HG10012778 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10012778
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	Chr01: 24185894 .. 24186613 (+)
RNA-Seq Expression	HG10012778
Synteny	HG10012778

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

mRNA sequence

ATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

Coding sequence (CDS)

ATGTGCACAATTGAGAAGCGAGGCAATTTCTTTATCCTAACTCTGACCGGCAACGACGACCACCGCCTCGGCCCATCCTTAATCAACTCCATCCTCAAAGCTCTTTCACAAGTCAAAGCACAAGCCTCCGCCGGTTCAGTTCTCATCACAACTTCCCATGGCAAATTCTTCTCAAACGGCTTTGATCTCCCCTGGGCCCTTGCCGCCGGCTCTGTTTCTGCTGCCCGTGACCGCCTAAAGCACATAATCGAGATCTTCAAGCCCCTCATAGCCGAACTCCTTTCCCTTCCTATGCCTACCATTGCTGCCATTACAGGACACGCCGCTGCCGCCGGTTTCACATTGGCGCTGAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTGCTGTACATGCGTGAGTTGGACCTGGGTCTAACTCTACCGGATTACTTTATGGCTCTGTTTCAGTCCAAGACAGGATCCAGTTTGGCTATGCGCGACCTGATCCTGAGGGGGATGAAAGTGAGAGGTGAAGCGGCAGTGAGAATGGGCATCGTGGAATCGGCGCACCACGGCCAGGACGGCGTCGTGAGCGCTGCAGTGCGGCTTGGGGAGCAGCTGGCGAGGAGAAAATGGGATGGGGAAGTTTATGCTGAAATCAGGAAGAGTTTGTATCCTGAGGTCTCTCAGGTGCTTGGGTTACTGGGGAAAGCCATATCCATTTCTAAACTATGA

Protein sequence

MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNGFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGIVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL

Homology

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BLAST of HG10012778 vs. NCBI nr
Match: XP_023526416.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.1 bits (1089), Expect = 8.1e-115
Identity = 213/239 (89.12%), Postives = 229/239 (95.82%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRLGPSLINS+LKALSQVKAQA+ GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGS+S ARDRL HII+IFKPL+A+LLSLPMPTIAAI+GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLSHIIQIFKPLVAQLLSLPMPTIAAISGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF+SK GSSLAMRDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLAMRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VES HHG+DGV +AAV+LGEQLA RKWDGEVYAEIRKS+YPEVS+VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWDGEVYAEIRKSVYPEVSEVLGLRGKAISISKL 239

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BLAST of HG10012778 vs. NCBI nr
Match: XP_022979202.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-114
Identity = 214/239 (89.54%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRLGPSLINSILKALSQVKAQA+ GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGS+S ARDRL HII+IFKPL+A+LLSLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF SK GSSLAMRDLILRGMKVRGEAAVR+G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VES HHG+DGV +AAV+LGEQLA RKWDGEVYAEIRKSLYPEVS+VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

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BLAST of HG10012778 vs. NCBI nr
Match: XP_038877355.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 420.6 bits (1080), Expect = 8.9e-114
Identity = 214/239 (89.54%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+E+RGNFFILTL GNDDHRLGPSLINSILKALSQVKAQA  GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEERGNFFILTLIGNDDHRLGPSLINSILKALSQVKAQAFPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWAL AGSVSAARDRL HII+IFKP++AELLSLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALTAGSVSAARDRLNHIIDIFKPVVAELLSLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLA+RDLILRGMKV GEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAVRDLILRGMKVGGEAAVRMGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VESAH GQD VV+AAVRLGEQLA R WDGEVYA++R+SLYPEVS+VLGL GKAISISKL
Sbjct: 181 VESAHQGQDAVVNAAVRLGEQLAGRNWDGEVYAKVRRSLYPEVSEVLGLTGKAISISKL 239

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BLAST of HG10012778 vs. NCBI nr
Match: KAG6582371.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 420.2 bits (1079), Expect = 1.2e-113
Identity = 212/239 (88.70%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRLGPSLINSILKALSQVKAQA++GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGS+S ARDRL HII+IFKPL+A+LLSLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF+SK GSSLA RDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VES HHG+DGV +AAV+LGEQLA RKW+GEVYAEIRKSLYPEVS+VLGL GK ISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239

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BLAST of HG10012778 vs. NCBI nr
Match: XP_004134079.1 (enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypothetical protein Csa_010206 [Cucumis sativus])

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-112
Identity = 214/239 (89.54%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWAL+AGSVSAAR RL H+IEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+SAH G+D VV+AAVRLGEQLA R WDGEVYAEIRKSLYPEVS+VLGL GK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

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BLAST of HG10012778 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.8e-78
Identity = 145/240 (60.42%), Postives = 187/240 (77.92%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG+ +HR  P  I +IL  L Q K+Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDL WA  AGS + A +RL  ++E FKP++A LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K G+S A R+L+L G K+RGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+S A+  ++GVV A+VRLGE+LA +KW GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of HG10012778 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.7e-73
Identity = 139/240 (57.92%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG D+HR  P  I S+L  L Q K+Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDL WA +AG    A  R+  +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K GS +A R+L+L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+S AH   +GVV A V LGE LA +KW+GEVYA IRKSLYPE+ +++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

[Close]

BLAST of HG10012778 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.2e-54
Identity = 112/224 (50.00%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQAS-AGSVLITTSHGKFFSN 60
           MC++EKR   FIL LTG+ +HRL P+L++S+   ++Q+++  S + SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSH 120
           G+DL  A +  S+S   D         + L+A+L+SLPMPTIAA+TGHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMG 180
           DY++MR DRG LYM ELD+ L +P +FMA+ + K GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEV 224
           IV+SA+      V AA++LGE++ +R  DG VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of HG10012778 vs. ExPASy Swiss-Prot
Match: A1JK30 (Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=fadJ PE=3 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 3.4e-07
Identity = 62/230 (26.96%), Postives = 105/230 (45.65%), Query Frame = 0


Query: 9   NFFILT--LTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNGFDLPWA 68
           N  I+T  + G+  + L       I + L Q +A +    ++I +     F  G D+   
Sbjct: 34  NIGIITVDVVGDKVNTLKAKFAEQIAEILQQAQALSQLQGLVIISGKPDSFIAGADITMI 93

Query: 69  LAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALS-HDYLIMR 128
            A  +   AR     + +  + ++A++ + P+P +AAI G     G  LAL+ H  +  +
Sbjct: 94  AACHTAQDAR----ILAQKGQSILAQIAAFPVPVVAAIHGACLGGGLELALACHSRICSQ 153

Query: 129 SDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGIVESAH 188
            D+ VL + E+ LGL              G S A+ D+IL G +VR   A++MG+V+   
Sbjct: 154 DDKTVLGLPEVQLGLLPGSGGTQRLPRLVGVSKAL-DMILTGRQVRARQALKMGLVDDV- 213

Query: 189 HGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVL-GLLGKAI 235
             QD ++  A+    Q A+  W       + K   P   ++L G LGKA+
Sbjct: 214 VPQDILLDVAI----QRAKAGW-------LDKPALPWQERLLSGPLGKAL 246

[Close]

BLAST of HG10012778 vs. ExPASy Swiss-Prot
Match: A9MR28 (Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=caiD PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.4e-06
Identity = 50/161 (31.06%), Postives = 76/161 (47.20%), Query Frame = 0


Query: 48  LITTSHGKFFSNGFDLPWALAAGSVSAARDRLKHIIEIFKP----LIAELLSLPMPTIAA 107
           +IT +  KFFS G+DL  A A G    A          F P     + EL  L  P IAA
Sbjct: 51  IITGAGEKFFSAGWDLK-AAAEGEAPDAD---------FGPGGFAGLTELFDLNKPVIAA 110

Query: 108 ITGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDL 167
           + G+A   GF LAL+ D++I  +D     + E  LG+ +PD    L   K      + D+
Sbjct: 111 VNGYAFGGGFELALAADFIIC-ADHASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNDM 170

Query: 168 ILRGMKVRGEAAVRMGIVESAHHGQDGVVSAAVRLGEQLAR 205
           ++ G ++  E A+R G+V       + ++ +A  L  QL +
Sbjct: 171 VMTGRRMTAEEALRWGVVNRVVSPHE-LLDSARELARQLVQ 198

[Close]

BLAST of HG10012778 vs. ExPASy TrEMBL
Match: A0A6J1IN54 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479008 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.1e-115
Identity = 214/239 (89.54%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRLGPSLINSILKALSQVKAQA+ GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGS+S ARDRL HII+IFKPL+A+LLSLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF SK GSSLAMRDLILRGMKVRGEAAVR+G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VES HHG+DGV +AAV+LGEQLA RKWDGEVYAEIRKSLYPEVS+VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

[Close]

BLAST of HG10012778 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.2e-113
Identity = 214/239 (89.54%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWAL+AGSVSAAR RL H+IEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+SAH G+D VV+AAVRLGEQLA R WDGEVYAEIRKSLYPEVS+VLGL GK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of HG10012778 vs. ExPASy TrEMBL
Match: A0A1S3AWB3 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483618 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.1e-113
Identity = 214/239 (89.54%), Postives = 225/239 (94.14%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGSVSAAR RL HIIEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+SAHH +  VV+AAVRLGEQL  R WDGEVYAEIRKSLYPE+S+VLGL GKAISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of HG10012778 vs. ExPASy TrEMBL
Match: A0A5D3CZF5 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001370 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-112
Identity = 213/239 (89.12%), Postives = 225/239 (94.14%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRL PSLINSILKALSQVKAQAS  SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGSVSAAR R+ HIIEIFK ++AEL+SLPMPTIAAITGHAAAAGFTLALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRINHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALF+SK GSSLAMRDLILRGMKVRGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+SAHH +  VV+AAVRLGEQL  R WDGEVYAEIRKSLYPE+S+VLGL GKAISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of HG10012778 vs. ExPASy TrEMBL
Match: A0A6J1E8J9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431761 PE=4 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.8e-112
Identity = 209/239 (87.45%), Postives = 225/239 (94.14%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRGNFFILTLTG+DDHRLGPSLINS+LKALSQVKAQA+ GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDLPWALAAGS+S ARDRL HII+IFKPL+A+LLSLPMPTIAAI GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF+SK GSSLA RDLILRGMKVRGEAAVRMG+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           VES HHG+DGV +AAV+LGEQLA RKW+GEVYAEIRKSLYPEVS+VLGL GK I IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

[Close]

BLAST of HG10012778 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 292.4 bits (747), Expect = 3.4e-79
Identity = 145/240 (60.42%), Postives = 187/240 (77.92%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG+ +HR  P  I +IL  L Q K+Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDL WA  AGS + A +RL  ++E FKP++A LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K G+S A R+L+L G K+RGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+S A+  ++GVV A+VRLGE+LA +KW GEVYA IRKSLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of HG10012778 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 276.6 bits (706), Expect = 1.9e-74
Identity = 139/240 (57.92%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQASAGSVLITTSHGKFFSNG 60
           MCT+EKRG+ F+LTLTG D+HR  P  I S+L  L Q K+Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSHD 120
           FDL WA +AG    A  R+  +++ FKP++A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMGI 180
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K GS +A R+L+L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEVSQVLGLLGKAISISKL 240
           V+S AH   +GVV A V LGE LA +KW+GEVYA IRKSLYPE+ +++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

[Close]

BLAST of HG10012778 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-55
Identity = 112/224 (50.00%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTIEKRGNFFILTLTGNDDHRLGPSLINSILKALSQVKAQAS-AGSVLITTSHGKFFSN 60
           MC++EKR   FIL LTG+ +HRL P+L++S+   ++Q+++  S + SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSVSAARDRLKHIIEIFKPLIAELLSLPMPTIAAITGHAAAAGFTLALSH 120
           G+DL  A +  S+S   D         + L+A+L+SLPMPTIAA+TGHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFQSKTGSSLAMRDLILRGMKVRGEAAVRMG 180
           DY++MR DRG LYM ELD+ L +P +FMA+ + K GS  A RD++L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHHGQDGVVSAAVRLGEQLARRKWDGEVYAEIRKSLYPEV 224
           IV+SA+      V AA++LGE++ +R  DG VY ++R+SL  EV
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:

BLAST of HG10012778 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526416.1	8.1e-115	89.12	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979202.1	1.1e-114	89.54	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima]	[more]
XP_038877355.1	8.9e-114	89.54	enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida]	[more]
KAG6582371.1	1.2e-113	88.70	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004134079.1	1.3e-112	89.54	enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypotheti...	[more]

BLAST of HG10012778 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	4.8e-78	60.42	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	2.7e-73	57.92	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	2.2e-54	50.00	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
A1JK30	3.4e-07	26.96	Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O...	[more]
A9MR28	6.4e-06	31.06	Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-...	[more]

BLAST of HG10012778 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IN54	5.1e-115	89.54	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A0A0L9X4	6.2e-113	89.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1	[more]
A0A1S3AWB3	8.1e-113	89.54	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5D3CZF5	1.4e-112	89.12	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1E8J9	1.8e-112	87.45	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of HG10012778 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	3.4e-79	60.42	indole-3-butyric acid response 10	[more]
AT4G14440.1	1.9e-74	57.92	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	1.5e-55	50.00	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 1..227 e-value: 3.6E-36 score: 126.6
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..232
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..232
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 9.95054E-21 score: 84.5344
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 25..206 e-value: 1.0E-21 score: 77.4
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10012778.1	HG10012778.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity
molecular_function	GO:0003824	catalytic activity