HG10013677 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013677
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionD-amino-acid transaminase, chloroplastic-like
LocationChr02: 3698653 .. 3701232 (+)
RNA-Seq ExpressionHG10013677
SyntenyHG10013677
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCTTTGAACTCTCCTTCTAATGAAACTGAAGGTGGGGTTTTTTTTTTTTTTTTTTTTTTCATGTGGGGTTTGAGATTTTTGTGGGTTTTTCTTGTCTGGTCTTTTGTTGTTGTTAATATGATTGTTTCCGCTTGTGTGATGATGCATTAGCGATTGGAAATGGAACTGAAACTGACTTGAAGGTTCATGTGTTCTCATCATCTTTCGAGGTAAGTTCTCGAAACCCTATGTTTGATTGATCGTTATTACCCCTTACATGTTTGTATTGTATGTTTTGCATTTGGCATGGAGAAAGTTTGAGATTGTAGAGGCTGTGAGCTGGTACTGAATCAATTTACTAATAGAAAGGATTGGATTATGGTTTCGGTAAATTTGTTCTCTATATGGTTAAACTTCCATGGCTCGAGTCTTAGGACCTGTTGGAACCTGTTTGAGTACAAAAAAAGTGTGATAGAACCCAAATGAATTGAAATATAAATATTATAAGAACTAGATGATAAACTAAGGGATATTTGGAGTGCTGAATTGGTTATTATAGTCCGTTATTTATAATAGTCTATAAATTATAATAATTTATATTTGGGGTGCTGACTATTTTAGTATGAGTTATAATATTCTGTGTTGGGAGTGCACACTATTTTAGTTTGGGTAGGAAATAGTAAATACGGTAATAAAGAGAAATTGGAAGTAGTAATACTGTAGTTAATTGTAAGTTATAATGTTTAAAAACACCAACTATTATAATTGAGCTCCAAACATGGAGTGGGCTCCACCCACTTGAACCCACTCCTCTTGAGATATCTCTTAAGTCCCAATTCACTAACCAAAATAATGCATTTCTTTCTCCTTTTCCGCTCGCTCTCTATTTATAGCCAAATAACATAACATATCAAAGTGTTTTTAAGCTTTTAGAAAGTCATTGCTAAACAGGTCCTTAAACTAGTTTAACTTATTCAATAGTAGGGGTGCAGCTTTGAAATTAATTGAACTGAGTTAGTTTGGTTGGTTTGAAGTAGGTTGGTTGGTTTGAAGTTTGAATTGGGATTATCCTCACTTGTTGGTTTGGTCTTATTAAGTATATTACCAACAGTTAGTCGGTTAGCACGGTCTGAATAGATTTTGTAGTTTGGTGGCCAGTTTTGGTGGCGCAGGCAGTTTGGTTCTTTTATAAGAACTGATTTTTCTTTCTTTTTCCCTCGGTAAATTGACTTACTTACGCCCAAATTCGATAGATGTTGATGTATTGTCACCAAAAGTTTATGGATTGGTTGGTGAAGTTGGGAGACATTATTCTATGAGATTATTTTGGAGACATTTTTCTAGAGTAGGAAAGAGTAATTTGATGTAGTTGAGTTTTCTAATGGCTTTCGTAAAGTATCATTGTTGCAATTATTTTGCTCTTCCTTTCAAACAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCTATGTATTCCAGCATTTTTGGTGGAATTATTACAGATCCGGCTATGATGGTGATTCCTATTGATGACCACATGGTTCATCGAGGCCATGGCGTGTTTGATACAGCCATTATTTTAAATGGGTTGGTACATGGAATATTTCATCAAATTTATTTCAGTTCTTTTAAAATTTCTCAAGCTGCTGAACTGTTACTTCCTTACCAACTGTCCTCGTTATAAAGCCCTTGTTTTGGTTCTTAACAGACATTTGTATGAGTTGGATGCCCACCTCGACCGCTTCCTAAGATCAGCTTCAAAAGCGAAGATCTCTTCTCCCTTCCCACTGTCGATTCTTCGGAGCATTCTTATCCAATTGACAGCAGCATCACAGCTCAAGAAAGGCACCTTAAGATACTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCTGGAAATGCGAAATCTGCATTCTATGCGGTAGCCATTGATGAGGACTTCTCTCAGTGCAAAGAGGGGGTTAAAACGATAACATCCACGGTCCCGATGAAGACGCCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCATTTATAACCAATGAAAAGGAGCTTATCTTGCCTTCTTTCGACAAGGTTCTCTCGGGGTGCACCGCACTGCGTCTTCTTAAGCTGGCTCCAAAGCTGGTTAAGGATGGGAAGTTGAAGAGTGTAGGAACTGCAAACCTTACGGTTAAGGAAGCCAAGGGCGCTGCTGAAATGATGTTTGTAGGAAGCACACTTCCGCTACTGCCAATAATCTCATGGGATGAAGAACCTATTGGAGATGGTAACATCTATTGTCTGATATTAACTTCTGTTAGAGTGACAACGAATGTAATGTTCTTATAATCAACTCCATTCATTCTTTGCTACAGGAAGCGTCGGGGAACTGACGATGGCGCTATCGGATCTGCTTTGGGACGATATGGTCTCGGGTCCTGAAACAGAGAGAATACCAGTTTCATACACATTGTGA

mRNA sequence

ATGGAGGCTTTGAACTCTCCTTCTAATGAAACTGAAGCGATTGGAAATGGAACTGAAACTGACTTGAAGGTTCATGTGTTCTCATCATCTTTCGAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCTATGTATTCCAGCATTTTTGGTGGAATTATTACAGATCCGGCTATGATGGTGATTCCTATTGATGACCACATGGTTCATCGAGGCCATGGCGTGTTTGATACAGCCATTATTTTAAATGGACATTTGTATGAGTTGGATGCCCACCTCGACCGCTTCCTAAGATCAGCTTCAAAAGCGAAGATCTCTTCTCCCTTCCCACTGTCGATTCTTCGGAGCATTCTTATCCAATTGACAGCAGCATCACAGCTCAAGAAAGGCACCTTAAGATACTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCTGGAAATGCGAAATCTGCATTCTATGCGGTAGCCATTGATGAGGACTTCTCTCAGTGCAAAGAGGGGGTTAAAACGATAACATCCACGGTCCCGATGAAGACGCCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCATTTATAACCAATGAAAAGGAGCTTATCTTGCCTTCTTTCGACAAGGTTCTCTCGGGGTGCACCGCACTGCGTCTTCTTAAGCTGGCTCCAAAGCTGGTTAAGGATGGGAAGTTGAAGAGTGTAGGAACTGCAAACCTTACGGTTAAGGAAGCCAAGGGCGCTGCTGAAATGATGTTTGTAGGAAGCACACTTCCGCTACTGCCAATAATCTCATGGGATGAAGAACCTATTGGAGATGGAAGCGTCGGGGAACTGACGATGGCGCTATCGGATCTGCTTTGGGACGATATGGTCTCGGGTCCTGAAACAGAGAGAATACCAGTTTCATACACATTGTGA

Coding sequence (CDS)

ATGGAGGCTTTGAACTCTCCTTCTAATGAAACTGAAGCGATTGGAAATGGAACTGAAACTGACTTGAAGGTTCATGTGTTCTCATCATCTTTCGAGTTGCTCGAAAAACTTCACGAGAAATGGAGTTTGGTGAAGAAGAAACCATATCCAGCTATGTATTCCAGCATTTTTGGTGGAATTATTACAGATCCGGCTATGATGGTGATTCCTATTGATGACCACATGGTTCATCGAGGCCATGGCGTGTTTGATACAGCCATTATTTTAAATGGACATTTGTATGAGTTGGATGCCCACCTCGACCGCTTCCTAAGATCAGCTTCAAAAGCGAAGATCTCTTCTCCCTTCCCACTGTCGATTCTTCGGAGCATTCTTATCCAATTGACAGCAGCATCACAGCTCAAGAAAGGCACCTTAAGATACTGGTTAAGTGCAGGACCTGGAGATTTCTTGCTCACGCCTGCTGGAAATGCGAAATCTGCATTCTATGCGGTAGCCATTGATGAGGACTTCTCTCAGTGCAAAGAGGGGGTTAAAACGATAACATCCACGGTCCCGATGAAGACGCCTCAGTTTGCAACAATGAAGAATGTGAACTACCTACCAAATGTACTAGCCAAAATGGAAGCTGAAGAGAAAGGGGCATTTGCTTCCATTTGGGTTGATGAAGAAGGCTATATTGCAGAAGGTCCAAATGTGAATGTTGCATTTATAACCAATGAAAAGGAGCTTATCTTGCCTTCTTTCGACAAGGTTCTCTCGGGGTGCACCGCACTGCGTCTTCTTAAGCTGGCTCCAAAGCTGGTTAAGGATGGGAAGTTGAAGAGTGTAGGAACTGCAAACCTTACGGTTAAGGAAGCCAAGGGCGCTGCTGAAATGATGTTTGTAGGAAGCACACTTCCGCTACTGCCAATAATCTCATGGGATGAAGAACCTATTGGAGATGGAAGCGTCGGGGAACTGACGATGGCGCTATCGGATCTGCTTTGGGACGATATGGTCTCGGGTCCTGAAACAGAGAGAATACCAGTTTCATACACATTGTGA

Protein sequence

MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYTL
Homology
BLAST of HG10013677 vs. NCBI nr
Match: XP_038900252.1 (D-amino-acid transaminase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 663.7 bits (1711), Expect = 8.8e-187
Identity = 332/347 (95.68%), Postives = 340/347 (97.98%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           MEALNSPSNET+AIGNG+ETDLKVHVFSSSFELLEKLH+KWSLVKKKPYPAMYSSIFGGI
Sbjct: 1   MEALNSPSNETQAIGNGSETDLKVHVFSSSFELLEKLHQKWSLVKKKPYPAMYSSIFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFP SI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPRSI 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LRSILIQLTA SQLKKGTLRYWLSAGPGDFLLTPA NAKSAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNAKSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+TVPMKTPQFATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTVPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELI PSFDKVLSGCTALRLLK+APKLV+DGKLKSVGTANLTVKEAKGAAEMMFVGSTL
Sbjct: 241 EKELIFPSFDKVLSGCTALRLLKIAPKLVEDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           PLLPIISWD EPIGDGSVGELTMALSDLLWDDMVSGPETERIPV YT
Sbjct: 301 PLLPIISWDGEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVPYT 347

BLAST of HG10013677 vs. NCBI nr
Match: XP_004146044.1 (D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypothetical protein Csa_012285 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 4.2e-181
Identity = 323/347 (93.08%), Postives = 334/347 (96.25%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           MEALNS S+E +AIGNG+ETDLKV VFSSS ELLEKLHEKWSLV KKPYPAMYSS+FGGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LRSILIQLTA SQLKKGTLRYWLSAGPGDFLLTPA NA SAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNANSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITST+PMKTPQFATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELILPSFDK+LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAK AAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYT 347

BLAST of HG10013677 vs. NCBI nr
Match: XP_038900251.1 (D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 642.1 bits (1655), Expect = 2.7e-180
Identity = 323/342 (94.44%), Postives = 331/342 (96.78%), Query Frame = 0

Query: 7   PSN-ETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGIITDPA 66
           P+N    AIGNG+ETDLKVHVFSSSFELLEKLH+KWSLVKKKPYPAMYSSIFGGIITDPA
Sbjct: 73  PANYNRSAIGNGSETDLKVHVFSSSFELLEKLHQKWSLVKKKPYPAMYSSIFGGIITDPA 132

Query: 67  MMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSIL 126
           MMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFP SILRSIL
Sbjct: 133 MMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPRSILRSIL 192

Query: 127 IQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTV 186
           IQLTA SQLKKGTLRYWLSAGPGDFLLTPA NAKSAFYAVAID+DFSQCKEGVK IT+TV
Sbjct: 193 IQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNAKSAFYAVAIDDDFSQCKEGVKAITTTV 252

Query: 187 PMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELI 246
           PMKTPQFATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELI
Sbjct: 253 PMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELI 312

Query: 247 LPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPI 306
            PSFDKVLSGCTALRLLK+APKLV+DGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPI
Sbjct: 313 FPSFDKVLSGCTALRLLKIAPKLVEDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPI 372

Query: 307 ISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           ISWD EPIGDGSVGELTMALSDLLWDDMVSGPETERIPV YT
Sbjct: 373 ISWDGEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVPYT 414

BLAST of HG10013677 vs. NCBI nr
Match: XP_008463704.1 (PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 639.8 bits (1649), Expect = 1.4e-179
Identity = 321/347 (92.51%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           MEALNS S+E +AIGNG+ETDLKV VFSSS ELLEKLHEKWSLV+KKPYPAMYSSI GGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LRSILIQLT  SQLKKGTLRYWLSAGPGDFLLTPA +A SAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRSILIQLTTVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKV 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+T+PMKTP FATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTIPMKTPLFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELILPSFDK+LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAKGAAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 301 PLLPIISWDEEPIGDGHVGELTMALSDLLWDDMVSGPETERIPVSYT 347

BLAST of HG10013677 vs. NCBI nr
Match: KAA0066805.1 (hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK27952.1 hypothetical protein E5676_scaffold384G001560 [Cucumis melo var. makuwa])

HSP 1 Score: 624.8 bits (1610), Expect = 4.5e-175
Identity = 312/335 (93.13%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 13  AIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGIITDPAMMVIPID 72
           +IGNG+ETDLKV VFSSS ELLEKLHEKWSLV+KKPYPAMYSSI GGIITDPAMMVIPID
Sbjct: 405 SIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPID 464

Query: 73  DHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQLTAAS 132
           DHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SILRSILIQLT  S
Sbjct: 465 DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVS 524

Query: 133 QLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVPMKTPQF 192
           QLKKGTLRYWLSAGPGDFLLTPA +A SAFYAVAID+DFSQCKEGVK IT+T+PMKTP F
Sbjct: 525 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLF 584

Query: 193 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKV 252
           ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDK+
Sbjct: 585 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 644

Query: 253 LSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 312
           LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP
Sbjct: 645 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 704

Query: 313 IGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           IGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 705 IGDGHVGELTMALSDLLWDDMVSGPETERIPVSYT 739

BLAST of HG10013677 vs. ExPASy Swiss-Prot
Match: Q8L493 (D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 3.0e-97
Identity = 182/320 (56.88%), Postives = 236/320 (73.75%), Query Frame = 0

Query: 29  SSFELLEKLHEKWSLVK-KKPYPAMYSSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAI 88
           SS+E+ E+L     L +  + + AMYSS+  GI TDPA MV+P+DDHMVHRGHGVFDTA+
Sbjct: 58  SSYEVGERL----KLARGGQQFLAMYSSVVDGITTDPAAMVLPLDDHMVHRGHGVFDTAL 117

Query: 89  ILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQLTAASQLKKGTLRYWLSAGP 148
           I+NG+LYELD HLDR LRSAS AKI  PF    ++ ILIQ  + S  + G+LRYWLSAGP
Sbjct: 118 IINGYLYELDQHLDRILRSASMAKIPLPFDRETIKRILIQTVSVSGCRDGSLRYWLSAGP 177

Query: 149 GDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVPMKTPQFATMKNVNYLPNVLAK 208
           GDFLL+P+   K   YA+ I  +F+    GVK +TS++P+K P+FAT+K+VNYLPNVL++
Sbjct: 178 GDFLLSPSQCLKPTLYAIVIKTNFAINPIGVKVVTSSIPIKPPEFATVKSVNYLPNVLSQ 237

Query: 209 MEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN-EKELILPSFDKVLSGCTALRLLKLAP 268
           MEAE KGA+A IWV ++G+IAEGPN+NVAF+ N  KEL++P FD VLSGCTA R L LA 
Sbjct: 238 MEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNGGKELVMPRFDNVLSGCTAKRTLTLAE 297

Query: 269 KLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALS 328
           +LV  G LK+V   ++TV++ K A EMM +GS +P+ P+I WDEE IG+G  G +  AL 
Sbjct: 298 QLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGIPIRPVIQWDEEFIGEGKEGPIAKALL 357

Query: 329 DLLWDDMVSGPETERIPVSY 347
           DLL +DM SGP + R+ V Y
Sbjct: 358 DLLLEDMRSGPPSVRVLVPY 373

BLAST of HG10013677 vs. ExPASy Swiss-Prot
Match: Q58414 (Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=ilvE PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 72/276 (26.09%), Postives = 129/276 (46.74%), Query Frame = 0

Query: 59  GIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPL 118
           G   D     + + DH +  G GVF+     +G ++ L  H+DR   SA    I  P   
Sbjct: 7   GKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTK 66

Query: 119 SILRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGV 178
             +  ++++    + L+   +R  ++ G GD  L P    K   + +AI       ++G+
Sbjct: 67  EEMIDVVLETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGI 126

Query: 179 KTITSTV---PMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNV 238
           + IT +V   P+     A +K++NYL +VLAK++A   G   +  +D++G++ EG   N+
Sbjct: 127 RAITVSVRRLPVDVLNPA-VKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNI 186

Query: 239 AFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMF 298
             + N      P +  +L G T   ++KLA    K+  ++ V    LT+ +   A E+  
Sbjct: 187 FIVKNGVLKTPPVYQSILKGITRDVVIKLA----KEEGIEVV-EEPLTLHDLYTADELFI 246

Query: 299 VGSTLPLLPIISWDEEPIGDGSVGELTMALSDLLWD 332
            G+   ++P+   D   I +  VGE+T  L +   D
Sbjct: 247 TGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKD 276

BLAST of HG10013677 vs. ExPASy Swiss-Prot
Match: O29329 (Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=ilvE PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.1e-18
Identity = 74/283 (26.15%), Postives = 128/283 (45.23%), Query Frame = 0

Query: 69  IPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQL 128
           + I DH    G GVF+     NG ++ L  H+DR   SA    +  P        I+++ 
Sbjct: 18  VSIFDHGFLYGDGVFEGIRAYNGRVFRLKEHIDRLYDSAKAIDLEIPITKEEFMEIILET 77

Query: 129 TAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQC-----KEGVKTITS 188
              + L+   +R  ++ G GD  L P      +   + I + + +      ++G+  IT 
Sbjct: 78  LRKNNLRDAYIRPIVTRGIGDLGLDPRKCQNPSI--IVITKPWGKLYGDLYEKGLTAITV 137

Query: 189 TVPMKTPQF--ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNE 248
            V   +       +K++NYL N+LAK+EA  KG   +I++D  GY++EG   N+ F+   
Sbjct: 138 AVRRNSFDALPPNIKSLNYLNNILAKIEANAKGGDEAIFLDRNGYVSEGSGDNI-FVVKN 197

Query: 249 KELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLP 308
             +  P     L G T   ++++  +L    K  ++G  +L       A E+   G+   
Sbjct: 198 GAITTPPTINNLRGITREAVIEIINRLGIPFKETNIGLYDLYT-----ADEVFVTGTAAE 257

Query: 309 LLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPV 345
           + PI+  D   IGDG  GE+T      L ++     E+E +P+
Sbjct: 258 IAPIVVIDGRKIGDGKPGEITRK----LMEEFSKLTESEGVPI 288

BLAST of HG10013677 vs. ExPASy Swiss-Prot
Match: O07597 (D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.4e-15
Identity = 67/259 (25.87%), Postives = 122/259 (47.10%), Query Frame = 0

Query: 69  IPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQL 128
           I ++D     G G+++   +  G L+ L  H +RF RSA++  IS PF +  L   L +L
Sbjct: 17  IDLEDRGYQFGDGIYEVIRVYKGVLFGLREHAERFFRSAAEIGISLPFSIEDLEWDLQKL 76

Query: 129 TAASQLKKGTLRYWLSAG--PGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVP 188
              + + +G +    + G  P           ++  Y   + +   +   GV  IT    
Sbjct: 77  VQENAVSEGAVYIQTTRGVAPRKHQYEAGLEPQTTAYTFTVKKPEQEQAYGVAAITDE-D 136

Query: 189 MKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELIL 248
           ++  +   +K++N L NV+ K  A E GAF +I +  +G + EG + NV  + N      
Sbjct: 137 LRWLR-CDIKSLNLLYNVMTKQRAYEAGAFEAILL-RDGVVTEGTSSNVYAVINGTVRTH 196

Query: 249 PSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPII 308
           P+   +L+G T + +L L   + K+G    +    ++ +E K A E+    +T  ++P++
Sbjct: 197 PANRLILNGITRMNILGL---IEKNG--IKLDETPVSEEELKQAEEIFISSTTAEIIPVV 256

Query: 309 SWDEEPIGDGSVGELTMAL 326
           + D + IG G  G +T  L
Sbjct: 257 TLDGQSIGSGKPGPVTKQL 267

BLAST of HG10013677 vs. ExPASy Swiss-Prot
Match: P63511 (D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=dat PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.8e-12
Identity = 69/279 (24.73%), Postives = 120/279 (43.01%), Query Frame = 0

Query: 52  MYSSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAK 111
           M      G    P+   +  +D     G G+++   + NG L+ +  H +RFLRSA++  
Sbjct: 1   MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIG 60

Query: 112 ISSPFPLSILRSILIQLTAASQLKKGTLRYWLSAGPGD---FLLTPAGNAKSAFYAVAID 171
           +   + +  L  +  +L   +Q++ G +    + G  +      TP        Y  + D
Sbjct: 61  LDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYD 120

Query: 172 EDFSQCKEGVKTITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIA 231
             +   + GV  +  TV         +K++N L NVLAK  A +  A  +I    E  + 
Sbjct: 121 RPYDHLENGVNGV--TVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGE-TVT 180

Query: 232 EGPNVNVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAK 291
           EG + N   I +      P  + +L+G T + + K+A       K ++      TV   K
Sbjct: 181 EGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEET-----FTVDFLK 240

Query: 292 GAAEMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALSD 328
            A E++   ++  + P+I  D EPI DG VG +T  L +
Sbjct: 241 NADEVIVSSTSAEVTPVIKLDGEPINDGKVGPITRQLQE 271

BLAST of HG10013677 vs. ExPASy TrEMBL
Match: A0A0A0KZK6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.0e-181
Identity = 323/347 (93.08%), Postives = 334/347 (96.25%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           MEALNS S+E +AIGNG+ETDLKV VFSSS ELLEKLHEKWSLV KKPYPAMYSS+FGGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVNKKPYPAMYSSVFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LRSILIQLTA SQLKKGTLRYWLSAGPGDFLLTPA NA SAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRSILIQLTAVSQLKKGTLRYWLSAGPGDFLLTPAVNANSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITST+PMKTPQFATMKNVNYLPNVLAK+EAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITSTIPMKTPQFATMKNVNYLPNVLAKLEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELILPSFDK+LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAK AAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 301 PLLPIISWDEEPIGDGRVGELTMALSDLLWDDMVSGPETERIPVSYT 347

BLAST of HG10013677 vs. ExPASy TrEMBL
Match: A0A1S3CJV5 (D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103501786 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.6e-180
Identity = 321/347 (92.51%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           MEALNS S+E +AIGNG+ETDLKV VFSSS ELLEKLHEKWSLV+KKPYPAMYSSI GGI
Sbjct: 1   MEALNSSSSEIQAIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMMVIPIDDHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SI
Sbjct: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSI 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LRSILIQLT  SQLKKGTLRYWLSAGPGDFLLTPA +A SAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRSILIQLTTVSQLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKV 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           IT+T+PMKTP FATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN
Sbjct: 181 ITTTIPMKTPLFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELILPSFDK+LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAKGAAEMMFVGSTL
Sbjct: 241 EKELILPSFDKILSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           PLLPIISWDEEPIGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 301 PLLPIISWDEEPIGDGHVGELTMALSDLLWDDMVSGPETERIPVSYT 347

BLAST of HG10013677 vs. ExPASy TrEMBL
Match: A0A5D3DWQ3 (Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001560 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.2e-175
Identity = 312/335 (93.13%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 13  AIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGIITDPAMMVIPID 72
           +IGNG+ETDLKV VFSSS ELLEKLHEKWSLV+KKPYPAMYSSI GGIITDPAMMVIPID
Sbjct: 405 SIGNGSETDLKVPVFSSSLELLEKLHEKWSLVEKKPYPAMYSSILGGIITDPAMMVIPID 464

Query: 73  DHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQLTAAS 132
           DHMVHRGHGVFDTAIILNG+LYELDAH+DRFLRSASKAKIS PFP SILRSILIQLT  S
Sbjct: 465 DHMVHRGHGVFDTAIILNGYLYELDAHIDRFLRSASKAKISPPFPRSILRSILIQLTTVS 524

Query: 133 QLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVPMKTPQF 192
           QLKKGTLRYWLSAGPGDFLLTPA +A SAFYAVAID+DFSQCKEGVK IT+T+PMKTP F
Sbjct: 525 QLKKGTLRYWLSAGPGDFLLTPAVDANSAFYAVAIDDDFSQCKEGVKVITTTIPMKTPLF 584

Query: 193 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKV 252
           ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDK+
Sbjct: 585 ATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITNEKELILPSFDKI 644

Query: 253 LSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 312
           LSGCTALRLLKLAPKLVK+GKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP
Sbjct: 645 LSGCTALRLLKLAPKLVKEGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEP 704

Query: 313 IGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           IGDG VGELTMALSDLLWDDMVSGPETERIPVSYT
Sbjct: 705 IGDGHVGELTMALSDLLWDDMVSGPETERIPVSYT 739

BLAST of HG10013677 vs. ExPASy TrEMBL
Match: A0A6J1CDV8 (D-amino-acid transaminase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010583 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 312/353 (88.39%), Postives = 328/353 (92.92%), Query Frame = 0

Query: 1   MEALNSPSNETE---AIG--NGTE--TDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMY 60
           MEA N  SNE+    AIG  NG+E  TDLKVHVFSSS ELLEKLHEKWSLVKKKPYPAMY
Sbjct: 1   MEAANPRSNESRGELAIGNENGSESGTDLKVHVFSSSSELLEKLHEKWSLVKKKPYPAMY 60

Query: 61  SSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKIS 120
           SSIFGGI TDPA+MVIPIDDHMVHRGHGVFDTA++LNGHLYELD HL+RFLRSASKAKIS
Sbjct: 61  SSIFGGITTDPALMVIPIDDHMVHRGHGVFDTAVLLNGHLYELDGHLNRFLRSASKAKIS 120

Query: 121 SPFPLSILRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQ 180
            PFP SILRSILIQL  ASQLKKGTLRYWLSAGPG+FLLTPAGNA S FYAVAIDEDFSQ
Sbjct: 121 PPFPWSILRSILIQLAVASQLKKGTLRYWLSAGPGNFLLTPAGNANSTFYAVAIDEDFSQ 180

Query: 181 CKEGVKTITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNV 240
           CKEGVK ITST+PMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNV
Sbjct: 181 CKEGVKVITSTIPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNV 240

Query: 241 NVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEM 300
           NVAFIT EKELILPSFDK+LSGCTALRLLKLAPKLV++GKLKSVGTAN+TVKEAKGAAEM
Sbjct: 241 NVAFITTEKELILPSFDKILSGCTALRLLKLAPKLVEEGKLKSVGTANVTVKEAKGAAEM 300

Query: 301 MFVGSTLPLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSY 347
           MFVGSTLP+LPII+WDEEPIGDG  GELTMALSDLLWDDMVSGP+TER+PV Y
Sbjct: 301 MFVGSTLPILPIITWDEEPIGDGRAGELTMALSDLLWDDMVSGPKTERVPVPY 353

BLAST of HG10013677 vs. ExPASy TrEMBL
Match: A0A6J1IJS3 (D-amino-acid transaminase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476905 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.7e-172
Identity = 309/347 (89.05%), Postives = 324/347 (93.37%), Query Frame = 0

Query: 1   MEALNSPSNETEAIGNGTETDLKVHVFSSSFELLEKLHEKWSLVKKKPYPAMYSSIFGGI 60
           M A NS SNET AIGNG ETDLKVHVFSSS EL EKLHEKWSL KKKPYPAMYSSIFGGI
Sbjct: 1   MAASNSHSNETGAIGNGCETDLKVHVFSSSLELHEKLHEKWSL-KKKPYPAMYSSIFGGI 60

Query: 61  ITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISSPFPLSI 120
           ITDPAMM+IPIDDHMVHRGHGVFDTAIILNGHLYELDAH+DRFLRSA+KAKISSPFP S+
Sbjct: 61  ITDPAMMMIPIDDHMVHRGHGVFDTAIILNGHLYELDAHIDRFLRSAAKAKISSPFPRSV 120

Query: 121 LRSILIQLTAASQLKKGTLRYWLSAGPGDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKT 180
           LR ILIQLTA S+LKKGTLRYWLSAGPGDFLLTP+G+  SAFYAVAID+DFSQCKEGVK 
Sbjct: 121 LRHILIQLTAVSELKKGTLRYWLSAGPGDFLLTPSGHGNSAFYAVAIDDDFSQCKEGVKA 180

Query: 181 ITSTVPMKTPQFATMKNVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN 240
           ITSTVP+KTPQFATMK+VNYLPNVL KMEAEEKGAFASIWVDEEGY+AEGPNVNVAFIT 
Sbjct: 181 ITSTVPIKTPQFATMKSVNYLPNVLTKMEAEEKGAFASIWVDEEGYVAEGPNVNVAFITK 240

Query: 241 EKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTL 300
           EKELILP FDKVLSGCTALRLL LAPKLV+DGKLKSVGTANLTV+EAK AAEMMFVGSTL
Sbjct: 241 EKELILPCFDKVLSGCTALRLLALAPKLVEDGKLKSVGTANLTVEEAKDAAEMMFVGSTL 300

Query: 301 PLLPIISWDEEPIGDGSVGELTMALSDLLWDDMVSGPETERIPVSYT 348
           P+LPIISWD+EPIGDG VGELTMALSDLLWDDMVSG E ERIPV YT
Sbjct: 301 PVLPIISWDDEPIGDGRVGELTMALSDLLWDDMVSGSEIERIPVPYT 346

BLAST of HG10013677 vs. TAIR 10
Match: AT5G57850.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 2.1e-98
Identity = 182/320 (56.88%), Postives = 236/320 (73.75%), Query Frame = 0

Query: 29  SSFELLEKLHEKWSLVK-KKPYPAMYSSIFGGIITDPAMMVIPIDDHMVHRGHGVFDTAI 88
           SS+E+ E+L     L +  + + AMYSS+  GI TDPA MV+P+DDHMVHRGHGVFDTA+
Sbjct: 58  SSYEVGERL----KLARGGQQFLAMYSSVVDGITTDPAAMVLPLDDHMVHRGHGVFDTAL 117

Query: 89  ILNGHLYELDAHLDRFLRSASKAKISSPFPLSILRSILIQLTAASQLKKGTLRYWLSAGP 148
           I+NG+LYELD HLDR LRSAS AKI  PF    ++ ILIQ  + S  + G+LRYWLSAGP
Sbjct: 118 IINGYLYELDQHLDRILRSASMAKIPLPFDRETIKRILIQTVSVSGCRDGSLRYWLSAGP 177

Query: 149 GDFLLTPAGNAKSAFYAVAIDEDFSQCKEGVKTITSTVPMKTPQFATMKNVNYLPNVLAK 208
           GDFLL+P+   K   YA+ I  +F+    GVK +TS++P+K P+FAT+K+VNYLPNVL++
Sbjct: 178 GDFLLSPSQCLKPTLYAIVIKTNFAINPIGVKVVTSSIPIKPPEFATVKSVNYLPNVLSQ 237

Query: 209 MEAEEKGAFASIWVDEEGYIAEGPNVNVAFITN-EKELILPSFDKVLSGCTALRLLKLAP 268
           MEAE KGA+A IWV ++G+IAEGPN+NVAF+ N  KEL++P FD VLSGCTA R L LA 
Sbjct: 238 MEAEAKGAYAGIWVCKDGFIAEGPNMNVAFVVNGGKELVMPRFDNVLSGCTAKRTLTLAE 297

Query: 269 KLVKDGKLKSVGTANLTVKEAKGAAEMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALS 328
           +LV  G LK+V   ++TV++ K A EMM +GS +P+ P+I WDEE IG+G  G +  AL 
Sbjct: 298 QLVSKGILKTVKVMDVTVEDGKKADEMMLIGSGIPIRPVIQWDEEFIGEGKEGPIAKALL 357

Query: 329 DLLWDDMVSGPETERIPVSY 347
           DLL +DM SGP + R+ V Y
Sbjct: 358 DLLLEDMRSGPPSVRVLVPY 373

BLAST of HG10013677 vs. TAIR 10
Match: AT3G05190.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.8e-13
Identity = 69/276 (25.00%), Postives = 122/276 (44.20%), Query Frame = 0

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISS-PF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ HLDR   SA      + P 
Sbjct: 267 GDEILPREMAKVSVFDSVVQGGDSVWEGLRIYKGKIFKLEEHLDRLFDSAKALAFDNVPA 326

Query: 118 PLSILRSILIQLTAASQLKKGTLRYWLSAGPG-DFLLTPAGNAKSAFYAVAID--EDFSQ 177
              +  +I   L          +R  L+ G      ++PA N       V  +       
Sbjct: 327 REEVKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAYNRYGCTLIVLAEWKPPVYD 386

Query: 178 CKEGVKTITSTVPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGP 237
            + G+  +T+T    +P     K  + N L N+LAK+E+    A  +I +D++GY++E  
Sbjct: 387 NEGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNTNAADAIMLDKDGYVSETN 446

Query: 238 NVNVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VK+  +  +    +++ E   A 
Sbjct: 447 ATNI-FMVKKGCVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAN 506

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALSD 328
           E+   G+   L P++  D   IGDG VG +T  L +
Sbjct: 507 EVWTTGTMGELSPVVKIDGRVIGDGKVGPVTRTLQN 536

BLAST of HG10013677 vs. TAIR 10
Match: AT5G27410.1 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-12
Identity = 67/276 (24.28%), Postives = 121/276 (43.84%), Query Frame = 0

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISS-PF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ HLDR   SA     ++ P 
Sbjct: 264 GDEIVPREMAKVSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT 323

Query: 118 PLSILRSILIQLTAASQLKKGTLRYWLSAGPG-DFLLTPAGNAKSAFYAVAID--EDFSQ 177
              I  +I   L          +R  L+ G      ++PA N       V  +       
Sbjct: 324 REEIKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYD 383

Query: 178 CKEGVKTITSTVPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGP 237
              G+  +T+T    +P     K  + N L N+LAK+E+       +I +D++G+++E  
Sbjct: 384 NDGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETN 443

Query: 238 NVNVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VK+  +  +    +++ E   A 
Sbjct: 444 ATNI-FMVKKDRVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAD 503

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALSD 328
           E+   G+   L P++  D   IG+G VG +T  L +
Sbjct: 504 EVWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRLQN 533

BLAST of HG10013677 vs. TAIR 10
Match: AT5G27410.2 (D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 5.2e-12
Identity = 67/276 (24.28%), Postives = 121/276 (43.84%), Query Frame = 0

Query: 58  GGIITDPAMMVIPIDDHMVHRGHGVFDTAIILNGHLYELDAHLDRFLRSASKAKISS-PF 117
           G  I    M  + + D +V  G  V++   I  G +++L+ HLDR   SA     ++ P 
Sbjct: 641 GDEIVPREMAKVSVFDSVVQGGDSVWEGLRIYKGKVFKLEEHLDRLSDSAKALAFNNVPT 700

Query: 118 PLSILRSILIQLTAASQLKKGTLRYWLSAGPG-DFLLTPAGNAKSAFYAVAID--EDFSQ 177
              I  +I   L          +R  L+ G      ++PA N       V  +       
Sbjct: 701 REEIKEAIFRTLITNGMFDNTHIRLSLTRGKKVTSGMSPAFNRYGCTLIVLAEWKPPVYD 760

Query: 178 CKEGVKTITSTVPMKTPQFATMK--NVNYLPNVLAKMEAEEKGAFASIWVDEEGYIAEGP 237
              G+  +T+T    +P     K  + N L N+LAK+E+       +I +D++G+++E  
Sbjct: 761 NDGGIVLVTATTRRNSPNNLDSKIHHNNLLNNILAKIESNNANVDDAIMLDKDGFVSETN 820

Query: 238 NVNVAFITNEKELILPSFDKVLSGCTALRLLKLAPKLVKDGKLKSVGTANLTVKEAKGAA 297
             N+ F+  +  ++ P  D  L G T   +++L   +VK+  +  +    +++ E   A 
Sbjct: 821 ATNI-FMVKKDRVLTPHADYCLPGITRATVMEL---VVKENFI--LEERRISLSEFHTAD 880

Query: 298 EMMFVGSTLPLLPIISWDEEPIGDGSVGELTMALSD 328
           E+   G+   L P++  D   IG+G VG +T  L +
Sbjct: 881 EVWTTGTMGELSPVVKIDGRVIGEGKVGPVTRRLQN 910

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900252.18.8e-18795.68D-amino-acid transaminase, chloroplastic-like isoform X2 [Benincasa hispida][more]
XP_004146044.14.2e-18193.08D-amino-acid transaminase, chloroplastic [Cucumis sativus] >KGN55100.1 hypotheti... [more]
XP_038900251.12.7e-18094.44D-amino-acid transaminase, chloroplastic-like isoform X1 [Benincasa hispida][more]
XP_008463704.11.4e-17992.51PREDICTED: D-amino-acid transaminase, chloroplastic-like [Cucumis melo][more]
KAA0066805.14.5e-17593.13hypothetical protein E6C27_scaffold271G001630 [Cucumis melo var. makuwa] >TYK279... [more]
Match NameE-valueIdentityDescription
Q8L4933.0e-9756.88D-amino-acid transaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAAT... [more]
Q584142.5e-1926.09Putative branched-chain-amino-acid aminotransferase OS=Methanocaldococcus jannas... [more]
O293292.1e-1826.15Putative branched-chain-amino-acid aminotransferase OS=Archaeoglobus fulgidus (s... [more]
O075976.4e-1525.87D-alanine aminotransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=dat PE... [more]
P635111.8e-1224.73D-alanine aminotransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) ... [more]
Match NameE-valueIdentityDescription
A0A0A0KZK62.0e-18193.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G629460 PE=4 SV=1[more]
A0A1S3CJV56.6e-18092.51D-amino-acid transaminase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A5D3DWQ32.2e-17593.13Aminotran_1_2 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1CDV86.0e-17388.39D-amino-acid transaminase, chloroplastic-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1IJS31.7e-17289.05D-amino-acid transaminase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G57850.12.1e-9856.88D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT3G05190.12.8e-1325.00D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT5G27410.15.2e-1224.28D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
AT5G27410.25.2e-1224.28D-aminoacid aminotransferase-like PLP-dependent enzymes superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043132Branched-chain-amino-acid aminotransferase-like, C-terminalGENE3D3.20.10.10coord: 169..338
e-value: 5.5E-35
score: 122.2
IPR043131Branched-chain-amino-acid aminotransferase-like, N-terminalGENE3D3.30.470.10coord: 20..167
e-value: 9.8E-23
score: 82.6
IPR001544Aminotransferase class IVPFAMPF01063Aminotran_4coord: 81..308
e-value: 7.1E-41
score: 140.5
NoneNo IPR availablePANTHERPTHR42743:SF8BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE-LIKEcoord: 7..345
NoneNo IPR availablePANTHERPTHR42743AMINO-ACID AMINOTRANSFERASEcoord: 7..345
IPR017824Aminodeoxychorismate lyase, class IVCDDcd01559ADCL_likecoord: 73..329
e-value: 1.67918E-73
score: 226.036
IPR036038Aminotransferase-like, PLP-dependent enzymesSUPERFAMILY56752D-aminoacid aminotransferase-like PLP-dependent enzymescoord: 57..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013677.1HG10013677.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0046656 folic acid biosynthetic process
molecular_function GO:0008696 4-amino-4-deoxychorismate lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity