HG10014052 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014052
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Calmodulin
Location	Chr02: 7173229 .. 7173771 (+)
RNA-Seq Expression	HG10014052
Synteny	HG10014052

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTGATCAATTTCCCTCTCCTTCACTCCAAACATAAACCAACGTTGTCGTCGTCCAAACCTCCTCCGCCGCCCGTCGCCGAAGTTGCCTTTCGCCACCTCGACGCTGATGGAGATGGAAAAATATCTAGCGATGAGCTTCGATCGTACTTCGCATCGATCGGTGAGTACATGACACGCGAAGATGCTCGAAGCGTGATTGGAGATTTTGATGGAGATGGCGATGAGCTACTAGAGCTCGAAGATTTCGAACGACTTGTAAAAGGAGAGGAGGCAGAGGAGGAAGAGAAAGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAAAAGGGATGTGGATTCATTGGACCGGCAGGGTTGCAAAAGATGTTTGGGCGGCTTGGGTACGTGAAATCAAAAGAAGAGTGCATGGCTATGATCAGAGTGTTTGATGTTGACGGTGATGGGGTAATTGATTATCGTGAATTTCTCAGAATGATGACTTGA

mRNA sequence

ATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTGATCAATTTCCCTCTCCTTCACTCCAAACATAAACCAACGTTGTCGTCGTCCAAACCTCCTCCGCCGCCCGTCGCCGAAGTTGCCTTTCGCCACCTCGACGCTGATGGAGATGGAAAAATATCTAGCGATGAGCTTCGATCGTACTTCGCATCGATCGGTGAGTACATGACACGCGAAGATGCTCGAAGCGTGATTGGAGATTTTGATGGAGATGGCGATGAGCTACTAGAGCTCGAAGATTTCGAACGACTTGTAAAAGGAGAGGAGGCAGAGGAGGAAGAGAAAGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAAAAGGGATGTGGATTCATTGGACCGGCAGGGTTGCAAAAGATGTTTGGGCGGCTTGGGTACGTGAAATCAAAAGAAGAGTGCATGGCTATGATCAGAGTGTTTGATGTTGACGGTGATGGGGTAATTGATTATCGTGAATTTCTCAGAATGATGACTTGA

Coding sequence (CDS)

ATGGCCATTTCAACCATTTCCATTCCCTTAAAATGGTTTTCCAAAACCAATTTCAAGCTGATCAATTTCCCTCTCCTTCACTCCAAACATAAACCAACGTTGTCGTCGTCCAAACCTCCTCCGCCGCCCGTCGCCGAAGTTGCCTTTCGCCACCTCGACGCTGATGGAGATGGAAAAATATCTAGCGATGAGCTTCGATCGTACTTCGCATCGATCGGTGAGTACATGACACGCGAAGATGCTCGAAGCGTGATTGGAGATTTTGATGGAGATGGCGATGAGCTACTAGAGCTCGAAGATTTCGAACGACTTGTAAAAGGAGAGGAGGCAGAGGAGGAAGAGAAAGAGGATTTGAAAAGGGCTTTTGAGATGTTTGAAGGTGAAAAGGGATGTGGATTCATTGGACCGGCAGGGTTGCAAAAGATGTTTGGGCGGCTTGGGTACGTGAAATCAAAAGAAGAGTGCATGGCTATGATCAGAGTGTTTGATGTTGACGGTGATGGGGTAATTGATTATCGTGAATTTCTCAGAATGATGACTTGA

Protein sequence

MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPPPPVAEVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFLRMMT

Homology

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BLAST of HG10014052 vs. NCBI nr
Match: XP_008466624.1 (PREDICTED: probable calcium-binding protein CML41 [Cucumis melo] >KAA0055432.1 putative calcium-binding protein CML41 [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 4.3e-76
Identity = 152/180 (84.44%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPPPPVAEVAFRHLDADGDGKI 60
           MAISTI+IP KWF+KTNFKL NFPLLHSKHKP +SS  P PPP+AEVAFRHLD DGDGKI
Sbjct: 1   MAISTIAIPFKWFTKTNFKL-NFPLLHSKHKP-ISSKPPTPPPLAEVAFRHLDIDGDGKI 60

Query: 61  SSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKEDLKR 120
           S DELRSYFASIGEYMT + A+SVIGDFD DGDELLEL DFERLVKGE  EEE++EDLKR
Sbjct: 61  SGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGE--EEEQEEDLKR 120

Query: 121 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFLRMMT 180
           AFEMFEGEKGCGFIG  GLQKMFGRLGYVKSKE+CM MI+VFDVDGDGVIDY EFLRMMT
Sbjct: 121 AFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMMT 176

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BLAST of HG10014052 vs. NCBI nr
Match: XP_038905487.1 (probable calcium-binding protein CML41 [Benincasa hispida])

HSP 1 Score: 291.6 bits (745), Expect = 4.7e-75
Identity = 153/179 (85.47%), Postives = 161/179 (89.94%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPPPPVAEVAFRHLDADGDGKI 60
           MAISTISIPLKWFS      +NFP LHSKHKPT  SSKPP  P+AEVAFRHLD DGDGKI
Sbjct: 1   MAISTISIPLKWFSTK----LNFPRLHSKHKPT--SSKPPSLPLAEVAFRHLDVDGDGKI 60

Query: 61  SSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKEDLKR 120
           SSDELR+YFASIGEYMTR+ A+SVIGDFD DGDELLEL DF+RLVKGE  EEEE+EDLKR
Sbjct: 61  SSDELRTYFASIGEYMTRDGAQSVIGDFDEDGDELLELGDFQRLVKGE--EEEEEEDLKR 120

Query: 121 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFLRMM 180
           AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEEC AMI+VFDVDGDGVIDYREFLRMM
Sbjct: 121 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECSAMIKVFDVDGDGVIDYREFLRMM 171

[Close]

BLAST of HG10014052 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.9 bits (725), Expect = 9.8e-73
Identity = 149/187 (79.68%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPP-------PPVAEVAFRHLD 60
           MA+S IS P KWFS TN KL N P L  KHKPT S+  PPP       PP+AE AFRHLD
Sbjct: 1   MALSPISTPFKWFSNTNLKL-NLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLD 60

Query: 61  ADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEE 120
           ADGDGKIS DEL+SYFAS+GEYMTR+DARS+I +FDGDGD LLEL DFERLVKG   EEE
Sbjct: 61  ADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG--GEEE 120

Query: 121 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYR 180
           EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDYR
Sbjct: 121 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYR 180

[Close]

BLAST of HG10014052 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 283.9 bits (725), Expect = 9.8e-73
Identity = 149/187 (79.68%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPP-------PPVAEVAFRHLD 60
           MA+S IS P KWFS TNFKL NFP L  KHKPT S+  PPP       PP+AE AFRHLD
Sbjct: 15  MALSPISTPFKWFSNTNFKL-NFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLD 74

Query: 61  ADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEE 120
           ADGDGKIS DEL+SYFAS+GEYMTR+DARS+I +FDGDGD LLEL DFERLVKG   EEE
Sbjct: 75  ADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG--GEEE 134

Query: 121 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYR 180
           EKEDLK AFEMFEGEKGCGFIGP GLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDYR
Sbjct: 135 EKEDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYR 194

[Close]

BLAST of HG10014052 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-72
Identity = 148/187 (79.14%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPP-------PPVAEVAFRHLD 60
           MA+S +S P KWFS TNF L NFP L  KHKPT S+  PPP       PP+AE AFRHLD
Sbjct: 38  MALSPVSTPFKWFSNTNFNL-NFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLD 97

Query: 61  ADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEE 120
           ADGDGKIS DEL+SYFAS+GEYMTR+DARS+I +FD DGD LLEL DFERLVKG   EEE
Sbjct: 98  ADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKG--GEEE 157

Query: 121 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYR 180
           EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDYR
Sbjct: 158 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYR 217

[Close]

BLAST of HG10014052 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-28
Identity = 78/192 (40.62%), Postives = 106/192 (55.21%), Query Frame = 0


Query: 11  KWFSKTNFKL------------------INFPLLHSKHKPTLSSSKPPPPPVAEVAFRHL 70
           KWFS    KL                  +N P  +S     + S +     + +V F H 
Sbjct: 14  KWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQV-FSHF 73

Query: 71  DADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGE---- 130
           D+DGDGKIS+ ELR YF S+GEY++ E A+  I + D D D  L  EDF  L+       
Sbjct: 74  DSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYG 133

Query: 131 EAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDG 181
           + E +   +LK AFEMFE EKG G I P GLQKM  +LG  ++  EC AMI+ +D+DG+G
Sbjct: 134 DGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNG 193

[Close]

BLAST of HG10014052 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 5.0e-19
Identity = 52/136 (38.24%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 46  EVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLV 105
           E  F ++DA+ DG+IS++EL+  F ++GE M+ E+A + +   D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 106 KG--EEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFD 165
           KG  E  EEE+K  +  AF M+  + G   I P  L+ M  +LG  ++ ++C  MI+ FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIAD-GEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 166 VDGDGVIDYREFLRMM 180
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of HG10014052 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 6.5e-19
Identity = 52/134 (38.81%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 46  EVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLV 105
           E  F ++DA+ DG+IS +EL+  F ++GE ++ E+A + +   D DGD +L+ E+F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 106 KGEEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVD 165
           K ++ EEE+K +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++
Sbjct: 105 KVDD-EEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 164

Query: 166 GDGVIDYREFLRMM 180
            DGV+ + EF  MM
Sbjct: 165 ADGVLSFDEFALMM 176

[Close]

BLAST of HG10014052 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.5e-18
Identity = 62/134 (46.27%), Postives = 83/134 (61.94%), Query Frame = 0


Query: 49  FRHLDADGDGKISSDELRSYF-ASIGEYMTREDARSVIGDFDGDGDELLELEDFERL-VK 108
           F   D D DGKIS+ ELR    A++GE M+ E+A +++   D D D LL+ E+F +L V+
Sbjct: 12  FSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTKLAVQ 71

Query: 109 GEEAEEEEK-EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVD 168
            E  +EEE+   L  AF M+E E G G I PA L++M  +LG  +  EEC  MI  FD+D
Sbjct: 72  LEMGDEEERCRGLMEAFRMYEME-GEGRITPASLKRMLSKLGSHQGIEECQTMICRFDLD 131

Query: 169 GDGVIDYREFLRMM 180
           GDGVI + EF  MM
Sbjct: 132 GDGVISFEEFKIMM 144

[Close]

BLAST of HG10014052 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 2.5e-18
Identity = 65/182 (35.71%), Postives = 96/182 (52.75%), Query Frame = 0


Query: 9   PLKWFSKTNFKLINFPLLHSKHKPTLSSSKPP----PPPVAEVAFRHLDADGDGKISSDE 68
           PL WF KT  K  N P   S   P+ SSS  P     P   +  F+  D +GDG+I+ +E
Sbjct: 28  PLSWFDKTLHK--NSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 87

Query: 69  LRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAE-----EEEKEDLK 128
           L     ++G Y+  +D   +I   D +GD  +++++FE L      E     E E+ED+K
Sbjct: 88  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMK 147

Query: 129 RAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSK--EECMAMIRVFDVDGDGVIDYREFLR 180
            AF +F+ + G GFI    L+ +   LG  + K  + C  MI   D DGDG ++Y+EFL+
Sbjct: 148 DAFNVFD-QDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQ 206

[Close]

BLAST of HG10014052 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-76
Identity = 152/180 (84.44%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPPPPVAEVAFRHLDADGDGKI 60
           MAISTI+IP KWF+KTNFKL NFPLLHSKHKP +SS  P PPP+AEVAFRHLD DGDGKI
Sbjct: 1   MAISTIAIPFKWFTKTNFKL-NFPLLHSKHKP-ISSKPPTPPPLAEVAFRHLDIDGDGKI 60

Query: 61  SSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKEDLKR 120
           S DELRSYFASIGEYMT + A+SVIGDFD DGDELLEL DFERLVKGE  EEE++EDLKR
Sbjct: 61  SGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGE--EEEQEEDLKR 120

Query: 121 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFLRMMT 180
           AFEMFEGEKGCGFIG  GLQKMFGRLGYVKSKE+CM MI+VFDVDGDGVIDY EFLRMMT
Sbjct: 121 AFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMMT 176

[Close]

BLAST of HG10014052 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-76
Identity = 152/180 (84.44%), Postives = 162/180 (90.00%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPPPPVAEVAFRHLDADGDGKI 60
           MAISTI+IP KWF+KTNFKL NFPLLHSKHKP +SS  P PPP+AEVAFRHLD DGDGKI
Sbjct: 1   MAISTIAIPFKWFTKTNFKL-NFPLLHSKHKP-ISSKPPTPPPLAEVAFRHLDIDGDGKI 60

Query: 61  SSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKEDLKR 120
           S DELRSYFASIGEYMT + A+SVIGDFD DGDELLEL DFERLVKGE  EEE++EDLKR
Sbjct: 61  SGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGE--EEEQEEDLKR 120

Query: 121 AFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFLRMMT 180
           AFEMFEGEKGCGFIG  GLQKMFGRLGYVKSKE+CM MI+VFDVDGDGVIDY EFLRMMT
Sbjct: 121 AFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMMT 176

[Close]

BLAST of HG10014052 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 4.7e-73
Identity = 149/187 (79.68%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPP-------PPVAEVAFRHLD 60
           MA+S IS P KWFS TNFKL NFP L  KHKPT S+  PPP       PP+AE AFRHLD
Sbjct: 15  MALSPISTPFKWFSNTNFKL-NFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLD 74

Query: 61  ADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEE 120
           ADGDGKIS DEL+SYFAS+GEYMTR+DARS+I +FDGDGD LLEL DFERLVKG   EEE
Sbjct: 75  ADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG--GEEE 134

Query: 121 EKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYR 180
           EKEDLK AFEMFEGEKGCGFIGP GLQKMFGRLGYVKS EECMAMI VFDVDGDGVIDYR
Sbjct: 135 EKEDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYR 194

[Close]

BLAST of HG10014052 vs. ExPASy TrEMBL
Match: A0A0A0LG17 (Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 8.1e-73
Identity = 151/184 (82.07%), Postives = 161/184 (87.50%), Query Frame = 0


Query: 1   MAISTIS-IPLKWFSKTNFKLINFPLLHSKHKPTL---SSSKPPPPPVAEVAFRHLDADG 60
           MAIS+I+ IP KWF+KTNFKL NFPLLHSKHKPT    S+S P P P+AEVAFRHLD DG
Sbjct: 1   MAISSIAVIPFKWFTKTNFKL-NFPLLHSKHKPTSSKPSTSGPGPRPLAEVAFRHLDVDG 60

Query: 61  DGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEEKE 120
           DGKIS +EL+SYFASIGEYMT  DARSVIGDFD DGDELLEL DFER+VKG   EEEE+E
Sbjct: 61  DGKISCNELQSYFASIGEYMTWNDARSVIGDFDRDGDELLELGDFERMVKG---EEEEEE 120

Query: 121 DLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYREFL 180
           DLKRAFEMFEGEKGCGFI   GLQKMFGRLGYVKSKEECMAMI+VFDVDGDGVIDY EFL
Sbjct: 121 DLKRAFEMFEGEKGCGFIEATGLQKMFGRLGYVKSKEECMAMIKVFDVDGDGVIDYHEFL 180

[Close]

BLAST of HG10014052 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 3.1e-72
Identity = 147/186 (79.03%), Postives = 157/186 (84.41%), Query Frame = 0


Query: 1   MAISTISIPLKWFSKTNFKLINFPLLHSKHKPTLSSSKPPP------PPVAEVAFRHLDA 60
           MA+S IS P KWFS TN KL N P L  KHKPT S+  PPP      PP+AE AFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKL-NLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAEEEE 120
           DGDGKIS DEL+SYFAS+GEYMTR+DARS+I +FDGDGD LLEL DFERLVKG   E+EE
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG--GEQEE 120

Query: 121 KEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDGVIDYRE 180
           KEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKS EECMAMI VFDVDG+GVIDYRE
Sbjct: 121 KEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYRE 180

[Close]

BLAST of HG10014052 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 78/192 (40.62%), Postives = 106/192 (55.21%), Query Frame = 0


Query: 11  KWFSKTNFKL------------------INFPLLHSKHKPTLSSSKPPPPPVAEVAFRHL 70
           KWFS    KL                  +N P  +S     + S +     + +V F H 
Sbjct: 14  KWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQV-FSHF 73

Query: 71  DADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGE---- 130
           D+DGDGKIS+ ELR YF S+GEY++ E A+  I + D D D  L  EDF  L+       
Sbjct: 74  DSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYG 133

Query: 131 EAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVDGDG 181
           + E +   +LK AFEMFE EKG G I P GLQKM  +LG  ++  EC AMI+ +D+DG+G
Sbjct: 134 DGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNG 193

[Close]

BLAST of HG10014052 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 95.9 bits (237), Expect = 3.5e-20
Identity = 52/136 (38.24%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 46  EVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLV 105
           E  F ++DA+ DG+IS++EL+  F ++GE M+ E+A + +   D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 106 KG--EEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFD 165
           KG  E  EEE+K  +  AF M+  + G   I P  L+ M  +LG  ++ ++C  MI+ FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIAD-GEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 166 VDGDGVIDYREFLRMM 180
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

[Close]

BLAST of HG10014052 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 95.5 bits (236), Expect = 4.6e-20
Identity = 52/134 (38.81%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 46  EVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLV 105
           E  F ++DA+ DG+IS +EL+  F ++GE ++ E+A + +   D DGD +L+ E+F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 106 KGEEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVD 165
           K ++ EEE+K +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++
Sbjct: 105 KVDD-EEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 164

Query: 166 GDGVIDYREFLRMM 180
            DGV+ + EF  MM
Sbjct: 165 ADGVLSFDEFALMM 176

[Close]

BLAST of HG10014052 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 95.5 bits (236), Expect = 4.6e-20
Identity = 52/134 (38.81%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 46  EVAFRHLDADGDGKISSDELRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLV 105
           E  F ++DA+ DG+IS +EL+  F ++GE ++ E+A + +   D DGD +L+ E+F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 106 KGEEAEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSKEECMAMIRVFDVD 165
           K ++ EEE+K +LK AF ++  E G   I P  L+ M  +LG  ++ ++C  MI  FD++
Sbjct: 105 KVDD-EEEKKMELKGAFRLYIAE-GEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLN 164

Query: 166 GDGVIDYREFLRMM 180
            DGV+ + EF  MM
Sbjct: 165 ADGVLSFDEFALMM 176

[Close]

BLAST of HG10014052 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.7e-19
Identity = 65/182 (35.71%), Postives = 96/182 (52.75%), Query Frame = 0


Query: 9   PLKWFSKTNFKLINFPLLHSKHKPTLSSSKPP----PPPVAEVAFRHLDADGDGKISSDE 68
           PL WF KT  K  N P   S   P+ SSS  P     P   +  F+  D +GDG+I+ +E
Sbjct: 28  PLSWFDKTLHK--NSPPSPSTMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEE 87

Query: 69  LRSYFASIGEYMTREDARSVIGDFDGDGDELLELEDFERLVKGEEAE-----EEEKEDLK 128
           L     ++G Y+  +D   +I   D +GD  +++++FE L      E     E E+ED+K
Sbjct: 88  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMK 147

Query: 129 RAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSK--EECMAMIRVFDVDGDGVIDYREFLR 180
            AF +F+ + G GFI    L+ +   LG  + K  + C  MI   D DGDG ++Y+EFL+
Sbjct: 148 DAFNVFD-QDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQ 206

The following BLAST results are available for this feature:

BLAST of HG10014052 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466624.1	4.3e-76	84.44	PREDICTED: probable calcium-binding protein CML41 [Cucumis melo] >KAA0055432.1 p...	[more]
XP_038905487.1	4.7e-75	85.47	probable calcium-binding protein CML41 [Benincasa hispida]	[more]
KAG6591338.1	9.8e-73	79.68	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022974919.1	9.8e-73	79.68	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
XP_023535419.1	1.7e-72	79.14	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10014052 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	1.5e-28	40.63	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SRE7	5.0e-19	38.24	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]
Q9SRE6	6.5e-19	38.81	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYJ8	2.5e-18	46.27	Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O22845	2.5e-18	35.71	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]

BLAST of HG10014052 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UHX6	2.1e-76	84.44	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	2.1e-76	84.44	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]
A0A6J1IIY2	4.7e-73	79.68	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A0A0LG17	8.1e-73	82.07	Calmodulin OS=Cucumis sativus OX=3659 GN=Csa_3G854250 PE=4 SV=1	[more]
A0A6J1F7Y7	3.1e-72	79.03	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of HG10014052 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	1.1e-29	40.63	calmodulin-like 41	[more]
AT1G76640.1	3.5e-20	38.24	Calcium-binding EF-hand family protein	[more]
AT1G76650.1	4.6e-20	38.81	calmodulin-like 38	[more]
AT1G76650.3	4.6e-20	38.81	calmodulin-like 38	[more]
AT2G43290.1	1.7e-19	35.71	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 98..128
None	No IPR available	GENE3D	1.10.238.10		coord: 108..180 e-value: 2.9E-17 score: 64.5
None	No IPR available	GENE3D	1.10.238.10		coord: 24..107 e-value: 6.7E-15 score: 57.5
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 45..180
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 45..180
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 154..180 e-value: 0.028 score: 23.6 coord: 44..72 e-value: 3.3E-4 score: 30.0 coord: 80..108 e-value: 11.0 score: 9.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 47..105 e-value: 2.1E-10 score: 40.9
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 132..179 e-value: 3.0E-8 score: 33.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 40..75 score: 12.979158
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..180 score: 15.378162
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 76..111 score: 8.376417
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..180 e-value: 1.8165E-10 score: 52.1649
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 45..106 e-value: 2.57134E-12 score: 57.1725
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 47..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014052.1	HG10014052.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity