HG10013539 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10013539
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Ribosomal RNA large subunit methyltransferase H
Location	Chr02: 2414362 .. 2416780 (+)
RNA-Seq Expression	HG10013539
Synteny	HG10013539

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAATGGACAGAGAGCGGTGCCAATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTACAGCTTCTCGTTGATGAGTATATTCAAAAGCTCAACAACTACTGTCATGTTGAAGATGTTCAACTGCGGTCAAATCCAAGGAATGCGCGGTAGGTGATTGTATGGTTGATGTTAGTTTGTAAATTTTGTTTAATTTCATTACAATATCGGCTGAATGATGCTAGTCTTAATTAAATACTCTCATTCTGCGTATTGATACTTGAATTTTAGGGATCCGAGAGCTCAGGTTGATGATGAAGACATGGCTGTCGTGAATCTTATCAAGTCTGACGATTGGGTTTGTCTCTCAACATTCATTTTCGTCGGATCATTACATATTTCGCTATTCTGGCTGTGCTCGTTTACTCTAACTAGTTTGGAACTCTCTCTCTTTCTCAATTACATCTTTCTTTTGTCTGCTAAAAATGTGAAAGAAAATTGTATTATGGAACCTTTTTACTCTAGAAAGATGGGTCGTAGTGGTTATTTTCCTGGGACGAGATATAGGTTGCAATGTTTTTTGTTTGTGTTTTTTTTAAATTCAACCAATGTGGGGTAGGGGGATTTGAACCTCCAACCTCAAAAGGAGGAGTACGTGCCAATTATAGCATAACTTGTTGCTCTGTTTAGCCCTGACAAAAAAATGATCTGTAGAATGTTTTATAGTGCTGCTGCTACTGCTTACTATCTCCCTTCTGCATATGCACCTGCAGGTTGTGATGTTGGATGAGAATGGAAAAGAGGTATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGTGCGTACCATTTCCAATTCAAATCAAATTGGTTGTTTCATTATTATCAGTTCATCGCTACTAGACAGTATTCGTGATACTTAAATTTTCAAAGTTGGTTGTTATGGGCTCCTACGGAGTTTGGACTGAAAATTGAATGTTTTGATTATTCACAGTTTAACCAGCCCACATGTTCCACTTCCATTTCAGTCTAGATCTTAGCATGTAACAGTCGCTTGCTTTCTAATTTTATATGATGGAATTGCACAGATGGCTAGAAGTAGAACAAACAATATGTGGATTATTTTAGGTTGTCTTAGCCTCGTAAAGGTTTCCAATTATCACTGTGCTCCAAATGTTTCTGAAAACAACTTTAATAGAGCTATGGGGGTATGTTCAGAATGGACATGTCATTTTGTAGATGTTCTTTGATAACGTACTGTTTCCTTATGTTTCTTCTTCTGTGCAGGGTGCTTCAAGATTATCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACTTATCGATCAAGCTCTCTTCCATGGTTTTAAATCATCAAATTGCATTACTGGTGCTTATGGAACAACTTTACAGGTAACTTTATTTTTCTTCTAATCAATAGTGCCTTTTTCAGTTTTATTTCATTTGTATTGTATGTGACACGACATCAAACGCTTCAGAAGCAGAGTTACCTAAAGATCATATCTTGGGTAGAATATCCGTGTCTTAAGGGCTAGATGTTTTGTTGGTTAAACTGATCTGTTGATGATAATGAATTCAAGAGGTTGGACATACAATTCTATATTATCCCATCACACTCAGTTTTGTTCACTTCAGCCAAAATCTCTCTCCCTCCATGTGCCCTGCACCTAGAGTCTACTCAAACCTCATAGTTTATGGCTGCAAAATATTTTGAAATTTAATTTCCGAACATTAAATTTGTAATTTAAGCAACATTTTAAGACTTTTGAAATCAAACCGCTACTTTCTTTCACAAATTGACTTCAAAATCCATCCCAAAACACACAAAATTCACACTGCGGCGTCTTGCATTTCGAAGTTTACTGCATCAATGGTATACTAAAAGTATAAAATACTTGTTCTCAATGCATATGTATTATTTTTAGGTCATGGACTATTCTAAAAGGTCAGAAGTACCATCATTAGCAGCAGCAGCAGCTCATTTGCCAGCATAGCGGTATTTTGCCTTGATATGTTACATCTTTAGTTATCAAAGCTTGGTACAGCAGCGATGAACCTATTTCCTATCTGTTTCATCTTCACAATAGAAGGAGAAGTTAATTTTGTATAGAAATGATCTCTTATTGCCATTATTGCAGGAAATTTTGTTGCTTATTTTGTCCAAAGCATTTCCCTTCTGATCTTTCGTGTACTAGTATAAATTTGCCTAATTACTCTTGTAAGTATGTAAGTTTTAATGGGTCAAAAATTTGTCGGAGATTACTCTTGAGTTGTGCTAACATTGACATTACTCTTGTAAATTTTAATGTAGGGAATACTTGTTTTATACCATATTTGCTCGTGTGTTCATGATGAATGGAGCTCGAGGTGGAGAGTAA

mRNA sequence

ATGGACAATGGACAGAGAGCGGTGCCAATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTACAGCTTCTCGTTGATGAGTATATTCAAAAGCTCAACAACTACTGTCATGTTGAAGATGTTCAACTGCGGTCAAATCCAAGGAATGCGCGGGATCCGAGAGCTCAGGTTGATGATGAAGACATGGCTGTCGTGAATCTTATCAAGTCTGACGATTGGGTTGTGATGTTGGATGAGAATGGAAAAGAGGTATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAATGGCTAGAAGTAGAACAAACAATATGTGGATTATTTTAGGTTGTCTTAGCCTCGTAAAGGTTTCCAATTATCACTGTGCTCCAAATGTTTCTGAAAACAACTTTAATAGAGCTATGGGGGGTGCTTCAAGATTATCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACTTATCGATCAAGCTCTCTTCCATGGTTTTAAATCATCAAATTGCATTACTGGTGCTTATGGAACAACTTTACAGGGAATACTTGTTTTATACCATATTTGCTCGTGTGTTCATGATGAATGGAGCTCGAGGTGGAGAGTAA

Coding sequence (CDS)

ATGGACAATGGACAGAGAGCGGTGCCAATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTACAGCTTCTCGTTGATGAGTATATTCAAAAGCTCAACAACTACTGTCATGTTGAAGATGTTCAACTGCGGTCAAATCCAAGGAATGCGCGGGATCCGAGAGCTCAGGTTGATGATGAAGACATGGCTGTCGTGAATCTTATCAAGTCTGACGATTGGGTTGTGATGTTGGATGAGAATGGAAAAGAGGTATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAATGGCTAGAAGTAGAACAAACAATATGTGGATTATTTTAGGTTGTCTTAGCCTCGTAAAGGTTTCCAATTATCACTGTGCTCCAAATGTTTCTGAAAACAACTTTAATAGAGCTATGGGGGGTGCTTCAAGATTATCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACTTATCGATCAAGCTCTCTTCCATGGTTTTAAATCATCAAATTGCATTACTGGTGCTTATGGAACAACTTTACAGGGAATACTTGTTTTATACCATATTTGCTCGTGTGTTCATGATGAATGGAGCTCGAGGTGGAGAGTAA

Protein sequence

MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMAVVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNYHCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLMEQLYREYLFYTIFARVFMMNGARGGE

Homology

[Close]

BLAST of HG10013539 vs. NCBI nr
Match: XP_004136319.1 (putative RNA methyltransferase At5g10620 isoform X2 [Cucumis sativus] >KAE8651373.1 hypothetical protein Csa_002160 [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 1.3e-71
Identity = 150/187 (80.21%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLLVDEYI KL NYCHVEDVQLRSNPRNARDP+AQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQVDDEDVA 85

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+K DDWVVMLDENGKE+SSE MAELVADAGNT                    VS+ 
Sbjct: 86  VVNLLKPDDWVVMLDENGKELSSELMAELVADAGNT--------------------VSSC 145

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
           H AP VS NNFNRA+G ASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM
Sbjct: 146 HHAPIVSGNNFNRAIGSASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 192

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 206 EQLYRSW 192

[Close]

BLAST of HG10013539 vs. NCBI nr
Match: XP_031739112.1 (putative RNA methyltransferase At5g10620 isoform X1 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 6.3e-71
Identity = 150/188 (79.79%), Postives = 157/188 (83.51%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLLVDEYI KL NYCHVEDVQLRSNPRNARDP+AQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQVDDEDVA 85

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+K DDWVVMLDENGKE+SSE MAELVADAGNT                    VS+ 
Sbjct: 86  VVNLLKPDDWVVMLDENGKELSSELMAELVADAGNT--------------------VSSC 145

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
           H AP VS NNFNRA+G ASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM
Sbjct: 146 HHAPIVSGNNFNRAIGSASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 193

Query: 186 EQLYREYL 194
           EQLY + L
Sbjct: 206 EQLYSQDL 193

[Close]

BLAST of HG10013539 vs. NCBI nr
Match: XP_038896820.1 (putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida])

HSP 1 Score: 267.7 bits (683), Expect = 8.5e-68
Identity = 149/211 (70.62%), Postives = 157/211 (74.41%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVD 60
           MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYI+KL NYCH+EDV LRSNPRNARDPRAQVD
Sbjct: 24  MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIEKLKNYCHIEDVLLRSNPRNARDPRAQVD 83

Query: 61  DEDMAVVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLV 120
           DEDMAVVNL+KSDDWVVMLDENGKE+SSEQMA+LVADAG T                   
Sbjct: 84  DEDMAVVNLLKSDDWVVMLDENGKEMSSEQMADLVADAGTT------------------- 143

Query: 121 KVSNYHCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA 180
                                GASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA
Sbjct: 144 ---------------------GASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA 193

Query: 181 LLVLMEQLYREYLFYTIFARVFMMNGARGGE 212
           LLVLMEQLYR  + Y I +R+      RGGE
Sbjct: 204 LLVLMEQLYRVLVLYHICSRI-RDEWTRGGE 193

[Close]

BLAST of HG10013539 vs. NCBI nr
Match: XP_038896822.1 (putative RNA methyltransferase At5g10620 isoform X3 [Benincasa hispida])

HSP 1 Score: 261.9 bits (668), Expect = 4.7e-66
Identity = 142/192 (73.96%), Postives = 148/192 (77.08%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVD 60
           MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYI+KL NYCH+EDV LRSNPRNARDPRAQVD
Sbjct: 24  MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIEKLKNYCHIEDVLLRSNPRNARDPRAQVD 83

Query: 61  DEDMAVVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLV 120
           DEDMAVVNL+KSDDWVVMLDENGKE+SSEQMA+LVADAG T                   
Sbjct: 84  DEDMAVVNLLKSDDWVVMLDENGKEMSSEQMADLVADAGTT------------------- 143

Query: 121 KVSNYHCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA 180
                                GASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA
Sbjct: 144 ---------------------GASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA 175

Query: 181 LLVLMEQLYREY 193
           LLVLMEQLYR +
Sbjct: 204 LLVLMEQLYRSW 175

[Close]

BLAST of HG10013539 vs. NCBI nr
Match: KAG7037551.1 (putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 261.5 bits (667), Expect = 6.1e-66
Identity = 142/193 (73.58%), Postives = 150/193 (77.72%), Query Frame = 0


Query: 1   MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVD 60
           MDN QRAVPIRVLTVGKKRSRGVQLL+DEYI KLNNYC+VEDVQLRSNPRNARDPRAQVD
Sbjct: 1   MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD 60

Query: 61  DEDMAVVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLV 120
           DED+AVVNL+KSDDWVVMLDENGKE+SSEQMAELVADAGNT                   
Sbjct: 61  DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNT------------------- 120

Query: 121 KVSNYHCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIA 180
                                GASRL+FCIGGPYGHGQQLRQRAN+SIKLSSMVLNHQIA
Sbjct: 121 ---------------------GASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIA 153

Query: 181 LLVLMEQLYREYL 194
           LLVLMEQLYR +L
Sbjct: 181 LLVLMEQLYRGFL 153

[Close]

BLAST of HG10013539 vs. ExPASy Swiss-Prot
Match: Q9LXB4 (Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g10620 PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.8e-48
Identity = 105/187 (56.15%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RA+PIRV+TVGKKRS GV+LLVDEY  KL  YC  ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           ++ LI SDDWVV+LDE G+++ SEQMAEL+ DAGN+                        
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNS------------------------ 156

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASR+SFCIGG YGHG Q+R+RAN++I+LSSMVLNHQIAL+VLM
Sbjct: 157 ----------------GASRISFCIGGAYGHGTQVRKRANVTIRLSSMVLNHQIALVVLM 183

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 217 EQLYRSW 183

[Close]

BLAST of HG10013539 vs. ExPASy Swiss-Prot
Match: A0PXE8 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT) OX=386415 GN=rlmH PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 52/187 (27.81%), Postives = 86/187 (45.99%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQL--RSNPRNA--RDPRAQVDDEDMA 69
           I ++ VGK + + ++L +DEY ++L+ YC +  V+L     P NA  +D       E   
Sbjct: 3   ITIICVGKLKEKYLKLAIDEYSKRLSRYCKLNIVELNDEKTPDNASEKDELIIKQKEGSK 62

Query: 70  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 129
           ++  +K + +V+ LD NGK  SSE++AEL+ D G                          
Sbjct: 63  ILAHVKENMYVIALDLNGKMASSEELAELIGDLG-------------------------- 122

Query: 130 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 189
                         + G S ++  IGG  G  +++  RAN  +  S M   HQ+  ++L+
Sbjct: 123 --------------LKGKSNIALVIGGSLGLSKEVLNRANYKLSFSKMTFPHQLMRVILL 149

Query: 190 EQLYREY 193
           EQ+YR Y
Sbjct: 183 EQIYRAY 149

[Close]

BLAST of HG10013539 vs. ExPASy Swiss-Prot
Match: A0M0D0 (Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0803) OX=411154 GN=rlmH PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.5e-11
Identity = 52/188 (27.66%), Postives = 83/188 (44.15%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVE-----DVQLRSNPRNARDPRAQVDDEDM 69
           I+++ +GK   + ++ L+  Y  +L +Y   E     D++   N     D   Q   E  
Sbjct: 3   IKLVCIGKTDKKELEALIKIYSDRLQHYIKFEFEIIPDLKKTKN----LDENQQKSKEGE 62

Query: 70  AVVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSN 129
            +++ +++ D+VV+LDENGK+ SSE  +E +    NT                       
Sbjct: 63  LILSGVQNSDFVVLLDENGKQFSSESFSEYIQKRMNT----------------------- 122

Query: 130 YHCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVL 189
                            G  RL F IGGPYG  +++ +RA+  I LS M  +HQ+  L  
Sbjct: 123 -----------------GLKRLIFVIGGPYGFSEEVYKRADSKISLSKMTFSHQMVRLFF 146

Query: 190 MEQLYREY 193
            EQLYR +
Sbjct: 183 TEQLYRAF 146

[Close]

BLAST of HG10013539 vs. ExPASy Swiss-Prot
Match: B2V1Q9 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=rlmH PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.8e-11
Identity = 50/187 (26.74%), Postives = 89/187 (47.59%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLR--SNPRNA--RDPRAQVDDEDMA 69
           I +++VGK + + ++  +DEY ++L+ YC +  V+L+    P NA  +D     D E   
Sbjct: 3   ITIISVGKLKEKYLKHAIDEYSKRLSRYCKLNIVELQDEQTPDNASEKDELIIKDKEGNK 62

Query: 70  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 129
           ++N IK + +V+ LD  GK +SSE++++ + + G                          
Sbjct: 63  ILNSIKDNMYVITLDLKGKMISSEELSKFIDNCG-------------------------- 122

Query: 130 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 189
                         + G S L F IGG  G  + + +R+N S+  S M   HQ+  ++L+
Sbjct: 123 --------------VRGNSNLCFIIGGSLGLSEAVLKRSNYSLCFSKMTFPHQLFRVMLL 149

Query: 190 EQLYREY 193
           EQ+YR +
Sbjct: 183 EQIYRAF 149

[Close]

BLAST of HG10013539 vs. ExPASy Swiss-Prot
Match: Q1DCX5 (Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=rlmH PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 52/183 (28.42%), Postives = 80/183 (43.72%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMAVVNL 69
           +R+L++GK RS   +  V EY ++L +Y   E V++         P      E  A+++ 
Sbjct: 3   VRLLSIGKDRSGLYEPAVQEYAKRLGHYTRFELVEMAEASGKRLKPGDAKAAEAEAILSK 62

Query: 70  IKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNYHCAP 129
            K  DW+V LDE G  + S +++  V                                  
Sbjct: 63  RKPQDWLVALDERGSLLDSVELSRYV---------------------------------- 122

Query: 130 NVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLMEQLY 189
                   +A  GA  L F IGG  G   ++R  ANL++ LS M L H++A +VL+EQLY
Sbjct: 123 -------GKAQTGAKDLLFIIGGDEGLDARVRDAANLTLSLSKMTLPHRLARVVLIEQLY 144

Query: 190 REY 193
           R +
Sbjct: 183 RAF 144

[Close]

BLAST of HG10013539 vs. ExPASy TrEMBL
Match: A0A6J1FPF2 (putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111446064 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.4e-63
Identity = 137/187 (73.26%), Postives = 145/187 (77.54%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYI KLNNYC+VEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 85

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+KSDDWVVMLDENGKE+SSEQMAELVADAGNT                        
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNT------------------------ 145

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASRL+FCIGGPYGHGQQLRQRAN+SIKLSSMVLNHQIALLVLM
Sbjct: 146 ----------------GASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLM 172

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 206 EQLYRSW 172

[Close]

BLAST of HG10013539 vs. ExPASy TrEMBL
Match: A0A1S3CQZ8 (putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 3.1e-63
Identity = 136/187 (72.73%), Postives = 144/187 (77.01%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLLVDEYI KL NYCHVEDVQLRSNPRNARDP+AQ+DDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQIDDEDVA 85

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+KSDDWVVMLDENGKE+SSEQMAELVADAGNT                        
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNT------------------------ 145

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASRLSFCIGGPYGHGQ+LR+RANLSIKLSSMVLNHQIALLVLM
Sbjct: 146 ----------------GASRLSFCIGGPYGHGQKLRERANLSIKLSSMVLNHQIALLVLM 172

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 206 EQLYRSW 172

[Close]

BLAST of HG10013539 vs. ExPASy TrEMBL
Match: A0A6J1E0Z2 (putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.0e-62
Identity = 136/187 (72.73%), Postives = 145/187 (77.54%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQL+VDEYI+KL +YC+VEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 32  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 91

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+KSDDWVVMLDENGKE+SSE+MAELVADAGNT                        
Sbjct: 92  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNT------------------------ 151

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASRLSFCIGGPYGHGQQLRQRANL+IKLSSMVLNHQIALLVLM
Sbjct: 152 ----------------GASRLSFCIGGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLM 178

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 212 EQLYRSW 178

[Close]

BLAST of HG10013539 vs. ExPASy TrEMBL
Match: A0A6J1BPR4 (putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.0e-62
Identity = 136/187 (72.73%), Postives = 145/187 (77.54%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQL+VDEYI+KL +YC+VEDVQLRSNPRNARDPRAQVDDEDMA
Sbjct: 30  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 89

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+KSDDWVVMLDENGKE+SSE+MAELVADAGNT                        
Sbjct: 90  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNT------------------------ 149

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASRLSFCIGGPYGHGQQLRQRANL+IKLSSMVLNHQIALLVLM
Sbjct: 150 ----------------GASRLSFCIGGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLM 176

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 210 EQLYRSW 176

[Close]

BLAST of HG10013539 vs. ExPASy TrEMBL
Match: A0A6J1IWR3 (putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC111481296 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.4e-62
Identity = 135/187 (72.19%), Postives = 143/187 (76.47%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYI KLNNYC+VEDVQLRSNPRN RD RAQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNTRDARAQVDDEDIA 85

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           VVNL+KSDDWVVMLDENGKE+SSEQMAELVADAGNT                        
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNT------------------------ 145

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASRLSFCIGGPYGHGQQLRQRAN+SIKLSSMVLNHQIA+LVLM
Sbjct: 146 ----------------GASRLSFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIAVLVLM 172

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 206 EQLYRSW 172

[Close]

BLAST of HG10013539 vs. TAIR 10
Match: AT5G10620.1 (methyltransferases )

HSP 1 Score: 191.8 bits (486), Expect = 5.5e-49
Identity = 105/187 (56.15%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLVDEYIQKLNNYCHVEDVQLRSNPRNARDPRAQVDDEDMA 65
           RA+PIRV+TVGKKRS GV+LLVDEY  KL  YC  ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLIKSDDWVVMLDENGKEVSSEQMAELVADAGNTMARSRTNNMWIILGCLSLVKVSNY 125
           ++ LI SDDWVV+LDE G+++ SEQMAEL+ DAGN+                        
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNS------------------------ 156

Query: 126 HCAPNVSENNFNRAMGGASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLM 185
                           GASR+SFCIGG YGHG Q+R+RAN++I+LSSMVLNHQIAL+VLM
Sbjct: 157 ----------------GASRISFCIGGAYGHGTQVRKRANVTIRLSSMVLNHQIALVVLM 183

Query: 186 EQLYREY 193
           EQLYR +
Sbjct: 217 EQLYRSW 183

[Close]

BLAST of HG10013539 vs. TAIR 10
Match: AT5G10605.1 (methyltransferases )

HSP 1 Score: 55.5 bits (132), Expect = 6.2e-08
Identity = 27/36 (75.00%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 151 GGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLME 187
           G  YGHG+QLR+RAN++I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

[Close]

BLAST of HG10013539 vs. TAIR 10
Match: AT5G10605.2 (methyltransferases )

HSP 1 Score: 55.5 bits (132), Expect = 6.2e-08
Identity = 27/36 (75.00%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 151 GGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLME 187
           G  YGHG+QLR+RAN++I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

The following BLAST results are available for this feature:

BLAST of HG10013539 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136319.1	1.3e-71	80.21	putative RNA methyltransferase At5g10620 isoform X2 [Cucumis sativus] >KAE865137...	[more]
XP_031739112.1	6.3e-71	79.79	putative RNA methyltransferase At5g10620 isoform X1 [Cucumis sativus]	[more]
XP_038896820.1	8.5e-68	70.62	putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida]	[more]
XP_038896822.1	4.7e-66	73.96	putative RNA methyltransferase At5g10620 isoform X3 [Benincasa hispida]	[more]
KAG7037551.1	6.1e-66	73.58	putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of HG10013539 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXB4	7.8e-48	56.15	Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
A0PXE8	1.1e-12	27.81	Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT)...	[more]
A0M0D0	4.5e-11	27.66	Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0...	[more]
B2V1Q9	5.8e-11	26.74	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]
Q1DCX5	1.7e-10	28.42	Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK...	[more]

BLAST of HG10013539 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FPF2	1.4e-63	73.26	putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A1S3CQZ8	3.1e-63	72.73	putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770...	[more]
A0A6J1E0Z2	2.0e-62	72.73	putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=36...	[more]
A0A6J1BPR4	2.0e-62	72.73	putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=36...	[more]
A0A6J1IWR3	3.4e-62	72.19	putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of HG10013539 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10620.1	5.5e-49	56.15	methyltransferases	[more]
AT5G10605.1	6.2e-08	75.00	methyltransferases	[more]
AT5G10605.2	6.2e-08	75.00	methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003742	RNA methyltransferase RlmH	PFAM	PF02590	SPOUT_MTase	coord: 10..103 e-value: 1.2E-16 score: 61.1 coord: 138..191 e-value: 9.1E-17 score: 61.5
IPR003742	RNA methyltransferase RlmH	PANTHER	PTHR33603	METHYLTRANSFERASE	coord: 5..105 coord: 140..191
IPR003742	RNA methyltransferase RlmH	CDD	cd18081	RlmH-like	coord: 10..190 e-value: 1.32688E-45 score: 146.433
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 128..195 e-value: 7.9E-18 score: 66.5
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 7..115 e-value: 1.1E-16 score: 62.6
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 138..192
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 10..100

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10013539.1	HG10013539.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity