HG10013914 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10013914
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA pseudouridine synthase A
Location	Chr02: 5997967 .. 5999628 (+)
RNA-Seq Expression	HG10013914
Synteny	HG10013914

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCAGACCCGAAGAAGCTCAAAATGTCATCCACAACCACCACCTCCGACGACGAAGGTGTCAATTCCAATTCTGAGGCGCCGAAGAAACAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGGGCAGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTTGTTTCTGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTTGAATCTTTCGCCTCAGATTCTAATTTTCGGGTACAAGCGCGTCACGGCGTCATTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTCTTCGCTCTCGATCCTAATTGCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTGTGATAACTGAGTCAGACATTTCTTCGAACACTGGAACCGCCATTGAAGTTTCTGATATTCAGGAAAATAGGGTTTTGGAGGACAATGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTCGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCAGTTACATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATAGGAATGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAGAATTATGTAGGAACTCACAATTTCCATAATTTTACTACCAGAACAAAAGCAGAGGACCCTGCTGCTCATCGGTTCATTGTTTCATTTGATGCGTCTAATACCGTCGTGGTTGAAGGCATCGAATTCGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCCTTGATTGAGAAGGCTTTGCAAAAGTAAGTATTTTCCATTTCCAACTACAATATGATTGGATTTTCATTTGTTAATAGCATAGAATTGGATTCCATCAACTTTTGGTAATCAAAATTCAAACACAGGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGACTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACAGTCATGAAGAAGTATCTATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGTGTTGTTGATGAGGTTGCAGAGATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGCTGAAACTTAA

mRNA sequence

ATGGAGAAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCAGACCCGAAGAAGCTCAAAATGTCATCCACAACCACCACCTCCGACGACGAAGGTGTCAATTCCAATTCTGAGGCGCCGAAGAAACAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGGGCAGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTTGTTTCTGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTTGAATCTTTCGCCTCAGATTCTAATTTTCGGGTACAAGCGCGTCACGGCGTCATTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTCTTCGCTCTCGATCCTAATTGCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTGTGATAACTGAGTCAGACATTTCTTCGAACACTGGAACCGCCATTGAAGTTTCTGATATTCAGGAAAATAGGGTTTTGGAGGACAATGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTCGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCAGTTACATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATAGGAATGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAGAATTATGTAGGAACTCACAATTTCCATAATTTTACTACCAGAACAAAAGCAGAGGACCCTGCTGCTCATCGGTTCATTGTTTCATTTGATGCGTCTAATACCGTCGTGGTTGAAGGCATCGAATTCGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCCTTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGACTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACAGTCATGAAGAAGTATCTATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGTGTTGTTGATGAGGTTGCAGAGATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGCTGAAACTTAA

Coding sequence (CDS)

ATGGAGAAAGAACCGCCAACTGAAACCAGAAGGTCACCGCCACAACCGTTGATCGAAGAACCAGACCCGAAGAAGCTCAAAATGTCATCCACAACCACCACCTCCGACGACGAAGGTGTCAATTCCAATTCTGAGGCGCCGAAGAAACAAAGGTACAAGCGCCGCAAGATCGCCATTTTCTTTGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCTAAAACCATTGAAGCTGACCTTGAAGAGGCCCTATATCTCGCCGGGGCAGTCCCGGAGCAGGACCGAGGGCTCTCGAAGCGCTACGATTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTGAGCGCCGTGGGTCAGGTTGTTTCTGGTCGCTTCTACATCGATCCGCCAGGTTTTGTTGATCGTCTCAATTTGAATCTTTCGCCTCAGATTCTAATTTTCGGGTACAAGCGCGTCACGGCGTCATTTAATGCGAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCTGTCTTCGCTCTCGATCCTAATTGCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGACAACGAGCTTGTGAAGTGCTTAGAGTGCTCTGAACGAGGACGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGTAAAAACTGTGATAACTGAGTCAGACATTTCTTCGAACACTGGAACCGCCATTGAAGTTTCTGATATTCAGGAAAATAGGGTTTTGGAGGACAATGCCACGACCTCTGATTCTAACAAACAAACTAGCGTTTTAGTCGAAAATAGTTGTACTGAACTCAATGTTGGAAGCGAAGGTACTGCAAAAAGTGAAGGCAGTTACATGTCTGGATTAGGGAATGATTCAGAGATTGTTGTAAGCAAAGAGAGCGTTAATAGGAATGAAAAACCCTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTTTGAAGAATTATGTAGGAACTCACAATTTCCATAATTTTACTACCAGAACAAAAGCAGAGGACCCTGCTGCTCATCGGTTCATTGTTTCATTTGATGCGTCTAATACCGTCGTGGTTGAAGGCATCGAATTCGTGAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAAATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCCTTGATTGAGAAGGCTTTGCAAAAGGATGTAAATATCAATGTGCCCACTGCACCTGAGGTTGGACTGTACTTAGATGAGTGCCTATTCACATCATATAATCAAAAGTGGAAAGACAGTCATGAAGAAGTATCTATGAAAGCATACGAAGAGGTAGCAGAAGATTTCAAAATGAAGCAAATATATTCTCACATTGCTTCCACAGAGCACAAAGATGGAGCCGTGGCCTTATGGTTGCATTCTCTAAACCATCGGAATTATCCAGATTTACGTGTTGTTGATGAGGTTGCAGAGATTGCTAAGAACACAAGTGTTGCCATGGAGACACAAGCTGAAACTTAA

Protein sequence

MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTAIEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEIVVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET

Homology

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BLAST of HG10013914 vs. NCBI nr
Match: XP_038899088.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 964.9 bits (2493), Expect = 2.8e-277
Identity = 494/519 (95.18%), Postives = 499/519 (96.15%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETR SPPQPLIEEPDPKKLKMSSTTTTSDDE VNS+S APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRISPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSSSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT I ES ISSNTG A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAIIESHISSNTGNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+QENRVLEDNAT SDSNKQ+SVLVENSCTELNV SE TAKS GS MSGLGNDSEI
Sbjct: 241 IEVSDVQENRVLEDNATISDSNKQSSVLVENSCTELNVESESTAKSVGSEMSGLGNDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN NEKPSGSFKY EEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGNEKPSGSFKYCEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLR VDEVAEIA NTSVAMETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRAVDEVAEIAMNTSVAMETQAET 519

[Close]

BLAST of HG10013914 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo] >KAA0064620.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa] >TYK19972.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 958.0 bits (2475), Expect = 3.4e-275
Identity = 489/519 (94.22%), Postives = 498/519 (95.95%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT ITESDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TEL V SEG AKSE S MSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of HG10013914 vs. NCBI nr
Match: XP_004145528.1 (tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_012098 [Cucumis sativus])

HSP 1 Score: 950.7 bits (2456), Expect = 5.4e-273
Identity = 485/519 (93.45%), Postives = 497/519 (95.76%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT ITESDISSNT  A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TELNVGSEGTAKSE S MSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]

BLAST of HG10013914 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 934.5 bits (2414), Expect = 4.0e-268
Identity = 476/519 (91.71%), Postives = 490/519 (94.41%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDE VNSNS APKKQRYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT I+ESDIS N G A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N  LEDNAT SDS K+TSVLVENS TELNVGSE  AKSEGS MSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSK+SVNRNEKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAA RFI+S
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+ TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV DE A+ AKNTSV +ETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAET 519

[Close]

BLAST of HG10013914 vs. NCBI nr
Match: XP_022936288.1 (tRNA pseudouridine synthase A [Cucurbita moschata])

HSP 1 Score: 925.2 bits (2390), Expect = 2.4e-265
Identity = 473/520 (90.96%), Postives = 487/520 (93.65%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDE VNSNS APKKQRYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT I+ESDIS N+G A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNSGNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEV D+Q N  LEDNAT SDS K+TSVLVENS TELNVG E TAK EGS MSGLGNDSE 
Sbjct: 241 IEVPDVQANGALEDNATASDSIKETSVLVENSGTELNVGGEATAKIEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           V SKESVNRNEK SGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAA RFI+S
Sbjct: 301 VASKESVNRNEKSSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+ TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVA-EIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV DE A + AKNTSV ++TQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADDNAKNTSVGIDTQAET 520

[Close]

BLAST of HG10013914 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 1.8e-53
Identity = 156/485 (32.16%), Postives = 236/485 (48.66%), Query Frame = 0


Query: 4   EPPTETRRSPPQPLIEEPDPKKLKMS-------STTTTSDDEGVNSNSEAPKKQRYKRRK 63
           E  T  R+S P P   E D K+LK           T   D+ G       PK  R  +RK
Sbjct: 95  ESTTRVRKSSPSPSANENDKKRLKKEFQPRQKIEYTPLVDENG----QPIPKAPRKPKRK 154

Query: 64  IAIFFAYCGVGYQGM--QKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTD 123
           +A+   YCG GY GM  Q +P  KTIE D+ +A+  AGA+  ++    K+  + R+ARTD
Sbjct: 155 VAVMLGYCGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTD 214

Query: 124 KGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLI 183
           KGV A G V+S +  I+ P  ++++N  L  QI I+G +R T  F+ +K C  R Y YL+
Sbjct: 215 KGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLL 274

Query: 184 PVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDIS 243
           P F+L P         K+ L   +ELVK        +K+E                 D+ 
Sbjct: 275 PTFSLLP------PKPKSVL---SELVK-------EKKLE---------------NPDLF 334

Query: 244 SNTGTAIEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGL 303
            +    I+  +  ++++L    T    +  TS   E    +     +  A  E   +S L
Sbjct: 335 EDDQEGIDWWENVKSKILASGITQEQIDSITSSYDEQQ-QQQQQQQQQQADEEERDISEL 394

Query: 304 GNDSEIVVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAA 363
                I   K   N++ +   S++        F  ++K Y GTHNFHNFT     +D +A
Sbjct: 395 SFTKLIKQIKTIENQSRR---SYRISSSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSA 454

Query: 364 HRFIVSFDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ 423
           +RF++    S+  V+EG E++  ++ GQSFMLHQIRKM+ +A  ++R   P  +I    Q
Sbjct: 455 NRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIINNFFQ 514

Query: 424 KDVNINVPTAPEVGLYLDECLFTSYNQK-WKDSHEEVSMKAYEEVAEDFKMKQIYSHIAS 479
               IN+P AP +GL L+  +F  YN K  K  +E +    +++   +FKMK IY  I +
Sbjct: 515 -STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTKFDKEMNEFKMKYIYDKIYA 539

[Close]

BLAST of HG10013914 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.6e-49
Identity = 133/431 (30.86%), Postives = 206/431 (47.80%), Query Frame = 0


Query: 51  RYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARS 110
           R  +RK+A    YCG GY GMQ NP  KTIE D+ +A   AGA+ + +    K+  + R+
Sbjct: 81  RQPKRKVACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 140

Query: 111 ARTDKGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRY 170
           ARTDKGV A G V+S +  I+    V+++N +L  Q+ ++G  R   +F  +K C  R Y
Sbjct: 141 ARTDKGVHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVY 200

Query: 171 VYLIPVFAL---DPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTV 230
            YL+P ++     P      + +K     D E                  GK+ +E    
Sbjct: 201 EYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEE------------------GKKYWE---- 260

Query: 231 ITESDISSNTGTAIEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSE 290
                           +D++   V  D              ++ +C +   G E    +E
Sbjct: 261 -------------SVAADLESQGVSYDE------------WMKRACIDEIKGEETKEVAE 320

Query: 291 GSYMSGLGNDSEIVVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRT 350
               +    D+  +   ++V R  +    F+   E  A+   ILK Y GTHNFHNFT   
Sbjct: 321 SEVKTDSKTDAATLEKIKAVERRHRE--EFRISGERLAKIREILKIYEGTHNFHNFTLGK 380

Query: 351 KAEDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPE 410
             +DP+A R + S   S+  +++G E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+
Sbjct: 381 AFKDPSAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQ 440

Query: 411 SLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKD--SHEEVSMKAYEEVAEDFKM 470
            LI +  +K   IN+P AP +GL L+  ++ SYN+K +     E V    + +  E FK 
Sbjct: 441 KLIPQTFEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKH 461

Query: 471 KQIYSHIASTE 476
           K IY  I + E
Sbjct: 501 KFIYDKIYAEE 461

[Close]

BLAST of HG10013914 vs. ExPASy Swiss-Prot
Match: Q9Y606 (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-48
Identity = 138/459 (30.07%), Postives = 201/459 (43.79%), Query Frame = 0


Query: 40  VNSNSEAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPE 99
           + S  +  ++++  +RKI +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE
Sbjct: 69  LKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPE 128

Query: 100 QDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVT 159
                 ++  + R ARTDKGVSA GQVVS + ++     ++++N +L   I I G KRVT
Sbjct: 129 NHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVT 188

Query: 160 ASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL 219
             FN+K  CD R Y YL+P FA     H+DR                             
Sbjct: 189 GGFNSKNRCDARTYCYLLPTFAF---AHKDR----------------------------- 248

Query: 220 MGKRNFEVKTVITESDISSNTGTAIEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTEL 279
                                       D+Q+                            
Sbjct: 249 ----------------------------DVQDE--------------------------- 308

Query: 280 NVGSEGTAKSEGSYMSGLGNDSEIVVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVG 339
                                                  +++   E   + NR+L  Y G
Sbjct: 309 ---------------------------------------TYRLSAETLQQVNRLLACYKG 368

Query: 340 THNFHNFTTRTKAEDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLA 399
           THNFHNFT++   +DP+A R+I+        V EG+EF    V GQSFM+HQIRKM+GL 
Sbjct: 369 THNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLV 399

Query: 400 VAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKA 459
           VAI++  APES++E++   +  ++VP AP +GL L+   F  YNQ++ +   HE +    
Sbjct: 429 VAIVKGYAPESVLERSWGTE-KVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQ 399

Query: 460 YEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNY 494
            E     FK + IY  I  TE  + ++A WL +L   N+
Sbjct: 489 EEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNF 399

[Close]

BLAST of HG10013914 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 8.6e-48
Identity = 147/495 (29.70%), Postives = 212/495 (42.83%), Query Frame = 0


Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAY 64
           PP    + PP     +PD K +       T    + V    +    ++  +RKI +  AY
Sbjct: 29  PPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++N +L   I I G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 244
              H+DR                                                     
Sbjct: 209 --AHKDR----------------------------------------------------- 268

Query: 245 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 304
               D+Q+                                                    
Sbjct: 269 ----DVQDE--------------------------------------------------- 328

Query: 305 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 364
                          S++   E   + NR+L  Y GTHNFHNFT++    +P+A R+I+ 
Sbjct: 329 ---------------SYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILE 388

Query: 365 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 424
                  V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++
Sbjct: 389 MYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVD 395

Query: 425 VPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 484
           VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I STE  +
Sbjct: 449 VPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEEGKVTAFKEQYIYPTIVSTERDE 395

Query: 485 GAVALWLHSLNHRNY 494
            ++A WL++L   N+
Sbjct: 509 RSMAQWLNTLPIHNF 395

[Close]

BLAST of HG10013914 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 1.5e-47
Identity = 147/495 (29.70%), Postives = 212/495 (42.83%), Query Frame = 0


Query: 5   PPTETRRSPPQPLIEEPDPK-KLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAY 64
           PP    + PP     +PD K +       T    + V    +    ++  +RKI +  AY
Sbjct: 29  PPMAGNKVPPALPSHQPDRKGRGGWVWEETEHPAKRVKGGEDEEPPRKLPKRKIVLLMAY 88

Query: 65  CGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 124
            G GY GMQ+N G+   +TIE DL  AL  AG +PE      ++  + R ARTDKGVSA 
Sbjct: 89  SGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKGVSAA 148

Query: 125 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 184
           GQVVS + ++     +D++N +L   I I G KRVT  FN+K  CD R Y Y++P FA  
Sbjct: 149 GQVVSLKVWL-IDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAF- 208

Query: 185 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 244
              H+DR                                                     
Sbjct: 209 --AHKDR----------------------------------------------------- 268

Query: 245 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 304
               D+Q+                                                    
Sbjct: 269 ----DVQDE--------------------------------------------------- 328

Query: 305 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 364
                          S++   E   + NR+L  Y GTHNFHNFT++    +P+A R+I+ 
Sbjct: 329 ---------------SYRLSAETLQQVNRLLGCYKGTHNFHNFTSQKGPREPSARRYILE 388

Query: 365 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 424
                  V EG+EF   +V GQSFM+HQIRKM+GL VAI++  APES++E++  ++  ++
Sbjct: 389 MYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEE-KVD 395

Query: 425 VPTAPEVGLYLDECLFTSYNQKWKDS--HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKD 484
           VP AP +GL L+   F  YNQ++     HE +     E     FK + IY  I STE  +
Sbjct: 449 VPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEEGKVTAFKEQYIYPTIVSTERDE 395

Query: 485 GAVALWLHSLNHRNY 494
            ++A WL++L   N+
Sbjct: 509 RSMAQWLNTLPIHNF 395

[Close]

BLAST of HG10013914 vs. ExPASy TrEMBL
Match: A0A1S3BV07 (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.6e-275
Identity = 489/519 (94.22%), Postives = 498/519 (95.95%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT ITESDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TEL V SEG AKSE S MSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of HG10013914 vs. ExPASy TrEMBL
Match: A0A5A7VBT0 (tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00860 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.6e-275
Identity = 489/519 (94.22%), Postives = 498/519 (95.95%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT ITESDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TEL V SEG AKSE S MSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of HG10013914 vs. ExPASy TrEMBL
Match: E5GCD2 (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.6e-275
Identity = 489/519 (94.22%), Postives = 498/519 (95.95%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKT ITESDISSN   A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTAITESDISSNIVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TEL V SEG AKSE S MSGLGNDSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELTVASEGIAKSESSEMSGLGNDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EKPSGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASTTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKNTSV METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNTSVDMETQAET 519

[Close]

BLAST of HG10013914 vs. ExPASy TrEMBL
Match: A0A0A0L0J9 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.6e-273
Identity = 485/519 (93.45%), Postives = 497/519 (95.76%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPTETRRSPPQPLIEEP PKKLKMSSTTTTSDDE VNSNS APKKQRYKRRK+AIF
Sbjct: 1   MEEEPPTETRRSPPQPLIEEPQPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGL+GKRNFEVKT ITESDISSNT  A
Sbjct: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLIGKRNFEVKTAITESDISSNTVNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N VLED+ATTSDSNKQTSVLVEN+ TELNVGSEGTAKSE S MSGLG+DSEI
Sbjct: 241 IEVSDVQANGVLEDSATTSDSNKQTSVLVENTATELNVGSEGTAKSESSEMSGLGSDSEI 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSKESVN  EK SGSFKYGEEEKARFNRILK+YVGTHNFHNFTTRTKAEDPAA RFIVS
Sbjct: 301 VVSKESVNGKEKSSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDAS  VVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN
Sbjct: 361 FDASAIVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKD+HEEVSMKAYEE AEDFKMKQIYSHIASTEHKDGA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDTHEEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV+DEVA+IAKN S+ METQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVIDEVAKIAKNASLDMETQAET 519

[Close]

BLAST of HG10013914 vs. ExPASy TrEMBL
Match: A0A6J1IJ76 (tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.9e-268
Identity = 476/519 (91.71%), Postives = 490/519 (94.41%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGVNSNSEAPKKQRYKRRKIAIF 60
           ME+EPPT+TRRSPPQP IEEP+PKKLKMSSTTTTSDDE VNSNS APKKQRYKRRKIAIF
Sbjct: 1   MEEEPPTQTRRSPPQPSIEEPEPKKLKMSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIF 60

Query: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120
           FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV
Sbjct: 61  FAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAV 120

Query: 121 GQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180
           GQVVSGRFYIDPPGFVDRLN NLSPQI IFGYKRVTASFNAKKFCDRRRYVYLIPVFALD
Sbjct: 121 GQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALD 180

Query: 181 PNCHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTA 240
           PNCHRDRESVKASLGS NELVKCLECSERGRKVEGLMGKRNFEVKT I+ESDIS N G A
Sbjct: 181 PNCHRDRESVKASLGSGNELVKCLECSERGRKVEGLMGKRNFEVKTAISESDISPNFGNA 240

Query: 241 IEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEI 300
           IEVSD+Q N  LEDNAT SDS K+TSVLVENS TELNVGSE  AKSEGS MSGLGNDSE 
Sbjct: 241 IEVSDVQANGALEDNATASDSIKETSVLVENSGTELNVGSEAIAKSEGSEMSGLGNDSEN 300

Query: 301 VVSKESVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVS 360
           VVSK+SVNRNEKPSGSFKYGEEEKARFNRILK YVGTHNFHNFTTRTKAEDPAA RFI+S
Sbjct: 301 VVSKDSVNRNEKPSGSFKYGEEEKARFNRILKKYVGTHNFHNFTTRTKAEDPAARRFIIS 360

Query: 361 FDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNIN 420
           FDA+ TVVVEG+EFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQ+DVNIN
Sbjct: 361 FDAAITVVVEGVEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQQDVNIN 420

Query: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGA 480
           VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMK IYSHI STEHK+GA
Sbjct: 421 VPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGA 480

Query: 481 VALWLHSLNHRNYPDLRVVDEVAEIAKNTSVAMETQAET 520
           VALWLHSLNHRNYPDLRV DE A+ AKNTSV +ETQAET
Sbjct: 481 VALWLHSLNHRNYPDLRVADEAADNAKNTSVDIETQAET 519

[Close]

BLAST of HG10013914 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.5e-153
Identity = 295/508 (58.07%), Postives = 364/508 (71.65%), Query Frame = 0


Query: 1   MEKEPPTETRRSPPQPLIEEPDPKKLKMSSTTTTSDDEGV---NSNSEAPKKQRYKRRKI 60
           ME + P   +         EPD KKLK+S+T   +D   V    SN    +  +Y+RRK+
Sbjct: 1   MENQEPDIEKLPRDDVPDREPDHKKLKISTTGQETDSSAVTTGGSNVAERRAPKYRRRKV 60

Query: 61  AIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGV 120
           AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ AGAVP+ DR   + Y+WARSARTDKGV
Sbjct: 61  AIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGV 120

Query: 121 SAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVF 180
           SAVGQVVSGRFY+DPPGFV+RLN  L  QI +FGYKRV  SF++KKFCDRRRYVYLIPVF
Sbjct: 121 SAVGQVVSGRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVF 180

Query: 181 ALDPNCHRDRESVKASLGSDNELVKCLECSERGRKVE-GLMGKRN--FEVKTVITESDIS 240
           ALDP  H + E V+  LG   E VKC+ECSE+G K+  G+MGK     + K++  +SDIS
Sbjct: 181 ALDPCVHGEAEMVRTDLG--YEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDIS 240

Query: 241 SNTGTAIEVSDIQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGL 300
           S+T  AI  +D+ +   L  +  ++++N  + VL     + L+  +EG  +S        
Sbjct: 241 SSTCDAIG-ADV-KCETLSSSVPSAENNLNSEVLDGADVSALSSKAEGMEESN------- 300

Query: 301 GNDSEIVVSKESVNRNE-----KPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKA 360
                  ++K  +N  E     K    F YGE+EK RFNRIL  YVG+HNFHNFTTRTKA
Sbjct: 301 ------TLAKVEMNNGEGEDMTKSESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKA 360

Query: 361 EDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLI 420
            DPAA+R+I+SF+A+  + ++G +FVKCEVVGQSFMLHQIRKM+GLAVAIMRN APESLI
Sbjct: 361 ADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLI 420

Query: 421 EKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSHEEVSMKAYEEVAEDFKMKQIYS 480
           E + +KDV INVP APEVGLYLDEC FTSYN+++K SHEEVSM+ Y+EVAE+FK K +YS
Sbjct: 421 EASFKKDVRINVPMAPEVGLYLDECFFTSYNKRFKGSHEEVSMEEYKEVAEEFKWKYVYS 480

Query: 481 HIASTEHKDGAVALWLHSLNHRNYPDLR 498
           HI S E KDGAVA+WLHSLN RNYPDLR
Sbjct: 481 HIGSAEEKDGAVAIWLHSLNQRNYPDLR 491

[Close]

BLAST of HG10013914 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 6.8e-149
Identity = 283/470 (60.21%), Postives = 340/470 (72.34%), Query Frame = 0


Query: 31  TTTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++  ++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLN NL  QI IF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEVKTVITESDISSNTGTAIEVSDIQENRVLEDNATTSDSNKQTS 270
           RK+  GL+GK    NF  K++  +SDISSN  +A+  SDI+   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEIVVSKESVNRNEKPSGSFKYGEEEKAR 330
            + E+SC +LN  S  T                            K    F YGE+EK R
Sbjct: 243 RIQEDSC-KLNTNSSET----------------------------KVKSKFCYGEKEKER 302

Query: 331 FNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A+R I+SF A+  + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 362

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDS 450
           HQIRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYLDEC FTSYN+ ++DS
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDS 422

Query: 451 HEEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDL 497
           HEEVSM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 HEEVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

[Close]

BLAST of HG10013914 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.3e-110
Identity = 222/389 (57.07%), Postives = 270/389 (69.41%), Query Frame = 0


Query: 31  TTTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 90
           TTTT ++  ++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 91  AGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNLNLSPQILIF 150
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLN NL  QI IF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 151 GYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECSERG 210
           GYK VT SF++KKFCDRRRYVYL+PVFALDP  HRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 211 RKV-EGLMGK---RNFEVKTVITESDISSNTGTAIEVSDIQENRVLEDNATTSDSNKQTS 270
           RK+  GL+GK    NF  K++  +SDISSN  +A+  SDI+   +  + A     + +  
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALR-SDIKIEALSSNLAGLCSVDVEVG 242

Query: 271 VLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEIVVSKESVNRNEKPSGSFKYGEEEKAR 330
            + E+SC +LN  S  T                            K    F YGE+EK R
Sbjct: 243 RIQEDSC-KLNTNSSET----------------------------KVKSKFCYGEKEKER 302

Query: 331 FNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVSFDASNTVVVEGIEFVKCEVVGQSFML 390
           F+RIL  YVG++NFHNFTTRTKA+DP A+R I+SF A+  + ++GI+F+KCEV+G+SFML
Sbjct: 303 FSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFML 356

Query: 391 HQIRKMMGLAVAIMRNCAPESLIEKALQK 416
           HQIRKMMGLAVAIMRNCA ESLI+ A  K
Sbjct: 363 HQIRKMMGLAVAIMRNCASESLIQSAFSK 356

[Close]

BLAST of HG10013914 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.3e-24
Identity = 115/426 (27.00%), Postives = 178/426 (41.78%), Query Frame = 0


Query: 23  PKKLKMSST-----TTTSDDEGVNSNSEAPKKQRYKRRKIAIFFAYCGVGYQGMQ---KN 82
           P  L+ SS+     ++TS     + N  A K + Y+++K+ I   Y G  Y+G+Q    +
Sbjct: 26  PALLRFSSSAAFQPSSTSLSPPSSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDD 85

Query: 83  PGAKTIEADLEEALYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI---- 142
           P  KTIE +LE A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I    
Sbjct: 86  PSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETA 145

Query: 143 ---DPPGFV--DRLNLNLSPQILIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHR 202
              DP G V    ++ +L   I +F        F+ ++ C  R+Y YL+PV  L      
Sbjct: 146 WKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVL------ 205

Query: 203 DRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEVKTVITESDISSNTGTAIEVSD 262
               +K S  SD       + +E  ++ EG     N+  ++                   
Sbjct: 206 ---GIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRK----------SEQK 265

Query: 263 IQENRVLEDNATTSDSNKQTSVLVENSCTELNVGSEGTAKSEGSYMSGLGNDSEIVVSKE 322
           I++          S    ++    EN    + +G E   K         G   E VV+ +
Sbjct: 266 IKQRNGRPPREPKSIKASESEFREEN---HIEIGEEEEEKEVD------GESDEHVVTPD 325

Query: 323 SVNRNEKPSGSFKYGEEEKARFNRILKNYVGTHNFHNFTTRTKAEDPAAHRFIVSFDASN 382
           S N        + Y  +E  + +       G H    F  R             S    N
Sbjct: 326 SDNSQVYSRAKWLYEPDETDKIS-------GAHFRKVFRCR-------------SGKLEN 385

Query: 383 TVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAP 432
           ++   G  FV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P AP
Sbjct: 386 SL---GFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAP 400

[Close]

BLAST of HG10013914 vs. TAIR 10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein )

HSP 1 Score: 67.8 bits (164), Expect = 3.0e-11
Identity = 34/53 (64.15%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 446 SHEEVSMKAYEEVAED-FKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLR 498
           S  EVS+   +E   D FK K +YSHI S E KDGAVA+WLHSLN RNYPDLR
Sbjct: 82  SWREVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLR 134

The following BLAST results are available for this feature:

BLAST of HG10013914 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899088.1	2.8e-277	95.18	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_008452911.1	3.4e-275	94.22	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_004145528.1	5.4e-273	93.45	tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein...	[more]
XP_022975443.1	4.0e-268	91.71	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
XP_022936288.1	2.4e-265	90.96	tRNA pseudouridine synthase A [Cucurbita moschata]	[more]

BLAST of HG10013914 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q59S63	1.8e-53	32.16	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6CC39	4.6e-49	30.86	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q9Y606	1.7e-48	30.07	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]
Q9WU56	8.6e-48	29.70	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]
Q4KM92	1.5e-47	29.70	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]

BLAST of HG10013914 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BV07	1.6e-275	94.22	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7VBT0	1.6e-275	94.22	tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
E5GCD2	1.6e-275	94.22	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
A0A0A0L0J9	2.6e-273	93.45	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G65...	[more]
A0A6J1IJ76	1.9e-268	91.71	tRNA pseudouridine synthase A OS=Cucurbita maxima OX=3661 GN=LOC111474781 PE=3 S...	[more]

BLAST of HG10013914 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20370.1	3.5e-153	58.07	Pseudouridine synthase family protein	[more]
AT1G76120.1	6.8e-149	60.21	Pseudouridine synthase family protein	[more]
AT1G76120.2	1.3e-110	57.07	Pseudouridine synthase family protein	[more]
AT2G30320.1	2.3e-24	27.00	Pseudouridine synthase family protein	[more]
AT1G20290.1	3.0e-11	64.15	SWI-SNF-related chromatin binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 331..436 e-value: 7.4E-14 score: 52.1
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 298..500 e-value: 5.5E-55 score: 188.0
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 46..165 e-value: 8.5E-27 score: 95.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..41
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR11142:SF24	TRNA PSEUDOURIDINE SYNTHASE	coord: 31..497
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 31..497
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 59..436 e-value: 1.02596E-102 score: 306.462
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..446

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10013914.1	HG10013914.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding