HG10014175 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014175
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytochrome P450
Location	Chr02: 8185546 .. 8189025 (-)
RNA-Seq Expression	HG10014175
Synteny	HG10014175

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAAGAGACTTCTTTCTTGTTCTCTCATTTCAACATCCCTCTCTTCGCCCTTCTCTTAACTACTTTCATTCTCCTTTCCAAATTTCGAAAAACAGACCACAGAAAACTCCCACCAGGCCCACCTTCTCTTCCGCTAATCGGTCACCTCCATCTTCTCAAAGAACCGTTCCACCAAATGTTGCAACATCTCTCGCAAAAATACGGTCCTGTCTTCTCACTCACCCTCGGTTTCCGCCCCGTGATGGTTGTATCCTCCCCCTCCGCTGTCCAAGAATGCTTCACAAAAAATGACATCGTTTTCGCCAGCCGCCCCCGTATGCTCTCCGGAAAGATCCTGAATTACGATTACACAGCGGTTGGTGCGACGGCGTATGGGCAGCATTGGCGGATCATGCGGCGCATAGCTACTACAGAATTGCTCTCCACTCACCGTCTCAATAGCTTGAGTAAAATCCGAGAGGAGGAAGTCAAATTGTGGGTGAAAAGTTTGTACGAAATGACGGAACAGGGGAAAAGTAATAAAGGAGTTGGAGTGGGGATAAGGTCGAAATTGACGGAACTGTCCTTCAACATTGCGTTGATGTTAATGGCGGGGAGACGGTATTTTGGTGGGGAAGTGGAGGGGGAGAGTGAGGAGGCGAGGGAGTTGAAGGAGACGATGAAGCAAGTTCCTATGCTGAGTGGAGCGTCGTATCCGGCGGATTTTTTGTCGGTCTTAAAACTGGTTGATTTTCAAGGATTTAGGAAGAGGCTGGCTAAAGTGAGAGTGGATGGAGATGCTTTTGCGCAAAGTCTTGTTGATGAACGCCGGAAAATGAAGGGTTCATCTGACCGAAAGACCATGATTGATACTCTGTTGTTGTTGCAGGAGTCGGACCCTGAGTATTACAAGGACGATATTATTAAGGGACAATTCTTGGTAAGCTAAATTATCAAATATATATTCCCATAAACGCCATAATACAAAACGAAATTCTCACGTATAAAAAAATATCAAAATATTTATCGAAAATAACAAAAAAAAAAAAATAATAATAATATTGATAGACATTACATCTATCACAACAATAAATTTTTTTTTTGTTATTTTGTGTAAATAGTTTTCATTATTTTTATATTTTAAAAAATTCTCCTAATACAAATACTTATTATATTTTCAACCACCAATATTTGGATGCATCCTAAAATACATCTGTCCTTATAAATCCTACTTTCACACACACACATATATATATATATATATATATATATATATATATATATATATAAACAATAAATTAAAATATTACAAAAAGAAAAAAATAAGTGTAATCCAAAATACATCTAATATTCTTTTTTCAACAGGCAACCCAAGAATATTACTTAAAAGAGAAAATTTTATGTGTGGAGGGTTGGAATGTGTATTGCATGCCCTCCATGTGACATAGTCTTTTTTTTTTTTTTTTTTCTTGCTTAAATCATAAACATGATTTGATTTCAGGCCATGTTTACTTTGTTGTAATCAAAATTATTGCAATTGTAATGATTTTTCTTTATTATTCATAATTCACACAATATTGTTAACTAATTTTTTTGAAAACATTTTTAAATGACCTATTTTTCATATTACCATTGATTGATTATGTTTACTGGAAAATAAACATGACCTTAGTGAGTTGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACGGACACAACTTGGGAAATTATAAAAAATAACATGTAGAAGTTTTCATCTTATAAAACTAGCACTCTTTTTGAAAAATCGTTCAAATAGTTTTTCTCGGTCCATCTCGAGCAAGATCGACAATCGAGATTAGTTAAAAAATTAACTTTTTCTAACTTCGATTATTTAGAGCCTTCTCGATGACATCTCGGGCAGTATTACCATCGAGATTCTATCAAAATTTTTCAAATAGTTAGAAAAACTATTTGAACGATTTTTCAAAAAAGGTGTTAGTTTTGTTTATGAAAATTTCAACGTGTTATTTTTTACCATTTCAAATATTATATTGTTTTTGAAGTTTGAGTTTGATAACATTTATGTCCATAAATTTTCATACTCAATTTTTATATTTATGAGGTTTGGAAAAAGTTTATTTAGTTCAAATGTTACGAGTTTTTTGCATTTTTCTCCCTTCTTTTCGAGCAAGAGTTTTTTTTTTCTATAGCTAATGAAAAATAACATGATTATTTTCTATTAAAAAATACATTTATTTTTAAAATAAAAATATTTTCCCTCATTCTTTCTTCTTCTCATCCGAAAAGAAGAAATAAAAAAGAAGAAAAAAAGAAAATAGTGGCGACGTCAAGTGGCCATGGAGGGAAGAACTGGGAAGAGAGAAAAAGAAAAAGAAAAAGAAAAATATTAATTAAAAGAACATTTTTTAATAAAAATGAGTGACACATTGATTTCAGTCAAACAAAAAACTAACCGCGTGGACTAATTAAACCATTTTCTCAAACCTCAATGTTAGAAAACTCAAGAACACAAAAATTACTATCAAACCCAAATCTCAAATATCAAAGTTGTATTTTTTTAAAGAATATATTAGACAGTACATATTTAAAAATCATAACAAAATTCAATTATAATTTAACATAAATTATTTTATCACAAAATATAAAAAACATGATTATCTACTTTAACCTTTAATCCGTGAAGCCATGCTTAGGTTGATTATTTATTGTTTATATAGAAATGTGTACTATATGTGGGTTGGTGGTGTGTACAACATATGTATATTAACACTGCTTTAACTTTGACAACAATTTACACGTGTGTTAATAATATTTATTTTTGATGATTTGAATACTAATTAAATGAAGGGATATGTGGTGTAGACTTTACTGGCTGCAGGAACTGATACTACAGCAGGAACATTAGAGTGGGCGATGTCGCTGCTGCTGAACCATCCAACAACAATGAAAAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTTGTACAGGAAGCTGATCTCAACAACCTACACTATCTTCAAGCCATAATCAATGAGACGTATCGCCTATTCCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCCGAAGATTGCACAATTGGAGGGTTCGATGTTCCAAAAGGAACCATGTTAGTGGTGAATGCTTGGGCTGTCCATCGAGACCCCAAGGTTTGGGAAAATCCCAATTGTTTCAAACCAGAGAGGTTTAAAGAAGGAGAGAGTGTGGAAGTAAGTAAATTGTTACCATTTGGAATGGGAAGAAGGGCATGTCCAGGGGCTGGCCTTGCCCATAGAGTGGTGGCTTTGGCTTTGGCTACTCTCATTCAATGCTTTGAGTGGGGGAGACTCGACCAAAAAGAAGTTGACTTGTCCCCAGGGATTGGACTCACCATGCCCAAAGCCACCCCTCTTGAGGCCATGTGCAAACCTCGTGCTTCCATCATTCATTTACTATCACAGCTTTGA

mRNA sequence

ATGGAAGAAGAGACTTCTTTCTTGTTCTCTCATTTCAACATCCCTCTCTTCGCCCTTCTCTTAACTACTTTCATTCTCCTTTCCAAATTTCGAAAAACAGACCACAGAAAACTCCCACCAGGCCCACCTTCTCTTCCGCTAATCGGTCACCTCCATCTTCTCAAAGAACCGTTCCACCAAATGTTGCAACATCTCTCGCAAAAATACGGTCCTGTCTTCTCACTCACCCTCGGTTTCCGCCCCGTGATGGTTGTATCCTCCCCCTCCGCTGTCCAAGAATGCTTCACAAAAAATGACATCGTTTTCGCCAGCCGCCCCCGTATGCTCTCCGGAAAGATCCTGAATTACGATTACACAGCGGTTGGTGCGACGGCGTATGGGCAGCATTGGCGGATCATGCGGCGCATAGCTACTACAGAATTGCTCTCCACTCACCGTCTCAATAGCTTGAGTAAAATCCGAGAGGAGGAAGTCAAATTGTGGGTGAAAAGTTTGTACGAAATGACGGAACAGGGGAAAAGTAATAAAGGAGTTGGAGTGGGGATAAGGTCGAAATTGACGGAACTGTCCTTCAACATTGCGTTGATGTTAATGGCGGGGAGACGGTATTTTGGTGGGGAAGTGGAGGGGGAGAGTGAGGAGGCGAGGGAGTTGAAGGAGACGATGAAGCAAGTTCCTATGCTGAGTGGAGCGTCGTATCCGGCGGATTTTTTGTCGGTCTTAAAACTGGTTGATTTTCAAGGATTTAGGAAGAGGCTGGCTAAAGTGAGAGTGGATGGAGATGCTTTTGCGCAAAGTCTTGTTGATGAACGCCGGAAAATGAAGGGTTCATCTGACCGAAAGACCATGATTGATACTCTGTTGTTGTTGCAGGAGTCGGACCCTGAGTATTACAAGGACGATATTATTAAGGGACAATTCTTGACTTTACTGGCTGCAGGAACTGATACTACAGCAGGAACATTAGAGTGGGCGATGTCGCTGCTGCTGAACCATCCAACAACAATGAAAAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTTGTACAGGAAGCTGATCTCAACAACCTACACTATCTTCAAGCCATAATCAATGAGACGTATCGCCTATTCCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCCGAAGATTGCACAATTGGAGGGTTCGATGTTCCAAAAGGAACCATGTTAGTGGTGAATGCTTGGGCTGTCCATCGAGACCCCAAGGTTTGGGAAAATCCCAATTGTTTCAAACCAGAGAGGTTTAAAGAAGGAGAGAGTGTGGAAGTAAGTAAATTGTTACCATTTGGAATGGGAAGAAGGGCATGTCCAGGGGCTGGCCTTGCCCATAGAGTGGTGGCTTTGGCTTTGGCTACTCTCATTCAATGCTTTGAGTGGGGGAGACTCGACCAAAAAGAAGTTGACTTGTCCCCAGGGATTGGACTCACCATGCCCAAAGCCACCCCTCTTGAGGCCATGTGCAAACCTCGTGCTTCCATCATTCATTTACTATCACAGCTTTGA

Coding sequence (CDS)

ATGGAAGAAGAGACTTCTTTCTTGTTCTCTCATTTCAACATCCCTCTCTTCGCCCTTCTCTTAACTACTTTCATTCTCCTTTCCAAATTTCGAAAAACAGACCACAGAAAACTCCCACCAGGCCCACCTTCTCTTCCGCTAATCGGTCACCTCCATCTTCTCAAAGAACCGTTCCACCAAATGTTGCAACATCTCTCGCAAAAATACGGTCCTGTCTTCTCACTCACCCTCGGTTTCCGCCCCGTGATGGTTGTATCCTCCCCCTCCGCTGTCCAAGAATGCTTCACAAAAAATGACATCGTTTTCGCCAGCCGCCCCCGTATGCTCTCCGGAAAGATCCTGAATTACGATTACACAGCGGTTGGTGCGACGGCGTATGGGCAGCATTGGCGGATCATGCGGCGCATAGCTACTACAGAATTGCTCTCCACTCACCGTCTCAATAGCTTGAGTAAAATCCGAGAGGAGGAAGTCAAATTGTGGGTGAAAAGTTTGTACGAAATGACGGAACAGGGGAAAAGTAATAAAGGAGTTGGAGTGGGGATAAGGTCGAAATTGACGGAACTGTCCTTCAACATTGCGTTGATGTTAATGGCGGGGAGACGGTATTTTGGTGGGGAAGTGGAGGGGGAGAGTGAGGAGGCGAGGGAGTTGAAGGAGACGATGAAGCAAGTTCCTATGCTGAGTGGAGCGTCGTATCCGGCGGATTTTTTGTCGGTCTTAAAACTGGTTGATTTTCAAGGATTTAGGAAGAGGCTGGCTAAAGTGAGAGTGGATGGAGATGCTTTTGCGCAAAGTCTTGTTGATGAACGCCGGAAAATGAAGGGTTCATCTGACCGAAAGACCATGATTGATACTCTGTTGTTGTTGCAGGAGTCGGACCCTGAGTATTACAAGGACGATATTATTAAGGGACAATTCTTGACTTTACTGGCTGCAGGAACTGATACTACAGCAGGAACATTAGAGTGGGCGATGTCGCTGCTGCTGAACCATCCAACAACAATGAAAAAGGCTTGGACAGAGATAAACCAGGTTGTTGGAGACCAAAGACTTGTACAGGAAGCTGATCTCAACAACCTACACTATCTTCAAGCCATAATCAATGAGACGTATCGCCTATTCCCAGCCCTTCCAATACTGGTGCCACGTGAATCATCCGAAGATTGCACAATTGGAGGGTTCGATGTTCCAAAAGGAACCATGTTAGTGGTGAATGCTTGGGCTGTCCATCGAGACCCCAAGGTTTGGGAAAATCCCAATTGTTTCAAACCAGAGAGGTTTAAAGAAGGAGAGAGTGTGGAAGTAAGTAAATTGTTACCATTTGGAATGGGAAGAAGGGCATGTCCAGGGGCTGGCCTTGCCCATAGAGTGGTGGCTTTGGCTTTGGCTACTCTCATTCAATGCTTTGAGTGGGGGAGACTCGACCAAAAAGAAGTTGACTTGTCCCCAGGGATTGGACTCACCATGCCCAAAGCCACCCCTCTTGAGGCCATGTGCAAACCTCGTGCTTCCATCATTCATTTACTATCACAGCTTTGA

Protein sequence

MEEETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL

Homology

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BLAST of HG10014175 vs. NCBI nr
Match: XP_004141394.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012467 [Cucumis sativus])

HSP 1 Score: 887.1 bits (2291), Expect = 7.2e-254
Identity = 446/523 (85.28%), Postives = 481/523 (91.97%), Query Frame = 0


Query: 1   MEEETSFLFS---HF---NIPLFALLLTTFILLSKFRKTDHRK-LPPGPPSLPLIGHLHL 60
           ME++TSF FS   HF   +IPL A+ LT+  L+SKFR+T H K LPP PPSLP+IGHLHL
Sbjct: 1   MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHLHL 60

Query: 61  LKEPFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKI 120
           LKEPFHQMLQ  S+KYGPVFSLTLGFRP++VVSSPSAVQECFTKNDIVFASRPRMLSGKI
Sbjct: 61  LKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGKI 120

Query: 121 LNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGK 180
           LNYDYTAVGATAYGQHWRIMRRIATTELLST RLN LSKIREEEVKLWVK LY    + +
Sbjct: 121 LNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRAE 180

Query: 181 SNKGVGVGIRSKLTELSFNIALMLMAGRRYFGGEVE-GESEEARELKETMKQVPMLSGAS 240
            NKG  VG+RSKLTELSFNIALMLMAGRRYFG E+E GESEEA+ELKETMKQVP+LSGAS
Sbjct: 181 VNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGAS 240

Query: 241 YPADFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQ 300
           YPADFLSVLKL+DFQGFRKRLAKVRVDGDAFAQSLVDERRK+KGS  +RKTMID++L LQ
Sbjct: 241 YPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCLQ 300

Query: 301 ESDPEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQR 360
           ESDPEYYKDDIIKGQFLTLLAAGTDTTA TLEWAMSLLLNHPT ++KAWTEINQVVGDQR
Sbjct: 301 ESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQR 360

Query: 361 LVQEADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVH 420
           L++EAD N LHYLQAIINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWAVH
Sbjct: 361 LIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVH 420

Query: 421 RDPKVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFE 480
           RDP VWE+PNCFKPERFK+GE+VEV+KLLPFGMGRRACPGAGLAHRVV+LALATLIQCFE
Sbjct: 421 RDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFE 480

Query: 481 WGRLD-QKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           WG+LD QK+VDL PGIGLTMPKA PLEAMCKPRAS+ HLLSQL
Sbjct: 481 WGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

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BLAST of HG10014175 vs. NCBI nr
Match: XP_008452612.1 (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa] >TYK20162.1 isoflavone 2'-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 877.1 bits (2265), Expect = 7.5e-251
Identity = 441/520 (84.81%), Postives = 474/520 (91.15%), Query Frame = 0


Query: 1   MEEETS--FLFSHFNIPLFALLLTTFILLSKFRKTDHRK--LPPGPPSLPLIGHLHLLKE 60
           ME++TS  F FSHFN PL A+ LTT  L+SKFR+++H K  LPP PPSLP+IGHLHLLKE
Sbjct: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKE 62

Query: 61  PFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNY 120
           PFHQMLQ LS KYGPVFSLTLGFRP++VVSSPSAV ECFTKNDIVFASRPRMLSGKILNY
Sbjct: 63  PFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNY 122

Query: 121 DYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNK 180
           DYTAVGATAYGQHWRIMRRIATTELLST RLN  SK+REEEVKLWVK LY      +  K
Sbjct: 123 DYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQK 182

Query: 181 GVGVGIRSKLTELSFNIALMLMAGRRYFGGEV-EGESEEARELKETMKQVPMLSGASYPA 240
           G  VG+RSKLTELSFNIALML AGRRYFG EV EGESEEA+ELKETMKQVP+LSGASYPA
Sbjct: 183 GGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPA 242

Query: 241 DFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQESD 300
           DFLSVLKL+DFQGFRKRLAKVRVDGDAFAQSLVDERR++KGS  +RKTMID +L LQESD
Sbjct: 243 DFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESD 302

Query: 301 PEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQ 360
           PEYYKDDIIKGQFLTLLAAGTDT A TLEWAMSLLLNHP  M KAWTEINQVVGDQRLV+
Sbjct: 303 PEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVR 362

Query: 361 EADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDP 420
           EAD NNLHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWAVHR+P
Sbjct: 363 EADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNP 422

Query: 421 KVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGR 480
           +VWE+PNCFKPERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV+LALATLIQCFEWG+
Sbjct: 423 EVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGK 482

Query: 481 LD-QKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           LD +K+VDL PGIGLTMPKA PLEAMCKPRAS+ HLLSQL
Sbjct: 483 LDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

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BLAST of HG10014175 vs. NCBI nr
Match: XP_038900261.1 (LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 847.8 bits (2189), Expect = 4.8e-242
Identity = 431/514 (83.85%), Postives = 458/514 (89.11%), Query Frame = 0


Query: 1   MEEETSFLFSHF-NIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFH 60
           MEE+ SF FSHF  IPLFALL+TT  LLSKFRKT+HRKLPP PPSLP+IGHLHLLKEPFH
Sbjct: 1   MEEQASFFFSHFPTIPLFALLITTLFLLSKFRKTEHRKLPPSPPSLPVIGHLHLLKEPFH 60

Query: 61  QMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYT 120
           QMLQ LS+KYGPVFSLTLGFRPV+VVS+PSAVQE                  KILNYDYT
Sbjct: 61  QMLQDLSKKYGPVFSLTLGFRPVVVVSAPSAVQE-----------------WKILNYDYT 120

Query: 121 AVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVG 180
           AVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVK LY MTE G+SNKGV 
Sbjct: 121 AVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKRLYGMTEVGESNKGVR 180

Query: 181 VGIRSKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLS 240
           VG+RSKLTELSFNIALMLM GRRYFGGEVEGESEEA+ELKETMKQVP+LSGASYPADFLS
Sbjct: 181 VGLRSKLTELSFNIALMLMTGRRYFGGEVEGESEEAKELKETMKQVPLLSGASYPADFLS 240

Query: 241 VLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYK 300
           VLK VDFQ        VRVDGDAFAQS+VDERRK+KGS +RKT+ID+LLLLQESDPEYYK
Sbjct: 241 VLKWVDFQ--------VRVDGDAFAQSVVDERRKLKGSCERKTIIDSLLLLQESDPEYYK 300

Query: 301 DDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLN 360
           D+IIKGQFLTLLAAGTDTTA TLEWAMSLLLNHPT MKKAWTEIN+VVGDQRLV+EAD N
Sbjct: 301 DEIIKGQFLTLLAAGTDTTARTLEWAMSLLLNHPTEMKKAWTEINEVVGDQRLVEEADRN 360

Query: 361 NLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWEN 420
             HYLQAIINETYRLFPALPIL PRESSEDCTIGGFD+PKGTMLVVNAWAVHRDP VWE+
Sbjct: 361 KPHYLQAIINETYRLFPALPILAPRESSEDCTIGGFDIPKGTMLVVNAWAVHRDPNVWEH 420

Query: 421 PNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKE 480
           PN F+PERFK G+ +EVSKLLPFGMGRRA PGAGLAHRVVALALATLIQCFEWGR DQK 
Sbjct: 421 PNSFQPERFKPGQXLEVSKLLPFGMGRRAGPGAGLAHRVVALALATLIQCFEWGRPDQKG 480

Query: 481 VDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           +DLSPGIGLTMP+A PLEA+CKPRASI HLLSQL
Sbjct: 481 LDLSPGIGLTMPRAIPLEAICKPRASITHLLSQL 489

[Close]

BLAST of HG10014175 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 844.0 bits (2179), Expect = 7.0e-241
Identity = 419/511 (82.00%), Postives = 462/511 (90.41%), Query Frame = 0


Query: 4   ETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQ 63
           E + LFS+F +PL  L+L   +L+ KFRK +H+ LPPGPPSLP+IGHLHLLKEPFHQMLQ
Sbjct: 6   EETLLFSNFEVPLLVLIL-ALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFHQMLQ 65

Query: 64  HLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGA 123
            LSQKYGP+ SL+LGFRPV+VVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGA
Sbjct: 66  DLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGA 125

Query: 124 TAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIR 183
           TAYGQHWRIMRRIATTELLST+RLN+L  IR++EVKLW+KSLY ++  GK+N  V V IR
Sbjct: 126 TAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRIS--GKNN--VRVEIR 185

Query: 184 SKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKL 243
           SKLTELSFNIALMLM G+RYFG E   +SEEARELK TMKQVP+LSGASYPADFLSVLKL
Sbjct: 186 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 245

Query: 244 VDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKG-SSDRKTMIDTLLLLQESDPEYYKDDI 303
           VD+QGFRKRLAKVRVDGDAFAQSL+DERR  K   ++RKTMID+LL +Q+ +PEYYKDDI
Sbjct: 246 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDI 305

Query: 304 IKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLH 363
           IKGQFLTLLAAGTDTTAGTLEWA+SLLLNHPT MKKAWTEIN+ VGD+RLV+E DLN LH
Sbjct: 306 IKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLH 365

Query: 364 YLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNC 423
           YLQ IINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWA+HRDPKVWE+PN 
Sbjct: 366 YLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNS 425

Query: 424 FKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDL 483
           F+PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW RLD KEVDL
Sbjct: 426 FRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNKEVDL 485

Query: 484 SPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           +PGIGLTMPKA PL AM KPRA IIHLLSQL
Sbjct: 486 TPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

[Close]

BLAST of HG10014175 vs. NCBI nr
Match: XP_023523499.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 843.2 bits (2177), Expect = 1.2e-240
Identity = 418/511 (81.80%), Postives = 461/511 (90.22%), Query Frame = 0


Query: 4   ETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQ 63
           E + LFS+F +PL  L+L   ++L KFRK DH+ LPPGPPSLP+IGHLHLLKEPFHQMLQ
Sbjct: 6   EETLLFSNFEVPLLVLILALLLIL-KFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQMLQ 65

Query: 64  HLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGA 123
            LS KYGP+ SL+LGFRPV+VVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGA
Sbjct: 66  DLSHKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGA 125

Query: 124 TAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIR 183
           TAYGQHWRIMRRIATTELLST+RLN+L  IR++EVKLW+KSLY ++  GK+N  V V IR
Sbjct: 126 TAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRIS--GKNN--VRVEIR 185

Query: 184 SKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKL 243
           SKLTELSFNIALMLM G+RYFG E   +SEEARELK TMKQVP+LSGASYPADFLSVLKL
Sbjct: 186 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 245

Query: 244 VDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKG-SSDRKTMIDTLLLLQESDPEYYKDDI 303
           VD+QGFRKRLAKVRVDGDAFAQSL+DERR  K   ++RKTMID+LL +Q+ +PEYYKDDI
Sbjct: 246 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDI 305

Query: 304 IKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLH 363
           IKGQFLTLLAAGTDTTAGTLEWA+SLLLNHPT MKKAWTEIN+ VGD+RLV+E DLN LH
Sbjct: 306 IKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLH 365

Query: 364 YLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNC 423
           YLQ IINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWA+HRDPKVWE+PN 
Sbjct: 366 YLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNS 425

Query: 424 FKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDL 483
           F+PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW RLD KEVDL
Sbjct: 426 FRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDSKEVDL 485

Query: 484 SPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           +PGIGLTMPKA+PL AM KPR  IIHLLSQL
Sbjct: 486 TPGIGLTMPKASPLVAMFKPRTPIIHLLSQL 511

[Close]

BLAST of HG10014175 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 264/507 (52.07%), Postives = 355/507 (70.02%), Query Frame = 0


Query: 3   EETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPP-SLPLIGHLHLLKEPFHQM 62
           E ++ L++   + L ++ L  F +  +      R LPP P  +LP+IGHLHL+ +  H+ 
Sbjct: 2   ESSTLLYTFLAVVLLSISLKLFPVSRR-----RRNLPPSPGLALPVIGHLHLIGKLLHRS 61

Query: 63  LQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAV 122
           L  LS+KYG VFSL LG R V+VVSSP+A +ECFTKNDIVFA+RP  + GK + Y+YT +
Sbjct: 62  LYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTM 121

Query: 123 GATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVG 182
             + YG+HWR +RR+A  E+ S   LN    IRE+EVK  + SLY+ + Q        V 
Sbjct: 122 VGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGK----VE 181

Query: 183 IRSKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVL 242
           ++SKL+ELSFN+ + ++AG+RYFG +V  +S+EA+  +  + +V   +GAS P DF+  L
Sbjct: 182 MKSKLSELSFNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFL 241

Query: 243 KLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDD 302
           + +DF+ + K+++K+  + DAF Q L+ E R  K   +  TMID LL LQES PEYY D 
Sbjct: 242 RWIDFKNYEKKVSKISQEMDAFLQRLIHESRINK---NNVTMIDHLLSLQESQPEYYTDQ 301

Query: 303 IIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNL 362
           IIKG  + LL AGTDT+A T+EWAMSLLLNHP T++KA TEI   VG  RL++E DL  L
Sbjct: 302 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKL 361

Query: 363 HYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPN 422
            YL  II+ET+RL PA P+LVP ESS+DC + G+DVPKGT+L+VNAWA+HRDP+ W+ P 
Sbjct: 362 TYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPT 421

Query: 423 CFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVD 482
            FKPER   G  +E SKL+PFGMGRR+CPG+GLA RVV L L  LIQCFEW R+ + ++D
Sbjct: 422 LFKPER-HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 481

Query: 483 LSPGIGLTMPKATPLEAMCKPRASIIH 509
           ++ G GLTMPKA PLEA+CKPR +I+H
Sbjct: 482 MAEGSGLTMPKAQPLEALCKPR-NILH 492

[Close]

BLAST of HG10014175 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 3.0e-130
Identity = 241/492 (48.98%), Postives = 326/492 (66.26%), Query Frame = 0


Query: 14  IPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQHLSQKYGPVF 73
           I LF L++T  +  +  RK   + LPPGP  LP+IG+LH LK+P H     LSQKYG +F
Sbjct: 10  ISLFFLIITLKVFFNTSRK--FKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIF 69

Query: 74  SLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIM 133
           SL  G R V+VVSS +  QECFTKNDIV A+RP  L+GK + Y+ T V  + YG HWR +
Sbjct: 70  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 129

Query: 134 RRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSFNI 193
           RRI + E+LS+HRLNS  +IR +E+   ++ L + +  G +     V +R   +E++FN 
Sbjct: 130 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE----VELRPMFSEMTFNT 189

Query: 194 ALMLMAGRRYFGGEVE-GESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRKR 253
            + +++G+RY+G + +  + EEAR  +  +K+V  L GA+   DFL  L+  DF G  KR
Sbjct: 190 IMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKR 249

Query: 254 LAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLLA 313
           L K+    DAF Q L+DE R  K +S+  TMID LL  Q+S PEYY D IIKG  + +L 
Sbjct: 250 LKKISKRTDAFLQGLIDEHRFGKRNSN--TMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 309

Query: 314 AGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINETY 373
           AGTDT++ T+EWAMS LLNHP  MKKA  E++  +G  R V E D++ L YLQ+I+ ET 
Sbjct: 310 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 369

Query: 374 RLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERF-KEG 433
           RL  A P+LVP  SSED ++GG+++P+ T+L+VNAW +HRDP +W +P CFKPERF KEG
Sbjct: 370 RLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG 429

Query: 434 ESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTMP 493
              EV+KLL FG+GRRACPG  L+ R   L L  LIQCFEW R+ ++++D+    G+T  
Sbjct: 430 ---EVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAG 489

Query: 494 KATPLEAMCKPR 504
           K T L AMCK R
Sbjct: 490 KKTSLNAMCKVR 490

[Close]

BLAST of HG10014175 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.3e-128
Identity = 233/491 (47.45%), Postives = 325/491 (66.19%), Query Frame = 0


Query: 17  FALLLTTFI----LLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQHLSQKYGPV 76
           ++LL  +FI    +L K      + LPPGPP++P+IG+LH LK P H+    LSQ YG +
Sbjct: 5   YSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDI 64

Query: 77  FSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRI 136
           FSL  G R V+VVSSPS   ECFTKNDI+ A+RPR L+GK + Y+YT +G+ +YG HWR 
Sbjct: 65  FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 124

Query: 137 MRRIATTELLSTHRLNSLSKIREEEV-KLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSF 196
           +RRI T ++LS +RLNS   +R +E  +L  K L ++  +G       V +R +LTE++F
Sbjct: 125 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFG--FTKVELRPRLTEMTF 184

Query: 197 NIALMLMAGRRYFGGEVE-GESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFR 256
           N  + +++G+RY+G + +  + EEA++ +E + ++  L GA+   DFL +L++VD     
Sbjct: 185 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 244

Query: 257 KRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTL 316
           KR  ++    +AF + L++E R+    SD  TMID LL L ES PEYY D +IKG    +
Sbjct: 245 KRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 304

Query: 317 LAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINE 376
           L AGTDT+A T+EW MS LLNHP  +KKA  E++  +G  +LV E DL+ L YLQ II+E
Sbjct: 305 LLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISE 364

Query: 377 TYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERFKE 436
           T RL P  P+L+P  SSEDCTIG F+VPK T+++ N W +HRDPK W +   FKPERF++
Sbjct: 365 TLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEK 424

Query: 437 GESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTM 496
            E  EV+K++ FG+GRRACPG  LA R V   +  LIQCFEW R  ++++D+  G G+TM
Sbjct: 425 EE--EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITM 484

Query: 497 PKATPLEAMCK 502
           P   PL AMCK
Sbjct: 485 PMKIPLRAMCK 491

[Close]

BLAST of HG10014175 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 459.9 bits (1182), Expect = 3.7e-128
Identity = 234/513 (45.61%), Postives = 337/513 (65.69%), Query Frame = 0


Query: 1   MEEETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQ 60
           ME  +   +S F + LF      FI     R    + LPPGPPSLP+IG+LH LK P H+
Sbjct: 1   MEILSLLSYSVFYLALF------FIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHR 60

Query: 61  MLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTA 120
             + LS+KYG VFSL  G R V+VVSS S  Q+CFTKND+V A+RPR LSGK + Y+YT 
Sbjct: 61  TFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTT 120

Query: 121 VGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGV 180
           +G+T+YG+HWR +RRI   ++LS HR+NS S IR +E +  +  L +      S     +
Sbjct: 121 LGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLAD----DSSTNFAEM 180

Query: 181 GIRSKLTELSFNIALMLMAGRRYFGGEVE-GESEEARELKETMKQVPMLSGASYPADFLS 240
            + S+L +++FN  + +++G+RY+G + +  + +EA + ++ + ++  LSGA+   DF+ 
Sbjct: 181 ELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMP 240

Query: 241 VLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYK 300
           +L+ +DF+   KRL  +    DAF + L++E R  K  ++  TMID LL LQ+S PEYY 
Sbjct: 241 LLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERAN--TMIDHLLNLQDSQPEYYT 300

Query: 301 DDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLN 360
           D IIKG  L +L AGTD++A TLEW+MS LLNHP  +KK   E++  VG  RLV E+DL 
Sbjct: 301 DQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLP 360

Query: 361 NLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWEN 420
            L YL+ +INET RL+   P+L+P  +S++C IGG+ VP+ T++++NAWA+HRDP++W  
Sbjct: 361 KLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTE 420

Query: 421 PNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKE 480
              FKPERF+  +  E+ KL+ FGMGRRACPG GLA R +++ LA LIQCF+W  ++  +
Sbjct: 421 ATTFKPERFE--KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDK 480

Query: 481 VDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQ 513
           +DL+   G T+ K  PL+AMCK R  I  +  Q
Sbjct: 481 IDLAERDGFTLTKLVPLKAMCKSRPVINKVFKQ 499

[Close]

BLAST of HG10014175 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 449.5 bits (1155), Expect = 5.0e-125
Identity = 227/501 (45.31%), Postives = 329/501 (65.67%), Query Frame = 0


Query: 17  FALLLTTFILLS---KFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQHLSQKYGPVF 76
           F LL   F+++S    + KT    LPPGPPS P +GHLHL+K P H++LQ  S +YGP+F
Sbjct: 4   FILLPLLFLVISYKFLYSKTQRFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPIF 63

Query: 77  SLTLGFRPVMVVSSPSAVQECFT-KNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRI 136
           SL  G R V+V++SPS  QE FT +NDIV +SRP  L+ K + Y++T VG   YG HWR 
Sbjct: 64  SLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRN 123

Query: 137 MRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTE-QGKSNKGVGVGIRSKLTELSF 196
           +RRI + E+LS+HRL +   IR++E+   +  L   T+   +SN    + +   L++L+F
Sbjct: 124 LRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLTF 183

Query: 197 NIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRK 256
           N  + ++ G+RY+G +V  + EEA   K+ +  + M SGA++ AD+L +LKL     F K
Sbjct: 184 NNIVRMVTGKRYYGDDVNNK-EEAELFKKLVYDIAMYSGANHSADYLPILKLFG-NKFEK 243

Query: 257 RLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLL 316
            +  +    D   Q L+DE R+ K   +  TM++ L+ LQ+  PEYY D IIKG  ++++
Sbjct: 244 EVKAIGKSMDDILQRLLDECRRDK---EGNTMVNHLISLQQQQPEYYTDVIIKGLMMSMM 303

Query: 317 AAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINET 376
            AGT+T+A TLEWAM+ LL +P  ++KA +EI++ +G  RL+ E+D+  L YLQ +++ET
Sbjct: 304 LAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSET 363

Query: 377 YRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERFKE- 436
           +RLFP  P L+PR  ++D  IGG+DVP+ T+++VNAWA+HRDP++WE P  F P+R+ + 
Sbjct: 364 FRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDG 423

Query: 437 -GESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLT 496
            G    V KL+PFG GRR CPGAGL  R+V LAL +LIQCFEW  +  +E+D+S   GL 
Sbjct: 424 CGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLG 483

Query: 497 MPKATPLEAMCKPRASIIHLL 511
           M K  PL AMC+PR  +  LL
Sbjct: 484 MRKMDPLRAMCRPRPIMSKLL 499

[Close]

BLAST of HG10014175 vs. ExPASy TrEMBL
Match: A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.5e-254
Identity = 446/523 (85.28%), Postives = 481/523 (91.97%), Query Frame = 0


Query: 1   MEEETSFLFS---HF---NIPLFALLLTTFILLSKFRKTDHRK-LPPGPPSLPLIGHLHL 60
           ME++TSF FS   HF   +IPL A+ LT+  L+SKFR+T H K LPP PPSLP+IGHLHL
Sbjct: 1   MEQQTSFFFSQDHHFHPISIPLLAIFLTSLFLISKFRRTQHTKLLPPSPPSLPVIGHLHL 60

Query: 61  LKEPFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKI 120
           LKEPFHQMLQ  S+KYGPVFSLTLGFRP++VVSSPSAVQECFTKNDIVFASRPRMLSGKI
Sbjct: 61  LKEPFHQMLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGKI 120

Query: 121 LNYDYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGK 180
           LNYDYTAVGATAYGQHWRIMRRIATTELLST RLN LSKIREEEVKLWVK LY    + +
Sbjct: 121 LNYDYTAVGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRAE 180

Query: 181 SNKGVGVGIRSKLTELSFNIALMLMAGRRYFGGEVE-GESEEARELKETMKQVPMLSGAS 240
            NKG  VG+RSKLTELSFNIALMLMAGRRYFG E+E GESEEA+ELKETMKQVP+LSGAS
Sbjct: 181 VNKGGRVGLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGAS 240

Query: 241 YPADFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQ 300
           YPADFLSVLKL+DFQGFRKRLAKVRVDGDAFAQSLVDERRK+KGS  +RKTMID++L LQ
Sbjct: 241 YPADFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWCERKTMIDSMLCLQ 300

Query: 301 ESDPEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQR 360
           ESDPEYYKDDIIKGQFLTLLAAGTDTTA TLEWAMSLLLNHPT ++KAWTEINQVVGDQR
Sbjct: 301 ESDPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQR 360

Query: 361 LVQEADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVH 420
           L++EAD N LHYLQAIINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWAVH
Sbjct: 361 LIREADTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVH 420

Query: 421 RDPKVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFE 480
           RDP VWE+PNCFKPERFK+GE+VEV+KLLPFGMGRRACPGAGLAHRVV+LALATLIQCFE
Sbjct: 421 RDPNVWEDPNCFKPERFKQGETVEVNKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFE 480

Query: 481 WGRLD-QKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           WG+LD QK+VDL PGIGLTMPKA PLEAMCKPRAS+ HLLSQL
Sbjct: 481 WGKLDNQKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 523

[Close]

BLAST of HG10014175 vs. ExPASy TrEMBL
Match: A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.6e-251
Identity = 441/520 (84.81%), Postives = 474/520 (91.15%), Query Frame = 0


Query: 1   MEEETS--FLFSHFNIPLFALLLTTFILLSKFRKTDHRK--LPPGPPSLPLIGHLHLLKE 60
           ME++TS  F FSHFN PL A+ LTT  L+SKFR+++H K  LPP PPSLP+IGHLHLLKE
Sbjct: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKE 62

Query: 61  PFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNY 120
           PFHQMLQ LS KYGPVFSLTLGFRP++VVSSPSAV ECFTKNDIVFASRPRMLSGKILNY
Sbjct: 63  PFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNY 122

Query: 121 DYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNK 180
           DYTAVGATAYGQHWRIMRRIATTELLST RLN  SK+REEEVKLWVK LY      +  K
Sbjct: 123 DYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQK 182

Query: 181 GVGVGIRSKLTELSFNIALMLMAGRRYFGGEV-EGESEEARELKETMKQVPMLSGASYPA 240
           G  VG+RSKLTELSFNIALML AGRRYFG EV EGESEEA+ELKETMKQVP+LSGASYPA
Sbjct: 183 GGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPA 242

Query: 241 DFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQESD 300
           DFLSVLKL+DFQGFRKRLAKVRVDGDAFAQSLVDERR++KGS  +RKTMID +L LQESD
Sbjct: 243 DFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESD 302

Query: 301 PEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQ 360
           PEYYKDDIIKGQFLTLLAAGTDT A TLEWAMSLLLNHP  M KAWTEINQVVGDQRLV+
Sbjct: 303 PEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVR 362

Query: 361 EADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDP 420
           EAD NNLHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWAVHR+P
Sbjct: 363 EADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNP 422

Query: 421 KVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGR 480
           +VWE+PNCFKPERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV+LALATLIQCFEWG+
Sbjct: 423 EVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGK 482

Query: 481 LD-QKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           LD +K+VDL PGIGLTMPKA PLEAMCKPRAS+ HLLSQL
Sbjct: 483 LDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

[Close]

BLAST of HG10014175 vs. ExPASy TrEMBL
Match: A0A1S3BTN1 (isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.6e-251
Identity = 441/520 (84.81%), Postives = 474/520 (91.15%), Query Frame = 0


Query: 1   MEEETS--FLFSHFNIPLFALLLTTFILLSKFRKTDHRK--LPPGPPSLPLIGHLHLLKE 60
           ME++TS  F FSHFN PL A+ LTT  L+SKFR+++H K  LPP PPSLP+IGHLHLLKE
Sbjct: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKE 62

Query: 61  PFHQMLQHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNY 120
           PFHQMLQ LS KYGPVFSLTLGFRP++VVSSPSAV ECFTKNDIVFASRPRMLSGKILNY
Sbjct: 63  PFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNY 122

Query: 121 DYTAVGATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNK 180
           DYTAVGATAYGQHWRIMRRIATTELLST RLN  SK+REEEVKLWVK LY      +  K
Sbjct: 123 DYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQK 182

Query: 181 GVGVGIRSKLTELSFNIALMLMAGRRYFGGEV-EGESEEARELKETMKQVPMLSGASYPA 240
           G  VG+RSKLTELSFNIALML AGRRYFG EV EGESEEA+ELKETMKQVP+LSGASYPA
Sbjct: 183 GGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPA 242

Query: 241 DFLSVLKLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQESD 300
           DFLSVLKL+DFQGFRKRLAKVRVDGDAFAQSLVDERR++KGS  +RKTMID +L LQESD
Sbjct: 243 DFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWCERKTMIDAMLSLQESD 302

Query: 301 PEYYKDDIIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQ 360
           PEYYKDDIIKGQFLTLLAAGTDT A TLEWAMSLLLNHP  M KAWTEINQVVGDQRLV+
Sbjct: 303 PEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGDQRLVR 362

Query: 361 EADLNNLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDP 420
           EAD NNLHYLQ+IINETYRLFPALPILVPRESSEDCTIGGFD+PKGTMLVVNAWAVHR+P
Sbjct: 363 EADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRNP 422

Query: 421 KVWENPNCFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGR 480
           +VWE+PNCFKPERFK+GE+VEVSKLLPFGMGRRACPGAGLAHRVV+LALATLIQCFEWG+
Sbjct: 423 EVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQCFEWGK 482

Query: 481 LD-QKEVDLSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           LD +K+VDL PGIGLTMPKA PLEAMCKPRAS+ HLLSQL
Sbjct: 483 LDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

[Close]

BLAST of HG10014175 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.4e-241
Identity = 419/511 (82.00%), Postives = 462/511 (90.41%), Query Frame = 0


Query: 4   ETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQ 63
           E + LFS+F +PL  L+L   +L+ KFRK +H+ LPPGPPSLP+IGHLHLLKEPFHQMLQ
Sbjct: 6   EETLLFSNFEVPLLVLIL-ALLLIFKFRKAEHKNLPPGPPSLPVIGHLHLLKEPFHQMLQ 65

Query: 64  HLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGA 123
            LSQKYGP+ SL+LGFRPV+VVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVGA
Sbjct: 66  DLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVGA 125

Query: 124 TAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIR 183
           TAYGQHWRIMRRIATTELLST+RLN+L  IR++EVKLW+KSLY ++  GK+N  V V IR
Sbjct: 126 TAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRIS--GKNN--VRVEIR 185

Query: 184 SKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKL 243
           SKLTELSFNIALMLM G+RYFG E   +SEEARELK TMKQVP+LSGASYPADFLSVLKL
Sbjct: 186 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 245

Query: 244 VDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKG-SSDRKTMIDTLLLLQESDPEYYKDDI 303
           VD+QGFRKRLAKVRVDGDAFAQSL+DERR  K   ++RKTMID+LL +Q+ +PEYYKDDI
Sbjct: 246 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKKADDNERKTMIDSLLSMQQMEPEYYKDDI 305

Query: 304 IKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLH 363
           IKGQFLTLLAAGTDTTAGTLEWA+SLLLNHPT MKKAWTEIN+ VGD+RLV+E DLN LH
Sbjct: 306 IKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQLH 365

Query: 364 YLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNC 423
           YLQ IINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWA+HRDPKVWE+PN 
Sbjct: 366 YLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWEDPNS 425

Query: 424 FKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDL 483
           F+PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW RLD KEVDL
Sbjct: 426 FRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNKEVDL 485

Query: 484 SPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           +PGIGLTMPKA PL AM KPRA IIHLLSQL
Sbjct: 486 TPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

[Close]

BLAST of HG10014175 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.4e-239
Identity = 416/512 (81.25%), Postives = 460/512 (89.84%), Query Frame = 0


Query: 3   EETSFLFSHFNIPLFALLLTTFILLSKFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQML 62
           EET  LFS+F + L  +L+   +++ KFRK DH+ LPPGPPSLP+IGHLHLLKEPFHQML
Sbjct: 6   EETLLLFSNFEV-LLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQML 65

Query: 63  QHLSQKYGPVFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVG 122
           Q LSQKYGP+ SL+LGFRPV+VVSSP+A QECF+KNDI+FASRPRM+SGKILNYDYTAVG
Sbjct: 66  QDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTAVG 125

Query: 123 ATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGI 182
           ATAYGQHWRIMRRIATTELLSTHRLN+L  IR++EVKLW+KSLY +T  GK+N  V V I
Sbjct: 126 ATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRIT--GKNN--VRVEI 185

Query: 183 RSKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLK 242
           RSKLTELSFNIALMLM G+RYFG E   +SEEARELK TMKQVP+LSGASYPADFLSVLK
Sbjct: 186 RSKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLK 245

Query: 243 LVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGS-SDRKTMIDTLLLLQESDPEYYKDD 302
           LVD+QGFRK+LAKVRVDGDAFAQSL+DERR  K   ++RKTMID+LL +Q+ +PEYYKD 
Sbjct: 246 LVDYQGFRKKLAKVRVDGDAFAQSLIDERRNKKADHNERKTMIDSLLSMQQMEPEYYKDH 305

Query: 303 IIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNL 362
           IIKGQFLTLLAAGTDTTAGTLEWA+SLLLNHPT MKKAWTEIN+ VGD+RLV+E DLN L
Sbjct: 306 IIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDLNQL 365

Query: 363 HYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPN 422
           HYLQ IINETYRLFPALPILVPRESSEDCTIGGFDVP GTMLVVNAWA+HRDPKVWE+PN
Sbjct: 366 HYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWEDPN 425

Query: 423 CFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVD 482
            F+PERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVV LALATLIQCFEW RLD KEVD
Sbjct: 426 SFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNKEVD 485

Query: 483 LSPGIGLTMPKATPLEAMCKPRASIIHLLSQL 514
           L+PGIGLTMPK++PL AM KPRA I HLLSQL
Sbjct: 486 LTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

[Close]

BLAST of HG10014175 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 460.3 bits (1183), Expect = 2.0e-129
Identity = 242/502 (48.21%), Postives = 339/502 (67.53%), Query Frame = 0


Query: 16  LFALLLTTFILLSKFRKTDHR-KLPPGPP-SLPLIGHLHLLKEPFHQMLQHLSQKY--GP 75
           +F++L     L+    K   +  LPP P  SLP+IGHL LLK P H+    LSQ     P
Sbjct: 7   IFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAP 66

Query: 76  VFSLTLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWR 135
           +FSL LG R V V SS S  +ECFTKND+V A+RP  +  K + YDYT + A +YG HWR
Sbjct: 67  IFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWR 126

Query: 136 IMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSF 195
            +RRI + E+ S HRLNS   IR++E++  V  L     +  S + V V ++S L++L+F
Sbjct: 127 NLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL----SRNFSQEFVKVDMKSMLSDLTF 186

Query: 196 NIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRK 255
           N  L ++AG+RY+G  VE +  EA+ +++ +  V   +GA    D+L VL+LV    +  
Sbjct: 187 NNILRMVAGKRYYGDGVE-DDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYET 246

Query: 256 RLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLL 315
           R+ K+    D F Q LVDE+R+ K   +  TMID LL LQES P+Y+ D IIKG  L L+
Sbjct: 247 RVKKLAGRLDEFLQGLVDEKREAKEKGN--TMIDHLLTLQESQPDYFTDRIIKGNMLALI 306

Query: 316 AAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINET 375
            AGTDT+A TLEWA+S +LNHP  + KA  EI++ +G  RL+ E+D++NL YLQ I++ET
Sbjct: 307 LAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSET 366

Query: 376 YRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERF-KE 435
            RL+PA P+L+P  +SEDC + G+D+P+GT+L+ N WA+HRDP++W++P  FKPERF KE
Sbjct: 367 LRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKE 426

Query: 436 GESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTM 495
           GE+    KL+PFG+GRRACPG+GLAHR++ L L +LIQC EW ++ + EVD+S G G+TM
Sbjct: 427 GEA---QKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE-EVDMSEGKGVTM 486

Query: 496 PKATPLEAMCKPRASIIHLLSQ 513
           PKA PLEAMC+ R S++ + ++
Sbjct: 487 PKAKPLEAMCRARPSVVKIFNE 495

[Close]

BLAST of HG10014175 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 458.0 bits (1177), Expect = 1.0e-128
Identity = 245/494 (49.60%), Postives = 331/494 (67.00%), Query Frame = 0


Query: 21  LTTFILLSKFRKTDHRKLPPGPP-SLPLIGHLHLLKEPFHQMLQHLSQKYG--PVFSLTL 80
           L  F+L    RK +   LPP P  SLP+IGHLHLLK P H+    LS+  G  PVF L L
Sbjct: 17  LAIFLLTRSKRKLN---LPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLRL 76

Query: 81  GFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRRIA 140
           G R V V+SS S  +ECFTKND+V A+RP+    K L Y+ T + + +YG HWR +RRIA
Sbjct: 77  GNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLRRIA 136

Query: 141 TTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSFNIALML 200
             E+ STHRLNS   IR++E++  +  L   +  G     V V +++ LT L+ N  + +
Sbjct: 137 AVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHG----FVEVEMKTLLTNLASNTTIRM 196

Query: 201 MAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRKRLAKVR 260
           +AG+RYFG     ++++A+ +K  + +    +GA  P D+LS+L+ V    + KR+  + 
Sbjct: 197 LAGKRYFG----EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLG 256

Query: 261 VDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLLAAGTDT 320
              D F Q LVDE+R  K   +  TMID LL LQ+  P+YY D IIKG  LTL+ AGTDT
Sbjct: 257 NRFDTFLQKLVDEKRAEKEKGE--TMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTDT 316

Query: 321 TAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINETYRLFPA 380
           ++ TLEWAMS LLNHP  +KKA  EI++ VG  RLV E+D+ NL YLQ+I+ ET R++PA
Sbjct: 317 SSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYPA 376

Query: 381 LPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERF-KEGESVEV 440
           +P+L+P  SSEDC +GG+D+P GTM++ NAWA+HRDP+VWE+P  FKPERF KEGE+   
Sbjct: 377 VPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEA--- 436

Query: 441 SKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTMPKATPL 500
            KL+ FGMGRRACPGAGLAHR++  AL +L+QCFEW R+ +  VD++   G T+PKA PL
Sbjct: 437 EKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAIPL 492

Query: 501 EAMCKPRASIIHLL 511
            AMCK R+ +  L+
Sbjct: 497 RAMCKARSIVDKLI 492

[Close]

BLAST of HG10014175 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.6e-126
Identity = 227/501 (45.31%), Postives = 329/501 (65.67%), Query Frame = 0


Query: 17  FALLLTTFILLS---KFRKTDHRKLPPGPPSLPLIGHLHLLKEPFHQMLQHLSQKYGPVF 76
           F LL   F+++S    + KT    LPPGPPS P +GHLHL+K P H++LQ  S +YGP+F
Sbjct: 4   FILLPLLFLVISYKFLYSKTQRFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGPIF 63

Query: 77  SLTLGFRPVMVVSSPSAVQECFT-KNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRI 136
           SL  G R V+V++SPS  QE FT +NDIV +SRP  L+ K + Y++T VG   YG HWR 
Sbjct: 64  SLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHWRN 123

Query: 137 MRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTE-QGKSNKGVGVGIRSKLTELSF 196
           +RRI + E+LS+HRL +   IR++E+   +  L   T+   +SN    + +   L++L+F
Sbjct: 124 LRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDLTF 183

Query: 197 NIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRK 256
           N  + ++ G+RY+G +V  + EEA   K+ +  + M SGA++ AD+L +LKL     F K
Sbjct: 184 NNIVRMVTGKRYYGDDVNNK-EEAELFKKLVYDIAMYSGANHSADYLPILKLFG-NKFEK 243

Query: 257 RLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLL 316
            +  +    D   Q L+DE R+ K   +  TM++ L+ LQ+  PEYY D IIKG  ++++
Sbjct: 244 EVKAIGKSMDDILQRLLDECRRDK---EGNTMVNHLISLQQQQPEYYTDVIIKGLMMSMM 303

Query: 317 AAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINET 376
            AGT+T+A TLEWAM+ LL +P  ++KA +EI++ +G  RL+ E+D+  L YLQ +++ET
Sbjct: 304 LAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSET 363

Query: 377 YRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERFKE- 436
           +RLFP  P L+PR  ++D  IGG+DVP+ T+++VNAWA+HRDP++WE P  F P+R+ + 
Sbjct: 364 FRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDG 423

Query: 437 -GESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLT 496
            G    V KL+PFG GRR CPGAGL  R+V LAL +LIQCFEW  +  +E+D+S   GL 
Sbjct: 424 CGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLG 483

Query: 497 MPKATPLEAMCKPRASIIHLL 511
           M K  PL AMC+PR  +  LL
Sbjct: 484 MRKMDPLRAMCRPRPIMSKLL 499

[Close]

BLAST of HG10014175 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 444.9 bits (1143), Expect = 8.8e-125
Identity = 228/489 (46.63%), Postives = 331/489 (67.69%), Query Frame = 0


Query: 19  LLLTTFILLSKFRKTDHRKLPPGPP-SLPLIGHLHLLKEPFHQMLQHLSQKYG--PVFSL 78
           ++L+   L+ + ++     LPP P  +LP+IGHL LLK P H++   +SQ  G  P+ SL
Sbjct: 13  IVLSLIFLIGRIKR--KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISL 72

Query: 79  TLGFRPVMVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTAVGATAYGQHWRIMRR 138
            LG R + VVSS S  +ECFTKND++ A+R   +S K ++Y  + V + +Y +HWR +RR
Sbjct: 73  RLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRR 132

Query: 139 IATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVGIRSKLTELSFNIAL 198
           I   E+ S HRLNS S IR +E++  +  L   +  G +     V ++S  ++L+FN  +
Sbjct: 133 IGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTK----VEMKSMFSDLTFNNII 192

Query: 199 MLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVLKLVDFQGFRKRLAK 258
            ++AG+ Y+G   E +  EA+ ++  + +    SG    AD++ +L  + +     R+ K
Sbjct: 193 RMLAGKCYYGDGKE-DDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKK 252

Query: 259 VRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDDIIKGQFLTLLAAGT 318
           +    D F Q LVDE+R+ K   +  TM+D LL LQE+ PEYY D IIKG  L+L+A GT
Sbjct: 253 LAGRLDEFLQGLVDEKREGKEKKE-NTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGT 312

Query: 319 DTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNLHYLQAIINETYRLF 378
           DTTA TLEWA+S LLN+P  + KA  EI++++G  RL++E+D+ NL YLQ I++ET RL+
Sbjct: 313 DTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLY 372

Query: 379 PALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPNCFKPERF-KEGESV 438
           PA P+L+P  +S+DC +GG+D+P+GTML+ NAWA+HRDP +W++P  FKPERF KEGE+ 
Sbjct: 373 PAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGEA- 432

Query: 439 EVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVDLSPGIGLTMPKAT 498
              KL+PFG+GRRACPG+GLA R+V L+L +LIQCFEW R+ ++EVD++ G GLTMPKA 
Sbjct: 433 --KKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKAR 488

Query: 499 PLEAMCKPR 504
           PLEAMC+ R
Sbjct: 493 PLEAMCRAR 488

[Close]

BLAST of HG10014175 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 235/510 (46.08%), Postives = 333/510 (65.29%), Query Frame = 0


Query: 10  SHFNIPLFALLLTTFILLS-KFRKTDHRKLPPGPPS-LPLIGHLHLLKEPFHQMLQHLSQ 69
           ++  + L+++    F+++S KF K   + LPP PP  LP+IGHL LLK P H+ L+  S+
Sbjct: 4   TNIRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSE 63

Query: 70  KY-----GPVFSLTLGFRPVMVVSSPS-AVQECFTKNDIVFASRPRMLSGKILNYDYTAV 129
                  G V SL LG R V VVSS   A +ECF KND+V A+RP+++ GK + Y+ T +
Sbjct: 64  TLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNM 123

Query: 130 GATAYGQHWRIMRRIATTELLSTHRLNSLSKIREEEVKLWVKSLYEMTEQGKSNKGVGVG 189
            A  YG HWR +RR+ T E+ STHRLN    +R +EV+  +  L  +       K   V 
Sbjct: 124 IAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLA----GTKKTVVE 183

Query: 190 IRSKLTELSFNIALMLMAGRRYFGGEVEGESEEARELKETMKQVPMLSGASYPADFLSVL 249
           ++  L +L+FN  + +M G+RY+G E   E EEA+ +++ +  V   + +    D++ +L
Sbjct: 184 LKPMLMDLTFNNIMRMMTGKRYYGEETTDE-EEAKRVRKLVADVGANTSSGNAVDYVPIL 243

Query: 250 KLVDFQGFRKRLAKVRVDGDAFAQSLVDERRKMKGSSDRKTMIDTLLLLQESDPEYYKDD 309
           +L  F  +  R+ K+  + D F Q L+D++R  + +    TMID LL+LQ+SD EYY D 
Sbjct: 244 RL--FSSYENRVKKLGEETDKFLQGLIDDKRGQQETG--TTMIDHLLVLQKSDIEYYTDQ 303

Query: 310 IIKGQFLTLLAAGTDTTAGTLEWAMSLLLNHPTTMKKAWTEINQVVGDQRLVQEADLNNL 369
           IIKG  L ++ AGT+T+A TLEWA+S LLNHP  + KA  EI+  VG  RL++EADL+ L
Sbjct: 304 IIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSEL 363

Query: 370 HYLQAIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAVHRDPKVWENPN 429
            YL+ I+ ET RL PA P+LVP  +SEDC IG +D+P+GT L+VNAWA+HRDP  W++P+
Sbjct: 364 PYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPD 423

Query: 430 CFKPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVALALATLIQCFEWGRLDQKEVD 489
            FKPERF++ E  E  KLL FG+GRRACPG+GLA R+V LAL +LIQCFEW R+   EVD
Sbjct: 424 SFKPERFEKEE--EAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVD 483

Query: 490 LSPGIGLTMPKATPLEAMCKPRASIIHLLS 512
           +  G+G T+PKA PL+A+CK R  +  ++S
Sbjct: 484 MKEGVGNTVPKAIPLKAICKARPFLHKIIS 502

The following BLAST results are available for this feature:

BLAST of HG10014175 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141394.1	7.2e-254	85.28	cytochrome P450 81Q32 [Cucumis sativus] >KGN55244.1 hypothetical protein Csa_012...	[more]
XP_008452612.1	7.5e-251	84.81	PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo] >KAA0064425.1 isoflavon...	[more]
XP_038900261.1	4.8e-242	83.85	LOW QUALITY PROTEIN: cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_022940149.1	7.0e-241	82.00	cytochrome P450 81E8-like [Cucurbita moschata]	[more]
XP_023523499.1	1.2e-240	81.80	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10014175 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	3.1e-143	52.07	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	3.0e-130	48.98	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	1.3e-128	47.45	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
P93147	3.7e-128	45.61	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
O65790	5.0e-125	45.31	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

BLAST of HG10014175 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2I1	3.5e-254	85.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1	[more]
A0A5D3D9B5	3.6e-251	84.81	Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3BTN1	3.6e-251	84.81	isoflavone 2'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103493583 PE=3 SV=1	[more]
A0A6J1FJ78	3.4e-241	82.00	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=...	[more]
A0A6J1J4B3	2.4e-239	81.25	isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ...	[more]

BLAST of HG10014175 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	2.0e-129	48.21	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37330.1	1.0e-128	49.60	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37430.1	3.6e-126	45.31	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT4G37360.1	8.8e-125	46.63	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT5G36220.1	2.6e-124	46.08	cytochrome p450 81d1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 439..449 score: 56.31 coord: 66..85 score: 44.21 coord: 90..111 score: 24.89 coord: 406..430 score: 41.8 coord: 365..383 score: 38.26 coord: 302..319 score: 32.6 coord: 322..348 score: 36.58 coord: 449..472 score: 36.62
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 449..460 score: 33.15 coord: 440..449 score: 59.91 coord: 313..330 score: 44.32 coord: 366..377 score: 41.42
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..483 e-value: 1.4E-97 score: 327.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 25..506 e-value: 3.1E-125 score: 420.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..503
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 17..477 e-value: 6.8E-22 score: 75.8
None	No IPR available	PANTHER	PTHR24298:SF683	CYTOCHROME P450 FAMILY PROTEIN	coord: 18..506
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 18..506
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 442..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014175.1	HG10014175.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen