIDS 120

Query: 121 NMFEENHLSLREQTGATSSRSSAP------STTKAQPEEEDDEE--KTEPLNKKHKKVKK 180
           +MFEE+ LSLREQ GATSSR ++P      +T +AQ EEED++   KTE L+K     +K
Sbjct: 121 DMFEEDRLSLREQIGATSSRRASPGHKSALTTPRAQAEEEDEDAIWKTEQLSK-----EK 180

Query: 181 VKKVKVKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHW 240
            KK+K+KTL+K VG   IIGCLVASLTV RL+NCF WGL +WKWCLLATVILCGMIFT W
Sbjct: 181 HKKLKMKTLVKWVGVFCIIGCLVASLTVNRLRNCFLWGLEIWKWCLLATVILCGMIFTKW 240

Query: 241 VMHIVVSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGII 300
           VM+++V LIE NFLLKKKVLYFVHGLKK VQVT+WL LVL TW SLFDR NHR       
Sbjct: 241 VMNVIVFLIERNFLLKKKVLYFVHGLKKIVQVTLWLTLVLATWASLFDRSNHRVLRSKTA 300

Query: 301 GKILDTITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRS 360
           GKILD  T TL++LL+GAFLWLVKTLLLKILASKFH ++FFDRIQE IFHHHVLQTLSR 
Sbjct: 301 GKILDAGTWTLVSLLIGAFLWLVKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLSRP 360

Query: 361 PLMEGA--GSRNKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISS 420
           PLM  A   +  K +  R SL+ KKSDHKKVI MG IH+L+REKV+AWTMKVL++AV SS
Sbjct: 361 PLMGVAECAASAKSSSGRLSLKGKKSDHKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSS 420

Query: 421 EMSISQTLDESYHN----IADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMV 480
           E+SISQ LDESY N    +AD +ITDEME A A A++I  NVAL GN FI+EEDL +FM+
Sbjct: 421 ELSISQLLDESYQNGGAEVADSDITDEMEVARAAAYRIFNNVALPGNKFIEEEDLLKFMI 480

Query: 481 KEEVDLVLPHFEVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAIL 540
           KEEVDLVLP FEV +TR+ID KALT WVVKVYQ RKTL HAL DTKTAVKQLNNLVTA+L
Sbjct: 481 KEEVDLVLPLFEVADTRQIDRKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVTALL 540

Query: 541 IIVTAVIWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRC 600
           IIVTAV+WLLLMEIAT+KVLVFLLSQLAVAAFMFG++CKT FEALIFVFVMHPFDVGDRC
Sbjct: 541 IIVTAVVWLLLMEIATTKVLVFLLSQLAVAAFMFGNTCKTTFEALIFVFVMHPFDVGDRC 600

Query: 601 AVDGVHLLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFAT 660
            VDGV LLVEEMNILTTVFLKL+NE VYYPNSVL+TKPITNYYRSPDM DT+EFSISF T
Sbjct: 601 VVDGVPLLVEEMNILTTVFLKLSNEKVYYPNSVLSTKPITNYYRSPDMSDTVEFSISFTT 660

Query: 661 PLERIGVMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSR 720
           PLERIG MKE+IKRYLEKN QHWHPNH VVVKEIE+VNK+ IALY NHT+NFQ+W+EK+R
Sbjct: 661 PLERIGAMKERIKRYLEKNSQHWHPNHDVVVKEIEDVNKIKIALYVNHTLNFQEWAEKNR 720

Query: 721 RRSELVMELKRIFEELKINYDLLPQTVHLFPVQ 737
           RR+ELVMELK+IFEEL INY+LLPQTVHLFPV+
Sbjct: 721 RRTELVMELKKIFEELNINYNLLPQTVHLFPVE 739

BLAST of HG10014285 vs. ExPASy TrEMBL
Match: A0A1S4DZ48 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103493697 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 3.3e-290
Identity = 547/739 (74.02%), Postives = 620/739 (83.90%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKE 60
           MD+NGNKP   +R SSSQ ESENGG+VVV++S V        +N YSVPKQNRVDSQTKE
Sbjct: 1   MDVNGNKP---IRRSSSQKESENGGEVVVQLSGV--------ENEYSVPKQNRVDSQTKE 60

Query: 61  PTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDSNM 120
           PT S + +G+ S L PTANKPP+IP S GTLT  RSL+RS  S+PKSRFGEQP +IDS+M
Sbjct: 61  PTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDM 120

Query: 121 F-EENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTL 180
           F EENH+SLREQ GATSSRSS  +T KAQPE ED   +   LN+KH      KK KVKT+
Sbjct: 121 FEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDG--RNMGLNEKH------KKAKVKTV 180

Query: 181 IKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLI 240
            K +G   II CLVASLTVK LKN F WGL+VWKWCLLATVI CG+IFT W+M++VV LI
Sbjct: 181 FKWIGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLI 240

Query: 241 EGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITL 300
           E NFLLKKKVLYFVHGLKKSVQVT+WL+LVL TW SLFDRRNH  S+  I  KILD +T 
Sbjct: 241 ERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTW 300

Query: 301 TLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGAGSR 360
           TL +LL+GAFLWL+KTLLLKILASKFH ++FFDRIQE +F HHVLQTL R P +E   S 
Sbjct: 301 TLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDE-ST 360

Query: 361 NKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQTL-DE 420
            KF CCRF  ESK+SD KKVI MG IH+L+REKV+AWTMKVL++AV SSEMSISQ L DE
Sbjct: 361 AKFRCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDE 420

Query: 421 SYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHFEVD 480
           SY ++ADG+IT+EM  A   A +I +NVAL GN FI+E DL  FM+ EEV+LV PHFEVD
Sbjct: 421 SYRDVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVD 480

Query: 481 ETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLLMEI 540
           +TR+ID KALT WVVKVYQ RKTL HAL DTKTAVKQLNNL+TA++IIVTA+IWLLLMEI
Sbjct: 481 KTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEI 540

Query: 541 ATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEEMNI 600
           AT+KVLVFLL+QLAVAAFMFG++CKTAFEALIFVFVMHPFDVGDRC VDGV LLVEEMNI
Sbjct: 541 ATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 600

Query: 601 LTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEKIKR 660
           LTTVFLKLNNE VYYPNSVLATKPITNYYRSPDM DT+EFSI F TP+ERIG MKE+IKR
Sbjct: 601 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKR 660

Query: 661 YLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKRIFE 720
           YLE+NPQHW+PNH+VVVKEIENVNK+ IALYTNHTMNFQDW+EK+RRR+ELVMELKRIFE
Sbjct: 661 YLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFE 719

Query: 721 ELKINYDLLPQTVHLFPVQ 737
           ELKINY+LLPQTVHLFPV+
Sbjct: 721 ELKINYNLLPQTVHLFPVE 719

BLAST of HG10014285 vs. ExPASy TrEMBL
Match: A0A5D3D991 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001820 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 3.3e-290
Identity = 547/739 (74.02%), Postives = 620/739 (83.90%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKE 60
           MD+NGNKP   +R SSSQ ESENGG+VVV++S V        +N YSVPKQNRVDSQTKE
Sbjct: 1   MDVNGNKP---IRRSSSQKESENGGEVVVQLSGV--------ENEYSVPKQNRVDSQTKE 60

Query: 61  PTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDSNM 120
           PT S + +G+ S L PTANKPP+IP S GTLT  RSL+RS  S+PKSRFGEQP +IDS+M
Sbjct: 61  PTGSSVGYGNASQLAPTANKPPKIPISNGTLTPRRSLRRSTLSKPKSRFGEQPGYIDSDM 120

Query: 121 F-EENHLSLREQTGATSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTL 180
           F EENH+SLREQ GATSSRSS  +T KAQPE ED   +   LN+KH      KK KVKT+
Sbjct: 121 FEEENHVSLREQIGATSSRSSNLNTPKAQPEGEDG--RNMGLNEKH------KKAKVKTV 180

Query: 181 IKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLI 240
            K +G   II CLVASLTVK LKN F WGL+VWKWCLLATVI CG+IFT W+M++VV LI
Sbjct: 181 FKWIGVFCIISCLVASLTVKPLKNRFLWGLKVWKWCLLATVIFCGLIFTRWIMNVVVFLI 240

Query: 241 EGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITL 300
           E NFLLKKKVLYFVHGLKKSVQVT+WL+LVL TW SLFDRRNH  S+  I  KILD +T 
Sbjct: 241 ERNFLLKKKVLYFVHGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTW 300

Query: 301 TLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGAGSR 360
           TL +LL+GAFLWL+KTLLLKILASKFH ++FFDRIQE +F HHVLQTL R P +E   S 
Sbjct: 301 TLASLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPPVEDE-ST 360

Query: 361 NKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQTL-DE 420
            KF CCRF  ESK+SD KKVI MG IH+L+REKV+AWTMKVL++AV SSEMSISQ L DE
Sbjct: 361 AKFRCCRFCFESKESDRKKVIDMGKIHQLKREKVSAWTMKVLVDAVTSSEMSISQILDDE 420

Query: 421 SYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHFEVD 480
           SY ++ADG+IT+EM  A   A +I +NVAL GN FI+E DL  FM+ EEV+LV PHFEVD
Sbjct: 421 SYRDVADGDITNEMNVAKEAAKEIFKNVALPGNKFIEERDLLAFMIPEEVNLVWPHFEVD 480

Query: 481 ETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLLMEI 540
           +TR+ID KALT WVVKVYQ RKTL HAL DTKTAVKQLNNL+TA++IIVTA+IWLLLMEI
Sbjct: 481 KTRKIDMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIIVTAIIWLLLMEI 540

Query: 541 ATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEEMNI 600
           AT+KVLVFLL+QLAVAAFMFG++CKTAFEALIFVFVMHPFDVGDRC VDGV LLVEEMNI
Sbjct: 541 ATTKVLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNI 600

Query: 601 LTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEKIKR 660
           LTTVFLKLNNE VYYPNSVLATKPITNYYRSPDM DT+EFSI F TP+ERIG MKE+IKR
Sbjct: 601 LTTVFLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKR 660

Query: 661 YLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKRIFE 720
           YLE+NPQHW+PNH+VVVKEIENVNK+ IALYTNHTMNFQDW+EK+RRR+ELVMELKRIFE
Sbjct: 661 YLEENPQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTELVMELKRIFE 719

Query: 721 ELKINYDLLPQTVHLFPVQ 737
           ELKINY+LLPQTVHLFPV+
Sbjct: 721 ELKINYNLLPQTVHLFPVE 719

BLAST of HG10014285 vs. ExPASy TrEMBL
Match: A0A6J1J491 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111481199 PE=3 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.3e-289
Identity = 551/739 (74.56%), Postives = 616/739 (83.36%), Query Frame = 0

Query: 1   MDLNGNKPLKAVRGSSSQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKE 60
           MDLNGN PLK +R SSSQ ESENG QVVVEIS        RD+NG+SVPKQNRVDSQTKE
Sbjct: 1   MDLNGNTPLKPIRRSSSQKESENGSQVVVEIS--------RDENGHSVPKQNRVDSQTKE 60

Query: 61  PTDSCIAHGHDSNLPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRFIDSNM 120
           P DS I +G+DS+L PTANKPP+IP S GTLT  +SLKRSI S+PKSRFGEQ   IDS+ 
Sbjct: 61  PMDSSIGYGYDSHLTPTANKPPKIPGSVGTLTPRKSLKRSILSKPKSRFGEQSSVIDSDT 120

Query: 121 FEENHLSLREQTGATSSRSSAPSTTKAQPEEEDDE-EKTEPLNKKHKKVKKVKKVKVKTL 180
           FEEN LS R+Q  A SSR S  +  K   EE DDE  K E    K KKVK  KKVK  TL
Sbjct: 121 FEENRLSFRDQISANSSRRSTLNMPKEHHEEGDDEFFKIE----KFKKVKH-KKVKAITL 180

Query: 181 IKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLI 240
           IK VG   IIGCLVASLT+KRLKN F WG+ +WKWCLLATVILCGMIFTH VM+++V LI
Sbjct: 181 IKWVGAFCIIGCLVASLTIKRLKNYFLWGIEIWKWCLLATVILCGMIFTHRVMNVIVFLI 240

Query: 241 EGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITL 300
           E NFLL+KKV YFVHGLKKSVQVT+WL LVLVTWVSLFDR +HR     I GKILD IT 
Sbjct: 241 ERNFLLQKKVFYFVHGLKKSVQVTLWLTLVLVTWVSLFDRSSHRVLRSKISGKILDAITW 300

Query: 301 TLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGAGSR 360
           TLI LL+GAFLWL+KTLLLKILASKFH ++FFDRIQE IFHHHVLQTL     +    S 
Sbjct: 301 TLITLLIGAFLWLIKTLLLKILASKFHMNRFFDRIQESIFHHHVLQTLLNPSPIGMPEST 360

Query: 361 NKFNCCRFSLESKKSDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSISQTLDES 420
            + N  R S + KKSDHKKVI +G IH+L+REKV+AWTMKVL++AV SSEMSISQ LDES
Sbjct: 361 VESNSGRLSFKGKKSDHKKVIDLGKIHQLKREKVSAWTMKVLVDAVASSEMSISQLLDES 420

Query: 421 YHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLVLPHFEVDE 480
           Y  +ADGEI DEME A   A+KI +N+A+ GN FI+EEDL+R M+KEEVDLVLP FEVDE
Sbjct: 421 Y-QVADGEIADEMEVAGYAAYKIFDNIAVPGNDFIEEEDLRRVMIKEEVDLVLPLFEVDE 480

Query: 481 TRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAVIWLLLMEIA 540
           TRRID K+LT WV+KVY+ERKTL HAL DTKTAVKQLNNLVTA+LIIVTA+IWLLLMEIA
Sbjct: 481 TRRIDWKSLTNWVLKVYKERKTLAHALKDTKTAVKQLNNLVTALLIIVTAIIWLLLMEIA 540

Query: 541 TSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVHLLVEEMNIL 600
           T+KVLVFLLSQLAVA FMFG++CKT FEAL+FVFVMHPFDVGDRCAV+GV L+VEEMNIL
Sbjct: 541 TTKVLVFLLSQLAVAVFMFGNTCKTIFEALVFVFVMHPFDVGDRCAVEGVPLMVEEMNIL 600

Query: 601 TTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIGVMKEKIKRY 660
           TTVFLKLNNE VYYPNSVLATK ITNYYRSPDM DT+EFSI FATPLERIG+MK++IKRY
Sbjct: 601 TTVFLKLNNEKVYYPNSVLATKSITNYYRSPDMSDTVEFSIGFATPLERIGLMKDRIKRY 660

Query: 661 LEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELVMELKRIFEE 720
           LEKNPQHWHPNHSVVVKEIE+VNK+  ALY+NHTMNFQDW+EK+RRR+ELVMELKRIFEE
Sbjct: 661 LEKNPQHWHPNHSVVVKEIEDVNKIKFALYSNHTMNFQDWTEKNRRRTELVMELKRIFEE 720

Query: 721 LKINYDLLPQTVHLFPVQE 738
           LKINY+LLPQTVHLFP +E
Sbjct: 721 LKINYNLLPQTVHLFPTEE 725

BLAST of HG10014285 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 630.2 bits (1624), Expect = 2.1e-180
Identity = 384/743 (51.68%), Postives = 503/743 (67.70%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKEPTDSCIAHGHDSNLPP 76
           S N    GG VV+ +    +S+  ++    + P+  +    +K P+         S L  
Sbjct: 6   SSNGGGGGGDVVINVPVEEASRRSKE---MASPESEKGVPFSKSPSPEI------SKLVG 65

Query: 77  TANKPPEIPSSKGT-LTSTRSLKRSIPSRPKSRFGEQPRFIDSNMFEENHLSLREQTGA- 136
           + NKPP  P+     LT  +S  RS+ S+PKSRF +    +D+++ EE    +REQ GA 
Sbjct: 66  SPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAG 125

Query: 137 -------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIK 196
                         S  S AP T      E+D++E+     K +++++   K+    LI+
Sbjct: 126 FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMR--SKISTLALIE 185

Query: 197 LVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIEG 256
              FV I+  LVASLT+  LK+  FWGL VWKWC+L  VI  GM+ T+W M ++V LIE 
Sbjct: 186 SAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIET 245

Query: 257 NFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLTL 316
           NFLL++KVLYFVHGLKKSVQV IWL L+LV W+ LF   NH         K+L  IT TL
Sbjct: 246 NFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTL 305

Query: 317 IALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGA---GS 376
           I++L GAF WLVKTLLLKILA+ F+ + FFDRIQ+ +FH +VLQTLS  PLME A   G 
Sbjct: 306 ISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGR 365

Query: 377 RNKFNCCRFSLESKKS--DHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS-ISQT 436
                   F+   KK     KKVI MG +H+++REKV+AWTM+VL+EAV +S +S IS T
Sbjct: 366 EPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 425

Query: 437 LDESYH----NIADGEITDEMETASAVAHKILENVALGNM-FIQEEDLKRFMVKEEVDLV 496
           LDE+ +      AD EIT EME A A A+ +  NVA     +I+EEDL RFM+KEEVDLV
Sbjct: 426 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 485

Query: 497 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 556
            P F+   ET RI  KA T+WVVKVY  R+ L H+LNDTKTAVKQLN LVTAIL++VT V
Sbjct: 486 FPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVV 545

Query: 557 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 616
           IWLLL+E+AT+KVL+F  +QL   AF+ GS+CK  FE+++FVFVMHP+DVGDRC VDGV 
Sbjct: 546 IWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVA 605

Query: 617 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 676
           +LVEEMN+LTTVFLKLNNE VYYPN+VLATKPI+NY+RSP+M +T+EFSISF+TP+ +I 
Sbjct: 606 MLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIA 665

Query: 677 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
            +KE+I  YLE+NPQHW P HSVVVKEIEN+NK+ +ALY++HT+ FQ+  E++ RR+EL 
Sbjct: 666 HLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELS 725

BLAST of HG10014285 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 630.2 bits (1624), Expect = 2.1e-180
Identity = 384/743 (51.68%), Postives = 503/743 (67.70%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKEPTDSCIAHGHDSNLPP 76
           S N    GG VV+ +    +S+  ++    + P+  +    +K P+         S L  
Sbjct: 6   SSNGGGGGGDVVINVPVEEASRRSKE---MASPESEKGVPFSKSPSPEI------SKLVG 65

Query: 77  TANKPPEIPSSKGT-LTSTRSLKRSIPSRPKSRFGEQPRFIDSNMFEENHLSLREQTGA- 136
           + NKPP  P+     LT  +S  RS+ S+PKSRF +    +D+++ EE    +REQ GA 
Sbjct: 66  SPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAG 125

Query: 137 -------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIK 196
                         S  S AP T      E+D++E+     K +++++   K+    LI+
Sbjct: 126 FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMR--SKISTLALIE 185

Query: 197 LVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIEG 256
              FV I+  LVASLT+  LK+  FWGL VWKWC+L  VI  GM+ T+W M ++V LIE 
Sbjct: 186 SAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIET 245

Query: 257 NFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLTL 316
           NFLL++KVLYFVHGLKKSVQV IWL L+LV W+ LF   NH         K+L  IT TL
Sbjct: 246 NFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTL 305

Query: 317 IALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGA---GS 376
           I++L GAF WLVKTLLLKILA+ F+ + FFDRIQ+ +FH +VLQTLS  PLME A   G 
Sbjct: 306 ISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGR 365

Query: 377 RNKFNCCRFSLESKKS--DHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS-ISQT 436
                   F+   KK     KKVI MG +H+++REKV+AWTM+VL+EAV +S +S IS T
Sbjct: 366 EPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 425

Query: 437 LDESYH----NIADGEITDEMETASAVAHKILENVALGNM-FIQEEDLKRFMVKEEVDLV 496
           LDE+ +      AD EIT EME A A A+ +  NVA     +I+EEDL RFM+KEEVDLV
Sbjct: 426 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 485

Query: 497 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 556
            P F+   ET RI  KA T+WVVKVY  R+ L H+LNDTKTAVKQLN LVTAIL++VT V
Sbjct: 486 FPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVV 545

Query: 557 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 616
           IWLLL+E+AT+KVL+F  +QL   AF+ GS+CK  FE+++FVFVMHP+DVGDRC VDGV 
Sbjct: 546 IWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVA 605

Query: 617 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 676
           +LVEEMN+LTTVFLKLNNE VYYPN+VLATKPI+NY+RSP+M +T+EFSISF+TP+ +I 
Sbjct: 606 MLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIA 665

Query: 677 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
            +KE+I  YLE+NPQHW P HSVVVKEIEN+NK+ +ALY++HT+ FQ+  E++ RR+EL 
Sbjct: 666 HLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELS 725

BLAST of HG10014285 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 630.2 bits (1624), Expect = 2.1e-180
Identity = 384/743 (51.68%), Postives = 503/743 (67.70%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDDNGYSVPKQNRVDSQTKEPTDSCIAHGHDSNLPP 76
           S N    GG VV+ +    +S+  ++    + P+  +    +K P+         S L  
Sbjct: 6   SSNGGGGGGDVVINVPVEEASRRSKE---MASPESEKGVPFSKSPSPEI------SKLVG 65

Query: 77  TANKPPEIPSSKGT-LTSTRSLKRSIPSRPKSRFGEQPRFIDSNMFEENHLSLREQTGA- 136
           + NKPP  P+     LT  +S  RS+ S+PKSRF +    +D+++ EE    +REQ GA 
Sbjct: 66  SPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEE---EVREQLGAG 125

Query: 137 -------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIK 196
                         S  S AP T      E+D++E+     K +++++   K+    LI+
Sbjct: 126 FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYKKVKLNREMR--SKISTLALIE 185

Query: 197 LVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIVVSLIEG 256
              FV I+  LVASLT+  LK+  FWGL VWKWC+L  VI  GM+ T+W M ++V LIE 
Sbjct: 186 SAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIET 245

Query: 257 NFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILDTITLTL 316
           NFLL++KVLYFVHGLKKSVQV IWL L+LV W+ LF   NH         K+L  IT TL
Sbjct: 246 NFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLF---NHDVKRSPAATKVLKCITRTL 305

Query: 317 IALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEGA---GS 376
           I++L GAF WLVKTLLLKILA+ F+ + FFDRIQ+ +FH +VLQTLS  PLME A   G 
Sbjct: 306 ISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGR 365

Query: 377 RNKFNCCRFSLESKKS--DHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS-ISQT 436
                   F+   KK     KKVI MG +H+++REKV+AWTM+VL+EAV +S +S IS T
Sbjct: 366 EPSTGHLSFATVVKKGTVKEKKVIDMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDT 425

Query: 437 LDESYH----NIADGEITDEMETASAVAHKILENVALGNM-FIQEEDLKRFMVKEEVDLV 496
           LDE+ +      AD EIT EME A A A+ +  NVA     +I+EEDL RFM+KEEVDLV
Sbjct: 426 LDETAYGEGKEQADREITSEME-ALAAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLV 485

Query: 497 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 556
            P F+   ET RI  KA T+WVVKVY  R+ L H+LNDTKTAVKQLN LVTAIL++VT V
Sbjct: 486 FPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVV 545

Query: 557 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 616
           IWLLL+E+AT+KVL+F  +QL   AF+ GS+CK  FE+++FVFVMHP+DVGDRC VDGV 
Sbjct: 546 IWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVA 605

Query: 617 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 676
           +LVEEMN+LTTVFLKLNNE VYYPN+VLATKPI+NY+RSP+M +T+EFSISF+TP+ +I 
Sbjct: 606 MLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRSPNMGETVEFSISFSTPVSKIA 665

Query: 677 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
            +KE+I  YLE+NPQHW P HSVVVKEIEN+NK+ +ALY++HT+ FQ+  E++ RR+EL 
Sbjct: 666 HLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQENRERNLRRTELS 725

BLAST of HG10014285 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 586.6 bits (1511), Expect = 2.7e-167
Identity = 363/745 (48.72%), Postives = 490/745 (65.77%), Query Frame = 0

Query: 17  SQNESENGGQVVVEISSVVSSKEIRDD---NGYSVPKQNRVDSQTKEPTDSCIAHGHDSN 76
           ++    NG +VV+ +S    SK+ R     N  + P  +    ++K      I       
Sbjct: 2   AERRVSNGEEVVINVSDKEDSKDPRASPSFNPLASPDSDAGIEKSKPVPPISIPTPEIYK 61

Query: 77  LPPTANKPPEIPSSKGTLTSTRSLKRSIPSRPKSRFGEQPRF-IDSNMFEENHLSLREQT 136
              + +KPP+IPS +G L   +SL RSI S+PKSRFGEQ  F  DS   E    SLREQ 
Sbjct: 62  FSGSVHKPPKIPSPEG-LVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQF 121

Query: 137 GA---------------TSSRSSAPSTTKAQPEEEDDEEKTEPLNKKHKKVKKVKKVKVK 196
           GA                S+RS A +      EEE DE   E + KK  K+ +VK+  +K
Sbjct: 122 GAGSFARGSFDRASPNNKSNRSVASAALSKVAEEEPDE--NEEIYKK-VKLHRVKRSGMK 181

Query: 197 TL--IKLVGFVFIIGCLVASLTVKRLKNCFFWGLRVWKWCLLATVILCGMIFTHWVMHIV 256
            L  ++LV F+ I+G L+ SLT+  +     WGL  WKWC+L  V L GM+ T+W MH V
Sbjct: 182 PLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWFMHFV 241

Query: 257 VSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALVLVTWVSLFDRRNHRNSNLGIIGKILD 316
           V +IE N+LL+KKVLYFVHGLKK+VQV IW +LVL+ W+ LFD    R        + LD
Sbjct: 242 VFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRK---TKRFLD 301

Query: 317 TITLTLIALLVGAFLWLVKTLLLKILASKFHKDQFFDRIQEFIFHHHVLQTLSRSPLMEG 376
            IT T+++LLVG+ L+LVKT  LK+LASKF+   FF+RIQE +FH +VLQTLS  PL+E 
Sbjct: 302 FITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIEE 361

Query: 377 AGSRNKFNCCRFSLESKKSD----HKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMS 436
           A +  +         ++  D     KKVI MG +HR+++EKV+AWTM+VLIEAV +S +S
Sbjct: 362 AENVGRVPSTGHLSFTRTKDGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAVGTSGIS 421

Query: 437 -ISQTLDE--SYHNIADGEITDEMETASAVAHKILENVALGN-MFIQEEDLKRFMVKEEV 496
            IS TLDE  +     D EIT+EME A A A+ +  NVA  N  +I+E+DL RFM+KEEV
Sbjct: 422 TISSTLDEVNNKKERTDKEITNEME-AVAAAYDVFNNVAKPNHNYIEEDDLLRFMIKEEV 481

Query: 497 DLVLPHFEVDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVT 556
           DLVLP  E  +T +I  K  T+WVV VY  RKT+ H+LNDTKTAVKQL+ L+T IL ++T
Sbjct: 482 DLVLPLIEDADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLITGILTVIT 541

Query: 557 AVIWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDG 616
            ++W++L++IA++K+L+   SQ    AFM GS+CK  FE+ +FVFVMHP+DVGDRC VDG
Sbjct: 542 FIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVGDRCVVDG 601

Query: 617 VHLLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLER 676
           V LLVEE+++LTTVFLK++NE V+YPNSVL +KPI+N+YRSPDM D ++F I+F+TP E+
Sbjct: 602 VMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIAFSTPAEK 661

Query: 677 IGVMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSE 733
           IG +K KI  YL  N QHW+P   V+V+ IEN+NK+ + +   HT+NFQ + EKS RR+ 
Sbjct: 662 IGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVEKSLRRTA 721

BLAST of HG10014285 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 418.7 bits (1075), Expect = 9.7e-117
Identity = 256/623 (41.09%), Postives = 371/623 (59.55%), Query Frame = 0

Query: 150 EEEDDEEKTEPLNKKHKKVKKVKKVKVKTLIKLVGFVFIIGCLVASLTVKRLKNCFFWGL 209
           EEEDD    E L ++++K     K+ +  +++ +  + II   V +L +  L+    W L
Sbjct: 218 EEEDDPFAAEDLPEEYRK----DKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWEL 277

Query: 210 RVWKWCLLATVILCGMIFTHWVMHIVVSLIEGNFLLKKKVLYFVHGLKKSVQVTIWLALV 269
           ++WKW  +  V++CG + + W++ IVV  IE NFLL+K+VLYFV+G++K+VQ  +WL LV
Sbjct: 278 QLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLV 337

Query: 270 LVTWVSLFDRRNHRNSNLGIIGKILDTITLTLIALLVGAFLWLVKTLLLKILASKFHKDQ 329
           L+ W  LFD +  + +N     K L  +T   + LLVG  LWLVKTLL+K+LAS FH   
Sbjct: 338 LLAWHFLFDEKVAKAANT----KALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMST 397

Query: 330 FFDRIQEFIFHHHVLQTLSRSPLMEGAGSRNKFNCCRFSLESKK---------------- 389
           +FDRIQE +F  +V++TLS  PL+E    +N+    R S+E KK                
Sbjct: 398 YFDRIQESLFTQYVIETLSGPPLIE--IQKNEEEEERISVEVKKFQNPGGVEIQSGAQKS 457

Query: 390 --------------------SDHKKVIGMGGIHRLQREKVTAWTMKVLIEAVISSEMSI- 449
                                   K I +  +H+L  + V+AW MK L+  + +  ++  
Sbjct: 458 PMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTL 517

Query: 450 -SQTLDESYHNIADGEITDEMETASAVAHKILENVAL-GNMFIQEEDLKRFMVKEEVDLV 509
             Q  D S  +    +I  E E A   A KI  NVA  G+ FI   D+ RF+  +E    
Sbjct: 518 DEQLQDPSLDDDKGNQIRSEFE-AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKT 577

Query: 510 LPHFE-VDETRRIDEKALTKWVVKVYQERKTLVHALNDTKTAVKQLNNLVTAILIIVTAV 569
           L  FE   ET RI + +L  WVV  ++ER+ L   LNDTKTAV +L+ +V  ++ I+  V
Sbjct: 578 LSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILV 637

Query: 570 IWLLLMEIATSKVLVFLLSQLAVAAFMFGSSCKTAFEALIFVFVMHPFDVGDRCAVDGVH 629
           IWL+++ I ++K LV + SQ+ V AF+FG+ CK  FE++I++FV+HPFDVGDRC +DGV 
Sbjct: 638 IWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQ 697

Query: 630 LLVEEMNILTTVFLKLNNEMVYYPNSVLATKPITNYYRSPDMCDTIEFSISFATPLERIG 689
           ++VEEMNILTTVFL+ +N+ V YPNS+L TK I NYYRSPDM D IEFSI   TP E+I 
Sbjct: 698 MVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKII 757

Query: 690 VMKEKIKRYLEKNPQHWHPNHSVVVKEIENVNKVTIALYTNHTMNFQDWSEKSRRRSELV 733
           ++K++I  Y+E    HW+P   +V K++E++N V IA++  H MN QD  EK  RRS+LV
Sbjct: 758 LIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLV 817