HG10014065 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014065 vs. NCBI nr
Match: XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.3 bits (1749), Expect = 4.6e-191
Identity = 353/527 (66.98%), Postives = 397/527 (75.33%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME    +AGII   + F Y      RRSV HRK++PPEA GAWPVIGHLHLL+A EPTHI
Sbjct: 1   MEHLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGEPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
           TLAKMADAYGPIFT RFGMKKALIVS+WEIAKEFFTTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  TLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           +GFSPYTPHWR  RKIATLELLTNHRLEQ QHIRAFEV++WM EL+++ +     K+N +
Sbjct: 121 IGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSI-----KSNER 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+AVEMKK LAD+T+N IFKMVIGKRFS ++ G ++ F KAL++FFGLF IF+P+DSF
Sbjct: 181 GEKVAVEMKKWLADITLNTIFKMVIGKRFSAIQHG-NENFPKALIDFFGLFHIFVPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT K+ID  F KFL++HR+R N+   E E  E DFMDVMIS V 
Sbjct: 241 PFLSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNDA--EAETAEQDFMDVMISRV- 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           ++DGQHFSYD DTVIKAT L                              AQLELD +VG
Sbjct: 301 EEDGQHFSYDADTVIKATSLNMVLAGFDTTTITMVWALSLLLNNEGVLENAQLELDEKVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           R R VKESDVKNL YL+                           AGYHI KGTRLIVN+Q
Sbjct: 361 RSRQVKESDVKNLPYLRAIVKETLRLYPAAPLLVPHESIEDCTAAGYHISKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 467
           KLQKDPLVWE PC+FRPERFLTSQK+FDVRGQSPQ IPFGNGRRMCP ISFAL+++HLTL
Sbjct: 421 KLQKDPLVWEGPCEFRPERFLTSQKDFDVRGQSPQFIPFGNGRRMCPAISFALQMIHLTL 480

BLAST of HG10014065 vs. NCBI nr
Match: XP_011652397.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_002536 [Cucumis sativus])

HSP 1 Score: 677.9 bits (1748), Expect = 6.0e-191
Identity = 351/526 (66.73%), Postives = 403/526 (76.62%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME  + + GII  L+ FFY +    RRSV  RK+LPP+A GAWPVIGHLHLLNA+EPTHI
Sbjct: 1   MEYLNTLFGIIFPLLIFFYLLFTCSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
           TLAKMADAYGP+FT RFGMK+ALIVSNW++AKE FTTNDRIFASRPKLVASK+L YDYAM
Sbjct: 61  TLAKMADAYGPMFTFRFGMKRALIVSNWDLAKEIFTTNDRIFASRPKLVASKILAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPY+PHWR+ RKIATLELLTNHR++Q Q+IRAFEV+ WM+ELYEL       + NNK
Sbjct: 121 MGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMEELYELW------RLNNK 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+ VEMKK+LADVT+N +FKMVIGK+FS+ME G ++KFQK L+ FFGLF IF+ +DSF
Sbjct: 181 GEKVVVEMKKRLADVTLNTMFKMVIGKKFSSMEYG-NEKFQKVLIEFFGLFGIFILSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGH++VMKKT KI+D VFDKFLKEHR+R NN   E+   E DFMDVMIS V 
Sbjct: 241 PFLSWLDLGGHKKVMKKTAKIMDEVFDKFLKEHRERINNF-GELPAAEKDFMDVMISTV- 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           +DDGQHF+   DTVIKATCL                              AQ+ELD QVG
Sbjct: 301 EDDGQHFNCHVDTVIKATCLNMILGGFDTTTVTMTWALCLLLNNKEALKKAQVELDEQVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           RER VKE+D+KNL YLQ                           AGYHIPKGTRLIVN+Q
Sbjct: 361 RERQVKETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 466
           KLQ+DPLVWEDP +FRPERFLTSQKNFDVRGQ+PQ IPFGNGRRMCP ISFAL+I++LTL
Sbjct: 421 KLQRDPLVWEDPFEFRPERFLTSQKNFDVRGQNPQFIPFGNGRRMCPAISFALQIIYLTL 480

BLAST of HG10014065 vs. NCBI nr
Match: XP_023535451.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.2 bits (1746), Expect = 1.0e-190
Identity = 347/527 (65.84%), Postives = 398/527 (75.52%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFY----SIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME  +A+AGII   +FF Y    S RRSV HRK++PPEA GAWP+IGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFLYVLFTSSRRSVAHRKRIPPEAGGAWPLIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
            LAKMADAYGP+FTLRFG+KKAL+VS+WEIAKEF TTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  ALAKMADAYGPMFTLRFGLKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPYTPHWR  RKIATLELLTNHRLEQ QHIRAFEV++WM EL+++ +     K+N +
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMNELHKMSI-----KSNER 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+AVEMKK LAD+T+N IFKMVIGKRFS ++ G ++ F KAL++FFGLF IF+P+DSF
Sbjct: 181 GEKVAVEMKKWLADITLNTIFKMVIGKRFSAIQHG-NENFPKALIDFFGLFHIFVPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT K+ID  F KFL++HR+R N+   E E  E DFMDVMI+ V 
Sbjct: 241 PFLSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRNDA--EAETAEKDFMDVMIARV- 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           ++DGQHFSYD DTVIKATCL                               QLELD  VG
Sbjct: 301 EEDGQHFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALNKVQLELDENVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           R R V+ESD+KNL YL+                           AGYHI KGTRLIVN+Q
Sbjct: 361 RSRQVEESDIKNLPYLRAIVKETLRLYPAVPLMAPHESSEDCIVAGYHIYKGTRLIVNLQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 467
           KLQKDP VWE+P +FRPERFLTSQKNFDVRG SPQ IPFGNGRRMCP ISFAL++MH+TL
Sbjct: 421 KLQKDPRVWEEPSEFRPERFLTSQKNFDVRGVSPQFIPFGNGRRMCPAISFALQVMHITL 480

BLAST of HG10014065 vs. NCBI nr
Match: XP_008464769.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 676.0 bits (1743), Expect = 2.3e-190
Identity = 351/526 (66.73%), Postives = 397/526 (75.48%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           M Q + + GII  L+  FY +    RRSV  RK+LPP+A GAWPVIGHLHLLNA+EPTHI
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
           T AKMADAYGP+FT RFGM +ALIVSNWE+AKEFFTTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPY+PHWRH RKIATLELLTNHR+ Q Q+IRAFE+K WMKELYEL       + NNK
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELW------RLNNK 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+ VEMKK+LAD+T+N IFKMVIGKRFS++E G ++KFQ  L+ FFGLF IF+P+DSF
Sbjct: 181 GEKVVVEMKKRLADITLNTIFKMVIGKRFSSIEYG-NEKFQNVLIEFFGLFGIFIPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT KIID VFDKFLKE R+R NN   EVE  E  FMD+MIS V+
Sbjct: 241 PFLSWLDLGGHEKAMKKTAKIIDEVFDKFLKERRERINNF-GEVEAAEKGFMDIMISTVQ 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
            DDGQHF+   DTVIKATCL                              AQ+EL+ QVG
Sbjct: 301 -DDGQHFNCHVDTVIKATCLNMILGGFDTTTITMTWALCLLLNNKEELKKAQVELNEQVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           RER V+E+D+KNL YLQ                           AGYHIPKGTRLIVN+Q
Sbjct: 361 RERQVEETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 466
           KLQKDP+VW+DP +FRPERFLTSQKNFDVRGQ PQ IPFGNGRRMCP ISFAL+I++LTL
Sbjct: 421 KLQKDPVVWQDPFEFRPERFLTSQKNFDVRGQHPQFIPFGNGRRMCPAISFALQIIYLTL 480

BLAST of HG10014065 vs. NCBI nr
Match: XP_022935746.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 3.0e-190
Identity = 349/527 (66.22%), Postives = 397/527 (75.33%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFY----SIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME  +A+AGII   +FF Y    S RRSV HRK++PPEA GAWP+IGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFLYVLFTSSRRSVAHRKRIPPEAGGAWPLIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
            LAKMADAYGP+FTLRFGMKKAL+VS+WEIAKEF TTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  VLAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPYTPHWR  RKIATLELLTNHRLEQ QHIRAFEV++WMKEL+++ +     K+N +
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSI-----KSNER 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+AVEMKK LAD+T+N IFKMVIGKRFS ++ G+ + F KAL++FFGLF IF+P+DSF
Sbjct: 181 GEKVAVEMKKWLADITLNTIFKMVIGKRFSAIQHGT-ENFPKALIDFFGLFHIFVPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT KIID  F KFL++HR+R N+   E E  E DFMDVMI+ V 
Sbjct: 241 PFLSWLDLGGHEKTMKKTAKIIDEAFHKFLQQHRERRND--GEAETAEKDFMDVMIARV- 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           ++DGQHFSYD DT+IKATCL                               QLELD +VG
Sbjct: 301 EEDGQHFSYDADTIIKATCLNMIVGGFDTTAVTMTWALCLLLNNEETLKKVQLELDEKVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           R R VKESDVKNL YLQ                           AGYHI KGTRLIVN+Q
Sbjct: 361 RSRQVKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 467
           KLQKDP VWE+P +FRPERFLT QKNFDVRG SPQ IPFGNGRR CP ISFAL++MH+TL
Sbjct: 421 KLQKDPRVWEEPSEFRPERFLTGQKNFDVRGLSPQFIPFGNGRRRCPAISFALQVMHITL 480

BLAST of HG10014065 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.3e-108
Identity = 213/517 (41.20%), Postives = 302/517 (58.41%), Query Frame = 0

Query: 11  ILALVFFFYSIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMADAYGPIFT 70
           IL+L+F    + R    R K  P  SGAWP++GHL LLN ++  H  L  +AD YGP+FT
Sbjct: 18  ILSLIFLCLFLYRK-NSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFT 77

Query: 71  LRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHAR 130
           ++ GMK AL++SNWE++KE FTTND   +SRPKLVA +++ Y+ A +G +PY P+WR  R
Sbjct: 78  IKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWRELR 137

Query: 131 KIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLAD 190
           KI T E L+N R+EQ  HIR  EV+  +KEL+++      + N N+     V++K+ LA 
Sbjct: 138 KIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIW----SNGNKNESRYTLVDIKQWLAY 197

Query: 191 VTMNMIFKMVIGKRFSNM--EDGSD--QKFQKALMNFFGLFSIFLPADSFPFLSWLDLGG 250
           +T NM+ +MV+GKR+  +   +G D  Q+F K +  F  L   F  AD  P L WLDLGG
Sbjct: 198 LTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGG 257

Query: 251 HERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQHFSYD 310
           HE+ MK   K +D +  ++L+EHR +      E    + DFMDVMISA+  +  Q  ++D
Sbjct: 258 HEKAMKANAKEVDKLLSEWLEEHRQK--KLLGENVESDRDFMDVMISAL--NGAQIGAFD 317

Query: 311 DDTVIKATCL------------------------------AQLELDNQVGRERHVKESDV 370
            DT+ KAT L                              A+ E+D Q+G++ +++ESD+
Sbjct: 318 ADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDI 377

Query: 371 KNLSYLQA---------------------------GYHIPKGTRLIVNIQKLQKDPLVWE 430
             L YLQA                           GYHI KGTRLI N+ K+ +DP VW 
Sbjct: 378 SKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWS 437

Query: 431 DPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLHGFIIS 467
           DP +F+PERFLT+ K+ D+RG + +L+PFG+GRR+C G+S  L ++H TLAN LH F I 
Sbjct: 438 DPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDIL 497

BLAST of HG10014065 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.4e-102
Identity = 208/521 (39.92%), Postives = 309/521 (59.31%), Query Frame = 0

Query: 14  LVFFFYSI-RRSVGHRKKLPPEASGAWPVIGHLHLLNANEP-THITLAKMADAYGPIFTL 73
           LVF F ++ ++S   +    P  SGAWP+IGHLHLL   E   + TL KMAD YGP  +L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 74  RFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHARK 133
           + G  +A +VS++E+AK+ FT ND+  ASRP   A+K +GY++A+ GF+PY+  WR  RK
Sbjct: 73  QLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRK 132

Query: 134 IATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLADV 193
           IAT+ELL+N RL+  +H+R  E+   +K+LY L  KN  +K       + V++K  L D+
Sbjct: 133 IATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK------PVMVDLKSWLEDM 192

Query: 194 TMNMIFKMVIGKRF-------SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWLDL 253
           T+NMI +MV GKR+       S+ +     + +KA+  FF L  IF  +D+FP LS+ DL
Sbjct: 193 TLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDL 252

Query: 254 GGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEH--DFMDVMISAVKDDDGQH 313
            GHE+ MK+T   +D + +++++ HR +    R+    KE+  DF+DVM+S  +     H
Sbjct: 253 QGHEKEMKQTGSELDVILERWIENHRQQ----RKFSGTKENDSDFIDVMMSLAEQGKLSH 312

Query: 314 FSYDDDTVIKATCL------------------------------AQLELDNQVGRERHVK 373
             YD +T IK+TCL                              AQ E+D  VGR+R+V+
Sbjct: 313 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 372

Query: 374 ESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKDP 433
           +SD++NL YLQ                           AGY++P GTRLIVN+ K+Q+DP
Sbjct: 373 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 432

Query: 434 LVWEDPCKFRPERFLTSQ-KNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLH 466
            V+ +P +FRPERF+T + K FDVRGQ+ +L+PFG+GRR CPG S A++++HL LA FLH
Sbjct: 433 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 492

BLAST of HG10014065 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 208/520 (40.00%), Postives = 300/520 (57.69%), Query Frame = 0

Query: 9   GIILALVFFFYSIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMADAYGPI 68
           G  + L  FFY + +   ++ K PP+ +GAWP+IGHLHLL   +  H  L+  AD  GP+
Sbjct: 13  GFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNGPV 72

Query: 69  FTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRH 128
           F ++ G+ +AL+V+N EIAKE FTTNDR F +RP  VA+K++GY+Y M+G +PY P+WR 
Sbjct: 73  FKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRD 132

Query: 129 ARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKL 188
            RKI  LE L+N RL+  +H+   E+    KELY+L       +  N D  + V+MK+ L
Sbjct: 133 MRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLW------ETQNIDFCL-VDMKQWL 192

Query: 189 ADVTMNMIFKMVIGKRF------SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWL 248
           AD+T+NM  KMV+GKRF      S  E+       K L N F L   F+ +D  P+L WL
Sbjct: 193 ADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWL 252

Query: 249 DLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQH 308
           DLGGHE+ MK+T+K +D +F  +L EH+ +  +  +E +  + DFMDVM+S +++     
Sbjct: 253 DLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDD--DQDFMDVMLSILEE---SK 312

Query: 309 FSYDDDTVIKATCL------------------------------AQLELDNQVGRERHVK 368
              D DT+ K  CL                              AQ ELD  VGR+R+V 
Sbjct: 313 LGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVD 372

Query: 369 ESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKDP 428
           ESD+  L+Y+                            AGYH+  GTRLIVN  K+Q+DP
Sbjct: 373 ESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDP 432

Query: 429 LVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLHG 466
           LVW  P +++PERFL  +++ D++GQ  +LIPFG+GRR CP IS AL+++ LTLA+ LHG
Sbjct: 433 LVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHG 492

BLAST of HG10014065 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 368.6 bits (945), Expect = 1.0e-100
Identity = 208/533 (39.02%), Postives = 300/533 (56.29%), Query Frame = 0

Query: 11  ILALVFFFYSIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMADAYGPIFT 70
           +++L+FF +   + V H K+ PP  SG+WP++GHL L+   +  H TL  + D YGPIFT
Sbjct: 16  LISLLFFLFRFSK-VSHTKE-PPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYGPIFT 75

Query: 71  LRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHAR 130
           ++ G   AL++SNWE+AKE FT ND + +SRPK VA +L+ Y+ A +G++PY  +WR  R
Sbjct: 76  IKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWRQLR 135

Query: 131 KIATLELLTNHRLEQFQHIRAFEVKKWMKEL-----------YELCLKNSCSKNNNK--- 190
           KI TLE+L+N R+E   HIR  EV+  +KEL           Y L      S  N++   
Sbjct: 136 KIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDEPST 195

Query: 191 DEKMAVEMKKKLADVTMNMIFKMVIGKR------FSNMEDGSDQKFQKALMNFFGLFSIF 250
            + ++VE+KK  A +T+NM+ +MV+GKR        N E+   ++F + + +F  L   F
Sbjct: 196 TDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEA--KRFLENIRDFMRLIGTF 255

Query: 251 LPADSFPFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDV 310
              D  PFL WLDLGGHE+ MKK  K  D + +++L+EHR++      +  + E DFMD 
Sbjct: 256 TVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFMDA 315

Query: 311 MISAVKDDDGQHFSYDDDTVIKATCLAQL------------------------------E 370
           M+  +KD   + F  D DT+IKAT L  +                              E
Sbjct: 316 MLLVLKDKPIEGF--DVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVLEKLKEE 375

Query: 371 LDNQVGRERHVKESDVKNLSYLQA---------------------------GYHIPKGTR 430
           L+  +G+ER V ESD+  L YL A                           GYHI KGTR
Sbjct: 376 LNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTR 435

Query: 431 LIVNIQKLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALK 467
           L+ N+ K+ +DP VW DP +F+PERFL++ K+ DVRGQ+ +L+PFG+GRRMC G+S  L 
Sbjct: 436 LMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAGMSLGLH 495

BLAST of HG10014065 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.9e-100
Identity = 205/518 (39.58%), Postives = 293/518 (56.56%), Query Frame = 0

Query: 7   VAGIILALVFFFYSIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMADAYG 66
           V  +I  L  F Y   + V    K  P   GAWP+ GHL LL  ++  H  L  +A+ +G
Sbjct: 15  VVSLIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHG 74

Query: 67  PIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHW 126
           P+FT++ G KKAL+VS+WE+A+E FTTND   ++RPKL+ ++L+ Y+ AM+  +PY P+W
Sbjct: 75  PLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYW 134

Query: 127 RHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKK 186
           R  RKI   E+L++ R+EQ Q +R  EV+  + ELY++          N+ +  +VE+K+
Sbjct: 135 RELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDV-----WRSQKNESDYASVELKQ 194

Query: 187 KLADVTMNMIFKMVIGKRF--SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWLDL 246
             A    NM+ +MV+GKRF  +   D   +K  KA+  F  L  +F   D+ P+L WLD 
Sbjct: 195 WFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDF 254

Query: 247 GGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQHF- 306
           GG+E+ MK+T K +D +  ++L+EHR +      E      DFM+VM+S++   DG+   
Sbjct: 255 GGYEKAMKETAKELDVMISEWLEEHRQK--RALGEGVDGAQDFMNVMLSSL---DGKTID 314

Query: 307 SYDDDTVIKATCLA-----------------------------QLELDNQVGRERHVKES 366
             D DT+IK+T L                              + ELD QVG++R + ES
Sbjct: 315 GIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILENKAELDIQVGKDRCICES 374

Query: 367 DVKNLSYLQA---------------------------GYHIPKGTRLIVNIQKLQKDPLV 426
           D+ NL YLQA                           GYH+ KGTRLI NI K+  DP V
Sbjct: 375 DISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNV 434

Query: 427 WEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLHGFI 466
           W DP +F+P+RFLT+ K+ DV+G   QL+PFG+GRR+CPGISF L+ +HL LA+FLH F 
Sbjct: 435 WSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFE 494

BLAST of HG10014065 vs. ExPASy TrEMBL
Match: A0A1S3CMD6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.1e-190
Identity = 351/526 (66.73%), Postives = 397/526 (75.48%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           M Q + + GII  L+  FY +    RRSV  RK+LPP+A GAWPVIGHLHLLNA+EPTHI
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
           T AKMADAYGP+FT RFGM +ALIVSNWE+AKEFFTTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPY+PHWRH RKIATLELLTNHR+ Q Q+IRAFE+K WMKELYEL       + NNK
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELW------RLNNK 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+ VEMKK+LAD+T+N IFKMVIGKRFS++E G ++KFQ  L+ FFGLF IF+P+DSF
Sbjct: 181 GEKVVVEMKKRLADITLNTIFKMVIGKRFSSIEYG-NEKFQNVLIEFFGLFGIFIPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT KIID VFDKFLKE R+R NN   EVE  E  FMD+MIS V+
Sbjct: 241 PFLSWLDLGGHEKAMKKTAKIIDEVFDKFLKERRERINNF-GEVEAAEKGFMDIMISTVQ 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
            DDGQHF+   DTVIKATCL                              AQ+EL+ QVG
Sbjct: 301 -DDGQHFNCHVDTVIKATCLNMILGGFDTTTITMTWALCLLLNNKEELKKAQVELNEQVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           RER V+E+D+KNL YLQ                           AGYHIPKGTRLIVN+Q
Sbjct: 361 RERQVEETDLKNLPYLQAIVKETLRLYPAAPLLVPHESIEDCTVAGYHIPKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 466
           KLQKDP+VW+DP +FRPERFLTSQKNFDVRGQ PQ IPFGNGRRMCP ISFAL+I++LTL
Sbjct: 421 KLQKDPVVWQDPFEFRPERFLTSQKNFDVRGQHPQFIPFGNGRRMCPAISFALQIIYLTL 480

BLAST of HG10014065 vs. ExPASy TrEMBL
Match: A0A6J1F5K6 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.5e-190
Identity = 349/527 (66.22%), Postives = 397/527 (75.33%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFY----SIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME  +A+AGII   +FF Y    S RRSV HRK++PPEA GAWP+IGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFLYVLFTSSRRSVAHRKRIPPEAGGAWPLIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
            LAKMADAYGP+FTLRFGMKKAL+VS+WEIAKEF TTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  VLAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPYTPHWR  RKIATLELLTNHRLEQ QHIRAFEV++WMKEL+++ +     K+N +
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSI-----KSNER 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            EK+AVEMKK LAD+T+N IFKMVIGKRFS ++ G+ + F KAL++FFGLF IF+P+DSF
Sbjct: 181 GEKVAVEMKKWLADITLNTIFKMVIGKRFSAIQHGT-ENFPKALIDFFGLFHIFVPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT KIID  F KFL++HR+R N+   E E  E DFMDVMI+ V 
Sbjct: 241 PFLSWLDLGGHEKTMKKTAKIIDEAFHKFLQQHRERRND--GEAETAEKDFMDVMIARV- 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           ++DGQHFSYD DT+IKATCL                               QLELD +VG
Sbjct: 301 EEDGQHFSYDADTIIKATCLNMIVGGFDTTAVTMTWALCLLLNNEETLKKVQLELDEKVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           R R VKESDVKNL YLQ                           AGYHI KGTRLIVN+Q
Sbjct: 361 RSRQVKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 467
           KLQKDP VWE+P +FRPERFLT QKNFDVRG SPQ IPFGNGRR CP ISFAL++MH+TL
Sbjct: 421 KLQKDPRVWEEPSEFRPERFLTGQKNFDVRGLSPQFIPFGNGRRRCPAISFALQVMHITL 480

BLAST of HG10014065 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.6e-189
Identity = 346/527 (65.65%), Postives = 396/527 (75.14%), Query Frame = 0

Query: 1   MEQSHAVAGIILALVFFFY----SIRRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHI 60
           ME  +A+AGII   +FF Y    S RRSV HRK++PPEA GAWPVIGHLHLL+A +PTHI
Sbjct: 1   MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAM 120
            LAKMADAYGP+FTLRFGMKKAL+VS+WEIAKEF TTNDRIFASRPKLVASKLL YDYAM
Sbjct: 61  ALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MGFSPYTPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNK 180
           MGFSPYTPHWR  RKIATLELLTNHRLEQ QHIR FEV++WMKEL+++ +     ++N +
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSI-----RSNER 180

Query: 181 DEKMAVEMKKKLADVTMNMIFKMVIGKRFSNMEDGSDQKFQKALMNFFGLFSIFLPADSF 240
            +K+AVEMKK LAD+T+N IFKMVIGKRFS ++ G+ + F KAL++FFGLF IF+P+DSF
Sbjct: 181 GDKVAVEMKKWLADITLNTIFKMVIGKRFSAIQHGT-ENFPKALIDFFGLFHIFVPSDSF 240

Query: 241 PFLSWLDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVK 300
           PFLSWLDLGGHE+ MKKT K+ID  F KFL++HR+R N    + E  E DFMDVMIS V+
Sbjct: 241 PFLSWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERRN--EGQAETAEQDFMDVMISRVE 300

Query: 301 DDDGQHFSYDDDTVIKATCL------------------------------AQLELDNQVG 360
           +D+ QHFSYD DTVIKATCL                               QLELD +VG
Sbjct: 301 EDE-QHFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVG 360

Query: 361 RERHVKESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQ 420
           R   VKESDVKNL YLQ                           AGYHI KGTRLIVN+Q
Sbjct: 361 RSTQVKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQ 420

Query: 421 KLQKDPLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTL 467
           KLQKDP VWE+P +F PERF+TSQKNFDVRG SPQ IPFGNGRRMCP ISFAL++MH+TL
Sbjct: 421 KLQKDPRVWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITL 480

BLAST of HG10014065 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 5.2e-180
Identity = 338/522 (64.75%), Postives = 380/522 (72.80%), Query Frame = 0

Query: 7   VAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMA 66
           +AGII AL+FF Y++    RRS  HRKKLPPE  GAWPVIGHLHLL+  EPTHITL+K+A
Sbjct: 6   LAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHITLSKLA 65

Query: 67  DAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPY 126
           D YGPIFT RFGMKKALIVSNWEIAKE FTT DRIFASRPKL AS LLGYDYAMMG SPY
Sbjct: 66  DKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPY 125

Query: 127 TPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAV 186
           +PHWRHARKIATLELLTNHRLEQ QHIRA EV++W  +LYEL +K        KDEK+ V
Sbjct: 126 SPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVK-------RKDEKLVV 185

Query: 187 EMKKKLADVTMNMIFKMVIGKRFSNMEDGSD-QKFQKALMNFFGLFSIFLPADSFPFLSW 246
           EMK   AD+T+N IF MVIGKRFS   DG D +  QKAL + F LF +F+P+DSFPFL W
Sbjct: 186 EMKNLFADITLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWW 245

Query: 247 LDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQ 306
           LD GGH++ MKKT KI+D  F+KFLKEHR R +  R ++EMKE DFMDVMIS V ++DG 
Sbjct: 246 LDFGGHQKRMKKTAKILDGAFEKFLKEHRQRRD--RVKLEMKEEDFMDVMISRV-NEDGN 305

Query: 307 HFSYDDDTVIKATCL------------------------------AQLELDNQVGRERHV 366
            FS+D DTV+KATCL                              AQLELD QVGRER V
Sbjct: 306 LFSFDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQV 365

Query: 367 KESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKD 426
           +ESDVKNL YLQ                           AGY+IP GTRLIV+ QKLQKD
Sbjct: 366 QESDVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKD 425

Query: 427 PLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLH 467
           P  WE+P +FRP+RFLTSQKN DVRGQSPQLIPFGNGRRMCPGISFAL++MHLTL N LH
Sbjct: 426 PSFWENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLH 485

BLAST of HG10014065 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 8.8e-180
Identity = 338/521 (64.88%), Postives = 379/521 (72.74%), Query Frame = 0

Query: 7   VAGIILALVFFFYSI----RRSVGHRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMA 66
           +AGII AL+FF Y++    RRS  HRKKLPPE  GAWPVIGHLHLL+  EPTHITL+K+A
Sbjct: 6   LAGIIFALLFFLYALFTISRRSAAHRKKLPPECGGAWPVIGHLHLLSTKEPTHITLSKLA 65

Query: 67  DAYGPIFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPY 126
           D YGPIFT RFGMKKALIVSNWEIAKE FTT DRIFASRPKL AS LLGYDYAMMG SPY
Sbjct: 66  DKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMGLSPY 125

Query: 127 TPHWRHARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAV 186
           +PHWRHARKIATLELLTNHRLEQ QHIRA EV++W  +LYEL +K        KDEK+ V
Sbjct: 126 SPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVK-------RKDEKLVV 185

Query: 187 EMKKKLADVTMNMIFKMVIGKRFSNMEDGSD-QKFQKALMNFFGLFSIFLPADSFPFLSW 246
           EMK   AD+T+N IF MVIGKRFS   DG D +  QKAL + F LF +F+P+DSFPFL W
Sbjct: 186 EMKNLFADITLNTIFMMVIGKRFSTAFDGHDGEDCQKALRDVFELFGMFVPSDSFPFLWW 245

Query: 247 LDLGGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQ 306
           LD GGH++ MKKT KI+D  F+KFLKEHR R +  R ++EMKE DFMDVMIS V ++DG 
Sbjct: 246 LDFGGHQKRMKKTAKILDGAFEKFLKEHRQRRD--RVKLEMKEEDFMDVMISRV-NEDGN 305

Query: 307 HFSYDDDTVIKATCL------------------------------AQLELDNQVGRERHV 366
            FS+D DTV+KATCL                              AQLELD QVGRER V
Sbjct: 306 LFSFDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQV 365

Query: 367 KESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKD 426
           +ESDVKNL YLQ                           AGY+IP GTRLIV+ QKLQKD
Sbjct: 366 QESDVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKD 425

Query: 427 PLVWEDPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLH 466
           P  WE+P +FRP+RFLTSQKN DVRGQSPQLIPFGNGRRMCPGISFAL++MHLTL N LH
Sbjct: 426 PSFWENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLH 485

BLAST of HG10014065 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-103
Identity = 208/521 (39.92%), Postives = 309/521 (59.31%), Query Frame = 0

Query: 14  LVFFFYSI-RRSVGHRKKLPPEASGAWPVIGHLHLLNANEP-THITLAKMADAYGPIFTL 73
           LVF F ++ ++S   +    P  SGAWP+IGHLHLL   E   + TL KMAD YGP  +L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 74  RFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHARK 133
           + G  +A +VS++E+AK+ FT ND+  ASRP   A+K +GY++A+ GF+PY+  WR  RK
Sbjct: 73  QLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRK 132

Query: 134 IATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLADV 193
           IAT+ELL+N RL+  +H+R  E+   +K+LY L  KN  +K       + V++K  L D+
Sbjct: 133 IATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTK------PVMVDLKSWLEDM 192

Query: 194 TMNMIFKMVIGKRF-------SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWLDL 253
           T+NMI +MV GKR+       S+ +     + +KA+  FF L  IF  +D+FP LS+ DL
Sbjct: 193 TLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDL 252

Query: 254 GGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEH--DFMDVMISAVKDDDGQH 313
            GHE+ MK+T   +D + +++++ HR +    R+    KE+  DF+DVM+S  +     H
Sbjct: 253 QGHEKEMKQTGSELDVILERWIENHRQQ----RKFSGTKENDSDFIDVMMSLAEQGKLSH 312

Query: 314 FSYDDDTVIKATCL------------------------------AQLELDNQVGRERHVK 373
             YD +T IK+TCL                              AQ E+D  VGR+R+V+
Sbjct: 313 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 372

Query: 374 ESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKDP 433
           +SD++NL YLQ                           AGY++P GTRLIVN+ K+Q+DP
Sbjct: 373 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 432

Query: 434 LVWEDPCKFRPERFLTSQ-KNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLH 466
            V+ +P +FRPERF+T + K FDVRGQ+ +L+PFG+GRR CPG S A++++HL LA FLH
Sbjct: 433 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 492

BLAST of HG10014065 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-101
Identity = 204/521 (39.16%), Postives = 306/521 (58.73%), Query Frame = 0

Query: 14  LVFFFYSI-RRSVGHRKKLPPEASGAWPVIGHLHLLNANEP-THITLAKMADAYGPIFTL 73
           LVF F ++ ++S   +    P  SGAWP+IGHLHLL+  E   + TL KMAD YGP  +L
Sbjct: 13  LVFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSL 72

Query: 74  RFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHARK 133
           R G  +  +VS++E+AK+ FT ND+  ASRP   A+K +GYD A+ GF+PY+  WR  RK
Sbjct: 73  RLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRK 132

Query: 134 IATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLADV 193
           IATLELL+N RL+  +H+R  E+   M++LY L +K   S      E + V++K  L D+
Sbjct: 133 IATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGS------EPVMVDLKSWLEDM 192

Query: 194 TMNMIFKMVIGKRF-----SNMEDGSD-QKFQKALMNFFGLFSIFLPADSFPFLSWLDLG 253
           ++NM+ +MV GKR+      + ED  + ++ +K + NFF L  IF  +D+FP L W D  
Sbjct: 193 SLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQ 252

Query: 254 GHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEH---DFMDVMISAVKDDDGQH 313
           GHE+ MK+T + +D + +++++ HR      +R+V   +H   DF+DVM+S  +     H
Sbjct: 253 GHEKEMKQTGRELDVILERWIENHRQ-----QRKVSGTKHNDSDFVDVMLSLAEQGKFSH 312

Query: 314 FSYDDDTVIKATCL------------------------------AQLELDNQVGRERHVK 373
             +D  T IK+TCL                              AQ E+D  VGR+R+V+
Sbjct: 313 LQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVE 372

Query: 374 ESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKDP 433
           +SD++NL Y+Q                           AGY++ +GTR++VN+ K+Q+DP
Sbjct: 373 DSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDP 432

Query: 434 LVWEDPCKFRPERFLTSQ-KNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLH 466
            V+ +P +FRPERF+T + K FDVRGQ+ +L+PFG+GRR CPG S A++++HL LA FL 
Sbjct: 433 RVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQ 492

BLAST of HG10014065 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 336.7 bits (862), Expect = 3.1e-92
Identity = 199/522 (38.12%), Postives = 291/522 (55.75%), Query Frame = 0

Query: 14  LVFFFYSI-RRSVGHRKKLPPEASGAWPVIGHLHLLNANEP-THITLAKMADAYGPIFTL 73
           LVF F ++ ++S   +    P  SGAWP+IGHLHLL   E   + TL KMAD YGP  +L
Sbjct: 13  LVFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSL 72

Query: 74  RFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHARK 133
           R G  +  + S++E+AK+ FT ND+  AS     A+K +GY +           W   RK
Sbjct: 73  RLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF-----------WLEMRK 132

Query: 134 IATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLADV 193
           IA +ELL+N RL+   ++R  E+   +K+LY L +K   S      E + V++K  L D+
Sbjct: 133 IAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGS------EPVMVDLKSWLEDM 192

Query: 194 TMNMIFKMVIGKRF-------SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWLDL 253
             NMI +MV GKR+       S+      ++++K +  FF L  IF  +D+FP L WLDL
Sbjct: 193 IANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDL 252

Query: 254 GGHERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEH---DFMDVMISAVKDDDGQ 313
            GHE+ MK+T + +D + +++++ HR      +R+V   +H   DF+DVM+S  +     
Sbjct: 253 QGHEKEMKQTRRELDVILERWIENHRQ-----QRKVSGTKHNDSDFVDVMLSLAEQGKLS 312

Query: 314 HFSYDDDTVIKATCLA------------------------------QLELDNQVGRERHV 373
           H  YD +T IK TCLA                              Q E+D  VGR+R+V
Sbjct: 313 HLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNV 372

Query: 374 KESDVKNLSYLQ---------------------------AGYHIPKGTRLIVNIQKLQKD 433
           ++SD+KNL YLQ                           AGY++P GTRLIVN+ K+Q+D
Sbjct: 373 EDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRD 432

Query: 434 PLVWEDPCKFRPERFLTSQ-KNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFL 466
           P V+ +P +FRPERF+T + K+FDVRGQ+ +L+PFG+GRR CPG S A++++HL LA FL
Sbjct: 433 PKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFL 492

BLAST of HG10014065 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 302.8 bits (774), Expect = 4.9e-82
Identity = 178/503 (35.39%), Postives = 267/503 (53.08%), Query Frame = 0

Query: 27  HRKKLPPEASGAWPVIGHLHLLNANEPTHITLAKMADAYGPIFTLRFGMKKALIVSNWEI 86
           ++K   P   GAWP++GHLHL +   PTH+T   MAD YGP+F  + G  K +I+++ E+
Sbjct: 29  NKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVFMAKLGSIKVMIINSKEV 88

Query: 87  AKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWRHARKIATLELLTNHRLEQF 146
           AKE +T +D++   RP+L ASKLLGY+ + + FSPY  +WR  RKIA  EL +   ++  
Sbjct: 89  AKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLYWREIRKIAVSELFSTSGVDMH 148

Query: 147 QHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKKLADVTMNMIFKMVIGKRF- 206
              RA E     + LY         K     E + V+MK++  D+T N+   MV GKR+ 
Sbjct: 149 MVSRAREADLAFRALYV-----RWEKRGKPKEGVLVDMKQEFIDLTANISLMMVSGKRYF 208

Query: 207 ---SNMEDGSDQKFQKALMNFFGLFSIFLPADSFPFLSWLDLGGHERVMKKTMKIIDNVF 266
               N E    ++  K +  F   F++FL +D  P L +LD    +R MK+T K +D V 
Sbjct: 209 GENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-WKTKRGMKRTAKGLDKVA 268

Query: 267 DKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQHFSYDDDTVIKATCL------ 326
           + +++EH+++ ++  R     E+D++D++I  +  D     S D  T IKA CL      
Sbjct: 269 EGWIEEHKNKRSDHGR----SENDYLDILIKILGQDKIPGLS-DTHTKIKALCLNLVLAG 328

Query: 327 ------------------------AQLELDNQVGRERHVKESDVKNLSYLQA-------- 386
                                   AQ ELD+++G+ER V+E D+K+L YLQA        
Sbjct: 329 SETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRL 388

Query: 387 ---------------------GYHIPKGTRLIVNIQKLQKDPLVWEDPCKFRPERFLTSQ 446
                                  H+P GT+L+V+  K+ +DP VW +P +F PERFLTS 
Sbjct: 389 YPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSN 448

Query: 447 KNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLHGFIISRPSEELLDMEESDG 467
           +  DV GQS +  PFG GRR CP I   ++++H  L  FLH F ++RPS + +DM ES+G
Sbjct: 449 RELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPSSQDVDMTESNG 508

BLAST of HG10014065 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 270.4 bits (690), Expect = 2.7e-72
Identity = 172/516 (33.33%), Postives = 264/516 (51.16%), Query Frame = 0

Query: 12  LALVFFFYSIRRSVGHRKKLP----PEASGAWPVIGHLHLLNANEPTHITLAKMADAYGP 71
           LALV F Y   R    R ++     PE  GA P+ GHLHLL   +     LA M+  +GP
Sbjct: 15  LALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGP 74

Query: 72  IFTLRFGMKKALIVSNWEIAKEFFTTNDRIFASRPKLVASKLLGYDYAMMGFSPYTPHWR 131
           IF+L+ G  + ++ S+ +  K+ FTTND   A+RP +   + +GY+ A +  +PY  +WR
Sbjct: 75  IFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWR 134

Query: 132 HARKIATLELLTNHRLEQFQHIRAFEVKKWMKELYELCLKNSCSKNNNKDEKMAVEMKKK 191
             RKI T+ L +NH +E   HIR+ EV   +K LY         K N     + ++M  +
Sbjct: 135 ELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY---------KGNGGTSIVKIDMLFE 194

Query: 192 LADVTMNMIFKMVIGKRFSNMEDGSDQ-KFQKALMNFFGLFSIFLPADSFPFLSWLDLGG 251
              +T N+I + ++GKR    E  SD+ ++++AL +   L  I +  D  P+L WLD   
Sbjct: 195 F--LTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAK 254

Query: 252 HERVMKKTMKIIDNVFDKFLKEHRDRTNNCRREVEMKEHDFMDVMISAVKDDDGQHFSYD 311
           + + MK+  K +D+V  K+L EH  + +   R  + +E   MD+++  +  +D     + 
Sbjct: 255 NSQ-MKRLFKELDSVNTKWLHEHLKKRS---RNEKDQERTIMDLLLD-ILPEDIVISGHV 314

Query: 312 DDTVIKATCL------------------------------AQLELDNQVGRERHVKESDV 371
            D ++KAT L                              AQ E+DN VG+ R ++ESD+
Sbjct: 315 RDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDI 374

Query: 372 KNLSYLQA---------------------------GYHIPKGTRLIVNIQKLQKDPLVWE 431
           +NL YLQA                           GY + KGTRL+VNI KL +DP +W 
Sbjct: 375 QNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWP 434

Query: 432 DPCKFRPERFLTSQKNFDVRGQSPQLIPFGNGRRMCPGISFALKIMHLTLANFLHGFIIS 466
           DP  F+PERF+  +   +    + + IPFG+GRR CPG++  L+++H  LA  L GF + 
Sbjct: 435 DPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELH 494