HG10013582 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10013582 vs. NCBI nr
Match: KAG7024466.1 (AFG1-like ATPase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1039.6 bits (2687), Expect = 1.0e-299
Identity = 559/684 (81.73%), Postives = 573/684 (83.77%), Query Frame = 0

Query: 2   WLVSGKNTSSLSIFVAVFRYINDLELSIR--EIATFSSCRKTTRYTSNRRRRRNCDSKMK 61
           WLVSGKN S  SIFVAV  +        R  ++  F    +TTRYTS RRRRRNCDS+MK
Sbjct: 18  WLVSGKNKSLFSIFVAVCSFGASTIWRSRFWKLQRFLLV-ETTRYTSRRRRRRNCDSEMK 77

Query: 62  RGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSGLLNQYKHLVERGELQHDP 121
           RGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K  GLL+QYKHLVERGELQHDP
Sbjct: 78  RGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKSPGLLSQYKHLVERGELQHDP 137

Query: 122 FQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVWTS 181
           FQERVAS+LESLLGRLEQYEKDMEEYHVKLANWKQ+RENERRRLLMEEAESKQQGDVWTS
Sbjct: 138 FQERVASELESLLGRLEQYEKDMEEYHVKLANWKQSRENERRRLLMEEAESKQQGDVWTS 197

Query: 182 VNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGN 241
           VNKRRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGN
Sbjct: 198 VNKRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYGN 257

Query: 242 VGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINEDM 301
           VGSGKTMLMDMFYNATEG+VKHRQRYHFHE        IGIG+Q YSI+WQAMLKINEDM
Sbjct: 258 VGSGKTMLMDMFYNATEGIVKHRQRYHFHE--------IGIGIQVYSIIWQAMLKINEDM 317

Query: 302 HKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLVD 361
           HKIWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFLVD
Sbjct: 318 HKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFLVD 377

Query: 362 QQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQKD 421
           QQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQKD
Sbjct: 378 QQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQKD 437

Query: 422 IFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ------------------------- 481
           IFQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQ                         
Sbjct: 438 IFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEITSQ 497

Query: 482 ---------------------------------------VGAADYIALAKNYHTVFISDI 541
                                                  VGAADYIALAKNYHTVFISDI
Sbjct: 498 LGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISDI 557

Query: 542 PMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEA 601
           P+MSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEA
Sbjct: 558 PVMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEA 617

Query: 602 EGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLH 619
           EGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLH
Sbjct: 618 EGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNLH 677

BLAST of HG10013582 vs. NCBI nr
Match: KAG6591578.1 (AFG1-like ATPase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 995.3 bits (2572), Expect = 2.3e-286
Identity = 531/644 (82.45%), Postives = 542/644 (84.16%), Query Frame = 0

Query: 40  KTTRYTSNRRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKP 99
           +TTRYTS RRRRRNCDS+MKRGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K 
Sbjct: 42  ETTRYTSRRRRRRNCDSEMKRGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKS 101

Query: 100 SGLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENE 159
            GLL+QYKHLVERGELQHDPFQERVAS+LESLLGRLEQYEKD+EEYHVKLANWKQ+RENE
Sbjct: 102 PGLLSQYKHLVERGELQHDPFQERVASELESLLGRLEQYEKDLEEYHVKLANWKQSRENE 161

Query: 160 RRRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDS 219
           RRRLLMEEAESKQQGDVWTSVNKRRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDS
Sbjct: 162 RRRLLMEEAESKQQGDVWTSVNKRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDS 221

Query: 220 LVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIG 279
           LVGRCPSAP+APRGLYIYGNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE          
Sbjct: 222 LVGRCPSAPNAPRGLYIYGNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------- 281

Query: 280 IGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKY 339
                      AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKY
Sbjct: 282 -----------AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKY 341

Query: 340 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTV 399
           KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTV
Sbjct: 342 KQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTV 401

Query: 400 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----- 459
           LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQ     
Sbjct: 402 LVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFW 461

Query: 460 -----------------------------------------------------------V 519
                                                                      V
Sbjct: 462 PSDCTSVGKFEKMWVEITSQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPV 521

Query: 520 GAADYIALAKNYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 579
           GAADYIALAKNYHTVFISDIP+MSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLF
Sbjct: 522 GAADYIALAKNYHTVFISDIPVMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLF 581

Query: 580 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 619
           QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA
Sbjct: 582 QGTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRA 641

BLAST of HG10013582 vs. NCBI nr
Match: XP_038900047.1 (AFG1-like ATPase isoform X1 [Benincasa hispida] >XP_038900048.1 AFG1-like ATPase isoform X1 [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 1.6e-279
Identity = 518/626 (82.75%), Postives = 525/626 (83.87%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSGLLNQYKHLVERGELQH 117
           MKRGPSTSTISSLLSLRFTRQQSSKFG DP  VRFLSSSTKP GLLN+Y+ LVERGELQH
Sbjct: 1   MKRGPSTSTISSLLSLRFTRQQSSKFGADPLRVRFLSSSTKPPGLLNKYRLLVERGELQH 60

Query: 118 DPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVW 177
           DPFQERVAS+LESLLGRLEQYEKDMEEYHVKLANWKQNRE ERRRLLMEEAESKQQG+VW
Sbjct: 61  DPFQERVASELESLLGRLEQYEKDMEEYHVKLANWKQNREKERRRLLMEEAESKQQGNVW 120

Query: 178 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 237
           TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY
Sbjct: 121 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 180

Query: 238 GNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINE 297
           GNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE                     AMLKINE
Sbjct: 181 GNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------------------AMLKINE 240

Query: 298 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 357
           DMH IWKNQ+REKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL
Sbjct: 241 DMHNIWKNQMREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 300

Query: 358 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQ 417
           VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQ
Sbjct: 301 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQ 360

Query: 418 KDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----------------------- 477
           K+IFQ FV KLEEHCEFVLIGSEIDYRRFIAQRSFDQ                       
Sbjct: 361 KEIFQNFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDSTSVGKFENMWVEIT 420

Query: 478 -----------------------------------------VGAADYIALAKNYHTVFIS 537
                                                    VGAADYIALAKNYHTVFIS
Sbjct: 421 SQLGGQITSKTIPVMFGRKLEVSENCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS 480

Query: 538 DIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 597
           DIPMMSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET
Sbjct: 481 DIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 540

Query: 598 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 619
           EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN
Sbjct: 541 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 600

BLAST of HG10013582 vs. NCBI nr
Match: XP_022936535.1 (AFG1-like ATPase [Cucurbita moschata] >XP_022936536.1 AFG1-like ATPase [Cucurbita moschata])

HSP 1 Score: 969.9 bits (2506), Expect = 1.0e-278
Identity = 517/626 (82.59%), Postives = 524/626 (83.71%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSGLLNQYKHLVERGELQH 117
           MKRGPSTSTI SLLSL+FTRQQSSK GVDP  VRFLSSS K  GLL+QYKHLVERGELQH
Sbjct: 1   MKRGPSTSTIYSLLSLKFTRQQSSKLGVDPLRVRFLSSSAKSPGLLSQYKHLVERGELQH 60

Query: 118 DPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVW 177
           DPFQERVAS+LESLLGRLEQYEKDMEEYHVKLANWKQ+RENERRRLLMEEAESKQQGDVW
Sbjct: 61  DPFQERVASELESLLGRLEQYEKDMEEYHVKLANWKQSRENERRRLLMEEAESKQQGDVW 120

Query: 178 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 237
           TSVNKRRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY
Sbjct: 121 TSVNKRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 180

Query: 238 GNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINE 297
           GNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE                     AMLKINE
Sbjct: 181 GNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------------------AMLKINE 240

Query: 298 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 357
           DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFL
Sbjct: 241 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFL 300

Query: 358 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQ 417
           VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQ
Sbjct: 301 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQ 360

Query: 418 KDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----------------------- 477
           KDIFQKFVTKLEEHC FV IGSEIDYRRFIAQRSFDQ                       
Sbjct: 361 KDIFQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEIT 420

Query: 478 -----------------------------------------VGAADYIALAKNYHTVFIS 537
                                                    VGAADYIALAKNYHTVFIS
Sbjct: 421 SQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS 480

Query: 538 DIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 597
           DIPMMSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET
Sbjct: 481 DIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 540

Query: 598 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 619
           EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN
Sbjct: 541 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 600

BLAST of HG10013582 vs. NCBI nr
Match: XP_023536384.1 (AFG1-like ATPase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965.3 bits (2494), Expect = 2.5e-277
Identity = 515/626 (82.27%), Postives = 522/626 (83.39%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSGLLNQYKHLVERGELQH 117
           MKRGPSTSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K  GLL+QYKHLVERGELQH
Sbjct: 1   MKRGPSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKSPGLLSQYKHLVERGELQH 60

Query: 118 DPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVW 177
           DPFQERVAS+LESLLGRLEQYEKDMEEYHVKLANWKQ+RENERRRLLMEEAESKQQGDVW
Sbjct: 61  DPFQERVASELESLLGRLEQYEKDMEEYHVKLANWKQSRENERRRLLMEEAESKQQGDVW 120

Query: 178 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 237
           TSVN+RRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY
Sbjct: 121 TSVNRRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 180

Query: 238 GNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINE 297
           GNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE                     AMLKINE
Sbjct: 181 GNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------------------AMLKINE 240

Query: 298 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 357
           DMH IWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFL
Sbjct: 241 DMHNIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFL 300

Query: 358 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQ 417
           VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQ
Sbjct: 301 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQ 360

Query: 418 KDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----------------------- 477
           KDIFQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQ                       
Sbjct: 361 KDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEIT 420

Query: 478 -----------------------------------------VGAADYIALAKNYHTVFIS 537
                                                    VGAADYIALAKNYHTVFIS
Sbjct: 421 SQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS 480

Query: 538 DIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 597
           DIPMMSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET
Sbjct: 481 DIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 540

Query: 598 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 619
           EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN
Sbjct: 541 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 600

BLAST of HG10013582 vs. ExPASy Swiss-Prot
Match: Q8WV93 (AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 4.9e-34
Identity = 115/431 (26.68%), Postives = 182/431 (42.23%), Query Frame = 0

Query: 217 DSLVGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFR 276
           + L  +  S    PRGLY+YG+VG+GKTM+MDMFY   E  +K ++R HFH         
Sbjct: 116 EGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVE--MKRKKRVHFH--------- 175

Query: 277 IGIGLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEE 336
                         ML +++ +H++ K  + ++       S+    P   ++ E      
Sbjct: 176 ------------GFMLDVHKRIHRL-KQSLPKRKPGFMAKSYDPIAPIAEEISE------ 235

Query: 337 KYKQEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIG 396
                                        A +LCFDE Q  D+   + L  +   L   G
Sbjct: 236 ----------------------------EACLLCFDEFQVTDIADAMILKQLFENLFKNG 295

Query: 397 TVLVATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR------------ 456
            V+VATSNR P+DL ++G+Q+  F  F+  L+E+C  V + S IDYR+            
Sbjct: 296 VVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGIDYRKRELPAAGKLYYL 355

Query: 457 -------FIAQRSFDQ------------------------------------------VG 516
                   +  + FD+                                          +G
Sbjct: 356 TSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEELCERPLG 415

Query: 517 AADYIALAKNYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQ 576
           A+DY+ L+KN+ T+F+ +IP  ++  R + RRFITLID  Y+    + C A+T I  LF 
Sbjct: 416 ASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSLFL 472

Query: 577 GTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAV 587
                            +    +L ++ ++  ++G        +SM +G+EE+FAF+R +
Sbjct: 476 ----------------HQHHDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTI 472

BLAST of HG10013582 vs. ExPASy Swiss-Prot
Match: Q32PX9 (AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 6.4e-34
Identity = 120/426 (28.17%), Postives = 189/426 (44.37%), Query Frame = 0

Query: 230 PRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQ 289
           P+GLY+YG+VG+GKTM+MDMFY   E  VK ++R HFH                      
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYEYVE--VKSKRRVHFH---------------------G 188

Query: 290 AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEVQMKNIL 349
            ML +++ +H++ ++  + K            + F  K  + +A   E+  QE       
Sbjct: 189 FMLDVHKRIHRLKQSLPKRK------------VGFMAKSYDPIAPIAEEISQE------- 248

Query: 350 PAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPK 409
                            AS+LCFDE Q  D+   + L  +   L   G V+VATSNR P+
Sbjct: 249 -----------------ASLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPE 308

Query: 410 DLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR-------------------FIAQR 469
           DL ++G+Q+  F  F+  L+E+C  V + S +DYR+                    +  +
Sbjct: 309 DLYKNGLQRANFVPFIAVLKEYCNTVQLDSGVDYRKRELTPAGKLYYLTSEADVGTVMDK 368

Query: 470 SFDQ------------------------------------------VGAADYIALAKNYH 529
            FD+                                          +GA+DY+ L+KN+ 
Sbjct: 369 LFDELAQKQNDLTSPRILKVQGRELRLNKACGTVADCTFEELCERPLGASDYLELSKNFD 428

Query: 530 TVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLES 589
           TV I +IP  S+  R + RRFITLID  Y+    + C A+  I  LF    +        
Sbjct: 429 TVIIRNIPQFSLAKRTQVRRFITLIDNFYDFKVRIICSASVPISSLFVYQHQ-------- 478

Query: 590 FQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY- 593
              ++E++  ++  + L       +   +A +SM +G+EE+F+F+R +SRL EMQT  Y 
Sbjct: 489 ---DSESDQSRVLMDDL------GLSQDSAGLSMFTGEEEIFSFQRTLSRLTEMQTEQYW 478

BLAST of HG10013582 vs. ExPASy Swiss-Prot
Match: Q3V384 (AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.1e-33
Identity = 121/426 (28.40%), Postives = 188/426 (44.13%), Query Frame = 0

Query: 230 PRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQ 289
           P+GLY+YG+VG+GKTM+MDMFY   E   K ++R HFH                      
Sbjct: 129 PKGLYVYGDVGTGKTMVMDMFYAYVE--TKRKKRVHFH---------------------G 188

Query: 290 AMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLA-AEEKYKQEVQMKNIL 349
            ML ++  +H + ++  + K+             F  K  + +A   E+  QE       
Sbjct: 189 FMLDVHRRIHHLKQSLPKRKAG------------FMAKSYDPIAPIAEEISQE------- 248

Query: 350 PAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPK 409
                             S+LCFDE Q  D+   + L  +   L   G V+VATSNR P+
Sbjct: 249 -----------------TSLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPE 308

Query: 410 DLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR-------------------FIAQR 469
           DL ++G+Q+  F  F+  L+E+C+ + + S +DYR+                    +  +
Sbjct: 309 DLYKNGLQRANFVPFIAVLKEYCDTLQLDSGVDYRKRELAPAGKLYYLTSEADVEAVVDK 368

Query: 470 SFDQ------------------------------------------VGAADYIALAKNYH 529
            FD+                                          +GA+DY+ L+KN+ 
Sbjct: 369 LFDELAQKQNDLTSPRILKVQGRELRLNKACGSVADCTFEELCERPLGASDYLELSKNFD 428

Query: 530 TVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLES 589
           TV I +IP  S+  R +ARRFITLID  Y+    + C A+  I  LF          L  
Sbjct: 429 TVIIRNIPQFSLAKRTQARRFITLIDNFYDFKVRIICSASAPISSLF----------LHQ 478

Query: 590 FQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLY- 593
            Q ++E++  ++  + L       +   +A +SM +G+EE+FAF+R +SRL EMQT  Y 
Sbjct: 489 HQ-DSESDQSRILMDDL------GLSQDSAGLSMFTGEEEIFAFQRTISRLTEMQTEQYW 478

BLAST of HG10013582 vs. ExPASy Swiss-Prot
Match: P46441 (Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 4.3e-30
Identity = 117/422 (27.73%), Postives = 177/422 (41.94%), Query Frame = 0

Query: 229 APRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVW 288
           AP+G+YIYG+VG GKT LMDMFY+  + + K +QR HF+                     
Sbjct: 109 APKGMYIYGSVGGGKTTLMDMFYSCCDDIPK-KQRVHFN--------------------- 168

Query: 289 QAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFD--TKVKEWLAAEEKYKQEVQMKN 348
             M K++  +HK+     +E+  Q    +    LPFD    V E +A E           
Sbjct: 169 SFMSKVHGLIHKV----KQERGPQDRAFNSEKPLPFDPTLPVAEMIANE----------- 228

Query: 349 ILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRA 408
                              + ++CFDE Q  D+   + L  + + L + G V +ATSNR 
Sbjct: 229 -------------------SWLICFDEFQVTDIADAMILKSLFTHLFNEGIVCIATSNRH 288

Query: 409 PKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR---------FIA---------- 468
           P DL ++G+Q+  F  F+  L   C    + S +DYRR         F+           
Sbjct: 289 PNDLYKNGLQRSNFIPFIGVLLNRCNVAAMDSGVDYRRIAQSGDTNYFVTTQTDAKSQME 348

Query: 469 ----------------------------QRSFDQV-------------GAADYIALAKNY 528
                                       QR+  QV             G +DYI + + +
Sbjct: 349 RMFKILCSQENDIIRPRTITHFGRDLTFQRTCGQVLDSNFEELCNRPLGGSDYIQIGQFF 408

Query: 529 HTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLE 588
           HTV I D+P +++ ++ + RRFITLID LY++   +   A   ++ LF  T++    DL 
Sbjct: 409 HTVLIHDVPQLTLLLKSQMRRFITLIDTLYDNRVRVVISAEVPLDQLFSFTDKPK--DLA 458

BLAST of HG10013582 vs. ExPASy Swiss-Prot
Match: Q5TYS0 (Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 5.6e-30
Identity = 109/423 (25.77%), Postives = 179/423 (42.32%), Query Frame = 0

Query: 225 SAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFY 284
           S PH P+G YIYGNVG+GKTMLMD+FY+  E   + ++R HF+                 
Sbjct: 147 SKPHPPQGYYIYGNVGTGKTMLMDLFYSFVEN--RRKKRVHFN----------------- 206

Query: 285 SIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQM 344
                 ML ++  +HK+ ++  + +  + ++   I  +  +        AEE        
Sbjct: 207 ----GFMLDVHRRIHKLKQSLPKRRIGKMTMYDPIFPVAME-------IAEE-------- 266

Query: 345 KNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSN 404
                                  ++CFDE Q VD+   + L  +   L   G V+VATSN
Sbjct: 267 ---------------------TCLICFDEFQVVDIADAMILKQLFEGLFKCGVVVVATSN 326

Query: 405 RAPKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR-------------------- 464
           R P++L ++G+Q+  F  F+  L+E+C  V + + ID+R                     
Sbjct: 327 RPPEELYKNGLQRAAFVPFIGVLKEYCRIVSLDTGIDFRTREMKPAGRLYYISSEPDAEN 386

Query: 465 ------------------------------------FIAQRSFDQ-----VGAADYIALA 524
                                                IA  SF +     +GA DY+ +A
Sbjct: 387 AVNALFEELAFRQNDVTRPRVLNVQGREVTLSRTCGTIADCSFQELCEQPLGAGDYLEIA 446

Query: 525 KNYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALF 584
           + + TV I ++P + + ++D+ARRF TLID  Y+    +  LA   ++ L    ++G + 
Sbjct: 447 RCFDTVIIRNVPYLQLGMKDQARRFTTLIDNFYDQKVRVVMLADAPLDRLL---DQGQM- 494

Query: 585 DLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQT 587
                        G+  R+ L+   +G     +  +++ +  EE+FAF+R VSRL EMQT
Sbjct: 507 ------------TGEEARDRLMLDELGLTDEASKRITLFTADEEIFAFQRTVSRLAEMQT 494

BLAST of HG10013582 vs. ExPASy TrEMBL
Match: A0A0A0LHA9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G878760 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.8e-279
Identity = 531/682 (77.86%), Postives = 552/682 (80.94%), Query Frame = 0

Query: 1   MWLVSGKNTSSLSIFVAVFRYINDLELSIREIATFSSCRKTTRYTSNRRRRRNCDSKMKR 60
           M LVSGKNTSSLSIFV +F                    KT R+TS+RRRRRNCDS MKR
Sbjct: 1   MLLVSGKNTSSLSIFVLLF-------------------SKTIRHTSDRRRRRNCDSMMKR 60

Query: 61  GPSTSTISSLLSLRFTRQQ--SSKFGVDPC-VRFLSSSTKPSGLLNQYKHLVERGELQHD 120
           G STS+ISSLLSLRFTRQQ  SSKFGV+P  +RFLSS TKP GLL++Y+ LVERGELQHD
Sbjct: 61  GLSTSSISSLLSLRFTRQQSSSSKFGVNPLPLRFLSSFTKPPGLLDKYRVLVERGELQHD 120

Query: 121 PFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVWT 180
           PFQERVAS+LESLLGRLEQYEKDMEEYHVKLA WKQNRE ERRRLLMEEAESKQQGD   
Sbjct: 121 PFQERVASELESLLGRLEQYEKDMEEYHVKLAEWKQNREKERRRLLMEEAESKQQGD--- 180

Query: 181 SVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIYG 240
           +VN+RRSKL+ETLMFRK SENIEPGVGKWVSYLNRE+KLDSLVGRCPSAPHAPRGLYIYG
Sbjct: 181 AVNRRRSKLLETLMFRKKSENIEPGVGKWVSYLNREKKLDSLVGRCPSAPHAPRGLYIYG 240

Query: 241 NVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINED 300
           NVGSGKTMLMDMF+NATEG+VKHRQRYHFHE                     AMLKINED
Sbjct: 241 NVGSGKTMLMDMFFNATEGIVKHRQRYHFHE---------------------AMLKINED 300

Query: 301 MHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFLV 360
           MHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLA EEKYKQEVQMKNILPAVADKFLV
Sbjct: 301 MHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAGEEKYKQEVQMKNILPAVADKFLV 360

Query: 361 DQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQK 420
           DQQAG VGASILCFDEIQTVDVFAIVALSGI+SRLLS GTVLVATSNRAP DLNQDGMQK
Sbjct: 361 DQQAGHVGASILCFDEIQTVDVFAIVALSGIISRLLSTGTVLVATSNRAPNDLNQDGMQK 420

Query: 421 DIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ------------------------ 480
           DIFQKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQ                        
Sbjct: 421 DIFQKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSMRKFENMWVEITS 480

Query: 481 ----------------------------------------VGAADYIALAKNYHTVFISD 540
                                                   VGAADYIALAKNYHTVFIS+
Sbjct: 481 QLEGQITSETIRVMFGRKLEVPESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFISN 540

Query: 541 IPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETE 600
           IP+MSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETE
Sbjct: 541 IPIMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETE 600

Query: 601 AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNL 615
           AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN+
Sbjct: 601 AEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRNV 639

BLAST of HG10013582 vs. ExPASy TrEMBL
Match: A0A6J1FDZ4 (AFG1-like ATPase OS=Cucurbita moschata OX=3662 GN=LOC111443118 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.9e-279
Identity = 517/626 (82.59%), Postives = 524/626 (83.71%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSGLLNQYKHLVERGELQH 117
           MKRGPSTSTI SLLSL+FTRQQSSK GVDP  VRFLSSS K  GLL+QYKHLVERGELQH
Sbjct: 1   MKRGPSTSTIYSLLSLKFTRQQSSKLGVDPLRVRFLSSSAKSPGLLSQYKHLVERGELQH 60

Query: 118 DPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVW 177
           DPFQERVAS+LESLLGRLEQYEKDMEEYHVKLANWKQ+RENERRRLLMEEAESKQQGDVW
Sbjct: 61  DPFQERVASELESLLGRLEQYEKDMEEYHVKLANWKQSRENERRRLLMEEAESKQQGDVW 120

Query: 178 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 237
           TSVNKRRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY
Sbjct: 121 TSVNKRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 180

Query: 238 GNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINE 297
           GNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE                     AMLKINE
Sbjct: 181 GNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------------------AMLKINE 240

Query: 298 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 357
           DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFL
Sbjct: 241 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFL 300

Query: 358 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQ 417
           VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQ
Sbjct: 301 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQ 360

Query: 418 KDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----------------------- 477
           KDIFQKFVTKLEEHC FV IGSEIDYRRFIAQRSFDQ                       
Sbjct: 361 KDIFQKFVTKLEEHCAFVFIGSEIDYRRFIAQRSFDQVHYFWPSDCTSVGKFEKMWVEIT 420

Query: 478 -----------------------------------------VGAADYIALAKNYHTVFIS 537
                                                    VGAADYIALAKNYHTVFIS
Sbjct: 421 SQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS 480

Query: 538 DIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 597
           DIPMMSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET
Sbjct: 481 DIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 540

Query: 598 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 619
           EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN
Sbjct: 541 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 600

BLAST of HG10013582 vs. ExPASy TrEMBL
Match: A0A6J1IG43 (AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111476619 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.5e-277
Identity = 515/626 (82.27%), Postives = 521/626 (83.23%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQSSKFGVDP-CVRFLSSSTKPSGLLNQYKHLVERGELQH 117
           MKRG STSTI SLLSLRFTRQQSSK GVDP  VRFLSSS K  GLLNQYKHLVERGELQH
Sbjct: 1   MKRGTSTSTIYSLLSLRFTRQQSSKLGVDPLSVRFLSSSAKSPGLLNQYKHLVERGELQH 60

Query: 118 DPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGDVW 177
           DPFQERVAS+LESLLGRLEQYE+DMEEYHVKLANWKQNRENERRRLLMEEAESKQ GDVW
Sbjct: 61  DPFQERVASELESLLGRLEQYERDMEEYHVKLANWKQNRENERRRLLMEEAESKQPGDVW 120

Query: 178 TSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 237
           TSVNKRRSKL ETLMFRK+SENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY
Sbjct: 121 TSVNKRRSKLAETLMFRKSSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLYIY 180

Query: 238 GNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKINE 297
           GNVGSGKTMLMDMFYNATEG+VKHRQRYHFHE                     AMLKINE
Sbjct: 181 GNVGSGKTMLMDMFYNATEGIVKHRQRYHFHE---------------------AMLKINE 240

Query: 298 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADKFL 357
           DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEW+AAEEKYKQEVQMKNILPAVADKFL
Sbjct: 241 DMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWVAAEEKYKQEVQMKNILPAVADKFL 300

Query: 358 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDGMQ 417
           VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRAPKDLNQDGMQ
Sbjct: 301 VDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRAPKDLNQDGMQ 360

Query: 418 KDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ----------------------- 477
           KDIFQKFVTKLEEHC FVLIGSEIDYRRFIAQRSFDQ                       
Sbjct: 361 KDIFQKFVTKLEEHCAFVLIGSEIDYRRFIAQRSFDQMHYFWPSDCTSVGKFEKMWVEIT 420

Query: 478 -----------------------------------------VGAADYIALAKNYHTVFIS 537
                                                    VGAADYIALAKNYHTVFIS
Sbjct: 421 SQLGGQITSETIPVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVFIS 480

Query: 538 DIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 597
           DIP MSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET
Sbjct: 481 DIPTMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFET 540

Query: 598 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGVRN 619
           EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYL+GVRN
Sbjct: 541 EAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLKGVRN 600

BLAST of HG10013582 vs. ExPASy TrEMBL
Match: A0A6J1C362 (AFG1-like ATPase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007517 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 1.0e-271
Identity = 508/636 (79.87%), Postives = 524/636 (82.39%), Query Frame = 0

Query: 48  RRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDPC-VRFLSSSTKPSGLLNQYK 107
           RRRRRNC SKM+RGPSTSTISS LSLR  R QSS  G+D   VRFLSSS+KP GLL  Y+
Sbjct: 27  RRRRRNCVSKMRRGPSTSTISSFLSLRIAR-QSSNSGIDLIRVRFLSSSSKPPGLLVHYR 86

Query: 108 HLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEE 167
           HLVERGELQHDPFQERVAS+LESLLGRLEQYEKDME+YHVKLANWKQNRENERR+LLMEE
Sbjct: 87  HLVERGELQHDPFQERVASELESLLGRLEQYEKDMEDYHVKLANWKQNRENERRKLLMEE 146

Query: 168 AESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSA 227
           AESKQQGDVWTSVNKR+SKLVETLMFR  SENIEPGVGKWVSYLNRERKLDSLVGRCPSA
Sbjct: 147 AESKQQGDVWTSVNKRQSKLVETLMFRNKSENIEPGVGKWVSYLNRERKLDSLVGRCPSA 206

Query: 228 PHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSI 287
           PHAPRGLYIYGNVGSGKTMLMDMFYNAT+G+VKHR+RYHFHE                  
Sbjct: 207 PHAPRGLYIYGNVGSGKTMLMDMFYNATKGIVKHRRRYHFHE------------------ 266

Query: 288 VWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKN 347
              AMLKINEDMH+IWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKN
Sbjct: 267 ---AMLKINEDMHQIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKN 326

Query: 348 ILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRA 407
           ILPAVADKFLVDQQA QVGASILCFDEIQTVDVFAIVALSGIVSRLLS GTVLVATSNRA
Sbjct: 327 ILPAVADKFLVDQQADQVGASILCFDEIQTVDVFAIVALSGIVSRLLSTGTVLVATSNRA 386

Query: 408 PKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ------------- 467
           PKDLNQDGMQKDIFQKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQ             
Sbjct: 387 PKDLNQDGMQKDIFQKFVAKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLELTSVG 446

Query: 468 ---------------------------------------------------VGAADYIAL 527
                                                              VGAADYIAL
Sbjct: 447 KFEKMWAEMTRQLGGQITSETISVMFGRKLEVSESCNGVARFAFDFLCGQPVGAADYIAL 506

Query: 528 AKNYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGAL 587
           A+NYHTVFIS+IPMMSM IRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEG L
Sbjct: 507 AQNYHTVFISNIPMMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGTL 566

Query: 588 FDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQ 619
           FDLESFQFETEAEGGKLRRNVL EGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQ
Sbjct: 567 FDLESFQFETEAEGGKLRRNVLEEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQ 626

BLAST of HG10013582 vs. ExPASy TrEMBL
Match: A0A1S3CR63 (lactation elevated protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503816 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.5e-264
Identity = 495/629 (78.70%), Postives = 518/629 (82.35%), Query Frame = 0

Query: 58  MKRGPSTSTISSLLSLRFTRQQ--SSKFGVDPC-VRFLSSSTKPSGLLNQYKHLVERGEL 117
           MKRG STS+ISSLLSLRFTRQQ  SSKFGVDP  +RFLSS TKP GLL++Y+ LVERGEL
Sbjct: 1   MKRGLSTSSISSLLSLRFTRQQFSSSKFGVDPLRLRFLSSFTKPPGLLDKYRVLVERGEL 60

Query: 118 QHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENERRRLLMEEAESKQQGD 177
           QHDPFQERVAS+LESLLGRLEQYEKDME+YHVKLA W+QNRE ERRRLLMEEAESKQQGD
Sbjct: 61  QHDPFQERVASELESLLGRLEQYEKDMEDYHVKLAEWEQNREKERRRLLMEEAESKQQGD 120

Query: 178 VWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSLVGRCPSAPHAPRGLY 237
              +VNKRRSKL ETLMFRK +ENIEPGVGKWVSYLNRE+KLDSL+GRCP+APHAPRGLY
Sbjct: 121 ---AVNKRRSKLFETLMFRKKAENIEPGVGKWVSYLNREKKLDSLIGRCPAAPHAPRGLY 180

Query: 238 IYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQAMLKI 297
           IYGNVGSGKTMLMDMF+NAT+G+VKHRQRYHFHE                     AMLKI
Sbjct: 181 IYGNVGSGKTMLMDMFFNATQGIVKHRQRYHFHE---------------------AMLKI 240

Query: 298 NEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPAVADK 357
           NEDMHKIWKNQVREKSSQSS SSWIMNLPFDTKVKEWLA EEKYKQEVQMK+ILPAVADK
Sbjct: 241 NEDMHKIWKNQVREKSSQSSFSSWIMNLPFDTKVKEWLAGEEKYKQEVQMKHILPAVADK 300

Query: 358 FLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDLNQDG 417
           FL+DQQAG VGASILCFDEIQTVDVFAIVALSGI+SRLLS GTVLVATSNRAP DLNQDG
Sbjct: 301 FLIDQQAGHVGASILCFDEIQTVDVFAIVALSGIISRLLSTGTVLVATSNRAPNDLNQDG 360

Query: 418 MQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ--------------------- 477
           MQKDIFQKFV KLEEHCEFVLIGSEIDYRRFIAQRSFDQ                     
Sbjct: 361 MQKDIFQKFVIKLEEHCEFVLIGSEIDYRRFIAQRSFDQVHYFWPLDCTSVRKFENMWVE 420

Query: 478 -------------------------------------------VGAADYIALAKNYHTVF 537
                                                      VGAADYIALAKNYHTVF
Sbjct: 421 VTSQLGGQITSETVPVMFGRKLEVPESCNGVARFAFDFLCGQPVGAADYIALAKNYHTVF 480

Query: 538 ISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQF 597
           IS+IP+MSM IRDKARRFITLIDELYNHHCCLFCLAATSIE+LFQGTEEGALFDLESFQF
Sbjct: 481 ISNIPIMSMRIRDKARRFITLIDELYNHHCCLFCLAATSIEELFQGTEEGALFDLESFQF 540

Query: 598 ETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGV 619
           ETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGV
Sbjct: 541 ETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTPLYLEGV 600

BLAST of HG10013582 vs. TAIR 10
Match: AT2G25530.1 (AFG1-like ATPase family protein )

HSP 1 Score: 676.0 bits (1743), Expect = 2.8e-194
Identity = 360/626 (57.51%), Postives = 433/626 (69.17%), Query Frame = 0

Query: 43  RYTSNRRRRRNCDSKMKRGPSTSTISSLLSLRFTRQQSSKFGVDPCVRFLSSS---TKPS 102
           R  S +RR  N   K +  P+ S IS++L L F+ ++    G+    R  S+S    +PS
Sbjct: 51  RKASLQRRNHNRSLKQQIMPNVSQISTVLRLAFSCKRRLSNGLHFVARSYSTSDPPKQPS 110

Query: 103 GLLNQYKHLVERGELQHDPFQERVASKLESLLGRLEQYEKDMEEYHVKLANWKQNRENER 162
           G L  Y  LVE+G LQHDP+QE+V S  E+L GRLE +EK ME+YHV+LA W++ RE ER
Sbjct: 111 GPLTNYSKLVEQGRLQHDPYQEKVVSAFENLFGRLEHFEKQMEDYHVRLAEWEKKREEER 170

Query: 163 RRLLMEEAESKQQGDVWTSVNKRRSKLVETLMFRKNSENIEPGVGKWVSYLNRERKLDSL 222
           R+L++EEAE K+   +W SVNK+  KL+   +  +   N+EPGVGKWVSYLNRERKLDS+
Sbjct: 171 RKLMVEEAEKKEDDGMWASVNKQGQKLLGRWVLGRRQMNVEPGVGKWVSYLNRERKLDSI 230

Query: 223 VGRCPSAPHAPRGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGI 282
           VG  P+ P AP+GLYIYGNVG GKTMLMDMFY AT+G+++HRQR+HFHE           
Sbjct: 231 VGSRPAVPPAPKGLYIYGNVGCGKTMLMDMFYGATDGIIRHRQRFHFHE----------- 290

Query: 283 GLQFYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYK 342
                     AMLKINE MHK WK    EK  Q SISSWIMNLP D KVKEWLA EE YK
Sbjct: 291 ----------AMLKINEQMHKYWKENGAEKPMQYSISSWIMNLPVDEKVKEWLAGEEFYK 350

Query: 343 QEVQMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVL 402
           Q++QMK+ILPAVADKFLVDQQ+ + GASILCFDEIQTVDVFAIVALSGI+SRLL+ GTVL
Sbjct: 351 QQLQMKHILPAVADKFLVDQQSSKKGASILCFDEIQTVDVFAIVALSGIMSRLLTTGTVL 410

Query: 403 VATSNRAPKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFIAQRSFDQ------ 462
           VATSNRAP++LNQDGMQK+IF KF++KLE+HCE + IGSE+DYRR  A+ S +       
Sbjct: 411 VATSNRAPRELNQDGMQKEIFDKFISKLEKHCEIISIGSEVDYRRVAAKNSAENVHYLWP 470

Query: 463 ----------------------------------------------------------VG 522
                                                                     VG
Sbjct: 471 LNDAVLEEFEKMWRQVTDQYGGEITSATLPVMFGRTVEVPESCSGVARFTFEYLCGRPVG 530

Query: 523 AADYIALAKNYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQ 582
           AADYIA+AKNYHT+FISDIP MSM IRDKARRFITL+DELYNHHCCL   A T+I++LFQ
Sbjct: 531 AADYIAVAKNYHTIFISDIPAMSMEIRDKARRFITLVDELYNHHCCLVSSAETAIDELFQ 590

Query: 583 GTEEGALFDLESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAV 602
           GT EG LFDLESFQFETE E  +LRR+VL EG++ + G+P++IVSMLSG+EEMFAF RA 
Sbjct: 591 GTAEGTLFDLESFQFETETEDSRLRRDVLAEGSISAAGSPSSIVSMLSGEEEMFAFARAA 650

BLAST of HG10013582 vs. TAIR 10
Match: AT4G30490.1 (AFG1-like ATPase family protein )

HSP 1 Score: 111.7 bits (278), Expect = 2.1e-24
Identity = 104/424 (24.53%), Postives = 161/424 (37.97%), Query Frame = 0

Query: 224 PSAPHAP-RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQ 283
           P   ++P +GLY+YG VG+GKTMLMD+F++      K +QR HFH+              
Sbjct: 154 PQTSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWK-KQRIHFHD-------------- 213

Query: 284 FYSIVWQAMLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEV 343
                   ML ++  + K                                          
Sbjct: 214 -------FMLSVHSRLQK----------------------------------------HK 273

Query: 344 QMKNILPAVADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVAT 403
            + + L  VA +   D       A +LC DE    DV   + L+ +   L S G +LVAT
Sbjct: 274 GLSDPLEVVAQEIAHD-------AILLCLDEFMVTDVADALILNRLFGHLFSNGVILVAT 333

Query: 404 SNRAPKDLNQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRF----------------I 463
           SNR P  L + G+Q+D+F  F++ L+E      IGS +DYR+                 +
Sbjct: 334 SNRNPDKLYEGGLQRDLFLPFISSLKERSVVHEIGSAVDYRKLTSAEQGFYFIGKDLSTL 393

Query: 464 AQRSFDQ------------------------------------------VGAADYIALAK 523
            ++ F Q                                          +GAADY  L K
Sbjct: 394 LKQKFRQLIGDNVVARPQVVEVVMGRKLQIPLGANGCAYFPFEELCDRPLGAADYFGLFK 453

Query: 524 NYHTVFISDIPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFD 583
            +HT+ + +IP+  +H R  A RF+TL+D +Y +   L C A  + ++L +      +  
Sbjct: 454 KFHTLALDEIPVFGLHNRTAAYRFVTLVDVMYENRARLLCTAEANPQELLE-----KIIT 489

Query: 584 LESFQFETEAEGGKLRRNVLVEGNVGSVGAPTAIVSMLSGQEEMFAFRRAVSRLIEMQTP 589
           +   +        + R+N + E              +    E  FA  R +SRL EM + 
Sbjct: 514 ISEAKSMGPRTSSRSRKNDVTE--------------LCVDNELGFAKDRTISRLTEMNSK 489

BLAST of HG10013582 vs. TAIR 10
Match: AT4G28070.2 (AFG1-like ATPase family protein )

HSP 1 Score: 104.4 bits (259), Expect = 3.4e-22
Identity = 103/416 (24.76%), Postives = 160/416 (38.46%), Query Frame = 0

Query: 231 RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQA 290
           +GLY+YG VG+GKTMLMD+F++      +  QR HFH                       
Sbjct: 134 KGLYLYGGVGTGKTMLMDLFFHQLPASWR-TQRIHFH---------------------NF 193

Query: 291 MLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPA 350
           ML ++  + K        K  +  +   ++ L                            
Sbjct: 194 MLSVHSRLQK-------HKGLEDPLE--VVGL---------------------------E 253

Query: 351 VADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDL 410
           +AD+           A +LC DE    DV   + L+ +   L + G +LVATSNRAP +L
Sbjct: 254 IADE-----------AILLCLDEFMVNDVADALILNRLFRHLFNNGIILVATSNRAPDNL 313

Query: 411 NQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRR-------------------------- 470
            + G+Q+D+F  F++ L+E C    IGS +DYR+                          
Sbjct: 314 YEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIGKDISGLLKQKFQLL 373

Query: 471 ------------------------------FIAQRSFDQ-VGAADYIALAKNYHTVFISD 530
                                         F+ +   D+ +GAADY+ L K +HT+ +  
Sbjct: 374 VGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLGLFKKFHTLALEG 433

Query: 531 IPMMSMHIRDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETE 589
           +P   +H R  A RF+TL+D +Y +   L C A  S  +L +      + D +     T 
Sbjct: 434 VPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIV--TISDAQQIAPRTS 460

BLAST of HG10013582 vs. TAIR 10
Match: AT4G28070.1 (AFG1-like ATPase family protein )

HSP 1 Score: 89.7 bits (221), Expect = 8.7e-18
Identity = 99/408 (24.26%), Postives = 152/408 (37.25%), Query Frame = 0

Query: 231 RGLYIYGNVGSGKTMLMDMFYNATEGVVKHRQRYHFHERCMPFVFRIGIGLQFYSIVWQA 290
           +GLY+YG VG+GKTMLMD+F++      +  QR HFH                       
Sbjct: 134 KGLYLYGGVGTGKTMLMDLFFHQLPASWR-TQRIHFH---------------------NF 193

Query: 291 MLKINEDMHKIWKNQVREKSSQSSISSWIMNLPFDTKVKEWLAAEEKYKQEVQMKNILPA 350
           ML ++  + K        K  +  +   ++ L                            
Sbjct: 194 MLSVHSRLQK-------HKGLEDPLE--VVGL---------------------------E 253

Query: 351 VADKFLVDQQAGQVGASILCFDEIQTVDVFAIVALSGIVSRLLSIGTVLVATSNRAPKDL 410
           +AD+           A +LC DE    DV   + L+ +   L + G +LVATSNRAP +L
Sbjct: 254 IADE-----------AILLCLDEFMVNDVADALILNRLFRHLFNNGIILVATSNRAPDNL 313

Query: 411 NQDGMQKDIFQKFVTKLEEHCEFVLIGSEIDYRRFI-AQRSFDQVGAADYIALAKNYHTV 470
            + G+Q+D+F  F++ L+E C    IGS +DYR+   A+  F  +G  D   L K    +
Sbjct: 314 YEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIG-KDISGLLKQKFQL 373

Query: 471 FISD------------------------------------------------IPMMSMHI 530
            + D                                                +P   +H 
Sbjct: 374 LVGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLGLFSVPFFGLHN 433

Query: 531 RDKARRFITLIDELYNHHCCLFCLAATSIEDLFQGTEEGALFDLESFQFETEAEGGKL-R 589
           R  A RF+TL+D +Y +   L C A  S  +L +      + D +     T +   K   
Sbjct: 434 RTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIV--TISDAQQIAPRTSSRSRKSDD 451