HG10013428 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013428
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRhamnogalacturonan endolyase
LocationChr02: 1379478 .. 1385161 (+)
RNA-Seq ExpressionHG10013428
SyntenyHG10013428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATGAGATACTTCAATTGGATATTCAGGATCATCATGTAAGTCATGGTTATTTCTTTCTGCTGTTCAATATGACTTAAATTGGTTTACGAGACGAGAAGATGTGTTTTGCATTTGTGTGGGCTGCAGGTAGTTATGGACAATGGTATATTGCAATTGACACTGTCCAAGCCAGATGGAAATGTAACTGGGATAAAATACAACAGCATTGACAATGTTTTAGAAGTTGATGATAAGGAAGCTCATAGAGGGTATATGATTTCATTTACTACTATCAAATCTCTCTATCTTTCTGCAATTATGATAGTCTAGTTAATGCCAAACATTATGTTTACTCAGATTGACTGATGAAATATGATGCAGGTACTGGGACCTCGTGTGGAACTCTGGGACTCCGGGAACCACGGGAACGTTCGATGAGTATGCCGAAACTAACACTAATTATTAAAATGACTTTTCAATTTATTGTTTTGTTTCATTTCAAGTCTGGATAGTCTTCTATTCTCAGATTTATTAATGACGGGCTTTCTTTAATTCTACTTAAAGAAACTTTAATTTATAGCTGAAATGGTCTCAAGACATTTAAAATGCTTTCAGGATGAGAGGAAAAGAATTTGAAGTGATTGTGCAAAATGAGGAACAAATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCTGTTCCCTTAAACATAGACAAAAGGTGCCTGCAATCTGCATCATGAATCTAATGAACTGTTGTCCTTATGTTAAGTTCTTAAATAATGACTCGAAAAAGACATACTAAAACTGATAAGAAGTCCTTTTTCAGGTTCATAATGCTTCGTAACTCCTCTGGATTCTACTCCTATGCCATCTATGAGCACATGAGGGAATGGCCACCATTCAACCTTCCTCAAACAAGGATTGTGTTTAAGCTAAGAAAGGACAAGTACGACATCATATGAATACCTTTTAATTTAAGCACTTTTGAAAGAAAAAAATATTTTCAATAAAAATGTTATTTTATTTTACATTTTTTCAAAACTATTTTAAATGATGTTTGGTTGTACTATAGATTCTATCAATTATAAACATGTTTTCACATATTTTGAAGATTTTCGGTTCATCACTTTAAAGTCATTTTTGAGAATTCATCAGCCAATCAAAGACGATATTAAAGTGATCCGATTTTTTTTGAAAAAAAATAAAGTTTGATCATTTAAAATCACTAAATAAGCTATATTAAACATACTCTTCGATTGGTTGTGCAGTGAATCATTAATTAATTGTGATGTTTTTTTTTTCTTTCATTGTAATATAGGTTTCATTATATGGCCATAGCAGATGACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTACGTCACAGAGGAAAACCTTTGGATTTTCCAGAGGCTGTATTGCTAGTCAACCCTAAAGAACCTGAGTTCAAGGGAGAGGTTCTTCTACTTCTCATGACATTGCTTTGTCTGACTCATAATTTTCTGTTCCTTTATACTGAAATCCATAACTTCTTTTATATTAGTATTGAAGAACATAACATCCAATTATATATGCTTGAGCTCAAACATTTGCATATTTATGTAAGAAGTCTCTAAATTAAACATATTTAGGAGTTTTCATCCTTCTTATATGCTGTTAAAGTATTGATATAAATAAATTGATTGTAACACATCAACTTAAGCTCTCGAACTCAATAATGATTTAACATAGTACCAGAGTAAGAGGTCTTATGTTCAAACTCCTGTAATGTCATTTTCCCTCCAAGTAACATTGATTTCCACTTATTGGGTTTTCTACAAATTTTTAAGTCCACAAGTGAGGTAGAGCGTTAGAGTATTAATATAATTAAATTGACTGTAACTCATCAACATAAGCTTTTGAATTTAGTGGGAATTAAACCTACTCTCGAAACCAATTGCTTTGATGGACCAAGCTAAGTTTGTAAGAATCTGATATCATTTAACATGTGCCTATGAGTAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTCCGGTGGGTTTCTGGCAAATCACTCCAAGCAGTGAGTTCAGATCAGGCGGACCCCTCAAACAGAACCTTACCTCTCACGTGGGCCCATATGCTCTTGCTGTAAGTATATATCATTTTTGGTAAATGAGCAAGTTTAAGTTAATTGAAGAAGTTAAACATGAATTAAGTAATGACATATTAATTAGGTTTTTTTTTTGGGTAAGAAACAGAGCTTTCATTAAGAGAAATGAAAAAACATAAAAGGGAATACAAAATGCGTATTGATAAATCAAAATGCTACAATTGCAGATGTTTCTTAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAGCTCAGTCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTTTCTATCTAAATTCTGTTTCTGATGGAGGTGATCCACTTTCTTTGTGGGAAGATGCTAAAACTCAGGTTGTAATGATGAAGCTGGATTTTGATTATGAATATATTTTAATTTTGATCTTGAAAATGGCTGTTCATGACTCAACTTTGCCTTTAAATTTATCTTCTGCATAACTATTTTTCAGATGACGAAGGAAGTTCAAAGCTGGCCCTATTCTTTTCCAGCTTCTGAGGATTTTCCCCCACCAAACCAACGTGGTAGATTGACTGGCAAATTATTTGTACTTGACAGGTAGATTTATACGTACGTACATACATGCATAAAAACACACATGCAAAAGTTAACCACTTGTAGAATTATCTATAGCCAACCTTTTCTGTTCATTAATAATAAAATGAAAAATTTGTACGAGCAACTAAGGTTAAAATACCATTTTGGTTCATGTATTTTGAGTTTCACTCAATTTTAGTTTTTGTACTTTCAAATGTCAAAATGTAATCCTTGTACTTTTAATAAATCTTAGTTTAGTCCTTTGAAGTTATTATTATTATTTGTTAAAATTGGTTAATTTTCATGTAAGAAAAGAGAGTATATTTCTAAAATTTATAAAAAGAAGATTAATTGAGGCAATAATTTTTTTTTCCTAAGAAAAATCAACTATAAATTAATAGTATGGACTATTTTTAAGATTTATTGAAACTAATAAGGACTAAATTTAGACGTTTAAAAGTACAAAGACTAAAACTGGAGAAAAATTTCAAAGTATAAAGACCAAACTAATATTTTACGTAACTTGTAATTTGATAGGAAAGTTTAGGACTAAATAATTTGCAATACATTAAACTTGTATGCAGATATGTTAACCAAGAGCAGACGCCGGCAACTGACGCGTATGTGGGACTGGCAGCACCAGGAGATGCTGGATCATGGCAAACTGAAAACAAGGTACTGAGGATTGATGAAAAATGCTCGACAAACACTTTGAAGTTCGAACTTTCGAAGTGCATTACATATCAAACAATGACAGCAAAGAAAATGATGTTATTTGAACCATGATCAGGGTTACCAGTTTTGGACCAAAACAGATGCAGATGGCTCGTTTAACATTGAACACGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTATGATGTTGTCATTAGTGTCTCTGCAGGTTTAATTCATTTCACTTTCTAAAAATTCTGCATTTGTATATTCACATCATTAACTGTTAAAATGATTACTGAATCGATTTATAACTCAATTGGTTAAAACATATATCATCGACTGAGGTTAGATGTTCAAGGAAAGCTTATAATTCTATTTCAGCAATTATAATCGTATTGATGTTCGATTTCAGGTAGTGATATGGATGTAGGTGATCTTGTTTATGAGCCGCCTAGAAACGGGCCCACATTGTGGGAAATAGGCATTCCCGATCGCTCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAAGTACATTAACAAATTGTATGTCAATCATCCAGATAGGTACGAGACCTTTTCTTTTCTTCAGAATATTGTTTCAGACTTTCAGTTATGGTAATCGCTTGCTTTAGACCTTAGTCCTCACAATTATACTCTGATTTGTTAATACATCATTAATTTGGAGGAAGGATTATTACATAAATGTAAGCTCAAGATTTCTTCTCATCCCAATATGTCTCTAAATCATCAATAATCTTGGAAGTTCTAAATCATCACTGAGCTTAAATATTTCATGTATTTGGTTGTTGTGAGCTTGGAAAGAATATTATAGAAAAACAACATTGACCCTAAATTTTAAAGCTCAAATTCTTGGAATCTTATTACTGTTGATCAGATTCAGGCAATATGGACTGTGGGAAAAATATGCAGAGTTATATCCTGATTCAGACTTAGTATATACAGCTGGTGTCAGTGATTACACAAAAGATTGGTTCTTTGCTCATGTTAATCGGTAAGTATGTCCAATATATGGTGTAGGTGTAAGTTTAGAAGGTTTTTCCTTTTTAATTTTATTTAAGGAAAATTATTATAAATAGAGAAAATATCCAACTATTTATAAATATAGAAAAATTTCACTTCTATCGCTCGCTCAAGCGATAGAAATCTATCGTCGATAGAAATTGAAAATTTTCTATATTTGTAAATAGTTTGACTCATTTTTCTATATTTGAGAACAACCCTTTCTTTAATGACAGAACGAGAAATGATGGATCTCATTTAGCAACCACTTGGGAAATCAAGTTTACACTTGATAATGTTATAAATAAGAGCGGCACTTATACATTGCGAGTGGCTCTGGCCACTGCCAATGTTGCTGAACTGCAGGTACTCATCCTATATTACACTGTTTAACTTTATGAGACATTTGCATTGATAAGATTTTAAGCATTAAAAAAAATCAATAGTACTTCTAGAACAAACAATTAATCAATACAACGATTTTTGTGAAATTTAAGGACAAAACTTGTGATTTAATCAAATAATGAACTTCCATTATTGATGAGCTATTATCAATAATCATTTTTAACTTTTTAATATTATTGATTCTTTTGATTCCGTATTATAGATCTCTACTAGTGAATTACAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTATTTGGTCCTTAAGTTTTGGGTCTAATTTTCTATTTGGTCCCTAGCTTTCAAAATGTTACATTTTTAGTTATTAAGTTTTGAGTTTGATTTCAATTTAGTCATTAGATTTTAAAATGTTACAATTTTACATTTGAGATTCAAATTTTGTTTCAATTTAGTCCTTAGATTTCAAGATTTATACTTTTAACCTCAAATTTCCACTAAAAAGAAGAATTAACATAAATGCATATTATTTTAATTTTTTTTTTTGTAAAGAAATATTGACAATGTGATTTTTTTTTTCTTTTTTCCTAAAAAAAAAAAGATCCAGGTAAATAATCCAGAAGCAAAACCTAGTTTATTTACAACAGGAGTGATTGGGCATGACAATGCAATTGCTAGGCATGGAATTCATGGACTGTATCGACTTTATAGTGTGGAAATATTGGGATCTCTACTAGTGGAAGGAGAGAACACCATCTTCCTTTCACAAATTATAAGCAGTTCATCTTTCAACGGCCTCATGTATGATTATATTCGCCTCGAAGGCCCCCCATCCGACGCCTCTACTAATTCATAA

mRNA sequence

ATGGCAAATGAGATACTTCAATTGGATATTCAGGATCATCATGTAGTTATGGACAATGGTATATTGCAATTGACACTGTCCAAGCCAGATGGAAATGTAACTGGGATAAAATACAACAGCATTGACAATGTTTTAGAAGTTGATGATAAGGAAGCTCATAGAGGGTACTGGGACCTCGTGTGGAACTCTGGGACTCCGGGAACCACGGGAACGTTCGATGAGATGAGAGGAAAAGAATTTGAAGTGATTGTGCAAAATGAGGAACAAATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCTGTTCCCTTAAACATAGACAAAAGGTTCATAATGCTTCGTAACTCCTCTGGATTCTACTCCTATGCCATCTATGAGCACATGAGGGAATGGCCACCATTCAACCTTCCTCAAACAAGGATTGTGTTTAAGCTAAGAAAGGACAAGTTTCATTATATGGCCATAGCAGATGACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTACGTCACAGAGGAAAACCTTTGGATTTTCCAGAGGCTGTATTGCTAGTCAACCCTAAAGAACCTGAGTTCAAGGGAGAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTCCGGTGGGTTTCTGGCAAATCACTCCAAGCAGTGAGTTCAGATCAGGCGGACCCCTCAAACAGAACCTTACCTCTCACGTGGGCCCATATGCTCTTGCTATGTTTCTTAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAGCTCAGTCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTTTCTATCTAAATTCTGTTTCTGATGGAGGTGATCCACTTTCTTTGTGGGAAGATGCTAAAACTCAGGAAAGTTTAGGACTAAATAATTTGCAATACATTAAACTTGTATGCAGATATGTTAACCAAGAGCAGACGCCGGCAACTGACGCGTATGTGGGACTGGCAGCACCAGGAGATGCTGGATCATGGCAAACTGAAAACAAGGGTTACCAGTTTTGGACCAAAACAGATGCAGATGGCTCGTTTAACATTGAACACGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTATGATGTTGTCATTAGTGTCTCTGCAGGTAGTGATATGGATGTAGGTGATCTTGTTTATGAGCCGCCTAGAAACGGGCCCACATTGTGGGAAATAGGCATTCCCGATCGCTCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAAGTACATTAACAAATTGTATGTCAATCATCCAGATAGATTCAGGCAATATGGACTGTGGGAAAAATATGCAGAGTTATATCCTGATTCAGACTTAGTATATACAGCTGGTGTCAGTGATTACACAAAAGATTGGTTCTTTGCTCATGTTAATCGAACGAGAAATGATGGATCTCATTTAGCAACCACTTGGGAAATCAAGTTTACACTTGATAATGTTATAAATAAGAGCGGCACTTATACATTGCGAGTGGCTCTGGCCACTGCCAATGTTGCTGAACTGCAGGTAAATAATCCAGAAGCAAAACCTAGTTTATTTACAACAGGAGTGATTGGGCATGACAATGCAATTGCTAGGCATGGAATTCATGGACTGTATCGACTTTATAGTGTGGAAATATTGGGATCTCTACTAGTGGAAGGAGAGAACACCATCTTCCTTTCACAAATTATAAGCAGTTCATCTTTCAACGGCCTCATGTATGATTATATTCGCCTCGAAGGCCCCCCATCCGACGCCTCTACTAATTCATAA

Coding sequence (CDS)

ATGGCAAATGAGATACTTCAATTGGATATTCAGGATCATCATGTAGTTATGGACAATGGTATATTGCAATTGACACTGTCCAAGCCAGATGGAAATGTAACTGGGATAAAATACAACAGCATTGACAATGTTTTAGAAGTTGATGATAAGGAAGCTCATAGAGGGTACTGGGACCTCGTGTGGAACTCTGGGACTCCGGGAACCACGGGAACGTTCGATGAGATGAGAGGAAAAGAATTTGAAGTGATTGTGCAAAATGAGGAACAAATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCTGTTCCCTTAAACATAGACAAAAGGTTCATAATGCTTCGTAACTCCTCTGGATTCTACTCCTATGCCATCTATGAGCACATGAGGGAATGGCCACCATTCAACCTTCCTCAAACAAGGATTGTGTTTAAGCTAAGAAAGGACAAGTTTCATTATATGGCCATAGCAGATGACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTACGTCACAGAGGAAAACCTTTGGATTTTCCAGAGGCTGTATTGCTAGTCAACCCTAAAGAACCTGAGTTCAAGGGAGAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTCCGGTGGGTTTCTGGCAAATCACTCCAAGCAGTGAGTTCAGATCAGGCGGACCCCTCAAACAGAACCTTACCTCTCACGTGGGCCCATATGCTCTTGCTATGTTTCTTAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAGCTCAGTCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTTTCTATCTAAATTCTGTTTCTGATGGAGGTGATCCACTTTCTTTGTGGGAAGATGCTAAAACTCAGGAAAGTTTAGGACTAAATAATTTGCAATACATTAAACTTGTATGCAGATATGTTAACCAAGAGCAGACGCCGGCAACTGACGCGTATGTGGGACTGGCAGCACCAGGAGATGCTGGATCATGGCAAACTGAAAACAAGGGTTACCAGTTTTGGACCAAAACAGATGCAGATGGCTCGTTTAACATTGAACACGTACGCATCGGCGACTACAATCTTTATGCTTGGGTTCCTGGCTTCATTGGTGACTACCGTTATGATGTTGTCATTAGTGTCTCTGCAGGTAGTGATATGGATGTAGGTGATCTTGTTTATGAGCCGCCTAGAAACGGGCCCACATTGTGGGAAATAGGCATTCCCGATCGCTCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAAGTACATTAACAAATTGTATGTCAATCATCCAGATAGATTCAGGCAATATGGACTGTGGGAAAAATATGCAGAGTTATATCCTGATTCAGACTTAGTATATACAGCTGGTGTCAGTGATTACACAAAAGATTGGTTCTTTGCTCATGTTAATCGAACGAGAAATGATGGATCTCATTTAGCAACCACTTGGGAAATCAAGTTTACACTTGATAATGTTATAAATAAGAGCGGCACTTATACATTGCGAGTGGCTCTGGCCACTGCCAATGTTGCTGAACTGCAGGTAAATAATCCAGAAGCAAAACCTAGTTTATTTACAACAGGAGTGATTGGGCATGACAATGCAATTGCTAGGCATGGAATTCATGGACTGTATCGACTTTATAGTGTGGAAATATTGGGATCTCTACTAGTGGAAGGAGAGAACACCATCTTCCTTTCACAAATTATAAGCAGTTCATCTTTCAACGGCCTCATGTATGATTATATTCGCCTCGAAGGCCCCCCATCCGACGCCTCTACTAATTCATAA

Protein sequence

MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQESLGLNNLQYIKLVCRYVNQEQTPATDAYVGLAAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALATANVAELQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDASTNS
Homology
BLAST of HG10013428 vs. NCBI nr
Match: XP_038899392.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 578/639 (90.45%), Postives = 600/639 (93.90%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MANEILQLDIQDHHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNY FKD+AVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYPFKDEAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEHM+EWPPFNLPQTRIVFKLRKDKFHYMAIADDR+RFMPLPEDRLRHRGKPL
Sbjct: 121 GFYSYAIYEHMKEWPPFNLPQTRIVFKLRKDKFHYMAIADDRRRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAVLLVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPKFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDG DPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGVDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LWEDAKTQ +  + +  Y                    + ++ RYVNQ++  ATDAYVGL
Sbjct: 301 LWEDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNQDKMLATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPGDAGSWQTENKGYQFWTKTDADGSF+IEH+RIGDYNLYAWVPGFIGDYRYD VISVS
Sbjct: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFSIEHIRIGDYNLYAWVPGFIGDYRYDAVISVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WEKYAELYPD+DLVYT GVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVI KSGT
Sbjct: 481 WEKYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVIEKSGT 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVNNPEAKPSLFTTGVIGHDN IARHGIHGLYRLYSV+ILGSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNTIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDASTN 618
           LVEG+NTIFLSQII++SSFNG+MYDYIRLEGPPSD ST+
Sbjct: 601 LVEGDNTIFLSQIINNSSFNGVMYDYIRLEGPPSDTSTS 639

BLAST of HG10013428 vs. NCBI nr
Match: XP_008466141.1 (PREDICTED: probable rhamnogalacturonate lyase B [Cucumis melo])

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 573/636 (90.09%), Postives = 600/636 (94.34%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MANEILQLDIQDHHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEMRGKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTSGTTGTFDEMRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LWEDAKTQ S+ + +  Y                    + ++ RY+N+E+ PATDAYVGL
Sbjct: 301 LWEDAKTQMSIEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPG+AGSWQTENKGYQFWTKTDADGSF+IEHVRIGDYNLYAWVPGFIGDYRYDV+I+VS
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
            GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDPKYINKLYVNHPDRFRQYGL
Sbjct: 421 PGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WE+YAELYPD+DLVY+ GVSDYTKDWFFAHVNR RNDGSHLATTWEIKF +DN+I+KSGT
Sbjct: 481 WERYAELYPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGT 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVNNPEAKPSLFTTG+IGHDNAIARHGIHGLYRLYSV+ILGSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDA 615
           LVEGENTIFLSQII+SSSFNG+MYDYIRLEGPPSD+
Sbjct: 601 LVEGENTIFLSQIINSSSFNGVMYDYIRLEGPPSDS 636

BLAST of HG10013428 vs. NCBI nr
Match: XP_004136251.1 (probable rhamnogalacturonate lyase B [Cucumis sativus] >KGN60274.1 hypothetical protein Csa_002470 [Cucumis sativus])

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 570/635 (89.76%), Postives = 597/635 (94.02%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MANEILQLDIQDHHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGTPGTTGTFDEMRG+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLK+SPDEPWKKVFGPVFFYLNSVSDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LWEDAKTQ S  + +  Y                    + ++ RYVN+E+ PATDAYVGL
Sbjct: 301 LWEDAKTQMSKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPGD GSWQTE KGYQFWTKTDADGSF+IEHVRIGDYNLYAWVPGFIGDYRYDV+I+VS
Sbjct: 361 AAPGDVGSWQTEKKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
           +GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDPKYINKLY+NHPDRFRQYGL
Sbjct: 421 SGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WEKYAEL+PD+DLVY+ GVSDYTKDWFFAHVNR RNDGSHLATTWEIKF LDN+I+K  +
Sbjct: 481 WEKYAELHPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVS 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSV+ILGSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSD 614
           LVEGENTIFLSQII++SSFNG+MYDYIRLEGPPSD
Sbjct: 601 LVEGENTIFLSQIINNSSFNGIMYDYIRLEGPPSD 635

BLAST of HG10013428 vs. NCBI nr
Match: XP_022136955.1 (probable rhamnogalacturonate lyase B [Momordica charantia])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 548/635 (86.30%), Postives = 587/635 (92.44%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+GKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LW+DAKTQ +  + +  Y                    + ++ RYV+Q+QTPATDAYVGL
Sbjct: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPG+AGSWQTENKGYQFWTKTDADGSF+IE+ RIGDYNLYAWVPGFIGDYRYDV++S+S
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDP YINKLYV+HPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WE+YAELYPD+DLVYT GVSDYTKDWFFAHVNRTRNDGSHL+TTW+IKFT+DNV +K+  
Sbjct: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSV++ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSD 614
           LVEGENTIFLSQIISSSSFNG+MYDYIRLE PPSD
Sbjct: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPSD 634

BLAST of HG10013428 vs. NCBI nr
Match: XP_023535230.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 538/644 (83.54%), Postives = 577/644 (89.60%), Query Frame = 0

Query: 1   MANE-ILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDL 60
           MAN+ ILQLDI DHHVVMDNGIL +TLSKPDGNVTGIKY+SIDNVLEV DKEAHRGYWDL
Sbjct: 1   MANQTILQLDIHDHHVVMDNGILAVTLSKPDGNVTGIKYSSIDNVLEVRDKEAHRGYWDL 60

Query: 61  VWNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNS 120
           VWNSGTPGTTGTFDEMRGKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNS
Sbjct: 61  VWNSGTPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNS 120

Query: 121 SGFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKP 180
           SGFYSYAIYEH+REWPPFNLPQTRIVFKLRKDKFHYMAIAD+R+R+MPLPEDR+  RGK 
Sbjct: 121 SGFYSYAIYEHLREWPPFNLPQTRIVFKLRKDKFHYMAIADNRKRWMPLPEDRVGRRGKT 180

Query: 181 LDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISM----DPPVGFWQITPSSE 240
           LDFPEAVLLV+P+E  FKGEVDDKYQYSCENKDLKVHGWISM    DPPVGFWQITPSSE
Sbjct: 181 LDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSE 240

Query: 241 FRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDG 300
           FRSGGPLKQNLTSHVGPYALAMFLSAHY GE+LVLKL+PDEPWKKVFGPVFFYLNSV +G
Sbjct: 241 FRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLKLNPDEPWKKVFGPVFFYLNSVPNG 300

Query: 301 GDPLSLWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATD 360
            DPLSLWEDAK Q +  + +  Y                    + ++ RY  QEQ PA +
Sbjct: 301 TDPLSLWEDAKIQMTKEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYAKQEQMPAAE 360

Query: 361 AYVGLAAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDV 420
           AYVGLAAPGDAGSWQTE KGYQFWTKTDA GSF+IE+VRIGDYNLYAWVPGFIGDYRYDV
Sbjct: 361 AYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDV 420

Query: 421 VISVSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRF 480
           VISVSAGSDMDVGD V+EPPRNGPTLWEIGIPDRSAAEFY+PDP+P YINKLYV+HPDRF
Sbjct: 421 VISVSAGSDMDVGDFVFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVDHPDRF 480

Query: 481 RQYGLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVI 540
           RQYGLWE+YAELYPD+DLVYT GVSDYTKDWFFAHVNRTRNDGSHL TTW+IKFTLDN I
Sbjct: 481 RQYGLWERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNI 540

Query: 541 NKSGTYTLRVALATANVAELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVE 600
           +KSG Y LRVALATANVAELQ  VNNPE + +LFTTG IGHDNAIARHGIHGLYRLYSV+
Sbjct: 541 DKSGIYKLRVALATANVAELQIRVNNPEVESALFTTGQIGHDNAIARHGIHGLYRLYSVD 600

Query: 601 ILGSLLVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDASTN 618
           I GS+LVEGENTI LSQII++SSFNG+MYDYIRLEGPPSD ST+
Sbjct: 601 ISGSVLVEGENTILLSQIINNSSFNGVMYDYIRLEGPPSDVSTH 644

BLAST of HG10013428 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 8.4e-26
Identity = 150/627 (23.92%), Postives = 248/627 (39.55%), Query Frame = 0

Query: 2   ANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVD----DKEAHRGYW 61
           A   ++L +   +  +DNG+L++     DG+   +     + +  +     D + +R ++
Sbjct: 25  ATGAVKLTLDGMNSTLDNGLLKVRFG-ADGSAKEVWKGGTNLISRLSGAARDPDKNRSFY 84

Query: 62  DLVWNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLR 121
              ++ G        +E   +  EVI Q  +Q+ L++       D+   L ++   IM R
Sbjct: 85  LDYYSGGV-------NEFVPERLEVIKQTPDQVHLAY-----IDDQNGKLRLEYHLIMTR 144

Query: 122 NSSGFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRG 181
           + SG YSY +  +     P  + + R V+  R D      + +  +R  PL  D L    
Sbjct: 145 DVSGLYSYVVAANTGS-APVTVSELRNVY--RFDATRLDTLFNSIRRGTPLLYDELEQLP 204

Query: 182 KPLDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFR 241
           K  D  E   L +       G V  KY ++   ++ +   W  M    G W +  S E+ 
Sbjct: 205 KVQD--ETWRLPD-------GSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEYY 264

Query: 242 SGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGD 301
           SG  LKQ L  H     L     +H+   D+V +      ++K++GP   Y+N  +D   
Sbjct: 265 SGDALKQELLVHQDAIILNYLTGSHFGTPDMVAQ----PGFEKLYGPWLLYINQGNDREL 324

Query: 302 PLSLWEDAKTQES----LGLNNLQYIKLVCRYVNQEQTPATDAYVGLAAPGDAGSWQTEN 361
              +   A+ + +      L++ +Y +       + +T A  A V L +   A ++  + 
Sbjct: 325 VADVSRRAEHERASWPYRWLDDARYPRQRATVSGRLRTEAPHATVVLNS--SAENFDIQT 384

Query: 362 KGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPG--FIGDYRYDVVISVSAGSDMDVGDLV 421
            GY F  +T+ DG F++ +V  G+Y L A+  G   IG      V     G    +G + 
Sbjct: 385 TGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQTV--RVEGKKTRLGQID 444

Query: 422 YEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYPDS 481
              P   P  W IG  DR A EF                  D+ RQY  W+        +
Sbjct: 445 ARQP--APLAWAIGQADRRADEF---------------RFGDKPRQY-RWQTEV----PA 504

Query: 482 DLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALATA- 541
           DL +  G S   KDW++A              +W I F   N       YTL +A+A A 
Sbjct: 505 DLTFEIGKSRERKDWYYAQTQ---------PGSWHILF---NTRTPEQPYTLNIAIAAAS 564

Query: 542 -NVAELQVNNPE----AKPSLFTTGVIGHDNAIARHGIH-GLYRLYSVEILGSLLVEGEN 601
            N      ++P+        L TT    +D +I R  +  G Y    + +    L +G N
Sbjct: 565 NNGMTTPASSPQLAVKLNGQLLTTLKYDNDKSIYRGAMQSGRYHEAHIPLPAGALQQGGN 577

Query: 602 TIFLSQIISSSSFNGLMYDYIRLEGPP 612
            I L  +        +MYD I L   P
Sbjct: 625 RITLELLGGM-----VMYDAITLTETP 577

BLAST of HG10013428 vs. ExPASy Swiss-Prot
Match: Q0C7K7 (Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rglB PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.4e-19
Identity = 116/451 (25.72%), Postives = 184/451 (40.80%), Query Frame = 0

Query: 120 SGFYSYAIYEHMREWPPF--NLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRG 179
           +G + ++   +  E  PF  NL + R +F+   D + ++  ++   +  PLP D     G
Sbjct: 130 TGLHMFSRLAYYNETTPFLRNLQEFRTLFRPNTDLWTHLTSSE--LQTAPLPSDEA--VG 189

Query: 180 KPLDFPEAVLLVN--PKEPEFK--GEVDDKYQYSCENKDLKVHGW-----ISMDPPVGFW 239
           K +   +A    N  P +  +    E   KY +S   +D  VHG       S     G W
Sbjct: 190 KQVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNAWRDNNVHGLYADGSTSNGTTFGAW 249

Query: 240 QITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFY 299
            +  + +   GGPL  +LT     Y    ++ +++ GE      +    + + FGP F+ 
Sbjct: 250 LVMNTKDTYYGGPLHSDLTVDGIVY---NYIVSNHHGEGTP---NITNGFDRTFGPQFYL 309

Query: 300 LNSVSDGGDPLSLWEDAKTQESLG--LNNLQY----IKLVCRYVNQEQTPATDAYVGLAA 359
            N    GG   SL E     ESL     N+++     K V  YV   +  +    + L  
Sbjct: 310 FN----GGGSSSLNELRSEAESLADPSWNVEFYDSIAKHVVGYVPSSKRGSVQGQIKLPR 369

Query: 360 PGDA--------GSWQTEN----KGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGD 419
                       G +  +N    + YQ+W + DA+G F ++HV  G Y L  +  G  GD
Sbjct: 370 GATRPIAILTVDGQYFQDNSVDPRSYQYWVEMDANGKFQLDHVVEGKYRLTVYADGIFGD 429

Query: 420 YRYDVVISVSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEF-YVPDPDPKYINKLYV 479
           Y  D V  V       + D  ++P   G  +W +G PD+S+ EF +    DP      + 
Sbjct: 430 YVRDGV-QVRGRKTTRIND-SWQPESAGVEVWRLGTPDKSSGEFLHGVARDP-----THP 489

Query: 480 NHPDRFRQYGLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVN----RTRNDGSHLATT- 534
            HP  +  Y     + + +P+  + YT G SD   D+   H +       N      TT 
Sbjct: 490 LHPPEYLIYWGAYDWQQDFPNG-VNYTIGSSDPATDFNTVHWSVFGPTPDNPDVEYDTTH 549

BLAST of HG10013428 vs. ExPASy Swiss-Prot
Match: B8N5T6 (Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglB PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.9e-18
Identity = 110/447 (24.61%), Postives = 182/447 (40.72%), Query Frame = 0

Query: 120 SGFYSYAIYEHMREWPPF--NLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRG 179
           +G ++++   +  E  PF  NL + R +F+   + + ++  ++   +  PLP        
Sbjct: 131 TGLHTFSRLAYYNETTPFLRNLQEFRTLFRPNTELWTHLTSSE--AQTAPLPSKEAIANE 190

Query: 180 KPLDFPEAVLLVNPKEPEFK--GEVDDKYQYSCENKDLKVHGWI-----SMDPPVGFWQI 239
             +          P +  +    E   KY +S   +D  VHG       S     G W +
Sbjct: 191 VVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNLWRDNSVHGLYADGTNSNGTTYGAWLV 250

Query: 240 TPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLN 299
             + +   GGPL  +LT     Y    +L +++ GE      +    + + FGP ++  N
Sbjct: 251 MNTKDTYYGGPLHSDLTVDGIVY---NYLVSNHHGEGTP---NITNGFDRTFGPQYYLFN 310

Query: 300 SVSDGGDPL-SLWEDAKTQESLGLNNLQY---IKLVCRYVNQEQTPATDAYVGLAAPG-- 359
                   L  L  +A+T    G N   Y    K V  Y    +  +    V L      
Sbjct: 311 GGKGSKSSLEDLRSEAETLADPGWNADFYDSIAKHVIGYAPSSKRGSVQGQVKLPKGSTR 370

Query: 360 -------DAGSWQ---TENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYD 419
                  D   +Q    E   +Q+W +   DG+F ++HV+ G Y L  +  G  GD+ +D
Sbjct: 371 PIAILTVDGQYFQDNSVEASSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIFGDFVHD 430

Query: 420 VVISVSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPD-PDPKYINKLYVNHPD 479
            V  V AG    V +  +E    G  +W +G PD+S+ EF   D PDP +     ++ P 
Sbjct: 431 GV-EVQAGKVTKVQE-TWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTH----PLHPPQ 490

Query: 480 RFRQYGLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVN----RTRNDGSHLATT--WEI 534
            F  +G ++ + + +P+  + YT G SD   D+   H +       N      TT  W I
Sbjct: 491 HFIYWGAYD-WQQDFPNG-VNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTI 550

BLAST of HG10013428 vs. ExPASy Swiss-Prot
Match: B8NCU7 (Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglA PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.1e-08
Identity = 74/264 (28.03%), Postives = 112/264 (42.42%), Query Frame = 0

Query: 353 NKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRY-DVVISVSAGS--DMDVGD 412
           N   Q+WT T +DGSF    ++ GDY +      + G+Y+  +  +SV+AGS    D+  
Sbjct: 299 NADAQYWTYTSSDGSFTSPAMKPGDYTMVY----YQGEYKVAETSVSVTAGSSTSKDISG 358

Query: 413 LVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYP 472
            V      G T+++IG  D +   F   +      N+L + HP   R    W        
Sbjct: 359 FV----ETGDTIFKIGDWDGTPTGFRNAE------NQLRM-HPSDSRM-SSW-------- 418

Query: 473 DSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALA- 532
              L YT G S+ T D+  A      +  +       IKFT  +   ++G  TLR+    
Sbjct: 419 -GPLTYTVGSSELT-DFPMAAFKGVNDPVT-------IKFTATSA--QTGAATLRIGTTL 478

Query: 533 -------TANVAELQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVE 592
                   A + + + + P A  +L + GV        R    GL  +Y V I    +VE
Sbjct: 479 SFAGGRPQATINDYEGSAPSAPTNLNSRGV-------TRGAYRGLGEVYDVNIPSGTIVE 520

Query: 593 GENTIFLSQIISSSSFNGLMYDYI 606
           GENTI +S I  SS    L  ++I
Sbjct: 539 GENTITISVISGSSGDEFLAPNFI 520

BLAST of HG10013428 vs. ExPASy Swiss-Prot
Match: Q2U0Q1 (Probable rhamnogalacturonate lyase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglA PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 7.9e-08
Identity = 71/264 (26.89%), Postives = 110/264 (41.67%), Query Frame = 0

Query: 353 NKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRY---DVVISVSAGSDMDVGD 412
           N   Q+WT T +DGSF    ++ GDY +      + G+Y+     V ++V + +  D+  
Sbjct: 299 NADAQYWTYTSSDGSFTSPAMKPGDYTMVY----YQGEYKVAETSVSVTVGSSTSKDISG 358

Query: 413 LVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYP 472
            V      G T+++IG  D +   F   +      N+L + HP   R    W        
Sbjct: 359 SV----ETGDTIFKIGDWDGTPTGFRNAE------NQLRM-HPSDSRM-SSW-------- 418

Query: 473 DSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALA- 532
              L YT G S+ T D+  A      +  +       IKFT  +   ++G  TLR+    
Sbjct: 419 -GPLTYTVGSSELT-DFPMAAFKGVNDPVT-------IKFTATSA--QTGAATLRIGTTL 478

Query: 533 -------TANVAELQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVE 592
                   A + + + + P A  +L + GV        R    GL  +Y V I    +VE
Sbjct: 479 SFAGGRPQATINDYEGSAPSAPTNLNSRGV-------TRGAYRGLGEVYDVNIPSGTIVE 520

Query: 593 GENTIFLSQIISSSSFNGLMYDYI 606
           GENTI +S I  SS    L  ++I
Sbjct: 539 GENTITISVISGSSGDEFLAPNFI 520

BLAST of HG10013428 vs. ExPASy TrEMBL
Match: A0A1S3CQT8 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1)

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 573/636 (90.09%), Postives = 600/636 (94.34%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MANEILQLDIQDHHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEMRGKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTSGTTGTFDEMRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LWEDAKTQ S+ + +  Y                    + ++ RY+N+E+ PATDAYVGL
Sbjct: 301 LWEDAKTQMSIEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPG+AGSWQTENKGYQFWTKTDADGSF+IEHVRIGDYNLYAWVPGFIGDYRYDV+I+VS
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
            GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDPKYINKLYVNHPDRFRQYGL
Sbjct: 421 PGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WE+YAELYPD+DLVY+ GVSDYTKDWFFAHVNR RNDGSHLATTWEIKF +DN+I+KSGT
Sbjct: 481 WERYAELYPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGT 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVNNPEAKPSLFTTG+IGHDNAIARHGIHGLYRLYSV+ILGSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDA 615
           LVEGENTIFLSQII+SSSFNG+MYDYIRLEGPPSD+
Sbjct: 601 LVEGENTIFLSQIINSSSFNGVMYDYIRLEGPPSDS 636

BLAST of HG10013428 vs. ExPASy TrEMBL
Match: A0A0A0LGY0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 570/635 (89.76%), Postives = 597/635 (94.02%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MANEILQLDIQDHHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV
Sbjct: 1   MANEILQLDIQDHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGTPGTTGTFDEMRG+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTPGTTGTFDEMRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL
Sbjct: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLK+SPDEPWKKVFGPVFFYLNSVSDGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LWEDAKTQ S  + +  Y                    + ++ RYVN+E+ PATDAYVGL
Sbjct: 301 LWEDAKTQMSKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPGD GSWQTE KGYQFWTKTDADGSF+IEHVRIGDYNLYAWVPGFIGDYRYDV+I+VS
Sbjct: 361 AAPGDVGSWQTEKKGYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
           +GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFY+PDPDPKYINKLY+NHPDRFRQYGL
Sbjct: 421 SGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WEKYAEL+PD+DLVY+ GVSDYTKDWFFAHVNR RNDGSHLATTWEIKF LDN+I+K  +
Sbjct: 481 WEKYAELHPDADLVYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVS 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSV+ILGSL
Sbjct: 541 YTLRVALATANVAELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSD 614
           LVEGENTIFLSQII++SSFNG+MYDYIRLEGPPSD
Sbjct: 601 LVEGENTIFLSQIINNSSFNGIMYDYIRLEGPPSD 635

BLAST of HG10013428 vs. ExPASy TrEMBL
Match: A0A6J1C5C7 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 548/635 (86.30%), Postives = 587/635 (92.44%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           MA  ILQL+IQ+HHVVMDNGILQ+TLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLV
Sbjct: 1   MAGAILQLNIQNHHVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLV 60

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120
           WNSGT GTTGTFDEM+GKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS
Sbjct: 61  WNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSS 120

Query: 121 GFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPL 180
           GFYSYAIYEH++EWPPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK L
Sbjct: 121 GFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTL 180

Query: 181 DFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGG 240
           DFPEAV LVNPKEP+FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGG
Sbjct: 181 DFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGG 240

Query: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 300
           PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLS
Sbjct: 241 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLS 300

Query: 301 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 360
           LW+DAKTQ +  + +  Y                    + ++ RYV+Q+QTPATDAYVGL
Sbjct: 301 LWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGL 360

Query: 361 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 420
           AAPG+AGSWQTENKGYQFWTKTDADGSF+IE+ RIGDYNLYAWVPGFIGDYRYDV++S+S
Sbjct: 361 AAPGEAGSWQTENKGYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSIS 420

Query: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 480
           AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDP YINKLYV+HPDRFRQYGL
Sbjct: 421 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDRFRQYGL 480

Query: 481 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 540
           WE+YAELYPD+DLVYT GVSDYTKDWFFAHVNRTRNDGSHL+TTW+IKFT+DNV +K+  
Sbjct: 481 WERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNV-DKNDA 540

Query: 541 YTLRVALATANVAEL--QVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 600
           YTLRVALATANVAEL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSV++ GSL
Sbjct: 541 YTLRVALATANVAELQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSL 600

Query: 601 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSD 614
           LVEGENTIFLSQIISSSSFNG+MYDYIRLE PPSD
Sbjct: 601 LVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPSD 634

BLAST of HG10013428 vs. ExPASy TrEMBL
Match: A0A6J1FDW4 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 SV=1)

HSP 1 Score: 1107.8 bits (2864), Expect = 0.0e+00
Identity = 534/640 (83.44%), Postives = 572/640 (89.38%), Query Frame = 0

Query: 5   ILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSG 64
           ILQLDI DHHVVMDNGIL +TLSKP GNVTGIKY+SIDNVLEV DKEAHRGYWDLVWNSG
Sbjct: 6   ILQLDIHDHHVVMDNGILAVTLSKPYGNVTGIKYSSIDNVLEVRDKEAHRGYWDLVWNSG 65

Query: 65  TPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYS 124
           TPGTTGTFDEMRGKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFYS
Sbjct: 66  TPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFYS 125

Query: 125 YAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPE 184
           YAIYEH+REWPPFNLPQTRIVFKLRKDKFHYMAIAD+R+R MPLP+DR+  RGK LDFPE
Sbjct: 126 YAIYEHLREWPPFNLPQTRIVFKLRKDKFHYMAIADNRKRSMPLPDDRVGGRGKTLDFPE 185

Query: 185 AVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISM----DPPVGFWQITPSSEFRSGG 244
           AVLLV+P+E  FKGEVDDKYQYSCENKDLKVHGWISM    DPPVGFWQITPSSEFRSGG
Sbjct: 186 AVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSGG 245

Query: 245 PLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLS 304
           PLKQNLTSHVGPYALAMFLSAHY GE+LVLKL+ DEPWKKVFGPVFFYLNSV +G DPL 
Sbjct: 246 PLKQNLTSHVGPYALAMFLSAHYGGEELVLKLNADEPWKKVFGPVFFYLNSVPNGRDPLC 305

Query: 305 LWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGL 364
           LWEDAK Q +  + +  Y                    + ++  YVNQEQ PA +AYVGL
Sbjct: 306 LWEDAKIQMTKEVQSWPYSFPASQNFPPPNQRGRLTGNLYVLDTYVNQEQMPAAEAYVGL 365

Query: 365 AAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVS 424
           AAPGDAGSWQTE KGYQFWTKTDA GSF+IE+VRIGDYNLYAWVPGFIGDYRYDVVISVS
Sbjct: 366 AAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVS 425

Query: 425 AGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGL 484
           AGSDMDVGDLV+EPPRNGPTLWEIGIPDRSAAEFY+PDP+P YINKLYV+HPDRFRQYGL
Sbjct: 426 AGSDMDVGDLVFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVHHPDRFRQYGL 485

Query: 485 WEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGT 544
           WE+YAELYPD+DLVYT GVSDYTKDWFFAHVNRTRNDGSHL TTW+IKFTLDN I+KSG 
Sbjct: 486 WERYAELYPDTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGI 545

Query: 545 YTLRVALATANVAELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSL 604
           Y LRVALATANVAELQ  VNNPE +P+LFTTG IGHDNAIARHGIHGLYRLYSV++  S+
Sbjct: 546 YKLRVALATANVAELQIRVNNPEVEPALFTTGQIGHDNAIARHGIHGLYRLYSVDMSRSV 605

Query: 605 LVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDASTNS 619
           LVEGENTIFLSQII++SSFNG+MYDYIRLEGPPSD ST S
Sbjct: 606 LVEGENTIFLSQIINTSSFNGVMYDYIRLEGPPSDVSTRS 645

BLAST of HG10013428 vs. ExPASy TrEMBL
Match: A0A6J1IFC5 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 528/644 (81.99%), Postives = 574/644 (89.13%), Query Frame = 0

Query: 1   MANE-ILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDL 60
           MAN+  LQLDI DHHVVMDNGIL +TLSKPDGNVTGIKY++IDN+LEV DKEAHRGYWDL
Sbjct: 1   MANKATLQLDIHDHHVVMDNGILAVTLSKPDGNVTGIKYSNIDNILEVRDKEAHRGYWDL 60

Query: 61  VWNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNS 120
           VWNSGTPGTTGTFDEMRGKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNS
Sbjct: 61  VWNSGTPGTTGTFDEMRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNS 120

Query: 121 SGFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKP 180
           SGFYSYAIYEH+REWPPFNLPQTRIVFKL KDKFHYMAIAD+R+R MPLP+DR+  RGK 
Sbjct: 121 SGFYSYAIYEHLREWPPFNLPQTRIVFKLSKDKFHYMAIADNRKRSMPLPDDRVGRRGKT 180

Query: 181 LDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISM----DPPVGFWQITPSSE 240
           LDFPEAVLLV+P+E  FKGEVDDKYQYSCENKDLKVHGWISM    DPPVGFWQITPSSE
Sbjct: 181 LDFPEAVLLVHPREAAFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSE 240

Query: 241 FRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDG 300
           FRSGGPLKQNLTSHVGPYALAMFLSAHY GE+LVLK++PDEPWKKVFGPVFFYLNSV +G
Sbjct: 241 FRSGGPLKQNLTSHVGPYALAMFLSAHYGGEELVLKMNPDEPWKKVFGPVFFYLNSVPNG 300

Query: 301 GDPLSLWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATD 360
            DPLSLWEDAKTQ +  + +  Y                    + ++ RYVNQEQ PA +
Sbjct: 301 TDPLSLWEDAKTQMTKEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYVNQEQMPAAE 360

Query: 361 AYVGLAAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDV 420
           AYVGLAAPGDAGSWQTE KGYQFWTKTDA GSF+IE+VRIGDYNLYAWVPGFIGDY YDV
Sbjct: 361 AYVGLAAPGDAGSWQTETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYHYDV 420

Query: 421 VISVSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRF 480
           VISVSAGSD+D+GDLV+EPPRNGPT+WEIGIPDRSA EFY+PDP+P YINKLYV+HPDRF
Sbjct: 421 VISVSAGSDIDIGDLVFEPPRNGPTVWEIGIPDRSANEFYIPDPNPNYINKLYVDHPDRF 480

Query: 481 RQYGLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVI 540
           RQYGLWE+YAELYPD+DLVYT GVSDY KDWFFAHVNRTRNDGSHL TTW+IKFTLDN +
Sbjct: 481 RQYGLWERYAELYPDTDLVYTVGVSDYPKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNV 540

Query: 541 NKSGTYTLRVALATANVAELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVE 600
           +KSG Y LRVALATANVAELQ  VNN E + +LFTTG IGHDNAIARHGIHGLYRLYSV+
Sbjct: 541 DKSGIYKLRVALATANVAELQIRVNNLEVEHALFTTGQIGHDNAIARHGIHGLYRLYSVD 600

Query: 601 ILGSLLVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPSDASTN 618
           + GS+LVEGENTIFLSQII+ SSFNG+MYDYIRLEGPPSD ST+
Sbjct: 601 MSGSVLVEGENTIFLSQIINDSSFNGVMYDYIRLEGPPSDVSTH 644

BLAST of HG10013428 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 833.9 bits (2153), Expect = 8.1e-242
Identity = 398/638 (62.38%), Postives = 499/638 (78.21%), Query Frame = 0

Query: 6   LQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVW-NSG 65
           +QLD+Q+ HVVM NG +++T+SKPDG VTGI Y  +DN+LE  +++ +RGYWDLVW + G
Sbjct: 7   VQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEG 66

Query: 66  TPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGF 125
           TPGTTG  + ++G  FEV+V+NEE +E+SF R +  S +D   P+N+DKRFIM ++ +GF
Sbjct: 67  TPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGF 126

Query: 126 YSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDF 185
           YSYAI+EH+ EWP FNLPQTRIV+KLRKDKF YMAIAD+RQR MPLPEDRL  RG+PL +
Sbjct: 127 YSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAY 186

Query: 186 PEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPL 245
           PEAVLLV+P E EFKGEVDDKY+YS ENKDLKVHGWIS +  +G WQI PS+EFRSGG  
Sbjct: 187 PEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLS 246

Query: 246 KQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSD-GGDPLSL 305
           KQNLTSHVGP +LAMFLSAHY+GED+V+K+   + WKKVFGPVF YLN + D   DPLSL
Sbjct: 247 KQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSL 306

Query: 306 WEDAKTQESLGLNNLQY-------------------IKLVC-RYVNQEQTPATDAYVGLA 365
           W+DAK Q    + +  Y                     LVC ++++ +  PA  A+VGLA
Sbjct: 307 WQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLA 366

Query: 366 APGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVSA 425
            PG+ GSWQ E+KGYQFWT+ D+DG F I  +R G+YNL  +V G+IGDY+Y+ +I+++A
Sbjct: 367 PPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITA 426

Query: 426 GSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLW 485
           G D+DVG++VYEPPR+GPT+WEIGIPDRSAAEF+VPDP+PKYINKLY+ HPDRFRQYGLW
Sbjct: 427 GCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLW 486

Query: 486 EKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTY 545
           E+Y ELYP  DLV+T GVSDY KDWFFAHV R   D ++  TTW+IKF L+NV  KS TY
Sbjct: 487 ERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENV-QKSCTY 546

Query: 546 TLRVALATANVAELQV----NNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGS 605
            +R+ALATANVAELQV    ++ E    +FTTGVIGHDNAIARHGIHG+YRLY+V++   
Sbjct: 547 KIRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSE 606

Query: 606 LLVEGENTIFLSQIISSS-SFNGLMYDYIRLEGPPSDA 615
            LVEG+NT+FL+Q ++++ +FNGLMYDYIRLEGPP D+
Sbjct: 607 KLVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPPLDS 643

BLAST of HG10013428 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 816.6 bits (2108), Expect = 1.3e-236
Identity = 399/620 (64.35%), Postives = 478/620 (77.10%), Query Frame = 0

Query: 17  MDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDEMR 76
           MDNGI ++TLSKPDG VTGI+YN IDN+LEV ++E +RGYWDLVW  G  GT G FD ++
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVW--GGSGTAGGFDVIK 60

Query: 77  GKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREW 136
           G  FEVIV+NEEQIELSF R +  S + KAVPLNIDKRF+ML  SSGFY+YAIYEH++EW
Sbjct: 61  GSNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEW 120

Query: 137 PPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 196
           P F+L +TRI FKLRK+KFHYMA+ DDRQRFMPLP+DRL  RG+ L +PEAVLLVNP E 
Sbjct: 121 PAFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLES 180

Query: 197 EFKGEVDDKYQYSCENKDLKVHGWISMD-PPVGFWQITPSSEFRSGGPLKQNLTSHVGPY 256
           +FKGEVDDKYQYSCENKD+ VHGWI  + P VGFW ITPS E+R+GGP KQNLTSHVGP 
Sbjct: 181 QFKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPT 240

Query: 257 ALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLN-SVSDGGDPLSLWEDAKTQESLG 316
           ALA+F+SAHY+GEDLV K S  E WKKVFGPVF YLN S  D  DPL LW+DAK+Q ++ 
Sbjct: 241 ALAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVE 300

Query: 317 LNNLQY--------------------IKLVCRYVNQEQTPATDAYVGLAAPGDAGSWQTE 376
             +  Y                    + +  RYV+++   A   YVGLA PG AGSWQ E
Sbjct: 301 AESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRE 360

Query: 377 NKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVY 436
            K YQFWT+TD +G F I  +R G YNLYAW+PGFIGDY+YD VI++++G  + V DLVY
Sbjct: 361 CKEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVY 420

Query: 437 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYPDSD 496
           +PPRNG TLWEIG PDRSAAEFYVPDP+PKYIN LY NHPDRFRQYGLWE+YAELYPD D
Sbjct: 421 QPPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKD 480

Query: 497 LVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALATANV 556
           LVY  G SDY KDWF+A V R +++ ++  TTW+IKF L N I+K+ +YTLRVA+A+A  
Sbjct: 481 LVYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKN-IDKNHSYTLRVAIASATF 540

Query: 557 AELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVEGENTIFLSQ 611
           +ELQ  VNN  A P +FT+G+IG DN+IARHGIHGLY L++VE+ GS L+EGENT+FL+Q
Sbjct: 541 SELQIRVNNANASP-MFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQ 600

BLAST of HG10013428 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-229
Identity = 378/636 (59.43%), Postives = 470/636 (73.90%), Query Frame = 0

Query: 1   MANEILQLDIQDHHVVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLV 60
           M++  + L + D +VVMDNGILQ+TLSKP G +TGI+YN IDNVLEV +KE +RGYWDL 
Sbjct: 44  MSSHGVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLH 103

Query: 61  WNSGTPGTTGTFDEMRGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRN 120
           WN   PG  G FD + G  F VIV+ EEQ+E+SF R +  S + K +PLNIDKRFIMLR 
Sbjct: 104 WNE--PGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRG 163

Query: 121 SSGFYSYAIYEHMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGK 180
           SSG YSY IYEH+++WP F L +TRI FKLRKDKFHYMA+ADDR+R MP P+D  + R +
Sbjct: 164 SSGVYSYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQ 223

Query: 181 PLDFPEAVLLVNPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRS 240
            LD+ EA LL  P +P  +GEVDDKYQYSCENKDL+VHGWIS DPPVGFWQITPS+EFRS
Sbjct: 224 TLDYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRS 283

Query: 241 GGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDP 300
           GGPLKQNLTSHVGP  LA+F S HY+G+ ++ +    EPWKKV+GPVF YLNS ++G DP
Sbjct: 284 GGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDP 343

Query: 301 LSLWEDAKTQESLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYV 360
           L LW+DAK +    +    Y                    + +  R++N +   A  AYV
Sbjct: 344 LCLWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYV 403

Query: 361 GLAAPGDAGSWQTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVIS 420
           GLA PGD+GSWQ E KGYQFW   D  G F+I +VR G+YNLYAWVP FIGDY    ++ 
Sbjct: 404 GLAPPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVR 463

Query: 421 VSAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQY 480
           V++G  +++GD+VYEPPR+GPTLWEIGIPDR A+EF++PDPDP  +N++ V+H DRFRQY
Sbjct: 464 VTSGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQY 523

Query: 481 GLWEKYAELYPDSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKS 540
           GLW+KY ++YP+ DLVYT GVSDY +DWFFAHV R + D  H  TTW+I F L+N I++ 
Sbjct: 524 GLWKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRKKGD-VHEGTTWQIIFNLEN-IDQK 583

Query: 541 GTYTLRVALATANVAELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILG 600
             Y LRVA+A+A +AELQ  +N+ EA   LFTTG+IG DN+IARHGIHG+Y LY+V I G
Sbjct: 584 ANYKLRVAIASATLAELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPG 643

Query: 601 SLLVEGENTIFLSQIISSSSFNGLMYDYIRLEGPPS 613
           + LV+G+NTIFL Q   +  F G+MYDYIRLEGPPS
Sbjct: 644 NRLVQGDNTIFLKQPRCNGPFQGIMYDYIRLEGPPS 675

BLAST of HG10013428 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.5e-216
Identity = 365/626 (58.31%), Postives = 450/626 (71.88%), Query Frame = 0

Query: 17  MDNGILQLTLSKPDGNVTGIKYNSIDNVLE-VDDKEAHRGYWDLVWNSGTPG-----TTG 76
           M+N  LQLTLS P+G VTGI+YN IDNVL    +KE  RGYWD+VWN   PG     T G
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWN--FPGKKAKKTKG 60

Query: 77  TFDEMRGKEFEVIVQNEEQIELSFKRNYSFKD-KAVPLNIDKRFIMLRNSSGFYSYAIYE 136
           T D +   + EVI QN+E+IELSF R ++     AVP+NIDKRF+ML+NSSGFYSYAI+E
Sbjct: 61  TLDRIEATKMEVITQNDEKIELSFTRTWNTSSTTAVPVNIDKRFVMLQNSSGFYSYAIFE 120

Query: 137 HMREWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLV 196
            ++ WP   L   R+VFKL K KFHYMAI+DDRQR+MP+P+DR+  RG+PL +PEAV L+
Sbjct: 121 RLQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLL 180

Query: 197 NPKEPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSH 256
           +P EPEFKGEVDDKY+YS E+KD+KVHGWIS +  VGFWQITPS+EFRS GPLKQ L SH
Sbjct: 181 DPIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSH 240

Query: 257 VGPYALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQE 316
           VGP  LA+F S HY G DL++     E WKKVFGPVF YLNS   G DPL LW +AK Q 
Sbjct: 241 VGPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQT 300

Query: 317 SLGLNNLQY--------------------IKLVCRYVNQEQTPATDAYVGLAAPGDAGSW 376
            +      Y                    + +  R+++ E  PA  +YVGLAAPGD GSW
Sbjct: 301 KIEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSW 360

Query: 377 QTENKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGD 436
           Q E KGYQFW+K D +GSF+I +VR G YNLYA+ PGFIGDY  D V  +S GS + +GD
Sbjct: 361 QRECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGD 420

Query: 437 LVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPDRFRQYGLWEKYAELYP 496
           LVYEPPR+G TLWEIG+PDRSAAEFY+PDP+P ++NKLY+NH D++RQYGLWE+Y+ELYP
Sbjct: 421 LVYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYP 480

Query: 497 DSDLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVI-NKSGTYTLRVALA 556
           D D+VY   + DY+K+WFF  V R + +G +  TTW+I+F  D+ + N +G + LR+ALA
Sbjct: 481 DEDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALA 540

Query: 557 TANVAELQ--VNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVEGENTI 613
           T+NVAELQ  VN+  A P LF T  IG DN IARHGIHGLY LYSV +  + L  G NTI
Sbjct: 541 TSNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTI 600

BLAST of HG10013428 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 704.9 bits (1818), Expect = 5.7e-203
Identity = 352/620 (56.77%), Postives = 431/620 (69.52%), Query Frame = 0

Query: 15  VVMDNGILQLTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDE 74
           VV+DNGI+Q+T S P+G +TGIKY+ IDNVL  DDK   RGYWD+VW    P      D+
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVL--DDKIDDRGYWDVVWYE--PEKKQKTDK 121

Query: 75  MRGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMR 134
           + G +FE+I QNEEQIE+SF R +  S +   VPLN+DKR+I+    SG Y Y I E + 
Sbjct: 122 LEGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLE 181

Query: 135 EWPPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPK 194
            WP  ++ Q RIVFKL   KF +MAI+DDRQR MP   D  R   K L + EAVLL NP 
Sbjct: 182 GWPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMAD--RENSKSLAYKEAVLLTNPS 241

Query: 195 EPEFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGP 254
            P FKGEVDDKY YS E+KD  VHGWIS DPPVGFW ITPS EFR GGP+KQ+LTSH GP
Sbjct: 242 NPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGP 301

Query: 255 YALAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQESLG 314
             L+MF S HY+G+++ +     EPWKKVFGPV  YLNSVS     L LW DAK Q +  
Sbjct: 302 ITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAE 361

Query: 315 LNNLQYIKLVCR--------------------YVNQEQTPATDAYVGLAAPGDAGSWQTE 374
           + +  Y  +                       YV++ +     A+VGLA  G+AGSWQTE
Sbjct: 362 VKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTE 421

Query: 375 NKGYQFWTKTDADGSFNIEHVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDLVY 434
           +KGYQFWTK D  G F IE+VR G+Y+LYAW  GFIGDY+Y+  I+++ GS+M+VG LVY
Sbjct: 422 SKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVY 481

Query: 435 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNH-PDRFRQYGLWEKYAELYPDS 494
           EPPRNGPTLWEIG+PDR+A EFY+PDP P  +NKLYVN   DRFRQYGLW++YA+LYP +
Sbjct: 482 EPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQN 541

Query: 495 DLVYTAGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVINKSGTYTLRVALATAN 554
           DLVYT GVSDY  DWFFAHV R   + ++  TTW+I F L NV N+ G YTLR+ALA+A 
Sbjct: 542 DLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNV-NRIGRYTLRIALASAA 601

Query: 555 VAELQVN-NPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVEILGSLLVEGENTIFLSQ 611
            +ELQ+  N     ++FTTG IG DNAIARHGIHGLYRLYS+++ G+LL  G+NTIFL+Q
Sbjct: 602 DSELQIRINDPKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQ 661

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899392.10.0e+0090.45probable rhamnogalacturonate lyase B [Benincasa hispida][more]
XP_008466141.10.0e+0090.09PREDICTED: probable rhamnogalacturonate lyase B [Cucumis melo][more]
XP_004136251.10.0e+0089.76probable rhamnogalacturonate lyase B [Cucumis sativus] >KGN60274.1 hypothetical ... [more]
XP_022136955.10.0e+0086.30probable rhamnogalacturonate lyase B [Momordica charantia][more]
XP_023535230.10.0e+0083.54probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8RJP28.4e-2623.92Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE... [more]
Q0C7K73.4e-1925.72Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / F... [more]
B8N5T62.9e-1824.61Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 /... [more]
B8NCU72.1e-0828.03Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 /... [more]
Q2U0Q17.9e-0826.89Probable rhamnogalacturonate lyase A OS=Aspergillus oryzae (strain ATCC 42149 / ... [more]
Match NameE-valueIdentityDescription
A0A1S3CQT80.0e+0090.09Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1[more]
A0A0A0LGY00.0e+0089.76Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=... [more]
A0A6J1C5C70.0e+0086.30Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3... [more]
A0A6J1FDW40.0e+0083.44Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 ... [more]
A0A6J1IFC50.0e+0081.99Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G24430.18.1e-24262.38Rhamnogalacturonate lyase family protein [more]
AT1G09890.11.3e-23664.35Rhamnogalacturonate lyase family protein [more]
AT1G09910.11.6e-22959.43Rhamnogalacturonate lyase family protein [more]
AT1G09880.11.5e-21658.31Rhamnogalacturonate lyase family protein [more]
AT2G22620.15.7e-20356.77Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 7..200
e-value: 2.7E-86
score: 288.4
NoneNo IPR availableGENE3D2.60.40.1120coord: 328..407
e-value: 3.9E-9
score: 38.6
NoneNo IPR availableGENE3D2.60.120.260coord: 418..608
e-value: 7.9E-18
score: 66.6
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 1..616
NoneNo IPR availablePANTHERPTHR32018:SF16RHAMNOGALACTURONATE LYASE B-RELATEDcoord: 1..616
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 14..301
e-value: 1.26493E-74
score: 237.672
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 422..608
e-value: 4.10519E-53
score: 177.082
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 335..405
e-value: 1.4E-23
score: 82.5
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 328..410
e-value: 8.93869E-29
score: 107.72
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 7..271
e-value: 2.0E-8
score: 36.0
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 420..607
e-value: 2.7E-51
score: 173.9
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 417..608
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 350..405
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 13..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013428.1HG10013428.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0102210 rhamnogalacturonan endolyase activity
molecular_function GO:0003824 catalytic activity