HG10013464 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10013464
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Isoamylase 3, chloroplastic
Location	Chr02: 1749822 .. 1757224 (-)
RNA-Seq Expression	HG10013464
Synteny	HG10013464

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATCCGTGGATTTACAGCTGATGAATCTAGTGGCTTGGCTTCAAGCACACAGGGAACTTACCTGGGTGTTATTGAAAAGGTAGAGATTACTGTGTTAAATCTTAGTGCCGTGATTGTCTGTGCTTTTCTTTTTCTTCGCCTTGTGGGACATCACAAGTTTTAATTTAGCAAGAACGGTGATGAAAGTATGATTGATGACACATGCATTAATTCTGCTTGTATCTGCTTTCAGTAGAGATAGCTTAGCAATGGTCTAATGATTTACTGCACTACATTCTCTATCATACTAATATTCTGACTAGAACCTAGAGAAGGAATGACTGTTGTGCATATAAATCGCAGAAGCTTCTAGGATGATGTAGATGACTTTTATATTTAATGTTGTAGACCTTCCATACTGCTCGGAATGGGAGAGTTGTCTTTGTGGCATACTATAATCTTTTTTAAACAGGAAACAGAAATTATATTGCCCAGGGAACAATTAGAAAAAAAGAGGAGATATCTCCCCACATGTCCAAATGGGTTACAAAAAGGCCTCCCAATTGGCAAAGATATGCAAAAAAAGCACAATTATAAAATTTAGTGGAAAGAAAACACCAAGAAGAAGAAAAAGTTTTCATTAGGTCCTACAAAGTCCCCTGTGCTTGACTCGATCCCCTTGATATTCTGTTGATCCGTTTCAAACCACAGCCTCTAGAGACTGGCGGCATCAGACTAGATCTAGCATGATTGTTGAATTGGGCCTCTAAAATAAGTTGAGTGAAGGAGCCATCAAAAGTCTTGGGGAAATGAACAATTAAGTCTTTATGGCATAATACAAAAATAAGATTTAGCATGTTAAGACGTCAATCTGTTTTAGCTTTATCATCAAAGGTGATATAGATGTATGTGTGCTTTGGAATTTTGTTCCATTTAGGCTAAATTTTTTTTCTTCAAACAGAAAAGAAAAAAAAAAAAAACTAAGTAACTACATTTGGTTTACGGATATGCTGGAAGCAAGGAATATGACCACTGTCCATCGCAGTTCGTGAGTGCCTAAACCCGGAGTTCGGTAGATTTTCTTGGTGAAAAATAATTTAGAACTCTTAGGTGTCTTAGAATCTTGTCAGCATATCAGTTATCAATGGGAGAATTGTTCTTTTGTTACGTAGCAATTCCTACTCCTATTGTTTATATTTTGTCTTCTTTTTTAGCCATTGCTCGTGTAGTGATTATTTTGGCTGATACAGTTTACATTCTAACTTCTTCTCTGGGTGACAGAATATACTAGCCCTTTATGGTTATTGTTTCTTGTATTTTCTTCCTCTGTTATATGTATAAACCTAGGCTGCAAACAGTATTTGTTGAATATTAACAGTGAAATAGTTTCTCTCATTGCCTGTTTATACAACCATTGCCAACTAAAAATTTATGTGTTGTTGATTGCCTTTTGCTAATAAAATGGTAAGATCCTGAATCTACTGAGGAAATGATTCATTTTCCATTTGATGCTCTGAAAATTTACTGGTAGTTCTCATTTTTCCTTGCCTTTTTTGCAATTCCCCTTCTATTGAGTTCTATATATCTAATCTTTTACCTTTCCAATTTTTAAGATTCCACATCTACTGGAGCTCGGTGTCAATGCAGTTGAATTATTGCCTGTCTTTGAGTTTGATGAGTTAGAGTTTCAGAGGCATCCAAACCCCAGGGATCATATGGTAAATTTTATAGAATTACTTGATTATCCTTTATGGCGATACGAACATTGTCATTATTTTTGTTGTGAAATGCTTAGATCAGAGGCCTTATACTACATTCTTTGTTTGAATTGGTGGTACCCACAAGCCTGAAAAATTGGTTCTTTAGTTTTTGTAGGTGTCATGGCTATAGAGATAGAGGAAATCTTTGTACTTCAAATGTGTATTTTTAGATAATTTTGGTACAAGAAAATTTCAATGAAGTTCATACTGATAAACTAAATCCCCTCTAAAAACCAAAAAGACTAAGAGACTAGTGGCTCAACTTAACTTGCAAATTCTGATACCCAAACACCTAAAATAATATGTTTCTCTTTCATTTCTTTGTGGGTGGGGCCCATGTGTCACTATTATCCATACTTCTTTTTTGTGATGATGATAAAAAATCTCAGAAAGGCATACGATTCAATTTTATGATTTAGAAATATGTCGCCGAACAAAATTTGAATAATAATTTGTATGTTTATTCTTTGTATCAAAATGAACTTTATACTCCATTGTCACTTTCTTTTTAACTTGGCCTGTATGCCTACTTTTTCTTAAAGATCAATACGTGGGGCTACTCCACAATCAACTTTTTTGCTCCTATGAGTCGATATGCTAGTGCTGGTGGAGGACCTCTTAATGCTTCGCGGGAATTTAAAGAAATGGTTAAAGCCTTGCATGCTGCTGGGATAGAGGTAAGTCATGTATTTAATCATTTATGTACACATAGATGGGATTTCTACATCTGATTCAGCTCTCAGTCACTTTGTTGGCAGTAAATTTCCTTAATATGTATTTTTTTTTCTCTATTTAATTTTGGAGTGAGGTCTTTTTCTTGTTGCAGGTCATTTTGGATGTTGTGTACAACCATACTAATGAGGCCGATGATGTAAATCCTTATACCACTTCATTTCGTGGCATTGATAATAAGGTGTTGTACTCATCTCTTTAAGTAATTTTACAAGTTTACATATATTAGCTTGGGTAATCTTACTAGTTTCTATCAACAGTAAATAGTATGTTCAAATTTTCCAAATTTTTCATTAACTTCTTTTGTAGGTTTATTACATGTTGGACCTAAAGAATAATGGTCAATATTTGAACTTCTCTGGCTGTGGTAAATTACTTTCTCTTGGATTATGCTTATACAAACAGAACCTTTACACTGAATACATAACTCTGTCTCTTCAATTTACATTCTTAAAATTTAGTTTTGAACCTTACTATAGTAGCTTTCTTTGAGTTTCCACTTCACGAGTAATTCTAGCCCAAGGCTTCCTAGTACTTCACTTACGCTTGGAGAATGGGTGGACGGATTTTCTTAAATTGTCATGCAAGATAGGATGGTAAACGAGTTAGGTGGATGCTTGGATCGGCATGTTGTTAGCTGGGGAATGGAGCAGTTAAGGACGACTTTCAGGAGAGGCAGTGAAGGCTCCTAGTTCTTTCTAGTTCTATCATATTAGGACGGGCTTTCATTTTCTACATTAAAATGTCAGAAATACAATATTTCCATCAAGGGACATAAACTCATGGGCTCTCTCTTAATTATTTATTATTTTATTATTATTATTATTATTTTTTTTTGTTGAAGTAGGGAAAAGGTTCAAACGGGAAGTAAGGGAGAGAGTTTTTATCTATGTTGGTTATAGCGTGGAAAATCCCCTGTGGAACTTATGTAGCTTGAACATTGTTTACGGTTGCTACAAGGTAGCCTGCTTGTGTTTGAAGAAATATATTACAGGGCTCCTACAAAATAGCTATACCTTATCTACTTGCTCAAAGAGGAGATCTAATTTTGGGGGTGGGGGAGGAGAGTTGATGTAGGAGGACTACTTTACTTACAGGCCTTGTACTTCTATGTAGTTGTTCCTGGCAATTAATGCTGATTGATTAATTGAGATATATTGCCATTAATATATAGTTCTTTGGCTAGTTAGGTAAGTAACTGCCTAGCCGACCAGGGACCGTATGTAGCCAGTCAGAGATGCTATTAAGTTGACGGAGAGGGCTGCAATTATCAGATTATTCTGGATTCCAAAAATATATAATTCAAATATATTCTATCTTTTCAAATATTTTTGTAACAAGAAACAAAATTGTCAACCATTGATGGTTGAAATGTTACAACATGTTGAATAAAACAAGCTCCCTTTTGGAGTGGAGAAAAAAGAACCATTCCTCCTAAAAATATATGAAAATACACAAAAAGGGCCCCAAATTGAGATAAATACCATTTGTTTTAAAATGCAATAACATGTTAGTAGTCTTGAGTTACCTGATTTCCTTTTTCTGCAGGCAACACCTTAAACTGTAATCATCCCGTGGTGATGGAACTAATTCTTGACAGCTTAAGACACTGGTATGGACTTCACACTAGCTTCCTCAGAAACTTATTTGGAAGAAATTGACGTATTAGACTGTGAAACGTGCTTTTCAGTTTTCTGTAAATATTGTTTTGTACACATAACCTTTTATTATTATTATTATTTTTTTTATATCCGTGAGTATCCGGGCCAGCTTACGCGTACCTCGACTAATCTCACGAGACAACCTGCCTGACCCTATAACATTTGGGTGTCAAGGAAACTCATAGGAAAGTTTTTATTGATATATATGAACCCTATCCAAAGGAGTTAAAAATACTTCTCCAACTGACAAAAGATTAGAAAAGTATATGAGTGAAAAGAAAAAGGAATTTACACCTAGTCACTGCAAGAAACATAAAATGATCGAAAAAGCTCTCAAATGATAGCTCGATGTCTCCAAAAAGATGGTGATTTCTCTCGAGTCTTGATCCAAACCAACCAAAAGAAAGGCCTGGTTAATGTTCAACCAAAGGAGCTCTTTCTCCTTTTTACATGGATGCCCAAGCAAATGAGCAACAGCACAACTTTCCTCGTACAGACACCTTATAGTTTTAAGTATGTAATTTTGTGTCATATTCTACCATGTACATACTCCATTTTATGCTATTGAGATGGGAAAAACTTCATTGATTCGAAGTCGGTTGCTGCTGCTTAGGATTTAATGTGCGTAAAAAGATAAGAAGCAAAGGAAGCTTAGAAATCATTATTTGCCCCCCTTTGGTGTGTTCATTATGCTTTATGGGGGAGGAATCTAGTAACCACCAGTTCCTTAAATGCAACTTTGCTAGAAGGGGCTGGGAGCAACTTTTTAAAGCTCTTGACTTAAAAATTTGTCTTTGGGAGGAGATGGGTAGTCGGCTTTTGGAAGTTCTATTTTGGTGGAACTTTAAAGGTAAAGCAAAAATTCTTTGCGGGTGTGTGGCTCGGGCTCTTTTGTGGGAGCTTTTTGAAGATAAGTCTTAAGTCTTTTGTTGATTTTCATAACCTTGTACAACATACTTTCTTTTCATGGACTCTTAGAGAAAAAGGTTTCTTCTGTAATTATAATCTTGCTCAAACCCTCCAGAGTTGGGAGGCTTTGTTGCATAGTTCTCTTGGGAGAGGGGCTTTTCGCTTCTCTGCCCTCAGGCTCTCTCTCCTTCTCTATATATATTTTGTGATTAATACAGTTTTTTTTTTCTTATCAAAACAAAAACAAAAAACAATATTAAAGAAAAGAGTTATAGATGATATCTTGTAACTGAACTACATATTTGTTTATCTCACGATACTCAATTTCAGGGTGGTTGAATATCATGTAGATGGTTTTAGATTTGATCTTGCTAGTGTTCTTTGCCGGGGAACAGATGGTGCTCCACTTAGTGCTCCCCCACTCATAAGGGTATGTAATTTTGTGTACGTGGTTTTTTGTTAACCTACATACATGACATTTTATTCTATATCTGGAAGGAGAGAAACTAGAACATTTTGCATAAAAAACTCATTGCTGCGTGTATTTAACTTCTTTGTGGGTCTTTTCAATAAAGTAGTCTGTTTTTAGTTTCTATTTTTATTATGATAAGTCTAGCAGGAAAGGTTGTATAGTCAATGGAGGGATAGTATAATAAGTGTGCATTTGCTAAGAGTTTGATAAAGACCACCAGCTATAAAAATAGAGAAATGGGGTCTGACTGGTAAGGTAAGATCGATAATTTCGTGACAGTTTGGTGTGAGCTTGCTTTTAAATTATTTGAATTCCTTGAGACTGTATTCATCTTTCTATACAATTTCAAATCTCTTTCCTATTCCTATCTCTTTTTATTATTCAAATTTGCAATTTTTAGAATCTCTAATTACTTATACAGGTTGTCCCTAAAAAAAGGAAAAATCTCTAATTACTTATACATTATATTTTTGGGACAATCAGTGATTTTAACATGGTTTCAAAGAAGGGGGTCCAGGAGATCATGTGTTCAAACCCGTGCAATGTTATTTCCTCTTAATTAATATTGATTTCTATTTGTTGAGTCTTTTACATATTTCAAGCTCACAAGTGTGGGAGAGTATTAAAGTTTTGATATAATATTAAATTTACCTTCACTTTTTTTTTTTTAGTATCAACAACTATGGCTAGGTGGGGATCAAACCTTCAAACTCTAGGGAGGAAGGTCATGCCAATTACCATTGAGCTAAGCTCACTTTGGCAATTTACCTTCACTCATCAGCTTAAGCTTTTGGGTCAATCGATGATTTAACATTTCCTATCAATAAAAAAAAAGGCTAAAATATCATTTTGGTCCGTGTAATTTGAGTTTCATTCCATATTATTCCTATACTTTTAAATGTCCAAAATTAGTCTTGTACTTTCAATAAGTCATAAATTAGGTAATCAAAAAATGCTCTCTCCTCTCCCATTCCCCCCTCTCAGCCCATATCTTCCCTTTTCTAACTCCGACCACCATGCCTCAACCACCGATGACCACCACTCCTTCCACTATCCGATCCATCCATATAGACAGAAAACTCTTCTCCTTATTCCCGCTAGCATCAAACAATAGAAAACATTTCAAACTATCGGAACAAAGTCACTCCAACATACACTCGATTTTTGTTTCAAAGAATTCTCTTGAATGGCTTGCCTCCTCATTCAACTCTCTCTTCAAAGAACCGTGCAACCAAAATTTTTTCAAGAAGTTAAACGATCAGGGAGACACTTTTTGGATAGAAAAGCGCAGCAACAAGTATGGCTTTTTTGCAGAGATTTCCTTGCTGGAACAGTCAGGCCACCGTACAAATATCCTCATCCCTGCCATGGAGGAAAAAAGAGGTGGTTCTCTTTCTTATCTTTGATATCAGACTACCCGAGAAGGCCACCACATCCAGCTTCTCCATCACACCACAAACCACCAACCCAAAATCATACAGTGAAGTTGTTAAACCACAAGCCATCAACCAACAATCCGAACAGATTATATCAAGCTCTTCACCAATCCTCCCTCAATTCGATTGGAAGGCTATGATAATTGTCTAGCGGTTCTCGAGCAGCGATACATGGTCAGATATTCGTGCAGCTCTTCAAGTTTCCCTCTCAACCCGTTGTACTATTAATCCCATCTCGGACGACAAAGCATTGCTCCATGTATACAACAAGGAATTAGCTCAAGACTTGTGCAAAAAGTTGGATTGGACAACCATAGAACAGAGGAAACTCAAATTTCTCTCACCAACCGATCCATCATCATCAGCAACAAATGATAGCAACAAAGGGAGGATGGATCGACATCTACCACCTACCAATACTCATGGACAGATGCAATTCAAATATATAG

mRNA sequence

ATGAATATCCGTGGATTTACAGCTGATGAATCTAGTGGCTTGGCTTCAAGCACACAGGGAACTTACCTGGGTGTTATTGAAAAGATTCCACATCTACTGGAGCTCGGTGTCAATGCAGTTGAATTATTGCCTGTCTTTGAGTTTGATGAGTTAGAGTTTCAGAGGCATCCAAACCCCAGGGATCATATGATCAATACGTGGGGCTACTCCACAATCAACTTTTTTGCTCCTATGAGTCGATATGCTAGTGCTGGTGGAGGACCTCTTAATGCTTCGCGGGAATTTAAAGAAATGGTTAAAGCCTTGCATGCTGCTGGGATAGAGGTCATTTTGGATGTTGTGTACAACCATACTAATGAGGCCGATGATGTAAATCCTTATACCACTTCATTTCGTGGCATTGATAATAAGGTTTATTACATGTTGGACCTAAAGAATAATGGTCAATATTTGAACTTCTCTGGCTGTGGCAACACCTTAAACTGTAATCATCCCGTGGTGATGGAACTAATTCTTGACAGCTTAAGACACTGGGTGGTTGAATATCATGTAGATGGTTTTAGATTTGATCTTGCTAGTGTTCTTTGCCGGGGAACAGATGGTGCTCCACTTAGTGCTCCCCCACTCATAAGGATCCATCATCATCAGCAACAAATGATAGCAACAAAGGGAGGATGGATCGACATCTACCACCTACCAATACTCATGGACAGATGCAATTCAAATATATAG

Coding sequence (CDS)

ATGAATATCCGTGGATTTACAGCTGATGAATCTAGTGGCTTGGCTTCAAGCACACAGGGAACTTACCTGGGTGTTATTGAAAAGATTCCACATCTACTGGAGCTCGGTGTCAATGCAGTTGAATTATTGCCTGTCTTTGAGTTTGATGAGTTAGAGTTTCAGAGGCATCCAAACCCCAGGGATCATATGATCAATACGTGGGGCTACTCCACAATCAACTTTTTTGCTCCTATGAGTCGATATGCTAGTGCTGGTGGAGGACCTCTTAATGCTTCGCGGGAATTTAAAGAAATGGTTAAAGCCTTGCATGCTGCTGGGATAGAGGTCATTTTGGATGTTGTGTACAACCATACTAATGAGGCCGATGATGTAAATCCTTATACCACTTCATTTCGTGGCATTGATAATAAGGTTTATTACATGTTGGACCTAAAGAATAATGGTCAATATTTGAACTTCTCTGGCTGTGGCAACACCTTAAACTGTAATCATCCCGTGGTGATGGAACTAATTCTTGACAGCTTAAGACACTGGGTGGTTGAATATCATGTAGATGGTTTTAGATTTGATCTTGCTAGTGTTCTTTGCCGGGGAACAGATGGTGCTCCACTTAGTGCTCCCCCACTCATAAGGATCCATCATCATCAGCAACAAATGATAGCAACAAAGGGAGGATGGATCGACATCTACCACCTACCAATACTCATGGACAGATGCAATTCAAATATATAG

Protein sequence

MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRIHHHQQQMIATKGGWIDIYHLPILMDRCNSNI

Homology

[Close]

BLAST of HG10013464 vs. NCBI nr
Match: XP_038896292.1 (isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoamylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-117
Identity = 205/211 (97.16%), Postives = 210/211 (99.53%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGLASST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 242 MNVRGFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 301

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMIN WGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE
Sbjct: 302 DHMINMWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 361

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADDVNPYTTSFRGIDNKVYYMLDL+NNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV
Sbjct: 362 ADDVNPYTTSFRGIDNKVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 421

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           +YHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 422 DYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 452

[Close]

BLAST of HG10013464 vs. NCBI nr
Match: XP_038896295.1 (isoamylase 3, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 433.3 bits (1113), Expect = 1.3e-117
Identity = 205/211 (97.16%), Postives = 210/211 (99.53%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGLASST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 154 MNVRGFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 213

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMIN WGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE
Sbjct: 214 DHMINMWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 273

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADDVNPYTTSFRGIDNKVYYMLDL+NNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV
Sbjct: 274 ADDVNPYTTSFRGIDNKVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 333

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           +YHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 334 DYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 364

[Close]

BLAST of HG10013464 vs. NCBI nr
Match: XP_008466196.1 (PREDICTED: isoamylase 3, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 428.7 bits (1101), Expect = 3.3e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 154 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 213

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 214 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 273

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 274 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 333

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 334 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 364

[Close]

BLAST of HG10013464 vs. NCBI nr
Match: XP_008466194.1 (PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >XP_008466195.1 PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >TYK31297.1 isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 428.7 bits (1101), Expect = 3.3e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 243 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 302

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 303 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 362

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 363 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 422

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 423 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 453

[Close]

BLAST of HG10013464 vs. NCBI nr
Match: KAA0038687.1 (isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 428.7 bits (1101), Expect = 3.3e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 244 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 303

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 304 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 363

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 364 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 423

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 424 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 454

[Close]

BLAST of HG10013464 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 4.7e-105
Identity = 176/211 (83.41%), Postives = 194/211 (91.94%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+R FTADESSGL S+++G+YLG+I+KIPHLLELGVNAVELLPVFE+DELEF+R+PNPR
Sbjct: 256 MNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPR 315

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHM+NTWGYSTINFFAPMSRYASAGGGP+ AS+E K+MVK LH AGIEVILDVVYNHTNE
Sbjct: 316 DHMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNE 375

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADD +PY TSFRGIDNKVYYMLDL  N + LNFSGCGNTLNCNHPVV ELILDSLRHWV 
Sbjct: 376 ADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVE 435

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYH+DGFRFDLASVLCRG DG PL APPLI+
Sbjct: 436 EYHIDGFRFDLASVLCRGPDGCPLDAPPLIK 466

[Close]

BLAST of HG10013464 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 1.4e-101
Identity = 176/211 (83.41%), Postives = 188/211 (89.10%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+R FTADESSG+  +  G+YLG IEKIPHL +LG+NAVELLPVFEFDELE QR  NPR
Sbjct: 241 MNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPR 300

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFKEMVKALH+AGIEVILDVVYNHTNE
Sbjct: 301 DHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNE 360

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADD  PYTTSFRGIDNKVYYMLD   N Q LNFSGCGNTLNCNHPVVMELILDSLRHWV 
Sbjct: 361 ADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVT 420

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCR TDG+PLSAPPLIR
Sbjct: 421 EYHVDGFRFDLASVLCRATDGSPLSAPPLIR 449

[Close]

BLAST of HG10013464 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.5e-58
Identity = 120/232 (51.72%), Postives = 146/232 (62.93%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN-P 60
           M++RGFT  ESS +     GTY GV EK+ HL ELG+N +EL+P  EF+ELE+  +    
Sbjct: 223 MHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTIL 282

Query: 61  RDHMINTWGYSTINFFAPMSRYASAGGGPL--NASREFKEMVKALHAAGIEVILDVVYNH 120
            DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI+DVV NH
Sbjct: 283 GDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNH 342

Query: 121 TNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRH 180
           T E ++  P   SFRG+DN VYYML  K  G++ N+SGCGNT NCNHPVV + ILD LR+
Sbjct: 343 TAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQFILDCLRY 402

Query: 181 WVVEYHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLI 211
           WV E HVDGFRFDL S++ R                    T G P+S PP+I
Sbjct: 403 WVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVI 449

[Close]

BLAST of HG10013464 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-56
Identity = 121/233 (51.93%), Postives = 147/233 (63.09%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           M++RGFT   SS +     GTY+G I K+ +L ELGVN VEL+P  EF+ELE+    +  
Sbjct: 250 MHLRGFTKHSSSNV--EHPGTYIGAISKLDYLKELGVNCVELMPCHEFNELEYFSCSS-- 309

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASR----EFKEMVKALHAAGIEVILDVVYN 120
              +N WGYSTINFF+PM RY+S  GG  N  R    EFK  V+  H  GIEVI+DVV+N
Sbjct: 310 --KMNFWGYSTINFFSPMIRYSS--GGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFN 369

Query: 121 HTNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLR 180
           HT E ++  P   SFRGIDN  YYML  K  G++ N+SGCGNT NCNHPVV E I+D LR
Sbjct: 370 HTAEGNEKGP-ILSFRGIDNSTYYMLAPK--GEFYNYSGCGNTFNCNHPVVREFIVDCLR 429

Query: 181 HWVVEYHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLI 211
           +WV E HVDGFRFDLAS++ RG                   T G PL+ PPLI
Sbjct: 430 YWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATPPLI 471

[Close]

BLAST of HG10013464 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.1e-41
Identity = 99/198 (50.00%), Postives = 126/198 (63.64%), Query Frame = 0


Query: 2   NIRGFTADESSGLASSTQGTYLGVIEK--IPHLLELGVNAVELLPVFEFDELEFQRHPNP 61
           +++G T    S +    +GTY G+     I HL EL V AVEL+PV +F  L   R  + 
Sbjct: 179 HVKGMTQTHPS-IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF--LHDSRLLDL 238

Query: 62  RDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTN 121
              + N WGY+T  FFAP  +YAS      +A  EFK MV++LH AGIEVILDVVYNHT 
Sbjct: 239 --GLRNYWGYNTFGFFAPHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNHTA 298

Query: 122 EADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWV 181
           E + + P T +FRGIDN  YY L   +   Y +F+G GN+LN  HP  ++LI+DSLR+WV
Sbjct: 299 EGNHLGP-TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWV 358

Query: 182 VEYHVDGFRFDLASVLCR 198
           +E HVDGFRFDLAS L R
Sbjct: 359 IEMHVDGFRFDLASTLAR 369

[Close]

BLAST of HG10013464 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.6e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 243 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 302

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 303 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 362

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 363 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 422

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 423 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 453

[Close]

BLAST of HG10013464 vs. ExPASy TrEMBL
Match: A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.6e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 244 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 303

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 304 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 363

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 364 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 423

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 424 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 454

[Close]

BLAST of HG10013464 vs. ExPASy TrEMBL
Match: A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.6e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 243 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 302

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 303 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 362

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 363 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 422

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 423 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 453

[Close]

BLAST of HG10013464 vs. ExPASy TrEMBL
Match: A0A1S3CQN6 (isoamylase 3, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.6e-116
Identity = 202/211 (95.73%), Postives = 208/211 (98.58%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 154 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 213

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 214 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNE 273

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
            DDVNPYTTSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 274 GDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 333

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 334 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 364

[Close]

BLAST of HG10013464 vs. ExPASy TrEMBL
Match: A0A0A0LE02 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.8e-115
Identity = 201/211 (95.26%), Postives = 206/211 (97.63%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+RGFTADESSGL SST+G+YLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR
Sbjct: 244 MNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 303

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFK+MVKALHAAGIEVILDVVYNHTNE
Sbjct: 304 DHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVILDVVYNHTNE 363

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADDV PY TSFRGIDNKVYYMLDLKNNGQY NFSGCGNTLNCNHPVVMELIL+SLRHWVV
Sbjct: 364 ADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVV 423

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR
Sbjct: 424 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 454

[Close]

BLAST of HG10013464 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 370.5 bits (950), Expect = 1.0e-102
Identity = 176/211 (83.41%), Postives = 188/211 (89.10%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           MN+R FTADESSG+  +  G+YLG IEKIPHL +LG+NAVELLPVFEFDELE QR  NPR
Sbjct: 241 MNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPR 300

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNE 120
           DHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFKEMVKALH+AGIEVILDVVYNHTNE
Sbjct: 301 DHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNE 360

Query: 121 ADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVV 180
           ADD  PYTTSFRGIDNKVYYMLD   N Q LNFSGCGNTLNCNHPVVMELILDSLRHWV 
Sbjct: 361 ADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVT 420

Query: 181 EYHVDGFRFDLASVLCRGTDGAPLSAPPLIR 212
           EYHVDGFRFDLASVLCR TDG+PLSAPPLIR
Sbjct: 421 EYHVDGFRFDLASVLCRATDGSPLSAPPLIR 449

[Close]

BLAST of HG10013464 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 226.9 bits (577), Expect = 1.8e-59
Identity = 120/232 (51.72%), Postives = 146/232 (62.93%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN-P 60
           M++RGFT  ESS +     GTY GV EK+ HL ELG+N +EL+P  EF+ELE+  +    
Sbjct: 223 MHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNTIL 282

Query: 61  RDHMINTWGYSTINFFAPMSRYASAGGGPL--NASREFKEMVKALHAAGIEVILDVVYNH 120
            DH +N WGYSTI FF+PM RYASA        A  EFK +VK  H  GIEVI+DVV NH
Sbjct: 283 GDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNH 342

Query: 121 TNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLRH 180
           T E ++  P   SFRG+DN VYYML  K  G++ N+SGCGNT NCNHPVV + ILD LR+
Sbjct: 343 TAEGNEKGP-IFSFRGVDNSVYYMLAPK--GEFYNYSGCGNTFNCNHPVVRQFILDCLRY 402

Query: 181 WVVEYHVDGFRFDLASVLCRG-------------------TDGAPLSAPPLI 211
           WV E HVDGFRFDL S++ R                    T G P+S PP+I
Sbjct: 403 WVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVI 449

[Close]

BLAST of HG10013464 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 153.7 bits (387), Expect = 1.9e-37
Identity = 92/214 (42.99%), Postives = 120/214 (56.07%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           +N++GFT   SS L S+  GT+ GV EK+ HL  LG NAV L P+F F E   Q+ P   
Sbjct: 369 LNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSE---QKGP--- 428

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKEMVKALHAAGIEVILDVVYN 120
                   Y   +FF+PM  Y     GP N    A    K MVK LH+ GIEV+L+VV+ 
Sbjct: 429 --------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFT 488

Query: 121 HTNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLR 180
           HT ++        + RGID+  YY     N+    ++      LNCN+PVV +L+L+SLR
Sbjct: 489 HTADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYPVVQQLVLESLR 548

Query: 181 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLI 211
           +WV E+HVDGF F  AS L RG  G  LS PPL+
Sbjct: 549 YWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLV 550

[Close]

BLAST of HG10013464 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 153.7 bits (387), Expect = 1.9e-37
Identity = 92/214 (42.99%), Postives = 120/214 (56.07%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 60
           +N++GFT   SS L S+  GT+ GV EK+ HL  LG NAV L P+F F E   Q+ P   
Sbjct: 369 LNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSE---QKGP--- 428

Query: 61  DHMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKEMVKALHAAGIEVILDVVYN 120
                   Y   +FF+PM  Y     GP N    A    K MVK LH+ GIEV+L+VV+ 
Sbjct: 429 --------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFT 488

Query: 121 HTNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQYLNFSGCGNTLNCNHPVVMELILDSLR 180
           HT ++        + RGID+  YY     N+    ++      LNCN+PVV +L+L+SLR
Sbjct: 489 HTADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYPVVQQLVLESLR 548

Query: 181 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLI 211
           +WV E+HVDGF F  AS L RG  G  LS PPL+
Sbjct: 549 YWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLV 550

[Close]

BLAST of HG10013464 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-19
Identity = 64/230 (27.83%), Postives = 110/230 (47.83%), Query Frame = 0


Query: 1   MNIRGFTADESSGLASSTQGTYLGVIEK-------IPHLLELGVNAVELLPVFEFDELEF 60
           +++R F+A++ + +    +G YL    K       +  L++ G+  + LLP F+F +++ 
Sbjct: 338 LHVRDFSANDET-VEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDD 397

Query: 61  QR--------------HPNPRDHMIN----------TWGYSTINFFAPMSRYASAGGGPL 120
           ++               P+  +               WGY+ + +  P   YAS   GP 
Sbjct: 398 EKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPC 457

Query: 121 NASREFKEMVKALHAAGIEVILDVVYNHTNEADDVNPYTTSFRGIDNKVYYMLDLKNNGQ 180
               EF++MV+AL+  G+ V+LDVVYNH + +   +  +   + +    YY+   +N+  
Sbjct: 458 RII-EFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPG--YYL--RRNSDG 517

Query: 181 YLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCRGT 200
           ++  S C N     H +V  LI D L +WVV Y VDGFRFDL   + + T
Sbjct: 518 FIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKAT 561

The following BLAST results are available for this feature:

BLAST of HG10013464 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896292.1	1.3e-117	97.16	isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoam...	[more]
XP_038896295.1	1.3e-117	97.16	isoamylase 3, chloroplastic isoform X2 [Benincasa hispida]	[more]
XP_008466196.1	3.3e-116	95.73	PREDICTED: isoamylase 3, chloroplastic isoform X2 [Cucumis melo]	[more]
XP_008466194.1	3.3e-116	95.73	PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >XP_008466195.1...	[more]
KAA0038687.1	3.3e-116	95.73	isoamylase 3 [Cucumis melo var. makuwa]	[more]

BLAST of HG10013464 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9G434	4.7e-105	83.41	Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=...	[more]
Q9M0S5	1.4e-101	83.41	Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2	[more]
O04196	2.5e-58	51.72	Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1	[more]
D0TZF0	1.1e-56	51.93	Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=...	[more]
P0A4Y5	2.1e-41	50.00	Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935...	[more]

BLAST of HG10013464 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CS22	1.6e-116	95.73	isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 P...	[more]
A0A5A7TAT5	1.6e-116	95.73	Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 P...	[more]
A0A5D3E5B0	1.6e-116	95.73	Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 ...	[more]
A0A1S3CQN6	1.6e-116	95.73	isoamylase 3, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503684 P...	[more]
A0A0A0LE02	6.8e-115	95.26	Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 S...	[more]

BLAST of HG10013464 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G09020.1	1.0e-102	83.41	isoamylase 3	[more]
AT2G39930.1	1.8e-59	51.72	isoamylase 1	[more]
AT1G03310.2	1.9e-37	42.99	debranching enzyme 1	[more]
AT1G03310.1	1.9e-37	42.99	debranching enzyme 1	[more]
AT5G04360.1	1.1e-19	27.83	limit dextrinase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 3..240 e-value: 0.0058 score: -87.9
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 20..197 e-value: 1.8E-14 score: 54.1
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..226 e-value: 9.7E-82 score: 277.2
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 1..223
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 2..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10013464.1	HG10013464.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0019156	isoamylase activity
molecular_function	GO:0003824	catalytic activity