HG10013548 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013548
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAspartate--tRNA ligase
LocationChr02: 2486462 .. 2489454 (-)
RNA-Seq ExpressionHG10013548
SyntenyHG10013548
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCGCTTCATCCGAAACTCCGATTGATGAGCCGCCCTCCGCCGATGACGAATCTTCCAAAGCCGTCAGTAAGAAGGCGGCCAAGAAGGAGGCTGCCAAGCAGGAAAAGCTCCGCCGTCGCCAAGAAGCCGCTGCTGAAGCTGCTATTTCGTCCCTCAATGTCGAGGAAGACCCGCTTTCCGCTAACTATGGCGATGTACCACTATCCGATCTCCAATCCAAGGAGGCTAAGGATATTAAAAACTGGACTCAGGTCGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTGCGGGGTCGCGTTCAGACGATCCGTGCTGTTGCCAAGAAAATGGCGTTTTTGGTGGTGAGAGAAAGGGGATTCACCGTACAATGCGTCGTGTCCGAGCAGCCGGAGCTCGTTAGTCGCCAGATGGTGAAGTATGTCTGTGGATTGAGCCGTGAGTCCATCGTCGACGTTGAAGGAGTCGTGTCCGTGCCTAATGTTGCCATTAAAGGTGCTACGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTCTGCCTATAAATATTGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCGTTGCAGGTATACTTTGTAATCGATCTCTTTTGATGTAATCTTCTTCGTGATTGTCTAATGTCTGTTGTGTAGTTGTTCATGTTCTAATGATCTTCTTCTTTTTCATCATCGTCATTTTAAAGAAAGATTTGTAGACCTCCTGTTTAACGTCAGACTAATTTGTTGCCCGCAGGCTGGTGAGCAACTAGTACGTGTCAATCAGGATACGCGGTTGAACTACAGAGTCCTTGACATGCGAACTCCAGCTAACCAAGGAATTTTTCGCATTCAATCTAAAATTTCTGATGTGAGTTCTCTTTGTTATTTTATGTTTTTTCTGATTCTTCTGTTTGTTGATCCAAATCTATGGTTGTATTGCATGAAATTGAAGTGTACGGGTTAATTTGAAGTTGATCATTTTAGCTATAGCTATCATTTTCAGCCTGCTCGGTTTGGTTTCCGTTGTGTTGTTCATTGGCCATCTTTAACTTGTGAGGAAGAATAGATTTTTTTTGTGGGGTTGTAGGTATGGGGAGGATCTTTGGGATTTGATTAGATTAAAATCCTCTCAGTGGGTGTATCTTAATAGAGTTTTTCGCAATTATCGGCTTAGCTTTATTCTTTTGGAATGGGACCCCTCGTGTAATTGGGTGCGAGGGTTTTGTTTTGGTTTTTGTTTTTTGAATTGAATGCCCTTGTTTATTCTTTCATCATCTTCTGTGAAAACATGGTTGCTTCTCATGTTAAAAGAAACATGTGACTATATTCTCATTATGCAGATATTTAGACAATTTTTGTTGGGTGAAGACTTTGTTGAAATCCACACTCCAAAAACGATTAAAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAAGTTTGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGCGTATTTGAGATTGGCTCCGTTTTTAGAGCAGAGGACTCATACACACACAGACATTTATGTGAATTTGTAGGACTTGATGTTGAAATGGAGATCAAGGCTCATTACTCTGAGGTGATGCTCTAAGTGGGTGGCTTTTCATTTGTCGTGGCCATATTTATTTGATATTCAATCCGACTTTTTTTCTTCTGTTTTAGTGCAGGTGATGGACGTTGTGGACCGATTATTCGTCTCAATTTTTGACAGTTTGAATCAAATCTGTAAGGCGGAGCTTGAAGCAGTTAATAGACAGTATCCCTTTGAACCTTTGAAGGTGAACTTTATTGATTTCTTACTTCGTAAAAATCTTCATAGCACGGACCAAAGTGTTAGACATTCTTTTGGTTATGGATTTGTGCCAATAGATATGTACAATGTGGATGGATATATGACTGAGTTTGGATAGTTGAATGATTTCCTCTCATTTCCATCTTTGATTCGAGTTCATGAATATGCAGTACTTGCGAAACACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGTGAGTGGTTTGATTCTTTTTCCCTCACAAATCATCAAAAGAGATCCCATGTCCCAAATGAGATGACATTTTCTTCTTTGTTATGTATGATTATCTGCATAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACAACTCGTCTTAGAGAAGTAATTCGAGTTCTTGCTTATTTAGTTATGAACTTATGTTTTTGTTCAATTAGTATATCTGAAGATTGCTGATCAAACTTTTCTAACTAAAGATATGGTACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTATACCATGCCTTGCTATGATAACCCAACTTACAGCAACTCATTTGATGTCTTTATTCGAGGTATACATTATATATTACATCACTGCTTATTCTTCCCTAGAATTTGGGGTGTGTATTCATTTAGAACAAAATTTAATAATTAATGCTCCATGGTAAGTAACTTAAAACTTTGTTGTCTGCAGGGGAAGAAATAATTTCTGGGGCTCAGCGTGTCCACGTGCCAGAATTTCTAGCTGAACGTGCTCAAGCATATGGCATTGAATTAAAAACCATAGAAACCTATATTGATTCGTTCAGGTACATTTCTCTTTGTTTTCTCCTTTACTCAAAGTTGGAATTTAACCGACTAAGCGAGATTCGATTTGATTCAATTGTGATGTCGACGTTTTGATTAGGTTTCTCTTCACATAAATGCTTTCATTGCAGATATGGTGCACCTCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTGGTGATGCTTTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAG

mRNA sequence

ATGTCCGCTTCATCCGAAACTCCGATTGATGAGCCGCCCTCCGCCGATGACGAATCTTCCAAAGCCGTCAGTAAGAAGGCGGCCAAGAAGGAGGCTGCCAAGCAGGAAAAGCTCCGCCGTCGCCAAGAAGCCGCTGCTGAAGCTGCTATTTCGTCCCTCAATGTCGAGGAAGACCCGCTTTCCGCTAACTATGGCGATGTACCACTATCCGATCTCCAATCCAAGGAGGCTAAGGATATTAAAAACTGGACTCAGGTCGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTGCGGGGTCGCGTTCAGACGATCCGTGCTGTTGCCAAGAAAATGGCGTTTTTGGTGGTGAGAGAAAGGGGATTCACCGTACAATGCGTCGTGTCCGAGCAGCCGGAGCTCGTTAGTCGCCAGATGGTGAAGTATGTCTGTGGATTGAGCCGTGAGTCCATCGTCGACGTTGAAGGAGTCGTGTCCGTGCCTAATGTTGCCATTAAAGGTGCTACGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTCTGCCTATAAATATTGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCGTTGCAGGCTGGTGAGCAACTAGTACGTGTCAATCAGGATACGCGGTTGAACTACAGAGTCCTTGACATGCGAACTCCAGCTAACCAAGGAATTTTTCGCATTCAATCTAAAATTTCTGATATATTTAGACAATTTTTGTTGGGTGAAGACTTTGTTGAAATCCACACTCCAAAAACGATTAAAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAAGTTTGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGCGTATTTGAGATTGGCTCCGTTTTTAGAGCAGAGGACTCATACACACACAGACATTTATGTGAATTTGTAGGACTTGATGTTGAAATGGAGATCAAGGCTCATTACTCTGAGGTGATGGACGTTGTGGACCGATTATTCGTCTCAATTTTTGACAGTTTGAATCAAATCTGTAAGGCGGAGCTTGAAGCAGTTAATAGACAGTATCCCTTTGAACCTTTGAAGTACTTGCGAAACACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACAACTCGTCTTAGAGAAATATGGTACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTATACCATGCCTTGCTATGATAACCCAACTTACAGCAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATAATTTCTGGGGCTCAGCGTGTCCACGTGCCAGAATTTCTAGCTGAACGTGCTCAAGCATATGGCATTGAATTAAAAACCATAGAAACCTATATTGATTCGTTCAGATATGGTGCACCTCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTGGTGATGCTTTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAG

Coding sequence (CDS)

ATGTCCGCTTCATCCGAAACTCCGATTGATGAGCCGCCCTCCGCCGATGACGAATCTTCCAAAGCCGTCAGTAAGAAGGCGGCCAAGAAGGAGGCTGCCAAGCAGGAAAAGCTCCGCCGTCGCCAAGAAGCCGCTGCTGAAGCTGCTATTTCGTCCCTCAATGTCGAGGAAGACCCGCTTTCCGCTAACTATGGCGATGTACCACTATCCGATCTCCAATCCAAGGAGGCTAAGGATATTAAAAACTGGACTCAGGTCGGCTCTTTGACGGGGGAGCTTAAAGACAAGTATGTGATTCTGCGGGGTCGCGTTCAGACGATCCGTGCTGTTGCCAAGAAAATGGCGTTTTTGGTGGTGAGAGAAAGGGGATTCACCGTACAATGCGTCGTGTCCGAGCAGCCGGAGCTCGTTAGTCGCCAGATGGTGAAGTATGTCTGTGGATTGAGCCGTGAGTCCATCGTCGACGTTGAAGGAGTCGTGTCCGTGCCTAATGTTGCCATTAAAGGTGCTACGCAACAGGTGGAAATTCAAGTGAGGAAAGTATATTGCATCAGTAAGGCAATGCCGACTCTGCCTATAAATATTGAAGATGCTGCCCGTAGTGAAGCTGAAATTGAGAAGGCGTTGCAGGCTGGTGAGCAACTAGTACGTGTCAATCAGGATACGCGGTTGAACTACAGAGTCCTTGACATGCGAACTCCAGCTAACCAAGGAATTTTTCGCATTCAATCTAAAATTTCTGATATATTTAGACAATTTTTGTTGGGTGAAGACTTTGTTGAAATCCACACTCCAAAAACGATTAAAGGTTCTAGTGAAGGTGGTGCTTCTGTTTTTAAGTTTGATTACAAAGGCCAACCCGCTTGCCTGGCTCAGTCACCTCAGTTACATAAGCAAATGGCAATCTGTGGTGACTTTGGGCGCGTATTTGAGATTGGCTCCGTTTTTAGAGCAGAGGACTCATACACACACAGACATTTATGTGAATTTGTAGGACTTGATGTTGAAATGGAGATCAAGGCTCATTACTCTGAGGTGATGGACGTTGTGGACCGATTATTCGTCTCAATTTTTGACAGTTTGAATCAAATCTGTAAGGCGGAGCTTGAAGCAGTTAATAGACAGTATCCCTTTGAACCTTTGAAGTACTTGCGAAACACTCTACGACTTACATTTGAGGAAGGAATTCAGATGTTGAAGGATGCTGGCGTCGAAATTGATCCTCTTGGGGATCTTAATACAGAGGCTGAGAGGAAATTGGGACAACTCGTCTTAGAGAAATATGGTACGGAGTTCTACATTCTTCACCGCTATCCTTTGGCTGTTAGGCCATTCTATACCATGCCTTGCTATGATAACCCAACTTACAGCAACTCATTTGATGTCTTTATTCGAGGGGAAGAAATAATTTCTGGGGCTCAGCGTGTCCACGTGCCAGAATTTCTAGCTGAACGTGCTCAAGCATATGGCATTGAATTAAAAACCATAGAAACCTATATTGATTCGTTCAGATATGGTGCACCTCCACACGGTGGATTCGGAGTAGGTTTGGAGCGTGTGGTGATGCTTTTTTGTGGTTTGAATAACATTCGCAAAACATCATTGTTCCCTCGTGATCCACAGAGGATTGCTCCTTAG

Protein sequence

MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Homology
BLAST of HG10013548 vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 995.0 bits (2571), Expect = 2.6e-286
Identity = 504/545 (92.48%), Postives = 525/545 (96.33%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDEP SA DE+SKAVSKKAAKKEAAK EKLRRRQEA AEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELKDKYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           AICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HYSEVMD+VDRLFVS+FDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. NCBI nr
Match: XP_004136326.1 (aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical protein Csa_002506 [Cucumis sativus])

HSP 1 Score: 994.6 bits (2570), Expect = 3.4e-286
Identity = 504/545 (92.48%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASS+TPIDEPPSADD+SSKAVSKKAAKKEAAKQEKLRRRQEAAAE+AISSLNVE+DPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKE K I+NWTQVGSLT ELKDKYVILRGRVQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++S +FRQFLL E+FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           AICGDFGRVFEIG VFRAEDSYTHRHLCEF GLDVEMEIK HYSEVMD+VDRLFV++FDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LN+ CK  LEAV++QYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. NCBI nr
Match: KAG6591612.1 (Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 993.8 bits (2568), Expect = 5.8e-286
Identity = 503/545 (92.29%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDEP SA DE+SKAVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELK+KYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKEKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           +ICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HYSEVMD+VDRLFVS+FDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 993.8 bits (2568), Expect = 5.8e-286
Identity = 503/545 (92.29%), Postives = 525/545 (96.33%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDEP SA DE+SKAVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELKDKYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           +ICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HYSEVMD+VDRLFVS+FDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. NCBI nr
Match: XP_022976148.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 989.9 bits (2558), Expect = 8.4e-285
Identity = 502/545 (92.11%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDE  SA DE+SKAVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELKDKYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           +ICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HY EVMD+VDRLFVS+FDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. ExPASy Swiss-Prot
Match: Q9M084 (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 8.6e-208
Identity = 377/558 (67.56%), Postives = 443/558 (79.39%), Query Frame = 0

Query: 1   MSASSE-TPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE  P+    +A +ES +  SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKDIKNWTQVGSLTGELKDKYVILRGRVQTIR 120
           D  S NYGDV L++LQS          EA + K WT V  L  E+ +  V++RGRV T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVVRERGFTVQCVVSEQPEL-VSRQMVKYVCGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQP 300
           VLD+RTPANQ IF++Q ++   FR+ L  ++FV IHTPK + GSSEGG++VF+ +YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+G VFRAEDS+THRHLCEFVGLDVEMEI+ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEID 420
           D+VD LFV IF SLN+ CK EL+AV +QYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA   GI++KTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

Query: 541 NNIRKTSLFPRDPQRIAP 546
           NNIRKTSLFPRDPQR++P
Sbjct: 541 NNIRKTSLFPRDPQRLSP 558

BLAST of HG10013548 vs. ExPASy Swiss-Prot
Match: Q8H104 (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 6.2e-190
Identity = 344/531 (64.78%), Postives = 417/531 (78.53%), Query Frame = 0

Query: 17  DESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEE--DPLSANYGDVPLSDLQS 76
           +E  + +SKK +KK AAK EKL R+QE   EA  SSL++EE  +  S+NYGDV  ++LQS
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQE-REEATSSSLSLEEEDESCSSNYGDVTTNELQS 67

Query: 77  KEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQP 136
             A + K  T V +L  E+    V +RGR+   R V  K+ F+++RE GFTVQCVV E  
Sbjct: 68  --AVEGKELTDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFL 256
           +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQ ++   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGS 316
             + F+EIHTPK I GSSEGG++VF+ DYKGQPACLAQSPQLHKQMAICGD  RVFE+G 
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNR 376
           VFRAEDS+THRHLCEFVGLDVEMEI+ HYSE+MD+V  LF  IF  + + C  ELE+V +
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L  TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+  GI++K
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of HG10013548 vs. ExPASy Swiss-Prot
Match: P14868 (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2)

HSP 1 Score: 485.7 bits (1249), Expect = 6.7e-136
Identity = 263/508 (51.77%), Postives = 344/508 (67.72%), Query Frame = 0

Query: 39  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYV 98
           R+ QE   E     ++  ED     YG   +S +   + K  +   +V  LT +  D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVRDLTIQKADEVV 67

Query: 99  ILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEG 158
            +R RV T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 159 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 218
           VV   N  I   TQQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 219 VNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGAS 278
           VNQDTRL+ RV+D+RT  +Q +FR+QS I  +FR+ L+ + FVEI TPK I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 279 VFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEM 338
           VF   Y    A LAQSPQL+KQM IC DF +VF IG VFRAEDS THRHL EFVGLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 339 EIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQ 398
               HY EVM+ +    V IF  L +  + E++ VN+Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 399 MLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYS 458
           ML++AGVE+    DL+T  E+ LG LV EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 459 NSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGL 518
           NS+D+F+RGEEI+SGAQR+H P+ L ERA  +GI+L+ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 519 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of HG10013548 vs. ExPASy Swiss-Prot
Match: Q5R9I5 (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 8.8e-136
Identity = 262/508 (51.57%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 39  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYV 98
           R+ QE   E     ++  ED     YG   +S +   + K  +   +V  LT +  D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKSDRVLVRVRDLTIQKADEVV 67

Query: 99  ILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEG 158
            +R RV T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 159 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 218
           VV   N  I+  TQQ VE+ V+K+Y IS A P LP+ ++DA R EAE E+     E    
Sbjct: 128 VVRKVNQKIESCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 219 VNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGAS 278
           VNQDTRL+ RV+D+RT  +Q +FR+QS I  +FR+ L+ + FVEI TPK I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 279 VFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEM 338
           VF   Y    A LAQSPQL+KQM IC DF +VF IG VFRAEDS THRHL EFVGLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 339 EIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQ 398
               HY EVM+ +    V IF  L +  + E++ VN+Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 399 MLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYS 458
           ML++AGVE+    DL+T  E+ LG L+ EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLIKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 459 NSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGL 518
           NS+D+F+RGEEI+SGAQR+H P+ L ERA  +GI+L+ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 519 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of HG10013548 vs. ExPASy Swiss-Prot
Match: Q922B2 (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 4.8e-134
Identity = 260/508 (51.18%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 39  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYV 98
           R+ QE   E     ++  ED     YG   +S +   + K  +   +V  LT +  D  V
Sbjct: 8   RKGQEKPREI----VDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVKDLTVQKADDVV 67

Query: 99  ILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEG 158
            +R RV T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESI+DVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIIDVEG 127

Query: 159 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVR 218
           VV   N  I   TQQ VE+ V+K+Y IS A P LP+ ++DA R E E E+  +A      
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAIRPEVEGEEDGRA-----T 187

Query: 219 VNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGAS 278
           VNQDTRL+ RV+D+RT  +Q IFR+QS I  +FR+ L+ + FVEI TPK I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 279 VFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEM 338
           VF   Y    A LAQSPQL+KQM IC DF +VF IG VFRAEDS THRHL EFVGLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 339 EIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQ 398
               HY EV++ +    V IF  L +  + E++ V++Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 399 MLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYS 458
           ML++AGVE+D   DL+T  E+ LG+LV EKY T+FY+L +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQS 427

Query: 459 NSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGL 518
           NS+D+F+RGEEI+SGAQR+H P+ L ERA  +GI+L+ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 519 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of HG10013548 vs. ExPASy TrEMBL
Match: A0A0A0LDS7 (Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 504/545 (92.48%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASS+TPIDEPPSADD+SSKAVSKKAAKKEAAKQEKLRRRQEAAAE+AISSLNVE+DPL
Sbjct: 1   MSASSQTPIDEPPSADDDSSKAVSKKAAKKEAAKQEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKE K I+NWTQVGSLT ELKDKYVILRGRVQTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRVQTIRAVGKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEI+KALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIDKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++S +FRQFLL E+FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDENFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           AICGDFGRVFEIG VFRAEDSYTHRHLCEF GLDVEMEIK HYSEVMD+VDRLFV++FDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LN+ CK  LEAV++QYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNEKCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. ExPASy TrEMBL
Match: A0A6J1FFA5 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 2.8e-286
Identity = 503/545 (92.29%), Postives = 525/545 (96.33%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDEP SA DE+SKAVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELKDKYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           +ICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HYSEVMD+VDRLFVS+FDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. ExPASy TrEMBL
Match: A0A6J1ILA3 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.1e-285
Identity = 502/545 (92.11%), Postives = 524/545 (96.15%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASSETPIDE  SA DE+SKAVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKEAKD+ NWTQVGSLTGELKDKYVILRGR+QTIRAVAKKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCVVSEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMP+LPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++  IFRQFLL E FVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           +ICGDFGRVFEIG VFRAEDS+THRHLCEF GLDVEMEIK HY EVMD+VDRLFVS+FDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           +NQ CK ELEA+++QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. ExPASy TrEMBL
Match: A0A5A7TBL8 (Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006980 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.9e-285
Identity = 503/545 (92.29%), Postives = 523/545 (95.96%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASS+TPIDEP SA DESSKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVE+DPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKE K I+NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++S +FRQFLL EDFVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           AICGDFGRVFEIG VFRAEDSYTHRHLCEF GLDVEMEIK HYSEVMD+VDRLFV++FDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LN+ CK  LEAV++QYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. ExPASy TrEMBL
Match: A0A1S3CR09 (Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.9e-285
Identity = 503/545 (92.29%), Postives = 523/545 (95.96%), Query Frame = 0

Query: 1   MSASSETPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSASS+TPIDEP SA DESSKAVSKKAAKKEAAK EKLRRRQEAAAE+AISSLNVE+DPL
Sbjct: 1   MSASSQTPIDEPLSAGDESSKAVSKKAAKKEAAKLEKLRRRQEAAAESAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVR 120
           SANYGDVPLSDLQSKE K I+NWTQVGSLT ELKDKYVILRGR+QTIRAV KKMAFLVVR
Sbjct: 61  SANYGDVPLSDLQSKEVKSIENWTQVGSLTPELKDKYVILRGRIQTIRAVGKKMAFLVVR 120

Query: 121 ERGFTVQCVVSEQPELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           E+GFTVQCV+SEQPELVSRQMVKYV GLSRESIVDVEGVVSVPNVAIKGA+QQVEIQVRK
Sbjct: 121 EKGFTVQCVLSEQPELVSRQMVKYVDGLSRESIVDVEGVVSVPNVAIKGASQQVEIQVRK 180

Query: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240
           VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF
Sbjct: 181 VYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQM 300
           RIQ ++S +FRQFLL EDFVEIHTPK I GSSEGGASVF+ DYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVSTMFRQFLLDEDFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDS 360
           AICGDFGRVFEIG VFRAEDSYTHRHLCEF GLDVEMEIK HYSEVMD+VDRLFV++FDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVAMFDS 360

Query: 361 LNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420
           LN+ CK  LEAV++QYPFEPLKYLR TLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL
Sbjct: 361 LNERCKKALEAVDKQYPFEPLKYLRKTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQA GI++KTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

Query: 541 QRIAP 546
           QRIAP
Sbjct: 541 QRIAP 545

BLAST of HG10013548 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 724.5 bits (1869), Expect = 6.1e-209
Identity = 377/558 (67.56%), Postives = 443/558 (79.39%), Query Frame = 0

Query: 1   MSASSE-TPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE  P+    +A +ES +  SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKDIKNWTQVGSLTGELKDKYVILRGRVQTIR 120
           D  S NYGDV L++LQS          EA + K WT V  L  E+ +  V++RGRV T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVVRERGFTVQCVVSEQPEL-VSRQMVKYVCGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQP 300
           VLD+RTPANQ IF++Q ++   FR+ L  ++FV IHTPK + GSSEGG++VF+ +YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+G VFRAEDS+THRHLCEFVGLDVEMEI+ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEID 420
           D+VD LFV IF SLN+ CK EL+AV +QYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA   GI++KTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

Query: 541 NNIRKTSLFPRDPQRIAP 546
           NNIRKTSLFPRDPQR++P
Sbjct: 541 NNIRKTSLFPRDPQRLSP 558

BLAST of HG10013548 vs. TAIR 10
Match: AT4G31180.2 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 724.5 bits (1869), Expect = 6.1e-209
Identity = 377/558 (67.56%), Postives = 443/558 (79.39%), Query Frame = 0

Query: 1   MSASSE-TPIDEPPSADDESSKAVSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE  P+    +A +ES +  SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKDIKNWTQVGSLTGELKDKYVILRGRVQTIR 120
           D  S NYGDV L++LQS          EA + K WT V  L  E+ +  V++RGRV T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVVRERGFTVQCVVSEQPEL-VSRQMVKYVCGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSEA+IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDMRTPANQGIFRIQSKISDIFRQFLLGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQP 300
           VLD+RTPANQ IF++Q ++   FR+ L  ++FV IHTPK + GSSEGG++VF+ +YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+G VFRAEDS+THRHLCEFVGLDVEMEI+ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEID 420
           D+VD LFV IF SLN+ CK EL+AV +QYPFEPLK+L  TLRLTFEEG+QMLK+AGVE+D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA   GI++KTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

Query: 541 NNIRKTSLFPRDPQRIAP 546
           NNIRKTSLFPRDPQR++P
Sbjct: 541 NNIRKTSLFPRDPQRLSP 558

BLAST of HG10013548 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 665.2 bits (1715), Expect = 4.4e-191
Identity = 344/531 (64.78%), Postives = 417/531 (78.53%), Query Frame = 0

Query: 17  DESSKAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEE--DPLSANYGDVPLSDLQS 76
           +E  + +SKK +KK AAK EKL R+QE   EA  SSL++EE  +  S+NYGDV  ++LQS
Sbjct: 8   EECGEKISKKESKKRAAKLEKLLRKQE-REEATSSSLSLEEEDESCSSNYGDVTTNELQS 67

Query: 77  KEAKDIKNWTQVGSLTGELKDKYVILRGRVQTIRAVAKKMAFLVVRERGFTVQCVVSEQP 136
             A + K  T V +L  E+    V +RGR+   R V  K+ F+++RE GFTVQCVV E  
Sbjct: 68  --AVEGKELTDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEE-- 127

Query: 137 ELVSRQMVKYVCGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPTLPIN 196
             V   M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P LP+ 
Sbjct: 128 TRVGANMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLV 187

Query: 197 IEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFL 256
           +EDAARSE++IEK+ + G+Q  RV QDTRLN RVLD+RTPANQ IFRIQ ++   FR++L
Sbjct: 188 VEDAARSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL 247

Query: 257 LGEDFVEIHTPKTIKGSSEGGASVFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGS 316
             + F+EIHTPK I GSSEGG++VF+ DYKGQPACLAQSPQLHKQMAICGD  RVFE+G 
Sbjct: 248 QSKGFLEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGP 307

Query: 317 VFRAEDSYTHRHLCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNR 376
           VFRAEDS+THRHLCEFVGLDVEMEI+ HYSE+MD+V  LF  IF  + + C  ELE+V +
Sbjct: 308 VFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRK 367

Query: 377 QYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVLEKYGTEFYI 436
           QYPF+ LK+L  TLRLTF EGIQMLK+AG E+DPLGDLNTE+ERKLGQLVLEKY TEFY+
Sbjct: 368 QYPFQSLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYM 427

Query: 437 LHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELK 496
           LHRYP AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+  GI++K
Sbjct: 428 LHRYPSAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVK 487

Query: 497 TIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 546
           TI TYID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 488 TISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of HG10013548 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 9.0e-27
Identity = 85/272 (31.25%), Postives = 143/272 (52.57%), Query Frame = 0

Query: 282 DYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRHLCEFVGLDVEMEIKA 341
           D+ G+ A L  S QL  +   C     V+  G  FRAE+S+T RHL EF  ++ E+   A
Sbjct: 303 DFFGRQAFLTVSGQLQVETYACA-LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF-A 362

Query: 342 HYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYP---FEPLKYLRNTL--RLTFEEGI 401
              + M+  +     + + L + C A++E + + +     + LK + +T   R+T+ + I
Sbjct: 363 DLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAI 422

Query: 402 QMLKDA---GVEID---PLG-DLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMP 461
           ++L++A   G E D     G DL +E ER L +++ +K      I++ YP  ++ FY M 
Sbjct: 423 ELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKP----LIVYNYPKGIKAFY-MR 482

Query: 462 CYDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQAYGIELKTIETYIDSFRYGAP 521
             D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RYG  
Sbjct: 483 LNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTV 542

Query: 522 PHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 541
            H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 543 KHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567

BLAST of HG10013548 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 3.8e-25
Identity = 117/463 (25.27%), Postives = 198/463 (42.76%), Query Frame = 0

Query: 100 LRGRVQTIRAVAKKMAFLVVRERG--FTVQCVVSEQPELVSR-QMVKYVCGLSRESIVDV 159
           + G V+T+R+    + F+ + +      +QCV++   E   + +    + G S    V V
Sbjct: 114 IMGWVRTLRS-QSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGAS----VSV 173

Query: 160 EGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPTLPINIEDAARSEAEIEKALQAGEQLV 219
           +G +    VA +G  Q+VE++V K+  + +   + PI  +  +R     +  L+      
Sbjct: 174 QGTI----VASQGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTF 233

Query: 220 RVNQDTRLNYRVLDMRTPANQGIFRIQSKISDIFRQFLLGEDF-VEIHTPKTIKGS---- 279
                 R        +     G   + S I         GE F V    P + + +    
Sbjct: 234 GAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSI 293

Query: 280 -----SEGGASVFKFDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGSVFRAEDSYTHRH 339
                ++GG   +  D+ G+PA L  S QL+ +         V+  G  FRAE+S T RH
Sbjct: 294 DAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNGE-TYATALSDVYTFGPTFRAENSNTSRH 353

Query: 340 LCEFVGLDVEMEIKAHYSEVMDVVDRLFVSIFDSLNQICKAELEAVNRQYPFEPLKYL-- 399
           L EF  ++ E+   A   + M         +   +   CK ++E  +       ++ L  
Sbjct: 354 LAEFWMIEPELAF-ADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSD 413

Query: 400 ---RNTLRLTFEEGIQMLKDAGVEID-PLG---DLNTEAERKLGQLVLEKYGTEFYILHR 459
              +  L+L + + I++L  A  + D P+    DL +E ER + +   E +G    I+  
Sbjct: 414 VAEKEFLQLGYTDAIEILLKANKKFDFPVKWGLDLQSEHERYITE---EAFGGRPVIIRD 473

Query: 460 YPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQAYGIELKTIE 519
           YP  ++ FY     D  T +    +  R  E+I G+QR    E L  R     +  ++  
Sbjct: 474 YPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYW 533

Query: 520 TYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 541
            Y+D  RYG+ PH GFG+G ER+V    G++NIR    FPR P
Sbjct: 534 WYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPRTP 562

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023535979.12.6e-28692.48aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
XP_004136326.13.4e-28692.48aspartate--tRNA ligase 2, cytoplasmic [Cucumis sativus] >KGN60145.1 hypothetical... [more]
KAG6591612.15.8e-28692.29Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022936995.15.8e-28692.29aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata][more]
XP_022976148.18.4e-28592.11aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9M0848.6e-20867.56Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE... [more]
Q8H1046.2e-19064.78Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g268... [more]
P148686.7e-13651.77Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2[more]
Q5R9I58.8e-13651.57Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1[more]
Q922B24.8e-13451.18Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LDS71.6e-28692.48Aspartyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G881580 PE=3 SV=1[more]
A0A6J1FFA52.8e-28692.29Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1[more]
A0A6J1ILA34.1e-28592.11Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1[more]
A0A5A7TBL86.9e-28592.29Aspartyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CR096.9e-28592.29Aspartyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503781 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31180.16.1e-20967.56Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G31180.26.1e-20967.56Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G26870.14.4e-19164.78Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.19.0e-2731.25Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.13.8e-2525.27Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 25..47
NoneNo IPR availableGENE3D2.40.50.140coord: 56..190
e-value: 5.6E-39
score: 134.7
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 192..545
e-value: 2.9E-126
score: 423.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 14..40
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availablePANTHERPTHR43450:SF1ASPARTATE--TRNA LIGASE, CYTOPLASMICcoord: 15..545
NoneNo IPR availableCDDcd04320AspRS_cyto_Ncoord: 97..197
e-value: 3.15794E-43
score: 147.325
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 217..541
e-value: 2.8805E-159
score: 454.717
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 215..544
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 307..320
score: 48.21
coord: 290..302
score: 55.24
coord: 500..514
score: 55.15
coord: 460..476
score: 40.11
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 220..539
e-value: 1.2E-74
score: 251.3
IPR004523Aspartate-tRNA synthetase, type 2TIGRFAMTIGR00458TIGR00458coord: 92..545
e-value: 3.6E-138
score: 459.1
IPR004523Aspartate-tRNA synthetase, type 2PANTHERPTHR43450ASPARTYL-TRNA SYNTHETASEcoord: 15..545
IPR004523Aspartate-tRNA synthetase, type 2HAMAPMF_02075Asp_tRNA_synth_type2coord: 81..545
score: 39.121883
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 240..537
score: 17.854904
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 62..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013548.1HG10013548.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006422 aspartyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005829 cytosol
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004815 aspartate-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding