HG10013705 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10013705 vs. NCBI nr
Match: XP_038899804.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Benincasa hispida])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 555/624 (88.94%), Postives = 577/624 (92.47%), Query Frame = 0

Query: 2   ASSSSLSSL--FLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFE 61
           +SSSSLSSL   LISL FLLLPFHSNANLHK RLR SDLLSDS LTTTAD+TTPPTVFFE
Sbjct: 6   SSSSSLSSLLVLLISLIFLLLPFHSNANLHKIRLRHSDLLSDSGLTTTADYTTPPTVFFE 65

Query: 62  VTKPIETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGR 121
           VTKPIETPKAKPCSLLVLQHDF +TYGKSPVLADY+PPSHCSF+KFSKIVLEW ATC+GR
Sbjct: 66  VTKPIETPKAKPCSLLVLQHDFAFTYGKSPVLADYTPPSHCSFTKFSKIVLEWTATCQGR 125

Query: 122 QFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKT 181
           QFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLL KNQTLAVYLGNL+DKT
Sbjct: 126 QFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYDSLLSKNQTLAVYLGNLVDKT 185

Query: 182 YTGIYHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEV 241
           YTGIYHV+IH+HFYPE     D GV S+KL S Y SDSRADLILPISRNLPLNDGLWFEV
Sbjct: 186 YTGIYHVKIHVHFYPE----ADTGVSSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEV 245

Query: 242 QNSTDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFRE 301
           QNSTDVQ KEF+IPQNVYRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FRE
Sbjct: 246 QNSTDVQSKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFRE 305

Query: 302 VLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVH 361
           VLV LDGEIVGAVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLGHLLDGKVH
Sbjct: 306 VLVSLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGHLLDGKVH 365

Query: 362 NFGFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTK 421
           N GF VTHALNVWYINANLHLWLDDNSAKTEGKLL+HIVS PFIS DLNFTGP+GTFLTK
Sbjct: 366 NCGFSVTHALNVWYINANLHLWLDDNSAKTEGKLLDHIVSPPFISVDLNFTGPDGTFLTK 425

Query: 422 VNRSLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESS 481
           V RS+SSTGWVKSSFGII TQSNQDLSYSNSMVMGNNGSSQTV QEIQFN SVYAKQESS
Sbjct: 426 VTRSVSSTGWVKSSFGIINTQSNQDLSYSNSMVMGNNGSSQTVTQEIQFNGSVYAKQESS 485

Query: 482 HVYSFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQG 541
           HVYSFKSLKTFPLYMYS+T+DEGNGTYQSIANLTLGFNEKKTDG GSF SSLKNLQNGQG
Sbjct: 486 HVYSFKSLKTFPLYMYSNTIDEGNGTYQSIANLTLGFNEKKTDGFGSFTSSLKNLQNGQG 545

Query: 542 IMVVKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLR 601
           +MVVKG+LVT GLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVK+ CG +   L  L 
Sbjct: 546 LMVVKGHLVTSGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKDFCGRKTRILRKLG 605

Query: 602 FRRGPIPARRAFLDSNLHDNNGHV 624
           FR G IPARRAFLDSNLH NN HV
Sbjct: 606 FRGGSIPARRAFLDSNLHVNNSHV 625

BLAST of HG10013705 vs. NCBI nr
Match: XP_004146092.2 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis sativus] >KGN55069.1 hypothetical protein Csa_011983 [Cucumis sativus])

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 539/621 (86.80%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 3   SSSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTK 62
           +SS L  + L+ L  LLLPFHSNANLHKTRLR+SDL S+ D+TTTADH+TPPTVFFEV+K
Sbjct: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61

Query: 63  PIETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 122
           PI TPK+KPCSLL+L HDF +TYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFD
Sbjct: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121

Query: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTG 182
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLL KNQTLAVYLGNL+DKTYTG
Sbjct: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181

Query: 183 IYHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNS 242
           IY+V+I IHFYPEE+GFG NGVYS+KL S Y SDSRADLILPISRNLPLNDGLWFEVQNS
Sbjct: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 241

Query: 243 TDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLV 302
           TDV+ KEF+IPQNVYRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLV
Sbjct: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 303 DLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFG 362
           DLDGEIVGAVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGKVHNFG
Sbjct: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361

Query: 363 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNR 422
           FRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVSSP +S+DLNFTGP+GTFLTKV R
Sbjct: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421

Query: 423 SLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVY 482
           S+SSTGWVKSSFGIITT SNQDLSYSNSM MGNNGSSQTVNQEIQFN+SVYAK+ESSHVY
Sbjct: 422 SVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481

Query: 483 SFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMV 542
           S KSLKTFPLYMYS+T DE NG+YQSIANLTLGFNEKKTDGLG  ISSLKN+QNGQG++V
Sbjct: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541

Query: 543 VKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRR 602
           VKG+LVT GLGSTQQDYRYHDD+QCYSRNISSSNYTIL+DNVKN CG +  S ESLRFRR
Sbjct: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601

Query: 603 GPIPARRAFLDSNLHDNNGHV 624
            PIPARRAFLDSNL  NNG V
Sbjct: 602 WPIPARRAFLDSNLFVNNGRV 622

BLAST of HG10013705 vs. NCBI nr
Match: XP_008463736.1 (PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo] >KAA0066768.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa] >TYK27915.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 539/619 (87.08%), Postives = 579/619 (93.54%), Query Frame = 0

Query: 5   SSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKPI 64
           +S S L LISL  LLLPFHS+A+LHKTRLRQSDL S+S ++TTADH+TPPTVFFEVTKPI
Sbjct: 2   ASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPI 61

Query: 65  ETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDRI 124
            TPKAKPCSLLVL HDF YTYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFDRI
Sbjct: 62  VTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRI 121

Query: 125 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGIY 184
           FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLL KNQTLAVYLGNL+DKTYTGIY
Sbjct: 122 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGIY 181

Query: 185 HVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNSTD 244
           +V+IHIHFYPEE+GFG NGVYS+KL S Y SDSRADLILPISRNLPLNDGLWFEVQNSTD
Sbjct: 182 NVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTD 241

Query: 245 VQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLVDL 304
           VQLKEF+IPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLVDL
Sbjct: 242 VQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDL 301

Query: 305 DGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFGFR 364
           DG+IV AVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGFR
Sbjct: 302 DGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFR 361

Query: 365 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNRSL 424
           VTHALNVW+INANLHLWLDDNS KTE KLLNHIVSSP +S+DLNFTG +GTFLTKV RS+
Sbjct: 362 VTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSV 421

Query: 425 SSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVYSF 484
           SS GWVKSSFG ITT SNQDLSYSN M+MGNNGSSQTVNQEI FNSSVYAKQESSHVYS 
Sbjct: 422 SSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSS 481

Query: 485 KSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 544
           K+LKTFPLYMYS+T+D+GNG+Y+SIANLTLGFNEKKTDGLGSFISSLKN+QNGQG+MVVK
Sbjct: 482 KTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVK 541

Query: 545 GNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRRGP 604
           GNLVT GLGSTQQDYRY+DD+QCYSR+ISSSNYTIL+DNVKN+CG +  S ESLRFRR P
Sbjct: 542 GNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRWP 601

Query: 605 IPARRAFLDSNLHDNNGHV 624
           IPARRA LDSNL  NNGHV
Sbjct: 602 IPARRASLDSNLFVNNGHV 620

BLAST of HG10013705 vs. NCBI nr
Match: XP_022140996.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Momordica charantia])

HSP 1 Score: 1060.8 bits (2742), Expect = 4.4e-306
Identity = 524/621 (84.38%), Postives = 562/621 (90.50%), Query Frame = 0

Query: 4   SSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKP 63
           +SSLSS   ISL  LLLPF+S+ANLHKTR R+ DLLS+   T    HT PPT+FFEVTKP
Sbjct: 2   ASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTKP 61

Query: 64  IETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 123
           IETPKAKPCS+L LQHDFGYTYGKSPVLADY+PPSHCSF+KFSKIVLEW ATCRGRQFDR
Sbjct: 62  IETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFDR 121

Query: 124 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGI 183
           IFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY+SLL KNQTLAVYLGNL+DKTYTGI
Sbjct: 122 IFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 181

Query: 184 YHVEIHIHFYPEEKGFGDN-GVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNS 243
           YHV IHIHFYPEEK FGDN G + +K ASGY  DS ADLI+PISR+LPLNDGLWFEVQ+S
Sbjct: 182 YHVRIHIHFYPEEKSFGDNGGRFLEKSASGY--DSGADLIVPISRSLPLNDGLWFEVQSS 241

Query: 244 TDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLV 303
           TDVQ+KEF+IPQNVYRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLV
Sbjct: 242 TDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 304 DLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFG 363
            LDG+IVGAVWPFTV+YTGGVNPLLWRPITGIGSFNLPSYDIE+TPFLG+LLDGK HNFG
Sbjct: 302 SLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFG 361

Query: 364 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNR 423
           FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVS    SE+ NFTGP+GTF TKV R
Sbjct: 362 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTR 421

Query: 424 SLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVY 483
           S+SS GWVKSS GIITTQSNQD SYSNSM +G+NGSSQ VNQ+IQFNSSVYAKQ+SSHVY
Sbjct: 422 SVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVY 481

Query: 484 SFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMV 543
           SFKSLKTFPLY+YSDT DEGNGTYQS+ANLTLGFNEKKTDG GSFISSLKNLQNGQG+MV
Sbjct: 482 SFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMV 541

Query: 544 VKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRR 603
           VK +LVTRGLGSTQQDYRYHDD  CY RNISSSNYTIL+DNVKN CG + ES  SLRF R
Sbjct: 542 VKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGR 601

Query: 604 GPIPARRAFLDSNLHDNNGHV 624
            PIPARRAFLDS+L  N+ HV
Sbjct: 602 WPIPARRAFLDSSLQVNSVHV 620

BLAST of HG10013705 vs. NCBI nr
Match: XP_022941014.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A-like [Cucurbita moschata])

HSP 1 Score: 1047.0 bits (2706), Expect = 6.6e-302
Identity = 512/609 (84.07%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 4   SSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKP 63
           +SSLSS  LISL F+LLPFHSNA+LH+T LR+ DL S+S+L  T +HTTPPT+FFEVTKP
Sbjct: 2   ASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTKP 61

Query: 64  IETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 123
           IETP+A+PCSLL+LQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR
Sbjct: 62  IETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 121

Query: 124 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGI 183
           IFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLL KNQTLAVYLGNLIDKTYTGI
Sbjct: 122 IFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGI 181

Query: 184 YHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNST 243
           YHV+IHIHFYPEEK F     YS+KLA+GY  DSRADLILPISR+LPLNDGLWFEVQNST
Sbjct: 182 YHVKIHIHFYPEEKRF----QYSEKLAAGY--DSRADLILPISRSLPLNDGLWFEVQNST 241

Query: 244 DVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLVD 303
           D+Q+KEF+IPQN YRAVLEVY+SFHENDEFWYSNLPNDYI ANNL+ TPGNG FREVLV+
Sbjct: 242 DIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLVN 301

Query: 304 LDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFGF 363
           LD EIVGA+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+FGF
Sbjct: 302 LDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGF 361

Query: 364 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNRS 423
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI+S    SEDLNFTGP+GTFLTK +RS
Sbjct: 362 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRS 421

Query: 424 LSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVYS 483
           +SS+GWVKSS GIITTQSNQD SYSNSMVMGN+G+SQ VNQEIQFNSSVYAK+ES HVYS
Sbjct: 422 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVYS 481

Query: 484 FKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 543
           FKSLKTFPLYMYS+T+DEGNG++QSIANLTLGFNEKK DGLGSFISSLKN+QNGQG+M V
Sbjct: 482 FKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTV 541

Query: 544 KGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRRG 603
           KG+LVTRGLGSTQQDYRYHDD  CYSRNISSSNYTILYD+V ++C  + ES ESLRFR+ 
Sbjct: 542 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKW 601

Query: 604 PIPARRAFL 613
           P  +RR FL
Sbjct: 602 PFLSRRVFL 604

BLAST of HG10013705 vs. ExPASy Swiss-Prot
Match: P81898 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Prunus dulcis OX=3755 PE=1 SV=2)

HSP 1 Score: 776.9 bits (2005), Expect = 1.7e-223
Identity = 391/563 (69.45%), Postives = 442/563 (78.51%), Query Frame = 0

Query: 50  HTTPPTVFFEVTKPIETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIV 109
           H TPPTVFFEVTKPIE PK KPCS L+LQHDF YTYG++PV A+Y+PPS C    FS IV
Sbjct: 6   HDTPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIV 65

Query: 110 LEWKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLA 169
           LEWKATCR RQFDRIFGVWLGGVEILRSCTAEPR  GIVWTV+KDITRY SLL  NQTLA
Sbjct: 66  LEWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLA 125

Query: 170 VYLGNLIDKTYTGIYHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNL 229
           VYLGNLIDKTYTGIYHV I +HFYP ++           LASGY   S ADLILPISRNL
Sbjct: 126 VYLGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGY--HSWADLILPISRNL 185

Query: 230 PLNDGLWFEVQNSTDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLT 289
           PLNDGLWFEVQNS D +LKEF+IPQN YRAVLEVYVSFHENDEFWYSNLPN+YI ANNL+
Sbjct: 186 PLNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLS 245

Query: 290 DTPGNGAFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPF 349
            TPGNG FREV+V LDGE+VGAVWPFTVI+TGG+NPLLWRPIT IGSF+LP+YDIE+TPF
Sbjct: 246 GTPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPF 305

Query: 350 LGHLLDGKVHNFGFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNF 409
           LG +LDGK H FGF VT+ALNVWY++ANLHLWLD  S KTEGKL  H      +S   +F
Sbjct: 306 LGKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDF 365

Query: 410 TGPNGTFLTKVNRSLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFN 469
            G NGTFLT+ +RS+SSTGWVKSS+G ITT+S QD  YSNSMV+G +G+ Q VNQ+I FN
Sbjct: 366 KGLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKIIFN 425

Query: 470 SSVYAKQESSHVYSFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFIS 529
            SVY    SS+V+S  S KTFPLY+Y+D L +GNGTY  I N+ LGF EKK+ GLG   S
Sbjct: 426 DSVYINLPSSYVHSLTSHKTFPLYLYTDFLGQGNGTYLLITNVDLGFIEKKS-GLGFSNS 485

Query: 530 SLKNLQNGQGIMVVKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICG 589
           SL+NL++ +G MVVK NLV  GL STQQ YRY     CY RNISSSNYTILYD V + C 
Sbjct: 486 SLRNLRSAEGNMVVKNNLVVSGLESTQQIYRYDGGKFCYFRNISSSNYTILYDKVGSKCN 545

Query: 590 NQ-LESLESLRFRRGPIPARRAF 612
            + L +L+ +  R  P  AR  F
Sbjct: 546 KKSLSNLDFVLSRLWPFGARMNF 565

BLAST of HG10013705 vs. ExPASy TrEMBL
Match: A0A0A0L222 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G627190 PE=4 SV=1)

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 539/621 (86.80%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 3   SSSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTK 62
           +SS L  + L+ L  LLLPFHSNANLHKTRLR+SDL S+ D+TTTADH+TPPTVFFEV+K
Sbjct: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61

Query: 63  PIETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFD 122
           PI TPK+KPCSLL+L HDF +TYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFD
Sbjct: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121

Query: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTG 182
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLL KNQTLAVYLGNL+DKTYTG
Sbjct: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181

Query: 183 IYHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNS 242
           IY+V+I IHFYPEE+GFG NGVYS+KL S Y SDSRADLILPISRNLPLNDGLWFEVQNS
Sbjct: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 241

Query: 243 TDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLV 302
           TDV+ KEF+IPQNVYRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLV
Sbjct: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 303 DLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFG 362
           DLDGEIVGAVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGKVHNFG
Sbjct: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361

Query: 363 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNR 422
           FRVTHALNVW+INANLHLWLDDNS KTE KLLNHIVSSP +S+DLNFTGP+GTFLTKV R
Sbjct: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421

Query: 423 SLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVY 482
           S+SSTGWVKSSFGIITT SNQDLSYSNSM MGNNGSSQTVNQEIQFN+SVYAK+ESSHVY
Sbjct: 422 SVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481

Query: 483 SFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMV 542
           S KSLKTFPLYMYS+T DE NG+YQSIANLTLGFNEKKTDGLG  ISSLKN+QNGQG++V
Sbjct: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541

Query: 543 VKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRR 602
           VKG+LVT GLGSTQQDYRYHDD+QCYSRNISSSNYTIL+DNVKN CG +  S ESLRFRR
Sbjct: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601

Query: 603 GPIPARRAFLDSNLHDNNGHV 624
            PIPARRAFLDSNL  NNG V
Sbjct: 602 WPIPARRAFLDSNLFVNNGRV 622

BLAST of HG10013705 vs. ExPASy TrEMBL
Match: A0A5D3DX64 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001000 PE=4 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 539/619 (87.08%), Postives = 579/619 (93.54%), Query Frame = 0

Query: 5   SSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKPI 64
           +S S L LISL  LLLPFHS+A+LHKTRLRQSDL S+S ++TTADH+TPPTVFFEVTKPI
Sbjct: 2   ASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPI 61

Query: 65  ETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDRI 124
            TPKAKPCSLLVL HDF YTYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFDRI
Sbjct: 62  VTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRI 121

Query: 125 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGIY 184
           FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLL KNQTLAVYLGNL+DKTYTGIY
Sbjct: 122 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGIY 181

Query: 185 HVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNSTD 244
           +V+IHIHFYPEE+GFG NGVYS+KL S Y SDSRADLILPISRNLPLNDGLWFEVQNSTD
Sbjct: 182 NVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTD 241

Query: 245 VQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLVDL 304
           VQLKEF+IPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLVDL
Sbjct: 242 VQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDL 301

Query: 305 DGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFGFR 364
           DG+IV AVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGFR
Sbjct: 302 DGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFR 361

Query: 365 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNRSL 424
           VTHALNVW+INANLHLWLDDNS KTE KLLNHIVSSP +S+DLNFTG +GTFLTKV RS+
Sbjct: 362 VTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSV 421

Query: 425 SSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVYSF 484
           SS GWVKSSFG ITT SNQDLSYSN M+MGNNGSSQTVNQEI FNSSVYAKQESSHVYS 
Sbjct: 422 SSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSS 481

Query: 485 KSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 544
           K+LKTFPLYMYS+T+D+GNG+Y+SIANLTLGFNEKKTDGLGSFISSLKN+QNGQG+MVVK
Sbjct: 482 KTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVK 541

Query: 545 GNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRRGP 604
           GNLVT GLGSTQQDYRY+DD+QCYSR+ISSSNYTIL+DNVKN+CG +  S ESLRFRR P
Sbjct: 542 GNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRWP 601

Query: 605 IPARRAFLDSNLHDNNGHV 624
           IPARRA LDSNL  NNGHV
Sbjct: 602 IPARRASLDSNLFVNNGHV 620

BLAST of HG10013705 vs. ExPASy TrEMBL
Match: A0A1S3CJY5 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Cucumis melo OX=3656 GN=LOC103501812 PE=4 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 539/619 (87.08%), Postives = 579/619 (93.54%), Query Frame = 0

Query: 5   SSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKPI 64
           +S S L LISL  LLLPFHS+A+LHKTRLRQSDL S+S ++TTADH+TPPTVFFEVTKPI
Sbjct: 2   ASSSFLLLISLLLLLLPFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTKPI 61

Query: 65  ETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDRI 124
            TPKAKPCSLLVL HDF YTYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFDRI
Sbjct: 62  VTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRI 121

Query: 125 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGIY 184
           FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLL KNQTLAVYLGNL+DKTYTGIY
Sbjct: 122 FGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTGIY 181

Query: 185 HVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNSTD 244
           +V+IHIHFYPEE+GFG NGVYS+KL S Y SDSRADLILPISRNLPLNDGLWFEVQNSTD
Sbjct: 182 NVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTD 241

Query: 245 VQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLVDL 304
           VQLKEF+IPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLVDL
Sbjct: 242 VQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLVDL 301

Query: 305 DGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFGFR 364
           DG+IV AVWPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGFR
Sbjct: 302 DGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFGFR 361

Query: 365 VTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNRSL 424
           VTHALNVW+INANLHLWLDDNS KTE KLLNHIVSSP +S+DLNFTG +GTFLTKV RS+
Sbjct: 362 VTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTRSV 421

Query: 425 SSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVYSF 484
           SS GWVKSSFG ITT SNQDLSYSN M+MGNNGSSQTVNQEI FNSSVYAKQESSHVYS 
Sbjct: 422 SSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVYSS 481

Query: 485 KSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVVK 544
           K+LKTFPLYMYS+T+D+GNG+Y+SIANLTLGFNEKKTDGLGSFISSLKN+QNGQG+MVVK
Sbjct: 482 KTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMVVK 541

Query: 545 GNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRRGP 604
           GNLVT GLGSTQQDYRY+DD+QCYSR+ISSSNYTIL+DNVKN+CG +  S ESLRFRR P
Sbjct: 542 GNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRRWP 601

Query: 605 IPARRAFLDSNLHDNNGHV 624
           IPARRA LDSNL  NNGHV
Sbjct: 602 IPARRASLDSNLFVNNGHV 620

BLAST of HG10013705 vs. ExPASy TrEMBL
Match: A0A6J1CHN7 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Momordica charantia OX=3673 GN=LOC111011511 PE=4 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.1e-306
Identity = 524/621 (84.38%), Postives = 562/621 (90.50%), Query Frame = 0

Query: 4   SSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKP 63
           +SSLSS   ISL  LLLPF+S+ANLHKTR R+ DLLS+   T    HT PPT+FFEVTKP
Sbjct: 2   ASSLSSPLFISLLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTKP 61

Query: 64  IETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 123
           IETPKAKPCS+L LQHDFGYTYGKSPVLADY+PPSHCSF+KFSKIVLEW ATCRGRQFDR
Sbjct: 62  IETPKAKPCSVLALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFDR 121

Query: 124 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGI 183
           IFGVWLGGVEILRSCTAEPRATGIVWTV+KDITRY+SLL KNQTLAVYLGNL+DKTYTGI
Sbjct: 122 IFGVWLGGVEILRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 181

Query: 184 YHVEIHIHFYPEEKGFGDN-GVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNS 243
           YHV IHIHFYPEEK FGDN G + +K ASGY  DS ADLI+PISR+LPLNDGLWFEVQ+S
Sbjct: 182 YHVRIHIHFYPEEKSFGDNGGRFLEKSASGY--DSGADLIVPISRSLPLNDGLWFEVQSS 241

Query: 244 TDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLV 303
           TDVQ+KEF+IPQNVYRAVLEVYVSFHENDEFWYSNLPNDYI ANNLTDTPGNG FREVLV
Sbjct: 242 TDVQVKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301

Query: 304 DLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFG 363
            LDG+IVGAVWPFTV+YTGGVNPLLWRPITGIGSFNLPSYDIE+TPFLG+LLDGK HNFG
Sbjct: 302 SLDGQIVGAVWPFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFG 361

Query: 364 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNR 423
           FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVS    SE+ NFTGP+GTF TKV R
Sbjct: 362 FRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTR 421

Query: 424 SLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVY 483
           S+SS GWVKSS GIITTQSNQD SYSNSM +G+NGSSQ VNQ+IQFNSSVYAKQ+SSHVY
Sbjct: 422 SVSSIGWVKSSRGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVY 481

Query: 484 SFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMV 543
           SFKSLKTFPLY+YSDT DEGNGTYQS+ANLTLGFNEKKTDG GSFISSLKNLQNGQG+MV
Sbjct: 482 SFKSLKTFPLYLYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMV 541

Query: 544 VKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRR 603
           VK +LVTRGLGSTQQDYRYHDD  CY RNISSSNYTIL+DNVKN CG + ES  SLRF R
Sbjct: 542 VKDHLVTRGLGSTQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGR 601

Query: 604 GPIPARRAFLDSNLHDNNGHV 624
            PIPARRAFLDS+L  N+ HV
Sbjct: 602 WPIPARRAFLDSSLQVNSVHV 620

BLAST of HG10013705 vs. ExPASy TrEMBL
Match: A0A6J1FLZ6 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita moschata OX=3662 GN=LOC111446428 PE=4 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 3.2e-302
Identity = 512/609 (84.07%), Postives = 559/609 (91.79%), Query Frame = 0

Query: 4   SSSLSSLFLISLFFLLLPFHSNANLHKTRLRQSDLLSDSDLTTTADHTTPPTVFFEVTKP 63
           +SSLSS  LISL F+LLPFHSNA+LH+T LR+ DL S+S+L  T +HTTPPT+FFEVTKP
Sbjct: 2   ASSLSSPLLISLLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTKP 61

Query: 64  IETPKAKPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 123
           IETP+A+PCSLL+LQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR
Sbjct: 62  IETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDR 121

Query: 124 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQTLAVYLGNLIDKTYTGI 183
           IFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLL KNQTLAVYLGNLIDKTYTGI
Sbjct: 122 IFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGI 181

Query: 184 YHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDGLWFEVQNST 243
           YHV+IHIHFYPEEK F     YS+KLA+GY  DSRADLILPISR+LPLNDGLWFEVQNST
Sbjct: 182 YHVKIHIHFYPEEKRF----QYSEKLAAGY--DSRADLILPISRSLPLNDGLWFEVQNST 241

Query: 244 DVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGNGAFREVLVD 303
           D+Q+KEF+IPQN YRAVLEVY+SFHENDEFWYSNLPNDYI ANNL+ TPGNG FREVLV+
Sbjct: 242 DIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLVN 301

Query: 304 LDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKVHNFGF 363
           LD EIVGA+WPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGK H+FGF
Sbjct: 302 LDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGF 361

Query: 364 RVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNGTFLTKVNRS 423
            VTHALNVWYINANLHLWLDD S KTEGKLLNHI+S    SEDLNFTGP+GTFLTK +RS
Sbjct: 362 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRS 421

Query: 424 LSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYAKQESSHVYS 483
           +SS+GWVKSS GIITTQSNQD SYSNSMVMGN+G+SQ VNQEIQFNSSVYAK+ES HVYS
Sbjct: 422 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVYS 481

Query: 484 FKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDGLGSFISSLKNLQNGQGIMVV 543
           FKSLKTFPLYMYS+T+DEGNG++QSIANLTLGFNEKK DGLGSFISSLKN+QNGQG+M V
Sbjct: 482 FKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTV 541

Query: 544 KGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNICGNQLESLESLRFRRG 603
           KG+LVTRGLGSTQQDYRYHDD  CYSRNISSSNYTILYD+V ++C  + ES ESLRFR+ 
Sbjct: 542 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKW 601

Query: 604 PIPARRAFL 613
           P  +RR FL
Sbjct: 602 PFLSRRVFL 604

BLAST of HG10013705 vs. TAIR 10
Match: AT3G14920.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 678.7 bits (1750), Expect = 4.4e-195
Identity = 352/598 (58.86%), Postives = 435/598 (72.74%), Query Frame = 0

Query: 4   SSSLSSLFLISLFFLLLPFHSNANLHKTRLR----QSDLLSDSDLTTTADHTTPPTVFFE 63
           +SSL  L      F +    + ++LH+TR R    Q   L  S L      T  PT +FE
Sbjct: 2   TSSLPPLIFFFTVFFVHSLSAVSDLHETRSRFKPPQFSPLFFSSLPQNV--TKSPTRYFE 61

Query: 64  VTKP--IETPKA-KPCSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATC 123
           V KP     P A +PCS  +L HDFGYTY K PVL++Y+ PSHCS  +FSKIVLE+K+T 
Sbjct: 62  VQKPPVPNLPTAQQPCSYQILHHDFGYTYAKPPVLSNYTLPSHCSSREFSKIVLEFKSTS 121

Query: 124 RGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQT--LAVYLGN 183
           +GRQFDRIFGVWL GVEILRSCTAEPR  GIVW+V+KD+T+Y+SLL+KN+T  L+VYLGN
Sbjct: 122 QGRQFDRIFGVWLDGVEILRSCTAEPRPNGIVWSVEKDVTKYHSLLVKNETQILSVYLGN 181

Query: 184 LIDKTYTGIYHVEIHIHFYPEEKGFGDNGVYSKKLASGYGSDSRADLILPISRNLPLNDG 243
           LIDKTYTG+YHV++  HFY  E    D         SGY S S+AD+ILPISRNLPLNDG
Sbjct: 182 LIDKTYTGVYHVDVIFHFYQSESNLQD--------VSGY-SSSKADMILPISRNLPLNDG 241

Query: 244 LWFEVQNSTDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNLTDTPGN 303
           LWFE+ NS D + KEF+IP+NVYRAVLEVYVSFHENDEFWY NLPNDY+ ANNL+   GN
Sbjct: 242 LWFEIVNSNDTKYKEFEIPRNVYRAVLEVYVSFHENDEFWYGNLPNDYVTANNLS-VAGN 301

Query: 304 GAFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLL 363
           G FREV+V LDG+I GAVWPF V++TGG+NPLLWRPIT IGSF+LPSYDIE+TPFLG LL
Sbjct: 302 GPFREVVVSLDGDIAGAVWPFPVVFTGGINPLLWRPITAIGSFDLPSYDIEITPFLGSLL 361

Query: 364 DGKVHNFGFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLNFTGPNG 423
           DGK H  GF VT+ALNVWYI+ANLHLWLD      EGK+L+   SS  IS   +F G NG
Sbjct: 362 DGKTHKVGFSVTNALNVWYIDANLHLWLDQEKEIVEGKVLDFSRSSLEISSVSDFKGLNG 421

Query: 424 TFLTKVNRSLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQFNSSVYA 483
            F TK  RS++S G VKSS G I T +NQ+ SY N MV+G +G+ Q ++Q IQ +  ++A
Sbjct: 422 NFTTKAKRSITSVGLVKSSHGDIITNANQEFSYENKMVLGKDGNLQIIDQLIQADDRIHA 481

Query: 484 KQESSHVYSFKSLKTFPLYMYSDTLDEGNGTYQSIANLTLGFNEKKTDG----LGSFISS 543
           K+ S  +Y+ KS+K+FP Y+ SDTL++ N TY ++AN+++ FNE++++     + +F S 
Sbjct: 482 KRASREIYAAKSIKSFPFYLDSDTLEQQNNTYLAVANVSMAFNEERSESDKGLMRTFKSK 541

Query: 544 LKNLQNGQGIMVVKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKNIC 589
           L+N Q GQG+MVVK NLV  G GSTQQ Y Y    QCY RNISS NYTILYD V+++C
Sbjct: 542 LENKQEGQGVMVVKNNLVVSGYGSTQQVYNYVGSDQCYFRNISSYNYTILYDKVESVC 587

BLAST of HG10013705 vs. TAIR 10
Match: AT5G05480.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 382.9 bits (982), Expect = 5.0e-106
Identity = 215/558 (38.53%), Postives = 311/558 (55.73%), Query Frame = 0

Query: 54  PTVFFEVTKPIETPKAKP-CSLLVLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEW 113
           P  + E+ +P+ + +  P CS ++ +H F  T  + P    Y+PPS C    +S +VL+ 
Sbjct: 46  PQEYEELRRPLPSDQLTPSCSHVLFRHSFANTINRPPFTTPYTPPSSCISPPWSYVVLDL 105

Query: 114 KATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLLKNQ-TLAVY 173
           +A   G Q+DRI G+WLGGVE+LR+ TAEP  +GI W V+KD++RY+SL +++   + + 
Sbjct: 106 RAASSGDQYDRISGLWLGGVELLRTSTAEPSPSGIFWNVRKDVSRYSSLFMRSDLNVTMM 165

Query: 174 LGNLIDKTYTGIYHVEIHIHFYPEEKGFGDNGVYSKKLA---SGYGSDSRADLILPISRN 233
           L N+++  YTGIYH+ + + FY E      N   S +L    S     + ADLI+P+   
Sbjct: 166 LENIVNDVYTGIYHINVTLIFY-EFNPIASNLKISNRLGFVDSQKRDQTPADLIIPVCDG 225

Query: 234 LPLNDGLWFEVQNSTDVQLKEFQIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIFANNL 293
              N G WF ++N  +   K  QIP N  + VLE+YVSFH NDEFWYSN PN YI  NNL
Sbjct: 226 --GNRGFWFMIENPRETYSKGIQIPSNTRQIVLELYVSFHGNDEFWYSNPPNSYIRTNNL 285

Query: 294 TDTPGNGAFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTP 353
           T   GNGA+REV V +DG  VG+  PF VI+TGG+NPL W P+  IG+FNLPSYD++LTP
Sbjct: 286 TTGRGNGAYREVFVKIDGRYVGSEVPFPVIFTGGINPLFWEPVVAIGAFNLPSYDMDLTP 345

Query: 354 FLGHLLDGKVHNFGFRVTHALNVWYINANLHLWLDDNSAKTEGKLLNHIVSSPFISEDLN 413
           FLG LLDGK H F F V   ++ W ++ANLHLWLD  S+  E     +   S  +     
Sbjct: 346 FLGLLLDGKSHEFAFGVNDGISYWLVDANLHLWLDHGSSNVEAGSRLYDSPSRHMMRQEQ 405

Query: 414 FTGPNGTFLTKVNRSLSSTGWVKSSFGIITTQSNQDLSYSNSMVMGNNGSSQTVNQEIQF 473
               +G+F  +   S    GWV+SS G +TT         + +    NG+ + V Q ++ 
Sbjct: 406 LEQLDGSFKVEAEVSSEYDGWVRSSKGNLTTMVKSMFKVDSLVRFEKNGTYKRVEQRVKT 465

Query: 474 NSSVYAKQESSH-VYSFKSLKTFPLYMYSDT---LDEGNGTYQSIANLTLGFNEKKTDGL 533
              V    ES   V      +T+PL + + T   L     TY  + N++   NE  +D  
Sbjct: 466 ERIVEVTSESGKPVNRVVHQRTYPLTVITSTLRGLTNDKDTYMLVTNVSHALNETHSD-- 525

Query: 534 GSFISSLKNLQNGQGIMVVKGNLVTRGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNV 593
           G  +  + + Q+ +G M V+ + V  G  ST+Q   Y D+  CYSR I ++N  I  D+ 
Sbjct: 526 GEALIQVYSRQDSEGWMEVEDHNVLAGEASTKQSLSYIDEFSCYSRTIVAANGEIAQDSS 585

Query: 594 KNICG----NQLESLESL 599
            + C     ++L ++E L
Sbjct: 586 SDSCTSSSFSELNTIEQL 598