HG10014075 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014075
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	cytochrome P450 82A3-like
Location	Chr02: 7364060 .. 7366076 (-)
RNA-Seq Expression	HG10014075
Synteny	HG10014075

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAAAATAAGCATCTCCGAGCTTCTCTCAAACCAACGTGTCGACATGCTTAAGCCCGTCATGTTATCTCAAGTCGACATGTCATTGAATGAGCTACACGACATGTCGTTAAATGGGGCAGGCCGGGTTTCGGTGGAGATGAGGACGTGGTTTGGGAATGTGGCTTTGAATACAGTTTTGAAGGTGATAATGGGAAAACGATGTGTCGGTCCAAACGGTGAAGGTTGTGAAGAGGTGCGGCGAAAGAGCTTCAGAAGACGATTACGGATTCGTTTCGTATTATGGATGGAGGGTTGTTGAGTGATTATTTTCCTTTATTTGGATGGTTGGGAATTGATGGGGAAGTTAAGAAGATTGAGAAAATAGCGAAACGATCTGATCGGATTCTTCAACGTTGGCTGGAAGAACACAAGAGGAAGAGATCGGGTTGTGGAAGTTTTACTAAATCCGGAATCAATGGTTCCGAGACCGTAGCGATCATCTCTTGATACTCGGGAGATCCCATGCGAGAGGGGCTTGGTAGCTAAGAAAGTCAAGTCAGGGAGGAAGGCGCTGTACTATTGGGCAAGTAGCGGTTGTAGTCTCGTCTTTCTTGTAGCTTTGGGAATTTTTTTATTGGATACAAAAGAAAATGGAGATTTTATGGATTCTCTTCTTTTCTTACTTGATCGTAACAAGCTTCCTACTTGCTTCGATGGTGATACCATTATCAAAGCCACGATGCTGGTACCCTTTTTTCTTTGAACCATAAATTTCTTGAATGAAAATTGAGACATGAAGTAGTGTGTCGATAATCTGAAAATGAAAACTGAGACATGACGAATATCACATATAGGTCATCCGCCACACTCATTTTAAGTTCTAATTTTCTTCTAATGTTGATTAGTTTTGAAAACTTTATAGATGGCTGATAGCTTTTGAAATAATATGAGTGTTTTTTTTTATAAAAAAGAATAAATGGTAAAGTTAAAGGTATTTTTTTTTTCTAAAAAAAAAAATTTTAAGGTATTCTTTTGTTACGAAAAATACTTAAGTGCGGATTTTAGGCTGCAACTATCTTTGTATAGTTTATCTAGTTGTCTAATTAAAAATTGTCACATGGCGAACATTTTTTTGTTAAAAAGAGAATTGAATTGTTTTTTTTACTATGTAATAAAAGATGGTGGCACCTAATCATTATTTTTTTTTTGTTATTAGCATGTTTTCAATTAGGACCAACACCTAAGTATATTTTTTTTTTAAAAAAAAAAGTGTTAGATGATATAATATTAAATTTGTCTTTACCCATCAACTTAAGCTTTTGAGTCAATCGGTGATTTAAGATGGTATCAGAACAATGTTATTTCCTTCCTAATTAATATTGATTTCTACTTGTTGGGTGTATGTGTAGAGTATGATTTCAGGTGGAACTGAAAGCACGACGGTGACCTTAACATGGGCAATATCACTAATGCTAAACAACCCACATACCATAGAGAGGGCACAACAAGAGTTGGACGAAATAGTTGGGAGAGAAAGACGAGTGAATGAGTCAGACATAAGCAAGCTTGTTTACATTCAAGCCATAATTAAAGAGACAATGAGATTATACCCAGCAACCCCATTGGTAGGACCACAGGAGTTCCTTGAAGACTGCATGGTGGGAGACTACTTTGTCCCAAAAGGAACACGGTTGATTCAAAACGTCTGAAAGATCCAAATTGACCATGTGATTTGGCCTAACCCATTCGAGTTCAAGCCGGAGCGGTTTTTGACGACACACAAGGATGTTGAACTCAAAGGGAAGAACTTTGAGTTGCTTCCTTTTGGAACTGGGCGAAGAGGATGTCCTGGAGTTGCATTTGCGCTTCAAGTTGAACATTTCACTTTGGTTGGGTTTCTGCACTTGTTTCATGTCAAAAATTCGACGCCTGAATTGATTGATATGTCGGAAAGGTATGGATTGGCGAACGAGAAAGTGACGCCTCTCCATGTTTTGGTATCTCCTAGGTTGCCTTTACATCTTTACGCCTAA

mRNA sequence

ATGCGTAAAATAAGCATCTCCGAGCTTCTCTCAAACCAACGTGTCGACATGCTTAAGCCCGTCATGTTATCTCAAGTCGACATGTCATTGAATGAGCTACACGACATGTCGTTAAATGGGGCAGGCCGGGTTTCGGTGGAGATGAGGACGTGGTTTGGGAATGTGGCTTTGAATACAGTTTTGAAGGTGATAATGGGAAAACGATGTGTCGGTCCAAACGGTGAAGGTTGTGAAGAGGTGCGGCGAAAGAGCTTCAGAAGACGATTACGGATTCGTTTCGTATTATGGATGGAGGCTAAGAAAGTCAAGTCAGGGAGGAAGGCGCTGTACTATTGGGCAAGTAGCGGTTGTAGTCTCGTCTTTCTTGTAGCTTTGGGAATTTTTTTATTGGATACAAAAGAAAATGGAGATTTTATGGATTCTCTTCTTTTCTTACTTGATCGTAACAAGCTTCCTACTTGCTTCGATGGTGATACCATTATCAAAGCCACGATGCTGAGTATGATTTCAGGTGGAACTGAAAGCACGACGGTGACCTTAACATGGGCAATATCACTAATGCTAAACAACCCACATACCATAGAGAGGGCACAACAAGAGTTGGACGAAATAGTTGGGAGAGAAAGACGAGTGAATGAGTCAGACATAAGCAAGCTTGTTTACATTCAAGCCATAATTAAAGAGACAATGAGATTATACCCAGCAACCCCATTGATCCAAATTGACCATGTGATTTGGCCTAACCCATTCGAGTTCAAGCCGGAGCGGTTTTTGACGACACACAAGGATGTTGAACTCAAAGGGAAGAACTTTGAGTTGCTTCCTTTTGGAACTGGGCGAAGAGGATGTCCTGGAGTTGCATTTGCGCTTCAAGTTGAACATTTCACTTTGGTTGGGTTTCTGCACTTGTTTCATGTCAAAAATTCGACGCCTGAATTGATTGATATGTCGGAAAGGTATGGATTGGCGAACGAGAAAGTGACGCCTCTCCATGTTTTGGTATCTCCTAGGTTGCCTTTACATCTTTACGCCTAA

Coding sequence (CDS)

ATGCGTAAAATAAGCATCTCCGAGCTTCTCTCAAACCAACGTGTCGACATGCTTAAGCCCGTCATGTTATCTCAAGTCGACATGTCATTGAATGAGCTACACGACATGTCGTTAAATGGGGCAGGCCGGGTTTCGGTGGAGATGAGGACGTGGTTTGGGAATGTGGCTTTGAATACAGTTTTGAAGGTGATAATGGGAAAACGATGTGTCGGTCCAAACGGTGAAGGTTGTGAAGAGGTGCGGCGAAAGAGCTTCAGAAGACGATTACGGATTCGTTTCGTATTATGGATGGAGGCTAAGAAAGTCAAGTCAGGGAGGAAGGCGCTGTACTATTGGGCAAGTAGCGGTTGTAGTCTCGTCTTTCTTGTAGCTTTGGGAATTTTTTTATTGGATACAAAAGAAAATGGAGATTTTATGGATTCTCTTCTTTTCTTACTTGATCGTAACAAGCTTCCTACTTGCTTCGATGGTGATACCATTATCAAAGCCACGATGCTGAGTATGATTTCAGGTGGAACTGAAAGCACGACGGTGACCTTAACATGGGCAATATCACTAATGCTAAACAACCCACATACCATAGAGAGGGCACAACAAGAGTTGGACGAAATAGTTGGGAGAGAAAGACGAGTGAATGAGTCAGACATAAGCAAGCTTGTTTACATTCAAGCCATAATTAAAGAGACAATGAGATTATACCCAGCAACCCCATTGATCCAAATTGACCATGTGATTTGGCCTAACCCATTCGAGTTCAAGCCGGAGCGGTTTTTGACGACACACAAGGATGTTGAACTCAAAGGGAAGAACTTTGAGTTGCTTCCTTTTGGAACTGGGCGAAGAGGATGTCCTGGAGTTGCATTTGCGCTTCAAGTTGAACATTTCACTTTGGTTGGGTTTCTGCACTTGTTTCATGTCAAAAATTCGACGCCTGAATTGATTGATATGTCGGAAAGGTATGGATTGGCGAACGAGAAAGTGACGCCTCTCCATGTTTTGGTATCTCCTAGGTTGCCTTTACATCTTTACGCCTAA

Protein sequence

MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGRVSVEMRTWFGNVALNTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYWASSGCSLVFLVALGIFLLDTKENGDFMDSLLFLLDRNKLPTCFDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVYIQAIIKETMRLYPATPLIQIDHVIWPNPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPELIDMSERYGLANEKVTPLHVLVSPRLPLHLYA

Homology

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BLAST of HG10014075 vs. NCBI nr
Match: XP_038897329.1 (cytochrome P450 82A3-like [Benincasa hispida])

HSP 1 Score: 466.5 bits (1199), Expect = 2.0e-127
Identity = 263/403 (65.26%), Postives = 287/403 (71.22%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMS--LNGAGRVSVEMRTWFGNVALN 60
           MRKI IS LLSNQRVDMLK VM SQ++MSLNEL+D +   N +G VSVEMR WF +VALN
Sbjct: 140 MRKIIISHLLSNQRVDMLKTVMFSQIEMSLNELYDAARRSNESGGVSVEMRRWFRDVALN 199

Query: 61  TVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVL--------------WM----EAK 120
           TVLK+IMGKRCVGPN  GC+E   K  ++ +   F +              W+    + K
Sbjct: 200 TVLKIIMGKRCVGPN--GCDEDAAKELQKTITESFRIMDGGLLSDFFPLFGWLGLDRDVK 259

Query: 121 KV----KSGRKALYYWAS------SGCSLVFLVALGIFLLDTKENGDFMDSLLFLLDRNK 180
           K+    K   + L  W        SGC        G   LDTKENGDFMDSL+ L DRNK
Sbjct: 260 KIEKIAKRSDRVLQRWLEEHRRKRSGC--------GSLSLDTKENGDFMDSLITLHDRNK 319

Query: 181 LPTCFDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERR 240
           LPTCFDGDTIIKAT+LSMISGGTEST VTLTWAISLMLNNPHTIE+AQQELDEIVGRERR
Sbjct: 320 LPTCFDGDTIIKATILSMISGGTESTMVTLTWAISLMLNNPHTIEKAQQELDEIVGRERR 379

Query: 241 VNESDISKLVYIQAIIKETMRLYPATPL-----------------------------IQI 300
           VNESDI+KLVYIQAIIKETMRLYP  PL                             IQ 
Sbjct: 380 VNESDINKLVYIQAIIKETMRLYPTAPLIGPREFLQDCMVGDYFVSKGTQLIPNVWKIQT 439

Query: 301 DHVIWPNPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFL 345
           D  IWP+PFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQ+E F L GFL
Sbjct: 440 DPKIWPDPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQMEQFILAGFL 499

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BLAST of HG10014075 vs. NCBI nr
Match: XP_022154118.1 (cytochrome P450 82A3-like [Momordica charantia])

HSP 1 Score: 361.7 bits (927), Expect = 7.1e-96
Identity = 203/388 (52.32%), Postives = 255/388 (65.72%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGRVSVEMRTWFGNVALNTV 60
           MRKI+  ELLSN RVD +K +M +Q+DMSL ELH     G+ ++ VEM+ WFG+V +N +
Sbjct: 182 MRKITHLELLSNSRVDQVKNLMFAQIDMSLAELH----AGSAQIPVEMKRWFGDVIVNML 241

Query: 61  LKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVK----SGRKAL--YYWAS 120
           LK+I+GKRCVGPN EG E+   K  +  +R  F L  +          GR          
Sbjct: 242 LKIIIGKRCVGPNAEGGEK-GAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVM 301

Query: 121 SGCSLVFLVALGIFL----------LDTKENGDFMDSLLFLLDRNKLPTCFDGDTIIKAT 180
              +  F V +  +L          L   ++GDFMDSL+ LLD   +   +D DTIIKAT
Sbjct: 302 ESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKAT 361

Query: 181 MLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVYIQA 240
            L+MI+GGTESTTVTLTW ++L+LNNPHT+E AQQELD +VGR+RRVNESDISKL Y+QA
Sbjct: 362 TLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQA 421

Query: 241 IIKETMRLYPATPL-----------------------------IQIDHVIWPNPFEFKPE 300
           ++KE MR+YPA PL                             IQ D  +WPNPFEFKPE
Sbjct: 422 VVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPE 481

Query: 301 RFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPELID 344
           RFLTTHKDV+ KG +FEL+PFG+GRRGCPGVAF LQ+ HFTL GFL  F VK+S+ +LID
Sbjct: 482 RFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLID 541

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BLAST of HG10014075 vs. NCBI nr
Match: XP_008464762.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-95
Identity = 201/393 (51.15%), Postives = 254/393 (64.63%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           +RK++  ELLSN RVD  K  M  +V  SL ELH+      +G+G+V VEM+ WFG+V +
Sbjct: 140 IRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQKDGSGQVEVEMKRWFGDVIV 199

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYW----- 120
           N +LK+I+GKRCVGPN +G E+ + K F+  +R  F L    + +      L  W     
Sbjct: 200 NMLLKIIIGKRCVGPNAKGGEK-QAKDFQLAIRDSFHLM--GQGLLRDYVPLIGWLGFNG 259

Query: 121 ---ASSGCSLVFLVALGIFLLDTKEN----------GDFMDSLLFLLDRNKLPTCFDGDT 180
                   +  F V L  +L + K N          GDFMD+L+ L D   +   +DGDT
Sbjct: 260 QVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALVSLYDGKGIEGYYDGDT 319

Query: 181 IIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKL 240
           IIKAT L+M++GGTESTTVTLTWA+SL+LNNPH +ERAQQELD +VGR+RR+ ESDI  L
Sbjct: 320 IIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDTVVGRDRRLKESDIPNL 379

Query: 241 VYIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPF 300
           VY+Q+IIKETMR+YPA PL                             IQ D  +WP+PF
Sbjct: 380 VYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNLWRIQTDPRVWPDPF 439

Query: 301 EFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNST 344
           +FKPERFLTTHK+V+LKG NFEL+PFG+GRRGCPG AF LQ+ HF L GFLH F V+N T
Sbjct: 440 KFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHFALAGFLHSFDVRNPT 499

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BLAST of HG10014075 vs. NCBI nr
Match: XP_004147824.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_000849 [Cucumis sativus])

HSP 1 Score: 356.3 bits (913), Expect = 3.0e-94
Identity = 196/391 (50.13%), Postives = 258/391 (65.98%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           +RK++  ELLSN RVD  K  M  +V  SL ELH+      +G+G+V VEM+ WFG+V +
Sbjct: 140 IRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIV 199

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLW----------MEAKKVKSGRK 120
           N +LK+I+GKRCVGPN EG E+ + K F+  +R  F L           +  +   +G+ 
Sbjct: 200 NMLLKIIIGKRCVGPNAEGGEK-QAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQV 259

Query: 121 ALYYWASSGCSLVFLVALGIFLLDT------KENGDFMDSLLFLLDRNKLPTCFDGDTII 180
            +    ++   +V    L    L+       +++GDFMD+L+ L D  ++   +DGDTII
Sbjct: 260 KVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIEGYYDGDTII 319

Query: 181 KATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVY 240
           KAT L+M++GGTESTTVTLTWA+SL++NNPH +ERAQQELD +VGR+R++ ESDI  LVY
Sbjct: 320 KATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVY 379

Query: 241 IQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPFEF 300
           +++IIKETMR+YPA PL                             IQ D  +WP+PFEF
Sbjct: 380 LKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEF 439

Query: 301 KPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPE 344
           KPERFLTTHK+V+LKG NFEL+PFG+GRRGCPG+AF LQ+ HF L GFLH F VKN T E
Sbjct: 440 KPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKE 499

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BLAST of HG10014075 vs. NCBI nr
Match: XP_038897047.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 354.0 bits (907), Expect = 1.5e-93
Identity = 201/393 (51.15%), Postives = 255/393 (64.89%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           +RK++  ELLSNQRVD  K  M  ++  SL ELH       + +G+V VEM+ WFG+V L
Sbjct: 140 IRKVTHMELLSNQRVDQFKNTMFGEIKTSLTELHKTWAAQKDESGQVQVEMKRWFGDVIL 199

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYWASSGC 120
           N +LK+I+GKRCVGPN EG E+ + K F+  +R  F L M    ++     +     +G 
Sbjct: 200 NMLLKIIIGKRCVGPNAEGGEK-QAKDFQLAIRDSFHL-MGQGLLRDYIPLIGRLGFNGQ 259

Query: 121 SLV-------FLVALGIFLLD----------TKENGDFMDSLLFLLDRNKLPTCFDGDTI 180
             V       F + L  +L +           K+N DFMD+LL L D  ++   +DGDTI
Sbjct: 260 VKVMENIATRFDMVLREWLEEHKRKRASSSSGKKNEDFMDALLSLYDGKRIEGYYDGDTI 319

Query: 181 IKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLV 240
           IKAT L+MI+GGTESTTVTLTWA+SL+LNNPH +E+AQQELD +VGR+RR+ +SDI  LV
Sbjct: 320 IKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAQQELDTVVGRDRRLKDSDIPNLV 379

Query: 241 YIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPFE 300
           Y+  IIKETMRLYPA PL                             IQ D  +WP+PFE
Sbjct: 380 YLHCIIKETMRLYPAGPLLGPREFYKDCIVDGYFVPKGTQLIPNIWKIQTDPRVWPDPFE 439

Query: 301 FKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTP 345
           FKPERFLTTHK+V+LKG NFEL+PFGTGRRGCPG++F LQ+ HF L GFLH F V+  T 
Sbjct: 440 FKPERFLTTHKNVDLKGNNFELIPFGTGRRGCPGISFGLQMVHFALAGFLHSFDVRKPTK 499

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BLAST of HG10014075 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-65
Identity = 157/419 (37.47%), Postives = 227/419 (54.18%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGR----------------- 60
           +RKI   E+LSN+R+++L  + +S+V  S+ EL ++  N                     
Sbjct: 132 LRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDEP 191

Query: 61  -----VSVEMRTWFGNVALNTVLKVIMGKRCVGP-NGEGCEEVRR-----KSFRRRL--- 120
                VSVE++ WF  + LN VL++++GKRC G  + E  EE +R     + F R +   
Sbjct: 192 STTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGTF 251

Query: 121 ----RIRFVLWMEAKKVKSGRKALYYWASSGCSLVFLVALGIFLLDTKE----------- 180
                + F+ W++    +   K         C+  F V L  +L + +E           
Sbjct: 252 TVGDGVPFLKWLDLGGHEKEMKK--------CAKKFDVMLNEWLEEHREKKGLGSEDKVV 311

Query: 181 -NGDFMDSLLFLLDRNKLPTCFDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHT 240
              DFMD++L +L ++K    FD DTIIKAT L +I GG+++T  TLTWA+ L+L +PH 
Sbjct: 312 GERDFMDAMLLVL-KDKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHV 371

Query: 241 IERAQQELDEIVGRERRVNESDISKLVYIQAIIKETMRLYPATPL--------------- 300
           +E+ ++EL+  +G+ER VNESDI+KLVY+ AIIKET+RLYP  P                
Sbjct: 372 LEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGY 431

Query: 301 --------------IQIDHVIWPNPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCP 344
                         I  D  +WP+P EFKPERFL+THKDV+++G+NFELLPFG+GRR C 
Sbjct: 432 HIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCA 491

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BLAST of HG10014075 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 150/394 (38.07%), Postives = 217/394 (55.08%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGRVS----VEMRTWFGNVA 60
           +RKI   E LSN+R++    + +S+V  S+ EL D+  NG    S    V+++ W   + 
Sbjct: 135 LRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLT 194

Query: 61  LNTVLKVIMGKRCVG-PNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYWASS 120
            N V+++++GKR  G  + EG ++ +R  F + +R  F+  M    V  G   L +    
Sbjct: 195 FNMVVRMVVGKRYFGVMHVEGKDKAQR--FMKNIR-EFMNLMGTFTVADGVPCLRWLDLG 254

Query: 121 GCSLVFLV-------ALGIFLLDTKE----------NGDFMDSLLFLLDRNKLPTCFDGD 180
           G               L  +L + ++          + DFMD ++  L+  ++   FD D
Sbjct: 255 GHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGAQI-GAFDAD 314

Query: 181 TIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISK 240
           TI KAT L +I GGT+ST VTLTWA+SL+L NP  + +A++E+D  +G++  + ESDISK
Sbjct: 315 TICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISK 374

Query: 241 LVYIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNP 300
           LVY+QAI+KET+RLYP  P                              I  D  +W +P
Sbjct: 375 LVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDP 434

Query: 301 FEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNS 344
            EFKPERFLTTHKDV+L+G NFELLPFG+GRR C G++  L + HFTL   LH F + N 
Sbjct: 435 LEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNP 494

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BLAST of HG10014075 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 148/394 (37.56%), Postives = 220/394 (55.84%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGR--VSVEMRTWFGNVALN 60
           MRKI+  ELLSN+R+ MLK V +S++ M + +L+ +     G   V V++++W  ++ LN
Sbjct: 130 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLN 189

Query: 61  TVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRI-RFVLWMEAKKVKSGRKALYYW----- 120
            +++++ GKR  G  G    E   ++ + +  I +F   +    V      L ++     
Sbjct: 190 MIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGH 249

Query: 121 ----ASSGCSLVFLVALGI-------FLLDTKEN-GDFMDSLLFLLDRNKLPTC-FDGDT 180
                 +G  L  ++   I           TKEN  DF+D ++ L ++ KL    +D +T
Sbjct: 250 EKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANT 309

Query: 181 IIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKL 240
            IK+T L++I GG++++  TLTWAISL+LNN   +++AQ E+D  VGR+R V +SDI  L
Sbjct: 310 SIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENL 369

Query: 241 VYIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPF 300
           VY+QAIIKET+RLYPA PL                             IQ D  ++  P 
Sbjct: 370 VYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPN 429

Query: 301 EFKPERFLTTH-KDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNS 344
           EF+PERF+T   K+ +++G+NFEL+PFG+GRR CPG + A+QV H  L  FLH F VK  
Sbjct: 430 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTV 489

[Close]

BLAST of HG10014075 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 229.6 bits (584), Expect = 5.5e-59
Identity = 150/401 (37.41%), Postives = 220/401 (54.86%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDM--SLNGAGRVSVEMRTWFGNVALN 60
           MRKI++ ELLSN+R+ ML  V +S++ M + +L+ +     G+  V V++++W  ++  N
Sbjct: 118 MRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIAN 177

Query: 61  TVLKVIMGKRCVGPNG----EGCEEVR--RKSFRRRLRIRFVL----------WME---- 120
            +++++ GKR  G  G    E  EE R  RK   +   +  +           W++    
Sbjct: 178 MIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGH 237

Query: 121 AKKVKSGRK----ALYYWASSGCSLVFLVALGIFLLDTKEN-GDFMDSLLFLLDRNKLPT 180
            K++K  R+     L  W  +      +         TK N  DF+D +L L ++ KL  
Sbjct: 238 EKEMKQTRRELDVILERWIENHRQQRKVSG-------TKHNDSDFVDVMLSLAEQGKLSH 297

Query: 181 C-FDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVN 240
             +D +T IK T L++I GG+E++  TLTWAISL+LNN   +++ Q E+D  VGR+R V 
Sbjct: 298 LQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVE 357

Query: 241 ESDISKLVYIQAIIKETMRLYPATPL-----------------------------IQIDH 300
           +SDI  LVY+QAIIKET+RLYPA PL                             IQ D 
Sbjct: 358 DSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDP 417

Query: 301 VIWPNPFEFKPERFLTTH-KDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLH 344
            ++  P EF+PERF+T   KD +++G+NFEL+PFG+GRR CPG + A+Q+ H  L  FLH
Sbjct: 418 KVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLH 477

[Close]

BLAST of HG10014075 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.6e-58
Identity = 150/391 (38.36%), Postives = 216/391 (55.24%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDM---SLNGAGRVSVEMRTWFGNVAL 60
           +RKI ++E+LS+ RV+ L+ V +S+V  S+ EL+D+     N +   SVE++ WF     
Sbjct: 137 LRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPIF 196

Query: 61  NTVLKVIMGKR-----CVGPNGEGC----EEVRRKS--FRRRLRIRFVLWME----AKKV 120
           N VL++++GKR           E C    +E  R +  F     I ++ W++     K +
Sbjct: 197 NMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKAM 256

Query: 121 KSGRKALYYWASSGCSLVFLV-ALGIFLLDTKENGDFMDSLLFLLDRNKLPTCFDGDTII 180
           K   K L    S          ALG  +   +   DFM+ +L  LD  K     D DT+I
Sbjct: 257 KETAKELDVMISEWLEEHRQKRALGEGVDGAQ---DFMNVMLSSLD-GKTIDGIDADTLI 316

Query: 181 KATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVY 240
           K+T+L++I  GTE++  T+ WA+ L+L NP  +E  + ELD  VG++R + ESDIS LVY
Sbjct: 317 KSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICESDISNLVY 376

Query: 241 IQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPFEF 300
           +QA++KET+RLY   PL                             I  D  +W +PFEF
Sbjct: 377 LQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEF 436

Query: 301 KPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPE 344
           KP+RFLTTHKD+++KG +F+LLPFG+GRR CPG++F LQ  H  L  FLH F + N + E
Sbjct: 437 KPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTE 496

[Close]

BLAST of HG10014075 vs. ExPASy TrEMBL
Match: A0A6J1DJE5 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 3.4e-96
Identity = 203/388 (52.32%), Postives = 255/388 (65.72%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGRVSVEMRTWFGNVALNTV 60
           MRKI+  ELLSN RVD +K +M +Q+DMSL ELH     G+ ++ VEM+ WFG+V +N +
Sbjct: 182 MRKITHLELLSNSRVDQVKNLMFAQIDMSLAELH----AGSAQIPVEMKRWFGDVIVNML 241

Query: 61  LKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVK----SGRKAL--YYWAS 120
           LK+I+GKRCVGPN EG E+   K  +  +R  F L  +          GR          
Sbjct: 242 LKIIIGKRCVGPNAEGGEK-GAKDLQMAIRDSFHLMGQGLLTDYIPLIGRLGFDGQVKVM 301

Query: 121 SGCSLVFLVALGIFL----------LDTKENGDFMDSLLFLLDRNKLPTCFDGDTIIKAT 180
              +  F V +  +L          L   ++GDFMDSL+ LLD   +   +D DTIIKAT
Sbjct: 302 ESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLISLLDGKGIQGYYDADTIIKAT 361

Query: 181 MLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVYIQA 240
            L+MI+GGTESTTVTLTW ++L+LNNPHT+E AQQELD +VGR+RRVNESDISKL Y+QA
Sbjct: 362 TLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAVVGRDRRVNESDISKLHYLQA 421

Query: 241 IIKETMRLYPATPL-----------------------------IQIDHVIWPNPFEFKPE 300
           ++KE MR+YPA PL                             IQ D  +WPNPFEFKPE
Sbjct: 422 VVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPNIWKIQTDPRVWPNPFEFKPE 481

Query: 301 RFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPELID 344
           RFLTTHKDV+ KG +FEL+PFG+GRRGCPGVAF LQ+ HFTL GFL  F VK+S+ +LID
Sbjct: 482 RFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSFDVKSSSADLID 541

[Close]

BLAST of HG10014075 vs. ExPASy TrEMBL
Match: A0A1S3CMR1 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 1.3e-95
Identity = 201/393 (51.15%), Postives = 254/393 (64.63%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           +RK++  ELLSN RVD  K  M  +V  SL ELH+      +G+G+V VEM+ WFG+V +
Sbjct: 140 IRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQKDGSGQVEVEMKRWFGDVIV 199

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYW----- 120
           N +LK+I+GKRCVGPN +G E+ + K F+  +R  F L    + +      L  W     
Sbjct: 200 NMLLKIIIGKRCVGPNAKGGEK-QAKDFQLAIRDSFHLM--GQGLLRDYVPLIGWLGFNG 259

Query: 121 ---ASSGCSLVFLVALGIFLLDTKEN----------GDFMDSLLFLLDRNKLPTCFDGDT 180
                   +  F V L  +L + K N          GDFMD+L+ L D   +   +DGDT
Sbjct: 260 QVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALVSLYDGKGIEGYYDGDT 319

Query: 181 IIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKL 240
           IIKAT L+M++GGTESTTVTLTWA+SL+LNNPH +ERAQQELD +VGR+RR+ ESDI  L
Sbjct: 320 IIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDTVVGRDRRLKESDIPNL 379

Query: 241 VYIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPF 300
           VY+Q+IIKETMR+YPA PL                             IQ D  +WP+PF
Sbjct: 380 VYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNLWRIQTDPRVWPDPF 439

Query: 301 EFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNST 344
           +FKPERFLTTHK+V+LKG NFEL+PFG+GRRGCPG AF LQ+ HF L GFLH F V+N T
Sbjct: 440 KFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHFALAGFLHSFDVRNPT 499

[Close]

BLAST of HG10014075 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.4e-94
Identity = 196/391 (50.13%), Postives = 258/391 (65.98%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           +RK++  ELLSN RVD  K  M  +V  SL ELH+      +G+G+V VEM+ WFG+V +
Sbjct: 140 IRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKDGSGQVEVEMKRWFGDVIV 199

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLW----------MEAKKVKSGRK 120
           N +LK+I+GKRCVGPN EG E+ + K F+  +R  F L           +  +   +G+ 
Sbjct: 200 NMLLKIIIGKRCVGPNAEGGEK-QAKDFQLAIRDSFHLMGQGLLRDYIPLIGRLGFNGQV 259

Query: 121 ALYYWASSGCSLVFLVALGIFLLDT------KENGDFMDSLLFLLDRNKLPTCFDGDTII 180
            +    ++   +V    L    L+       +++GDFMD+L+ L D  ++   +DGDTII
Sbjct: 260 KVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVSLYDGKEIEGYYDGDTII 319

Query: 181 KATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVY 240
           KAT L+M++GGTESTTVTLTWA+SL++NNPH +ERAQQELD +VGR+R++ ESDI  LVY
Sbjct: 320 KATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTVVGRDRQLKESDIPNLVY 379

Query: 241 IQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPFEF 300
           +++IIKETMR+YPA PL                             IQ D  +WP+PFEF
Sbjct: 380 LKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNIWKIQTDPRVWPDPFEF 439

Query: 301 KPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPE 344
           KPERFLTTHK+V+LKG NFEL+PFG+GRRGCPG+AF LQ+ HF L GFLH F VKN T E
Sbjct: 440 KPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHFALAGFLHSFDVKNPTKE 499

[Close]

BLAST of HG10014075 vs. ExPASy TrEMBL
Match: A0A6J1FBC6 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 3.5e-93
Identity = 202/397 (50.88%), Postives = 256/397 (64.48%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHD---MSLNGAGRVSVEMRTWFGNVAL 60
           MRKIS   LLSN RVD  K +MLS++  S+ ELH       N  G + VEM+ WFG+V L
Sbjct: 136 MRKISHLHLLSNGRVDQAKSMMLSEMATSVTELHTRWASEKNQLGHIKVEMKRWFGDVTL 195

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSGRKALYYWASSG- 120
           N +LKVI+GKRCVGPN +G +E      +  +R  F L  E       R  +   A+ G 
Sbjct: 196 NMLLKVIIGKRCVGPNAKG-DEKEAIDLQMAIRKSFHLMGEGML----RDYIPILANLGF 255

Query: 121 ---CSLVFLVALGI------------------FLLDTKENGDFMDSLLFLLDRNKLPTCF 180
                 +  +A+ I                    LDTK+ GDFMDSLL L   N+LP+ F
Sbjct: 256 DRHVKAMEKIAIRIDSILQGWLQEHRRNKAKNQSLDTKD-GDFMDSLLSLCRSNELPSQF 315

Query: 181 DGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESD 240
           D DTI+KAT+L+MI+GGTES++VTLTWA+SL++NNP  +E+A +ELD++VGR+RRVNESD
Sbjct: 316 DHDTIVKATILNMIAGGTESSSVTLTWALSLLVNNPGALEKAYEELDQVVGRDRRVNESD 375

Query: 241 ISKLVYIQAIIKETMRLYPATPL-----------------------------IQIDHVIW 300
           I+KLVY+QA++KETMRLYPA PL                             IQ D  +W
Sbjct: 376 ITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIPNIWKIQTDPRVW 435

Query: 301 PNPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHV 344
           P+PFEFKPERFLTTH DV+LKG NFEL+PFG+GRRGCP VAF LQ+ HF L G LH F V
Sbjct: 436 PDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMVHFALAGLLHSFTV 495

[Close]

BLAST of HG10014075 vs. ExPASy TrEMBL
Match: A0A6J1FCU2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 4.6e-93
Identity = 198/399 (49.62%), Postives = 258/399 (64.66%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELH---DMSLNGAGRVSVEMRTWFGNVAL 60
           MRKIS   LLSN+RVD +K +MLS++  S+ ELH       N  G ++VEM+ WFG+V  
Sbjct: 136 MRKISHLHLLSNRRVDQVKSMMLSEMATSVTELHARWASEKNQMGHINVEMKRWFGDVIA 195

Query: 61  NTVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVLWMEAKKVKSG--RKALYYWASS 120
           N +LKVI+GKRCVGPN +G E       +  + ++  +      +  G  R  +   A+ 
Sbjct: 196 NMLLKVIIGKRCVGPNAKGDE-------KEAIDLQMAIGKSFHLMGEGMLRDYIPILANL 255

Query: 121 G-----------CSLVFLVALGIF-----------LLDTKENGDFMDSLLFLLDRNKLPT 180
           G            + +  +  G              LDTK+ GDFMDSLL L   N+LP 
Sbjct: 256 GFDRHSKAMEKIATRIDSILQGWLQEHRRNRDKNQSLDTKD-GDFMDSLLSLCRTNELPR 315

Query: 181 CFDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNE 240
            FD DTI+KAT L+MI+GGTE++TVTLTWA+SL++NNP  +E+A +ELD++VGR+RRVNE
Sbjct: 316 HFDHDTIVKATTLNMIAGGTETSTVTLTWALSLLVNNPGALEKAYEELDQVVGRDRRVNE 375

Query: 241 SDISKLVYIQAIIKETMRLYPATPL-----------------------------IQIDHV 300
           SDI+KLVY+QA++KETMRLYPA PL                             IQ D  
Sbjct: 376 SDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIPNIWKIQTDPR 435

Query: 301 IWPNPFEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLF 344
           +WP+PFEFKPERFLTTH DV+LKG NFEL+PFG+GRRGCPG+AF LQ+ HF L GFLH F
Sbjct: 436 VWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFALAGFLHSF 495

[Close]

BLAST of HG10014075 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 231.9 bits (590), Expect = 7.9e-61
Identity = 148/394 (37.56%), Postives = 220/394 (55.84%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGR--VSVEMRTWFGNVALN 60
           MRKI+  ELLSN+R+ MLK V +S++ M + +L+ +     G   V V++++W  ++ LN
Sbjct: 130 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLN 189

Query: 61  TVLKVIMGKRCVGPNGEGCEEVRRKSFRRRLRI-RFVLWMEAKKVKSGRKALYYW----- 120
            +++++ GKR  G  G    E   ++ + +  I +F   +    V      L ++     
Sbjct: 190 MIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGH 249

Query: 121 ----ASSGCSLVFLVALGI-------FLLDTKEN-GDFMDSLLFLLDRNKLPTC-FDGDT 180
                 +G  L  ++   I           TKEN  DF+D ++ L ++ KL    +D +T
Sbjct: 250 EKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQYDANT 309

Query: 181 IIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKL 240
            IK+T L++I GG++++  TLTWAISL+LNN   +++AQ E+D  VGR+R V +SDI  L
Sbjct: 310 SIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENL 369

Query: 241 VYIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPF 300
           VY+QAIIKET+RLYPA PL                             IQ D  ++  P 
Sbjct: 370 VYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPN 429

Query: 301 EFKPERFLTTH-KDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNS 344
           EF+PERF+T   K+ +++G+NFEL+PFG+GRR CPG + A+QV H  L  FLH F VK  
Sbjct: 430 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFDVKTV 489

[Close]

BLAST of HG10014075 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 229.6 bits (584), Expect = 3.9e-60
Identity = 150/401 (37.41%), Postives = 220/401 (54.86%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDM--SLNGAGRVSVEMRTWFGNVALN 60
           MRKI++ ELLSN+R+ ML  V +S++ M + +L+ +     G+  V V++++W  ++  N
Sbjct: 118 MRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIAN 177

Query: 61  TVLKVIMGKRCVGPNG----EGCEEVR--RKSFRRRLRIRFVL----------WME---- 120
            +++++ GKR  G  G    E  EE R  RK   +   +  +           W++    
Sbjct: 178 MIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGH 237

Query: 121 AKKVKSGRK----ALYYWASSGCSLVFLVALGIFLLDTKEN-GDFMDSLLFLLDRNKLPT 180
            K++K  R+     L  W  +      +         TK N  DF+D +L L ++ KL  
Sbjct: 238 EKEMKQTRRELDVILERWIENHRQQRKVSG-------TKHNDSDFVDVMLSLAEQGKLSH 297

Query: 181 C-FDGDTIIKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVN 240
             +D +T IK T L++I GG+E++  TLTWAISL+LNN   +++ Q E+D  VGR+R V 
Sbjct: 298 LQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVE 357

Query: 241 ESDISKLVYIQAIIKETMRLYPATPL-----------------------------IQIDH 300
           +SDI  LVY+QAIIKET+RLYPA PL                             IQ D 
Sbjct: 358 DSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDP 417

Query: 301 VIWPNPFEFKPERFLTTH-KDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLH 344
            ++  P EF+PERF+T   KD +++G+NFEL+PFG+GRR CPG + A+Q+ H  L  FLH
Sbjct: 418 KVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLH 477

[Close]

BLAST of HG10014075 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 219.9 bits (559), Expect = 3.1e-57
Identity = 150/393 (38.17%), Postives = 216/393 (54.96%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDM--SLNGAGRVSVEMRTWFGNVALN 60
           MRKI+  ELLSN+R+ MLK V +S++ M + +L+ +     G+  V V++++W  +++LN
Sbjct: 130 MRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLN 189

Query: 61  TVLKVIMGKRCVGPNG---EGCEEVR--RKSFRRRLRIRFVLWMEAKKVKSG----RKAL 120
            +++++ GKR  G      E  EE R  RK       +  +  +     K G    +   
Sbjct: 190 MMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHE 249

Query: 121 YYWASSGCSLVFLVALGI-------FLLDTKEN-GDFMDSLLFLLDRNKLPTC-FDGDTI 180
                +G  L  ++   I        +  TK N  DF+D +L L ++ K      D  T 
Sbjct: 250 KEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAITS 309

Query: 181 IKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLV 240
           IK+T L++I GG+E++  TLTWAISL+LNN   +++AQ E+D  VGR+R V +SDI  LV
Sbjct: 310 IKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLV 369

Query: 241 YIQAIIKETMRLYPATPL-----------------------------IQIDHVIWPNPFE 300
           YIQAIIKET+RLYPA PL                             IQ D  ++  P E
Sbjct: 370 YIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNE 429

Query: 301 FKPERFLTTH-KDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNST 344
           F+PERF+T   K+ +++G+NFEL+PFG+GRR CPG + A+QV H  L  FL  F VK   
Sbjct: 430 FRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVM 489

[Close]

BLAST of HG10014075 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 203.0 bits (515), Expect = 3.9e-52
Identity = 134/394 (34.01%), Postives = 202/394 (51.27%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGR----VSVEMRTWFGNVA 60
           +RKI++SEL S   VDM       + D++   L+ +     G+    V V+M+  F ++ 
Sbjct: 130 IRKIAVSELFSTSGVDMHMVSRAREADLAFRALY-VRWEKRGKPKEGVLVDMKQEFIDLT 189

Query: 61  LNTVLKVIMGKRCVG--PNGEGCEEVRRKSFRRRLRIRFVLWMEAK----------KVKS 120
            N  L ++ GKR  G  PN E  E  R     R     F L++ +           K K 
Sbjct: 190 ANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLDWKTKR 249

Query: 121 GRKALYYWASSGCSLVFLVALGIFLLDTKENG----DFMDSLLFLLDRNKLPTCFDGDTI 180
           G K      + G   V    +        ++G    D++D L+ +L ++K+P   D  T 
Sbjct: 250 GMKR----TAKGLDKVAEGWIEEHKNKRSDHGRSENDYLDILIKILGQDKIPGLSDTHTK 309

Query: 181 IKATMLSMISGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLV 240
           IKA  L+++  G+E+  V L WA+SL+LNNPH + +AQ+ELD  +G+ER V E DI  LV
Sbjct: 310 IKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLV 369

Query: 241 YIQAIIKETMRLYPATPL-------------------------------IQIDHVIWPNP 300
           Y+QAI+KET RLYP  PL                               I  D  +W NP
Sbjct: 370 YLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNP 429

Query: 301 FEFKPERFLTTHKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNS 344
            +F+PERFLT+++++++ G++++  PFG GRR CP +   +++ H+ LV FLH F +   
Sbjct: 430 EQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARP 489

[Close]

BLAST of HG10014075 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 182.6 bits (462), Expect = 5.5e-46
Identity = 127/384 (33.07%), Postives = 187/384 (48.70%), Query Frame = 0


Query: 1   MRKISISELLSNQRVDMLKPVMLSQVDMSLNELHDMSLNGAGRVSVEMRTWFGNVALNTV 60
           +RKI    L SN  ++ML  +  S+V+  +  L+       G   V++   F  +  N +
Sbjct: 136 LRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK---GNGGTSIVKIDMLFEFLTFNII 195

Query: 61  LKVIMGKRCVGPNGEGCEEVRRKSFRRRLRIRFVL-----------WMEAKKVKSGRKAL 120
           L+ ++GKR +G      +E R K   +      V+           W++  K    ++  
Sbjct: 196 LRKMVGKR-IGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKNSQMKRLF 255

Query: 121 YYWASSGCSLVFLVALGIFLLDTKENGDFMDSLLFLLDRNKLPTCFDGDTIIKATMLSMI 180
               S     +          +  +    MD LL +L  + + +    D I+KAT+L++ 
Sbjct: 256 KELDSVNTKWLHEHLKKRSRNEKDQERTIMDLLLDILPEDIVISGHVRDVIVKATILALT 315

Query: 181 SGGTESTTVTLTWAISLMLNNPHTIERAQQELDEIVGRERRVNESDISKLVYIQAIIKET 240
             G++ST++TLTWA+SL+LNNP  +E AQ+E+D  VG+ R + ESDI  L Y+QAI+KET
Sbjct: 316 LTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKET 375

Query: 241 MRLYPATPLIQI-----------------------------DHVIWPNPFEFKPERFLTT 300
            RLYP  PL  I                             D  IWP+P  FKPERF+  
Sbjct: 376 HRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMED 435

Query: 301 HKDVELKGKNFELLPFGTGRRGCPGVAFALQVEHFTLVGFLHLFHVKNSTPELIDMSERY 345
               E    NFE +PFG+GRR CPGV   L+V HF L   L  F +   + E +DM+E  
Sbjct: 436 KSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGP 495

The following BLAST results are available for this feature:

BLAST of HG10014075 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897329.1	2.0e-127	65.26	cytochrome P450 82A3-like [Benincasa hispida]	[more]
XP_022154118.1	7.1e-96	52.32	cytochrome P450 82A3-like [Momordica charantia]	[more]
XP_008464762.1	2.7e-95	51.15	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo]	[more]
XP_004147824.1	3.0e-94	50.13	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_...	[more]
XP_038897047.1	1.5e-93	51.15	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]

BLAST of HG10014075 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43068	1.0e-65	37.47	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
O49858	1.8e-62	38.07	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
Q9SZ46	1.1e-59	37.56	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O49396	5.5e-59	37.41	Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3	[more]
O49859	3.6e-58	38.36	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]

BLAST of HG10014075 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJE5	3.4e-96	52.32	cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV...	[more]
A0A1S3CMR1	1.3e-95	51.15	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1	[more]
A0A0A0LD43	1.4e-94	50.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1	[more]
A0A6J1FBC6	3.5e-93	50.88	cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=...	[more]
A0A6J1FCU2	4.6e-93	49.62	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3...	[more]

BLAST of HG10014075 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	7.9e-61	37.56	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31950.1	3.9e-60	37.41	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT4G31970.1	3.1e-57	38.17	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT2G25160.1	3.9e-52	34.01	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	5.5e-46	33.07	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 194..214
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 133..238
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 239..343 coord: 1..80
None	No IPR available	PANTHER	PTHR47947:SF18	CYTOCHROME P450 82A2	coord: 239..343 coord: 1..80
None	No IPR available	PANTHER	PTHR47947:SF18	CYTOCHROME P450 82A2	coord: 133..238
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 283..306 score: 23.59 coord: 160..177 score: 23.19 coord: 180..206 score: 33.81 coord: 235..259 score: 34.61 coord: 273..283 score: 55.26
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 224..235 score: 44.86 coord: 274..283 score: 58.96 coord: 171..188 score: 31.35
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 134..244 e-value: 7.3E-24 score: 84.3 coord: 242..317 e-value: 3.0E-15 score: 56.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..333 e-value: 4.5E-56 score: 192.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..337
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 276..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014075.1	HG10014075.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen