HG10014174 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014174 vs. NCBI nr
Match: XP_038896686.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 909.8 bits (2350), Expect = 1.0e-260
Identity = 453/517 (87.62%), Postives = 480/517 (92.84%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVL 60
           MEPHTITWF  FLISL  LFT ILLSKLRK++H KLPPSPPSLPVIGHLHLL+DP HRVL
Sbjct: 1   MEPHTITWFFPFLISL-FLFTFILLSKLRKINHKKLPPSPPSLPVIGHLHLLRDPLHRVL 60

Query: 61  HDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
           H+L NKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG
Sbjct: 61  HNLSNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEM 180
           FAPYGQHWRNMRRIATTELLSNHRLNTYL+IRV+E+KLW+KNLYK +IC   +FV  VEM
Sbjct: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWIKNLYK-LICREDNFV-GVEM 180

Query: 181 KSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRL 240
           KSKLKELSFNIVMRMVSGKRYFGVEV DVEEALEFRE+ +ELLELSGATNPADFL ILR+
Sbjct: 181 KSKLKELSFNIVMRMVSGKRYFGVEVVDVEEALEFRELTKELLELSGATNPADFLTILRV 240

Query: 241 FDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYY 300
           FDFQGL KRMLKASGRADVFLQKLIDE RERR+SRWPE++  K+MI++LLS Q+SQP YY
Sbjct: 241 FDFQGLNKRMLKASGRADVFLQKLIDEQRERRLSRWPEDKHGKSMIQTLLSLQESQPDYY 300

Query: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDI 360
           SDDIIKGHVLTMLAAGTDTTSGTIEW MSLLLNHPTVMKKAWIEIRD VGENRMVEE D+
Sbjct: 301 SDDIIKGHVLTMLAAGTDTTSGTIEWTMSLLLNHPTVMKKAWIEIRDCVGENRMVEEADV 360

Query: 361 SKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWE 420
           S L+Y+EAIIYETLRLFPAAPLLVPHESSEDCRIEGFE+PKGTML+VNAWAIHRDPKVWE
Sbjct: 361 SNLRYMEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEVPKGTMLLVNAWAIHRDPKVWE 420

Query: 421 DPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVD 480
           DP SFRPERFLNWE    ESYKWIPFGMGRRACPGAALAQRV+GLTLATLI CFEWEKVD
Sbjct: 421 DPNSFRPERFLNWE--GVESYKWIPFGMGRRACPGAALAQRVVGLTLATLIQCFEWEKVD 480

Query: 481 EQQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           EQQIDLSE +GITMPKAK LVAMCKPR SMLHLLS L
Sbjct: 481 EQQIDLSEGSGITMPKAKALVAMCKPRNSMLHLLSCL 512

BLAST of HG10014174 vs. NCBI nr
Match: KAA0064424.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK20163.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 864.0 bits (2231), Expect = 6.6e-247
Identity = 435/523 (83.17%), Postives = 477/523 (91.20%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKV-SHTKLPPSPPSLPVIGHLHLLKDPFHRV 60
           MEPHTITWFS   +SL L+ TLILLSKLRKV +  KLPP+PPSLP+IGHLHLLK+PFHRV
Sbjct: 9   MEPHTITWFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRV 68

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           LH+L NKYGPILSL+IGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAV
Sbjct: 69  LHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 128

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVK-NLYKFIICSSGDFVEAV 180
           GFAPYGQHWRNMRRIATTELLSNHRLN YL+IRVEE+KLWVK NLYK+   S GDFV  V
Sbjct: 129 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKW--GSGGDFV-VV 188

Query: 181 EMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
           +MKSKLKELSFN VMRM+SGKRYFGVEVEDVEEA EFREIM+ELLELSGATN ADFL IL
Sbjct: 189 DMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKIL 248

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRK---TMIRSLLSFQQS 300
           R+FD +G++KRM+KASGRADVFLQKLIDE R++RVSRWPEE++++   +MIR+LLS Q+S
Sbjct: 249 RVFDLEGVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQES 308

Query: 301 QPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMV 360
           QP YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHP  MKKAWIEIRD VGEN+MV
Sbjct: 309 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 368

Query: 361 EEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRD 420
           EEGD+S+LKY+EAIIYETLR+FPAAPLLVPHE SEDC IEGFE+PKGTML+VNAW IHRD
Sbjct: 369 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 428

Query: 421 PKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFE 480
           PKVWEDPTSF+PERFLNWE    E+YKWIPFGMGRRACPG ALAQR MGLTLATLI CFE
Sbjct: 429 PKVWEDPTSFQPERFLNWE--GVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 488

Query: 481 WEKVDEQ-QIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           W+KVDEQ QIDLSE +GITMPKAK L AMCKPRTSMLHLL+ L
Sbjct: 489 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

BLAST of HG10014174 vs. NCBI nr
Match: XP_008453067.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 854.0 bits (2205), Expect = 6.8e-244
Identity = 432/523 (82.60%), Postives = 474/523 (90.63%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKV-SHTKLPPSPPSLPVIGHLHLLKDPFHRV 60
           MEPHTITWFS   +SL L+ TLILLSKLRKV +  KLPP+PPSLP+IGHLHLLK+PFHRV
Sbjct: 6   MEPHTITWFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRV 65

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           LH+L NKYGPILSL+IGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAV
Sbjct: 66  LHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 125

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVK-NLYKFIICSSGDFVEAV 180
           GFAPYGQHWRNMRRIATTELLSNHRLN YL+IRVEE+KLWVK NLYK+   S GDFV  V
Sbjct: 126 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKW--GSGGDFV-VV 185

Query: 181 EMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
           +MKSKLKELSFN VMRM+SGKRYFGVEVEDVEEA EFREIM+ELLELSGATN ADFL IL
Sbjct: 186 DMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKIL 245

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRK---TMIRSLLSFQQS 300
           R+FD +G++KRM+KASGRADVFLQKLIDE R++   RWPEE++++   +MIR+LLS Q+S
Sbjct: 246 RVFDLEGVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQES 305

Query: 301 QPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMV 360
           QP YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHP  MKKAWIEIRD VGEN+MV
Sbjct: 306 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 365

Query: 361 EEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRD 420
           EEGD+S+LKY+EAIIYETLR+FPAAPLLVPHE SEDC IEGFE+PKGTML+VNAW IHRD
Sbjct: 366 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 425

Query: 421 PKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFE 480
           PKVWEDPTSF+PERFLNWE    E+YKWIPFGMGRRACPG ALAQR MGLTLATLI CFE
Sbjct: 426 PKVWEDPTSFQPERFLNWE--GVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 485

Query: 481 WEKVDEQ-QIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           W+KVDEQ QIDLSE +GITMPKAK L AMCKPRTSMLHLL+ L
Sbjct: 486 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

BLAST of HG10014174 vs. NCBI nr
Match: XP_004141393.1 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8649872.1 hypothetical protein Csa_012081 [Cucumis sativus])

HSP 1 Score: 852.0 bits (2200), Expect = 2.6e-243
Identity = 432/523 (82.60%), Postives = 472/523 (90.25%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKV-SHTKLPPSPPSLPVIGHLHLLKDPFHRV 60
           MEPH +T  S   +SL L+ TLILLSKLRK+ +  KLPP+PPSLP+IGHLHLLK PF+RV
Sbjct: 1   MEPHALTCLS---LSLLLISTLILLSKLRKLRNQRKLPPTPPSLPIIGHLHLLKQPFYRV 60

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           LHDL NKYGPILSL+IGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTA+
Sbjct: 61  LHDLSNKYGPILSLTIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAM 120

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKN-LYKFIICSSGDFVEAV 180
           GFAPYGQHWRNMRRIATTELLSNHRLNTYL+IRVEE+KLWVKN LYK+     GDFV  V
Sbjct: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLNIRVEELKLWVKNSLYKW-SSGRGDFV-VV 180

Query: 181 EMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
           +MK KLKELSFN VMRM+SGKRY+GVEVEDVEEALEFREIM+ELLELSGATN ADF  IL
Sbjct: 181 DMKYKLKELSFNTVMRMISGKRYYGVEVEDVEEALEFREIMKELLELSGATNAADFFTIL 240

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRK---TMIRSLLSFQQS 300
           R+FD +G++K+M+KASGRADVFLQKLIDE RE+ VSRWPEE++++   +MIR+LLS Q+S
Sbjct: 241 RVFDIEGVKKKMMKASGRADVFLQKLIDEEREKGVSRWPEEKQKEGKTSMIRTLLSLQES 300

Query: 301 QPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMV 360
           QP YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHP VM+KAWIEIRD +GEN+MV
Sbjct: 301 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMVMEKAWIEIRDCIGENQMV 360

Query: 361 EEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRD 420
           EEGD+SKLKY+EAIIYETLR+FPAAPLLVPHE SEDC IEGFEIPKGTMLMVNAWAIHRD
Sbjct: 361 EEGDVSKLKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEIPKGTMLMVNAWAIHRD 420

Query: 421 PKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFE 480
           PKVWEDPTSFRPERFLNWE    ESYKWIPFGMGRRACPG ALAQR MGLTLATLI CFE
Sbjct: 421 PKVWEDPTSFRPERFLNWE--GVESYKWIPFGMGRRACPGFALAQRSMGLTLATLIQCFE 480

Query: 481 WEKVDE-QQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           WEKVDE +QIDLSE +GITMPKAK L AMCKPR SMLHLL+ L
Sbjct: 481 WEKVDENEQIDLSEGSGITMPKAKALEAMCKPRNSMLHLLAQL 516

BLAST of HG10014174 vs. NCBI nr
Match: XP_023535658.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.6 bits (2139), Expect = 3.1e-236
Identity = 414/518 (79.92%), Postives = 458/518 (88.42%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVL 60
           ME  TITW     +SLSLLFTLIL+ KLRK S  K PPSPPSLP+IGHLHLLK PFHRVL
Sbjct: 20  MEASTITW-----LSLSLLFTLILIHKLRKQSPRKCPPSPPSLPLIGHLHLLKQPFHRVL 79

Query: 61  HDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
            DL NK+GPILSLSIGSRP+VV+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT V 
Sbjct: 80  QDLSNKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVA 139

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEM 180
           FA YG HWRNMRRIATTELLSNHRLNTYL+IRV+E+KLWVKNLY      S DFV  +EM
Sbjct: 140 FASYGHHWRNMRRIATTELLSNHRLNTYLNIRVQELKLWVKNLYG-ATGESSDFVR-LEM 199

Query: 181 KSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRL 240
           +SKL ELSFN VMRMVSGKRYFGV+VEDVEEA EFREIM+ELL+LSGA+NPADFLPILRL
Sbjct: 200 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLSGASNPADFLPILRL 259

Query: 241 FDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEER-RKTMIRSLLSFQQSQPHY 300
           FD+QGLEKRM+KASGRAD+FLQ LID  RERR S WPEE+   KTMI+SLLS Q+SQP Y
Sbjct: 260 FDYQGLEKRMVKASGRADLFLQNLIDCERERRASPWPEEKHGNKTMIQSLLSLQESQPQY 319

Query: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGD 360
           YSDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHPT+M KAW EIR+ VGE+R+VEEGD
Sbjct: 320 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMNKAWSEIRECVGESRLVEEGD 379

Query: 361 ISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVW 420
           +S LKYVEAIIYETLRLFP APLLVPHESSE+CRIEGF+IPKGTML+VNAWAIHRDP++W
Sbjct: 380 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 439

Query: 421 EDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKV 480
           EDPTSFRPER LNWE A  ES+KW+PFG+GRRACPGAALA RV+GLTLATLI CFEW++V
Sbjct: 440 EDPTSFRPERLLNWESA--ESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRV 499

Query: 481 DEQQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
            E+ +DLSE TGIT+PKA PL AMC+ R+SMLHLLS L
Sbjct: 500 GEEPVDLSEGTGITIPKAIPLEAMCRARSSMLHLLSLL 528

BLAST of HG10014174 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.5e-153
Identity = 280/513 (54.58%), Postives = 366/513 (71.35%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDPFHRV 60
           ME  T+ +    ++ LS+   L  +S+ R+     LPPSP  +LPVIGHLHL+    HR 
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRR----NLPPSPGLALPVIGHLHLIGKLLHRS 60

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           L+DL  KYG + SL +G+R V+VVSSP A  ECFTKNDIVFANRP  + GKYI YNYT +
Sbjct: 61  LYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTM 120

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVE 180
             +PYG+HWRN+RR+A  E+ S   LN +LSIR +EVK  + +LY+    SSG     VE
Sbjct: 121 VGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQ----SSGQDFGKVE 180

Query: 181 MKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILR 240
           MKSKL ELSFN+ MRMV+GKRYFG +V D +EA  FR ++ E+ E +GA+NP DF+P LR
Sbjct: 181 MKSKLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLR 240

Query: 241 LFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHY 300
             DF+  EK++ K S   D FLQ+LI E R  +           TMI  LLS Q+SQP Y
Sbjct: 241 WIDFKNYEKKVSKISQEMDAFLQRLIHESRINK--------NNVTMIDHLLSLQESQPEY 300

Query: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGD 360
           Y+D IIKG ++ +L AGTDT++ T+EWAMSLLLNHP  ++KA  EI   VG NR++EE D
Sbjct: 301 YTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQD 360

Query: 361 ISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVW 420
           + KL Y+  II ET RL PAAP+LVPHESS+DC+++G+++PKGT+L+VNAWAIHRDP+ W
Sbjct: 361 LPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFW 420

Query: 421 EDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKV 480
           ++PT F+PER    E    E  K +PFGMGRR+CPG+ LAQRV+GLTL  LI CFEW+++
Sbjct: 421 DEPTLFKPERHGGVE---LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRI 480

Query: 481 DEQQIDLSEATGITMPKAKPLVAMCKPRTSMLH 513
            E +ID++E +G+TMPKA+PL A+CKPR ++LH
Sbjct: 481 GEAKIDMAEGSGLTMPKAQPLEALCKPR-NILH 492

BLAST of HG10014174 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.5e-145
Identity = 275/496 (55.44%), Postives = 335/496 (67.54%), Query Frame = 0

Query: 16  LSLLFTLILLSKLRKVSH--TKLPPSPPSLPVIGHLHLLKDPFHRVLHDLFNKYGPILSL 75
           +SL F +I L      S     LPP P  LP+IG+LH LK P H   H L  KYG I SL
Sbjct: 10  ISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSL 69

Query: 76  SIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRNMRR 135
             GSR VVVVSS T  +ECFTKNDIV ANRP  L+GKYI YN T V  +PYG HWRN+RR
Sbjct: 70  WFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRR 129

Query: 136 IATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKELSFNIVM 195
           I + E+LS+HRLN++L IR +E+   ++ + K    S   F E VE++    E++FN +M
Sbjct: 130 ILSIEILSSHRLNSFLEIRRDEI---MRLIQKLAQKSYNGFTE-VELRPMFSEMTFNTIM 189

Query: 196 RMVSGKRYFG--VEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQGLEKRML 255
           RMVSGKRY+G   +V DVEEA  FR I++E++ L GA N  DFL  LR FDF GLEKR+ 
Sbjct: 190 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLK 249

Query: 256 KASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDIIKGHVLT 315
           K S R D FLQ LIDEHR  +           TMI  LL+ QQSQP YY+D IIKG ++ 
Sbjct: 250 KISKRTDAFLQGLIDEHRFGK-------RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVV 309

Query: 316 MLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKYVEAIIY 375
           ML AGTDT+S TIEWAMS LLNHP +MKKA  E+   +G +R V+E DISKL Y+++I+Y
Sbjct: 310 MLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVY 369

Query: 376 ETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRPERFL 435
           ETLRL  AAPLLVPH SSED  + G+ IP+ T+LMVNAW IHRDP +W DPT F+PERF 
Sbjct: 370 ETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERF- 429

Query: 436 NWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQIDLSEATG 495
              E   E  K + FG+GRRACPG  L+QR  GLTL  LI CFEW+++ E++ID+ EA G
Sbjct: 430 ---EKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKG 489

Query: 496 ITMPKAKPLVAMCKPR 508
           IT  K   L AMCK R
Sbjct: 490 ITAGKKTSLNAMCKVR 490

BLAST of HG10014174 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.9e-144
Identity = 259/495 (52.32%), Postives = 338/495 (68.28%), Query Frame = 0

Query: 13  LISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVLHDLFNKYGPILS 72
           L+SLS + T+ +L K+       LPP PP++P+IG+LH LK P HR    L   YG I S
Sbjct: 7   LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 66

Query: 73  LSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRNMR 132
           L  GSR VVVVSSP+   ECFTKNDI+ ANRPR L+GKYI YNYT +G A YG HWRN+R
Sbjct: 67  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126

Query: 133 RIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKELSFNIV 192
           RI T ++LSN+RLN++L +R +E    ++ L K ++     F + VE++ +L E++FN +
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTK-VELRPRLTEMTFNAM 186

Query: 193 MRMVSGKRYFG--VEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQGLEKRM 252
           MRM+SGKRY+G   +V DVEEA +FREI+ E++ L GA N  DFLP+LR+ D   LEKR 
Sbjct: 187 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 246

Query: 253 LKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDIIKGHVL 312
            + + R++ FL+ LI+EHR   +          TMI  LL   +SQP YYSD +IKG + 
Sbjct: 247 KRIAKRSNAFLEGLIEEHRRGNI-----HSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 306

Query: 313 TMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKYVEAII 372
            ML AGTDT++ TIEW MS LLNHP V+KKA  E+   +G+N++V+E D+SKL Y++ II
Sbjct: 307 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 366

Query: 373 YETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRPERF 432
            ETLRL P APLL+PH SSEDC I  F +PK T+++ N W IHRDPK W D  SF+PERF
Sbjct: 367 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 426

Query: 433 LNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQIDLSEAT 492
               E   E  K + FG+GRRACPG +LAQR +G T+  LI CFEWE+  E+++D+ E  
Sbjct: 427 ----EKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGK 486

Query: 493 GITMPKAKPLVAMCK 506
           GITMP   PL AMCK
Sbjct: 487 GITMPMKIPLRAMCK 491

BLAST of HG10014174 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 506.9 bits (1304), Expect = 2.7e-142
Identity = 254/509 (49.90%), Postives = 354/509 (69.55%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVL 60
           ME  ++  +S F ++L  +F +++ ++  K     LPP PPSLP+IG+LH LK P HR  
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFK----NLPPGPPSLPIIGNLHHLKRPLHRTF 60

Query: 61  HDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
             L  KYG + SL  GSR VVVVSS +  ++CFTKND+V ANRPR LSGKYI YNYT +G
Sbjct: 61  KGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLG 120

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEM 180
              YG+HWRN+RRI   ++LSNHR+N++  IR +E +  +  L      SS +F E +E+
Sbjct: 121 STSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLAD---DSSTNFAE-MEL 180

Query: 181 KSKLKELSFNIVMRMVSGKRYFG--VEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
            S+L +++FN +MRM+SGKRY+G   +  D++EA +FR+++ ELL+LSGA N  DF+P+L
Sbjct: 181 SSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLL 240

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPH 300
           R  DF+ LEKR+   SG+ D FL+ LI+EHR ++       ER  TMI  LL+ Q SQP 
Sbjct: 241 RFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKK-------ERANTMIDHLLNLQDSQPE 300

Query: 301 YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEG 360
           YY+D IIKG  L ML AGTD+++ T+EW+MS LLNHP V+KK   E+   VG++R+V+E 
Sbjct: 301 YYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDES 360

Query: 361 DISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKV 420
           D+ KL Y++ +I ETLRL+  APLL+PH +S++C I G+++P+ T++++NAWAIHRDP++
Sbjct: 361 DLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPEL 420

Query: 421 WEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEK 480
           W + T+F+PERF    E   E  K I FGMGRRACPG  LA R + +TLA LI CF+W+ 
Sbjct: 421 WTEATTFKPERF----EKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKL 480

Query: 481 VDEQQIDLSEATGITMPKAKPLVAMCKPR 508
           ++  +IDL+E  G T+ K  PL AMCK R
Sbjct: 481 INGDKIDLAERDGFTLTKLVPLKAMCKSR 490

BLAST of HG10014174 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.3e-138
Identity = 246/497 (49.50%), Postives = 347/497 (69.82%), Query Frame = 0

Query: 13  LISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVLHDLFNKYGPILS 72
           L  LS+ F + LL + RK  +  LPP P SLP+IG+LH LK P +R    L  KYG ++S
Sbjct: 11  LFYLSIFFIIRLLFQSRKFKN--LPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVIS 70

Query: 73  LSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWRNMR 132
           L  GSR VVVVSS +  +ECFTKND+V ANRPR LSGKYI YNYT +G   YG+HWRN+R
Sbjct: 71  LWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 130

Query: 133 RIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKELSFNIV 192
           RI + ++LSNHR+N +  IR +E +  +K L +    SS  F E VE+  +  +++FN +
Sbjct: 131 RITSLDVLSNHRINNFAPIRRDETQRLIKKLAE---DSSTKFAE-VELTFRFFDMTFNNI 190

Query: 193 MRMVSGKRYFG--VEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQGLEKRM 252
           MRM+SGKRY+G   ++ +V+EA +FR+++ ELL+LSGA N  DF+P+L+  DF+ LEKR+
Sbjct: 191 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 250

Query: 253 LKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDIIKGHVL 312
            +   + DVFL  L+ E R ++       ER  TMI  LL+ Q+SQP YY+D IIKG  L
Sbjct: 251 KRIGEKNDVFLSGLLQEQRSKK-------ERTNTMIDHLLNMQESQPEYYTDTIIKGLCL 310

Query: 313 TMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKYVEAII 372
            ML AGTD+++ T+EW MS +LN+P V+KK   E+   VG++R+V+E D+ KL Y+  +I
Sbjct: 311 AMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVI 370

Query: 373 YETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRPERF 432
           YETLRL+  APLL+PH ++++C + G+++P+ T++++NAWAIHRDP+ W + T+F+PERF
Sbjct: 371 YETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERF 430

Query: 433 LNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQIDLSEAT 492
               +   E  K I FGMGRRACPG  LA R + +TLA L+ CF+W+++++++ID+SE  
Sbjct: 431 ----DKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERD 490

Query: 493 GITMPKAKPLVAMCKPR 508
           G TM K  PL AMCK R
Sbjct: 491 GFTMTKLLPLKAMCKTR 490

BLAST of HG10014174 vs. ExPASy TrEMBL
Match: A0A5A7VB12 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002950 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.2e-247
Identity = 435/523 (83.17%), Postives = 477/523 (91.20%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKV-SHTKLPPSPPSLPVIGHLHLLKDPFHRV 60
           MEPHTITWFS   +SL L+ TLILLSKLRKV +  KLPP+PPSLP+IGHLHLLK+PFHRV
Sbjct: 9   MEPHTITWFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRV 68

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           LH+L NKYGPILSL+IGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAV
Sbjct: 69  LHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 128

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVK-NLYKFIICSSGDFVEAV 180
           GFAPYGQHWRNMRRIATTELLSNHRLN YL+IRVEE+KLWVK NLYK+   S GDFV  V
Sbjct: 129 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKW--GSGGDFV-VV 188

Query: 181 EMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
           +MKSKLKELSFN VMRM+SGKRYFGVEVEDVEEA EFREIM+ELLELSGATN ADFL IL
Sbjct: 189 DMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKIL 248

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRK---TMIRSLLSFQQS 300
           R+FD +G++KRM+KASGRADVFLQKLIDE R++RVSRWPEE++++   +MIR+LLS Q+S
Sbjct: 249 RVFDLEGVKKRMMKASGRADVFLQKLIDEERKKRVSRWPEEKQKQAKTSMIRTLLSLQES 308

Query: 301 QPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMV 360
           QP YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHP  MKKAWIEIRD VGEN+MV
Sbjct: 309 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 368

Query: 361 EEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRD 420
           EEGD+S+LKY+EAIIYETLR+FPAAPLLVPHE SEDC IEGFE+PKGTML+VNAW IHRD
Sbjct: 369 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 428

Query: 421 PKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFE 480
           PKVWEDPTSF+PERFLNWE    E+YKWIPFGMGRRACPG ALAQR MGLTLATLI CFE
Sbjct: 429 PKVWEDPTSFQPERFLNWE--GVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 488

Query: 481 WEKVDEQ-QIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           W+KVDEQ QIDLSE +GITMPKAK L AMCKPRTSMLHLL+ L
Sbjct: 489 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 523

BLAST of HG10014174 vs. ExPASy TrEMBL
Match: A0A1S3BWG9 (LOW QUALITY PROTEIN: cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493891 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.3e-244
Identity = 432/523 (82.60%), Postives = 474/523 (90.63%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKV-SHTKLPPSPPSLPVIGHLHLLKDPFHRV 60
           MEPHTITWFS   +SL L+ TLILLSKLRKV +  KLPP+PPSLP+IGHLHLLK+PFHRV
Sbjct: 6   MEPHTITWFS---LSLLLITTLILLSKLRKVRNQKKLPPTPPSLPIIGHLHLLKEPFHRV 65

Query: 61  LHDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAV 120
           LH+L NKYGPILSL+IGSRPV+VVSSPTAVRECFTKNDIVFANRPRL+SGKYINYNYTAV
Sbjct: 66  LHNLSNKYGPILSLTIGSRPVLVVSSPTAVRECFTKNDIVFANRPRLISGKYINYNYTAV 125

Query: 121 GFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVK-NLYKFIICSSGDFVEAV 180
           GFAPYGQHWRNMRRIATTELLSNHRLN YL+IRVEE+KLWVK NLYK+   S GDFV  V
Sbjct: 126 GFAPYGQHWRNMRRIATTELLSNHRLNMYLNIRVEELKLWVKNNLYKW--GSGGDFV-VV 185

Query: 181 EMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPIL 240
           +MKSKLKELSFN VMRM+SGKRYFGVEVEDVEEA EFREIM+ELLELSGATN ADFL IL
Sbjct: 186 DMKSKLKELSFNTVMRMISGKRYFGVEVEDVEEAFEFREIMKELLELSGATNAADFLKIL 245

Query: 241 RLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRK---TMIRSLLSFQQS 300
           R+FD +G++KRM+KASGRADVFLQKLIDE R++   RWPEE++++   +MIR+LLS Q+S
Sbjct: 246 RVFDLEGVKKRMMKASGRADVFLQKLIDEERKKEF-RWPEEKQKQAKTSMIRTLLSLQES 305

Query: 301 QPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMV 360
           QP YYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHP  MKKAWIEIRD VGEN+MV
Sbjct: 306 QPQYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPMTMKKAWIEIRDCVGENQMV 365

Query: 361 EEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRD 420
           EEGD+S+LKY+EAIIYETLR+FPAAPLLVPHE SEDC IEGFE+PKGTML+VNAW IHRD
Sbjct: 366 EEGDVSELKYLEAIIYETLRMFPAAPLLVPHECSEDCSIEGFEVPKGTMLLVNAWGIHRD 425

Query: 421 PKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFE 480
           PKVWEDPTSF+PERFLNWE    E+YKWIPFGMGRRACPG ALAQR MGLTLATLI CFE
Sbjct: 426 PKVWEDPTSFQPERFLNWE--GVETYKWIPFGMGRRACPGVALAQRSMGLTLATLIQCFE 485

Query: 481 WEKVDEQ-QIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           W+KVDEQ QIDLSE +GITMPKAK L AMCKPRTSMLHLL+ L
Sbjct: 486 WKKVDEQEQIDLSEGSGITMPKAKALEAMCKPRTSMLHLLAQL 519

BLAST of HG10014174 vs. ExPASy TrEMBL
Match: A0A6J1F8L9 (isoflavone 3'-hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443310 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.3e-236
Identity = 414/518 (79.92%), Postives = 458/518 (88.42%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVL 60
           ME +TITW     +SLSLLFTL L+ KLRK S  + PPSPPSLP+IGHLHLLK PFHRVL
Sbjct: 1   MEANTITW-----LSLSLLFTLSLIYKLRKQSPRRCPPSPPSLPLIGHLHLLKQPFHRVL 60

Query: 61  HDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
            DL +K+GPILSLSIGSRP+VV+SSPTAVRECFTKNDI FANRPRLLSGKY+NYNYT V 
Sbjct: 61  QDLSDKHGPILSLSIGSRPMVVISSPTAVRECFTKNDIAFANRPRLLSGKYMNYNYTGVA 120

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEM 180
            A YG HWRNMRRIATTELLSNHRLN YL+IRV+E+KLWVKNLY      S DFV  +EM
Sbjct: 121 VASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKLWVKNLYG-ETGESSDFVR-LEM 180

Query: 181 KSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRL 240
           +SKL ELSFN VMRMVSGKRYFGV+VEDVEEA EFREIM+ELL LSGA+NPADFLPILRL
Sbjct: 181 QSKLNELSFNTVMRMVSGKRYFGVDVEDVEEAREFREIMKELLHLSGASNPADFLPILRL 240

Query: 241 FDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEER-RKTMIRSLLSFQQSQPHY 300
           FD+QGLEKRM+KASGRAD+FLQ LID  RERR SRWPEE+   KTMI+SLLSFQ+SQP Y
Sbjct: 241 FDYQGLEKRMVKASGRADLFLQNLIDCERERRASRWPEEKHGNKTMIQSLLSFQESQPQY 300

Query: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGD 360
           YSDDIIKGHVLTMLAAGTDTT+GTIEWAMSLLLNHPT+M KAW EIR+ VGE+R+VEEGD
Sbjct: 301 YSDDIIKGHVLTMLAAGTDTTTGTIEWAMSLLLNHPTIMDKAWSEIRECVGESRLVEEGD 360

Query: 361 ISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVW 420
           +S LKYVEAIIYETLRLFP APLLVPHESSE+CRIEGF+IPKGTML+VNAWAIHRDP++W
Sbjct: 361 VSSLKYVEAIIYETLRLFPVAPLLVPHESSEECRIEGFDIPKGTMLLVNAWAIHRDPQLW 420

Query: 421 EDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKV 480
           EDPTSFRPERFLNWE  A ES+KW+PFG+GRRACPG ALA RV+GLTLATLI CFEW++V
Sbjct: 421 EDPTSFRPERFLNWE--AAESHKWVPFGVGRRACPGVALAHRVVGLTLATLIQCFEWQRV 480

Query: 481 DEQQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
            E+ IDLSE TGITMPKA PLVAMC+ R+SMLHLLS L
Sbjct: 481 GEEPIDLSEGTGITMPKAIPLVAMCRARSSMLHLLSPL 509

BLAST of HG10014174 vs. ExPASy TrEMBL
Match: A0A6J1IL26 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477203 PE=3 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.5e-228
Identity = 404/518 (77.99%), Postives = 453/518 (87.45%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVL 60
           ME +TIT      +SLSLL TLIL+ KLRK S    PPSPPSLP+IGHLHLLK PFHRVL
Sbjct: 12  MEANTIT-----CLSLSLLLTLILIYKLRKQSPRNRPPSPPSLPLIGHLHLLKQPFHRVL 71

Query: 61  HDLFNKYGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVG 120
            DL NK+GPILSLSIGSRP+VV+SSPTAVRECFTKNDI  ANRPRLL+GKY+NYNYT V 
Sbjct: 72  QDLSNKHGPILSLSIGSRPLVVISSPTAVRECFTKNDIALANRPRLLAGKYMNYNYTGVA 131

Query: 121 FAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEM 180
           FA YG HWRNMRRIATTELLSNHRLN YL+IRV+E+KL VKNLY      S DFV  +EM
Sbjct: 132 FASYGHHWRNMRRIATTELLSNHRLNAYLNIRVQELKL-VKNLYG-ATGESSDFVR-LEM 191

Query: 181 KSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRL 240
           +SKL ELSFNIVMRMVSGKRYFGV+VEDVEEA EFREIM+ELL+L GA+NPADFLPILRL
Sbjct: 192 QSKLNELSFNIVMRMVSGKRYFGVDVEDVEEAREFREIMKELLQLGGASNPADFLPILRL 251

Query: 241 FDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEER-RKTMIRSLLSFQQSQPHY 300
           FD+QGLEKRM++ASGRAD FLQ LID  R+RR SRWPEE+R  KTMI+SLLS Q+SQP +
Sbjct: 252 FDYQGLEKRMVQASGRADSFLQNLIDCERDRRASRWPEEKRGNKTMIQSLLSLQESQPQH 311

Query: 301 YSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGD 360
           YSDDIIKGHVLTMLAAGTDTT+GT+EWAMSLLLNHP +M KAW EIR+ VGE+R+VEEGD
Sbjct: 312 YSDDIIKGHVLTMLAAGTDTTTGTLEWAMSLLLNHPAIMNKAWSEIRECVGESRLVEEGD 371

Query: 361 ISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVW 420
           +S LKY+ AIIYETLRLFPAAPLLVPHESSE+CR+EGF+IPKGTML+VNAWAIHRDP+VW
Sbjct: 372 VSSLKYLVAIIYETLRLFPAAPLLVPHESSEECRVEGFDIPKGTMLLVNAWAIHRDPRVW 431

Query: 421 EDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKV 480
           EDPTSFRPERFLNWE A  ES+KW+PFG+GRRACPGAALA RV+GLTLATLI CFEW++V
Sbjct: 432 EDPTSFRPERFLNWETA--ESHKWVPFGVGRRACPGAALAHRVVGLTLATLIQCFEWQRV 491

Query: 481 DEQQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
            E+ IDLS+ TGIT PKA PL AMC+ R+SMLHLLS L
Sbjct: 492 GEEPIDLSQGTGITTPKAIPLEAMCRARSSMLHLLSPL 519

BLAST of HG10014174 vs. ExPASy TrEMBL
Match: A0A6J1C9L7 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 1.1e-183
Identity = 325/512 (63.48%), Postives = 401/512 (78.32%), Query Frame = 0

Query: 9   FSGFLISLSLLFTLILL--SKLRKVSHTKLPPSPPSLPVIGHLHLLKDPFHRVLHDLFNK 68
           FS FLI L+LLF LI +   KL+KVS   LPP PPSLPVIGHLHLL++PFH++L +L  K
Sbjct: 7   FSRFLIPLALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSKK 66

Query: 69  YGPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQ 128
           YGPILSLS+G R VVVVSSP+A +ECFTKNDIVFANRPR++SGK +NYNYT+VG   YGQ
Sbjct: 67  YGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSVGATAYGQ 126

Query: 129 HWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKE 188
           HWR MRRIATT+LLS HRLN+++++R +EVKLW+KNLY+    ++G     VE+++KL E
Sbjct: 127 HWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQ----TTGQNNVQVEIRTKLTE 186

Query: 189 LSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQGL 248
           LSFNI +RM++GKRYFGV+VED EE  + +++M+E+  LSGA+ PADFL +L+L D+QG 
Sbjct: 187 LSFNIALRMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLIDYQGF 246

Query: 249 EKRMLKASGRADVFLQKLIDEHRERRVS-RWPEEERRKTMIRSLLSFQQSQPHYYSDDII 308
            KR+ K    AD F Q LID  RER+ S  +  EE RK MI ++LS Q S P YY D II
Sbjct: 247 RKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYKDHII 306

Query: 309 KGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKY 368
           KG +LT+LAAGTDTT+GTIEW MSLLLNH T+MKK W EI + VGE R+VEE D++ L +
Sbjct: 307 KGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLTNLPF 366

Query: 369 VEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSF 428
           ++AII ET RLFPA P+LVP ESSEDC I G++IPKGTML+VN WAIHRDPKVW+DPTSF
Sbjct: 367 LQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQDPTSF 426

Query: 429 RPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQID 488
           +PERF N E    E+ K +PFGMGRRACPG+ LA RV+GL LATLI CFEW+K+   +ID
Sbjct: 427 QPERFENGE--VVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNEID 486

Query: 489 LSEATGITMPKAKPLVAMCKPRTSMLHLLSHL 518
           L +  G+TMPKA PL AMCKPR  M++LLS L
Sbjct: 487 LFQGNGLTMPKAHPLEAMCKPRAPMINLLSQL 512

BLAST of HG10014174 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 488.8 bits (1257), Expect = 5.4e-138
Identity = 256/518 (49.42%), Postives = 359/518 (69.31%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDPFHRV 60
           ME  T+  FS   + LSL++   L+ KL++     LPPSP  SLPVIGHL LLK P HR 
Sbjct: 1   METKTLI-FSILFVVLSLIY---LIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRT 60

Query: 61  LHDLFNKY--GPILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYT 120
              L       PI SL +G+R V V SS +   ECFTKND+V ANRP  +  K++ Y+YT
Sbjct: 61  FLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYT 120

Query: 121 AVGFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEA 180
            +  A YG HWRN+RRI + E+ SNHRLN++LSIR +E++  V  L +     S +FV+ 
Sbjct: 121 TMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNF---SQEFVK- 180

Query: 181 VEMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPI 240
           V+MKS L +L+FN ++RMV+GKRY+G  VED  EA   R+++ +++  +GA N  D+LP+
Sbjct: 181 VDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPV 240

Query: 241 LRLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQP 300
           LRL      E R+ K +GR D FLQ L+DE RE +       E+  TMI  LL+ Q+SQP
Sbjct: 241 LRL--VSDYETRVKKLAGRLDEFLQGLVDEKREAK-------EKGNTMIDHLLTLQESQP 300

Query: 301 HYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEE 360
            Y++D IIKG++L ++ AGTDT++ T+EWA+S +LNHP V+ KA  EI   +G +R+++E
Sbjct: 301 DYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDE 360

Query: 361 GDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPK 420
            DIS L Y++ I+ ETLRL+PAAP+L+PH +SEDC++ G+++P+GT+L+ N WAIHRDP+
Sbjct: 361 SDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQ 420

Query: 421 VWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWE 480
           +W+DP SF+PERF    E   E+ K +PFG+GRRACPG+ LA R++ LTL +LI C EWE
Sbjct: 421 LWDDPMSFKPERF----EKEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWE 480

Query: 481 KVDEQQIDLSEATGITMPKAKPLVAMCKPRTSMLHLLS 516
           K+ E ++D+SE  G+TMPKAKPL AMC+ R S++ + +
Sbjct: 481 KIGE-EVDMSEGKGVTMPKAKPLEAMCRARPSVVKIFN 494

BLAST of HG10014174 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 479.9 bits (1234), Expect = 2.5e-135
Identity = 258/515 (50.10%), Postives = 351/515 (68.16%), Query Frame = 0

Query: 1   MEPHTITWFSGFLISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDPFHRV 60
           ME  T+  F+   ISLSL F   ++ ++++     LPPSP  +LPVIGHL LLK P HRV
Sbjct: 1   MEGQTLI-FTFLFISLSLTF---IIGRIKR--RPNLPPSPSWALPVIGHLRLLKPPLHRV 60

Query: 61  LHDLFNKYG--PILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYT 120
              +    G  PI+SL +G+R V VVSS +   ECFTKND+V ANR   L+ K+I+Y  T
Sbjct: 61  FLSVSESLGDAPIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCT 120

Query: 121 AVGFAPYGQHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIIC----SSGD 180
            V  A YG HWRN+RRI   E+ S HRLN++ SIR +E+       ++ I C    SS +
Sbjct: 121 TVVTASYGDHWRNLRRIGAVEIFSAHRLNSFSSIRRDEI-------HRLIACLSRNSSLE 180

Query: 181 FVEAVEMKSKLKELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPAD 240
           F + VEMKS    L+FN ++RM++GK Y+G   ED  EA   RE++ E +   GA N AD
Sbjct: 181 FTK-VEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTAD 240

Query: 241 FLPILRLFDFQGLEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQ 300
           +LPIL      G EKR+ K + R D FLQ L+DE RE +      E+R+ TM+  LL  Q
Sbjct: 241 YLPILTW--ITGSEKRIKKIASRLDEFLQGLVDERREGK------EKRQNTMVDHLLCLQ 300

Query: 301 QSQPHYYSDDIIKGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENR 360
           ++QP YY+D+IIKG +L+++ AGTDT++ T+EW +S LLNHP ++ KA  EI + VG NR
Sbjct: 301 ETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNR 360

Query: 361 MVEEGDISKLKYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIH 420
           +VEE D+S L Y++ I+ E+LRL+PA+PLLVPH +SEDC++ G+ +P+GTML+ NAWAIH
Sbjct: 361 LVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIH 420

Query: 421 RDPKVWEDPTSFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHC 480
           RDPK+W+DPTSF+PERF    E   E+ K + FG+GRRACPG+ LAQR+  LT+ +LI C
Sbjct: 421 RDPKIWDDPTSFKPERF----EKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQC 480

Query: 481 FEWEKVDEQQIDLSE-ATGITMPKAKPLVAMCKPR 508
           FEWE++ E+++D++E   G+ MPKA PLVAMCK R
Sbjct: 481 FEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKAR 489

BLAST of HG10014174 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 475.3 bits (1222), Expect = 6.1e-134
Identity = 251/499 (50.30%), Postives = 333/499 (66.73%), Query Frame = 0

Query: 13  LISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDPFHRVLHDLFNKYG--P 72
           ++S   LFTL +    R      LPPSP  SLPVIGHLHLLK P HR    L    G  P
Sbjct: 8   ILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAP 67

Query: 73  ILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWR 132
           +  L +G+R V V+SS +   ECFTKND+V ANRP+    K++ YN T +  A YG HWR
Sbjct: 68  VFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWR 127

Query: 133 NMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKELSF 192
           N+RRIA  E+ S HRLN++L IR +E++  + +L +    S   FVE VEMK+ L  L+ 
Sbjct: 128 NLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSR---DSLHGFVE-VEMKTLLTNLAS 187

Query: 193 NIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQGLEKR 252
           N  +RM++GKRYFG   ED ++A   + ++ E +  +GA NP D+L ILR       EKR
Sbjct: 188 NTTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--VSSYEKR 247

Query: 253 MLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDIIKGHV 312
           +     R D FLQKL+DE R        E+E+ +TMI  LL+ Q  QP YY+D IIKG +
Sbjct: 248 IKNLGNRFDTFLQKLVDEKR-------AEKEKGETMIDHLLALQDIQPDYYTDVIIKGII 307

Query: 313 LTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKYVEAI 372
           LT++ AGTDT+S T+EWAMS LLNHP ++KKA +EI + VG +R+V+E DI  L Y+++I
Sbjct: 308 LTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSI 367

Query: 373 IYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSFRPER 432
           + ETLR++PA PLL+PH SSEDC++ G++IP GTM++ NAWA+HRDP+VWEDP  F+PER
Sbjct: 368 VLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER 427

Query: 433 FLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQIDLSEA 492
           F    E   E+ K I FGMGRRACPGA LA R++   L +L+ CFEWE+V E  +D++E 
Sbjct: 428 F----EKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTED 486

Query: 493 TGITMPKAKPLVAMCKPRT 509
            G T+PKA PL AMCK R+
Sbjct: 488 KGATLPKAIPLRAMCKARS 486

BLAST of HG10014174 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 468.0 bits (1203), Expect = 9.8e-132
Identity = 243/502 (48.41%), Postives = 345/502 (68.73%), Query Frame = 0

Query: 9   FSGFLISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDPFHRVLHDLFNKY 68
           F+   I LSL+F   L+ ++++     LPPSP  +LPVIGHL LLK P HRV   +    
Sbjct: 8   FTFCFIVLSLIF---LIGRIKR--KLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSL 67

Query: 69  G--PILSLSIGSRPVVVVSSPTAVRECFTKNDIVFANRPRLLSGKYINYNYTAVGFAPYG 128
           G  PI+SL +G+R + VVSS +   ECFTKND++ ANR   +S K+I+Y  + V  A Y 
Sbjct: 68  GDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYS 127

Query: 129 QHWRNMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLK 188
           +HWRN+RRI   E+ S HRLN++ SIR +E++  +  L +    SS  F + VEMKS   
Sbjct: 128 EHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLR---NSSYGFTK-VEMKSMFS 187

Query: 189 ELSFNIVMRMVSGKRYFGVEVEDVEEALEFREIMRELLELSGATNPADFLPILRLFDFQG 248
           +L+FN ++RM++GK Y+G   ED  EA   R ++ E +  SG  N AD++PIL    +  
Sbjct: 188 DLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS- 247

Query: 249 LEKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDII 308
            E R+ K +GR D FLQ L+DE RE +      E++  TM+  LL  Q++QP YY D II
Sbjct: 248 -ETRIKKLAGRLDEFLQGLVDEKREGK------EKKENTMVDHLLCLQETQPEYYMDRII 307

Query: 309 KGHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEIRDSVGENRMVEEGDISKLKY 368
           KG +L+++A GTDTT+ T+EWA+S LLN+P V+ KA  EI   +G +R++EE DI  L Y
Sbjct: 308 KGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPY 367

Query: 369 VEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPTSF 428
           ++ I+ ETLRL+PAAP+L+PH +S+DC++ G+++P+GTML+ NAWAIHRDP +W+DPTSF
Sbjct: 368 LQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSF 427

Query: 429 RPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQID 488
           +PERF    E   E+ K +PFG+GRRACPG+ LAQR++ L+L +LI CFEWE++ E+++D
Sbjct: 428 KPERF----EKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVD 487

Query: 489 LSEATGITMPKAKPLVAMCKPR 508
           ++E  G+TMPKA+PL AMC+ R
Sbjct: 488 MTEGPGLTMPKARPLEAMCRAR 488

BLAST of HG10014174 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.7e-131
Identity = 243/511 (47.55%), Postives = 338/511 (66.14%), Query Frame = 0

Query: 13  LISLSLLFTLILLSKLRKVSHTKLPPSPP-SLPVIGHLHLLKDP-FHRVLHDLFNKYGPI 72
           L+++ ++  ++L++K  K     LPPSP    P+IGHLHLLK P  HR L  L +  GP+
Sbjct: 11  LVAIFIVKIVLLVTKPNK----NLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPV 70

Query: 73  LSLSIGSRPVVVVSSPTAVRECF-TKNDIVFANRPRLLSGKYINYNYTAVGFAPYGQHWR 132
            SL +GSR  V++SSPTA  ECF TKNDIV ANRPR + GKY+ Y+YT++  APYG HWR
Sbjct: 71  FSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWR 130

Query: 133 NMRRIATTELLSNHRLNTYLSIRVEEVKLWVKNLYKFIICSSGDFVEAVEMKSKLKELSF 192
           N+RRI   E+ S +RLN    IR +EVK+ ++ L+      S +    VE++  L  L+ 
Sbjct: 131 NLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDL----SVERPAKVELRQLLTGLTL 190

Query: 193 NIVMRMVSGKRYFGVEVEDVEEA---LEFREIMRELLELSGATNPADFLPILRLFDFQGL 252
           N++MRM++GKR+F  E +D  +A   LEFRE++ E+LELS A NPADFLP LR FD++GL
Sbjct: 191 NVIMRMMTGKRFF--EEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGL 250

Query: 253 EKRMLKASGRADVFLQKLIDEHRERRVSRWPEEERRKTMIRSLLSFQQSQPHYYSDDIIK 312
            KR  +   R D  LQ  +DEHR  +       E + TMI  LL  Q+ +PH YSD  IK
Sbjct: 251 VKRAKRIGERMDSLLQGFLDEHRANK----DRLEFKNTMIAHLLDSQEKEPHNYSDQTIK 310

Query: 313 GHVLTMLAAGTDTTSGTIEWAMSLLLNHPTVMKKAWIEI---RDSVGENRMVEEGDISKL 372
           G +L M+  GTDT++ T+EWAMS LLNHP +++     I    ++    R+++E D+  +
Sbjct: 311 GLILMMVVGGTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNM 370

Query: 373 KYVEAIIYETLRLFPAAPLLVPHESSEDCRIEGFEIPKGTMLMVNAWAIHRDPKVWEDPT 432
            Y++ ++ ETLRL+P APL+VPH  S DC I GF +P+ T+++VN WAIHRDP VW+DPT
Sbjct: 371 NYLKNVVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPT 430

Query: 433 SFRPERFLNWEEAAPESYKWIPFGMGRRACPGAALAQRVMGLTLATLIHCFEWEKVDEQQ 492
           SF+PERF   ++    + K +PFG+GRRACPG +LA RV+GL L ++I CFEWE     Q
Sbjct: 431 SFKPERFEGSDQFGHYNGKMMPFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQ 490

Query: 493 IDLSEATGITMPKAKPLVAMCKPRTSMLHLL 515
           +D++E  G+++PKA+PLV  C+ R     LL
Sbjct: 491 VDMTEGPGLSLPKAEPLVVTCRTREMASELL 507