HG10014134 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014134 vs. NCBI nr
Match: XP_038896694.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 411.4 bits (1056), Expect = 6.5e-111
Identity = 204/291 (70.10%), Postives = 242/291 (83.16%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQD---Q 60
           MFEN SKD  VTVVAFRGTSV + +DW VD NF + KL+GVG IH+GFMQALG+QD    
Sbjct: 196 MFENTSKDPKVTVVAFRGTSVLEPSDWTVDFNFSWSKLEGVGNIHAGFMQALGFQDLAGW 255

Query: 61  EIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTIL 120
             D  +    +AY TL  KLK IA++NDNA+FIITGHSLGGALATLFV+LLA++NETTIL
Sbjct: 256 PKDLLQPKHQYAYYTLRQKLKDIAKANDNAEFIITGHSLGGALATLFVTLLAHHNETTIL 315

Query: 121 HKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFG 180
           HK++AVYTYGQ RVG+Q FA+FMV T +RY  +YYRYVYSFD++PRVPFDS+V  YKHFG
Sbjct: 316 HKIRAVYTYGQPRVGNQEFAQFMVDTVERYGFKYYRYVYSFDLVPRVPFDSVVFKYKHFG 375

Query: 181 GCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFT 240
           GCVYFDCFY  KFL++QPNTNYFS IWLIPKYL ACWE +RSLII PI+KG+D+FEG FT
Sbjct: 376 GCVYFDCFYHGKFLKEQPNTNYFSPIWLIPKYLSACWEFIRSLIITPIVKGRDYFEGFFT 435

Query: 241 IMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
           I+ER LGLLIPG+SAHVCSNYVN+TRLG I L  D+ ETL +LA +++A+Y
Sbjct: 436 ILERTLGLLIPGISAHVCSNYVNVTRLGNIQLPSDYEETL-QLAHYIDANY 485

BLAST of HG10014134 vs. NCBI nr
Match: XP_031739748.1 (uncharacterized protein LOC105435389 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 3.1e-105
Identity = 200/294 (68.03%), Postives = 238/294 (80.95%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ----- 60
           MFEN  KD  VTV+AFRGTSV D+NDWMVD +F +  L+G   IHSGFMQALGYQ     
Sbjct: 208 MFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGW 267

Query: 61  DQEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETT 120
            +E+  D  H  FAY  L   L+ IA+SNDNAKFIITGHSLGGALATLFV+LLAY+NET 
Sbjct: 268 PKELT-DPKH-EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETI 327

Query: 121 ILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVT-NYK 180
           +L K+QAVYT+GQ RVG+Q+FA+FMV TFK +DI+YYRYVYSFD++PR+PF S+   +Y+
Sbjct: 328 LLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYR 387

Query: 181 HFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEG 240
           HFGGCVYFD FY  KFL++QPNTNYFSLIW+IPKYL A WE +RSLII PI+KG+ +FEG
Sbjct: 388 HFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEG 447

Query: 241 LFTIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
            FTIMER +GL+IPG+SAHVCSNYVNLTR G IHL PDH+E L K A ++EADY
Sbjct: 448 FFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELL-KFAHYIEADY 498

BLAST of HG10014134 vs. NCBI nr
Match: KGN55200.1 (hypothetical protein Csa_012841 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 3.1e-105
Identity = 200/294 (68.03%), Postives = 238/294 (80.95%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ----- 60
           MFEN  KD  VTV+AFRGTSV D+NDWMVD +F +  L+G   IHSGFMQALGYQ     
Sbjct: 197 MFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGW 256

Query: 61  DQEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETT 120
            +E+  D  H  FAY  L   L+ IA+SNDNAKFIITGHSLGGALATLFV+LLAY+NET 
Sbjct: 257 PKELT-DPKH-EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETI 316

Query: 121 ILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVT-NYK 180
           +L K+QAVYT+GQ RVG+Q+FA+FMV TFK +DI+YYRYVYSFD++PR+PF S+   +Y+
Sbjct: 317 LLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYR 376

Query: 181 HFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEG 240
           HFGGCVYFD FY  KFL++QPNTNYFSLIW+IPKYL A WE +RSLII PI+KG+ +FEG
Sbjct: 377 HFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEG 436

Query: 241 LFTIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
            FTIMER +GL+IPG+SAHVCSNYVNLTR G IHL PDH+E L K A ++EADY
Sbjct: 437 FFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELL-KFAHYIEADY 487

BLAST of HG10014134 vs. NCBI nr
Match: XP_022936593.1 (uncharacterized protein LOC111443149 [Cucurbita moschata])

HSP 1 Score: 384.0 bits (985), Expect = 1.1e-102
Identity = 187/290 (64.48%), Postives = 232/290 (80.00%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQDQ--- 60
           MF+N +KD  VTVVAFRGTSVF+ +DWMVD N  + K++G+G+IHSGFMQALG Q     
Sbjct: 185 MFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRIHSGFMQALGLQKATGW 244

Query: 61  EIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTIL 120
             +  E    FAY TL  +L+ I +SND AKFIITGHSLGGALATLFV+LLA + ++TIL
Sbjct: 245 PKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGALATLFVTLLALHQDSTIL 304

Query: 121 HKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFG 180
            +LQAVYT+GQ RVGD++FAEFMV T  RY ++YYRYVYSFD++PRVPFDS++  YKHFG
Sbjct: 305 QRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDLVPRVPFDSIIFKYKHFG 364

Query: 181 GCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFT 240
           GC+YFDCFY  KF ++QPNTNYFSLIW+IPKY  A WEL+ SLIIIP+ KG+++FEG  T
Sbjct: 365 GCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISSLIIIPVFKGREYFEGFVT 424

Query: 241 IMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEAD 286
            +ER++GL  PGLSAH+C NYVN+TR G I +LPD NETL KL +++EAD
Sbjct: 425 TLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETL-KLVRYIEAD 473

BLAST of HG10014134 vs. NCBI nr
Match: KAG6591383.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-102
Identity = 188/290 (64.83%), Postives = 231/290 (79.66%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQDQEID 60
           MF+N +KD  VTVVAFRGTSVF+ +DWMVD N  + K++G+G+IHSGFMQALG Q     
Sbjct: 185 MFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRIHSGFMQALGLQKATGW 244

Query: 61  HDETHG---NFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTIL 120
             E  G    FAY TL  +L+ I +SND AKFI+TGHSLGGALATLFV+LLA + ++TIL
Sbjct: 245 PKELPGAEHEFAYYTLRQRLRDIVKSNDKAKFIMTGHSLGGALATLFVTLLALHQDSTIL 304

Query: 121 HKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFG 180
            +LQAVYT+GQ RVGD++FAEFMV T  RY  +YYRYVYSFD++PRVPFDS++  YKHFG
Sbjct: 305 QRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGFKYYRYVYSFDLVPRVPFDSIIFKYKHFG 364

Query: 181 GCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFT 240
           GC+YFDCFY  KF  +QPNTNYFSLIW+IPKYL A WEL+ SLIIIP+ KG+++FEG  T
Sbjct: 365 GCLYFDCFYNGKFRREQPNTNYFSLIWVIPKYLSAHWELISSLIIIPVFKGREYFEGFVT 424

Query: 241 IMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEAD 286
            +ER++GL  PGLSAH+C NYVN+TR G I +LPD NETL KL +++EAD
Sbjct: 425 TLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETL-KLVRYIEAD 473

BLAST of HG10014134 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.6e-51
Identity = 108/286 (37.76%), Postives = 169/286 (59.09%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALG------- 60
           +F +K KD  + V++FRGT  FD +DW  D ++ + ++  VG++H GF++A+G       
Sbjct: 224 VFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDT 283

Query: 61  -------YQDQEIDHDETHGNF-------AYNTLALKLKTIAESNDNAKFIITGHSLGGA 120
                  ++    + + +  N        AY  + + LK +   ++NA+F++TGHSLGGA
Sbjct: 284 TTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGA 343

Query: 121 LATLFVSLLAYNNETTILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFD 180
           LA LF +LL  N ET I+ +L  VYT+GQ R+G++    FM     +   +Y+R VY  D
Sbjct: 344 LAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCND 403

Query: 181 MIPRVPFDSMVTNYKHFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRS 240
           ++PR+P+D     YKHFG C+++D FY     E +P+ N + L + I  ++IA WEL+R 
Sbjct: 404 IVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRG 463

Query: 241 LIIIPIMKGKDHFEGLFTIMERMLGLLIPGLSAHVCSNYVNLTRLG 264
           L  +    G D+ EG F I+ R++GL+IPGLS H  ++YVN  RLG
Sbjct: 464 L-TMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLG 508

BLAST of HG10014134 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 106/321 (33.02%), Postives = 164/321 (51.09%), Query Frame = 0

Query: 5   KSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGY------------ 64
           K  ++ +V+FRGT  FD +DW+ D ++ + ++  +G++H GF++ALG             
Sbjct: 243 KDANLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQ 302

Query: 65  ------------------------------------------QDQEIDHDETHGNF---- 124
                                                      D E   D     F    
Sbjct: 303 LFVNNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEI 362

Query: 125 ----AYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQAVY 184
               AY  +  KLK + + + NAKF++TGHSLGGALA LF ++L  + E  ++ +L  +Y
Sbjct: 363 PERTAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIY 422

Query: 185 TYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFGGCVYFDC 244
           TYGQ RVG++    FM    +    +Y+R VY  D++PR+P+D+    +KHFG C Y++ 
Sbjct: 423 TYGQPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNS 482

Query: 245 FYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFTIMERMLG 264
            Y  + + ++PN NYF + +L+P YL A WEL+RS   +  M G ++ E   ++M R LG
Sbjct: 483 LYIEQNINEEPNPNYFGMRFLVPLYLNAGWELIRS-FTMGYMYGSEYEECWESVMLRALG 542

BLAST of HG10014134 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.8e-45
Identity = 110/309 (35.60%), Postives = 164/309 (53.07%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGY------ 60
           +F +K KD  + V++FRGT  F + +W  D +F    L   G +H GF++A+G       
Sbjct: 219 IFCDKPKDANLIVISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSI 278

Query: 61  ---------------------QDQEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITG 120
                                 +   DH     +  Y   A  LK + + + NAKF++TG
Sbjct: 279 SSFETSINTKSPGSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTG 338

Query: 121 HSLGGALATLFVSLLAYNNETTILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYR 180
           HSLGGALA LF  +L    ET +L +L  VYT+GQ R+G+ N   FM       + +Y+R
Sbjct: 339 HSLGGALAILFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFR 398

Query: 181 YVYSFDMIPRVPFDSMVTNYKHFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIAC 240
            VY  DM+PRVPFD +   ++HFG C+Y+D  +   F +++P+ N F +   I  ++ A 
Sbjct: 399 VVYCNDMVPRVPFDDVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAW 458

Query: 241 WELLRSLIIIPIMKGKDHFEGLFTIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDH 281
           WEL RS I+  +  G ++ E   + M R+LGL +PG++AH   NYVN  RLG    +P  
Sbjct: 459 WELWRSFILNHVY-GAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLGRELAIP-- 518

BLAST of HG10014134 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.7e-08
Identity = 48/161 (29.81%), Postives = 73/161 (45.34%), Query Frame = 0

Query: 6   SKDVTVVAFRGTSVFDLNDWMVDSNFL---FKKLDGVGQIHSGFMQALGYQDQEIDHDET 65
           S+    + FRG+S   + +W+ D  F+   +  + G  ++H GF+ + G           
Sbjct: 165 SEKTIYIVFRGSS--SIRNWIADLTFVPVSYPPVSGT-KVHKGFLDSYGE---------- 224

Query: 66  HGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQAVY 125
                 N L   +    +   + K  +TGHSLGGA A L  +L  Y  E  +      +Y
Sbjct: 225 ----VQNELVATVLDQFKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSSSNLFLY 284

Query: 126 TYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVP 164
           T GQ RVGD  FA ++V T     I Y R V   D++P +P
Sbjct: 285 TQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP 303

BLAST of HG10014134 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.6e-06
Identity = 43/163 (26.38%), Postives = 79/163 (48.47%), Query Frame = 0

Query: 4   NKSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGV---GQIHSGFMQALGYQDQEIDHD 63
           + +  + V++FRG+    + +W+ + NF  K+++ +    + H GF  +           
Sbjct: 92  DNTNKLIVLSFRGSR--SIENWIGNLNFDLKEINDICSGCRGHDGFTSS----------- 151

Query: 64  ETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQA 123
                   +TL  K++     + + + + TGHSLGGALAT+  + L  N           
Sbjct: 152 ---WRSVADTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------D 211

Query: 124 VYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVP 164
           V++YG  RVG++ FAEF+     +     YR  ++ D++PR+P
Sbjct: 212 VFSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTNDIVPRLP 229

BLAST of HG10014134 vs. ExPASy TrEMBL
Match: A0A0A0KZS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.5e-105
Identity = 200/294 (68.03%), Postives = 238/294 (80.95%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ----- 60
           MFEN  KD  VTV+AFRGTSV D+NDWMVD +F +  L+G   IHSGFMQALGYQ     
Sbjct: 197 MFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGW 256

Query: 61  DQEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETT 120
            +E+  D  H  FAY  L   L+ IA+SNDNAKFIITGHSLGGALATLFV+LLAY+NET 
Sbjct: 257 PKELT-DPKH-EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETI 316

Query: 121 ILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVT-NYK 180
           +L K+QAVYT+GQ RVG+Q+FA+FMV TFK +DI+YYRYVYSFD++PR+PF S+   +Y+
Sbjct: 317 LLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYR 376

Query: 181 HFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEG 240
           HFGGCVYFD FY  KFL++QPNTNYFSLIW+IPKYL A WE +RSLII PI+KG+ +FEG
Sbjct: 377 HFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEG 436

Query: 241 LFTIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
            FTIMER +GL+IPG+SAHVCSNYVNLTR G IHL PDH+E L K A ++EADY
Sbjct: 437 FFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELL-KFAHYIEADY 487

BLAST of HG10014134 vs. ExPASy TrEMBL
Match: A0A6J1FDN5 (uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC111443149 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 5.4e-103
Identity = 187/290 (64.48%), Postives = 232/290 (80.00%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQDQ--- 60
           MF+N +KD  VTVVAFRGTSVF+ +DWMVD N  + K++G+G+IHSGFMQALG Q     
Sbjct: 185 MFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRIHSGFMQALGLQKATGW 244

Query: 61  EIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTIL 120
             +  E    FAY TL  +L+ I +SND AKFIITGHSLGGALATLFV+LLA + ++TIL
Sbjct: 245 PKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGALATLFVTLLALHQDSTIL 304

Query: 121 HKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFG 180
            +LQAVYT+GQ RVGD++FAEFMV T  RY ++YYRYVYSFD++PRVPFDS++  YKHFG
Sbjct: 305 QRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDLVPRVPFDSIIFKYKHFG 364

Query: 181 GCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFT 240
           GC+YFDCFY  KF ++QPNTNYFSLIW+IPKY  A WEL+ SLIIIP+ KG+++FEG  T
Sbjct: 365 GCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISSLIIIPVFKGREYFEGFVT 424

Query: 241 IMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEAD 286
            +ER++GL  PGLSAH+C NYVN+TR G I +LPD NETL KL +++EAD
Sbjct: 425 TLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETL-KLVRYIEAD 473

BLAST of HG10014134 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 6.4e-80
Identity = 155/280 (55.36%), Postives = 205/280 (73.21%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ----- 60
           MF+N + D  VTVVAFRGTS  D  DW VD++F +  ++GVG+IHSGFM+ALG Q     
Sbjct: 197 MFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRIHSGFMKALGLQKATGW 256

Query: 61  DQEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETT 120
            +E+   + H  FAY TL  KL+ IA+SNDNAKFIITGHSLGGALA LFV++L+ + ++ 
Sbjct: 257 PEELVKSKHH--FAYYTLRQKLRDIAKSNDNAKFIITGHSLGGALAALFVTVLSLHRDSA 316

Query: 121 ILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKH 180
           +L KLQAVYTY Q RVGD+ FAEFMV T K+Y  +Y+RYVYS D++PRVPFD ++  YKH
Sbjct: 317 VLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSDLVPRVPFDGVIFKYKH 376

Query: 181 FGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGL 240
           FG C+YF+  Y  + ++++PN NYFSLIW++PKYL A WEL+RS  I+P++KG D+ E L
Sbjct: 377 FGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRS-FIVPLVKGFDYHESL 436

Query: 241 FTIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNE 274
                R++GL IPGL+AH+ ++YVN  RLG  +  P   E
Sbjct: 437 LMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPPPQEFE 473

BLAST of HG10014134 vs. ExPASy TrEMBL
Match: A0A5D3D9F3 (Lipase class 3 family protein-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003310 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.9e-79
Identity = 158/292 (54.11%), Postives = 213/292 (72.95%), Query Frame = 0

Query: 2   FENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ-----D 61
           FEN + D  V +VAFRGTS  D  DW VD +  + +++GVG+IHSGFM+ALG Q      
Sbjct: 134 FENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFMKALGLQKATGWP 193

Query: 62  QEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTI 121
           +E+   +TH  FAY TL  +L+ IA++N+ A+FI TGHSLGGALATLFV++L+ ++E+TI
Sbjct: 194 KELPKTQTH-EFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLSLHDESTI 253

Query: 122 LHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHF 181
           L KL +VYTYGQ RVGDQ FA+FMV   ++Y  +Y+RYVYS+D++PRVP D+++  YKHF
Sbjct: 254 LEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHF 313

Query: 182 GGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLF 241
           G CVYF+  Y  + +++QPN NYFSL+W+IPKYL A WEL+RS  IIP +KG D++E L 
Sbjct: 314 GRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRS-FIIPFVKGFDYYESLL 373

Query: 242 TIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
               R++GLLIPGLSAH   NYVN+TRLG ++ +PD  E    L   +E D+
Sbjct: 374 MKGARLVGLLIPGLSAHFPLNYVNVTRLGKLN-VPDEVED-PFLGYEIEDDF 421

BLAST of HG10014134 vs. ExPASy TrEMBL
Match: A0A1S3BUY0 (uncharacterized protein LOC103493549 OS=Cucumis melo OX=3656 GN=LOC103493549 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.9e-79
Identity = 158/292 (54.11%), Postives = 213/292 (72.95%), Query Frame = 0

Query: 2   FENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQ-----D 61
           FEN + D  V +VAFRGTS  D  DW VD +  + +++GVG+IHSGFM+ALG Q      
Sbjct: 196 FENTAIDPNVVIVAFRGTSPLDAYDWQVDVDLSWYEIEGVGRIHSGFMKALGLQKATGWP 255

Query: 62  QEIDHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTI 121
           +E+   +TH  FAY TL  +L+ IA++N+ A+FI TGHSLGGALATLFV++L+ ++E+TI
Sbjct: 256 KELPKTQTH-EFAYYTLRKQLRDIAKANEKARFIFTGHSLGGALATLFVTVLSLHDESTI 315

Query: 122 LHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHF 181
           L KL +VYTYGQ RVGDQ FA+FMV   ++Y  +Y+RYVYS+D++PRVP D+++  YKHF
Sbjct: 316 LEKLDSVYTYGQPRVGDQQFAKFMVNCVQKYGFKYHRYVYSYDLVPRVPSDAVLFKYKHF 375

Query: 182 GGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLF 241
           G CVYF+  Y  + +++QPN NYFSL+W+IPKYL A WEL+RS  IIP +KG D++E L 
Sbjct: 376 GRCVYFNSLYKGRIVKEQPNRNYFSLLWVIPKYLSAWWELIRS-FIIPFVKGFDYYESLL 435

Query: 242 TIMERMLGLLIPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLAQHVEADY 287
               R++GLLIPGLSAH   NYVN+TRLG ++ +PD  E    L   +E D+
Sbjct: 436 MKGARLVGLLIPGLSAHFPLNYVNVTRLGKLN-VPDEVED-PFLGYEIEDDF 483

BLAST of HG10014134 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 9.5e-68
Identity = 123/263 (46.77%), Postives = 177/263 (67.30%), Query Frame = 0

Query: 8   DVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQDQ----EIDHDETH 67
           ++ +V+FRGT  FD +DW  D +  + ++  VG+IH GFM+ALG Q +    E++ DET 
Sbjct: 198 NLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGLQKEGWPKEVNLDETQ 257

Query: 68  GN---FAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQA 127
                +AY T+   LK I + N  +KFI+TGHSLGGALA LF ++L  ++E  +L +L+ 
Sbjct: 258 NATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAVLVMHDEEQMLERLEG 317

Query: 128 VYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFGGCVYF 187
           VYT+GQ RVGD+ F  FM  + K++D++Y RYVY  DM+PR+PFD     +KHFG C+Y+
Sbjct: 318 VYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFDDKTLMFKHFGACLYY 377

Query: 188 DCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFTIMERM 247
           D FY  K  E++PN NYF+L+W++PK + A WEL+RS  ++P  KG +  EG F    R+
Sbjct: 378 DSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRS-FVMPYWKGGEFREGWFLRCFRV 437

Query: 248 LGLLIPGLSAHVCSNYVNLTRLG 264
           + LLIPGL AH  + Y+N+T LG
Sbjct: 438 VALLIPGLPAHFPNEYINVTLLG 459

BLAST of HG10014134 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 241.9 bits (616), Expect = 6.4e-64
Identity = 120/263 (45.63%), Postives = 172/263 (65.40%), Query Frame = 0

Query: 8   DVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQD----QEIDHDETH 67
           ++ VV+FRGT  F+ +DW  D +  +  +  VG+IH GFM+ALG       +EI+ D+T 
Sbjct: 200 NLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALGLPKEGWYEEINSDQTQ 259

Query: 68  ---GNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQA 127
                 AY T+  +LK + E N  +KFI++GHSLGGALA LF ++L  ++E  +L +L+ 
Sbjct: 260 NKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAVLIMHDEKEMLERLEG 319

Query: 128 VYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFGGCVYF 187
           VYT+GQ RVGD++F  +M    K +D++Y RYVY  DM+PR+PFD     +KHFGGC+Y 
Sbjct: 320 VYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFDDKTLMFKHFGGCLYC 379

Query: 188 DCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFTIMERM 247
           D FY  K  E++PN NYF++ W+IPK + A WEL+RS  II   +G+++ EG      R+
Sbjct: 380 DSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRS-FIISCWQGREYREGWLLTCFRL 439

Query: 248 LGLLIPGLSAHVCSNYVNLTRLG 264
           + LLIPGL AH  + YVN+  LG
Sbjct: 440 VALLIPGLPAHFPNEYVNVALLG 461

BLAST of HG10014134 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 3.1e-58
Identity = 112/265 (42.26%), Postives = 165/265 (62.26%), Query Frame = 0

Query: 4   NKSKDVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQD------QEI 63
           N  +   VVAFRGT +F+  DW  D +  + +L  +G IH GFM+ALG Q+      + +
Sbjct: 199 NGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNIHGGFMKALGLQNNCSWPKEPL 258

Query: 64  DHDETHGNFAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHK 123
            + +     AY ++   LKT+   N N KF++TGHSLGGALA LF ++L  ++ET +L +
Sbjct: 259 SNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLVIHHETELLER 318

Query: 124 LQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPFDSMVTNYKHFGGC 183
           +Q VYTYGQ RVGD  F EFM K  ++Y+I+YYR+VY+ D++PR+P+D     +KHFG C
Sbjct: 319 IQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDKDLMFKHFGTC 378

Query: 184 VYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFTIM 243
           +Y+D  Y +K + +Q + N+F L  +I     A  E +RS  I+   KG ++ EG     
Sbjct: 379 IYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIV-AEKGSEYSEGWLLKG 438

Query: 244 ERMLGLLIPGLSAHVCSNYVNLTRL 263
            R LG+++PG+S H   +YVN TRL
Sbjct: 439 GRALGIIVPGVSNHTPQDYVNATRL 462

BLAST of HG10014134 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 218.8 bits (556), Expect = 5.8e-57
Identity = 116/275 (42.18%), Postives = 166/275 (60.36%), Query Frame = 0

Query: 8   DVTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALGYQDQEIDHDETH--GN 67
           D+ VV+FRGT  F+  DW  D +  + ++  VG++H+GF +ALG Q      +       
Sbjct: 203 DLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRALGLQKDGWPKENISLLHQ 262

Query: 68  FAYNTLALKLKTIAESNDNAKFIITGHSLGGALATLFVSLLAYNNETTILHKLQAVYTYG 127
           +AY T+   L+     N N K+I+TGHSLGGALA LF ++LA + E  +L KL+ +YT+G
Sbjct: 263 YAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFG 322

Query: 128 QLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFDMIPRVPF-DSMVTNYKHFGGCVYFDCFY 187
           Q RVGD++F EFM    K++ I+Y R+VY+ D++PRVPF D  + +YKH+G C  F+  Y
Sbjct: 323 QPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLY 382

Query: 188 CSKFLEQQPNTNYFSLIWLIPKYLIACWELLRSLIIIPIMKGKDHFEGLFTIMERMLGLL 247
             K  E  PN NYF+L+WLIP+ L   WE +RS  I+   KG ++ E       R++G++
Sbjct: 383 KGKVREDAPNANYFNLLWLIPQLLTGLWEFIRS-FILQFWKGDEYKENWLMRFVRVVGIV 442

Query: 248 IPGLSAHVCSNYVNLTRLGIIHLLPDHNETLKKLA 280
            PG S H   +YVN TRLG +   P       KLA
Sbjct: 443 FPGGSNHFPFDYVNSTRLGGLVRPPPTTTPEDKLA 476

BLAST of HG10014134 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.3e-52
Identity = 108/286 (37.76%), Postives = 169/286 (59.09%), Query Frame = 0

Query: 1   MFENKSKD--VTVVAFRGTSVFDLNDWMVDSNFLFKKLDGVGQIHSGFMQALG------- 60
           +F +K KD  + V++FRGT  FD +DW  D ++ + ++  VG++H GF++A+G       
Sbjct: 224 VFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDT 283

Query: 61  -------YQDQEIDHDETHGNF-------AYNTLALKLKTIAESNDNAKFIITGHSLGGA 120
                  ++    + + +  N        AY  + + LK +   ++NA+F++TGHSLGGA
Sbjct: 284 TTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGA 343

Query: 121 LATLFVSLLAYNNETTILHKLQAVYTYGQLRVGDQNFAEFMVKTFKRYDIQYYRYVYSFD 180
           LA LF +LL  N ET I+ +L  VYT+GQ R+G++    FM     +   +Y+R VY  D
Sbjct: 344 LAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCND 403

Query: 181 MIPRVPFDSMVTNYKHFGGCVYFDCFYCSKFLEQQPNTNYFSLIWLIPKYLIACWELLRS 240
           ++PR+P+D     YKHFG C+++D FY     E +P+ N + L + I  ++IA WEL+R 
Sbjct: 404 IVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRG 463

Query: 241 LIIIPIMKGKDHFEGLFTIMERMLGLLIPGLSAHVCSNYVNLTRLG 264
           L  +    G D+ EG F I+ R++GL+IPGLS H  ++YVN  RLG
Sbjct: 464 L-TMGYTHGPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLG 508