HG10013355 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10013355 vs. NCBI nr
Match: XP_038897933.1 (serine hydroxymethyltransferase 7-like [Benincasa hispida] >XP_038897934.1 serine hydroxymethyltransferase 7-like [Benincasa hispida])

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 545/582 (93.64%), Postives = 560/582 (96.22%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFR QKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRHQKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           V DDDSEDEKFSLLG+P RVKR+RDA+SFLNQ +AKR AVADEASLETRR  VRAWGNQ 
Sbjct: 61  VVDDDSEDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVADEASLETRRALVRAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           IRIADPEIHNIMAKERQRQ KGIELVASENFVCRAVMEALGSHLTNKYSEGMPG+RYYTG
Sbjct: 121 IRIADPEIHNIMAKERQRQCKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGSRYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCCARALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDR+MGLDS S
Sbjct: 181 NQYIDEIELLCCARALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRLMGLDSTS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LTHGDD  T TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDTGTGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEY+VYI+QVKKNAQALASALLRRKC+LVTNGTDNHLVLWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYKVYIRQVKKNAQALASALLRRKCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILL+A +ITKIVIR
Sbjct: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLQAARITKIVIR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHKDIM NLQNN DIVELRNRVEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNNDIVELRNRVEAFAASFAMPGFDI 582

BLAST of HG10013355 vs. NCBI nr
Match: XP_008444112.1 (PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis melo] >KAA0064247.1 serine hydroxymethyltransferase 7-like [Cucumis melo var. makuwa] >TYK18617.1 serine hydroxymethyltransferase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 537/582 (92.27%), Postives = 557/582 (95.70%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+FRQQKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           V DDDS+DEKFSLLG+P RVKR+RDA+SFL+Q +AKR AV DEASLETRR SV+ WGNQ 
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCCARALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LTHGDD   +TYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEYRVYI+QVKKNAQALASALLRR C+LVTNGTDNHL+LWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNK AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA  ITKIVIR
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHKDIM NLQNNKDIVELRNRVE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of HG10013355 vs. NCBI nr
Match: XP_004142612.1 (serine hydroxymethyltransferase 7 [Cucumis sativus] >KGN54611.1 hypothetical protein Csa_012900 [Cucumis sativus])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 533/582 (91.58%), Postives = 555/582 (95.36%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+F Q KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           VADDDS+DEKFSLLG+P RVKR+RDA+SFLNQ +AKR AV DE+SL+ RRV V+AWGNQ 
Sbjct: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LT GDD  T+TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTRGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEYRVYI+QVKKNAQALASALLRR C+LVTNGTDNHLVLWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETI +ILLKA  ITKIV+R
Sbjct: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHK IM NLQNNKDIVELRN+VEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 582

BLAST of HG10013355 vs. NCBI nr
Match: XP_022980970.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.0e-296
Identity = 509/580 (87.76%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  E R+QKDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
             DD S+DEKFSLLG+P RVKR+RD+ SFLNQ +AKR+A+ DE+SLE RR SVRAWGNQ 
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIH+IMAKE+QRQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIA+KCGAILM DM+HISGLVAAKECVSPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGV L HGD+T TYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEA 480
           AS EYRVYI+QVKKNAQALASALLRRKC+LVT+GTDNHLVLWDL PLGLTAK+YE ICE 
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRG 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA QITK+V+ R 
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHR- 540

Query: 541 KFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           + GKLHKDIM NLQNNKDI+ELRNRVEAFAASFAMPGFDI
Sbjct: 541 EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of HG10013355 vs. NCBI nr
Match: KAG7031702.1 (Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.7e-296
Identity = 507/580 (87.41%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  E R+QKDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
             DD S+DEKFSLLG+P RVKR+RD+ SFLNQ +AKR+A+ DE+SLE RR SVRAWGNQ 
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIH+IMAKE+QRQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIA+KCGAILM DM+HISGLVAAKEC SPF+YCD+VTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGV L HG++T TYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEA 480
           AS EYRVYI+QVKKNAQALASALLRRKC+LVT+GTDNHLVLWDL PLGLTAK+YE ICE 
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRG 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA QITK+V+ R 
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHR- 540

Query: 541 KFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           + GKLHKDIM NLQNNKDI+ELRNRVEAFAASFAMPGFDI
Sbjct: 541 EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of HG10013355 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 5.7e-226
Identity = 393/575 (68.35%), Postives = 456/575 (79.30%), Query Frame = 0

Query: 15  SSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFR-------QQKDEKFDLVK------GV 74
           +S T +   R+   D+S  L    S  S P+ F        +QK EK   V       G 
Sbjct: 19  ASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDGGG 78

Query: 75  ADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQPI 134
             D  EDE F +LG    +KR+RD    L Q    + +   ++ LE+RR +VRAWG+QPI
Sbjct: 79  GGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQPI 138

Query: 135 RIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 194
            +ADP+IH +M KE+QRQ +GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN
Sbjct: 139 HLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 198

Query: 195 QYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASG 254
           QYID+IE LC  RAL AF L+ +KWGVNVQPYSCTSANFAVYTGLLLP +RIMGLDS SG
Sbjct: 199 QYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSPSG 258

Query: 255 GHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSY 314
           GH+SHGY +P GKKISA SIFFES PY+VNP TGY+DYDKLE+KALDYRPKILICGGSSY
Sbjct: 259 GHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGSSY 318

Query: 315 PREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRGG 374
           PR+WD+AR RQIA+KCGA+LMCDMAHISGLVA KEC +PF++CDIVTSTTHK LRGPRGG
Sbjct: 319 PRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPRGG 378

Query: 375 IIFFRRGLKSRKQGVLTHGDDTST-YDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVA 434
           IIF+RRG K RKQG  +   DTST YD E++INF+VFPSLQGGPHNNHIAALAIALKQVA
Sbjct: 379 IIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQVA 438

Query: 435 SPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEAC 494
           +PEY+ YIQQ+KKNAQALA+ALLRRKC+LVT GTDNHL+LWDLTP+GLT K YE +CE C
Sbjct: 439 TPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCEMC 498

Query: 495 HITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRGK 554
           HIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFET+ D L+KA QIT  + R  +
Sbjct: 499 HITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITSALQR--E 558

Query: 555 FGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMP 576
            GK HK+ + +L  NKDI ELRNRVEAFA  + MP
Sbjct: 559 HGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of HG10013355 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 3.6e-220
Identity = 372/532 (69.92%), Postives = 439/532 (82.52%), Query Frame = 0

Query: 53  EKFDLVKGVADDDS--EDEKFSLLGKPFRVKRERDANSF--LNQVAAKRVAVADEASLET 112
           E+ D V G  DDD   E+E+F LLG P ++KR R  NS+   +    KR  V  ++ +E+
Sbjct: 72  EEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVV--DSGIES 131

Query: 113 RRVSVRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKY 172
           RR  VRAWGNQ I  ADPEIH  M KE+QRQ++GIEL+ASENFVCRAVMEALGSHLTNKY
Sbjct: 132 RRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKY 191

Query: 173 SEGMPGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLL 232
           SEGMPGARYYTGNQYID+IE+LC  RALAAF L+ EKWGVNVQPYSCTSANFAV+TGLL+
Sbjct: 192 SEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLM 251

Query: 233 PKDRIMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALD 292
           P +RIMGLDS SGGH+SHGYY+P GKK+S  SIFFES PY+V+P TGY+DYDKLEEKALD
Sbjct: 252 PGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALD 311

Query: 293 YRPKILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVT 352
           YRPKILICGGSSYPR+W++ R R IA+KCGA+LM DMA ISGLVAAKE  +PF+YCDIVT
Sbjct: 312 YRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVT 371

Query: 353 STTHKSLRGPRGGIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNN 412
           STTHKSLRGPRGGIIF++RGLK +KQ + L H +    YDFE++INFSVFPSLQGGPHNN
Sbjct: 372 STTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNN 431

Query: 413 HIAALAIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLG 472
           HIAALAIALKQ ASPEY++Y++QVKKNA+ALASAL+ RKCKL+T GTDNHL+LWDLTPLG
Sbjct: 432 HIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLG 491

Query: 473 LTAKDYEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLK 532
           LT K YE +CE CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FET+ D L +
Sbjct: 492 LTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYR 551

Query: 533 ATQITKIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           A QI     R  + GKL K+ + ++ + K+I +LRN+VEAFA  FAMP FD+
Sbjct: 552 AAQIASAAQR--EHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of HG10013355 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.5e-173
Identity = 292/465 (62.80%), Postives = 365/465 (78.49%), Query Frame = 0

Query: 113 VRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  +   DPEIH+++ KE++RQ +GIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDR 232
           PG RYY GN++IDEIE LC +RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYY+  GKKISATSI+FESLPY+VN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTH 352
           +LICGGS+YPR+WDYAR R IA+K GA+L+CDMAHISGLVAA+E  +PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAAL 412
           KSLRGPR G+IF+R+G K  K+G      + + YDFED+INF+VFP+LQGGPHN+ I AL
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGAL 303

Query: 413 AIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKD 472
           A+ALKQ  +P ++VY +QVK NA AL + L+ +  ++VTNGT+NHLVLWDL PLGLT   
Sbjct: 304 AVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNK 363

Query: 473 YEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQIT 532
            E +C+ C IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE I + L +A  +T
Sbjct: 364 VEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLT 423

Query: 533 KIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGF 578
             +  +  +GKL KD    L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 LDI--QKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of HG10013355 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.7e-167
Identity = 282/467 (60.39%), Postives = 355/467 (76.02%), Query Frame = 0

Query: 113 VRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  +   DPEI++++ KE+ RQ +GIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDR 232
           PG RYY G ++IDEIE LC +R+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYYS  GK ISATSI+FE+LPY+V+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTH 352
           ++ICGG+SYPREWDYAR R +A+K GA L+CDMAH S LVAA+E   PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAAL 412
           KSLRGPR G+IF+R+G K  K+G      +   YDF+ +IN +VFP+LQ GPHNN I AL
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGAL 303

Query: 413 AIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKD 472
           A+ALKQV +P ++VY +QVK NA  LAS L+ +   LVT+GTDNHL+LWDL PLGLT   
Sbjct: 304 AVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNK 363

Query: 473 YEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQIT 532
            E +CE C+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE I + L +A  IT
Sbjct: 364 VEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTIT 423

Query: 533 KIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
             +  + ++GK+ KD    L NNK+I E++  VE F   F MPGF I
Sbjct: 424 LDI--QEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of HG10013355 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.3e-153
Identity = 266/460 (57.83%), Postives = 338/460 (73.48%), Query Frame = 0

Query: 117 GNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGAR 176
           GN P++  D EI  +M +E+ RQ+KG+EL+ASENF  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 177 YYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGL 236
           YY G + +DE+E LC  RAL AF LD  KWGVNVQPYS + ANFAVYT LL P DRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 237 DSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILIC 296
           D  SGGHL+HGY +   KKISA+SIFFES+PY++    G +DY +LEE AL ++PK++I 
Sbjct: 126 DLPSGGHLTHGYQTD-KKKISASSIFFESMPYQIG-ADGLIDYQRLEENALLFKPKLIIS 185

Query: 297 GGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLR 356
           G S+YPREWDY R R IA+K GA LMCDMAH SGLVAA+   SPF+YCD+VTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 357 GPRGGIIFFRRGLKSRKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIAL 416
           GPR GIIFFRRG +     V  +G +   YD E +INF+VFPSLQGGPH N IA +A+AL
Sbjct: 246 GPRSGIIFFRRGKR-----VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVAL 305

Query: 417 KQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMI 476
           K+  S E++ Y  QVKKNA A+ +AL+ +  KLVTNGTDNHL+LWDL P  LT   +E  
Sbjct: 306 KEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKA 365

Query: 477 CEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVI 536
            +  +ITVNK A++GD  AISPGG+RIG+ A+T+RG  EADFE I D L +   I+  + 
Sbjct: 366 ADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI- 425

Query: 537 RRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPG 577
            +G+ GK   D ++ +  +K++++LR  VE F++ F +PG
Sbjct: 426 -QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of HG10013355 vs. ExPASy TrEMBL
Match: A0A5A7VB49 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001500 PE=3 SV=1)

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 537/582 (92.27%), Postives = 557/582 (95.70%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+FRQQKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           V DDDS+DEKFSLLG+P RVKR+RDA+SFL+Q +AKR AV DEASLETRR SV+ WGNQ 
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCCARALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LTHGDD   +TYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEYRVYI+QVKKNAQALASALLRR C+LVTNGTDNHL+LWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNK AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA  ITKIVIR
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHKDIM NLQNNKDIVELRNRVE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of HG10013355 vs. ExPASy TrEMBL
Match: A0A1S3B9K5 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=1)

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 537/582 (92.27%), Postives = 557/582 (95.70%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+FRQQKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           V DDDS+DEKFSLLG+P RVKR+RDA+SFL+Q +AKR AV DEASLETRR SV+ WGNQ 
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCCARALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LTHGDD   +TYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEYRVYI+QVKKNAQALASALLRR C+LVTNGTDNHL+LWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNK AIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA  ITKIVIR
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHKDIM NLQNNKDIVELRNRVE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

BLAST of HG10013355 vs. ExPASy TrEMBL
Match: A0A0A0L3A9 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G377740 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 533/582 (91.58%), Postives = 555/582 (95.36%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+F Q KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
           VADDDS+DEKFSLLG+P RVKR+RDA+SFLNQ +AKR AV DE+SL+ RRV V+AWGNQ 
Sbjct: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIA+KCGA+LMCDMAHISGLVAAKEC SPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDD--TSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGV LT GDD  T+TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTRGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMIC 480
           QVASPEYRVYI+QVKKNAQALASALLRR C+LVTNGTDNHLVLWDLTPLGLTAK+YEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIR 540
           EACHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETI +ILLKA  ITKIV+R
Sbjct: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540

Query: 541 RGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           RGK GKLHK IM NLQNNKDIVELRN+VEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 582

BLAST of HG10013355 vs. ExPASy TrEMBL
Match: A0A6J1IY33 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 4.9e-297
Identity = 509/580 (87.76%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  E R+QKDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
             DD S+DEKFSLLG+P RVKR+RD+ SFLNQ +AKR+A+ DE+SLE RR SVRAWGNQ 
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIH+IMAKE+QRQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIA+KCGAILM DM+HISGLVAAKECVSPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGV L HGD+T TYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEA 480
           AS EYRVYI+QVKKNAQALASALLRRKC+LVT+GTDNHLVLWDL PLGLTAK+YE ICE 
Sbjct: 421 ASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRG 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA QITK+V+ R 
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHR- 540

Query: 541 KFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           + GKLHKDIM NLQNNKDI+ELRNRVEAFAASFAMPGFDI
Sbjct: 541 EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of HG10013355 vs. ExPASy TrEMBL
Match: A0A6J1FSB1 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE=3 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.3e-296
Identity = 505/580 (87.07%), Postives = 540/580 (93.10%), Query Frame = 0

Query: 1   MDFRHAHSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRQQKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  E R+QKDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQP 120
             DD S+DE+FSLLG+P RVKR+RD+ SFLNQ +AKR+A+ DE+SLE RR SVRAWGNQ 
Sbjct: 61  DPDDHSDDEQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQS 120

Query: 121 IRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIH+IMAKE+QRQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKI+ICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSS 300

Query: 301 YPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIA+KCGAILM DM+HISGLVAAKECVSPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420
           GIIFFRRGLKSRKQGV L HG++T TYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQV 420

Query: 421 ASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEA 480
            S EYRVYI+QVKKNAQALASALLRRKC+LVT+GTDNHLVLWDL PLGLTAK+YE ICE 
Sbjct: 421 TSLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKICEL 480

Query: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRG 540
           CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETI +ILLKA QITK+V+ R 
Sbjct: 481 CHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLHR- 540

Query: 541 KFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           + GKLHKD+M NLQNNKDI+ELRNRVEAFAASFAMPGFDI
Sbjct: 541 EHGKLHKDMMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

BLAST of HG10013355 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 785.0 bits (2026), Expect = 4.1e-227
Identity = 393/575 (68.35%), Postives = 456/575 (79.30%), Query Frame = 0

Query: 15  SSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFR-------QQKDEKFDLVK------GV 74
           +S T +   R+   D+S  L    S  S P+ F        +QK EK   V       G 
Sbjct: 19  ASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDGGG 78

Query: 75  ADDDSEDEKFSLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQPI 134
             D  EDE F +LG    +KR+RD    L Q    + +   ++ LE+RR +VRAWG+QPI
Sbjct: 79  GGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQPI 138

Query: 135 RIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 194
            +ADP+IH +M KE+QRQ +GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN
Sbjct: 139 HLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 198

Query: 195 QYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASG 254
           QYID+IE LC  RAL AF L+ +KWGVNVQPYSCTSANFAVYTGLLLP +RIMGLDS SG
Sbjct: 199 QYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSPSG 258

Query: 255 GHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSY 314
           GH+SHGY +P GKKISA SIFFES PY+VNP TGY+DYDKLE+KALDYRPKILICGGSSY
Sbjct: 259 GHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGSSY 318

Query: 315 PREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRGG 374
           PR+WD+AR RQIA+KCGA+LMCDMAHISGLVA KEC +PF++CDIVTSTTHK LRGPRGG
Sbjct: 319 PRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPRGG 378

Query: 375 IIFFRRGLKSRKQGVLTHGDDTST-YDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVA 434
           IIF+RRG K RKQG  +   DTST YD E++INF+VFPSLQGGPHNNHIAALAIALKQVA
Sbjct: 379 IIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQVA 438

Query: 435 SPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEAC 494
           +PEY+ YIQQ+KKNAQALA+ALLRRKC+LVT GTDNHL+LWDLTP+GLT K YE +CE C
Sbjct: 439 TPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCEMC 498

Query: 495 HITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRGK 554
           HIT+NK AI+GDNG ISPGGVRIGTPAMTTRGC+E+DFET+ D L+KA QIT  + R  +
Sbjct: 499 HITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITSALQR--E 558

Query: 555 FGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMP 576
            GK HK+ + +L  NKDI ELRNRVEAFA  + MP
Sbjct: 559 HGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

BLAST of HG10013355 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 765.8 bits (1976), Expect = 2.5e-221
Identity = 372/532 (69.92%), Postives = 439/532 (82.52%), Query Frame = 0

Query: 53  EKFDLVKGVADDDS--EDEKFSLLGKPFRVKRERDANSF--LNQVAAKRVAVADEASLET 112
           E+ D V G  DDD   E+E+F LLG P ++KR R  NS+   +    KR  V  ++ +E+
Sbjct: 72  EEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVV--DSGIES 131

Query: 113 RRVSVRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKY 172
           RR  VRAWGNQ I  ADPEIH  M KE+QRQ++GIEL+ASENFVCRAVMEALGSHLTNKY
Sbjct: 132 RRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKY 191

Query: 173 SEGMPGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLL 232
           SEGMPGARYYTGNQYID+IE+LC  RALAAF L+ EKWGVNVQPYSCTSANFAV+TGLL+
Sbjct: 192 SEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLM 251

Query: 233 PKDRIMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALD 292
           P +RIMGLDS SGGH+SHGYY+P GKK+S  SIFFES PY+V+P TGY+DYDKLEEKALD
Sbjct: 252 PGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALD 311

Query: 293 YRPKILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVT 352
           YRPKILICGGSSYPR+W++ R R IA+KCGA+LM DMA ISGLVAAKE  +PF+YCDIVT
Sbjct: 312 YRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVT 371

Query: 353 STTHKSLRGPRGGIIFFRRGLKSRKQGV-LTHGDDTSTYDFEDRINFSVFPSLQGGPHNN 412
           STTHKSLRGPRGGIIF++RGLK +KQ + L H +    YDFE++INFSVFPSLQGGPHNN
Sbjct: 372 STTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNN 431

Query: 413 HIAALAIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLG 472
           HIAALAIALKQ ASPEY++Y++QVKKNA+ALASAL+ RKCKL+T GTDNHL+LWDLTPLG
Sbjct: 432 HIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLG 491

Query: 473 LTAKDYEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLK 532
           LT K YE +CE CHITVNK AI+ +NG ISPGGVRIG+PAMT+RGCLE +FET+ D L +
Sbjct: 492 LTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYR 551

Query: 533 ATQITKIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
           A QI     R  + GKL K+ + ++ + K+I +LRN+VEAFA  FAMP FD+
Sbjct: 552 AAQIASAAQR--EHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of HG10013355 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 610.1 bits (1572), Expect = 1.8e-174
Identity = 292/465 (62.80%), Postives = 365/465 (78.49%), Query Frame = 0

Query: 113 VRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  +   DPEIH+++ KE++RQ +GIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDR 232
           PG RYY GN++IDEIE LC +RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYY+  GKKISATSI+FESLPY+VN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTH 352
           +LICGGS+YPR+WDYAR R IA+K GA+L+CDMAHISGLVAA+E  +PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAAL 412
           KSLRGPR G+IF+R+G K  K+G      + + YDFED+INF+VFP+LQGGPHN+ I AL
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG----QPEGAVYDFEDKINFAVFPALQGGPHNHQIGAL 303

Query: 413 AIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKD 472
           A+ALKQ  +P ++VY +QVK NA AL + L+ +  ++VTNGT+NHLVLWDL PLGLT   
Sbjct: 304 AVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNK 363

Query: 473 YEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQIT 532
            E +C+ C IT+NK A++GD+ A++PGGVRIG PAMT+RG +E DFE I + L +A  +T
Sbjct: 364 VEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLT 423

Query: 533 KIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGF 578
             +  +  +GKL KD    L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 LDI--QKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

BLAST of HG10013355 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 590.1 bits (1520), Expect = 1.9e-168
Identity = 282/467 (60.39%), Postives = 355/467 (76.02%), Query Frame = 0

Query: 113 VRAWGNQPIRIADPEIHNIMAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  +   DPEI++++ KE+ RQ +GIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQYIDEIELLCCARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDR 232
           PG RYY G ++IDEIE LC +R+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYYS  GK ISATSI+FE+LPY+V+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTH 352
           ++ICGG+SYPREWDYAR R +A+K GA L+CDMAH S LVAA+E   PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAAL 412
           KSLRGPR G+IF+R+G K  K+G      +   YDF+ +IN +VFP+LQ GPHNN I AL
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG----QPEGEVYDFDAKINSAVFPALQSGPHNNKIGAL 303

Query: 413 AIALKQVASPEYRVYIQQVKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKD 472
           A+ALKQV +P ++VY +QVK NA  LAS L+ +   LVT+GTDNHL+LWDL PLGLT   
Sbjct: 304 AVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNK 363

Query: 473 YEMICEACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQIT 532
            E +CE C+IT+N+ A++GD   ++PGGVRIGTPAMT+RG +E DFE I + L +A  IT
Sbjct: 364 VEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTIT 423

Query: 533 KIVIRRGKFGKLHKDIMMNLQNNKDIVELRNRVEAFAASFAMPGFDI 580
             +  + ++GK+ KD    L NNK+I E++  VE F   F MPGF I
Sbjct: 424 LDI--QEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

BLAST of HG10013355 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 479.6 bits (1233), Expect = 3.6e-135
Identity = 256/512 (50.00%), Postives = 338/512 (66.02%), Query Frame = 0

Query: 72  SLLGKPFRVKRERDANSFLNQVAAKRVAVADEASLETRRVSVRAWGNQPIRIADPEIHNI 131
           S + KP R    R  + +++ + ++ V   DE   E  RV+     N P+   DPEI +I
Sbjct: 12  SSIDKPIR-PLIRSTSCYMSSLPSEAV---DEK--ERSRVTWPKQLNAPLEEVDPEIADI 71

Query: 132 MAKERQRQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDEIELLC 191
           +  E+ RQ+KG+EL+ SENF   +VM+A+GS +TNKYSEG PGARYY GN+YID  E LC
Sbjct: 72  IEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLC 131

Query: 192 CARALAAFDLDLEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSASGGHLSHGYYSP 251
             RAL AF LD EKWGVNVQP S + ANF VYT LL P +RIM LD   GGHLSHGY + 
Sbjct: 132 QKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTD 191

Query: 252 VGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYARCR 311
             KKISA SIFFE++PYR++  TGY+DYD++E+ A  +RPK+++ G S+Y R +DYAR R
Sbjct: 192 T-KKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIR 251

Query: 312 QIANKCGAILMCDMAHISGLVAAKECVSPFEYCDIVTSTTHKSLRGPRGGIIFFRRGLKS 371
           ++ NK  A+++ DMAHISGLVAA    SPF+Y D+VT+TTHKSLRGPRG +IFFR+G+K 
Sbjct: 252 KVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKE 311

Query: 372 -RKQGVLTHGDDTSTYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASPEYRVYIQQ 431
             KQG          YDFED+IN +VFP LQGGPHN+ I  LA+ALKQ  + EY+ Y +Q
Sbjct: 312 INKQG------KEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQ 371

Query: 432 VKKNAQALASALLRRKCKLVTNGTDNHLVLWDLTPLGLTAKDYEMICEACHITVNKCAIY 491
           V  N+   A  L+ R  +LV+ GTDNHLVL +L P G+     E + EA HI  NK  + 
Sbjct: 372 VLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVP 431

Query: 492 GDNGAISPGGVRIGTPAMTTRGCLEADFETIVDILLKATQITKIVIRRGKFGKLHKDIMM 551
           GD  A+ PGG+R+GTPA+T+RG +E DF  + +   KA  I   V    +  KL KD + 
Sbjct: 432 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKL-KDFVS 491

Query: 552 NLQNN----KDIVELRNRVEAFAASFAMPGFD 579
            ++++     +I +LR+ VE FA  F   GF+
Sbjct: 492 AMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509