HG10013374 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10013374
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPeptidase_S9 domain-containing protein
LocationChr02: 856133 .. 860127 (-)
RNA-Seq ExpressionHG10013374
SyntenyHG10013374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAGTTATCGTTGATGCCGACAAGCTTCGGGCCGAATTCCTTCGAGTTCTGCGTAGTAGACGATCTGGAGAAGGTGCGAAATTGAATTTGAATGTCATTACTCTATTCTCTGTTTTAGTTACCTTGTAAATTTTGATGTCTGTTCTGGGTAGCTGGGAAGTTTGTTAATTTGTTTCTTAAATTCTTGCTTGATTGGAATGGTCGCCGTCTATTTGCTGACATGTTTTTTGCTTTTGACATCTTTTGAAAAATGTTTTGCCTTTTCCTGGAAATTAGCAAGTTTTCTTTTGAGGACCATTTTTCTGTCTGTCTATTTGTTTGTGTTTTTAGTCAGCGGGCGTATTATCTTTTCTTGTTAGGATACTTGGTAGCCAATACTAACCTGAAGAAAATTCATGTAAGAAAGTTGAGCTTGGACCCTCATTTTCTTACTTGAGGTAGTGCTTGTTTTGACCAATTAATTAAGTGTAGCAATACGTTCCGGAGATTGTTGTAAAAGTTAAATTTGAAGTTGAATACTATAATTTTCTGTTTCCCGTACCAGTCAGTTGATTGCTATATTGGTTAGGCTACCTTCCGTTTACTTTTCCTTCAACTGAAATTATGAATCTATGATGTCTAATTGAATTTCTCAGTTCCGCTGAATGTGAAGCACACAGAGCCTGTTCTCAACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTTAGTAAGGTTGAATCGTATTATTTCTTCGTTCTTGTATCTTTGTTTAAGTAGTAGTCTACCCATGTTGATTTGTTTTACCTGTTTAAAGGCGATGGCTTCTTGTCCAAAGGCAACTTCTAGCAATTTGAAGGATTTGCTTCACGAGGAAAATCTTCATCTGACTACGGAGGCAAGCTATACTCTCATACTTTTCACATTAACCTATGTGCAGGACATGATTTAACGGTGTATTTAGGAGAAGCTGAATTAGACCCAGAACTTTTCCTCTATGCAAAACATACATTCAGTTTAAGATCTGCTGGCATATTTTTGGAAAAAGAACAATTTCTTGATTTTTGGTCCCTATAAAATTTGAAAGGGAACAAATAGCATGTTCTTTCTTTTTTTTTTTTTTTTGGACAAAACAAATAATGTAGTTTTTCACCAATCATTTATAGGAAGGAGAGCAAGGGCAGTTGCCCATACTGATTATAAGCATGAATGAGAGCAGACAGCAAAAAAGACCGGCAATTGTTTTTCTGCACAGTACAAACAAGTGTAAAGAGTGGTTGAGACCATTGCTTGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCTCCTGGCATTGTTGTTACCAAGTTGAGAGCTAGATCTTACAATATATTCTTGCTTCTTTCAGGCTTATGCATCGAGGGGATATGTAGCTATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGAACAAAACCACCTACCGTGATGTATGCTTATTATTGTTTAAGTTGCTCCCATTTAGCCAATTGAGCTGTCTTTTATCTGTGACCCAAAACTCAAAAATATGTATGCCACTTGACTGGCTTATCGGTTATCACCAATTAGTGAAACTGTGAACACAAGTTCATAAATTTGCTAGAATCTCAGGTTAATCTGGCTGCCAAGGAAGTGATAGACCCTAAATCTAGTTTATTTAAAAGATGTTCGTTTTCTCTTGATTGAGAAAAGTATACCTCTGCTTTTGAGATTGTCAAACCCATGATATGTTAATTGTTAATATGGCTTTTTAGCTCTTTTCTAGTCTCTATAGCGAAGTTTCCTTTTCTTTTGTAGAGTTAAATCCTAACACAATGTCATAATCTGGTGCAGGCTCTTATTTCTTCATGGAAAAGAGGTGATACCATGCCGTTCATATTTGACACGGTAGCTCCCCTTTTCATTTGGTAGCTGAATTGTTTGGCTTATAAATGAACAATTTGCTGCAAATGTAACAACAGGATTAGGAATAACTTCAGTTATACTAATTGAGTGACTTTGGTTTACAGAAGAGCTAATACACTATATCACTTGCAGGTATGGGACTTGATAAAATTGGCAGATTATCTGACGAAAAGGGAGGACATTGACCCATGTAGAATAGGAATTACTGGTGAATCACTTGGAGGTCAGTCAAAAGTAAAATAGAAAGGAACAAATTGGAACATGCCTTTTCTTTGTTATATATATAAGGCTTACCTCTTCACATTTTCATTGCAGGAATGCATGCATGGTTTGGTGCTGCTGCTGACACTCGCTATTCTGTGGTTGCCCCCATAATTGGTGTGCAGGTGGTTAACCCAAATGATGACATTCTGGATCTTGTTAGTATATTCCTTTTTGGAAAGAAGAAAGAGGAGCCAGCTGATATTTTGTGATATGATTGCAGTGTTTTCGATGGGCCATAAATAATGATAAGTGGCAGGCACGAGTTGAGAGTATAAAACCTGTTTTTGAGGGTAAGGTGAATATGGTAATCGGTTAAGGACAGAACACGGTGACTGGTACTGGTTACACACGAAACAGAAGCTAGAAAGAAGAAAACATTAAATAATTTAAAGACAAAACGGATTAATTAATTATTGTTGACTGATAAGCAAGATCATTTTTAAGTTATGATAATTTGAAACAATGAACAACTTTACTAATACACATGACCAGGCTTTGTATCTTGTCTGCAGAAGCACAAATCGAATTAGGCATGAATGGAATCAACAAAGAAGTGGTGAAGAAGGTTTGTTGTTTCAAGTTCTTGGTTTAGTAACTTTTGATCTAGTATTCGGTGTTCTGAATGCAATTTATGAATTATGATCAATCCCAAATCTATCTCTATATTGTGCTTATGATCAATCCCAAACCTATTTCTATATTGTGCTTATTACCAACATGTTGTTTCTTTTCTTCCTTTCTTTTTCTGGACGCTAGAACTTATAGTTACTTTATCTGCTAATAACCAGCTTTTTGATTATATAACACACATTTGAAGTTGAATTACCATAATTATTTCTTACTGTTTTATTCAGGTCTGGAATAGGATTGCTCCTGGTTTAGATTCCCAATTTGACTCGATTTATTCAGTTCCAGCTATTGCACCACGTCCCTTGTTGTTATTAAATGGTAACGTTCATATACAGCCTATACTTGCATGATTTTAATCACAAACCAACCTCAAATTTAGAGGAATTGGAAGATTGAATATGCAGCTCAAACATAACACTTATGCTTTATTTTTAAGGATACATTAGTCCTGACATCATGTCAGTAAACAGGAGGCTATCTCCTTAAAGATGCTGAAATTCACCTGTAAAGTAGGTGGATCTCTTAACTGTCTAGAGATTAATGCCCCAAAACCTGAAAGTTTAAGATTGCGCATATACATATACCATTCCAGGTCTTAAATTAAAATTGAATCGCATCTTTGTGGTCTGTACAAATATAGTTAATGTGCAGTTTGGATTTGAAAAACAATAGGTGCAGAGGATCCTCGATGTCCAATTGCCGGTTTGGATGCTGCCGTATCAAGAACCCAGACAGCTTATCAGAAGTCTGGTTGTCCAGAAAATTTCAAGGTACATATAACTTTCTACCTTCTTTTATTTGTTTAATTAATTTTGGAACCCTTTAAACGAATGGTTATTGTGCATTAAAAATTGTCACTTACAAGTAGCTTACAAGGAAGAACAATTTTATTTATCTAGGAAAATTGGTTATTGGTGATAACAATTTTAAAAAAAATAAGTATACAGAACTTTTAGAAAATAGTTGTTTGATCATATTCTTATCAAATTTCCTTTAAGAAAATAAGTATTATTTTCCCCAAGGGAACTTCCTAACAGAGTGGACTTTTAATTTTAACTGACTTATAATATTTATGTTGTGCAGTTCGTCGCACAACCTGGAATTGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCAAGTGATTGGTTTGACAGGTTTTTATGGCAGAGTATCAAAGAATAA

mRNA sequence

ATGGAAGAAGTTATCGTTGATGCCGACAAGCTTCGGGCCGAATTCCTTCGAGTTCTGCGTAGTAGACGATCTGGAGAAGTTCCGCTGAATGTGAAGCACACAGAGCCTGTTCTCAACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTTAGTAAGGAAGGAGAGCAAGGGCAGTTGCCCATACTGATTATAAGCATGAATGAGAGCAGACAGCAAAAAAGACCGGCAATTGTTTTTCTGCACAGTACAAACAAGTGTAAAGAGTGGTTGAGACCATTGCTTGAGGCTTATGCATCGAGGGGATATGTAGCTATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGAACAAAACCACCTACCGTGATGTATGGGACTTGATAAAATTGGCAGATTATCTGACGAAAAGGGAGGACATTGACCCATGTAGAATAGGAATTACTGGTGAATCACTTGGAGGAATGCATGCATGGTTTGGTGCTGCTGCTGACACTCGCTATTCTGTGGTTGCCCCCATAATTGGTGTGCAGGTGGTTAACCCAAATGATGACATTCTGGATCTTTGTTTTCGATGGGCCATAAATAATGATAAGTGGCAGGCACGAGTTGAGAGTATAAAACCTGTTTTTGAGGAAGCACAAATCGAATTAGGCATGAATGGAATCAACAAAGAAGTGGTGAAGAAGGTCTGGAATAGGATTGCTCCTGGTTTAGATTCCCAATTTGACTCGATTTATTCAGTTCCAGCTATTGCACCACGTCCCTTGTTGTTATTAAATGGTGCAGAGGATCCTCGATGTCCAATTGCCGGTTTGGATGCTGCCGTATCAAGAACCCAGACAGCTTATCAGAAGTCTGGTTGTCCAGAAAATTTCAAGTTCGTCGCACAACCTGGAATTGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCAAGTGATTGGTTTGACAGGTTTTTATGGCAGAGTATCAAAGAATAA

Coding sequence (CDS)

ATGGAAGAAGTTATCGTTGATGCCGACAAGCTTCGGGCCGAATTCCTTCGAGTTCTGCGTAGTAGACGATCTGGAGAAGTTCCGCTGAATGTGAAGCACACAGAGCCTGTTCTCAACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTTAGTAAGGAAGGAGAGCAAGGGCAGTTGCCCATACTGATTATAAGCATGAATGAGAGCAGACAGCAAAAAAGACCGGCAATTGTTTTTCTGCACAGTACAAACAAGTGTAAAGAGTGGTTGAGACCATTGCTTGAGGCTTATGCATCGAGGGGATATGTAGCTATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGAACAAAACCACCTACCGTGATGTATGGGACTTGATAAAATTGGCAGATTATCTGACGAAAAGGGAGGACATTGACCCATGTAGAATAGGAATTACTGGTGAATCACTTGGAGGAATGCATGCATGGTTTGGTGCTGCTGCTGACACTCGCTATTCTGTGGTTGCCCCCATAATTGGTGTGCAGGTGGTTAACCCAAATGATGACATTCTGGATCTTTGTTTTCGATGGGCCATAAATAATGATAAGTGGCAGGCACGAGTTGAGAGTATAAAACCTGTTTTTGAGGAAGCACAAATCGAATTAGGCATGAATGGAATCAACAAAGAAGTGGTGAAGAAGGTCTGGAATAGGATTGCTCCTGGTTTAGATTCCCAATTTGACTCGATTTATTCAGTTCCAGCTATTGCACCACGTCCCTTGTTGTTATTAAATGGTGCAGAGGATCCTCGATGTCCAATTGCCGGTTTGGATGCTGCCGTATCAAGAACCCAGACAGCTTATCAGAAGTCTGGTTGTCCAGAAAATTTCAAGTTCGTCGCACAACCTGGAATTGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCAAGTGATTGGTTTGACAGGTTTTTATGGCAGAGTATCAAAGAATAA

Protein sequence

MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKEGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAIDSRYHGERAKNKTTYRDVWDLIKLADYLTKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASDWFDRFLWQSIKE
Homology
BLAST of HG10013374 vs. NCBI nr
Match: XP_038898085.1 (putative esterase YitV [Benincasa hispida])

HSP 1 Score: 574.3 bits (1479), Expect = 6.6e-160
Identity = 292/372 (78.49%), Postives = 302/372 (81.18%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFS--------- 60
           MEE IVDADK RAEFL VLRSRRSGEVPLNVKH +PVLNPLIQEANPPTFS         
Sbjct: 52  MEEAIVDADKFRAEFLSVLRSRRSGEVPLNVKHAKPVLNPLIQEANPPTFSEAMASCPKA 111

Query: 61  ------------------KEGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                             +EGEQGQLP+LIISM +SRQQKRPAIVFLHSTNKCKEWLRPL
Sbjct: 112 TFSNLKDLLHEENLHLTTEEGEQGQLPVLIISMKDSRQQKRPAIVFLHSTNKCKEWLRPL 171

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAKNKTTYRD                  VWDLIKLADYL
Sbjct: 172 LEAYASRGYVAIAIDSRYHGERAKNKTTYRDALISSWKRGDTMPFIFDTVWDLIKLADYL 231

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           TKREDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ           CFRWA+
Sbjct: 232 TKREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------CFRWAV 291

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 292 DNDKWQARVESIKPVFEEARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 351

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGAEDPRCPIAGLDA VSRTQTAYQK GCPENFKF+ QP IGH+MT EMVKEASD
Sbjct: 352 PLLLLNGAEDPRCPIAGLDAPVSRTQTAYQKFGCPENFKFIPQPEIGHQMTSEMVKEASD 411

BLAST of HG10013374 vs. NCBI nr
Match: KAE8651416.1 (hypothetical protein Csa_000971 [Cucumis sativus])

HSP 1 Score: 565.5 bits (1456), Expect = 3.1e-157
Identity = 288/372 (77.42%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK R EFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK        
Sbjct: 67  MEEAIVDADKFRVEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKVMASCPKE 126

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 127 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 186

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 187 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 246

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 247 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 306

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 307 DNDKWQARVESIKPVFEEARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 366

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+ Q GIGHEMT EMVKEASD
Sbjct: 367 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFITQTGIGHEMTTEMVKEASD 426

BLAST of HG10013374 vs. NCBI nr
Match: XP_011652622.1 (uncharacterized protein LOC101220970 isoform X1 [Cucumis sativus])

HSP 1 Score: 565.5 bits (1456), Expect = 3.1e-157
Identity = 288/372 (77.42%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK R EFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK        
Sbjct: 54  MEEAIVDADKFRVEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKVMASCPKE 113

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 114 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 173

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 174 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 233

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 234 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 293

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 294 DNDKWQARVESIKPVFEEARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 353

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+ Q GIGHEMT EMVKEASD
Sbjct: 354 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFITQTGIGHEMTTEMVKEASD 413

BLAST of HG10013374 vs. NCBI nr
Match: XP_008466042.1 (PREDICTED: putative esterase YitV [Cucumis melo] >KAA0038611.1 putative esterase YitV [Cucumis melo var. makuwa] >TYK31210.1 putative esterase YitV [Cucumis melo var. makuwa])

HSP 1 Score: 563.1 bits (1450), Expect = 1.5e-156
Identity = 286/372 (76.88%), Postives = 300/372 (80.65%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK RAEFLRVLR+RRSGEVPLNVKHTEPVLNPLIQEA+PPTFSK        
Sbjct: 52  MEEAIVDADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKE 111

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 112 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 171

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 172 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 231

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 232 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 291

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARV+SIKPVFEEA+I+LGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 292 DNDKWQARVDSIKPVFEEARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 351

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+AQ GIGHEMT EMVKEASD
Sbjct: 352 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASD 411

BLAST of HG10013374 vs. NCBI nr
Match: KAG7031720.1 (yitV [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556.2 bits (1432), Expect = 1.9e-154
Identity = 281/370 (75.95%), Postives = 296/370 (80.00%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE +VDADK RAEFLRVLR RRSGEVPLNVK ++P+ +PLIQEANPP  SK        
Sbjct: 1   MEEALVDADKFRAEFLRVLRHRRSGEVPLNVKLSKPIQDPLIQEANPPALSKAMESCPKE 60

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILIISM +S+QQK+PAIVFLHSTNKCKEWLRPL
Sbjct: 61  DISNLKDLLHVENLHLATEEGEQGQLPILIISMKDSKQQKKPAIVFLHSTNKCKEWLRPL 120

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAKNKTTYRD                  VWDLIKLADYL
Sbjct: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRDALVSSWKRGDTMPFIFDTVWDLIKLADYL 180

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           TKREDIDPCRIGITGESLGGMHAWF AAADTRY+VV PIIGVQ           CFRWAI
Sbjct: 181 TKREDIDPCRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQ-----------CFRWAI 240

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVV KVWNRIAPGLDS+FDSIYSVPA+APR
Sbjct: 241 DNDKWQARVESIKPVFEEARIELGMNEINKEVVTKVWNRIAPGLDSKFDSIYSVPAVAPR 300

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 326
           PLLLLNGA+DPRCPIAGLDA VSRTQT YQ  GCPENFKF+AQPGIGH MTPEMVKEASD
Sbjct: 301 PLLLLNGADDPRCPIAGLDATVSRTQTVYQNFGCPENFKFIAQPGIGHAMTPEMVKEASD 359

BLAST of HG10013374 vs. ExPASy Swiss-Prot
Match: O34973 (Putative hydrolase YtaP OS=Bacillus subtilis (strain 168) OX=224308 GN=ytaP PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 6.5e-09
Identity = 48/197 (24.37%), Postives = 75/197 (38.07%), Query Frame = 0

Query: 125 VWDLIKLADYLTKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPND 184
           ++D +   DY+  R D+ P RIG  G S+GG+ AW+ AA D R  V              
Sbjct: 142 IYDSLSALDYMQSRSDVQPDRIGTIGMSMGGLMAWWTAALDDRIKV-------------- 201

Query: 185 DILDLCFRWAINNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFD 244
             +DLC                       +Q++  +    + + +  +    P L   F 
Sbjct: 202 -CVDLC-----------------------SQVDHHVLIKTQNLDRHGFYYYVPSLAKHFS 261

Query: 245 SIYSVPAIAPRPLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHE 304
           +      IAPRP L L G  D   P  G+D         Y   G  + ++ V +   GH 
Sbjct: 262 ASEIQSLIAPRPHLSLVGVHDRLTPAEGVDKIEKELTAVYAGQGAADCYR-VVRSASGHF 299

Query: 305 MTPEMVKEASDWFDRFL 322
            T  +  EA  +  ++L
Sbjct: 322 ETAVIRHEAVRFLQKWL 299

BLAST of HG10013374 vs. ExPASy Swiss-Prot
Match: P39839 (Uncharacterized peptidase YuxL OS=Bacillus subtilis (strain 168) OX=224308 GN=yuxL PE=3 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-05
Identity = 61/244 (25.00%), Postives = 94/244 (38.52%), Query Frame = 0

Query: 95  EAYASRGYVAIAIDSR-YHG------ERAKNKTTYRDVWDLIKLADYLTKRE-DIDPCRI 154
           +  A++GY  + I+ R  HG         +     +D  D+++  D   KR+  IDP R+
Sbjct: 453 QVLAAKGYAVVYINPRGSHGYGQEFVNAVRGDYGGKDYDDVMQAVDEAIKRDPHIDPKRL 512

Query: 155 GITGESLGG-MHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINNDKWQARVE 214
           G+TG S GG M  W                   +V   +       + +I+N      V 
Sbjct: 513 GVTGGSYGGFMTNW-------------------IVGQTNRFKAAVTQRSISNWISFHGVS 572

Query: 215 SIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGAED 274
            I   F + Q+E  M     E  +K+W+R          S     A    PLL+L+G  D
Sbjct: 573 DIGYFFTDWQLEHDM----FEDTEKLWDR----------SPLKYAANVETPLLILHGERD 632

Query: 275 PRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTP--------EMVKEASDWF 322
            RCPI        +   A +K G  +  K V  P   H ++         + +   S WF
Sbjct: 633 DRCPI----EQAEQLFIALKKMG--KETKLVRFPNASHNLSRTGHPRQRIKRLNYISSWF 657

BLAST of HG10013374 vs. ExPASy Swiss-Prot
Match: Q99390 (Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia coli OX=562 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.3e-05
Identity = 32/103 (31.07%), Postives = 45/103 (43.69%), Query Frame = 0

Query: 73  KRPAIVFLHSTNKCKEWLRP-LLEAYASRGYVAIAIDSRYHGERAKNK---TTYRDVWDL 132
           K P I+  H     +  L P    A+   G+  I  D R  GE    +          D+
Sbjct: 24  KHPLIILCHGFCGIRNVLLPCFANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDI 83

Query: 133 IKLADYLTKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVV 172
           I + ++  K+E ID  RIG+ G SLGG H +  AA D R   +
Sbjct: 84  ISVINWAEKQECIDNQRIGLWGTSLGGGHVFSAAAQDQRVKCI 126

BLAST of HG10013374 vs. ExPASy Swiss-Prot
Match: P29368 (Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia coli OX=562 PE=4 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.3e-04
Identity = 31/103 (30.10%), Postives = 44/103 (42.72%), Query Frame = 0

Query: 73  KRPAIVFLHSTNKCKEWLRP-LLEAYASRGYVAIAIDSRYHGERAKNK---TTYRDVWDL 132
           K P I+  H     +  L P    A+   G+  I  D R  GE    +          D+
Sbjct: 24  KHPLIILCHGFCGIRNVLLPCFANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDI 83

Query: 133 IKLADYLTKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVV 172
           I + ++  K+E ID  RIG+ G SLGG H +   A D R   +
Sbjct: 84  ISVINWAEKQECIDNQRIGLWGTSLGGGHVFSARAQDQRVKCI 126

BLAST of HG10013374 vs. ExPASy TrEMBL
Match: A0A0A0LJX7 (Peptidase_S9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G895770 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.5e-157
Identity = 288/372 (77.42%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK R EFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK        
Sbjct: 54  MEEAIVDADKFRVEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKVMASCPKE 113

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 114 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 173

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 174 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 233

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 234 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 293

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 294 DNDKWQARVESIKPVFEEARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 353

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+ Q GIGHEMT EMVKEASD
Sbjct: 354 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFITQTGIGHEMTTEMVKEASD 413

BLAST of HG10013374 vs. ExPASy TrEMBL
Match: A0A5A7TAI4 (Putative esterase YitV OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004950 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.4e-157
Identity = 286/372 (76.88%), Postives = 300/372 (80.65%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK RAEFLRVLR+RRSGEVPLNVKHTEPVLNPLIQEA+PPTFSK        
Sbjct: 52  MEEAIVDADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKE 111

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 112 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 171

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 172 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 231

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 232 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 291

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARV+SIKPVFEEA+I+LGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 292 DNDKWQARVDSIKPVFEEARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 351

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+AQ GIGHEMT EMVKEASD
Sbjct: 352 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASD 411

BLAST of HG10013374 vs. ExPASy TrEMBL
Match: A0A1S3CRR2 (putative esterase YitV OS=Cucumis melo OX=3656 GN=LOC103503593 PE=4 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.4e-157
Identity = 286/372 (76.88%), Postives = 300/372 (80.65%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE IVDADK RAEFLRVLR+RRSGEVPLNVKHTEPVLNPLIQEA+PPTFSK        
Sbjct: 52  MEEAIVDADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKE 111

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILI+SM ESRQQKRP IVFLHSTNKCKEWLRPL
Sbjct: 112 TSCNLKDLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPL 171

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAK KTTYRD                  VWDLIKLADYL
Sbjct: 172 LEAYASRGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYL 231

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           T+REDIDPCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ            F WA+
Sbjct: 232 TRREDIDPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------SFGWAV 291

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARV+SIKPVFEEA+I+LGMN INKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR
Sbjct: 292 DNDKWQARVDSIKPVFEEARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 351

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 328
           PLLLLNGA+DPRCP+AGLDA VSR QTAYQK GCPENFKF+AQ GIGHEMT EMVKEASD
Sbjct: 352 PLLLLNGADDPRCPVAGLDAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASD 411

BLAST of HG10013374 vs. ExPASy TrEMBL
Match: A0A6J1FKQ9 (uncharacterized protein LOC111446637 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446637 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.4e-153
Identity = 277/370 (74.86%), Postives = 294/370 (79.46%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           MEE +VDADK  AEFLRVLR RRSGEVPLNVK ++P+ +PLIQEANPP  SK        
Sbjct: 1   MEEALVDADKFGAEFLRVLRHRRSGEVPLNVKLSKPIQDPLIQEANPPALSKAMESCPKE 60

Query: 61  -------------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPL 120
                              EGEQGQLPILIISM +S+QQK+PAIVFLHSTNKCKEWLRPL
Sbjct: 61  DISNLKDLLHEENLHLATEEGEQGQLPILIISMKDSKQQKKPAIVFLHSTNKCKEWLRPL 120

Query: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYL 180
           LEAYASRGYVAIAIDSRYHGERAKNKTTYRD                  VWDLIKLADYL
Sbjct: 121 LEAYASRGYVAIAIDSRYHGERAKNKTTYRDALVSSWKRGDTMPFIFDTVWDLIKLADYL 180

Query: 181 TKREDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAI 240
           TKREDIDPCRIGITGESLGGMHAWF AAADTRY+VV PIIGVQ           CFRWAI
Sbjct: 181 TKREDIDPCRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQ-----------CFRWAI 240

Query: 241 NNDKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPR 300
           +NDKWQARVESIKPVFEEA+IELGMN INKEVV KVWNRIAPGLDS+FDSIY+VPA+APR
Sbjct: 241 DNDKWQARVESIKPVFEEARIELGMNEINKEVVTKVWNRIAPGLDSKFDSIYAVPAVAPR 300

Query: 301 PLLLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASD 326
           PLLLLNGA+DPRCPIAGLD  V+RTQT YQ  GCPENFKF+AQPGIGH MTPEMVKEASD
Sbjct: 301 PLLLLNGADDPRCPIAGLDTTVTRTQTVYQNFGCPENFKFIAQPGIGHAMTPEMVKEASD 359

BLAST of HG10013374 vs. ExPASy TrEMBL
Match: A0A6J1IF89 (uncharacterized protein LOC111476405 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476405 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.1e-152
Identity = 281/365 (76.99%), Postives = 293/365 (80.27%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSK-------- 60
           M+E +VDADK RAEFLRVLRSRRS EVPLNVK T PV +PLIQE NP TFSK        
Sbjct: 42  MDEAVVDADKFRAEFLRVLRSRRSPEVPLNVKRTPPVHHPLIQEPNPTTFSKATFSNLKD 101

Query: 61  ------------EGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPLLEAYASR 120
                       EGEQG+LPILIISM +SRQQKRPAIVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 102 LLHEENLHLTTEEGEQGKLPILIISMKDSRQQKRPAIVFLHSTNKCKEWLRPLLEAYASR 161

Query: 121 GYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYLTKREDID 180
           GYVAIAIDSRYHGERAKNK+TY D                  VWDLIKLADYLTKREDID
Sbjct: 162 GYVAIAIDSRYHGERAKNKSTYYDALISSWKKGDTMPFIFDTVWDLIKLADYLTKREDID 221

Query: 181 PCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINNDKWQA 240
           PCRIGITGESLGGMHAWF AAADTRYSVV PIIGVQ           CFRWAI+NDKWQA
Sbjct: 222 PCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQ-----------CFRWAIDNDKWQA 281

Query: 241 RVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNG 300
           RVESIKPVFEEA+IELGMN IN EVVKKVWNRIAPGL SQF SIYSVPAIAPRPLLLLNG
Sbjct: 282 RVESIKPVFEEARIELGMNEINIEVVKKVWNRIAPGLVSQFGSIYSVPAIAPRPLLLLNG 341

Query: 301 AEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASDWFDRFLW 328
           A+DPRCPIAGLDA VS TQ AYQ+ GCPENFKF+AQPGIGHEMTPEMVKEAS WFDRFL 
Sbjct: 342 ADDPRCPIAGLDALVSETQVAYQRFGCPENFKFIAQPGIGHEMTPEMVKEASQWFDRFLG 395

BLAST of HG10013374 vs. TAIR 10
Match: AT5G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 205/366 (56.01%), Postives = 249/366 (68.03%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPT----------- 60
           ME  I      R +FLR+L SRRS +VPL    + P+ NPL Q   P T           
Sbjct: 1   MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 60

Query: 61  --------------FSKEGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPLLE 120
                          +++ EQG+LP+LI+S+ E  ++KRPAIVF+H TN  KEWLRP LE
Sbjct: 61  GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 120

Query: 121 AYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYLTK 180
           AYASRGYVAI +DSRYHGERA  KT YRD                  VWDLIKLA+YLT+
Sbjct: 121 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 180

Query: 181 REDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINN 240
           R+DIDP +IGITG SLGGMHAWF AAADTRYSVV P+IGVQ            FRWAI N
Sbjct: 181 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQ-----------GFRWAIEN 240

Query: 241 DKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPL 300
           D+W+ARV SIKP+FEEA+I+LG N I+KE+V+KVWNRIAPGL S+FDS YS+P IAPRPL
Sbjct: 241 DEWEARVNSIKPLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPL 300

Query: 301 LLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASDWF 324
            +LNGA DPRCP+ GL+ A+ R + AY+++  P NFKF A+ G+GHE T  M+KE+SDWF
Sbjct: 301 YILNGANDPRCPLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWF 355

BLAST of HG10013374 vs. TAIR 10
Match: AT5G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 205/366 (56.01%), Postives = 249/366 (68.03%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPT----------- 60
           ME  I      R +FLR+L SRRS +VPL    + P+ NPL Q   P T           
Sbjct: 59  MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 118

Query: 61  --------------FSKEGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPLLE 120
                          +++ EQG+LP+LI+S+ E  ++KRPAIVF+H TN  KEWLRP LE
Sbjct: 119 GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 178

Query: 121 AYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYLTK 180
           AYASRGYVAI +DSRYHGERA  KT YRD                  VWDLIKLA+YLT+
Sbjct: 179 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 238

Query: 181 REDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINN 240
           R+DIDP +IGITG SLGGMHAWF AAADTRYSVV P+IGVQ            FRWAI N
Sbjct: 239 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQ-----------GFRWAIEN 298

Query: 241 DKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPL 300
           D+W+ARV SIKP+FEEA+I+LG N I+KE+V+KVWNRIAPGL S+FDS YS+P IAPRPL
Sbjct: 299 DEWEARVNSIKPLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPL 358

Query: 301 LLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASDWF 324
            +LNGA DPRCP+ GL+ A+ R + AY+++  P NFKF A+ G+GHE T  M+KE+SDWF
Sbjct: 359 YILNGANDPRCPLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWF 413

BLAST of HG10013374 vs. TAIR 10
Match: AT5G25770.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 205/366 (56.01%), Postives = 249/366 (68.03%), Query Frame = 0

Query: 1   MEEVIVDADKLRAEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPT----------- 60
           ME  I      R +FLR+L SRRS +VPL    + P+ NPL Q   P T           
Sbjct: 62  MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 121

Query: 61  --------------FSKEGEQGQLPILIISMNESRQQKRPAIVFLHSTNKCKEWLRPLLE 120
                          +++ EQG+LP+LI+S+ E  ++KRPAIVF+H TN  KEWLRP LE
Sbjct: 122 GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 181

Query: 121 AYASRGYVAIAIDSRYHGERAKNKTTYRD------------------VWDLIKLADYLTK 180
           AYASRGYVAI +DSRYHGERA  KT YRD                  VWDLIKLA+YLT+
Sbjct: 182 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 241

Query: 181 REDIDPCRIGITGESLGGMHAWFGAAADTRYSVVAPIIGVQVVNPNDDILDLCFRWAINN 240
           R+DIDP +IGITG SLGGMHAWF AAADTRYSVV P+IGVQ            FRWAI N
Sbjct: 242 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQ-----------GFRWAIEN 301

Query: 241 DKWQARVESIKPVFEEAQIELGMNGINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPL 300
           D+W+ARV SIKP+FEEA+I+LG N I+KE+V+KVWNRIAPGL S+FDS YS+P IAPRPL
Sbjct: 302 DEWEARVNSIKPLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPL 361

Query: 301 LLLNGAEDPRCPIAGLDAAVSRTQTAYQKSGCPENFKFVAQPGIGHEMTPEMVKEASDWF 324
            +LNGA DPRCP+ GL+ A+ R + AY+++  P NFKF A+ G+GHE T  M+KE+SDWF
Sbjct: 362 YILNGANDPRCPLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWF 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898085.16.6e-16078.49putative esterase YitV [Benincasa hispida][more]
KAE8651416.13.1e-15777.42hypothetical protein Csa_000971 [Cucumis sativus][more]
XP_011652622.13.1e-15777.42uncharacterized protein LOC101220970 isoform X1 [Cucumis sativus][more]
XP_008466042.11.5e-15676.88PREDICTED: putative esterase YitV [Cucumis melo] >KAA0038611.1 putative esterase... [more]
KAG7031720.11.9e-15475.95yitV [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
O349736.5e-0924.37Putative hydrolase YtaP OS=Bacillus subtilis (strain 168) OX=224308 GN=ytaP PE=3... [more]
P398392.5e-0525.00Uncharacterized peptidase YuxL OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
Q993903.3e-0531.07Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia c... [more]
P293681.3e-0430.10Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia c... [more]
Match NameE-valueIdentityDescription
A0A0A0LJX71.5e-15777.42Peptidase_S9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G89577... [more]
A0A5A7TAI47.4e-15776.88Putative esterase YitV OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A1S3CRR27.4e-15776.88putative esterase YitV OS=Cucumis melo OX=3656 GN=LOC103503593 PE=4 SV=1[more]
A0A6J1FKQ98.4e-15374.86uncharacterized protein LOC111446637 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IF891.1e-15276.99uncharacterized protein LOC111476405 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G25770.11.1e-11256.01alpha/beta-Hydrolases superfamily protein [more]
AT5G25770.21.1e-11256.01alpha/beta-Hydrolases superfamily protein [more]
AT5G25770.31.1e-11256.01alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 50..324
e-value: 3.8E-43
score: 149.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 71..321
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 97..306
e-value: 2.7E-8
score: 33.6
NoneNo IPR availablePANTHERPTHR47381:SF2ESTERASE YITV-RELATEDcoord: 4..53
NoneNo IPR availablePANTHERPTHR47381:SF2ESTERASE YITV-RELATEDcoord: 53..321
NoneNo IPR availablePANTHERPTHR47381ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 4..53
NoneNo IPR availablePANTHERPTHR47381ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 53..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10013374.1HG10013374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008236 serine-type peptidase activity