HG10014088 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014088
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Ent-kaurene oxidase
Location	Chr02: 7457627 .. 7459894 (+)
RNA-Seq Expression	HG10014088
Synteny	HG10014088

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTGTTACTGATCCTATTGCTTCCATGCAGCTTCTCGTTAATACCCTTCAGGCTCCACCGTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTGATGCTTTCGGTGTTTCTCGTTGCTGATTGTAAAAAGAAACGACGGAATTTTCTCCCTCCAGTGCCGGGTAATGATCGCCTTCTTGTCTCTCTGTTTTCAATCATGGTGGAATTGATCGATTACTCTTTTGGATTATCATTCTGATTCTAGGGTTTTTAGGGAAAATGATGTGAAACTGATGATCTGATGAATTTGGGAATTTTGATTTGGTTTTTGTAGCGATTCCGGGGGTGCCATTGTTGGGGAATTTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCTCTTCCACTGTGATTGTTCTCAACACCGCTGAAGTTGCCAAAGAGGTATAATATCACATGTATTAGTTAGTAAATAAAATCACGGCTGTTTTCCGTCCAAAATTTCCCCTCTACTTCTGATTCTAACTGTACTCTGTTCTGGGCTTGGTTCTCTTAAGGCTATGGTGACGAGATACGCTTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTTACCATCTTGACGGCTGATAAATGCATGGTTGCTATGAGCGATTACAATGACTTTCACAAGATGGTAAAGAGATACATATTGGCGAATGTCTTGGGGGCTAATGCTCAGGTTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTATTTCCATTTGGGTTGAAACTAATAGTTTGATCTGTTTCATTTTCAGAAGAAACACAGACAAAGGAGAGACACCATGATTGAGAATATTACTAGGGAGTTGTTTGCTCATCTGAAGGATTTTCCATCAGAAACTGTTAATTTTCGAAAGATCTTCGAATCAGAACTCTTTCGGCTGGCGCTTAAAGAAGTGAGTCGATTCTGTTTTTCAATTAAATACAAAACTTGGATGTGTGTGGTAGATGGAAAGGAGGATGGATAAATAGCTAAGTTTTATGATTTCATTAACAGACACTTGGGAAAGATATTGAGTCGATATATGTCGATGGGCTCGGTACGACGTTGCCAAGGGAAGATCTGTTCAGAGTTTTAGTGATTGATCCAATGGAAGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAATAAGAATGTGGAGAATAGAATAAGGAAAATGGATTTTCGGAGGCGAATGACAATGAAGAGCTTAATTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGTAGTTCTTCCTTCCTTCTCTTGAATTGAAATGAGATTGTTTATCTGCTCTGTTTGACTTGGCTTGAAAAACAGGAAACGAATTGCTATGCTGATTTCTTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATAGCCATGTTACTTTGGGAGACCATCATTGAAACCTCAGACACTACACTGGTTGTAACAGAATGGGCTATGTATGAACTCGCAAAAGATCCGAAACGACAGGCAATTTCCAAACACACTACCATTTTTCTGTTTTCTGCTTGTCTTTCCAATTCTTAAGATCATCAAAACCAGTCAACATTCTTTAAAAACTTTTGACATTGCAGGACTATCTGTATCAGCATATACTGAGTGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTTTTTCATGAAACTCTTCGGAAGCATAGTCCAGTCCCAGTCGTCCCATTGCGATATGCGCATGAAGATACCCAATTAGGCGGGTTCTTTGTTCCTGCAGGGAGTGAGGTGAGCCATTCTAGAACCCTGAAACATCCCAGTGTAATTTGATATGTCAATGTCTGATGATCTTTCGTTTTCAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCCTGCACAACCATAGGAAAGATGGTTCAAGAGTTTGAGTGGGAGCTGAGGGAAGGGGAAGAGGACAAGGTGGACACTCTTGGCCTCACCGCTCGCAAGCTCCAGCCACTGCACTTAGTCGTAAAGCCGAGGATCAACTAA

mRNA sequence

ATGGCGGTTGTTACTGATCCTATTGCTTCCATGCAGCTTCTCGTTAATACCCTTCAGGCTCCACCGTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTGATGCTTTCGGTGTTTCTCGTTGCTGATTGTAAAAAGAAACGACGGAATTTTCTCCCTCCAGTGCCGGCGATTCCGGGGGTGCCATTGTTGGGGAATTTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCTCTTCCACTGTGATTGTTCTCAACACCGCTGAAGTTGCCAAAGAGGCTATGGTGACGAGATACGCTTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTTACCATCTTGACGGCTGATAAATGCATGGTTGCTATGAGCGATTACAATGACTTTCACAAGATGAAGAAACACAGACAAAGGAGAGACACCATGATTGAGAATATTACTAGGGAGTTGTTTGCTCATCTGAAGGATTTTCCATCAGAAACTGTTAATTTTCGAAAGATCTTCGAATCAGAACTCTTTCGGCTGGCGCTTAAAGAAACACTTGGGAAAGATATTGAGTCGATATATGTCGATGGGCTCGGTACGACGTTGCCAAGGGAAGATCTGTTCAGAGTTTTAGTGATTGATCCAATGGAAGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAATAAGAATGTGGAGAATAGAATAAGGAAAATGGATTTTCGGAGGCGAATGACAATGAAGAGCTTAATTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGAAACGAATTGCTATGCTGATTTCTTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATAGCCATGTTACTTTGGGAGACCATCATTGAAACCTCAGACACTACACTGGACTATCTGTATCAGCATATACTGAGTGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTTTTTCATGAAACTCTTCGGAAGCATAGTCCAGTCCCAGTCGTCCCATTGCGATATGCGCATGAAGATACCCAATTAGGCGGGTTCTTTGTTCCTGCAGGGAGTGAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCCTGCACAACCATAGGAAAGATGGTTCAAGAGTTTGAGTGGGAGCTGAGGGAAGGGGAAGAGGACAAGGTGGACACTCTTGGCCTCACCGCTCGCAAGCTCCAGCCACTGCACTTAGTCGTAAAGCCGAGGATCAACTAA

Coding sequence (CDS)

ATGGCGGTTGTTACTGATCCTATTGCTTCCATGCAGCTTCTCGTTAATACCCTTCAGGCTCCACCGTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTGATGCTTTCGGTGTTTCTCGTTGCTGATTGTAAAAAGAAACGACGGAATTTTCTCCCTCCAGTGCCGGCGATTCCGGGGGTGCCATTGTTGGGGAATTTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCTCTTCCACTGTGATTGTTCTCAACACCGCTGAAGTTGCCAAAGAGGCTATGGTGACGAGATACGCTTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTTACCATCTTGACGGCTGATAAATGCATGGTTGCTATGAGCGATTACAATGACTTTCACAAGATGAAGAAACACAGACAAAGGAGAGACACCATGATTGAGAATATTACTAGGGAGTTGTTTGCTCATCTGAAGGATTTTCCATCAGAAACTGTTAATTTTCGAAAGATCTTCGAATCAGAACTCTTTCGGCTGGCGCTTAAAGAAACACTTGGGAAAGATATTGAGTCGATATATGTCGATGGGCTCGGTACGACGTTGCCAAGGGAAGATCTGTTCAGAGTTTTAGTGATTGATCCAATGGAAGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAATAAGAATGTGGAGAATAGAATAAGGAAAATGGATTTTCGGAGGCGAATGACAATGAAGAGCTTAATTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGAAACGAATTGCTATGCTGATTTCTTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATAGCCATGTTACTTTGGGAGACCATCATTGAAACCTCAGACACTACACTGGACTATCTGTATCAGCATATACTGAGTGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTTTTTCATGAAACTCTTCGGAAGCATAGTCCAGTCCCAGTCGTCCCATTGCGATATGCGCATGAAGATACCCAATTAGGCGGGTTCTTTGTTCCTGCAGGGAGTGAGATCGCCGTGAACATTTACGCATGCAACATGGACAAGGATCATTGGGAAAGCCCTGAGGAATGGAAGCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTGCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAATGCTGATAGCCTGCACAACCATAGGAAAGATGGTTCAAGAGTTTGAGTGGGAGCTGAGGGAAGGGGAAGAGGACAAGGTGGACACTCTTGGCCTCACCGCTCGCAAGCTCCAGCCACTGCACTTAGTCGTAAAGCCGAGGATCAACTAA

Protein sequence

MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMKKHRQRRDTMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEDQIAMLLWETIIETSDTTLDYLYQHILSVCGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN

Homology

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BLAST of HG10014088 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 917.5 bits (2370), Expect = 4.7e-263
Identity = 462/519 (89.02%), Postives = 482/519 (92.87%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ SMQLL NTLQAPPYAAAAVLGGVSL+LSVFLVADC+KKRRNFLPPVPAIPG
Sbjct: 1   MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAIPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VP+LGNLLQLKEKKPHMTFARWAE YGPVYSIRTG+STVIVLNTAEVAKEAMVTRYASIS
Sbjct: 61  VPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYNDFHKM               KKHRQRRDTMIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FPSE+VNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI
Sbjct: 181 ELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK+VEN+IRKMDF+R+MTM+SLIEEQKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTMRSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 D+LYQHILS 
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDFLYQHILSA 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+KLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           M QEFEW+LREGEEDKVDTLGLTARKLQPLH+VVKPRIN
Sbjct: 481 MAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPRIN 519

[Close]

BLAST of HG10014088 vs. NCBI nr
Match: XP_008452488.1 (PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-kaurene oxidase [Cucumis melo var. makuwa] >TYK20237.1 ent-kaurene oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 896.3 bits (2315), Expect = 1.1e-256
Identity = 449/519 (86.51%), Postives = 475/519 (91.52%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ASMQLL NTL APPYAAAAVLGGVSL+LSVFLVADC+KKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTG+STVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKM               KKHRQRRD MIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FPS+TVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK+VEN+IR MDFRRRMTMKSLI+E K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDK+HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           MVQEFEW+LREGEE+KVDTLGLTARKLQPLH+V+KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of HG10014088 vs. NCBI nr
Match: NP_001267703.1 (ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kaurene oxidase [Cucumis sativus] >AFQ32799.1 ent-kaurene oxidase [Cucumis sativus] >KGN55152.1 hypothetical protein Csa_012319 [Cucumis sativus])

HSP 1 Score: 885.6 bits (2287), Expect = 2.0e-253
Identity = 444/519 (85.55%), Postives = 469/519 (90.37%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ASMQLL NT+ APPYAAAAVLGGVSL+LSVF VADC+KKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VP+LGNLLQLKEKKPH TFARWAETYG VYSIRTG+STVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKM               KKHRQRRD MIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FP +TVNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VEN+IR MDFRRRMTMK L+EE KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGSA LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKDHWESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           MVQEFEW+LREGEE+KVDTLGLTARKLQPLH+V+KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of HG10014088 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 424/503 (84.29%), Postives = 463/503 (92.05%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MA+  DP+ S+Q L++TLQAP YAAA++LG +S +L+ FLVAD +K RRNFLPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           +P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTG+STVIVLNTAEVAKEAMVTRYAS+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILT+DKCMVAMSDYNDFHKM               K+HRQRRDTMI+NI+ 
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           +LFAH+KD PSE+VNFR+IFESELFRLALKETLGKDIESIYVDGLG TLPREDLFRVLVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-TLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYL+WIPNK+VEN+IR+MDF RR+TM SLIEEQKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFCRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-DYLYQHILSVCGSAKLTEENLSQLPY 360
           YADFLLSEAKTLTE QIAMLLWETIIETSDTTL DYLYQHI SVCGS KLTE++LS+LPY
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVDYLYQHIHSVCGSEKLTEDDLSKLPY 360

Query: 361 LTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEW 420
           LTAVFHETLRKHSPVP+VPLRY HED QLGGFFVPAGSEIAVNIYACNMDKD WESPEEW
Sbjct: 361 LTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEW 420

Query: 421 KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREGEEDK 480
           KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IG++VQEFEW+L EGEEDK
Sbjct: 421 KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDK 480

Query: 481 VDTLGLTARKLQPLHLVVKPRIN 488
           VDTLGLTARKLQPLH +VKPRI+
Sbjct: 481 VDTLGLTARKLQPLHAIVKPRIS 502

[Close]

BLAST of HG10014088 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 849.0 bits (2192), Expect = 2.1e-242
Identity = 424/519 (81.70%), Postives = 464/519 (89.40%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MA+  DP+ S+Q L++TLQAPPYAAAA+LG +S +L+ FLVAD +K RRNFLPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           +P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTG+STVIVLNTAEVAKEAMVTRYAS+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
            RKLSKALTILT+DKCMVAMSDYNDFHKM               K+HRQRRD+MI+NI+ 
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           +LFAH+KD PSE+VNFRKIFESELF LALKETLGKDIESIYVDGLGTTLPREDLFRVLVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           D MEGAIEVDWRDFFPYL+WIPNK+VEN+IR+MD+RRR+TM SLIEEQKKRIAAGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTE QIAMLLWETIIETSDTTL                 DYLYQHI SV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTE++LS+LPYLTAVFHETLRKHSPVP+VPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKD WESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           +VQEFEW+L EGEEDKVDTLGLTARKLQPLH++VKPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519

[Close]

BLAST of HG10014088 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.6e-173
Identity = 304/508 (59.84%), Postives = 381/508 (75.00%), Query Frame = 0


Query: 17  TLQAPPYAAA-----AVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLK 76
           +LQA P AAA       +GG++L      V D +KK  +F PP P +PG+P++GNLLQLK
Sbjct: 6   SLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSSF-PPPPKVPGLPVIGNLLQLK 65

Query: 77  EKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTIL 136
           EKKPH TF +W+E YGP+YSIRTG++T+IVLNT +VAKEAM+T+Y+SIS+RKLSKALTIL
Sbjct: 66  EKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMITKYSSISTRKLSKALTIL 125

Query: 137 TADKCMVAMSDYNDFHK---------------MKKHRQRRDTMIENITRELFAHLKDFPS 196
           T+DK +VAMSDYN+F+K                K+HR  R+ MI NI  +  AH K +PS
Sbjct: 126 TSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMYPS 185

Query: 197 ETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDW 256
           E VNFRKIF+SELF L++K+ +G+D+ESIYV+ L TTL R+++F++LV+DPMEGAI+VDW
Sbjct: 186 EAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDVDW 245

Query: 257 RDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNCYADFLLSE-AK 316
           RDFFPYL+WIPN++ EN+I++M F R   MK+LIE+QKKRIA+G+  NCY D LLSE A 
Sbjct: 246 RDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEAAD 305

Query: 317 TLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSVCGSAKLTEEN 376
           TL+E QI MLLWE IIE SDTTL                 +YL   I + CG  +L EE 
Sbjct: 306 TLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLNEEK 365

Query: 377 LSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHW 436
           L +LPYL A+F ETLRKHSPVPVVPLRY HE+TQLGG+ +P GSEIA+NIY CNMDK+ W
Sbjct: 366 LCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIPEGSEIAINIYGCNMDKNVW 425

Query: 437 ESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELR 487
           +SPEEW+PERF+  K D  +LHKTMAFGGGKRVCAGAL+AM I+C  IG++VQE EW L 
Sbjct: 426 DSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAMTISCIAIGRLVQELEWRLG 485

[Close]

BLAST of HG10014088 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-161
Identity = 281/467 (60.17%), Postives = 353/467 (75.59%), Query Frame = 0


Query: 52  LPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEA 111
           LP VP +PG P++GNLLQLKEKKPH TF RW+E YGP+YSI+ GSS++IVLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 MVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRR 171
           MVTR++SIS+RKLS ALT+LT DK MVA SDY+DFHK+               K+ R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPR 231
           D +IEN++ +L AH +D P E VNFR IFE ELF +ALK+  GKD+ESIYV  LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKR 291
           +++F+VLV D MEGAI+VDWRDFFPYL+WIPNK+ E RI++   RR   M +LI+++ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNCYADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------D 351
             +  + +CY +FL+SEAKTLT++QIA+L+WETIIET+DTTL                 D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHILSVCGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVP 411
            L + I +VCG  K  EE LSQ+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYACNMDKDHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIY CNMDK  WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 MLIACTTIGKMVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPR 486
            L+A   IG++VQEFEW+LR+GEE+ VDT GLT++KL PL  ++ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

[Close]

BLAST of HG10014088 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.3e-143
Identity = 256/495 (51.72%), Postives = 339/495 (68.48%), Query Frame = 0


Query: 24  AAAAVLGGVSLMLSVFLVADCKKKRRNFLPP------VPAIPGVPLLGNLLQLKEKKPHM 83
           AAAA +GG       F+ A    +R   + P       PA+PG+P++GNL QLKEKKPH 
Sbjct: 11  AAAAAVGG-------FVAAAALAERAGVIAPRKRPNAPPAVPGLPIIGNLHQLKEKKPHQ 70

Query: 84  TFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCM 143
           TFA+WAE YGP+Y+IRTG+S+V+VLN+ EVAKEAMV +++SIS+RKLSKALT+LT DK M
Sbjct: 71  TFAKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSM 130

Query: 144 VAMSDYNDFHKM---------------KKHRQRRDTMIENITRELFAHLKDFPSETVNFR 203
           VA SDY DFHKM               K+ R  RD MI N+       +KD P   + FR
Sbjct: 131 VATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFR 190

Query: 204 KIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPY 263
            +F+ ELFRL++ ++LG+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPY
Sbjct: 191 DVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPY 250

Query: 264 LRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEDQI 323
           L W+PNK+ E R+   + RR   M++LI++QK+RI  GE   CY DFLL+E  TLT++Q+
Sbjct: 251 LSWVPNKSFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAE-NTLTDEQL 310

Query: 324 AMLLWETIIETSDTTL-----------------DYLYQHILSVCGSAKLTEENLSQLPYL 383
            ML+WE +IE +DTTL                 + LYQ I  VCG   +TEE+L +LPYL
Sbjct: 311 MMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYL 370

Query: 384 TAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWK 443
            AVFHETLR+HSPVP++P R+ HEDT+L G+ VPAG+E+ +N+Y CNM++  WESPEEW 
Sbjct: 371 NAVFHETLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWV 430

Query: 444 PERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREGEEDKV 481
           PERF   + +  D++KTMAFG G+R CAG+L+A  IAC  + + VQEF W LREG+E+KV
Sbjct: 431 PERFAGGRLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKV 490

[Close]

BLAST of HG10014088 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 8.7e-143
Identity = 256/493 (51.93%), Postives = 338/493 (68.56%), Query Frame = 0


Query: 25  AAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAE 84
           AAA +GG   + +  L     K RRN   P PA+PG+P++GNL QLKEKKPH TF +WAE
Sbjct: 15  AAAAVGG--FIAAATLAVAPPKNRRN---PPPAVPGLPIIGNLHQLKEKKPHQTFTKWAE 74

Query: 85  TYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYN 144
            YGP+Y+IRTG+S+V+VLN+ EVAKEAMV +++SIS+RKLSKALT+L+ DK MVA SD  
Sbjct: 75  IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDSG 134

Query: 145 DFHKM---------------KKHRQRRDTMIENITRELFAHLKDFPSETVNFRKIFESEL 204
           DFHKM               K+ R  RD +I N+       +KD P   + FR +F+ EL
Sbjct: 135 DFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDEL 194

Query: 205 FRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNK 264
           FRL++ ++LG+D+ S+YVD  G  + +E+++   V D M  AIEVDWRDFFPYL W+PNK
Sbjct: 195 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 254

Query: 265 NVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEDQIAMLLWET 324
           + E R+   + RR   M++LI++QK+RI  GE   CY DFLL+E  TLT++Q+ ML+WE 
Sbjct: 255 SFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAE-NTLTDEQLMMLVWEA 314

Query: 325 IIETSDTTL-----------------DYLYQHILSVCGSAKLTEENLSQLPYLTAVFHET 384
           +IE +DTTL                 + LYQ I  VCG   +TEE+L  LPYL AVF ET
Sbjct: 315 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQET 374

Query: 385 LRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDE 444
           LR+HSPVP++P R+ +EDT L G+ VPAG+E+ +N+Y CNM+K  WESPEEW PERF   
Sbjct: 375 LRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAGG 434

Query: 445 KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREGEEDKVDTLGLTA 486
           ++   D++KTMAFG G+RVCAG+L+A  IAC  I + VQEF W LREG+E+KVDT+ LTA
Sbjct: 435 RFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLTA 494

[Close]

BLAST of HG10014088 vs. ExPASy Swiss-Prot
Match: Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.2e-130
Identity = 239/498 (47.99%), Postives = 336/498 (67.47%), Query Frame = 0


Query: 24  AAAAVLGGV--SLMLSVFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFAR 83
           AA A +GG+  +  L+  LVA     R+N   P PA+PG+P++GNL QLKEKKPH TFA+
Sbjct: 12  AAVAAVGGLVAAAALADKLVA-APPPRKNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAK 71

Query: 84  WAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMS 143
           W+ETYGP+Y+I+TG+S V+VLN+ EVAKEAM+ +++SIS+RKL KA+++LT  K MVA+S
Sbjct: 72  WSETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKFSSISTRKLPKAMSVLTR-KSMVAIS 131

Query: 144 DYNDFHKM---------------KKHRQRRDTMIENITRELFAHLKDFPSETVNFRKIFE 203
           DY D+ KM               K+ R  R+ MI N+   L   +   P   +NFR ++ 
Sbjct: 132 DYGDYQKMAKRNIMIGMLGFNAQKQFRGTRERMISNVLSTLHKLVSLDPHSPLNFRDVYI 191

Query: 204 SELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWI 263
           +ELF L+L ++LG+D+ S+YV+  G  + ++++F VLV + M  A+E DWRD+FPYL W+
Sbjct: 192 NELFSLSLIQSLGEDVSSVYVEEFGREISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWL 251

Query: 264 PNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNCYADFLLSEAKT--LTEDQIAM 323
           PNK+ +  +   +FRR   M +LI++QK+RIA GE    Y DFLL   ++  LT+DQ+ +
Sbjct: 252 PNKSFDTIVSTTEFRRDAIMNALIKKQKERIARGEARASYIDFLLEAERSAQLTDDQLML 311

Query: 324 LLWETIIETSDTTL-----------------DYLYQHILSVCGSAKLTEENLSQLPYLTA 383
           LL E+I+  +DT L                 + LYQ I   CG   +TE++L +LPYL A
Sbjct: 312 LLSESILAAADTVLVTTEWTMYEIAKNPDKQELLYQEIREACGGEAVTEDDLPRLPYLNA 371

Query: 384 VFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPE 443
           VFHETLR HSPVPV+P R+ H+DT L G+ + AG+++ +N+YAC+MD+  WESP EW PE
Sbjct: 372 VFHETLRLHSPVPVLPPRFVHDDTTLAGYDIAAGTQMMINVYACHMDEKVWESPGEWSPE 431

Query: 444 RFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREGEEDKVDT 486
           RFL E ++  D +KTMAFG G+R CAG+L+AM IAC  + ++VQE EW LREG+ DK DT
Sbjct: 432 RFLGEGFEVADRYKTMAFGAGRRTCAGSLQAMNIACVAVARLVQELEWRLREGDGDKEDT 491

[Close]

BLAST of HG10014088 vs. ExPASy TrEMBL
Match: A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.5e-257
Identity = 449/519 (86.51%), Postives = 475/519 (91.52%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ASMQLL NTL APPYAAAAVLGGVSL+LSVFLVADC+KKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTG+STVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKM               KKHRQRRD MIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FPS+TVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK+VEN+IR MDFRRRMTMKSLI+E K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDK+HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           MVQEFEW+LREGEE+KVDTLGLTARKLQPLH+V+KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of HG10014088 vs. ExPASy TrEMBL
Match: A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 5.5e-257
Identity = 449/519 (86.51%), Postives = 475/519 (91.52%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ASMQLL NTL APPYAAAAVLGGVSL+LSVFLVADC+KKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTG+STVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKM               KKHRQRRD MIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FPS+TVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK+VEN+IR MDFRRRMTMKSLI+E K+RIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDK+HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           MVQEFEW+LREGEE+KVDTLGLTARKLQPLH+V+KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of HG10014088 vs. ExPASy TrEMBL
Match: J7I3T1 (Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.6e-254
Identity = 444/519 (85.55%), Postives = 469/519 (90.37%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MAVVTDP+ASMQLL NT+ APPYAAAAVLGGVSL+LSVF VADC+KKRRNFLPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVPAVPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           VP+LGNLLQLKEKKPH TFARWAETYG VYSIRTG+STVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILTADKCMVAMSDYN+FHKM               KKHRQRRD MIENI+R
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           ELFAH+K+FP +TVNFRKIFE+ELFRLALKETLGKDIESIYVDGLGTTLPREDLFR+LVI
Sbjct: 181 ELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYLRWIPNK VEN+IR MDFRRRMTMK L+EE KKRIAAGEET C
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTEDQI+MLLWETIIETSDTTL                 DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGSA LTEENLSQLPYLTA+FHETLRKHSPVPVVPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKDHWESPEEWKPERFLD+KYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIG+
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           MVQEFEW+LREGEE+KVDTLGLTARKLQPLH+V+KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of HG10014088 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.0e-242
Identity = 424/519 (81.70%), Postives = 464/519 (89.40%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           MA+  DP+ S+Q L++TLQAPPYAAAA+LG +S +L+ FLVAD +K RRNFLPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           +P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTG+STVIVLNTAEVAKEAMVTRYAS+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
            RKLSKALTILT+DKCMVAMSDYNDFHKM               K+HRQRRD+MI+NI+ 
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           +LFAH+KD PSE+VNFRKIFESELF LALKETLGKDIESIYVDGLGTTLPREDLFRVLVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           D MEGAIEVDWRDFFPYL+WIPNK+VEN+IR+MD+RRR+TM SLIEEQKKRIAAGEETNC
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTE QIAMLLWETIIETSDTTL                 DYLYQHI SV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTE++LS+LPYLTAVFHETLRKHSPVP+VPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKD WESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           +VQEFEW+L EGEEDKVDTLGLTARKLQPLH++VKPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519

[Close]

BLAST of HG10014088 vs. ExPASy TrEMBL
Match: A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 422/519 (81.31%), Postives = 462/519 (89.02%), Query Frame = 0


Query: 1   MAVVTDPIASMQLLVNTLQAPPYAAAAVLGGVSLMLSVFLVADCKKKRRNFLPPVPAIPG 60
           M +  DP+ S+Q L++TLQAP YAAA++LG +S +L+ FLVAD +K RRNFLPPVPAIPG
Sbjct: 1   MVIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASIS 120
           +P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTG+STVIVLNTAEVAKEAMVTRYAS+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRRDTMIENITR 180
           SRKLSKALTILT+DKCMVAMSDYNDFHKM               K+HRQRRDTMI+NI+ 
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 ELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240
           +LFAH+KD PSE+VNFR+IFESELFRLALKETLGKDIESIYVDGLG  LPREDLFRVLVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-MLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKRIAAGEETNC 300
           DPMEGAIEVDWRDFFPYL+WIPNK+VEN+IR+MDFRRR+TM SLIE+QKKRIAAGEETNC
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTMMSLIEKQKKRIAAGEETNC 300

Query: 301 YADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------DYLYQHILSV 360
           YADFLLSEAKTLTE QIAMLLWETIIETSDTTL                 DYLYQHI SV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPKRQDYLYQHIQSV 360

Query: 361 CGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KLTE++LS+LPYLTAVFHETLRKHSPVP+VPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGK 480
           YACNMDKD WESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+IG+
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 MVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPRIN 488
           +VQEFEW+L EGEEDKVDTLGLTARKLQPLH +VKPRI+
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPRIS 518

[Close]

BLAST of HG10014088 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 571.2 bits (1471), Expect = 7.7e-163
Identity = 281/467 (60.17%), Postives = 353/467 (75.59%), Query Frame = 0


Query: 52  LPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTAEVAKEA 111
           LP VP +PG P++GNLLQLKEKKPH TF RW+E YGP+YSI+ GSS++IVLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 MVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKM---------------KKHRQRR 171
           MVTR++SIS+RKLS ALT+LT DK MVA SDY+DFHK+               K+ R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPR 231
           D +IEN++ +L AH +D P E VNFR IFE ELF +ALK+  GKD+ESIYV  LG TL +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTMKSLIEEQKKR 291
           +++F+VLV D MEGAI+VDWRDFFPYL+WIPNK+ E RI++   RR   M +LI+++ K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNCYADFLLSEAKTLTEDQIAMLLWETIIETSDTTL-----------------D 351
             +  + +CY +FL+SEAKTLT++QIA+L+WETIIET+DTTL                 D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHILSVCGSAKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVP 411
            L + I +VCG  K  EE LSQ+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYACNMDKDHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIY CNMDK  WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 MLIACTTIGKMVQEFEWELREGEEDKVDTLGLTARKLQPLHLVVKPR 486
            L+A   IG++VQEFEW+LR+GEE+ VDT GLT++KL PL  ++ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

[Close]

BLAST of HG10014088 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-36
Identity = 128/495 (25.86%), Postives = 220/495 (44.44%), Query Frame = 0


Query: 38  VFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSS 97
           +F      +  R  +P  P  P +P++GN + L  + PH +FA  ++TYGP+ S++ GS 
Sbjct: 22  IFFTTTRPRSSRKVVPSPPGPPRLPIIGN-IHLVGRNPHHSFADLSKTYGPIMSLKFGSL 81

Query: 98  TVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMV-----AMSDYNDFHKMK-- 157
             +V+ + E A+E + T    +SSR  + ++  +  DK  V     + S +    K+   
Sbjct: 82  NTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSSRWRLLRKLSAT 141

Query: 158 -----KHRQRRDTMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESI 217
                +  +   T+ EN  +EL +    F SE+    +    ++ R      L      +
Sbjct: 142 QLFSPQRIEATKTLRENKVKELVS----FMSESSEREEAV--DISRATFITALNIISNIL 201

Query: 218 YVDGLGT-TLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFR--- 277
           +   LG     +  +F+  VI  ME     D  +FFP+L ++  +     ++    R   
Sbjct: 202 FSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFK 261

Query: 278 -------RRMTMKSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEDQIAMLLWETI---I 337
                   ++  KSL +   K +   +  +   D    +   L  + I  LL +      
Sbjct: 262 VFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLLLDLFGAGT 321

Query: 338 ETSDTTLDYLYQHIL--------------SVCGSAKLTEE-NLSQLPYLTAVFHETLRKH 397
           +T+ +T+++    +L               V G   + EE ++S LPYL AV  ET R H
Sbjct: 322 DTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVVKETFRLH 381

Query: 398 SPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDEKYDP 457
              P++  R A  D ++ GF VP  +++ VN++A   D + WE+   +KPERFL +  D 
Sbjct: 382 PAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERFLGKDIDL 441

Query: 458 MDL-HKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREG----EEDKVDTLGLT 486
               ++   FG G+R+C G   A+      +  ++  F+W+L  G    + D  +T GLT
Sbjct: 442 RGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDMDETFGLT 501

[Close]

BLAST of HG10014088 vs. TAIR 10
Match: AT4G13770.1 (cytochrome P450, family 83, subfamily A, polypeptide 1 )

HSP 1 Score: 149.1 bits (375), Expect = 9.5e-36
Identity = 128/507 (25.25%), Postives = 240/507 (47.34%), Query Frame = 0


Query: 28  VLGGVSL-MLSVFLVADCKKKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETY 87
           ++G V+L  + +F +    K +R  LPP P+   +P++GNLLQL++  P   FA WA+ Y
Sbjct: 5   IIGVVALAAVLLFFLYQKPKTKRYKLPPGPS--PLPVIGNLLQLQKLNPQRFFAGWAKKY 64

Query: 88  GPVYSIRTGSSTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDF 147
           GP+ S R GS T++V+++AE+AKE + T+  + + R   +    ++  +  +A++ Y  +
Sbjct: 65  GPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPY 124

Query: 148 HKMKKHRQRRDTMIENITRELFAHLKDFPSETVNFRKIFESELFRL------ALKETLGK 207
           ++              I +    HL   P+    F+ + E E  R+      A  ++   
Sbjct: 125 YR-------------EIRKMGMNHLFS-PTRVATFKHVREEEARRMMDKINKAADKSEVV 184

Query: 208 DIESIYVDGLGTTLPR-----------EDLFRVLVIDPMEGAI--EVDWRDFFPYLRWIP 267
           DI  + +    + + R           E++ R + I     ++  ++ + DFFPY  ++ 
Sbjct: 185 DISELMLTFTNSVVCRQAFGKKYNEDGEEMKRFIKILYGTQSVLGKIFFSDFFPYCGFLD 244

Query: 268 NKNVENRIRKMDFRRRMT-MKSLIEEQKKRIAAGEETNCYADFLLS------EAKTLTED 327
           + +      K  F R+ T ++ ++ E         ET    D L+        A   T D
Sbjct: 245 DLSGLTAYMKECFERQDTYIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVD 304

Query: 328 QIAMLLWETIIETSDTT-------LDYL--YQHILSVC-----------GSAKLTEENLS 387
            +  ++ + ++  +DT        + YL  Y  +L              GS  +TE+++ 
Sbjct: 305 NVKAVILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVK 364

Query: 388 QLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHW-E 447
            LPY  A+  ETLR    +P++  R   +DT++ G+ +PAG+ + VN +A + D+  W  
Sbjct: 365 NLPYFRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTVNVNAWAVSRDEKEWGP 424

Query: 448 SPEEWKPERFLDEKYDPMDL-HKTMAFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELR 482
           +P+E++PERFL+++ D     ++ + FG G+R+C G      +       ++  F ++L 
Sbjct: 425 NPDEFRPERFLEKEVDFKGTDYEFIPFGSGRRMCPGMRLGAAMLEVPYANLLLSFNFKLP 484

[Close]

BLAST of HG10014088 vs. TAIR 10
Match: AT1G74550.1 (cytochrome P450, family 98, subfamily A, polypeptide 9 )

HSP 1 Score: 144.8 bits (364), Expect = 1.8e-34
Identity = 121/481 (25.16%), Postives = 220/481 (45.74%), Query Frame = 0


Query: 46  KKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTA 105
           ++R + +PP P  P   L+GNL QLK      +F+ W++TYGP+ S+  GS   +V++++
Sbjct: 21  RRRGSNIPPGP--PTRFLVGNLHQLKPLWT-QSFSEWSQTYGPIISVWLGSQLAVVVSSS 80

Query: 106 EVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYN-DFHKMKK------------- 165
           ++AK+ +  +   + +R  +  +T   +D   +  SDY   + KM+K             
Sbjct: 81  DLAKQVLRDKDYQLCNRHRTARMTQNGSD---LIWSDYGAHYVKMRKLCTLELFSLKSIE 140

Query: 166 -HRQRRDTMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIYVDGL 225
             R  R+  + ++ + +F        + V  R   +S    +  +  +GK  E    DG 
Sbjct: 141 CFRSMREMEVSSMVKSIFNDFMSDDQKPVVLRNYLDSVALNIVSRLVIGKTFEP--KDG- 200

Query: 226 GTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENR--IRKMDFRRRMTMKS 285
                RE  FR +V             D+  +L+ + +    ++  ++ M  +R    ++
Sbjct: 201 -----RE--FRSIVERETRLPGATKMLDYTVWLKRLSSWFTSDKAFMKHMARKRNWFKRA 260

Query: 286 LIEEQKKRIAAGEETNCYADFL--LSEAKTLTEDQIAMLLWETIIETSDTTL-------- 345
           +++E    +  G +  C+   L  L E   LTE+ +  L+W  +   +DTT         
Sbjct: 261 VMDE----VYGGRDQKCFVQSLLELKEKDELTEETVMGLVWNMLTAGADTTAITIEWAMA 320

Query: 346 ---------DYLYQHILSVCGSAKL-TEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAH 405
                    + +   + SV GS +L ++ ++ +LP+L  V  E LR H P P++    A 
Sbjct: 321 EMIRCPTVKEKVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEALRLHPPTPLMLPHKAS 380

Query: 406 EDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDEKYDPMDL-HKTMAFGG 465
           E  Q+GG+ VP G+ + VN+ A   D  +W +P+E++PERFL E+ D      + + FG 
Sbjct: 381 ESVQVGGYKVPKGATVYVNVQAIARDPANWSNPDEFRPERFLVEETDVKGQDFRVLPFGS 440

Query: 466 GKRVCAGALKAMLIACTTIGKMVQEFEWELREGEE--DKVDTLGLTARKLQPLHLVVKPR 487
           G+RVC  A  ++ +    +G ++  F W      E  D  +  GL      PL  +   R
Sbjct: 441 GRRVCPAAQLSLNMMTLALGSLLHCFSWTSSTPREHIDMTEKPGLVCYMKAPLQALASSR 481

[Close]

BLAST of HG10014088 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 144.8 bits (364), Expect = 1.8e-34
Identity = 119/487 (24.44%), Postives = 225/487 (46.20%), Query Frame = 0


Query: 46  KKRRNFLPPVPAIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGSSTVIVLNTA 105
           ++ R   PP P+    P++GNL  +K  +    +  WA++YGP+ S+  GS   +V+++A
Sbjct: 21  QRLRYKFPPGPS--PKPIVGNLYDIKPVR-FRCYYEWAQSYGPIISVWIGSILNVVVSSA 80

Query: 106 EVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFH---------------KMK 165
           E+AKE +      ++ R  +++    + +   +  +DY   +               +++
Sbjct: 81  ELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLE 140

Query: 166 KHRQRRD----TMIENITRELFAHLKDFPSETVNFRKIFESELFRLALKETLGKDIESIY 225
             R  R+     M+E++ R+   +L +  ++ +  RK   +  F    +   GK     +
Sbjct: 141 SLRPIREDEVTAMVESVFRD--CNLPENRAKGLQLRKYLGAVAFNNITRLAFGKR----F 200

Query: 226 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKNVENRIRKMDFRRRMTM 285
           ++  G    +   F+ +V + ++    +   +  P+LRW+   + E    +   RR    
Sbjct: 201 MNAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWMFPAD-EKAFAEHGARRDRLT 260

Query: 286 KSLIEEQ---KKRIAAGEETNCYADFLLSEAKTLTEDQIAMLLWETIIETSDTTL----- 345
           ++++EE    +++ +  ++    A   L +   L+ED I  LLW+ I    DTT      
Sbjct: 261 RAIMEEHTLARQKSSGAKQHFVDALLTLKDQYDLSEDTIIGLLWDMITAGMDTTAITAEW 320

Query: 346 ------------DYLYQHILSVCGSAK-LTEENLSQLPYLTAVFHETLRKHSPVPVVPLR 405
                         + +    V G  + LTE + S+LPYL  V  E+ R H P P++   
Sbjct: 321 AMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPH 380

Query: 406 YAHEDTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDEKYDPMDLH--KTM 465
            ++ D ++GG+ +P GS + VN++A   D   W++P E++PERFL+E  D M  H  + +
Sbjct: 381 RSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVD-MKGHDFRLL 440

Query: 466 AFGGGKRVCAGALKAMLIACTTIGKMVQEFEWELREG----EEDKVDTLGLTARKLQPLH 487
            FG G+RVC GA   + +  + +  ++  F W   +G    E D  +  GL      P+ 
Sbjct: 441 PFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQ 496

The following BLAST results are available for this feature:

BLAST of HG10014088 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898390.1	4.7e-263	89.02	ent-kaurene oxidase [Benincasa hispida]	[more]
XP_008452488.1	1.1e-256	86.51	PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-k...	[more]
NP_001267703.1	2.0e-253	85.55	ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kauren...	[more]
KAG7024228.1	1.2e-242	84.29	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022976063.1	2.1e-242	81.70	ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of HG10014088 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0G2RKY1	3.6e-173	59.84	Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1	[more]
Q93ZB2	1.1e-161	60.17	Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV...	[more]
Q5Z5R4	2.3e-143	51.72	Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ...	[more]
Q5Z5R7	8.7e-143	51.93	Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19...	[more]
Q0DBF4	2.2e-130	47.99	Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of HG10014088 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D9I5	5.5e-257	86.51	Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G...	[more]
A0A1S3BTZ0	5.5e-257	86.51	ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 ...	[more]
J7I3T1	9.6e-254	85.55	Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1	[more]
A0A6J1IL06	1.0e-242	81.70	ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]
A0A6J1F7C8	1.6e-240	81.31	ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of HG10014088 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25900.1	7.7e-163	60.17	GA requiring 3	[more]
AT2G45570.1	1.5e-36	25.86	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT4G13770.1	9.5e-36	25.25	cytochrome P450, family 83, subfamily A, polypeptide 1	[more]
AT1G74550.1	1.8e-34	25.16	cytochrome P450, family 98, subfamily A, polypeptide 9	[more]
AT2G40890.1	1.8e-34	24.44	cytochrome P450, family 98, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 107..128 score: 21.43 coord: 346..364 score: 32.3 coord: 422..432 score: 51.84 coord: 83..102 score: 36.12 coord: 387..411 score: 35.54 coord: 432..455 score: 22.59
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 45..487 e-value: 1.8E-81 score: 276.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 53..485
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 321..465 e-value: 1.8E-38 score: 132.5 coord: 59..317 e-value: 6.2E-16 score: 58.2
IPR044225	Ent-kaurene oxidase, chloroplastic	PANTHER	PTHR47283	ENT-KAURENE OXIDASE, CHLOROPLASTIC	coord: 24..485
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 425..434

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014088.1	HG10014088.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010241	ent-kaurene oxidation to kaurenoic acid
biological_process	GO:0009686	gibberellin biosynthetic process
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0052615	ent-kaurene oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen