HG10013385 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10013385
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Aminoacyl-tRNA hydrolase
Location	Chr02: 937116 .. 939722 (+)
RNA-Seq Expression	HG10013385
Synteny	HG10013385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGGTTCTTCTAATTCTTCCAGAAAGGTATTCAGTTTCATGGGTTTCTTTCTTTCTCCCTCTCTGCCCCCTCTTGTTGATGTTTGAATTAATTTCTCGTTTTCTTTCTGTATTGTTAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTGTCGGTGAGTTTCAGACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTCTTGGATTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGCTAAGAAAGGCTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCTGCTTCAAACAACGGTGATCAAGAGCTCAAATTGGTTAGTATTCGACTTTAGTAATTTTGCAGTCTGAATTGCTGGACTTCTGTAGAGTTGGGGAAATAATTTCTCTACATGTTTGTAATGGATTATGATCATTAAATTAGAATGAAAGGAAAGAACGAAACAAGAATATTTGAGTTTAGCCTCAATTGTGTACGATTAAAAGGGCATACAATAGTACATAGGGCTTTCATAGATTAGCCTTTTTACAGGTACGGGGATGAGGTTTCCTGTTTAATGGCGATGAATATGGTTCCCCACTGCTCAAATTTTGAGGCTTGAATGCTAGTAAGATAGAAGAGTTTAGGGCCTACTTGAGGTAGTTGTATAATAATATTGCGAACAAAATTAGCTTTCAAGTGAATATGCCTACTTGTCAAATCATTAAGCAGAGTTCTAGGTTCAATGCTTACATCATCTTTGCTGTTGCCATCGAATATCTGCAGTCCGTCAGGCTAGAACGTAAACAGGGGCTTGCTAAAATATGATACAGCAGTTAGACAAGTTCTCTTTACTTTGCAAACTTGGTTATATCATATATGTATAGTATATTTGTATATTTTCAAAAGGAAAAAACTTAGGTGTAGCTGGGCTGCACCCGTCTCTTGTGCAGCCCATACTTCACCGAATGAAAAATTGACATGTGTCCATTTTTATTTTTATTTTTATAAAAAATTAATTTGTGGATGAAAGAAGGGGAGCATGGTTGCACTAAGTATTGCTCTTTCCAAAAATCTTAAAATGCTGTAGTAAAGTTGTAAAAGGGCAATCCAAATTATTTGTTTCCTTGTATTTCGAGCGTTCTTTTCATTTTATCAATGAAAAGTTATGTTTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTAGAATGCTGTAATATGGCTTATATAGATTGTCCTTCCTTAAGCATGGGAACTACACTGACAGTCGCACCAAGTCCTTGTGTCATGTAACTTGTATAATGCATCTAGCTGTCAAGAACCATCTGACATACTTACTTTTTAATTGAAGGTCCTTGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCGTAAGATTTAAGATGCTGAATTTCATACTTGTTATAGTGTGCATTTGATTCTAGTTTCTAATTACTTTGGTCTGTTTATCCCTTCACGTCTTCCTTCCTTCAACGCTTGATAATTTTGCATGTTTTTCTGTCAAATTGTGAAGATGCTGCTACTGGCTTGTATGCAGAACTCATGCAAAGGTACTCCTTATACTATACTATTTCCGAATATCTGCTTTGATCAGAGCATGTGCCCAACTAATTCTTCAACCCTCTCTTTTTTCTGCACATATACCCATTTCCAATATCTAGTGCAGCCACCGAAACCTTTTGAGACAGTGGGAGCTATGTGGGCAGCCGAAAATAGTTGTTACATGCAAGAATCAACAAGAAATGTAAGCAGGTGATTCCTTTTTCCAAAAATGTAATCGTTAATAAACATGTGAAGCCTTACAGTTTTCAAAAGGCTTTGTAGGTTTGTGTTGAGGATTGCGAGGAGGGACCGAGAGCATGGGGAAAGGGTTGTTTATTGAAGTTACACTTTTGTTGTTAAATTTACTTCTTTTCTCAAATCAGGAATAAACTGAAGGATGCAGCTGAAAACATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTACAAACTTTGTTCTGCACTCGCCTACTCGGACGAATTTACAAGCATTTTGATTAATTCATGAGAAATAGTGGGGTAACCGGGTACGATAATGCTTGACATGTTGTATTATTAATGTCTATATTTTGGCATAGTCAAACGATTCTCCACATGTTATGCCAATATGCGTTGGTATTATATTGTCCTATGAATGTTAGGCATCAGAAAGTGAATGTTATGAGATCTCCGAAGTGAAAAGAATAGCTGTTTGTGGTTCAGCATTGTATAGAGTGGCTGCAAAACCCTTGGTTTGTAGTTGATGGAAAGAAGCAGTGGGCTCTTTGAAGAGCAATTTTTTCCACCCTGTTTAGTTTAGCCAAATTCGTATGCAATTATCATTTTGATGAGCTTCAAATGCCTATAATTGGATGCCTCAGGAAACATGTTTGTATTCCATCTGGCATCCTGAAATCAGTTCAATAATTTGCAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCTATGATAGAATTCTGTGGTTGCCTGGTTGA

mRNA sequence

ATGTTGGGTTCTTCTAATTCTTCCAGAAAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTGTCGGTGAGTTTCAGACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTCTTGGATTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGCTAAGAAAGGCTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCTGCTTCAAACAACGGTGATCAAGAGCTCAAATTGGTCCTTGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCTGCTACTGGCTTGTATGCAGAACTCATGCAAAGGAATAAACTGAAGGATGCAGCTGAAAACATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCTATGATAGAATTCTGTGGTTGCCTGGTTGA

Coding sequence (CDS)

ATGTTGGGTTCTTCTAATTCTTCCAGAAAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTGTCGGTGAGTTTCAGACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTCTTGGATTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGCTAAGAAAGGCTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCTGCTTCAAACAACGGTGATCAAGAGCTCAAATTGGTCCTTGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCTGCTACTGGCTTGTATGCAGAACTCATGCAAAGGAATAAACTGAAGGATGCAGCTGAAAACATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCTATGATAGAATTCTGTGGTTGCCTGGTTGA

Protein sequence

MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASLPYDRILWLPG

Homology

[Close]

BLAST of HG10013385 vs. NCBI nr
Match: KAA0038619.1 (peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa] >TYK31218.1 peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 297.4 bits (760), Expect = 7.8e-77
Identity = 158/191 (82.72%), Postives = 161/191 (84.29%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 166
                              NKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180

[Close]

BLAST of HG10013385 vs. NCBI nr
Match: XP_008466065.1 (PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 274.2 bits (700), Expect = 7.1e-70
Identity = 146/177 (82.49%), Postives = 149/177 (84.18%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. NCBI nr
Match: XP_004136220.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321.1 hypothetical protein Csa_002634 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.6e-69
Identity = 145/177 (81.92%), Postives = 149/177 (84.18%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. NCBI nr
Match: KAG7031726.1 (Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.6 bits (693), Expect = 4.6e-69
Identity = 144/182 (79.12%), Postives = 148/182 (81.32%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGNAKKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 157
                              NKLKDAAEN+GLPTFVVADAGRTQVSAGSKTVLAIGP N  
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPDNII 180

[Close]

BLAST of HG10013385 vs. NCBI nr
Match: XP_022941498.1 (peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita moschata] >XP_022981057.1 peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita maxima] >XP_023523204.1 peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >KAG6608093.1 Peptidyl-tRNA hydrolase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 270.8 bits (691), Expect = 7.9e-69
Identity = 143/177 (80.79%), Postives = 147/177 (83.05%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGNAKKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKDAAEN+GLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 8.9e-15
Identity = 56/151 (37.09%), Postives = 75/151 (49.67%), Query Frame = 0


Query: 33  GLVIGFILGLFLDLS-KPSKGNAKKGYFLPSKYQQLSPASNNGDQ-ELKLVLVVRQDLKM 92
           GL +G   G+ L  S +   G   K     +     S AS  GD  E K++LVVR DLKM
Sbjct: 18  GLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKM 77

Query: 93  GSGKIASQCAHAATGLYAELMQRN--------------------------KLKDAAENIG 152
           G GK+A+QC+HAA   Y ++ +RN                           L   A+ +G
Sbjct: 78  GKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLG 137

Query: 153 LPTFVVADAGRTQVSAGSKTVLAIGPGNASL 156
           L   ++ DAGRTQ++ GS+TVL IGPG A L
Sbjct: 138 LTVSLIQDAGRTQIAPGSQTVLGIGPGPADL 168

[Close]

BLAST of HG10013385 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.8e-14
Identity = 43/105 (40.95%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN---------------------- 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y ++ +RN                      
Sbjct: 64  EYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEYCGQPKVVVKAPDE 123

Query: 137 ----KLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASL 156
               +L   A+ +GL   ++ DAGRTQ++ GS+TVL IGPG A L
Sbjct: 124 ETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADL 168

[Close]

BLAST of HG10013385 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.3e-13
Identity = 58/185 (31.35%), Postives = 84/185 (45.41%), Query Frame = 0


Query: 23  VSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFLPSKYQQLSPASNNGDQ------ 82
           +S  P   + G V+GF++G    ++  S+G + K     S  ++ SP S + D+      
Sbjct: 10  ISSFPAAVLVGAVVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESET 69

Query: 83  --------------------ELKLVLVVRQDLKMGSGKIASQCAHAATGLY--------- 142
                               + K+VLVVR DL M  GKIA+QCAHAA   Y         
Sbjct: 70  ELLDMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPD 129

Query: 143 ----------------AELMQRNKLKDA-AENIGLPTFVVADAGRTQVSAGSKTVLAIGP 156
                           A+  +  +L  A A ++GL   V+ DAGRTQ+++GS TVL IGP
Sbjct: 130 LVRIWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGP 189

[Close]

BLAST of HG10013385 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-12
Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN---------------------- 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y +  +R+                      
Sbjct: 66  EYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDE 125

Query: 137 ----KLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASL 156
               +L   A+ +GL   ++ DAGRTQ+  GS+TVL IGPG   L
Sbjct: 126 DTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVEL 170

[Close]

BLAST of HG10013385 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-12
Identity = 45/112 (40.18%), Postives = 56/112 (50.00%), Query Frame = 0


Query: 75  DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN-------------------- 134
           +   K+VLVVR DLKMG GKIA+QC H A G Y   + R                     
Sbjct: 69  NDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVE 128

Query: 135 ------KLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASLPYDRI 161
                  +K  AE   L T ++ DAGRTQ+ A SKTVLA+GP  A+   DR+
Sbjct: 129 SEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAVGPA-AAADIDRV 179

[Close]

BLAST of HG10013385 vs. ExPASy TrEMBL
Match: A0A5D3E5Y4 (Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005040 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.8e-77
Identity = 158/191 (82.72%), Postives = 161/191 (84.29%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 166
                              NKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180

[Close]

BLAST of HG10013385 vs. ExPASy TrEMBL
Match: A0A1S3CRS9 (Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.4e-70
Identity = 146/177 (82.49%), Postives = 149/177 (84.18%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. ExPASy TrEMBL
Match: A0A0A0LJW4 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 7.7e-70
Identity = 145/177 (81.92%), Postives = 149/177 (84.18%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. ExPASy TrEMBL
Match: A0A6J1ISX6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480317 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.8e-69
Identity = 143/177 (80.79%), Postives = 147/177 (83.05%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGNAKKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKDAAEN+GLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. ExPASy TrEMBL
Match: A0A6J1FS99 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446781 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.8e-69
Identity = 143/177 (80.79%), Postives = 147/177 (83.05%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGNAKKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 152
                              NKLKDAAEN+GLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of HG10013385 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.6e-43
Identity = 98/182 (53.85%), Postives = 122/182 (67.03%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           M  SS +S + + ++ EK+ +SVSFR EN IPG+VIGFI+G+ LDLS+      K+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPASNNG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ------- 120
            SK Q+ S    NG DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQ       
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 -------------------RNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNA 156
                               NK+ +AAE++GLPTFVVADAGRT+V+AGS+TVLA+GPG  
Sbjct: 121 RWEENGQPKIVVTCKNQQEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPK 180

[Close]

BLAST of HG10013385 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 129.0 bits (323), Expect = 3.4e-30
Identity = 74/132 (56.06%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60
           M  SS +S + + ++ EK+ +SVSFR EN IPG+VIGFI+G+ LDLS+      K+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPASNNG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRNK-LKD 120
            SK Q+ S    NG DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQ ++ L  
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 AAENIGLPTFVV 131
             E  G P  VV
Sbjct: 121 RWEENGQPKIVV 132

[Close]

BLAST of HG10013385 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.0e-14
Identity = 50/128 (39.06%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 50  SKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    N     K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLADFRKN----FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQR-----NK---------------------LKDAAENIGLPTFVVADAGRTQVSAGSK 151
           L+QR     N+                     L++ A+ + LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

[Close]

BLAST of HG10013385 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.0e-14
Identity = 50/128 (39.06%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 50  SKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    N     K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLADFRKN----FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQR-----NK---------------------LKDAAENIGLPTFVVADAGRTQVSAGSK 151
           L+QR     N+                     L++ A+ + LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

[Close]

BLAST of HG10013385 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-13
Identity = 42/101 (41.58%), Postives = 60/101 (59.41%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN---------------------- 136
           + K+VLVVR DLKMG GKIA+QC+HA  GLY +L++R                       
Sbjct: 55  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDE 114

Query: 137 ----KLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAI-GP 151
               +L++ A+++ LPT +  DAGRTQ++  S+TV+AI GP
Sbjct: 115 DEMLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGP 155

The following BLAST results are available for this feature:

BLAST of HG10013385 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038619.1	7.8e-77	82.72	peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa] >TYK31218.1 peptidyl-tRNA h...	[more]
XP_008466065.1	7.1e-70	82.49	PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo]	[more]
XP_004136220.1	1.6e-69	81.92	peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321....	[more]
KAG7031726.1	4.6e-69	79.12	Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022941498.1	7.9e-69	80.79	peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita moschata] >XP_022981057.1 pe...	[more]

BLAST of HG10013385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y3E5	8.9e-15	37.09	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S...	[more]
Q3ZBL5	5.8e-14	40.95	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=...	[more]
O13830	8.3e-13	31.35	Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]
Q8R2Y8	1.1e-12	39.05	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ...	[more]
O97067	2.4e-12	40.18	Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ...	[more]

BLAST of HG10013385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E5Y4	3.8e-77	82.72	Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3CRS9	3.4e-70	82.49	Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1	[more]
A0A0A0LJW4	7.7e-70	81.92	Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1	[more]
A0A6J1ISX6	3.8e-69	80.79	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480317 PE=4 SV=1	[more]
A0A6J1FS99	3.8e-69	80.79	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446781 PE=4 SV=1	[more]

BLAST of HG10013385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32900.2	1.6e-43	53.85	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.1	3.4e-30	56.06	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.1	2.0e-14	39.06	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.2	2.0e-14	39.06	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT5G16870.1	1.0e-13	41.58	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 114..158 e-value: 1.9E-11 score: 45.8
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 73..113 e-value: 2.3E-14 score: 55.3
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 77..154
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 115..154 e-value: 2.3E-9 score: 37.6 coord: 78..114 e-value: 4.7E-12 score: 46.3
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 114..154 coord: 5..115
None	No IPR available	PANTHER	PTHR12649:SF11	PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL	coord: 114..154 coord: 5..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10013385.1	HG10013385.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity