HG10014129 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10014129
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionTrimethylguanosine synthase
LocationChr02: 7818030 .. 7825157 (+)
RNA-Seq ExpressionHG10014129
SyntenyHG10014129
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGTAACCACCCTTCCTCACCTCCATTCTCGCCGGAAGATTAGGGAAATCGAAACTAGAAGAAAACAGCTGATTTTATTTTAACCGCAGTGTTCTGTTCGACTTCTCTTTGATTTCATTTCACTTGTTAGGGCATTGACGACAATTTCTAATGGTTGCGTTACAGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGTAAGTCTCTAATTTCACTGTTTTGCGACATTGATACTTATTGAGTTCTCTTTGCTCCCTGGTTACTTTACAATCTACTAATTTCTTTGTTTGTTTTAACAAGTTAGCTCTTGATGCTGATGATGCCAATAACGAAAAATTCGGGGAGAAAATCTGTACTACTATCAGTGGCAAGTGTTTCATTCGTCAATCTCTTTGTTTGCCTAGCTTTATTTTACTTTTTTCAAGTTATGAGATATTTGTGGCAAAATTTGATGTTCACCCACTATTTCTGTGGCATAAACATGCATGTTGATTGGTGCTAAAGGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGGTACTACAATATACTACTTCTGTATATTATTTAGGTTAAATCATGATATTTTTCCTCTAATCAACATGTTGATACTCCTCTACCTCTAGAAGTATGTTTTTAATTAATTAATTTCAACGGTCTTTATCTTCAGTGAATGTTAAAAAACGCTGAACCACATCGCTGAACTATACATCAATGTTGTATCAATTCTACCTTGGTACCACGTTAAGGTATAATTTAACCCATTACATAAATAAGCTATTCCACATTTTTTTCTCCATTCTTTTGGCCATATCCTCATATAGTTATTGAAAATTAACATAGAGTAACTGTCGTGGCTCTATCATATCTTTTGTTTCATTTCTTCTAGAACAATTGCCAGTACTTAGATGAGAGCCGTATATTGGATAGCTAAATGGATTGAAATGTTTTTATGCATTACCATAGCCATTTGGTGGAAATACTTTTTTCTGCATTTATCCATATTTGTGTGATGTTTTGCCATGTAGCAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGGTTCTTGCCTCTTGTCTAGGCTTTCTATTTCTGATTTTCTCTTTTACCTATATGAGTAATTAGTTTGGAGAGGTATATTGTTTACTATTGCAATTTCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGGTAGCATTTAGAAACAGAATTTTTATTGGCGCCTATTAGCTTTCCTTATCTCACCACAAATTACTCTTTTTGTTTGGGCTCCCTTGCTGTGTTTCCAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAAATAGGAAAAAGAAAATGAAAAGAACACGCAGACGTGGACAACTATCTGATAGAAATGAAGGTTTATTTTTTCTGTTTTGCTATGCCTGTCAAATACCCTTTTTTTTAAAAAAAAATTATTTTGAATTTATATTTTTTTATCAATAGACATTTTATTCACGCTCCACTCCATCAAGTTTCATCAATTTGTTGGGTCTGTTATTTATTAAAATATGACACATTTCCTTTGATTGCGACCTTCTTTTCTATGGTCCAATACATTGATTCTTATTCACAATTTATTTTTGTTCTGAAGATTGAAGATCATAAGAACTTGACACTCAAGTTAAAAATTTAGATTATTCCTTAACATATGGGATATAAAATATGTAACTGTGCCATATATAGTCAATTTCAGTTCTATGCAATGCAGATAGGAACAGGAGATCAGAGTTTAGATTACCCTATACATCAAGAATCTGAAATAGAAGTATGAGCTTTTGTCTTAGTAACTTAATGTTGATCTATCTGAGAGGAAACAAATAAATTCATGGGTAAATATTGTGTTTTCCCTTCCTCTTGGAACAAGTTTTCATTTGATGTACTTCTGCGAAAATAGTGGTTTGGTGCTTGCTTCGGGATTATGAACCTTATTTAGAGCTTTAGTAATATTCAACAACATGAGCCTGGCAAATATTGTAGAAGAACAATTTTGGCTGGTGGTGCTTTCTGGTTGTAACTTATAACATAACTGCTACTGCCAAATTACACTCTTTCGGTAGCAGATTAGATTTCTTAGTAAGCCTCATGATAGAAATAGATAATGACATGCCATAAGATTCTTTTCTCCCTGACAGACAAATTTTCTTCTGCAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGTGTTGTTCGATTTATTTTTTATTCATAAGTTTGGGGGGATAATTGACACTTTAATGCATCTTATAAGCTCAGATAATTGGTTAGGTTGTTGTGTAGTTAATTTACACGTGTATCAGTGGTTGATGAATAGGTGGATCTGATCAGATTCATGTGTCATTCTCTTTTACAACTTCTAAATGATGTTTAATAGAATATACCTGACAACTTCTTTGTTTAATTAACTTGTTAATGATATTACAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGTATTGAGCCTTTTCTTCTAGAACATTTGTGATTTTTCATGTACTGGAATGAGTGTTTGATTTAGAAAACCAATTTCCTGAGATGTGTAATGAAAGCTACCAAGTGTGAGATTTGAAGTGTCTGTTAATTCCAAAAATTCAAAATTCTCCCTTCGAGGACTAAGAACCAGTATCGGCAATGTTTGTACTTTAGATGGTGAAAGTGTCACCTGTATGATCATTGTTAGGGGAAGGTATTACCATAAGAATGCTCATTATGTCTGGGTGAAAGTCACAAGTATTATGATTATCAATTTTTTTTTACTAGTAAGATAATGGATGCAATTATTTGTTGGCATCATTTATTCCTATTTTTCTGATGACAAGTCTGAATATTGTTGCAGCTGTCTTTTGAACTGTGGCTTTGATCTTGCATATTCAATAAAAAAATTTATTTATTTCTTGCAGTTTGGTTTTTATTAAACGTTCAATGCTATTATCTCACTTTTAATTATATTGAAATGTTGTTGGTCTCTACCTGCTACAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTAAGCTTTCTGACTTTGATAGTTCATGCTCAGTTCAGGTTGCAAATAATATTATTCTTACCATCATCTGAGCTTTGCTTTTGCCTGTACCTATGTAGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTAAGAATATTTCATTTTGTGCTCCATATCTGTTGCAAATTATTGGCGGGCTCAAGAATTGACATTTAGATATTATTGAGGCTGGTATGGTGCTTTTTGTGGAAATAAAGCACGGGAGGGGCAGTTGAATTGCCGTCATAATGAATATCTAGGTTTTGCTTTCCATTGTGCAATGTGCAATGTGCAATTTGAAGTCTGCAGGTAGAGCAGTAGTTTCTACTGCAAGTTGAATTTGAATTTTGCTTTGTAAAGGAATGCTTATGAGTCTCTACCTTGCCTTTTGATTTACATGCACTAAAAAGAAAGCATAAAATATAAAAAACGTGCATGGAGGAGAGAAAAAAGAGTAAGAAAAAAAGTAATAATTATTTAGAAAAAGAAATAAATAAAATTAAAAGAAAAATTATAAAAAACGTGCATGGAGGAGAGAGAAGTATGGAGTAATTATTACTTTTTTTAAGTGAAAAATACCTTTTTAGTTCCTGAGTTTTCAAGATTAGGTGCGTTTGGTATCTGAGATTTCAATTTGGCAATTTTAGTCCCTCGGTTTTACAAAATAGGTTTAAAAGGTCCTTATTTTCATTTTTTAAATTAATTAGATATCTTTTTATATTATTTTAAATTTTGATATGACATTTTTAAATAAAAATATATTTTCTCTCTCCTCTATCCTCCCATTCACTCTCCGCTTTCCTTTTTTCTCTCTACTCCCCTCCTTCCACCATCGTTCCTAGTTCATATCTCCCTTCTCCAATGGACACATTCCCTTTGGCCTCTCCTTTTCTTTTGTCCTTCAAACCTTCTAAATTTTCATAAGATAGGCCATGTTGTCAAGTAGAGAAGGATTAAACTTCTAACTACCTTAAGTTTCATGAGAATGCTTATGCCCATATTCTTTCAACAAGCCATAGATCGTTTCGAACTTTCAACTATCAGATTCGTACTCTGTAATTTAAAATTGAGGAGAGCTCGATATAAATGGAGAGTATGAAATTGTAGAAACTCTTTGAAATGTTCATATAAAGATTTGCGGATTTGATTTTTTTTTCTGTCCACGCCAATGGTATAGAATCAAAAACAAGTTCTCGCCAAGTGGGTGCTGTTGAATTCAATCGTGGTCCGCTAGATCTCTAAAATCCCTTCCTTCTAGATCGACTTCTTGACATAATCAGATCTGCTATTGAAGAAAATGTATTAGTAAAGAGGGAATAATAGTGAAGGGAGAGGAAGAGGGAGAAAAAGAGGAAGAGAGAATAATCGTGGAGAGGGGAGGAGAGAGAAGAGTGAGAAATGAGGGTGAATGGGAAGAGAGGAGAGAGAAAGTATATATTTTTTTATTTAAAAATGTCATATCAAAATTTAAAATAATATAGAAAATATCTAAATTATTTAGAAAATGAGAATATAGACCTTTTAAAATTATTTTGTAAAATTGGAAGACAAATGGCTAAATTGAAAATTTAGGGACCAAATGCATCCATTCTCAAAAACTGAGGGACTACAAAAGTATTTTTCCTTTTTTAAAAAGAGTAATTATTCTTCGAAGAAGGAAAACCACTTTATGTGAATCAAGAAAGTTACATGTATATTGAGAAAGTTACATGTATATTGAGGTTCATTATCTTTAAATTTCCCAACAATAATTATTCAAAACAATTTTATTTAAAACATCGGTCAGGGGTGGTCGGAATGGCGCTTTCCAACCACTCCACTTGCGGACCTTTGGTTTTCTATGTACAAAAAACCGACCAGGACCGAATTTTTTACAAAACCGGCGGGCCAATGTCAGTTTGGCCGGTTTGGTTTGCTCACCCCGTGTAGAAGGATAACTCTTTTTTTTTTTTTTTTGCTCGATGCCATTTTCCCTACTTCTGATAAATAATCTATTACACCTTGACGTCGTGTAACTTTAACACAAAAATCCTTTCCGGACCATTGCATCTTATCACTTACTTTAAATTCTAATTATTTGTAATTGTGTTCTCAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAAGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAACACAATGTCACCTAA

mRNA sequence

ATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAAATAGGAAAAAGAAAATGAAAAGAACACGCAGACGTGGACAACTATCTGATAGAAATGAAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAAGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAACACAATGTCACCTAA

Coding sequence (CDS)

ATGGGATCTGGCAACGAAGATTCCGAAGACGAAGCCGGAGTCTCTGCCATTAGGGCTCTCGGCTCTCTCTTTAAGTTGACCGAAGTCTTTCTCTGGGACGATGAGACAGAATTAACTAGACGAGTGGAAAGTACTGGTCAGTTGGATTTTTTTTGGCCAGGTATCAGCTTATTACCGGAGGATATCGAACTTACTGAACAGATGAATGCTTTGGGGCTTCCTCTTTCGTTCCACACAAACAAAGAGAAGAGAATTGGAATCACTATGGGTAAAAGAAAAGCAACTGTCAAGCATTTTAGAACCCAGGAAGGACTTCTAGACAAAGAATTGGAGCTTCCCAATGCCAGCAGCAGGGGGGAGATTGAAGCTAACATTAATTTCAATGATGATGCTATAGGTTCTCTATCATACTCGTCTATGGTGAACCAAAGTGAGACATCTGATCATGATGTTGTGTTGGATGCCAATGAATCCCATGTCATCTTTGATCAAAATATTTCACCTAATTCAAGTGGACCTATAAGTGGTGCTGTTGAAGAACAATCTTGCGATGTTATGTGCAATTTTGTGCTAAATAATGGGGGAGACCATGAATTAAGCTTAGGTGATGCTGTGCTGGGGGATCATAGTCATACAAAAGTTAGATTGAGTTCAATTGGTTTAGATAAAGTTCATTCTTCAAGAATGTGTATGACAGGTCTTGACGTCAGCGATAGCAAGCAGGAGGAAGCTGAACCACCTATGGAGTCAGAAGGTTCGTCCACAACTTTACAAGATACTGAAGTGCAGATGACCAACATTGATAGTGCCATTGGACTGCCAGTAGTAGCTGAATCATCTTTCCTTCATACAGAAGCGGACTATAATGAAGATGACCATGTTGTTGGATGTCCTCATGGATCTGGAGAATGGATGGTGTATTGGGATTCTTTTTATATGAGAAACTACTTCTATAATATGAAGACACATGAGTCTACTTGGAATCCTCCTCCAGGGTTGGAACATTTTGCACTTTCTGATGCCAATTTCACAGAAAATAAACCAATTTCTGAAGTTGTTGAAATGGATGTATTAGAAGACGTAAAATCAGAAGATATTTGTAGTGTGCTTGGTGAAACCAGGTCATGTATGAATTTACTTGGTGATAATGTGCATTGTCAACCACCTGATGCACTGTTGGAGGGCTCTGAGAGTAGTGCATCTGTCAATACTTCAGTTAACAGTTATAAGCAATCTGACGAACCTCAAGAGTGGCAGATGAGTTGTAAAAATATTGGGGAAAATATCAGATGCAGTTGTGAAGGTCATGTCAAACAACTATGTCATGATAACTGCAGCAATGGTTTCCAGCTCATTGTTGCAAATGGAGCTTCTGAACAAAAGTCCTTTGTCCATCGCAAACCTAGTAACATGGACTCACCTGAGATAGGTTGTGTCACCACAGATGATGATGAAGATGCAGTGGGTTTAGCTACTAACAGTGCTTCTCATATGCTTCAGCAGGCAGATCATATGGATGGTGATATGCATTTTGGAAATGGACCTACCATATGTACACTGGGTACTGAGCAAAATCTTTCTGGAAGAAATAGGAAAAAGAAAATGAAAAGAACACGCAGACGTGGACAACTATCTGATAGAAATGAAGAATTTCATTCTCCCACAATCACTGAAGAGTATCCCACAAGTATTACTAAGTATTGGTGTCAGAGGTATCGACTTTTCTCCAGATTTGATGATGGTGTAAAAATGGACAAAGAAGGATGGTTTTCTGTAACTCCAGAGCCTATAGCTAGGCATCACGCATCACGTTGTGGTAGCAATATGATAATTGACTCTTTCACTGGTGTTGGTGGAAATGCCATCCAGTTTTCCCAAAGGGCCAAACATGTTATCGCAATTGATATTGATCCAACAAAGATTAGATATGCCCAACATAATGCAGCCATATATGGTGTTGAAGATCAAATAGATTTCATAAAGGGGGACTTCTTCCGTTTGGCCCCACATCTGAAGGCCGACGTTATATTCTTATCACCTCCTTGGGGAGGCCCGGATTATGCCAGAGTAGATATTTATGACCTACAGACCATGCTCAAACCACATGATGGGTATTTTCTCTTCAACATTGCTAAGAAAATTGCTCCCGTCGTTGTCATGTTTCTTCCAAAGAATGTTAATCTTGACCAACTAGCGGAGCTGTCTCTTTCTTCAGATCCTCCGTGGTCACTTGAGGTTGAGAAAAACTTTTTAAATGGCAAGCTGAAAGCGATTACTGCTTACTTTAGCAATGGCTCTATGAACGAACACAATGTCACCTAA

Protein sequence

MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLDFFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLLDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHSSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTENKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALLEGSESSASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASEQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICTLGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSNGSMNEHNVT
Homology
BLAST of HG10014129 vs. NCBI nr
Match: XP_038897817.1 (uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida])

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 676/794 (85.14%), Postives = 704/794 (88.66%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MG  +E+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                  GISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKH RTQ+G L
Sbjct: 61  KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHSRTQQGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E PNASSR EI ANINFNDDAIGSL Y SMVNQSE SD DVVLDANESHVI   NI
Sbjct: 121 DKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGNI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           SPN S  ISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD  HT+VR SSIGL KVHS
Sbjct: 181 SPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD--HTEVRSSSIGLVKVHS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            RMCMTGLDV   KQEE EPPME EGSS TLQDTEVQ ++IDS IGLP+V ESS LHTEA
Sbjct: 241 PRMCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTEA 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           DYNEDDHVVGC   SGEWMVYWDSFY RNYFYNMKTHESTWNPP GLEHFA SDANFTEN
Sbjct: 301 DYNEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTEN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSESS 420
           +P + VV+MDVLED+KSEDIC VL +TRSCMNL+GDNVHCQPPDALL       EGSE  
Sbjct: 361 EPSAGVVQMDVLEDIKSEDIC-VLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSERV 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           ASVNT VNSYKQSDEPQE QMSCKNIGENI CSCEGHVKQLCH+NCSNGFQLIVAN ASE
Sbjct: 421 ASVNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVASE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           QK+F H KPSNM+SPE+ CVT DDDE AVGLAT+SASHM QQADHMDGDM+FGNGPTICT
Sbjct: 481 QKTFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGTEQNLSGRNRKKKMKRTRRRGQLS+RNE FHS  ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DGVKMDKEGWFSVTPEPIARHHASRCGSN IIDSFTGVGGNAIQFSQRAKHVIAIDIDP 
Sbjct: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDPI 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAAIYGV DQIDFIKGDFF LAPHLKADVIFLSPPWGGPDYARVDIYDL+TML
Sbjct: 661 KIRYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTML 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           KPHDGYFLFNIAKKIAPV+VMFLPKNV+LDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT
Sbjct: 721 KPHDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. NCBI nr
Match: XP_038897816.1 (uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida])

HSP 1 Score: 1326.6 bits (3432), Expect = 0.0e+00
Identity = 676/795 (85.03%), Postives = 704/795 (88.55%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MG  +E+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMGKRKATVKHFRTQEGL 120
                  GISLLPEDIELTEQMNALGLPLSFHTNKE KRIGITMGKRKATVKH RTQ+G 
Sbjct: 61  KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHSRTQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E PNASSR EI ANINFNDDAIGSL Y SMVNQSE SD DVVLDANESHVI   N
Sbjct: 121 LDKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGN 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPN S  ISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD  HT+VR SSIGL KVH
Sbjct: 181 ISPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGD--HTEVRSSSIGLVKVH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S RMCMTGLDV   KQEE EPPME EGSS TLQDTEVQ ++IDS IGLP+V ESS LHTE
Sbjct: 241 SPRMCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTE 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNEDDHVVGC   SGEWMVYWDSFY RNYFYNMKTHESTWNPP GLEHFA SDANFTE
Sbjct: 301 ADYNEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+P + VV+MDVLED+KSEDIC VL +TRSCMNL+GDNVHCQPPDALL       EGSE 
Sbjct: 361 NEPSAGVVQMDVLEDIKSEDIC-VLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSER 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNT VNSYKQSDEPQE QMSCKNIGENI CSCEGHVKQLCH+NCSNGFQLIVAN AS
Sbjct: 421 VASVNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM+SPE+ CVT DDDE AVGLAT+SASHM QQADHMDGDM+FGNGPTIC
Sbjct: 481 EQKTFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTIC 540

Query: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TLGTEQNLSGRNRKKKMKRTRRRGQLS+RNE FHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPEPIARHHASRCGSN IIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAAIYGV DQIDFIKGDFF LAPHLKADVIFLSPPWGGPDYARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 774
           LKPHDGYFLFNIAKKIAPV+VMFLPKNV+LDQLAELSLSSDPPWSLEVEKNFLNGKLKAI
Sbjct: 721 LKPHDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 780

BLAST of HG10014129 vs. NCBI nr
Match: XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 641/794 (80.73%), Postives = 680/794 (85.64%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKE+R GITMGKRK TVKH R Q+G L
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQQGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +I
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           SPNSSG I GAVEEQ CDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK HS
Sbjct: 181 SPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGHS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  A
Sbjct: 241 PRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGA 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           DYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DANFTEN
Sbjct: 301 DYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTEN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSESS 420
           + I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S 
Sbjct: 361 ESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSR 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           ASVNTS++SY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN ASE
Sbjct: 421 ASVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           QK+F H KPSNM +PE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTICT
Sbjct: 481 QKTFSHCKPSNMYAPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGTEQNLSGR+RKKKMKRTRRRGQLSD+NEEFHSP ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT
Sbjct: 601 DGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TML
Sbjct: 661 KIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTML 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           +PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAIT
Sbjct: 721 RPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. NCBI nr
Match: XP_023535334.1 (uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535335.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535336.1 uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1255.4 bits (3247), Expect = 0.0e+00
Identity = 641/795 (80.63%), Postives = 680/795 (85.53%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKE +R GITMGKRK TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQ CDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS++SY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM +PE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYAPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TLGTEQNLSGR+RKKKMKRTRRRGQLSD+NEEFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 774
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of HG10014129 vs. NCBI nr
Match: XP_022975956.1 (uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 635/794 (79.97%), Postives = 676/794 (85.14%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKE+R GITMGKRK TVKH R Q G L
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +I
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           SPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK HS
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGHS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  A
Sbjct: 241 PRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGA 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           DYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTEN
Sbjct: 301 DYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTEN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSESS 420
           + I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S 
Sbjct: 361 ESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSR 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  SE
Sbjct: 421 ASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           QK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTICT
Sbjct: 481 QKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGTEQNLSGR+RKKKMKRTRRRGQLSDRNEEFHS  ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 
Sbjct: 601 DGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPI 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TML
Sbjct: 661 KIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTML 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           +PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAIT
Sbjct: 721 RPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. ExPASy Swiss-Prot
Match: P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.7e-66
Identity = 135/292 (46.23%), Postives = 186/292 (63.70%), Query Frame = 0

Query: 477 GCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICTLGTEQNLSGRNRKKKMK 536
           G + T D E       +SAS +  +A+  +G       P  C+     N      + ++K
Sbjct: 554 GLMETRDPEPENCQTISSASEL--EAEKSEGGSLVAAVPENCSTEGVANSPRAEAEVEIK 613

Query: 537 RTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEP 596
           + +++ + +   +    P      P  + KYW QRYRLFSRFDDG+K+DKEGWFSVTPE 
Sbjct: 614 KKKKKKKKNKNKKINGLPPEIASVP-ELAKYWAQRYRLFSRFDDGIKLDKEGWFSVTPEK 673

Query: 597 IARHHASRCGS----NMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYG 656
           IA H A R       ++I+D+F GVGGN IQF+   K VIAIDIDP KI  A++NA +YG
Sbjct: 674 IAEHIAGRVSQSFNCDIIVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNAEVYG 733

Query: 657 VEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAK 716
           V D+I+FI GDF  LAP LKADV+FLSPPWGGPDYA  + +D++TM+ P DG+ +F +++
Sbjct: 734 VADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIFRLSQ 793

Query: 717 KIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 765
           KI   +V FLP+N ++DQ+A L   + P   +E+E+NFLN KLK ITAYF +
Sbjct: 794 KITNNIVYFLPRNADVDQVASL---AGPGGQVEIEQNFLNNKLKTITAYFGD 838

BLAST of HG10014129 vs. ExPASy Swiss-Prot
Match: Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)

HSP 1 Score: 250.8 bits (639), Expect = 5.2e-65
Identity = 116/205 (56.59%), Postives = 152/205 (74.15%), Query Frame = 0

Query: 564 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGS----NMIIDSFTGVGG 623
           + KYW QRYRLFSRFDDG+K+D+EGWFSVTPE IA H A R       ++++D+F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703

Query: 624 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLS 683
           N IQF+     VIAIDIDP KI  A++NA +YG+ D+I+FI GDF  LA  LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763

Query: 684 PPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSD 743
           PPWGGPDYA  + +D++TM+ P DG+ +F ++KKI   +V FLP+N ++DQ+A L   + 
Sbjct: 764 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSKKITNNIVYFLPRNADIDQVASL---AG 823

Query: 744 PPWSLEVEKNFLNGKLKAITAYFSN 765
           P   +E+E+NFLN KLK ITAYF +
Sbjct: 824 PGGQVEIEQNFLNNKLKTITAYFGD 844

BLAST of HG10014129 vs. ExPASy Swiss-Prot
Match: Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)

HSP 1 Score: 250.0 bits (637), Expect = 8.8e-65
Identity = 123/236 (52.12%), Postives = 164/236 (69.49%), Query Frame = 0

Query: 533 KKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSV 592
           KK K+  +  +++D   E  S          + KYW QRYRLFSRFDDG+K+DKEGWFSV
Sbjct: 612 KKKKKKNKNKKINDLPPEIAS-------VPELAKYWAQRYRLFSRFDDGIKLDKEGWFSV 671

Query: 593 TPEPIARHHASRCGS----NMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNA 652
           TPE IA H A R       ++++D+F GVGGN IQF+   K VIAIDIDP KI  A++NA
Sbjct: 672 TPEKIAEHIAGRVSQAFRCDVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARNNA 731

Query: 653 AIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLF 712
            +YG+ D+I+FI GDF  LAP LKADV+FLSPPWGGPDYA  + +D++TM+ P DG+ +F
Sbjct: 732 EVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFEIF 791

Query: 713 NIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 765
            +++KI   +V FLP+N ++DQ+A L+        +E+E+NFLN KLK ITAYF +
Sbjct: 792 RLSQKITNNIVYFLPRNADIDQVASLAGLGG---QVEIEQNFLNNKLKTITAYFGD 836

BLAST of HG10014129 vs. ExPASy Swiss-Prot
Match: Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)

HSP 1 Score: 157.1 bits (396), Expect = 7.8e-37
Identity = 90/229 (39.30%), Postives = 127/229 (55.46%), Query Frame = 0

Query: 546 DRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPE----PIARHH 605
           D +E      I    P ++ KYW  RY LFSRFD+G+ +D + W+SVTPE     IA+  
Sbjct: 8   DEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVAIAKSV 67

Query: 606 ASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGV-EDQIDFI 665
                  +IID+F+G GGN IQF++    VI+I+IDP KI  A+HN  IYG+   ++ FI
Sbjct: 68  VDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSSRVTFI 127

Query: 666 KGDFFRLAPHLK-----ADVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIA 725
           +GD       L+       ++F+SPPWGGP Y+   +Y L   L P+    LF  A +I+
Sbjct: 128 QGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKEATRIS 187

Query: 726 PVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFL-NGKLKAITAYFS 764
           P V  FLP+N ++ +LA      + P+      NFL  G  KAI  YF+
Sbjct: 188 PYVAAFLPRNTDVKELAAYGSIHNKPYI----TNFLFEGYAKAICCYFN 230

BLAST of HG10014129 vs. ExPASy Swiss-Prot
Match: Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.7e-29
Identity = 74/178 (41.57%), Postives = 100/178 (56.18%), Query Frame = 0

Query: 566 KYWCQRYRLFSRFDD-GVKMDKEGWFSVTPEPIA---RHHASRCGSN--MIIDSFTGVGG 625
           KYW  R RLFS+ D   + M  E WFSVTPE IA    +    C  N   I+D F G GG
Sbjct: 51  KYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGG 110

Query: 626 NAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLA-----PHLKAD 685
           N IQF+ +  +V  +D     I     NA  YGV+D+I   +G + +L        +K D
Sbjct: 111 NTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYD 170

Query: 686 VIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLA 733
            +F SPPWGGP+Y R D+YDL+  LKP     +     K++P V+MFLP+N +L+QL+
Sbjct: 171 CVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLS 228

BLAST of HG10014129 vs. ExPASy TrEMBL
Match: A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 635/794 (79.97%), Postives = 676/794 (85.14%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKE+R GITMGKRK TVKH R Q G L
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +I
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           SPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK HS
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGHS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  A
Sbjct: 241 PRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGA 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           DYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTEN
Sbjct: 301 DYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTEN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSESS 420
           + I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S 
Sbjct: 361 ESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSR 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  SE
Sbjct: 421 ASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           QK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTICT
Sbjct: 481 QKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGTEQNLSGR+RKKKMKRTRRRGQLSDRNEEFHS  ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 
Sbjct: 601 DGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPI 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TML
Sbjct: 661 KIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTML 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           +PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAIT
Sbjct: 721 RPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. ExPASy TrEMBL
Match: A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 635/795 (79.87%), Postives = 676/795 (85.03%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDEAGVSAIRA+GSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDI+LTEQMNALGLPLSFHTNKE +R GITMGKRK TVKH R Q G 
Sbjct: 61  KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P  SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GAVEEQSC+V C+ VLNN GDHE   GDA+LGDH+  KVRLS IGLDK H
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHA--KVRLSPIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE S+LH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPPPGLEHFA SDANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS+S
Sbjct: 361 NESIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKS 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASV+TS+NSY Q DEP EW  SC+N  E I CSCEGHVKQ CH+NCSNGFQLIVAN  S
Sbjct: 421 RASVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H K SNMDSPE   VT  DDE AVGL T+S SH+LQQADHMDGDMHFGN PTIC
Sbjct: 481 EQKTFSHCKSSNMDSPEKAFVTI-DDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTIC 540

Query: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TLGTEQNLSGR+RKKKMKRTRRRGQLSDRNEEFHS  ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
            KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 IKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 774
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of HG10014129 vs. ExPASy TrEMBL
Match: A0A6J1FDE5 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 634/794 (79.85%), Postives = 676/794 (85.14%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGSGNE+S  EAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKE+R GITMGKR  TVKH R Q+G L
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E P +SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +I
Sbjct: 121 DKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           SPNSSG I GA EEQSCDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK HS
Sbjct: 181 SPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGHS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  A
Sbjct: 241 PRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGA 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           DYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DANFTEN
Sbjct: 301 DYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTEN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSESS 420
           + I+EV EMDVLED+K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS++ 
Sbjct: 361 ESIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNR 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           ASVNTS+NSY Q DEP EW  + +N  E I CSCEGHVKQ CH+NCSNGFQLIVAN ASE
Sbjct: 421 ASVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           QK+F H KPSNM SPE   VT  DDE AV L T+S SH+LQQADHM+GDMHFGN PTICT
Sbjct: 481 QKTFSHCKPSNMYSPEKAFVTI-DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGTEQNLSGR+RKKKMKRTRRRGQ SDRNEEFHSP ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DGVKMDKEGWFSVTPE IARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDPT
Sbjct: 601 DGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TML
Sbjct: 661 KIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTML 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           +PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAIT
Sbjct: 721 RPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. ExPASy TrEMBL
Match: A0A6J1FD09 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 634/795 (79.75%), Postives = 676/795 (85.03%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGSGNE+S  EAGVSAIRALGSLFKLTEVFLWDDETE+ RRVES+  LD           
Sbjct: 1   MGSGNEES--EAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKE-KRIGITMGKRKATVKHFRTQEGL 120
                   ISL PEDIELTEQMNALGLPLSFHTNKE +R GITMGKR  TVKH R Q+G 
Sbjct: 61  KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKEQRRTGITMGKRNTTVKHSRIQQGF 120

Query: 121 LDKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQN 180
           LDKE+E P +SSRGEI ANIN ND+AIGSL  SSMVNQSE SD D V +ANESHVIFD +
Sbjct: 121 LDKEVEFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180

Query: 181 ISPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVH 240
           ISPNSSG I GA EEQSCDV C+ VLNN GDHE   GDAVLGDH+  KVRLSSIGLDK H
Sbjct: 181 ISPNSSGLIHGAFEEQSCDVTCDIVLNNRGDHE--SGDAVLGDHA--KVRLSSIGLDKGH 240

Query: 241 SSRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTE 300
           S R+CMTG DVS  KQEE E PME EGSSTTLQDTEVQ  +IDS IGLP+VAE SFLH  
Sbjct: 241 SPRICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMG 300

Query: 301 ADYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTE 360
           ADYNE+DHVVGC    GEW VYWDSFYMRNYFYN+KTHESTWNPP GLEHFA  DANFTE
Sbjct: 301 ADYNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTE 360

Query: 361 NKPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALL-------EGSES 420
           N+ I+EV EMDVLED+K EDICSVL +TRSCMNL GDN+HCQPPDALL       EGS++
Sbjct: 361 NESIAEVAEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKN 420

Query: 421 SASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGAS 480
            ASVNTS+NSY Q DEP EW  + +N  E I CSCEGHVKQ CH+NCSNGFQLIVAN AS
Sbjct: 421 RASVNTSINSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEAS 480

Query: 481 EQKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTIC 540
           EQK+F H KPSNM SPE   VT  DDE AV L T+S SH+LQQADHM+GDMHFGN PTIC
Sbjct: 481 EQKTFSHCKPSNMYSPEKAFVTI-DDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTIC 540

Query: 541 TLGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRF 600
           TLGTEQNLSGR+RKKKMKRTRRRGQ SDRNEEFHSP ITEEYPTSITKYWCQRY+LFSRF
Sbjct: 541 TLGTEQNLSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRF 600

Query: 601 DDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
           DDGVKMDKEGWFSVTPE IARHHASRCGSNMI+DSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDKEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660

Query: 661 TKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTM 720
           TKIRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YARVDIYDL+TM
Sbjct: 661 TKIRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTM 720

Query: 721 LKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 774
           L+PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE++LSS+PPWSLEVEKNFLNGKLKAI
Sbjct: 721 LRPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLKAI 780

BLAST of HG10014129 vs. ExPASy TrEMBL
Match: A0A0A0L4V8 (Trimethylguanosine synthase OS=Cucumis sativus OX=3659 GN=Csa_4G639840 PE=4 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 623/794 (78.46%), Postives = 661/794 (83.25%), Query Frame = 0

Query: 1   MGSGNEDSEDEAGVSAIRALGSLFKLTEVFLWDDETELTRRVESTGQLD----------- 60
           MGS NE+SEDE GVS IRALGSLFKLTEVFLWD+ETE+ RRVES   LD           
Sbjct: 1   MGSCNEESEDEPGVSPIRALGSLFKLTEVFLWDEETEVARRVESRLALDADDANNGKSVE 60

Query: 61  ---FFWPGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHFRTQEGLL 120
                  GISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITM KRKA VKH R Q+G L
Sbjct: 61  KICSTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMVKRKANVKHSRIQQGFL 120

Query: 121 DKELELPNASSRGEIEANINFNDDAIGSLSYSSMVNQSETSDHDVVLDANESHVIFDQNI 180
           DKE+E P ASSR EI AN  FNDDA GSL   SMVNQSETSD DVVLD NE HVIFD +I
Sbjct: 121 DKEVEFPKASSREEIVANSTFNDDATGSLCSYSMVNQSETSDRDVVLDTNEIHVIFDGDI 180

Query: 181 SPNSSGPISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHSHTKVRLSSIGLDKVHS 240
           S NSSG ISGAVEEQ CDVMC+ VLNNGGDHELS  DAVLGD  HTKVRLSSIG DK +S
Sbjct: 181 SRNSSGVISGAVEEQFCDVMCDIVLNNGGDHELSSDDAVLGD--HTKVRLSSIGFDKGYS 240

Query: 241 SRMCMTGLDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEA 300
            R+  TGLDV   KQEE EPPMESEGSSTT QDTEVQ ++ DS I LP VAE  FL  E 
Sbjct: 241 PRLRTTGLDVGHGKQEEVEPPMESEGSSTTFQDTEVQKSDTDSGIVLPEVAEPCFLRMEP 300

Query: 301 DYNEDDHVVGCPHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTEN 360
           D NE+D VVGC H SG+WMVYWDSFYMRNYFYN+K+HESTWNPP GLEHFA SDANFT N
Sbjct: 301 DCNENDQVVGCIHESGDWMVYWDSFYMRNYFYNIKSHESTWNPPLGLEHFASSDANFTPN 360

Query: 361 KPISEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDALLEGS-------ESS 420
           +  +EV EMDVLEDVKSEDIC VLG+T  CMNLLGD+VHCQPPDALLEGS       ESS
Sbjct: 361 ESTAEVCEMDVLEDVKSEDICRVLGDT-ECMNLLGDSVHCQPPDALLEGSSSLIEGIESS 420

Query: 421 ASVNTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASE 480
           A ++TS+N  K  DEPQEW MSC+N  ENI CSCEGH KQ C +NC+NG Q I ANGASE
Sbjct: 421 AFIDTSINCSK--DEPQEWLMSCRNTRENIGCSCEGHAKQSCGENCTNGSQFIAANGASE 480

Query: 481 QKSFVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICT 540
           Q  F H KPSNM SPEI C+T DDDE   GL T+S SHMLQQADH+DGDMHF NGP ICT
Sbjct: 481 QMMFSHHKPSNMHSPEIDCITIDDDEGTAGLTTSSVSHMLQQADHIDGDMHFANGPIICT 540

Query: 541 LGTEQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFD 600
           LGT QNLS RNRK+KMKRTRRRGQLSDRNE F S  ITEEYPTSITKYWCQRY+LFSRFD
Sbjct: 541 LGTVQNLSVRNRKRKMKRTRRRGQLSDRNEGFRSFAITEEYPTSITKYWCQRYQLFSRFD 600

Query: 601 DGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPT 660
           DG+KMDKEGWFSVTPEPIARHHASRCGSNMIID FTGVGGNAIQFSQRAKHVIAIDIDPT
Sbjct: 601 DGIKMDKEGWFSVTPEPIARHHASRCGSNMIIDGFTGVGGNAIQFSQRAKHVIAIDIDPT 660

Query: 661 KIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTML 720
           KIRYAQHNAAIYGVEDQIDF+KGDFFRLAPHLKADVIFLSPPWGGPDYA VDIYDL T L
Sbjct: 661 KIRYAQHNAAIYGVEDQIDFLKGDFFRLAPHLKADVIFLSPPWGGPDYAGVDIYDL-TKL 720

Query: 721 KPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 774
           KPHDGYFLFN+AKKIAP+VVMFLPKNVNL+QLAELSLSSDPPWSLEVEKNFLNGKLKAIT
Sbjct: 721 KPHDGYFLFNVAKKIAPLVVMFLPKNVNLNQLAELSLSSDPPWSLEVEKNFLNGKLKAIT 780

BLAST of HG10014129 vs. TAIR 10
Match: AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 5.4e-94
Identity = 213/540 (39.44%), Postives = 286/540 (52.96%), Query Frame = 0

Query: 234 LDVSDSKQEEAEPPMESEGSSTTLQDTEVQMTNIDSAIGLPVVAESSFLHTEADYNEDDH 293
           L++  +  EE E P   E     +Q  EV+  N +  +G      S FL       + D 
Sbjct: 108 LNLVSALNEEVESPCFEE---DCVQVIEVEEENHEVVVG------SCFLGN----GDGDS 167

Query: 294 VVGC----PHGSGEWMVYWDSFYMRNYFYNMKTHESTWNPPPGLEHFALSDANFTENKPI 353
           V+       H S  W VYWDSFY R+YFYN KT ES W PP G+EH A SD    E+  +
Sbjct: 168 VLASETIDSHDSSVWKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSD----ESHNL 227

Query: 354 SEVVEMDVLEDVKSEDICSVLGETRSCMNLLGDNVHCQPPDAL-------LEGSESSASV 413
           SE+V     +D+                 +LGD+V     D L       LE +E++  V
Sbjct: 228 SELVIEKHHDDLSG--------------TVLGDDVPFDKADDLGGVCQSQLE-AEATEEV 287

Query: 414 NTSVNSYKQSDEPQEWQMSCKNIGENIRCSCEGHVKQLCHDNCSNGFQLIVANGASEQKS 473
           N+ +++Y+++           +IG                                    
Sbjct: 288 NSLIDTYQET-----------SIGN----------------------------------- 347

Query: 474 FVHRKPSNMDSPEIGCVTTDDDEDAVGLATNSASHMLQQADHMDGDMHFGNGPTICTLGT 533
                  ++D   +G       E+  G    S                            
Sbjct: 348 ------QSLDITSLG-------EEGTGAYVVS---------------------------- 407

Query: 534 EQNLSGRNRKKKMKRTRRRGQLSDRNEEFHSPTITEEYPTSITKYWCQRYRLFSRFDDGV 593
               S R  KK+ +R+R + +L +         + EEY   + KYWCQRY LFSRFD+G+
Sbjct: 408 ----SVRKAKKESRRSRAKKKLLNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDEGI 467

Query: 594 KMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIR 653
           KMD+EGWFSVTPE IA+HHA+RC   ++ID FTGVGGNAIQF+ R+ +VIAID+DP K+ 
Sbjct: 468 KMDEEGWFSVTPELIAKHHATRCNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKKLD 524

Query: 654 YAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWGGPDYARVDIYDLQTMLKPH 713
            A+HNAAIYGV D+IDF+KGDFF LA +LKA  +FLSPPWGGPDY +   YD++TML+P 
Sbjct: 528 LAKHNAAIYGVADKIDFVKGDFFDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLRPR 524

Query: 714 DGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWSLEVEKNFLNGKLKAITAYF 763
           DG  LF  A  IA  ++MFLP+NV+++QLAEL+LS+ PPWSLEVEKN+LNGKLKA+TAY+
Sbjct: 588 DGDALFKAAMNIASTIIMFLPRNVDINQLAELALSTSPPWSLEVEKNYLNGKLKAVTAYY 524

BLAST of HG10014129 vs. TAIR 10
Match: AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 2.3e-68
Identity = 120/211 (56.87%), Postives = 158/211 (74.88%), Query Frame = 0

Query: 556 ITEEYPTS--ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDS 615
           I +E+ T+  I++YW QRY LFS++D G++MD+EGW+SVTPE IA   A RC   ++ID 
Sbjct: 9   IEKEHGTNPKISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDC 68

Query: 616 FTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKA 675
           F+GVGGN IQF++    VIAIDIDP KI  A +NA +YGV ++IDF+ GDF +LAP LK 
Sbjct: 69  FSGVGGNTIQFAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKG 128

Query: 676 DVIFLSPPWGGPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAE 735
           DV+FLSPPWGGP Y++V+ Y L  ML P DGY LF  A  I P ++MFLPKN++L QL E
Sbjct: 129 DVLFLSPPWGGPTYSKVESYKLD-MLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEE 188

Query: 736 LSLSSDPPWSLEVEKNFLNGKLKAITAYFSN 765
           L+  S PP +LE+E+N + G++KAITAYFS+
Sbjct: 189 LACLSSPPLTLEIEENSIGGEIKAITAYFSS 218

BLAST of HG10014129 vs. TAIR 10
Match: AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 6.7e-68
Identity = 115/200 (57.50%), Postives = 152/200 (76.00%), Query Frame = 0

Query: 564 ITKYWCQRYRLFSRFDDGVKMDKEGWFSVTPEPIARHHASRCGSNMIIDSFTGVGGNAIQ 623
           ITKYW QRY LFSR+D G++MD+EGW+SVTPE IA   A R    ++ID F+GVGGN IQ
Sbjct: 253 ITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCFSGVGGNTIQ 312

Query: 624 FSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPHLKADVIFLSPPWG 683
           F++    V+AIDIDP K+  A +NA +YGV +++DF+ GDF +LAP LK DV+FLSPPWG
Sbjct: 313 FAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGDVVFLSPPWG 372

Query: 684 GPDYARVDIYDLQTMLKPHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAELSLSSDPPWS 743
           GP Y   + Y+L  ML+P DGY LF IA+ I P ++MFLP+NV+L Q+ EL+  S PP +
Sbjct: 373 GPMYRDFESYNLD-MLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEELAWLSSPPLN 432

Query: 744 LEVEKNFLNGKLKAITAYFS 764
           LE+E+NF+ G++KA+TAYFS
Sbjct: 433 LEIEENFVGGRMKAVTAYFS 451

BLAST of HG10014129 vs. TAIR 10
Match: AT3G21300.1 (RNA methyltransferase family protein )

HSP 1 Score: 48.9 bits (115), Expect = 2.1e-05
Identity = 26/80 (32.50%), Postives = 41/80 (51.25%), Query Frame = 0

Query: 606 GSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQHNAAIYGVEDQIDFIKGDFF 665
           GS +++D F G G   +  ++RAKHV   ++ P  I  A  NA I G+E+   FI+GD  
Sbjct: 399 GSEVVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGIEN-ATFIQGDLN 458

Query: 666 RLAPHL-----KADVIFLSP 681
           ++         K D++   P
Sbjct: 459 KIGEDFGNNFPKPDIVISDP 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897817.10.0e+0085.14uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida][more]
XP_038897816.10.0e+0085.03uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida][more]
XP_023535337.10.0e+0080.73uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023535334.10.0e+0080.63uncharacterized protein LOC111796805 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022975956.10.0e+0079.97uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P851074.7e-6646.23Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1[more]
Q96RS05.2e-6556.59Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3[more]
Q923W18.8e-6552.12Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2[more]
Q098147.8e-3739.30Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... [more]
Q120522.7e-2941.57Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A6J1IFL40.0e+0079.97Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1II650.0e+0079.87Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... [more]
A0A6J1FDE50.0e+0079.85Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
A0A6J1FD090.0e+0079.75Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... [more]
A0A0A0L4V80.0e+0078.46Trimethylguanosine synthase OS=Cucumis sativus OX=3659 GN=Csa_4G639840 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G45231.25.4e-9439.44S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.22.3e-6856.87S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G30550.16.7e-6857.50S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G21300.12.1e-0532.50RNA methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 557..769
e-value: 1.7E-81
score: 275.0
NoneNo IPR availableGENE3D2.20.70.10coord: 290..348
e-value: 1.3E-6
score: 29.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 528..550
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 236..256
NoneNo IPR availablePANTHERPTHR14741S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATEDcoord: 23..768
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 610..708
e-value: 7.02625E-10
score: 55.1286
IPR019012RNA cap guanine-N2 methyltransferasePFAMPF09445Methyltransf_15coord: 608..763
e-value: 4.8E-41
score: 140.1
IPR001202WW domainPROSITEPS01159WW_DOMAIN_1coord: 304..330
IPR001202WW domainPROSITEPS50020WW_DOMAIN_2coord: 304..332
score: 10.521601
IPR001202WW domainCDDcd00201WWcoord: 304..332
e-value: 0.00261894
score: 34.0406
IPR036020WW domain superfamilySUPERFAMILY51045WW domaincoord: 296..331
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 562..696

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10014129.1HG10014129.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009452 7-methylguanosine RNA capping
biological_process GO:0001510 RNA methylation
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0005515 protein binding