HG10013901 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013901
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionButanoate--CoA ligase AAE1
LocationChr02: 5856257 .. 5858288 (+)
RNA-Seq ExpressionHG10013901
SyntenyHG10013901
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGGAATCGACCGTTGCTCTGCCAATTACGTTCCTCTCACTCCGATCACCTTCTTGGAGCGCTCCGCCGCGGTCTATGGTGCTCGGATTTCTCTAGTCTATGGACGTGTTCAGTACACTTGGAGAGACACGCTTGAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTATCGGAATCGCTCGTGGAGATGTGGTCTGTATTCACATCTACTCTCACTTTCATTCTTACTCTCTGGATATTTGTTTATGTTTTTTCTGGTTTTACTCGATTGTTTTGAGGTCTTTGTGTTTATGTAGAACAAGAAATTATTTGATCTGCAAGTTACTTCGATGCCATTGTTTTATGATTTTGATTATTACTTGCGATGATTTGAAGCTCACGGATCTGATTTTGTGTTTACGGTGATAGTTATTCAGATGGATGAAATCCGGTTCCCTTCTTATTATGGTTAACTGTGAAATTATAGTTTGAAAGTGGCATTTGGTTAGATTAAGGTCTTTCTTCGTTACATTTTCTCGACATAATTTTGAAAATGAAATACGATTCTCTCGAACAGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGCTACATTTTGCTGTACCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACATACGCCATGATGCAGCAATGGTTTCTACATTGCTAAGTCATTCGGAAGCTAAAATCATTTTTGTAGACTATCAGTTTGAACATATTGTAATTGGAGCAATTAAAGCTATGTCCGAGAGGAGAGAGAACCTACCTCGTGTTGTTATTATTCAAGAGTATGATCAGCCACCTTCCCACATCGATAGACCAGGCTCTGCTTTAGAATATCTAGAGTTCGAGAGTTTTTTAGCATCTGGAAAACTCGATTTCGAGATCAGGCGACCTAAGGATGAATGGGATCCAATTGCTCTTAACTATACTTCAGGCACAACATCAAGGCCAAAGGGTGTTATTTTCTCCCATAGAGGTGCATATCTCAATGCTCTGTCTGCAGTTCTACTGAATGATATGTGCTCACTCCCTGTGTATTTGTGGACTGTTCCAATGTTTCATTGCAATGGATGGTGTTTAACTTGGGGTGTAGCTGCACAGAGTGGCACAAACATCTGCCAGAGAAATGTGACTGCTAAAGAAATCTTTGATAATATTTCTCTGCATAAGGTTACTCATATGGGTGGTGCACCAACAGTTTCGAACATGATTATCAATGCACCAATTAGTGAACAGAAGCCACTTCCCAGGCAGGTAACTATGATGTCTGGTGGCGCTCCACCGCCTTCTCATGTACTCTATAAGCTTAGAGCTATGGGATTTCGTATTGTTCATTCATATGGTTTAACTGAAACATATGGGCCAGCAACAGTTTGCTCTTGGAAGCCTGAATGGGATTCACTCCCTCAAGATGAACAAGCAAAACTAAGTTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGTAGATGTAAAGGATCCTGTCACAATGGAGAGTACTCCAGCTGATGGAAAAACCATGGGTGAAGTTATGCTCAGAGGCAACACTGTGATGAGTGGATATCTGAAAGATCTCAAAGCCACACGAGAAGCTTTTAATGGTGGATGGTTTCGAAGTGGGGACTTGGGGGTCAAACATCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATTATCATTTCTGGGGGAGAAAATATTAGCTCCATTGAGGTTGAGTCTGTTCTTTTCGGTCATCCATCAGTTCTTGAAGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGTGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGAAGAGATTATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTATGGATTTACCAAAAACTTCTACAGGGAAAACCCAAAAATTTATTCTCAAGAAGGAAGCCAAGGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAA

mRNA sequence

ATGGACGGAATCGACCGTTGCTCTGCCAATTACGTTCCTCTCACTCCGATCACCTTCTTGGAGCGCTCCGCCGCGGTCTATGGTGCTCGGATTTCTCTAGTCTATGGACGTGTTCAGTACACTTGGAGAGACACGCTTGAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTATCGGAATCGCTCGTGGAGATGTGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGCTACATTTTGCTGTACCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACATACGCCATGATGCAGCAATGCCTGAATGGGATTCACTCCCTCAAGATGAACAAGCAAAACTAAGTTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGTAGATGTAAAGGATCCTGTCACAATGGAGAGTACTCCAGCTGATGGAAAAACCATGGGTGAAGTTATGCTCAGAGGCAACACTGTGATGAGTGGATATCTGAAAGATCTCAAAGCCACACGAGAAGCTTTTAATGGTGGATGGTTTCGAAGTGGGGACTTGGGGGTCAAACATCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATTATCATTTCTGGGGGAGAAAATATTAGCTCCATTGAGGTTGAGTCTGTTCTTTTCGGTCATCCATCAGTTCTTGAAGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGTGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGAAGAGATTATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTATGGATTTACCAAAAACTTCTACAGGGAAAACCCAAAAATTTATTCTCAAGAAGGAAGCCAAGGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAA

Coding sequence (CDS)

ATGGACGGAATCGACCGTTGCTCTGCCAATTACGTTCCTCTCACTCCGATCACCTTCTTGGAGCGCTCCGCCGCGGTCTATGGTGCTCGGATTTCTCTAGTCTATGGACGTGTTCAGTACACTTGGAGAGACACGCTTGAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTATCGGAATCGCTCGTGGAGATGTGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGCTACATTTTGCTGTACCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACATACGCCATGATGCAGCAATGCCTGAATGGGATTCACTCCCTCAAGATGAACAAGCAAAACTAAGTTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGTAGATGTAAAGGATCCTGTCACAATGGAGAGTACTCCAGCTGATGGAAAAACCATGGGTGAAGTTATGCTCAGAGGCAACACTGTGATGAGTGGATATCTGAAAGATCTCAAAGCCACACGAGAAGCTTTTAATGGTGGATGGTTTCGAAGTGGGGACTTGGGGGTCAAACATCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATTATCATTTCTGGGGGAGAAAATATTAGCTCCATTGAGGTTGAGTCTGTTCTTTTCGGTCATCCATCAGTTCTTGAAGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGTGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGAAGAGATTATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTATGGATTTACCAAAAACTTCTACAGGGAAAACCCAAAAATTTATTCTCAAGAAGGAAGCCAAGGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAA

Protein sequence

MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIGIARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAMPEWDSLPQDEQAKLSSRQGLQHIGLEAVDVKDPVTMESTPADGKTMGEVMLRGNTVMSGYLKDLKATREAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESVLFGHPSVLEAAVVGRPDDHWGETPCAFVKLKDGCSATEEEIIKFCREHLPHYMAPRSVVFMDLPKTSTGKTQKFILKKEAKAMGSLPKRVSKL
Homology
[Close]
BLAST of HG10013901 vs. NCBI nr
Match: XP_008452947.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 502.3 bits (1292), Expect = 3.0e-138
Identity = 293/552 (53.08%), Postives = 302/552 (54.71%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDGI RCSANYVPLTPITFLERSAAVYG RISLVYGRVQYTWRDTLERCTRLASALVR G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM                  
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LEHIVTGAINTMSERKEKLPRVVIIQEYDQPPSHIDRPGSALEFESFLASGELDFEIRRP 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMFHCNGW 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRQ 300

Query: 301 --------------------------------------------PEWDSLPQDEQAKLSS 311
                                                       PEWDSLPQD+QAKL+S
Sbjct: 301 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDKQAKLNS 360

[Close]
BLAST of HG10013901 vs. NCBI nr
Match: XP_038898242.1 (probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898244.1 probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898245.1 probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898246.1 probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898247.1 probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898248.1 probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida])

HSP 1 Score: 502.3 bits (1292), Expect = 3.0e-138
Identity = 295/555 (53.15%), Postives = 298/555 (53.69%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDGIDRC+ANYVPLTPITFLERSAAVYG RISLVYGRVQYTWRDTL RCT+LASA VR G
Sbjct: 1   MDGIDRCAANYVPLTPITFLERSAAVYGNRISLVYGRVQYTWRDTLRRCTKLASAFVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAV CSLNIRHDAAM                  
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVFCSLNIRHDAAMVSTLLSHSEARIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FEHIVIGAIKAMSQRKEKPPRVVIIQEYDQPPSHIDTTGSALEYLEFESFLASGRPDFEI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPKDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNIIINAPISEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQDEQAK
Sbjct: 301 PRQVTMMSGGAPLPSHVLYKLRAMGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDEQAK 360

[Close]
BLAST of HG10013901 vs. NCBI nr
Match: XP_004145536.1 (probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN55493.1 hypothetical protein Csa_012074 [Cucumis sativus])

HSP 1 Score: 501.1 bits (1289), Expect = 6.7e-138
Identity = 294/555 (52.97%), Postives = 300/555 (54.05%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDG  RCSANYVPLTPI+FLERSAAVYG RISLVYGRVQYTWRDTL+RCTRLASALVR G
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM                  
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQD+QAK
Sbjct: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

[Close]
BLAST of HG10013901 vs. NCBI nr
Match: TYK19959.1 (putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 498.8 bits (1283), Expect = 3.3e-137
Identity = 294/558 (52.69%), Postives = 303/558 (54.30%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDGI RCSANYVPLTPITFLERSAAVYG RISLVYGRVQYTWRDTLERCTRLASALVR G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDV------VAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------ 120
           IARGDV      VAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM            
Sbjct: 61  IARGDVCDSFEQVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 IVVDYQLEHIVTGAINTMSERKEKLPRVVIIQEYDQPPSHIDRPGSALEFESFLASGELD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEIRRPRDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQ 300

Query: 301 --------------------------------------------------PEWDSLPQDE 311
                                                             PEWDSLPQD+
Sbjct: 301 KPLPRQVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDK 360

[Close]
BLAST of HG10013901 vs. NCBI nr
Match: KAG7024112.1 (putative acyl-activating enzyme 1, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 496.5 bits (1277), Expect = 1.7e-136
Identity = 287/555 (51.71%), Postives = 302/555 (54.41%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDG+DRCSANY+PLTPITFLERSAAVYG RISLVYGRVQYTWR+TL RCTRLASALVR+G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAAL PNIPAMYELHFAVPMAGAVLCSLN+RHDAAM                  
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FEHIVTGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFESLLESGKLDFKI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQD+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

[Close]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.1e-102
Identity = 219/549 (39.89%), Postives = 263/549 (47.91%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           M+G  +  ANYVPLTPI+FL+RSA VY  R+S+VYG V+YTWR T +RC R+ASAL ++G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           I+ GDVV+ L PN+PAM ELHF VPMAGA+LC+LNIRHD+++                  
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 ---------------------------------------------PEWDSLPQDEQAKLS 307
                                                        PEWDSLP++EQAK+ 
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

[Close]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-91
Identity = 207/556 (37.23%), Postives = 250/556 (44.96%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           ++G+ R  AN+ PL+PITFLERSA VY  R SLV+G V++TW  T +RC RLASAL  +G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAA------------------- 120
           I+RGDVVAAL PN+PAM+ELHFAVPMAG +LC LN R D +                   
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 -----------------------------------------------------MPEWDSL 304
                                                                 PEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

[Close]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.2e-90
Identity = 205/544 (37.68%), Postives = 249/544 (45.77%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASAL-VRI 60
           M+G+ +CSAN+VPL+PITFLERS+  Y    SLVYG V+YTW  T  RC +LASAL   +
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  GIARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM----------------- 120
           GI+ GDVVA    N+P +YELHFAVPMAG +LC+LN R+D+AM                 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPMFHCNGWCFPW 268

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 269 GAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEV 328

Query: 301 -----------------------------------------PEWDSLPQDEQAKLSSRQG 304
                                                    PEWD+L  +E+  L +RQG
Sbjct: 329 MTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQG 388

[Close]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 5.4e-90
Identity = 204/544 (37.50%), Postives = 249/544 (45.77%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASAL-VRI 60
           M+G+ +CSAN+VPL+PITFLERS+  Y    SLVYG V+YTW  T  RC +LASAL    
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  GIARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM----------------- 120
           GI+ GDVVA    NIP +YELHFAVPMAG +LC+LN R+D+AM                 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPMFHCNGWCFPW 268

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 269 GAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKVEV 328

Query: 301 -----------------------------------------PEWDSLPQDEQAKLSSRQG 304
                                                    PEWD+L  +E+  L +RQG
Sbjct: 329 MTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKARQG 388

[Close]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match: Q9SFW5 (Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.2e-75
Identity = 181/543 (33.33%), Postives = 231/543 (42.54%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           M+G  RCSANYVPL+PI+FLER+A V+G+R S+VYG +QYTW  T +RC RLASAL  +G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAA------------------- 120
           ++R DVVAAL PN+PA+ EL+F  PMAGAVLC LN   D+                    
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FLSVAEESLSLLSNIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDECDPI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 ALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYHCSGWCYIWTVT 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWTVQVMSGG 300

Query: 301 -----------------------------------MPEWDSLPQDEQAKLSSRQGLQHIG 307
                                              MPEW++LP++E  +L +R GL H  
Sbjct: 301 SSPPEVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLKARDGLNHFA 360

[Close]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match: A0A1S3BUH2 (probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103493805 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.5e-138
Identity = 293/552 (53.08%), Postives = 302/552 (54.71%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDGI RCSANYVPLTPITFLERSAAVYG RISLVYGRVQYTWRDTLERCTRLASALVR G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM                  
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LEHIVTGAINTMSERKEKLPRVVIIQEYDQPPSHIDRPGSALEFESFLASGELDFEIRRP 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMFHCNGW 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRQ 300

Query: 301 --------------------------------------------PEWDSLPQDEQAKLSS 311
                                                       PEWDSLPQD+QAKL+S
Sbjct: 301 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDKQAKLNS 360

[Close]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match: A0A0A0L1B6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658530 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.3e-138
Identity = 294/555 (52.97%), Postives = 300/555 (54.05%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDG  RCSANYVPLTPI+FLERSAAVYG RISLVYGRVQYTWRDTL+RCTRLASALVR G
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM                  
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQD+QAK
Sbjct: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

[Close]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match: A0A5D3D8Z9 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00720 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.6e-137
Identity = 294/558 (52.69%), Postives = 303/558 (54.30%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDGI RCSANYVPLTPITFLERSAAVYG RISLVYGRVQYTWRDTLERCTRLASALVR G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDV------VAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------ 120
           IARGDV      VAALVPNIPAMYELHFAVPMAGAVLCSLN RHDAAM            
Sbjct: 61  IARGDVCDSFEQVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKI 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 IVVDYQLEHIVTGAINTMSERKEKLPRVVIIQEYDQPPSHIDRPGSALEFESFLASGELD 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEIRRPRDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPM 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 FHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQ 300

Query: 301 --------------------------------------------------PEWDSLPQDE 311
                                                             PEWDSLPQD+
Sbjct: 301 KPLPRQVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDK 360

[Close]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match: A0A6J1FFJ8 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111443496 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 8.0e-137
Identity = 287/555 (51.71%), Postives = 302/555 (54.41%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDG+DRCSANY+PLTPITFLERSAAVYG RISLVYGRVQYTWR+TL RCTRLASALVR+G
Sbjct: 1   MDGMDRCSANYLPLTPITFLERSAAVYGDRISLVYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAAL PNIPAMYELHFAVPMAGAVLCSLN+RHDAAM                  
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FEHIVMGAIKAMSERREKIPLVVIINEYDQPPSHIDREGSASGYLEFESLLESGKLDFKI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPKDELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNASAKEIFDNISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQD+QAK
Sbjct: 301 PGKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

[Close]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match: A0A6J1IF13 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111473618 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.0e-135
Identity = 285/555 (51.35%), Postives = 300/555 (54.05%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           MDG+DRCSANYVPLTPITFLERSAAVYG RISL YGRVQYTWR+TL RCTRLASALVR+G
Sbjct: 1   MDGMDRCSANYVPLTPITFLERSAAVYGDRISLAYGRVQYTWRETLLRCTRLASALVRMG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           IARGDVVAAL PNIPAMYELHFAVPMAGAVLCSLN+RHDAAM                  
Sbjct: 61  IARGDVVAALAPNIPAMYELHFAVPMAGAVLCSLNVRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FEHIVMGAIKAMSERREKIPLVVIINEYDQPPSYIDREGSASEYLEFESLLESGKLDFKI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 RRPKYELDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWVVPMFHC 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 NGWSLTWGVAAQSGTNICQRNVSAKEIFENISLHKVTHMGGAPTVLNMIINAPTSEQKPL 300

Query: 301 -----------------------------------------------PEWDSLPQDEQAK 311
                                                          PEWDSLPQD+QAK
Sbjct: 301 PRKVTMMTGGAPPPSHVLFKMRAMGFLIVHAYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

[Close]
BLAST of HG10013901 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 373.6 bits (958), Expect = 1.5e-103
Identity = 219/549 (39.89%), Postives = 263/549 (47.91%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           M+G  +  ANYVPLTPI+FL+RSA VY  R+S+VYG V+YTWR T +RC R+ASAL ++G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAAM------------------ 120
           I+ GDVV+ L PN+PAM ELHF VPMAGA+LC+LNIRHD+++                  
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 ---------------------------------------------PEWDSLPQDEQAKLS 307
                                                        PEWDSLP++EQAK+ 
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

[Close]
BLAST of HG10013901 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.2e-92
Identity = 207/556 (37.23%), Postives = 250/556 (44.96%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           ++G+ R  AN+ PL+PITFLERSA VY  R SLV+G V++TW  T +RC RLASAL  +G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAA------------------- 120
           I+RGDVVAAL PN+PAM+ELHFAVPMAG +LC LN R D +                   
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 -----------------------------------------------------MPEWDSL 304
                                                                 PEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

[Close]
BLAST of HG10013901 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 327.8 bits (839), Expect = 9.5e-90
Identity = 153/204 (75.00%), Postives = 178/204 (87.25%), Query Frame = 0 

Query: 103 PEWDSLPQDEQAKLSSRQGLQHIGLEAVDVKDPVTMESTPADGKTMGEVMLRGNTVMSGY 162
           PEWDSLP++EQAK+ +RQG+ H+GLE + VKDPVTM + PADG TMGEV+ RGNTVM+GY
Sbjct: 270 PEWDSLPREEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGY 329

Query: 163 LKDLKATREAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESVLFGHP 222
           LK+ +AT+EAF GGWF SGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVES LF HP
Sbjct: 330 LKNPEATKEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHP 389

Query: 223 SVLEAAVVGRPDDHWGETPCAFVKLKDGCSATEEEIIKFCREHLPHYMAPRSVVFMDLPK 282
            VLEAAVV RPD++WGET CAFVKLKDG  A+ EE+I +CR+ LPHYMAPRS+VF DLPK
Sbjct: 390 CVLEAAVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPK 449

Query: 283 TSTGKTQKFILKKEAKAMGSLPKR 307
           TSTGK QKF+L+ +AKA+ SL K+
Sbjct: 450 TSTGKVQKFVLRTKAKALVSLSKK 473


HSP 2 Score: 46.6 bits (109), Expect = 4.2e-05
Identity = 19/26 (73.08%), Postives = 24/26 (92.31%), Query Frame = 0 

Query: 77  MYELHFAVPMAGAVLCSLNIRHDAAM 103
           M ELHF VPMAGA+LC+LNIRHD+++
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSL 26

[Close]
BLAST of HG10013901 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.6e-76
Identity = 181/543 (33.33%), Postives = 231/543 (42.54%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           M+G  RCSANYVPL+PI+FLER+A V+G+R S+VYG +QYTW  T +RC RLASAL  +G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAA------------------- 120
           ++R DVVAAL PN+PA+ EL+F  PMAGAVLC LN   D+                    
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FLSVAEESLSLLSNIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDECDPI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 ALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYHCSGWCYIWTVT 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWTVQVMSGG 300

Query: 301 -----------------------------------MPEWDSLPQDEQAKLSSRQGLQHIG 307
                                              MPEW++LP++E  +L +R GL H  
Sbjct: 301 SSPPEVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLKARDGLNHFA 360

[Close]
BLAST of HG10013901 vs. TAIR 10
Match: AT3G16910.1 (acyl-activating enzyme 7 )

HSP 1 Score: 270.8 bits (691), Expect = 1.4e-72
Identity = 178/558 (31.90%), Postives = 228/558 (40.86%), Query Frame = 0 

Query: 1   MDGIDRCSANYVPLTPITFLERSAAVYGARISLVYGRVQYTWRDTLERCTRLASALVRIG 60
           +D + +  ANY  LTP+ FL+R+A V+  R S+++G  +YTWR T +RC RLASAL    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNIRHDAA------------------- 120
           I  G  VA + PNIPAMYE HF VPM GAVL  +NIR +A                    
Sbjct: 69  IGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQE 128

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 129 FFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNY 188

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 189 PWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMF 248

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 249 HCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTI 308

Query: 301 -------------------------------------------------MPEWDSLPQDE 306
                                                             PEWDSLP + 
Sbjct: 309 LPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPET 368

The following BLAST results are available for this feature:
BLAST of HG10013901 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008452947.13.0e-13853.08PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo][more]
XP_038898242.13.0e-13853.15probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] >XP_038898244... [more]
XP_004145536.16.7e-13852.97probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN55493.1 hyp... [more]
TYK19959.13.3e-13752.69putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa][more]
KAG7024112.11.7e-13651.71putative acyl-activating enzyme 1, peroxisomal [Cucurbita argyrosperma subsp. ar... [more]
BLAST of HG10013901 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4HUK62.1e-10239.89Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1[more]
Q9SEY51.7e-9137.23Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1[more]
M4IRL43.2e-9037.68Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1[more]
M4IS925.4e-9037.50Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1[more]
Q9SFW52.2e-7533.33Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3... [more]
BLAST of HG10013901 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BUH21.5e-13853.08probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0L1B63.3e-13852.97Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658530 PE=4 SV=1[more]
A0A5D3D8Z91.6e-13752.69Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1FFJ88.0e-13751.71probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IF132.0e-13551.35probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... [more]
BLAST of HG10013901 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G20560.11.5e-10339.89acyl activating enzyme 1 [more]
AT2G17650.11.2e-9237.23AMP-dependent synthetase and ligase family protein [more]
AT1G20560.29.5e-9075.00acyl activating enzyme 1 [more]
AT1G76290.11.6e-7633.33AMP-dependent synthetase and ligase family protein [more]
AT3G16910.11.4e-7231.90acyl-activating enzyme 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.30.38.10Luciferase; Domain 3coord: 124..197
e-value: 8.8E-16
score: 59.5
NoneNo IPR availableGENE3D3.30.300.30coord: 199..301
e-value: 5.5E-31
score: 109.2
NoneNo IPR availableGENE3D3.40.50.980coord: 15..119
e-value: 1.3E-21
score: 78.8
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 5..102
NoneNo IPR availablePANTHERPTHR43859:SF5ACYL-ACTIVATING ENZYME 2-RELATEDcoord: 5..102
NoneNo IPR availablePANTHERPTHR43859:SF5ACYL-ACTIVATING ENZYME 2-RELATEDcoord: 102..303
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 102..303
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 10..100
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 130..298
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 213..287
e-value: 3.0E-24
score: 85.8
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 20..98
e-value: 5.2E-15
score: 55.0
coord: 111..204
e-value: 1.1E-21
score: 77.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013901.1HG10013901.1mRNA