HG10014116 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014116
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	EF hand calcium-binding protein family
Location	Chr02: 7710705 .. 7710977 (+)
RNA-Seq Expression	HG10014116
Synteny	HG10014116

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGGAAAAGCAAAACTTGGCATTCGAGGGAGAAGCTAACAAGAGAACAGCTAGTTGTAATCTTTAGAAACCACGACAACGACAAGGACGGCCGCCTGAGCACCGCCGAACTCACGGAAGCCTTCCGGTATATCGGCGCCTTCCTGCCGTCGTACAGAGCTCGCCACGCTCTTTTTCATATCGATTCCGACAGAGACGGATTTATCGGCGAGAAGGATTTCGAATTACTCGTCAATTATGCCGAGCAACGAAAATACACCATCATTTGA

mRNA sequence

ATGGCGAGGAAAAGCAAAACTTGGCATTCGAGGGAGAAGCTAACAAGAGAACAGCTAGTTGTAATCTTTAGAAACCACGACAACGACAAGGACGGCCGCCTGAGCACCGCCGAACTCACGGAAGCCTTCCGGTATATCGGCGCCTTCCTGCCGTCGTACAGAGCTCGCCACGCTCTTTTTCATATCGATTCCGACAGAGACGGATTTATCGGCGAGAAGGATTTCGAATTACTCGTCAATTATGCCGAGCAACGAAAATACACCATCATTTGA

Coding sequence (CDS)

ATGGCGAGGAAAAGCAAAACTTGGCATTCGAGGGAGAAGCTAACAAGAGAACAGCTAGTTGTAATCTTTAGAAACCACGACAACGACAAGGACGGCCGCCTGAGCACCGCCGAACTCACGGAAGCCTTCCGGTATATCGGCGCCTTCCTGCCGTCGTACAGAGCTCGCCACGCTCTTTTTCATATCGATTCCGACAGAGACGGATTTATCGGCGAGAAGGATTTCGAATTACTCGTCAATTATGCCGAGCAACGAAAATACACCATCATTTGA

Protein sequence

MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDFELLVNYAEQRKYTII

Homology

[Close]

BLAST of HG10014116 vs. NCBI nr
Match: KAA0064376.1 (putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 159.1 bits (401), Expect = 1.8e-35
Identity = 74/90 (82.22%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          M+R+SKT+H R +L+REQL++IFRNHD+DKDGRLST ELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          H DSDRDGFI EKDFELLVNYAEQR+YT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of HG10014116 vs. NCBI nr
Match: KGN55185.1 (hypothetical protein Csa_012915 [Cucumis sativus])

HSP 1 Score: 156.0 bits (393), Expect = 1.5e-34
Identity = 74/90 (82.22%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK ++ R KL+REQL++IFRNHD+DKDGRLST ELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          H DSDRDGFIGEKDFE LVNYAEQR+YTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of HG10014116 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 150.6 bits (379), Expect = 6.5e-33
Identity = 74/90 (82.22%), Postives = 78/90 (86.67%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          MARKSKT+  R KL+REQL VIFRNHD D DGRLST ELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          H DSD DGFIGEKDFELLVNYAE+RKYT+I
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYTVI 90

[Close]

BLAST of HG10014116 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 147.9 bits (372), Expect = 4.2e-32
Identity = 73/88 (82.95%), Postives = 76/88 (86.36%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          MARKSKT+  R KL+REQL VIFRNHD D DGRLST ELT AFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYT 89
          H DSD DGFIGEKDFELLVNYAE+RKYT
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYT 88

[Close]

BLAST of HG10014116 vs. NCBI nr
Match: KAG6608481.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1 putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-29
Identity = 69/90 (76.67%), Postives = 74/90 (82.22%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          MARK+K    R KL+REQL VIFRNHD D DGRLS  ELTEAFRYIGAFLPSYRA HAL 
Sbjct: 1  MARKNKMSQPRPKLSREQLSVIFRNHDGDGDGRLSATELTEAFRYIGAFLPSYRAHHALR 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          + DSDRDGFIGEKDFE LVNY E+R YTI+
Sbjct: 61 YTDSDRDGFIGEKDFESLVNYTEKRHYTIV 90

[Close]

BLAST of HG10014116 vs. ExPASy Swiss-Prot
Match: Q39419 (Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-05
Identity = 26/67 (38.81%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 9  HSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDG 68
          H ++K  RE+   IF+  D + DG++S AEL EA + +G+  P    +H +  ID+D DG
Sbjct: 5  HPQDKAERER---IFKRFDANGDGKISAAELGEALKTLGSITPD-EVKHMMAEIDTDGDG 64

Query: 69 FIGEKDF 76
          FI  ++F
Sbjct: 65 FISFQEF 67

[Close]

BLAST of HG10014116 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 3.5e-05
Identity = 21/61 (34.43%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 15  TREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKD 74
           ++E+L  +F + D+D DG++S  EL   F  +G ++    A+ A+  +D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

[Close]

BLAST of HG10014116 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-04
Identity = 22/58 (37.93%), Postives = 33/58 (56.90%), Query Frame = 0


Query: 18 QLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDF 76
          +L  +FR +D + DG++S  EL    R +GA L     R  +  +DSDRDGF+   +F
Sbjct: 5  ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

[Close]

BLAST of HG10014116 vs. ExPASy Swiss-Prot
Match: Q8VWY6 (Polcalcin Nic t 1 OS=Nicotiana tabacum OX=4097 GN=Nict1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.9e-04
Identity = 21/55 (38.18%), Postives = 36/55 (65.45%), Query Frame = 0


Query: 22 IFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDFE 77
          IF+  D + DG++S+AEL E  + +G+ + S   +H +  +D+D DGFI  ++FE
Sbjct: 14 IFKRFDLNGDGKISSAELGETLKMLGS-VTSEEVQHMMAELDTDGDGFISYEEFE 67

[Close]

BLAST of HG10014116 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.8e-36
Identity = 74/90 (82.22%), Postives = 84/90 (93.33%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          M+R+SKT+H R +L+REQL++IFRNHD+DKDGRLST ELTEAFRYIGAFLP YRARHALF
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          H DSDRDGFI EKDFELLVNYAEQR+YT+I
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

[Close]

BLAST of HG10014116 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.5e-35
Identity = 74/90 (82.22%), Postives = 83/90 (92.22%), Query Frame = 0


Query: 1  MARKSKTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALF 60
          M+RKSK ++ R KL+REQL++IFRNHD+DKDGRLST ELTEAFRYIGAFLP+YRARHAL 
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HIDSDRDGFIGEKDFELLVNYAEQRKYTII 91
          H DSDRDGFIGEKDFE LVNYAEQR+YTII
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

[Close]

BLAST of HG10014116 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-12
Identity = 40/84 (47.62%), Postives = 56/84 (66.67%), Query Frame = 0


Query: 6  KTWHSREKLTREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSD 65
          KT  S+  L  +QL  IF+ HD + DG+L+ AEL +AF+++GA +P +RA  AL H D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLVNYAEQRKYTI 90
           DG I EK+ + LV YA +  YTI
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYTI 90

[Close]

BLAST of HG10014116 vs. ExPASy TrEMBL
Match: A0A5N6RQV2 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-12
Identity = 40/75 (53.33%), Postives = 54/75 (72.00%), Query Frame = 0


Query: 15 TREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKD 74
          TR+QL+ IFR+HD D DG+LS  E+  AF Y+G+   SYRA  AL H DSD DG++GE++
Sbjct: 16 TRDQLMNIFRSHDLDGDGKLSRKEVKAAFGYLGSRWDSYRAVLALLHADSDHDGYVGEEE 75

Query: 75 FELLVNYAEQRKYTI 90
           + LV+YA +  YTI
Sbjct: 76 LKKLVDYALKCGYTI 90

[Close]

BLAST of HG10014116 vs. ExPASy TrEMBL
Match: W9RZZ8 (Putative calcium-binding protein CML27 OS=Morus notabilis OX=981085 GN=L484_006332 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 3.0e-12
Identity = 37/71 (52.11%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 17  EQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDFE 76
           EQL  +F+ HD +KDG LS  ELT+AF  +G+F PS+RA  AL H+D +RDGF+ E +F 
Sbjct: 30  EQLKDVFKKHDTNKDGLLSREELTKAFSSLGSFFPSWRASRALSHVDKNRDGFVDENEFS 89

Query: 77  LLVNYAEQRKY 88
            LV Y  Q  Y
Sbjct: 90  DLVRYVAQLGY 100

[Close]

BLAST of HG10014116 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 48.9 bits (115), Expect = 2.5e-06
Identity = 21/61 (34.43%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 15  TREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKD 74
           ++E+L  +F + D+D DG++S  EL   F  +G ++    A+ A+  +D+D DG +G +D
Sbjct: 61  SKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFED 120

Query: 75  F 76
           F
Sbjct: 121 F 121

[Close]

BLAST of HG10014116 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 40.8 bits (94), Expect = 6.7e-04
Identity = 17/54 (31.48%), Postives = 31/54 (57.41%), Query Frame = 0


Query: 22 IFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDF 76
          +F+  D D DG+++T EL E+F+ +G  +P       +  ID + DG +  ++F
Sbjct: 9  VFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF 62

[Close]

BLAST of HG10014116 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 40.4 bits (93), Expect = 8.8e-04
Identity = 20/61 (32.79%), Postives = 31/61 (50.82%), Query Frame = 0


Query: 15 TREQLVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKD 74
          T  +L  +F   D + DG++S +EL   F+ +G           L  ID D DGFI +++
Sbjct: 17 TDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEE 76

Query: 75 F 76
          F
Sbjct: 77 F 77

[Close]

BLAST of HG10014116 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 40.4 bits (93), Expect = 8.8e-04
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0


Query: 19 LVVIFRNHDNDKDGRLSTAELTEAFRYIGAFLPSYRARHALFHIDSDRDGFIGEKDFELL 78
          L  +F   D ++DGR+S  EL ++F+ +G  +    A  A+   D D DG +   +F LL
Sbjct: 23 LEAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALL 82

Query: 79 V 80
          +
Sbjct: 83 I 83

The following BLAST results are available for this feature:

BLAST of HG10014116 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0064376.1	1.8e-35	82.22	putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 pu...	[more]
KGN55185.1	1.5e-34	82.22	hypothetical protein Csa_012915 [Cucumis sativus]	[more]
KAG7024248.1	6.5e-33	82.22	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6591370.1	4.2e-32	82.95	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6608481.1	1.5e-29	76.67	Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037808.1...	[more]

BLAST of HG10014116 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39419	1.2e-05	38.81	Polcalcin Bet v 4 OS=Betula pendula OX=3505 GN=BETV4 PE=1 SV=1	[more]
Q8L3R2	3.5e-05	34.43	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q0DJV6	1.0e-04	37.93	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8VWY6	2.9e-04	38.18	Polcalcin Nic t 1 OS=Nicotiana tabacum OX=4097 GN=Nict1 PE=1 SV=1	[more]

BLAST of HG10014116 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VDB9	8.8e-36	82.22	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0KZR6	7.5e-35	82.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1	[more]
A5AWP8	1.4e-12	47.62	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1	[more]
A0A5N6RQV2	1.4e-12	53.33	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1	[more]
W9RZZ8	3.0e-12	52.11	Putative calcium-binding protein CML27 OS=Morus notabilis OX=981085 GN=L484_0063...	[more]

BLAST of HG10014116 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	2.5e-06	34.43	calmodulin-like 41	[more]
AT4G03290.1	6.7e-04	31.48	EF hand calcium-binding protein family	[more]
AT1G73630.1	8.8e-04	32.79	EF hand calcium-binding protein family	[more]
AT1G76640.1	8.8e-04	32.79	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 21..44 e-value: 4.4E-5 score: 23.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 12.170192
None	No IPR available	GENE3D	1.10.238.10		coord: 1..86 e-value: 3.3E-10 score: 42.1
None	No IPR available	PANTHER	PTHR23050:SF451	EF-HAND PAIR PROTEIN	coord: 12..85
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 12..85
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 63..75
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 14..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014116.1	HG10014116.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding