HG10014164 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014164
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	Chr02: 8124537 .. 8125833 (+)
RNA-Seq Expression	HG10014164
Synteny	HG10014164

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTCCATCGCCAAGATGATGCCAAAAAACTTCTTACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTAAGCTTTCCCCCTTCCCCTTTCCTATTTTCTTCTTTCTCTTCATGCATCATTCATTTATTACTTTTGTTTTTTTTTTTTTTTTTTATATATATATATATAACCAAATCAACGATCTCCTCTGCATTTTCAAGGTTAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGTACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTCTCCGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCGTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGTTTAATTATATTTCCATTCTGATTCCTTTTTTTTTTCTTTTTTTTTCTTTTTCTGATTTAATCAATTCCACTTTTTCTGACAATTTGGATCGTCTCAGGGTGTGGAACTTGTGAGCAATGACTATTTCATCACCGAAGAAAACGTCGGACTGCTAGAATTAGCCAAAGAAGCCAACAGTATATGGGTACGCCCGCGATAGAATCATTCGTTACAAATAACCGTCCGAAATAGACACACTTTTAACAATTTTCTTTTTGGGTTTTTGATTTGTTATAACAGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCACCAGAGACGGTGGGGTGCGTGGTGGTGGATAGCGAAGGAAGGTGCGCCGCCGCTACATCCACCGGTGGATTGATAAACAAAAAGATGGGACGCATTGGAGACTCGCCGTTGATCGGATCAGGGACGTACGCGTGTGATGTATGTGGGGTATCGTGCACGGGAGAAGGGGAGGCGATCATTAGAGGGACTTTGGCTAGAGAAGTGGCGGCAGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTTTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAA

mRNA sequence

ATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTCCATCGCCAAGATGATGCCAAAAAACTTCTTACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTTAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGTACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTCTCCGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCGTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTGGAACTTGTGAGCAATGACTATTTCATCACCGAAGAAAACGTCGGACTGCTAGAATTAGCCAAAGAAGCCAACAGTATATGGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCACCAGAGACGGTGGGGTGCGTGGTGGTGGATAGCGAAGGAAGGTGCGCCGCCGCTACATCCACCGGTGGATTGATAAACAAAAAGATGGGACGCATTGGAGACTCGCCGTTGATCGGATCAGGGACGTACGCGTGTGATGTATGTGGGGTATCGTGCACGGGAGAAGGGGAGGCGATCATTAGAGGGACTTTGGCTAGAGAAGTGGCGGCAGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTTTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAA

Coding sequence (CDS)

ATGGGAGGTTGGGCCATAGCGGTTCATGGTGGGGCTGGCGTGGATCCTAATCTCCCACTCCATCGCCAAGATGATGCCAAAAAACTTCTTACTCGCTGCCTTGATCTTGGCATCCATGCTCTTCGTTCAAATCATTCTGCCATTGATGTCGTTGAACTCGTCGTTAGAGAATTGGAAACCGATCCCCTGTTCAATTCGGGGCGTGGATCTGCCCTCACAGAGGAGGGTACCGTAGAGATGGAGGCCAGCATCATGGACGGCCCAAAACGGAGATGCGGGGCGGTCTCCGGACTCACCACCGTCAAAAACCCCGTCTCCCTCGCCCGTCTTGTCATGGATAAATCTCCCCATTCGTATCTGGCCTTCTCTGGCGCTGAGAAATTCGCTAGGCAACAGGGTGTGGAACTTGTGAGCAATGACTATTTCATCACCGAAGAAAACGTCGGACTGCTAGAATTAGCCAAAGAAGCCAACAGTATATGGTTGGATCATAGGGTCCCAAGCGAGACGTGTAGCGCGAGCGTGGAGACACCATTGCAAATGAACGGCCTCCCAATCAGCGTGTACGCACCAGAGACGGTGGGGTGCGTGGTGGTGGATAGCGAAGGAAGGTGCGCCGCCGCTACATCCACCGGTGGATTGATAAACAAAAAGATGGGACGCATTGGAGACTCGCCGTTGATCGGATCAGGGACGTACGCGTGTGATGTATGTGGGGTATCGTGCACGGGAGAAGGGGAGGCGATCATTAGAGGGACTTTGGCTAGAGAAGTGGCGGCAGTGATGGAATACAAAGGGTTGGGGCTTCAAGAGGCAGTGAATTACGTGATAGAGGAGAGATTGGACGAAGGCAAGGCGGGATTGATTGCAGTTTCTAGCAATGGGGAAGTGGCGTGTGGGTTCAACACAACCGGGATGTTCAGGGGATGCGCCACGGAGGATGGGTTTATGGAGGTTGGGATTTGGTAA

Protein sequence

MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELETDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYLAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEVGIW

Homology

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BLAST of HG10014164 vs. NCBI nr
Match: XP_038896168.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida])

HSP 1 Score: 634.8 bits (1636), Expect = 4.0e-178
Identity = 314/322 (97.52%), Postives = 318/322 (98.76%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLHRQ DAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQHDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELV NDYFITE+NVGLLELAKEANSIWLDHRVPSETCSASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVGNDYFITEDNVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MNGL ISVYAPETVGCVVVD+EGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV
Sbjct: 181 MNGLRISVYAPETVGCVVVDTEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

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BLAST of HG10014164 vs. NCBI nr
Match: XP_022982271.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima])

HSP 1 Score: 615.9 bits (1587), Expect = 1.9e-172
Identity = 304/322 (94.41%), Postives = 314/322 (97.52%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TEENVG+LELA+EANSIWLD+RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

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BLAST of HG10014164 vs. NCBI nr
Match: XP_022941449.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037783.1 putative isoaspartyl peptidase/L-asparaginase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 302/322 (93.79%), Postives = 313/322 (97.20%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TEENVG+LELA+EANSIWLD+RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of HG10014164 vs. NCBI nr
Match: XP_023525179.1 (probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610.9 bits (1574), Expect = 6.3e-171
Identity = 303/323 (93.81%), Postives = 314/323 (97.21%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNSSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVP-SETCSASVETPL 180
           AFSGAEKFAR+QGVEL  NDYF+TEENVG+LELA+EANSIWLD+RVP +E CSASVETPL
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPTNEKCSASVETPL 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCG 240
           QMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCG
Sbjct: 181 QMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCG 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV C
Sbjct: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVC 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
           GFNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 GFNTTGMFRGCATEDGFMEVGIW 323

[Close]

BLAST of HG10014164 vs. NCBI nr
Match: XP_008452604.1 (PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA0064417.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa] >TYK20170.1 putative isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 607.4 bits (1565), Expect = 6.9e-170
Identity = 302/322 (93.79%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELVSNDYFITE NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy Swiss-Prot
Match: Q8GXG1 (Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g16150 PE=2 SV=2)

HSP 1 Score: 534.6 bits (1376), Expect = 7.5e-151
Identity = 260/324 (80.25%), Postives = 295/324 (91.05%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP  RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETC--SASVETP 180
           AFSGAE FAR+QGVE+V N+YF+T++NVG+L+LAKEANSI  D+R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGL+NK MGRIGDSPLIG+GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIW 323
           CGFN+ GMFRGCATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

[Close]

BLAST of HG10014164 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-100
Identity = 191/323 (59.13%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP  R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT EN+  L+ AKE N + LD+ VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of HG10014164 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-99
Identity = 193/328 (58.84%), Postives = 238/328 (72.56%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +G+ AL+S    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NP+SLARLVMDK+PH Y
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHR-----VPSETCSAS 180
           LAF GA+ FA+QQGVE V + +FIT ENV  L+LA EAN + +D+       P++   A 
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQD-DAE 180

Query: 181 VETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYA 240
            E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA
Sbjct: 181 KELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYA 240

Query: 241 CDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSN 300
            ++C VS TG+GEAII  T+AR+VAA+ME+KGL L+EA +YV+ ER  +G  GLIAVS+ 
Sbjct: 241 NELCAVSATGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAA 300

Query: 301 GEVACGFNTTGMFRGCATEDGFMEVGIW 323
           GE+A  FNTTGMFR  ATEDG+ E+ IW
Sbjct: 301 GEIAMPFNTTGMFRASATEDGYSEIAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 6.6e-99
Identity = 192/327 (58.72%), Postives = 236/327 (72.17%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +G+ AL++    +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL+TV NP+SLARLVMDK+PH Y
Sbjct: 61  NIEHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLD---HRVPSET-CSASV 180
           LAF GA+ FA+QQGVE V + + IT ENV  L+LA EAN + +D   +  P      A  
Sbjct: 121 LAFQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLTNGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
           ++C VS TG+GE IIR T+AR+VAA+ME+KGL L+EA ++VI ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-98
Identity = 191/327 (58.41%), Postives = 236/327 (72.17%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           MGGW+IA+HGGAG  P +LP  R+   ++ L  CL +GI AL++ +  +DVVELVVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
               FN+G GS LT  GTVEMEASIMDG   +CGAVSGL TV NP+SLAR VMDK+PH +
Sbjct: 61  NIQHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIF 120

Query: 121 LAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSET----CSASV 180
           LAF GA+   +QQGVE V + +FITEENV  L+LA EAN + +D+   + T      A  
Sbjct: 121 LAFQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEK 180

Query: 181 ETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYAC 240
           E PL      I      TVGCV VDS G  A+ATSTGGL+NK +GRIGD+PLIG+GTYA 
Sbjct: 181 ELPLANGDSQIG-----TVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN 240

Query: 241 DVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNG 300
           ++C VS TG+GEAIIR T+AR+VAA+ME+KGL L+EA + V+ ER  +G  GLIAVS+ G
Sbjct: 241 ELCAVSATGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAG 300

Query: 301 EVACGFNTTGMFRGCATEDGFMEVGIW 323
           E+A  FNTTGMFR CATEDG+ E+ IW
Sbjct: 301 EIAMPFNTTGMFRACATEDGYSEIAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy TrEMBL
Match: A0A6J1IYV7 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=LOC111481149 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 9.4e-173
Identity = 304/322 (94.41%), Postives = 314/322 (97.52%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TEENVG+LELA+EANSIWLD+RVP+E CSASVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of HG10014164 vs. ExPASy TrEMBL
Match: A0A6J1FND2 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446743 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.1e-172
Identity = 302/322 (93.79%), Postives = 313/322 (97.20%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPL RQ+DAK LLTRCLDLGIHALRSN SAIDVVELVVRELET
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLQRQEDAKMLLTRCLDLGIHALRSNCSAIDVVELVVRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFAR+QGVEL  NDYF+TEENVG+LELA+EANSIWLD+RVP+E CS SVETPLQ
Sbjct: 121 AFSGAEKFAREQGVELECNDYFVTEENVGMLELAREANSIWLDYRVPNEKCSTSVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NKK+GRIGDSPLIGSGTYAC VCGV
Sbjct: 181 MNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKKIGRIGDSPLIGSGTYACGVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV CG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVVCG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRGCATEDGFMEVGIW
Sbjct: 301 FNTTGMFRGCATEDGFMEVGIW 322

[Close]

BLAST of HG10014164 vs. ExPASy TrEMBL
Match: A0A5D3D9K2 (Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003020 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.4e-170
Identity = 302/322 (93.79%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELVSNDYFITE NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy TrEMBL
Match: A0A1S3BV13 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC103493576 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.4e-170
Identity = 302/322 (93.79%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAGVDPNLPLHRQDDAK+ LTRCLDLGIHALRSNHSAIDVVELVVRELE 
Sbjct: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKRFLTRCLDLGIHALRSNHSAIDVVELVVRELEN 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGL TVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLVTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQ 180
           AFSGAEKFARQQGVELVSNDYFITE NV LL+LAKEAN IWLD RVP ETC ASVETPLQ
Sbjct: 121 AFSGAEKFARQQGVELVSNDYFITEGNVELLQLAKEANGIWLDRRVPVETCCASVETPLQ 180

Query: 181 MNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGV 240
           MN +PISVYAPETVGCVVVDSEGRCAAATSTGGLINKK+GRIGDSPLIG GTYACDVCGV
Sbjct: 181 MNSVPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKVGRIGDSPLIGLGTYACDVCGV 240

Query: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACG 300
           SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEG AGLIAVSSNGEVACG
Sbjct: 241 SCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGLAGLIAVSSNGEVACG 300

Query: 301 FNTTGMFRGCATEDGFMEVGIW 323
           FNTTGMFRG ATEDGFMEV IW
Sbjct: 301 FNTTGMFRGFATEDGFMEVAIW 322

[Close]

BLAST of HG10014164 vs. ExPASy TrEMBL
Match: A0A6J1CHT1 (probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 GN=LOC111011731 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.8e-163
Identity = 288/325 (88.62%), Postives = 310/325 (95.38%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           M GWAIAVHGGAGVDPNLPL RQ++AKKLL RCL+LGI ALRS+H AIDVVELVVRELE+
Sbjct: 1   MAGWAIAVHGGAGVDPNLPLDRQEEAKKLLARCLNLGIDALRSDHPAIDVVELVVRELES 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDGPKRRCGAVSGLTTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEDGTVEMEASIMDGPKRRCGAVSGLTTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPS---ETCSASVET 180
           AFSGAE+FARQQGVE+V NDYFITEENVG+L+LAKEANSI  D+R+P+   E CSASVET
Sbjct: 121 AFSGAEEFARQQGVEMVCNDYFITEENVGMLKLAKEANSILFDYRIPNAGLEKCSASVET 180

Query: 181 PLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDV 240
           PLQMNGLPISVYAPETVGCVVVDS+GRCAAATSTGGL+NK+ GRIGDSPLIG+GTYAC +
Sbjct: 181 PLQMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRSGRIGDSPLIGAGTYACGL 240

Query: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEV 300
           CGVSCTGEGEAIIRGTLAREVAAVMEYKG+GLQEAV+YV+EERLDEGKAGLIAVSSNGEV
Sbjct: 241 CGVSCTGEGEAIIRGTLAREVAAVMEYKGMGLQEAVSYVVEERLDEGKAGLIAVSSNGEV 300

Query: 301 ACGFNTTGMFRGCATEDGFMEVGIW 323
           ACGFNTTGMFRGCATE GFMEVGIW
Sbjct: 301 ACGFNTTGMFRGCATEAGFMEVGIW 325

[Close]

BLAST of HG10014164 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 534.6 bits (1376), Expect = 5.3e-152
Identity = 260/324 (80.25%), Postives = 295/324 (91.05%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDPNLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELET 60
           MGGWAIAVHGGAG+DPNLP  RQ++AK+LLTRCL+LGI ALRSN SAIDVVELV+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 61  DPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYL 120
           DPLFNSGRGSALTE+GTVEMEASIMDG KRRCGAVSG+TTVKNP+SLARLVMDKSPHSYL
Sbjct: 61  DPLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYL 120

Query: 121 AFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETC--SASVETP 180
           AFSGAE FAR+QGVE+V N+YF+T++NVG+L+LAKEANSI  D+R+P   C  +A+ ++P
Sbjct: 121 AFSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSP 180

Query: 181 LQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVC 240
           +QMNGLPIS+YAPETVGCVVVD +G CAA TSTGGL+NK MGRIGDSPLIG+GTYA + C
Sbjct: 181 IQMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFC 240

Query: 241 GVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVA 300
           GVSCTGEGEAIIR TLAR+V+AVMEYKGL LQEAV+YVI+ RLDEG AGLIAVS+ GEV 
Sbjct: 241 GVSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVV 300

Query: 301 CGFNTTGMFRGCATEDGFMEVGIW 323
           CGFN+ GMFRGCATEDGFMEV IW
Sbjct: 301 CGFNSNGMFRGCATEDGFMEVAIW 324

[Close]

BLAST of HG10014164 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 367.9 bits (943), Expect = 8.6e-102
Identity = 191/323 (59.13%), Postives = 232/323 (71.83%), Query Frame = 0


Query: 1   MGGWAIAVHGGAGVDP-NLPLHRQDDAKKLLTRCLDLGIHALRSNHSAIDVVELVVRELE 60
           M GWAIA+HGGAG  P +LP  R+   +  L  CLDLGI AL+S    +DV ELVVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 61  TDPLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSY 120
             P FN+G+GS LT +GTVEMEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH Y
Sbjct: 61  NHPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIY 120

Query: 121 LAFSGAEKFARQQGVELVSNDYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPL 180
           LAF  AE FAR  GVE V + +FIT EN+  L+ AKE N + LD+ VPS       + P 
Sbjct: 121 LAFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPD 180

Query: 181 QMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCG 240
                 I      TVGCV VDS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C 
Sbjct: 181 NCGDSQIG-----TVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 VSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVAC 300
           +S TG+GE IIRGT+AR+VAA+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV  
Sbjct: 241 ISATGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTM 300

Query: 301 GFNTTGMFRGCATEDGFMEVGIW 323
            FNTTGMFR CA+EDG+ E+ IW
Sbjct: 301 PFNTTGMFRACASEDGYSEIAIW 312

[Close]

BLAST of HG10014164 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 4.8e-76
Identity = 144/243 (59.26%), Postives = 176/243 (72.43%), Query Frame = 0


Query: 80  MEASIMDGPKRRCGAVSGLTTVKNPVSLARLVMDKSPHSYLAFSGAEKFARQQGVELVSN 139
           MEASIMDG  +RCGAVSGLTTV NP+SLARLVM+K+PH YLAF  AE FAR  GVE V +
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 DYFITEENVGLLELAKEANSIWLDHRVPSETCSASVETPLQMNGLPISVYAPETVGCVVV 199
            +FIT EN+  L+ AKE N + LD+ VPS       + P       I      TVGCV V
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSP------KVPDNCGDSQIG-----TVGCVAV 120

Query: 200 DSEGRCAAATSTGGLINKKMGRIGDSPLIGSGTYACDVCGVSCTGEGEAIIRGTLAREVA 259
           DS G  A+ATSTGG +NK +GRIGD+P+IG+GTYA  +C +S TG+GE IIRGT+AR+VA
Sbjct: 121 DSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVA 180

Query: 260 AVMEYKGLGLQEAVNYVIEERLDEGKAGLIAVSSNGEVACGFNTTGMFRGCATEDGFMEV 319
           A+MEYKGL L EA  YV+++ +  G  GL+AVS+NGEV   FNTTGMFR CA+EDG+ E+
Sbjct: 181 ALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEI 232

Query: 320 GIW 323
            IW
Sbjct: 241 AIW 232

[Close]

BLAST of HG10014164 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 1.5e-21
Identity = 92/339 (27.14%), Postives = 143/339 (42.18%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLHRQDDAKKLLT----RCLDLGIHALRSNHSAIDVVELVVRELETD 65
           +AVH GAG       H   + K L +     CL       + +   ID V   ++ LE D
Sbjct: 15  VAVHVGAG------YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDD 74

Query: 66  PLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVSLARL----------V 125
           P  N+GRGS LTE+G VE +AS+MDG     G V  +  V+N + +A L          +
Sbjct: 75  PSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTL 134

Query: 126 MDKSPHSYLAFSGAEKFARQQ-----GVELVSNDYFITEENVGLLELAKE------ANSI 185
           + + P   L   GA ++ + +     G    ++ + +TE         K       A SI
Sbjct: 135 LGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSI 194

Query: 186 WLDHRVP-----SETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLI 245
                 P     +ETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+ 
Sbjct: 195 LSAEEHPRGTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIA 254

Query: 246 NKKMGRIGDSPLIGSGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGL 298
            K  GR+G +   GSG +A          + G   +G GE ++RG  ARE    +     
Sbjct: 255 MKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQA 314

[Close]

BLAST of HG10014164 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 1.2e-18
Identity = 92/331 (27.79%), Postives = 142/331 (42.90%), Query Frame = 0


Query: 6   IAVHGGAGVDPNLPLHRQDDAKKLLT----RCLDLGIHALRSNHSAIDVVELVVRELETD 65
           +AVH GAG       H   + K L +     CL       + +   ID V   ++ LE D
Sbjct: 15  VAVHVGAG------YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDD 74

Query: 66  PLFNSGRGSALTEEGTVEMEASIMDGPKRRCGAVSGLTTVKNPVS--LARLVMDKSPHSY 125
           P  N+GRGS LTE+G VE +AS+MDG     G   G+  V + VS  L++L++       
Sbjct: 75  PSTNAGRGSNLTEDGHVECDASLMDGDS---GMFGGVGAVPDQVSEMLSKLLL----CWL 134

Query: 126 LAFSGAEKFARQQ-----GVELVSNDYFITEENVGLLELAKE------ANSIWLDHRVP- 185
           L   GA ++ + +     G    ++ + +TE         K       A SI      P 
Sbjct: 135 LVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPR 194

Query: 186 ----SETCSASVETPLQMNGLPISVYAPETVGCVVVDSEGRCAAATSTGGLINKKMGRIG 245
               +ETC  +V +    +   I     +TVG + VD+EG  A  +S+GG+  K  GR+G
Sbjct: 195 GTENNETCEENVSSCAAADEDKIM----DTVGVICVDNEGHIACGSSSGGIAMKISGRVG 254

Query: 246 DSPLIGSGTYACD--------VCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVNY 298
            +   GSG +A          + G   +G GE ++RG  ARE    +     G   A   
Sbjct: 255 LAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMK 314

The following BLAST results are available for this feature:

BLAST of HG10014164 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896168.1	4.0e-178	97.52	probable isoaspartyl peptidase/L-asparaginase 2 [Benincasa hispida]	[more]
XP_022982271.1	1.9e-172	94.41	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita maxima]	[more]
XP_022941449.1	1.3e-171	93.79	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita moschata] >KAG6608447...	[more]
XP_023525179.1	6.3e-171	93.81	probable isoaspartyl peptidase/L-asparaginase 2 [Cucurbita pepo subsp. pepo]	[more]
XP_008452604.1	6.9e-170	93.79	PREDICTED: probable isoaspartyl peptidase/L-asparaginase 2 [Cucumis melo] >KAA00...	[more]

BLAST of HG10014164 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXG1	7.5e-151	80.25	Probable isoaspartyl peptidase/L-asparaginase 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
P50287	1.2e-100	59.13	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
P30364	1.7e-99	58.84	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
Q9ZSD6	6.6e-99	58.72	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P50288	1.1e-98	58.41	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]

BLAST of HG10014164 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYV7	9.4e-173	94.41	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FND2	6.1e-172	93.79	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5D3D9K2	3.4e-170	93.79	Putative isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BV13	3.4e-170	93.79	probable isoaspartyl peptidase/L-asparaginase 2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1CHT1	6.8e-163	88.62	probable isoaspartyl peptidase/L-asparaginase 2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of HG10014164 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16150.1	5.3e-152	80.25	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.1	8.6e-102	59.13	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	4.8e-76	59.26	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	1.5e-21	27.14	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	1.2e-18	27.79	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 4..322 e-value: 8.1E-103 score: 343.7
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 1..322
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 193..322 e-value: 1.9E-43 score: 149.7
None	No IPR available	PIRSR	PIRSR600246-2	PIRSR600246-2	coord: 3..317 e-value: 1.2E-92 score: 308.3
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 3..317 e-value: 1.2E-92 score: 308.3
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 3..317 e-value: 1.2E-92 score: 308.3
None	No IPR available	PANTHER	PTHR10188:SF36	PEPTIDASE T2, ASPARAGINASE 2, NUCLEOPHILE AMINOHYDROLASE-RELATED	coord: 1..322
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 5..315 e-value: 1.09402E-121 score: 348.297
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 4..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014164.1	HG10014164.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity
molecular_function	GO:0016787	hydrolase activity