HG10014222 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014222 vs. NCBI nr
Match: XP_038896145.1 (amino acid transporter AVT6A-like [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.7e-229
Identity = 421/434 (97.00%), Postives = 429/434 (98.85%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTILVSADRKYRRSP+KPLLP+KCDHQE VEVGLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTILVSADRKYRRSPKKPLLPEKCDHQEAVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
           AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSV+YAGAVG+SLGSAGRTLLQVCI
Sbjct: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVSYAGAVGDSLGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL
Sbjct: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLM+GSIGMPRLMPKV DQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMNGSIGMPRLMPKVTDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVPILVTAYIC HNVHPIENELKDPTQMKSIVRTSLTICSTVYI TSFFGFLLFGDQTLD
Sbjct: 241 TVPILVTAYICQHNVHPIENELKDPTQMKSIVRTSLTICSTVYITTSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLN+DGLLFPYAIPI+FDN
Sbjct: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNIDGLLFPYAIPISFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSIT ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD
Sbjct: 361 RRFFSITTALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAVLS
Sbjct: 421 RLIAWIMFLLAVLS 434

BLAST of HG10014222 vs. NCBI nr
Match: XP_008452683.1 (PREDICTED: sodium-coupled neutral amino acid transporter 2-like [Cucumis melo] >KAA0064473.1 sodium-coupled neutral amino acid transporter 2-like [Cucumis melo var. makuwa] >TYK20115.1 sodium-coupled neutral amino acid transporter 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 790.4 bits (2040), Expect = 9.0e-225
Identity = 414/434 (95.39%), Postives = 422/434 (97.24%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTILVSADRKYRRSP+KPLLP+K  HQE VEVGLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTILVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
            VKQLGLIPGL+LIMLGSTLTELSIDFILKFSRASKSVTYAGAVGES GSAGRTLLQVCI
Sbjct: 61  VVKQLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVY+IIIGDV SGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTL IFAPL
Sbjct: 121 VVNNLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PKVIDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVP+LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD
Sbjct: 241 TVPVLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPI FDN
Sbjct: 301 DVLANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKD
Sbjct: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAV S
Sbjct: 421 RLIAWIMFLLAVFS 434

BLAST of HG10014222 vs. NCBI nr
Match: XP_004141301.1 (amino acid transporter AVT6A isoform X1 [Cucumis sativus] >KGN55300.1 hypothetical protein Csa_012788 [Cucumis sativus])

HSP 1 Score: 787.7 bits (2033), Expect = 5.8e-224
Identity = 411/434 (94.70%), Postives = 422/434 (97.24%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MT+LVSADRKYRRSP+K LLP+K DHQE VE GLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTMLVSADRKYRRSPKKLLLPEKYDHQEPVEAGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
            VKQLGLIPGL+LIMLGSTLTELSIDFILKFSRASKSVTYAGAVGES G+AGRTLLQVCI
Sbjct: 61  VVKQLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGNAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVYMIIIGDV SGTSANNIHHKGVMEEWFGQHWWTSR+SLMLLTTL IFAPL
Sbjct: 121 VVNNLGMLVVYMIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRLSLMLLTTLFIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRLMPKV+DQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLMPKVVDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVP+LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD
Sbjct: 241 TVPVLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN
Sbjct: 301 DVLANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFF ITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAAL+LRDTCGIASKKD
Sbjct: 361 RRFFLITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALILRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAV S
Sbjct: 421 RLIAWIMFLLAVFS 434

BLAST of HG10014222 vs. NCBI nr
Match: XP_023535239.1 (amino acid transporter AVT6A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.4 bits (2001), Expect = 3.0e-220
Identity = 400/439 (91.12%), Postives = 425/439 (96.81%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTI+V+ADRKYRRSPRK LLP+K DHQE +EVGL+GASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTIIVAADRKYRRSPRKSLLPEKYDHQEPLEVGLEGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
           AVKQLGLIPGL++I+LGSTLTELSIDFILKFSR SKS TYAGAVG+SLGSAGRTLLQVCI
Sbjct: 61  AVKQLGLIPGLIMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGML+VYMIIIGDVFSGT+ NN+HHKGVMEEWFGQHWWTSRISLML TTLLIFAPL
Sbjct: 121 VVNNLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP++IDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVPILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICS++YIATSFFGF+LFGDQTLD
Sbjct: 241 TVPILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFP AIPI F+N
Sbjct: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSIT+ALMSFIF+GANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCG+ASKKD
Sbjct: 361 RRFFSITVALMSFIFVGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGVASKKD 420

Query: 421 RVIAWIMFLLAVLSKEESI 440
           RVIAW+MFLLAVLS   +I
Sbjct: 421 RVIAWLMFLLAVLSSMAAI 439

BLAST of HG10014222 vs. NCBI nr
Match: KAG6591444.1 (Amino acid transporter AVT6A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-218
Identity = 401/439 (91.34%), Postives = 424/439 (96.58%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTI+V+ADRKYRRSPRK LLP+K DHQE +E GL+GASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTIIVAADRKYRRSPRKSLLPEKYDHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
           AVKQLGLIPGL++I+LGSTLTELSIDFILKFSR SKS TYAGAVG+SLGSAGRTLLQVCI
Sbjct: 61  AVKQLGLIPGLIMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGML+VYMIIIGDVFSGT+ NN+HHKGVMEEWFGQHWWTSRISLML TTLLIFAPL
Sbjct: 121 VVNNLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP++IDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVPILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICS++YIATSFFGF+LFGDQTLD
Sbjct: 241 TVPILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFP AIPI F+N
Sbjct: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD
Sbjct: 361 RRFFSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLSKEESI 440
           RVIAW+MFLLAVLS   +I
Sbjct: 421 RVIAWLMFLLAVLSSMAAI 438

BLAST of HG10014222 vs. ExPASy Swiss-Prot
Match: Q9LI61 (Amino acid transporter AVT6A OS=Arabidopsis thaliana OX=3702 GN=AVT6A PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.1e-140
Identity = 251/422 (59.48%), Postives = 333/422 (78.91%), Query Frame = 0

Query: 18  PLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLG 77
           PLLP+ C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ +I++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 78  STLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGD 137
           + LT+ SI+F+L+FS+A K+ +Y G +G S G+ GR LLQV ++VNN+G+L+VYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 138 VFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 197
           V +G + + IHH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 198 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHP 257
            LA+VF+ ITAG++I KL+ G + MPRL+P V D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 258 IENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 317
           I+NEL+DP+Q++ +VR++L +CS+VYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 318 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIG 377
           L+D VRVSY +HLMLVFPIVF+ LR+N+DGLLFP A  ++  N RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 378 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEE 437
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D  +A  M +LAVLS   
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 438 SI 440
           +I
Sbjct: 445 AI 445

BLAST of HG10014222 vs. ExPASy Swiss-Prot
Match: F4KBM7 (Amino acid transporter AVT6B OS=Arabidopsis thaliana OX=3702 GN=AVT6B PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.4e-134
Identity = 240/407 (58.97%), Postives = 319/407 (78.38%), Query Frame = 0

Query: 35  DGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRA 94
           +GASFSGAVFNL++TI+GAGIMALPA +K LGLIPG+ +I+L + LT+ SI+F+L+FS  
Sbjct: 33  NGASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNI 92

Query: 95  SKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVME 154
               +Y G + +S G  GR +LQV I+V+N+G+L+VYMIIIGDV +G +   IHH G++E
Sbjct: 93  GNQRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIGDVLAGKNEYGIHHAGMLE 152

Query: 155 EWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAK 214
            WFG  WW  R  ++L+TTL +FAPL  FKR+DSLR+TSA+SV LA+VF+ ITAG+ I K
Sbjct: 153 GWFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIK 212

Query: 215 LMDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRT 274
           L    + MPRL+P V D +SFW+LFT VP+LV AYICH+NVH I+NEL+DP+++K +VR+
Sbjct: 213 LFTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRS 272

Query: 275 SLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVF 334
           +L +CS+VY+ TS FG+LLFGD TLDDVLANFD DLG+P+ S+L+D VR SY  HLMLVF
Sbjct: 273 ALAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVF 332

Query: 335 PIVFFSLRLNVDGLLFPYAIPITF--DNRRFFSITIALMSFIFIGANFVPSIWDAFQLTG 394
           P+VF+ LR+N+DGL+FP A P+T    + RF SIT  L++ IF+GANF+PSIWDAFQ TG
Sbjct: 333 PVVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTG 392

Query: 395 ATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEESI 440
           ATAA+ +GFIFPAA++L+D    A+K+D+ IA  M +LAV S   +I
Sbjct: 393 ATAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAI 439

BLAST of HG10014222 vs. ExPASy Swiss-Prot
Match: Q0WQJ3 (Amino acid transporter AVT6D OS=Arabidopsis thaliana OX=3702 GN=AVT6D PE=2 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 6.7e-98
Identity = 196/430 (45.58%), Postives = 287/430 (66.74%), Query Frame = 0

Query: 16  RKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIM 75
           + PLLP++       E   +  SF+GAVFN+S++IVGAGIMA+PAA K LG+IP L +I+
Sbjct: 6   KAPLLPNQEPSSSSSE---NHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLSIIV 65

Query: 76  LGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIII 135
           + + L+ +S  F++K S A +S TYAG + ES G +G   + V  +V   G ++++ III
Sbjct: 66  IIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFSIII 125

Query: 136 GDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSAL 195
           GDV SG   + I H G+++EWFG HWW +R   +L   + +F PL+  +RV+ L ++SA+
Sbjct: 126 GDVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAFSSAI 185

Query: 196 SVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQA-SFWQLFTTVPILVTAYICHHN 255
           S  LA++FV I++ +AI  L+ G    PRL P++ D   SF+ LFT  P++VTA+  H N
Sbjct: 186 SFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFTFHFN 245

Query: 256 VHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPY 315
           VHP+  ELKDP  + S  R S+ +C+T+Y AT  F +LLFGD T+ DVL NFD       
Sbjct: 246 VHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQSTSSSV 305

Query: 316 SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFI 375
            SLL+D+VR+SY IHLMLVFP++ FSLR N+D LLFP  + +  DN+RFF++T  L+   
Sbjct: 306 GSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPLLISC 365

Query: 376 FIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLS 435
           F+GA  +P IW  FQ  G+T+ +S+ FIFPAA+VLR+  G ++ +++++A +M +LAV +
Sbjct: 366 FLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVLAVAT 425

Query: 436 KEESIILIPT 445
              SII I T
Sbjct: 426 ---SIIAIST 429

BLAST of HG10014222 vs. ExPASy Swiss-Prot
Match: Q9LYM2 (Amino acid transporter AVT6C OS=Arabidopsis thaliana OX=3702 GN=AVT6C PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 8.2e-96
Identity = 184/409 (44.99%), Postives = 275/409 (67.24%), Query Frame = 0

Query: 36  GASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRAS 95
           G+S SG VFN+S++I+GAGIM++PAA K LG++P  ++I + + L+ +S+ F++K + A 
Sbjct: 22  GSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLSTISVGFLMKSTLAG 81

Query: 96  KSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEE 155
           +S TYAG + ES G  G   +Q+  +V   G ++++ IIIGDV SG       H GV++E
Sbjct: 82  ESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIGDVLSGNENGGPEHIGVLQE 141

Query: 156 WFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKL 215
           WFG +WW +RI  +L     +  PL+  +RV+ L  +SA+S  LA++FV I++ +AI+ L
Sbjct: 142 WFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFLLAVLFVVISSVLAISAL 201

Query: 216 MDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRTS 275
           ++G    PRL P++ +  SFWQLFT  P++VTA+  H NVHPI  ELKDP Q+    + S
Sbjct: 202 VNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPIGFELKDPLQVIPATKIS 261

Query: 276 LTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFP 335
           + +C+ +Y AT  FG+LLFGD T+ D+L NFD   G    SLL+D+VR+SY +HLMLVFP
Sbjct: 262 VILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLLNDIVRLSYVLHLMLVFP 321

Query: 336 IVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIGANFVPSIWDAFQLTGATA 395
           ++ FSLR N+D LL+P    +  D +RF  +T+AL+   F+ A  VP IW  FQ  G+T 
Sbjct: 322 LLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSAIAVPDIWYFFQFMGSTI 381

Query: 396 AISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEESIILIPT 445
            +S+ FIFPAA+VLR+  G+++ +++++A IM +LAV +   SII I T
Sbjct: 382 TVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVAT---SIIAIST 427

BLAST of HG10014222 vs. ExPASy Swiss-Prot
Match: Q9M8L9 (Amino acid transporter AVT6E OS=Arabidopsis thaliana OX=3702 GN=AVT6E PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.1e-89
Identity = 179/402 (44.53%), Postives = 268/402 (66.67%), Query Frame = 0

Query: 36  GASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRAS 95
           G+   GAVFNL+++I+GAGIMALPA +K LGL+ G VLI+L + L+E+S++ +++FS   
Sbjct: 74  GSGIYGAVFNLTTSIIGAGIMALPATMKVLGLVLGFVLIILMALLSEISVELLVRFSVLY 133

Query: 96  KSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEE 155
           KS +Y   V  +LG   R L ++CI+VNN G+LVVY+II+GDV SG    ++HH GV+++
Sbjct: 134 KSKSYGEVVQFALGKTARVLSEICIIVNNGGVLVVYLIIMGDVMSG----SLHHIGVLDQ 193

Query: 156 WFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKL 215
           W G  +W  R  L+L+  ++  APL +  ++DSL  TSA SV LA+VFV +   VA  KL
Sbjct: 194 WLGNGFWDHRKVLILIVMVIFLAPLCALNKIDSLSVTSAASVALAVVFVVVCFVVATIKL 253

Query: 216 MDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHPIENEL--KDPTQMKSIVR 275
           ++G+I  PRL P    + +   L   +PI+  AY+CH NV PI NEL  + P +M  + R
Sbjct: 254 IEGTIDPPRLSPDFGSKQAILDLLVVIPIMSNAYVCHFNVQPIYNELEGRSPHKMNRVGR 313

Query: 276 TSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLV 335
            +  IC  VY +T+  G+LLFG  T  D+L NFD DLG+ +SS ++ +VR+ Y +HL+LV
Sbjct: 314 ITTAICVVVYASTAVSGYLLFGKDTESDILTNFDQDLGIRFSSAVNYIVRIGYILHLVLV 373

Query: 336 FPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIGANFVPSIWDAFQLTGA 395
           FP++ FSLR  V+ LLF  + P++   +R   +T+ L++ I+IG+  +P+IW AF+ TGA
Sbjct: 374 FPVIHFSLRETVNTLLFEGSPPLSESKKRSLGLTVVLLALIYIGSTMIPNIWTAFKFTGA 433

Query: 396 TAAISVGFIFPAALVLR--DTCGIASKKDRVIAWIMFLLAVL 434
           T+A+S+GF FPA + LR        S  +R ++W+M +LAV+
Sbjct: 434 TSAVSLGFTFPALIALRLGKQSNSLSFVERSVSWLMLILAVV 471

BLAST of HG10014222 vs. ExPASy TrEMBL
Match: A0A5A7V8L7 (Sodium-coupled neutral amino acid transporter 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002400 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.4e-225
Identity = 414/434 (95.39%), Postives = 422/434 (97.24%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTILVSADRKYRRSP+KPLLP+K  HQE VEVGLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTILVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
            VKQLGLIPGL+LIMLGSTLTELSIDFILKFSRASKSVTYAGAVGES GSAGRTLLQVCI
Sbjct: 61  VVKQLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVY+IIIGDV SGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTL IFAPL
Sbjct: 121 VVNNLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PKVIDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVP+LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD
Sbjct: 241 TVPVLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPI FDN
Sbjct: 301 DVLANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKD
Sbjct: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAV S
Sbjct: 421 RLIAWIMFLLAVFS 434

BLAST of HG10014222 vs. ExPASy TrEMBL
Match: A0A1S3BTU4 (sodium-coupled neutral amino acid transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103493628 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.4e-225
Identity = 414/434 (95.39%), Postives = 422/434 (97.24%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTILVSADRKYRRSP+KPLLP+K  HQE VEVGLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTILVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
            VKQLGLIPGL+LIMLGSTLTELSIDFILKFSRASKSVTYAGAVGES GSAGRTLLQVCI
Sbjct: 61  VVKQLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVY+IIIGDV SGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTL IFAPL
Sbjct: 121 VVNNLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PKVIDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVP+LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD
Sbjct: 241 TVPVLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPI FDN
Sbjct: 301 DVLANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKD
Sbjct: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAV S
Sbjct: 421 RLIAWIMFLLAVFS 434

BLAST of HG10014222 vs. ExPASy TrEMBL
Match: A0A0A0L004 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G645260 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.8e-224
Identity = 411/434 (94.70%), Postives = 422/434 (97.24%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MT+LVSADRKYRRSP+K LLP+K DHQE VE GLDGASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTMLVSADRKYRRSPKKLLLPEKYDHQEPVEAGLDGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
            VKQLGLIPGL+LIMLGSTLTELSIDFILKFSRASKSVTYAGAVGES G+AGRTLLQVCI
Sbjct: 61  VVKQLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGNAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGMLVVYMIIIGDV SGTSANNIHHKGVMEEWFGQHWWTSR+SLMLLTTL IFAPL
Sbjct: 121 VVNNLGMLVVYMIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRLSLMLLTTLFIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRLMPKV+DQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLMPKVVDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVP+LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD
Sbjct: 241 TVPVLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN
Sbjct: 301 DVLANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFF ITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAAL+LRDTCGIASKKD
Sbjct: 361 RRFFLITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALILRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLS 435
           R+IAWIMFLLAV S
Sbjct: 421 RLIAWIMFLLAVFS 434

BLAST of HG10014222 vs. ExPASy TrEMBL
Match: A0A6J1FER3 (amino acid transporter AVT6A-like OS=Cucurbita moschata OX=3662 GN=LOC111443403 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 4.0e-218
Identity = 400/439 (91.12%), Postives = 423/439 (96.36%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTI+V+ADRKYRRSPRK LLP+K DHQE +E GL+GASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTIIVAADRKYRRSPRKSLLPEKYDHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
           AVKQLGLIPGL++I+LGSTLTELSIDFILKFSR SKS TYAGAVG+SLGSAGRTLLQVCI
Sbjct: 61  AVKQLGLIPGLLMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGML+VYMIIIGDVFSGT+ NN+HHKGVMEEWFGQHWWTSRISLML TTLLIFAPL
Sbjct: 121 VVNNLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP++IDQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVPILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICS++YIATSFFGF+LFGDQTLD
Sbjct: 241 TVPILVTAYICHHNVLPIENELKDPTQMKSIVRTSLRICSSLYIATSFFGFILFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFP AIPI F+N
Sbjct: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIAS KD
Sbjct: 361 RRFFSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASNKD 420

Query: 421 RVIAWIMFLLAVLSKEESI 440
           RVIAW+MFLLAVLS   +I
Sbjct: 421 RVIAWLMFLLAVLSSTAAI 438

BLAST of HG10014222 vs. ExPASy TrEMBL
Match: A0A6J1IHA5 (amino acid transporter AVT6A-like OS=Cucurbita maxima OX=3661 GN=LOC111477216 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 8.2e-216
Identity = 397/439 (90.43%), Postives = 419/439 (95.44%), Query Frame = 0

Query: 1   MTILVSADRKYRRSPRKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPA 60
           MTI+V+ADRKYRRSPRK LLP+K  HQE +E GL+GASFSGAVFNLSSTIVGAGIMALPA
Sbjct: 1   MTIIVAADRKYRRSPRKSLLPEKYAHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPA 60

Query: 61  AVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCI 120
           AVKQLGLIPGLV+I+LGSTLTELSIDFILKFSR SKS TYAGAV +SLGS GRTLLQVCI
Sbjct: 61  AVKQLGLIPGLVMIILGSTLTELSIDFILKFSRTSKSATYAGAVSDSLGSVGRTLLQVCI 120

Query: 121 VVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPL 180
           VVNNLGML+VYMIIIGDVFSGT+ NN+HHKGVMEEWFGQHWWTSR SLML TTLLIFAPL
Sbjct: 121 VVNNLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRTSLMLFTTLLIFAPL 180

Query: 181 ISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFT 240
           ISFKRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP++ DQASFWQLFT
Sbjct: 181 ISFKRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELFDQASFWQLFT 240

Query: 241 TVPILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLD 300
           TVPILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICS++YIATSFFGF+LFGDQTLD
Sbjct: 241 TVPILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLD 300

Query: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDN 360
           DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFP AIPI F+N
Sbjct: 301 DVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNN 360

Query: 361 RRFFSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420
           RRFFSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD
Sbjct: 361 RRFFSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKD 420

Query: 421 RVIAWIMFLLAVLSKEESI 440
           RVIAW+MFLLAVLS   +I
Sbjct: 421 RVIAWLMFLLAVLSSMAAI 438

BLAST of HG10014222 vs. TAIR 10
Match: AT3G30390.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 501.5 bits (1290), Expect = 7.8e-142
Identity = 251/422 (59.48%), Postives = 333/422 (78.91%), Query Frame = 0

Query: 18  PLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLG 77
           PLLP+ C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ +I++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 78  STLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGD 137
           + LT+ SI+F+L+FS+A K+ +Y G +G S G+ GR LLQV ++VNN+G+L+VYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 138 VFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 197
           V +G + + IHH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 198 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHP 257
            LA+VF+ ITAG++I KL+ G + MPRL+P V D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 258 IENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 317
           I+NEL+DP+Q++ +VR++L +CS+VYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 318 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIG 377
           L+D VRVSY +HLMLVFPIVF+ LR+N+DGLLFP A  ++  N RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 378 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEE 437
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D  +A  M +LAVLS   
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 438 SI 440
           +I
Sbjct: 445 AI 445

BLAST of HG10014222 vs. TAIR 10
Match: AT3G30390.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 501.5 bits (1290), Expect = 7.8e-142
Identity = 251/422 (59.48%), Postives = 333/422 (78.91%), Query Frame = 0

Query: 18  PLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLG 77
           PLLP+ C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ +I++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 78  STLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGD 137
           + LT+ SI+F+L+FS+A K+ +Y G +G S G+ GR LLQV ++VNN+G+L+VYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 138 VFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 197
           V +G + + IHH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 198 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHP 257
            LA+VF+ ITAG++I KL+ G + MPRL+P V D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 258 IENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 317
           I+NEL+DP+Q++ +VR++L +CS+VYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 318 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIG 377
           L+D VRVSY +HLMLVFPIVF+ LR+N+DGLLFP A  ++  N RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 378 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEE 437
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D  +A  M +LAVLS   
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 438 SI 440
           +I
Sbjct: 445 AI 445

BLAST of HG10014222 vs. TAIR 10
Match: AT5G38820.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.4e-135
Identity = 240/407 (58.97%), Postives = 319/407 (78.38%), Query Frame = 0

Query: 35  DGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRA 94
           +GASFSGAVFNL++TI+GAGIMALPA +K LGLIPG+ +I+L + LT+ SI+F+L+FS  
Sbjct: 33  NGASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNI 92

Query: 95  SKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVME 154
               +Y G + +S G  GR +LQV I+V+N+G+L+VYMIIIGDV +G +   IHH G++E
Sbjct: 93  GNQRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIGDVLAGKNEYGIHHAGMLE 152

Query: 155 EWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAK 214
            WFG  WW  R  ++L+TTL +FAPL  FKR+DSLR+TSA+SV LA+VF+ ITAG+ I K
Sbjct: 153 GWFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIK 212

Query: 215 LMDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRT 274
           L    + MPRL+P V D +SFW+LFT VP+LV AYICH+NVH I+NEL+DP+++K +VR+
Sbjct: 213 LFTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRS 272

Query: 275 SLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVF 334
           +L +CS+VY+ TS FG+LLFGD TLDDVLANFD DLG+P+ S+L+D VR SY  HLMLVF
Sbjct: 273 ALAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVF 332

Query: 335 PIVFFSLRLNVDGLLFPYAIPITF--DNRRFFSITIALMSFIFIGANFVPSIWDAFQLTG 394
           P+VF+ LR+N+DGL+FP A P+T    + RF SIT  L++ IF+GANF+PSIWDAFQ TG
Sbjct: 333 PVVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTG 392

Query: 395 ATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEESI 440
           ATAA+ +GFIFPAA++L+D    A+K+D+ IA  M +LAV S   +I
Sbjct: 393 ATAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAI 439

BLAST of HG10014222 vs. TAIR 10
Match: AT2G40420.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 359.4 bits (921), Expect = 4.8e-99
Identity = 196/430 (45.58%), Postives = 287/430 (66.74%), Query Frame = 0

Query: 16  RKPLLPDKCDHQERVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIM 75
           + PLLP++       E   +  SF+GAVFN+S++IVGAGIMA+PAA K LG+IP L +I+
Sbjct: 6   KAPLLPNQEPSSSSSE---NHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLSIIV 65

Query: 76  LGSTLTELSIDFILKFSRASKSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIII 135
           + + L+ +S  F++K S A +S TYAG + ES G +G   + V  +V   G ++++ III
Sbjct: 66  IIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFSIII 125

Query: 136 GDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSAL 195
           GDV SG   + I H G+++EWFG HWW +R   +L   + +F PL+  +RV+ L ++SA+
Sbjct: 126 GDVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAFSSAI 185

Query: 196 SVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKVIDQA-SFWQLFTTVPILVTAYICHHN 255
           S  LA++FV I++ +AI  L+ G    PRL P++ D   SF+ LFT  P++VTA+  H N
Sbjct: 186 SFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFTFHFN 245

Query: 256 VHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPY 315
           VHP+  ELKDP  + S  R S+ +C+T+Y AT  F +LLFGD T+ DVL NFD       
Sbjct: 246 VHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQSTSSSV 305

Query: 316 SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFI 375
            SLL+D+VR+SY IHLMLVFP++ FSLR N+D LLFP  + +  DN+RFF++T  L+   
Sbjct: 306 GSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPLLISC 365

Query: 376 FIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLS 435
           F+GA  +P IW  FQ  G+T+ +S+ FIFPAA+VLR+  G ++ +++++A +M +LAV +
Sbjct: 366 FLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVLAVAT 425

Query: 436 KEESIILIPT 445
              SII I T
Sbjct: 426 ---SIIAIST 429

BLAST of HG10014222 vs. TAIR 10
Match: AT3G56200.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 352.4 bits (903), Expect = 5.8e-97
Identity = 184/409 (44.99%), Postives = 275/409 (67.24%), Query Frame = 0

Query: 36  GASFSGAVFNLSSTIVGAGIMALPAAVKQLGLIPGLVLIMLGSTLTELSIDFILKFSRAS 95
           G+S SG VFN+S++I+GAGIM++PAA K LG++P  ++I + + L+ +S+ F++K + A 
Sbjct: 22  GSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIAWLSTISVGFLMKSTLAG 81

Query: 96  KSVTYAGAVGESLGSAGRTLLQVCIVVNNLGMLVVYMIIIGDVFSGTSANNIHHKGVMEE 155
           +S TYAG + ES G  G   +Q+  +V   G ++++ IIIGDV SG       H GV++E
Sbjct: 82  ESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIGDVLSGNENGGPEHIGVLQE 141

Query: 156 WFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKL 215
           WFG +WW +RI  +L     +  PL+  +RV+ L  +SA+S  LA++FV I++ +AI+ L
Sbjct: 142 WFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFLLAVLFVVISSVLAISAL 201

Query: 216 MDGSIGMPRLMPKVIDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRTS 275
           ++G    PRL P++ +  SFWQLFT  P++VTA+  H NVHPI  ELKDP Q+    + S
Sbjct: 202 VNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPIGFELKDPLQVIPATKIS 261

Query: 276 LTICSTVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFP 335
           + +C+ +Y AT  FG+LLFGD T+ D+L NFD   G    SLL+D+VR+SY +HLMLVFP
Sbjct: 262 VILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLLNDIVRLSYVLHLMLVFP 321

Query: 336 IVFFSLRLNVDGLLFPYAIPITFDNRRFFSITIALMSFIFIGANFVPSIWDAFQLTGATA 395
           ++ FSLR N+D LL+P    +  D +RF  +T+AL+   F+ A  VP IW  FQ  G+T 
Sbjct: 322 LLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSAIAVPDIWYFFQFMGSTI 381

Query: 396 AISVGFIFPAALVLRDTCGIASKKDRVIAWIMFLLAVLSKEESIILIPT 445
            +S+ FIFPAA+VLR+  G+++ +++++A IM +LAV +   SII I T
Sbjct: 382 TVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVAT---SIIAIST 427