HG10014112 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10014112
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationChr02: 7654302 .. 7657594 (+)
RNA-Seq ExpressionHG10014112
SyntenyHG10014112
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGTTCTTGTAACTGGGGCGTCTGGGTATCTGGGCGGAAGGCTGTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTCGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTGCTCTTCCTCATGATCCTTCTGCTCTTGAGCTCGTTCATGGTGATATCACCGACTACCAGTCGCTTCTAGAGGCATGCTCCGGCTGCCATGTCGTCTTCCACGCGGCTGCAATGGTCGAGCCATGGCTTCCTGATCCATCGAAATTCATCTCTGTAAGTACGCAGTTTTACATCTCAGATTTCTTCAAAATGTTGATTGAAACCGAGAAAAAGAAAATTTCCCGGAAAATGTGTCGAGTTCTAGGTTTTGGTTCAGTTCGTCGGATTTCTTTCAAATTTTTAAGAAATAACGAATTATCCGGTTGTTCATGTTAGGTCAATGTACGAGGGTTGGAGAACGTGTTGCAAGCGGTTAGAGAGACGAAGACAATCGAGAAGATTATCTATACGTCGTCCTTTTTCGCGCTTGGTTCAACCGATGGATATGTTGCCGATGAAAAACAGGTACTATTAGGATTTAAATTTTCAGACAATTATTCTTCCTCCTTGCTTTTGTGATACATGGTGATCTTCATTGATGTTTCCAAATTTCTCTGAAAGCGACTGTAATGACAGGTTCACCACGAAAAGTTCTTTTGTACGGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCTGCATCTGAGGGCATTCCGATAGTGCCGGTTTATCCCGGAGTTATCTATGGTGCTGGAAAAGTCACGGCTGGGAATGTTATTGCCCGCATGGTAATGTCTGCTCTTTCTCTGATAAATATCTGTTGGATTCGTGTGGTGTCTCAAGTTAGTGAGTCCTAGATTAACCAGCAAAAGATCAAAGTTCATTCAGTGGAATGACACTCATTTTGAATTCAGACTCCATTTTGGTGACAAAATAATCATGGACAAGAAAGTTGTTTCTGGAAATCCCAAGTTAAAATCACTTATGAGTGAGCTATAACTCCTCAAATGAGTTTACTAATGACTGGCCACTTCCTACTTTGGTTCTGGTTATTCTTTAAGTGTGAGTTTGACCTGACTTTTATAGCAGTGAAAATTGAAATACTTTTTTAGCTCACACTTTGACTTGAGCTAGTTTTGTTACATAGTCAAAGTGATTTTAGAAAATTACCAAGTTGCTTAAAAGCACCTCTAAATACTTGGGTGTTTGGCTCACCAACCTTATAAGTTAGTGTTAAATAACTTATTTTTAATAGTGTTCACTATTGAAGTCTGCACATTTATCGATGAACTTCATCTTTCTTTCTTTCTCTCTTTTGCACATTTATTGAGGAACTCTATTTTACAACTTTGCACCCCAAACACAAATTTCCTATCTCCGACATATTAACTCCAGACTTCAGAACTCAACTCAGTGTCCTAAGCACTCCTAGGGCCGTTTGACCCACCTAACAACTCAACTTCAATAGTAAACACTATTGAAGCTTGCACATTTATCGAATAACTCTATCTTTCCATTTTTTTTTCTATTTTTCACATTTATCAATGAACTTCATCTTACTTTCTTAATCTACATTTCACAATTATCGAGAAATTCCATCTTATAACTTTACACTCCAAACACAAACTTATTAACTCCACTCATTGGGCCAAAAGTCCCTTCAGTATTTTTGTTTCACTTGTAGATAAAATGTTTCATTTAACAATACTACTCTAGAAAGAAAGACATTTTTACTTTTTCTAAAAGGTTTTAAGTTTGAATCCCACGCAAGAAATATAATATTCTCAACACTGTTTTAAAAGGTTATCTCAAACACACTCTTCATTTCTTAATGATGTTCATAAATGCTCTTTATAACCTTTTTGAGGATTTTGTGAAGAAATCTGGTGAATAAATACAATTAATGAGGCGGTTAAATGCATTAGACAGAATATTATCATTAGCCTTTCTCCTCTTCATTGAATTGATTATTCTTAGGCTATTTGGCTAGGCCAATGCAAATTTGGTTAACTAAAATAAATCCTTTCTCTTTGGCTTATTTATTTATTCTTTTAGTGACCGGGTGCCAGGACAACTTGCGTGCACCTTGATTAATCTCAATGGATAACTCCCGACTCTACAACGTTTGAGTTCCAAGAAAACTCGTAAGATATTAAATCCTAGGTGGGTAGTCACCTTGGATTGAACCCATTCCCTCTAAGTCATTTTATTATACCCATGATCCTTATTGATCACTAAACCTAACCGTGTTACCTCTTTGGCTCTTTCCCTTTCTCTTTGAGCAGACTATTCTTCCCCTTCCTATCCTTTCTTGTCTGTTATCCTACTAATCATTTGAGAACTGCGGAAGTTATGCAAATTAGATTTTGGTAAAAAAAAAAAAAATTATTCTTCAATAAAAAGAATATGCTTATCCATCTCTTGAGAGCCCTTGTGAAACTGAAATGGGGAAATTTTTGGAATTTCCTTACTTATTTGTATGAAATCGATACATTTTAATCTGTCAAGTACTTTACTGATCATTATCTGTTATAAGTATTTGACTTGGCTTTCTTTCACAATGGGCCATTGTTGTTATTTCTAATCAGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGATAAGTATTCTTTTAGCCATGTCGATGATGTGGTAGAAGGGCATATTGCAGCAATGCATAAAGGTCGTGTAGGCGAAAGATATCTTCTAACAGGAGAAAATGCATCATTTATGGACGTTTTTGACACAGCTGCAGCCATTACCGGTACAAGAAAACCAATCTTTAACATTCCATTGTGGTTAATTGAAGCCTATGGCTGGGTTTCAGTTTTGATCTCTCGCATCACAGGGAAACTTCCTCTCATCAGCCCCCCGGTATGCACTCTCATGGTTCTATATTTATGTTAAATTGCTTGCATATCTCTTGTACTTAACATGGACAGCAATCAAATAGTTTAGGATTTTGCACCGACCATAGCACCCTATTCAATGGTTGAAACGCTTAGGCTGTTACTATGTTAATAAGTAACATAATTTGGAGTTGAAGTGACACTCTTTTTTGTTTTGTTTTTCTATTGTTGGTGCAGACTGTGAAGGTTTTGAGACATCAGTGGGCATATTCTTGTGACAAGGCAAAACAAGAACTGGATTATAACCCCAGAAGTTTAAAAGAAGGATTAGAAGAGATGCTACCATGGCTAAAGAGCTTGGGTTTGATAAAATATTAA

mRNA sequence

ATGAAGGTTCTTGTAACTGGGGCGTCTGGGTATCTGGGCGGAAGGCTGTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTCGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTGCTCTTCCTCATGATCCTTCTGCTCTTGAGCTCGTTCATGGTGATATCACCGACTACCAGTCGCTTCTAGAGGCATGCTCCGGCTGCCATGTCGTCTTCCACGCGGCTGCAATGGTCGAGCCATGGCTTCCTGATCCATCGAAATTCATCTCTGTCAATGTACGAGGGTTGGAGAACGTGTTGCAAGCGGTTAGAGAGACGAAGACAATCGAGAAGATTATCTATACGTCGTCCTTTTTCGCGCTTGGTTCAACCGATGGATATGTTGCCGATGAAAAACAGGTTCACCACGAAAAGTTCTTTTGTACGGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCTGCATCTGAGGGCATTCCGATAGTGCCGGTTTATCCCGGAGTTATCTATGGTGCTGGAAAAGTCACGGCTGGGAATGTTATTGCCCGCATGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGATAAGTATTCTTTTAGCCATGTCGATGATGTGGTAGAAGGGCATATTGCAGCAATGCATAAAGGTCGTGTAGGCGAAAGATATCTTCTAACAGGAGAAAATGCATCATTTATGGACGTTTTTGACACAGCTGCAGCCATTACCGGTACAAGAAAACCAATCTTTAACATTCCATTGTGGTTAATTGAAGCCTATGGCTGGGTTTCAGTTTTGATCTCTCGCATCACAGGGAAACTTCCTCTCATCAGCCCCCCGACTGTGAAGGTTTTGAGACATCAGTGGGCATATTCTTGTGACAAGGCAAAACAAGAACTGGATTATAACCCCAGAAGTTTAAAAGAAGGATTAGAAGAGATGCTACCATGGCTAAAGAGCTTGGGTTTGATAAAATATTAA

Coding sequence (CDS)

ATGAAGGTTCTTGTAACTGGGGCGTCTGGGTATCTGGGCGGAAGGCTGTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTCGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTGCTCTTCCTCATGATCCTTCTGCTCTTGAGCTCGTTCATGGTGATATCACCGACTACCAGTCGCTTCTAGAGGCATGCTCCGGCTGCCATGTCGTCTTCCACGCGGCTGCAATGGTCGAGCCATGGCTTCCTGATCCATCGAAATTCATCTCTGTCAATGTACGAGGGTTGGAGAACGTGTTGCAAGCGGTTAGAGAGACGAAGACAATCGAGAAGATTATCTATACGTCGTCCTTTTTCGCGCTTGGTTCAACCGATGGATATGTTGCCGATGAAAAACAGGTTCACCACGAAAAGTTCTTTTGTACGGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCTGCATCTGAGGGCATTCCGATAGTGCCGGTTTATCCCGGAGTTATCTATGGTGCTGGAAAAGTCACGGCTGGGAATGTTATTGCCCGCATGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGATAAGTATTCTTTTAGCCATGTCGATGATGTGGTAGAAGGGCATATTGCAGCAATGCATAAAGGTCGTGTAGGCGAAAGATATCTTCTAACAGGAGAAAATGCATCATTTATGGACGTTTTTGACACAGCTGCAGCCATTACCGGTACAAGAAAACCAATCTTTAACATTCCATTGTGGTTAATTGAAGCCTATGGCTGGGTTTCAGTTTTGATCTCTCGCATCACAGGGAAACTTCCTCTCATCAGCCCCCCGACTGTGAAGGTTTTGAGACATCAGTGGGCATATTCTTGTGACAAGGCAAAACAAGAACTGGATTATAACCCCAGAAGTTTAAAAGAAGGATTAGAAGAGATGCTACCATGGCTAAAGAGCTTGGGTTTGATAAAATATTAA

Protein sequence

MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSLLEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFALGSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTAGNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENASFMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAYSCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY
Homology
BLAST of HG10014112 vs. NCBI nr
Match: XP_038898413.1 (putative dihydroflavonol 4-reductase [Benincasa hispida])

HSP 1 Score: 663.3 bits (1710), Expect = 1.1e-186
Identity = 324/335 (96.72%), Postives = 331/335 (98.81%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MKVL+TGASGYLGGRLCRALL+RGFSVRALVRPTSDLS+LPHDPSALELVHGDITDYQSL
Sbjct: 1   MKVLLTGASGYLGGRLCRALLDRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVR+TKTIEKIIYTSSFFAL
Sbjct: 61  LEACWGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRKTKTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA
Sbjct: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F++VFDTAAAITGTRKP+FNIPLW IEAYGWVSV ISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 241 FVEVFDTAAAITGTRKPMFNIPLWFIEAYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY
Sbjct: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 335

BLAST of HG10014112 vs. NCBI nr
Match: XP_011654104.1 (uncharacterized protein LOC101223178 isoform X1 [Cucumis sativus] >KAE8649859.1 hypothetical protein Csa_012339 [Cucumis sativus])

HSP 1 Score: 647.9 bits (1670), Expect = 4.8e-182
Identity = 315/335 (94.03%), Postives = 324/335 (96.72%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLS+LPHDPSALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGL+NVLQAVRETKTIEKIIYTSSFFAL
Sbjct: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVA E QVHHEKFFCTEYEKSKA ADKIALQAASEGIPIVPVYPGVIYG GKVTA
Sbjct: 121 GSTDGYVAVESQVHHEKFFCTEYEKSKATADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNV+ARMLIERFNGRLPGYLGQG DK+SFSHVDDVVEGHI AM KGRVGERYLLTGENAS
Sbjct: 181 GNVVARMLIERFNGRLPGYLGQGKDKFSFSHVDDVVEGHIVAMQKGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F++VFD AAAITGT+KPIFNIPLWLIE YGWVSV ISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 241 FVEVFDAAAAITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAKQELDYNPRSLKEGLEEML WLKSLGLIKY
Sbjct: 301 SCEKAKQELDYNPRSLKEGLEEMLAWLKSLGLIKY 335

BLAST of HG10014112 vs. NCBI nr
Match: XP_008452530.1 (PREDICTED: putative dihydroflavonol-4-reductase [Cucumis melo] >KAA0064373.1 putative dihydroflavonol-4-reductase [Cucumis melo var. makuwa] >TYK20214.1 putative dihydroflavonol-4-reductase [Cucumis melo var. makuwa])

HSP 1 Score: 640.6 bits (1651), Expect = 7.7e-180
Identity = 311/335 (92.84%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           M +LVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLS+LPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGL+NVLQAVRETKTIEKIIYTSSFFAL
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVA E QVHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYG GK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNV+ARMLIERFNGRLPGYLGQGNDK+SFSH+DDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F++VFDTAAAITGT+KPIFNIPLWLIE YGWV V ISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAKQELDYNPRSLKEGLEEML WLKS+GLIKY
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEMLAWLKSVGLIKY 352

BLAST of HG10014112 vs. NCBI nr
Match: XP_023525575.1 (uncharacterized protein LOC111789148 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 637.1 bits (1642), Expect = 8.5e-179
Identity = 306/335 (91.34%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MKVLVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLS+LPHDPSALELV+GD+TDYQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSFFAL
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADE QVHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG+GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIARMLIERFN RLPGY+GQG+D+YSFSHVDDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNWRLPGYIGQGSDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F+DVFDTAAAITGTR+P+FNIPLWLIEAYGWVSV ISRITGKLPLISPP V VLRHQWAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVFISRITGKLPLISPPVVSVLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAK+ELDYNPRSLKEGLEEM+PWLKSLGLIKY
Sbjct: 301 SCEKAKEELDYNPRSLKEGLEEMVPWLKSLGLIKY 335

BLAST of HG10014112 vs. NCBI nr
Match: XP_022159877.1 (uncharacterized protein LOC111026159 [Momordica charantia])

HSP 1 Score: 636.3 bits (1640), Expect = 1.4e-178
Identity = 304/335 (90.75%), Postives = 325/335 (97.01%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLCRALLNRG+SVRAL+RPTSDLS+LPHDP+ALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGYSVRALIRPTSDLSSLPHDPAALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEACSGCHVVFHAAA+VEPWLPDPSKFISVNVRGL+NVLQAV ET+TIEKIIYTSSFFAL
Sbjct: 61  LEACSGCHVVFHAAALVEPWLPDPSKFISVNVRGLKNVLQAVTETRTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADE QVHHEKFFCTEYEKSKAIADKIAL AASEGIPIVPVYPGVIYG+GKVTA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALHAASEGIPIVPVYPGVIYGSGKVTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIARM+IERFN RLPGYLG+GNDK+SFSHVDDVVEGH+AA+ +GRVGERYLLTGENAS
Sbjct: 181 GNVIARMMIERFNWRLPGYLGRGNDKFSFSHVDDVVEGHLAALQRGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F+DVFD AAAITGTR+PIF IPLWLIEAYGWVS+ ISRITGKLPLISPPTV  LRHQWAY
Sbjct: 241 FVDVFDIAAAITGTRRPIFKIPLWLIEAYGWVSIFISRITGKLPLISPPTVNPLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAK+ELDYNPRSL+EGLEEMLPWLKSLGLIKY
Sbjct: 301 SCEKAKEELDYNPRSLREGLEEMLPWLKSLGLIKY 335

BLAST of HG10014112 vs. ExPASy Swiss-Prot
Match: P73212 (Putative dihydroflavonol 4-reductase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=dfrA PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-38
Identity = 112/338 (33.14%), Postives = 162/338 (47.93%), Query Frame = 0

Query: 5   VTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSLLEAC 64
           VTG +G++G  L R LL +G+ VRALVR +S    L + P  ++ V GD+ D   L +  
Sbjct: 15  VTGGTGFVGANLVRHLLEQGYQVRALVRASSRPDNLQNLP--IDWVVGDLND-GDLHQQM 74

Query: 65  SGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFALG-ST 124
            GC  +FH AA    W  D       NV G  N+L A  +   IE+ +YTSS  A+G   
Sbjct: 75  QGCQGLFHVAAHYSLWQKDREALYRSNVLGTRNIL-ACAQKAGIERTVYTSSVAAIGVKG 134

Query: 125 DGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKV---TA 184
           DG  ADE      +     Y++SK  A++ AL AA +G  IV V P    G   +     
Sbjct: 135 DGQRADESYQSPVEKLIGAYKQSKYWAEQEALTAAQQGQDIVIVNPSTPIGPWDIKPTPT 194

Query: 185 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRV----GERYLLTG 244
           G +I R L      ++P Y+  G    +   V DV  GH+ A  +G+     G+RY+L  
Sbjct: 195 GEIILRFL----RRQMPAYVNTG---LNLIDVRDVAAGHLLAWQRGKTALTRGDRYILGH 254

Query: 245 ENASFMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRH 304
           EN S   +    + ITG   P   +PLWL   + WV   +    G+ P +    VK+   
Sbjct: 255 ENISLQGILAHLSTITGLPAPKNTVPLWLPLTFAWVEEKVLAPLGRSPSVPMDGVKMSAQ 314

Query: 305 QWAYSCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIK 335
           +  Y   KA QEL     S+K+ L + + W ++ G +K
Sbjct: 315 EMYYDASKAVQELGLPQSSIKQALADAVHWFQNHGYVK 341

BLAST of HG10014112 vs. ExPASy Swiss-Prot
Match: H1ZZB0 (Aurachin B dehydrogenase OS=Stigmatella aurantiaca OX=41 GN=auaH PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.5e-24
Identity = 97/329 (29.48%), Postives = 151/329 (45.90%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           M+  VTG SGYLG  L  AL+ RG SVRALVR  S+ +A        + + G +   ++L
Sbjct: 1   MRTFVTGGSGYLGRNLLSALVARGISVRALVR--SEEAAQKVQALGAQPILGTLEHRETL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
            E  +GC V+FHAAA+      D ++F   NV G E VL A R+ + I+++++ S+   L
Sbjct: 61  KEGMAGCDVLFHAAALTSARATD-AEFHRANVLGTETVLAAARDAR-IQRMVHVSTEAVL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
                 +  ++     K     Y  +KA A+++ LQA   G   V V P  I+GA     
Sbjct: 121 ADGRPLLQVDESHPLPKRPFAGYPATKAQAEQLVLQANGPGFTTVVVRPRFIWGAD---- 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLT-GENA 240
                  LI+    +   ++  G    S  HV +V EG + A  +G  GE Y LT G   
Sbjct: 181 DTAFLPQLIDAIRTKRFRWVDGGRYLTSTCHVANVCEGMLLAAERGPGGEVYFLTDGAPV 240

Query: 241 SFMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVK----VLR 300
                        G +  + NIP     A   +   + R    +P    P ++    +L 
Sbjct: 241 ELRSFLTLLLETQGIKAEVGNIPFQAARAAAHLGESLWR--ALVPQARAPALRLAVYLLG 300

Query: 301 HQWAYSCDKAKQELDYNPR-SLKEGLEEM 324
            +   + DKA++EL Y  R + ++GL+ +
Sbjct: 301 REVTLNDDKARRELGYAGRVTHQQGLDAL 319

BLAST of HG10014112 vs. ExPASy Swiss-Prot
Match: P51103 (Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-21
Identity = 95/350 (27.14%), Postives = 155/350 (44.29%), Query Frame = 0

Query: 3   VLVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSALPHDPSALELVHGDITD 62
           V VTGA+G++G  L   LL RG+ VRA VR   D      L  LP   + L L   D+T 
Sbjct: 9   VCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLTLWKADLTQ 68

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLENVLQAVRETKTIEKIIYTS 122
             S  EA  GCH VFH A  ++    DP ++ I   + G+ +++++  + KT++K++YTS
Sbjct: 69  EGSFDEAIEGCHGVFHVATPMDFESKDPENEIIKPTIEGILSIIRSCAKAKTVKKLVYTS 128

Query: 123 SFFALG--STDGYVADEKQ------VHHEKFFCTEYEKSKAIADKIALQAASE-GIPIVP 182
           S   +    T   V DE        ++ +K     Y  SK +A+K A++AA E  I  V 
Sbjct: 129 SAGTVNVQETQLPVYDESHWSDLDFIYSKKMTAWMYFVSKTLAEKAAMEAAKENNIDFVS 188

Query: 183 VYPGVIYGAGKVTAGNVIARMLIERFNGRLPGY--LGQGNDKYSFSHVDDVVEGHIAAMH 242
           + P ++ G              +   NG    Y  + QG     + H+DD+ E HI    
Sbjct: 189 IIPPLVVGPFINPTFPPSLITALSLINGAESHYSIIKQG----QYVHLDDLCECHIFLYE 248

Query: 243 KGRVGERYLLTGENASFMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLP 302
                 RY+ + ++A+   +    A +   + P +++P              + I  +LP
Sbjct: 249 NPEAKGRYICSKQDATIHQL----ARMIKQKWPEYHVP-----------TQFAGIDEELP 308

Query: 303 LISPPTVKVLRHQWAYSCD------------KAKQELDYNPRSLKEGLEE 323
            +S  + K++   + +  D            K K  L Y+   +K+GL E
Sbjct: 309 TVSFSSKKLIDMGFKFKYDLEDMFKGAIDSCKEKGFLPYSTNEVKKGLFE 339

BLAST of HG10014112 vs. ExPASy Swiss-Prot
Match: P9WQP6 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1137 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.4e-21
Identity = 98/349 (28.08%), Postives = 164/349 (46.99%), Query Frame = 0

Query: 2   KVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSLL 61
           +VLVTG +G++G  L   LL+RG  VR+  R  S L A P     LE++ GDITD     
Sbjct: 16  RVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQ----LEVLQGDITDADVCA 75

Query: 62  EACSGCHVVFHAAAMVE----PWLPDP--SKFISVNVRGLENVLQAVRETKTIEKIIYTS 121
            A  G   +FH AA++E      + D    +  +VNV G EN+L A  +   +++ +YTS
Sbjct: 76  AAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHA-GQRAGVQRFVYTS 135

Query: 122 SFFALGSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIAL-QAASEGIPIVPVYPGVIYG 181
           S   +         ++ + +   F   Y ++K +A++  L Q   +G+    + P  I+G
Sbjct: 136 SNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGVDGMLTCAIRPSGIWG 195

Query: 182 AGKVTAGNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHI-AAMH----KGRVG 241
            G  T   +  ++      G +   +G+ + +   S+V +++ G I AA H        G
Sbjct: 196 NGDQT---MFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVPDGTAPG 255

Query: 242 ERYLLTGENASFMDVFDTAAAI---TGTRKPIFNIP----LWLIEAYGWVSVLISRITGK 301
           + Y +   +A  +++F+ A  +    G R P   I      W++   GW   L  R    
Sbjct: 256 QAYFI--NDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMT--GW-QRLHFRFGFP 315

Query: 302 LPLISPPTVKVLRHQWAYSCDKAKQELDYNPR-SLKEGLEEMLPWLKSL 331
            PL+ P  V+ L     +S  KA+++L Y P  + ++ L E LP+  SL
Sbjct: 316 APLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQALTECLPYYVSL 351

BLAST of HG10014112 vs. ExPASy Swiss-Prot
Match: P9WQP7 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1106c PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.4e-21
Identity = 98/349 (28.08%), Postives = 164/349 (46.99%), Query Frame = 0

Query: 2   KVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSLL 61
           +VLVTG +G++G  L   LL+RG  VR+  R  S L A P     LE++ GDITD     
Sbjct: 16  RVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQ----LEVLQGDITDADVCA 75

Query: 62  EACSGCHVVFHAAAMVE----PWLPDP--SKFISVNVRGLENVLQAVRETKTIEKIIYTS 121
            A  G   +FH AA++E      + D    +  +VNV G EN+L A  +   +++ +YTS
Sbjct: 76  AAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHA-GQRAGVQRFVYTS 135

Query: 122 SFFALGSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIAL-QAASEGIPIVPVYPGVIYG 181
           S   +         ++ + +   F   Y ++K +A++  L Q   +G+    + P  I+G
Sbjct: 136 SNSVVMGGQNIAGGDETLPYTDRFNDLYTETKVVAERFVLAQNGVDGMLTCAIRPSGIWG 195

Query: 182 AGKVTAGNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHI-AAMH----KGRVG 241
            G  T   +  ++      G +   +G+ + +   S+V +++ G I AA H        G
Sbjct: 196 NGDQT---MFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVPDGTAPG 255

Query: 242 ERYLLTGENASFMDVFDTAAAI---TGTRKPIFNIP----LWLIEAYGWVSVLISRITGK 301
           + Y +   +A  +++F+ A  +    G R P   I      W++   GW   L  R    
Sbjct: 256 QAYFI--NDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMT--GW-QRLHFRFGFP 315

Query: 302 LPLISPPTVKVLRHQWAYSCDKAKQELDYNPR-SLKEGLEEMLPWLKSL 331
            PL+ P  V+ L     +S  KA+++L Y P  + ++ L E LP+  SL
Sbjct: 316 APLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQALTECLPYYVSL 351

BLAST of HG10014112 vs. ExPASy TrEMBL
Match: A0A5A7V8B0 (Putative dihydroflavonol-4-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003510 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 311/335 (92.84%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           M +LVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLS+LPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGL+NVLQAVRETKTIEKIIYTSSFFAL
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVA E QVHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYG GK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNV+ARMLIERFNGRLPGYLGQGNDK+SFSH+DDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F++VFDTAAAITGT+KPIFNIPLWLIE YGWV V ISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAKQELDYNPRSLKEGLEEML WLKS+GLIKY
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEMLAWLKSVGLIKY 352

BLAST of HG10014112 vs. ExPASy TrEMBL
Match: A0A1S3BV78 (putative dihydroflavonol-4-reductase OS=Cucumis melo OX=3656 GN=LOC103493529 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 311/335 (92.84%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           M +LVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLS+LPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGL+NVLQAVRETKTIEKIIYTSSFFAL
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVA E QVHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYG GK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNV+ARMLIERFNGRLPGYLGQGNDK+SFSH+DDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F++VFDTAAAITGT+KPIFNIPLWLIE YGWV V ISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAKQELDYNPRSLKEGLEEML WLKS+GLIKY
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEMLAWLKSVGLIKY 352

BLAST of HG10014112 vs. ExPASy TrEMBL
Match: A0A6J1E109 (uncharacterized protein LOC111026159 OS=Momordica charantia OX=3673 GN=LOC111026159 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.0e-179
Identity = 304/335 (90.75%), Postives = 325/335 (97.01%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLCRALLNRG+SVRAL+RPTSDLS+LPHDP+ALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGYSVRALIRPTSDLSSLPHDPAALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           LEACSGCHVVFHAAA+VEPWLPDPSKFISVNVRGL+NVLQAV ET+TIEKIIYTSSFFAL
Sbjct: 61  LEACSGCHVVFHAAALVEPWLPDPSKFISVNVRGLKNVLQAVTETRTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADE QVHHEKFFCTEYEKSKAIADKIAL AASEGIPIVPVYPGVIYG+GKVTA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALHAASEGIPIVPVYPGVIYGSGKVTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIARM+IERFN RLPGYLG+GNDK+SFSHVDDVVEGH+AA+ +GRVGERYLLTGENAS
Sbjct: 181 GNVIARMMIERFNWRLPGYLGRGNDKFSFSHVDDVVEGHLAALQRGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F+DVFD AAAITGTR+PIF IPLWLIEAYGWVS+ ISRITGKLPLISPPTV  LRHQWAY
Sbjct: 241 FVDVFDIAAAITGTRRPIFKIPLWLIEAYGWVSIFISRITGKLPLISPPTVNPLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAK+ELDYNPRSL+EGLEEMLPWLKSLGLIKY
Sbjct: 301 SCEKAKEELDYNPRSLREGLEEMLPWLKSLGLIKY 335

BLAST of HG10014112 vs. ExPASy TrEMBL
Match: A0A6J1J4E6 (uncharacterized protein LOC111481147 OS=Cucurbita maxima OX=3661 GN=LOC111481147 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 2.7e-178
Identity = 305/335 (91.04%), Postives = 325/335 (97.01%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MKVLVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLS+LPHDPSALELV+GD+T+YQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTNYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSFFAL
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADE QVHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG+GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIARMLIERFN RLPGY+GQG D+YSFSHVDDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNWRLPGYIGQGTDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F+DVFDTAAAITGTR+P+FNIPLWLIEAYGWVSV ISRITGKLPLISPP V VLRHQWAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVYISRITGKLPLISPPVVSVLRHQWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAK+ELDYNPRSLKEGLEEM+PWLKSLGLIKY
Sbjct: 301 SCEKAKEELDYNPRSLKEGLEEMVPWLKSLGLIKY 335

BLAST of HG10014112 vs. ExPASy TrEMBL
Match: A0A6J1FLK2 (uncharacterized protein LOC111446335 OS=Cucurbita moschata OX=3662 GN=LOC111446335 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.7e-178
Identity = 303/335 (90.45%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MKVLVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLS+LPHDPSALELV+GD+TDYQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSFFAL
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDGYVADE QVHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG+GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
           GNVIAR+LIERFN RLPGY+GQG+D+YSFSHVDDVVEGHIAAM KGRVGERYLLTGENAS
Sbjct: 181 GNVIARLLIERFNWRLPGYIGQGSDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F+DVFDTAAAITGTR+P+FNIPLWLIEAYGWVSV+ISRITGKLPLISPP V VLRH+WAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVVISRITGKLPLISPPVVSVLRHEWAY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SC+KAK+ELDY PRSLKEGLEEM+PWLKSLGLIKY
Sbjct: 301 SCEKAKEELDYKPRSLKEGLEEMVPWLKSLGLIKY 335

BLAST of HG10014112 vs. TAIR 10
Match: AT4G33360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 546.2 bits (1406), Expect = 1.8e-155
Identity = 259/335 (77.31%), Postives = 300/335 (89.55%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 13  MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 72

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSFFAL
Sbjct: 73  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 132

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDG VA+E QVH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 133 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 192

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
            N++ARMLIERFNGRLPGY+G G D+YSFSHVDDVVEGH+AAM KGR+GERYLLTGENAS
Sbjct: 193 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 252

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F  VFD AA ITGT+KP F+IPLW I AYGW+SVLISR+TGKLPLISPPTV VLRHQW+Y
Sbjct: 253 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPPTVTVLRHQWSY 312

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLPWLKSLGLIKY 336
           SCDKAK EL YNPRSLKEGLEEMLPWLKSLG+I Y
Sbjct: 313 SCDKAKLELGYNPRSLKEGLEEMLPWLKSLGVIHY 344

BLAST of HG10014112 vs. TAIR 10
Match: AT4G33360.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 528.5 bits (1360), Expect = 4.0e-150
Identity = 251/325 (77.23%), Postives = 291/325 (89.54%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 1   MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSFFAL
Sbjct: 61  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 120

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDG VA+E QVH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 121 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 180

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
            N++ARMLIERFNGRLPGY+G G D+YSFSHVDDVVEGH+AAM KGR+GERYLLTGENAS
Sbjct: 181 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 240

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPPTVKVLRHQWAY 300
           F  VFD AA ITGT+KP F+IPLW I AYGW+SVLISR+TGKLPLISPPTV VLRHQW+Y
Sbjct: 241 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPPTVTVLRHQWSY 300

Query: 301 SCDKAKQELDYNPRSLKEGLEEMLP 326
           SCDKAK EL YNPRSLKEGLEEMLP
Sbjct: 301 SCDKAKLELGYNPRSLKEGLEEMLP 322

BLAST of HG10014112 vs. TAIR 10
Match: AT4G33360.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 461.8 bits (1187), Expect = 4.6e-130
Identity = 219/289 (75.78%), Postives = 258/289 (89.27%), Query Frame = 0

Query: 1   MKVLVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSALPHDPSALELVHGDITDYQSL 60
           MK+LVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 13  MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 72

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRETKTIEKIIYTSSFFAL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSFFAL
Sbjct: 73  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 132

Query: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180
           GSTDG VA+E QVH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 133 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 192

Query: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHKGRVGERYLLTGENAS 240
            N++ARMLIERFNGRLPGY+G G D+YSFSHVDDVVEGH+AAM KGR+GERYLLTGENAS
Sbjct: 193 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 252

Query: 241 FMDVFDTAAAITGTRKPIFNIPLWLIEAYGWVSVLISRITGKLPLISPP 290
           F  VFD AA ITGT+KP F+IPLW I AYGW+SVLISR+TGKLPLISPP
Sbjct: 253 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPP 298

BLAST of HG10014112 vs. TAIR 10
Match: AT1G09490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 3.4e-24
Identity = 85/261 (32.57%), Postives = 124/261 (47.51%), Query Frame = 0

Query: 3   VLVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSALPHDPSALELVHGDITD 62
           V VTGASGY+   + + LL RG++V A VR   D      L AL      L+L   D+ +
Sbjct: 8   VCVTGASGYIASWIVKLLLLRGYTVNATVRDPKDKKKTEHLLALDGAKERLKLFKADLLE 67

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLENVLQAVRETKTIEKIIYTS 122
             S  +A  GC  VFH A+ V   + DP ++ I   ++G  NVL   ++  +++++I TS
Sbjct: 68  ESSFDQAIDGCDAVFHTASPVLFTVTDPQTELIDPALKGTINVLNTCKQVSSVKRVILTS 127

Query: 123 SFFALGSTDGYVADEKQVHHEKFF-----CTE----YEKSKAIADKIALQAASE-GIPIV 182
           S  A+ S    +     V  E FF     C E    Y  SK +A+  A Q A + GI +V
Sbjct: 128 STAAVLSRQPPIGPNDLV-DETFFSDPSLCRETKNWYSLSKILAENAAWQFAKDNGIDMV 187

Query: 183 PVYPGVIYGAGKVTAGNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHK 242
            + PG I G       N+   ++++  NG+ P         Y FS V DV   HI A+  
Sbjct: 188 VLNPGFICGPLLQPTLNMSVELIVDFINGKNP----FNKRYYRFSDVRDVALVHIKALET 247

Query: 243 GRVGERYLLTGENASFMDVFD 247
                RY++ G N S  D+ D
Sbjct: 248 PSANGRYIIDGPNMSVNDIID 263

BLAST of HG10014112 vs. TAIR 10
Match: AT1G09480.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 1.3e-23
Identity = 84/261 (32.18%), Postives = 125/261 (47.89%), Query Frame = 0

Query: 3   VLVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSALPHDPSALELVHGDITD 62
           V VTGASGY+   + + LL RG++V+A VR  +D      L AL      L+L   D+ +
Sbjct: 55  VCVTGASGYIASWIVKLLLLRGYTVKATVRDLTDRKKTEHLLALDGAKERLKLFKADLLE 114

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLENVLQAVRETKTIEKIIYTS 122
             S  +A  GC  VFH A+ V   + DP ++ I   ++G  NVL   +ET ++ ++I TS
Sbjct: 115 ESSFEQAIEGCDAVFHTASPVFFTVKDPQTELIDPALKGTMNVLNTCKETPSVRRVILTS 174

Query: 123 SFFALGSTDGYVADEKQVHHEKFF-----CTE----YEKSKAIADKIALQAASE-GIPIV 182
           S  A+      V +   V  E FF     C E    Y  SK +A+  A + A + GI +V
Sbjct: 175 STAAVLFRQPPV-EASDVVDETFFSDPSLCRETKNWYPLSKILAENAAWEFAKDNGIDMV 234

Query: 183 PVYPGVIYGAGKVTAGNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMHK 242
            + PG I+G       N    ++++  NG+ P      +  Y F  V DV   HI A+  
Sbjct: 235 VLNPGFIFGPLLQPTLNFSVELIVDFINGKNP----FNSRFYRFVDVRDVALAHIKALET 294

Query: 243 GRVGERYLLTGENASFMDVFD 247
                RY++ G   S  D+ D
Sbjct: 295 PSANGRYIIDGPIMSVSDIID 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898413.11.1e-18696.72putative dihydroflavonol 4-reductase [Benincasa hispida][more]
XP_011654104.14.8e-18294.03uncharacterized protein LOC101223178 isoform X1 [Cucumis sativus] >KAE8649859.1 ... [more]
XP_008452530.17.7e-18092.84PREDICTED: putative dihydroflavonol-4-reductase [Cucumis melo] >KAA0064373.1 put... [more]
XP_023525575.18.5e-17991.34uncharacterized protein LOC111789148 [Cucurbita pepo subsp. pepo][more]
XP_022159877.11.4e-17890.75uncharacterized protein LOC111026159 [Momordica charantia][more]
Match NameE-valueIdentityDescription
P732122.3e-3833.14Putative dihydroflavonol 4-reductase OS=Synechocystis sp. (strain PCC 6803 / Kaz... [more]
H1ZZB09.5e-2429.48Aurachin B dehydrogenase OS=Stigmatella aurantiaca OX=41 GN=auaH PE=1 SV=1[more]
P511032.6e-2127.14Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1[more]
P9WQP63.4e-2128.083 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuber... [more]
P9WQP73.4e-2128.083 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase OS=Mycobacterium tuber... [more]
Match NameE-valueIdentityDescription
A0A5A7V8B03.7e-18092.84Putative dihydroflavonol-4-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BV783.7e-18092.84putative dihydroflavonol-4-reductase OS=Cucumis melo OX=3656 GN=LOC103493529 PE=... [more]
A0A6J1E1097.0e-17990.75uncharacterized protein LOC111026159 OS=Momordica charantia OX=3673 GN=LOC111026... [more]
A0A6J1J4E62.7e-17891.04uncharacterized protein LOC111481147 OS=Cucurbita maxima OX=3661 GN=LOC111481147... [more]
A0A6J1FLK27.7e-17890.45uncharacterized protein LOC111446335 OS=Cucurbita moschata OX=3662 GN=LOC1114463... [more]
Match NameE-valueIdentityDescription
AT4G33360.11.8e-15577.31NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G33360.34.0e-15077.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G33360.24.6e-13075.78NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09490.13.4e-2432.57NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09480.11.3e-2332.18NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 1..334
e-value: 1.3E-103
score: 348.5
NoneNo IPR availablePANTHERPTHR10366:SF624NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 3..335
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 3..335
NoneNo IPR availableCDDcd05228AR_FR_like_1_SDR_ecoord: 3..327
e-value: 1.48901E-136
score: 388.567
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 3..232
e-value: 6.9E-31
score: 107.5
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10014112.1HG10014112.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016487 farnesol metabolic process
biological_process GO:0009788 negative regulation of abscisic acid-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0047886 farnesol dehydrogenase activity
molecular_function GO:0003824 catalytic activity