HG10012777 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10012777
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	Chr01: 24183921 .. 24184640 (+)
RNA-Seq Expression	HG10012777
Synteny	HG10012777

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

mRNA sequence

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Coding sequence (CDS)

ATGTGCACACTCGAGAAGCGACGCAATCTTTTCATCCTAACTCTGACCGGCGACGACGACCACCGCCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAGGCTCTTTCCAAAGTCAAATCCCAAGCCTCCCGTGGCTCCGTTCTCATCACTACTTCCCATGGCAAATTCTTCTCTAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCCGAACGCCTCAGGCATATGGTCGAGATTTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTCCCCATGCCCACCATCGCTGCCATTGCAGGCCACGCCGCCGCGGCCGGCTTCATGTTAGCCCTCAGTCACGACTATTTGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCACGGCGCTGTTTAAATCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGCTCTTGAGGGGAATGAAAGTGAAAGGTGAAGCGGCGGTGAGGATGGGGATCGTGGAATCGGCGCATGACGGAGAGGACGGTGTCATGAAGGCTGCCGTACGACTCGGGGAGCAGTTGGGAGCGAGAAATTGGAACGGCGAACCCTACGCAGAAATTAGGAAAAGTCTTCATCCAGAGATCTCTGGGCTGCTTGGACTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGA

Protein sequence

MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNGFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHDYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGIVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL

Homology

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BLAST of HG10012777 vs. NCBI nr
Match: XP_038877347.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 432.6 bits (1111), Expect = 2.3e-117
Identity = 225/239 (94.14%), Postives = 230/239 (96.23%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAA+RL HMV IFKP+VA+LLSLPMPTIAAIAGHAAAAGF LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAADRLMHMVHIFKPIVAELLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YLFMR D GVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI
Sbjct: 121 YLFMRRDLGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VES HDGEDGVM+AAVR GEQLGARNWNGEPYAEIRKSL+PEIS LLGLPEKVISISKL
Sbjct: 181 VESVHDGEDGVMEAAVRFGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKVISISKL 239

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BLAST of HG10012777 vs. NCBI nr
Match: XP_022924233.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 417.2 bits (1071), Expect = 9.8e-113
Identity = 216/239 (90.38%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGDD+HRLSPSFIASIL+ALSKVKS+AS GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLS PMPTIAAIAGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGL+LPDYF+ALFKSKIGSSSVRRDVLL GMKV GE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVTGEPAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VESAH+G DGVMKAAVRLGEQL  RNWNGEPYAEIRKSLHPEIS LLGLPEKV SISKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

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BLAST of HG10012777 vs. NCBI nr
Match: KAG6582370.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417.2 bits (1071), Expect = 9.8e-113
Identity = 215/239 (89.96%), Postives = 227/239 (94.98%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGDD+HRLSPS+IASIL+ALSKVK++AS GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSYIASILKALSKVKAEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLS PMPTIAAIAGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGL+LPDYF+ALFKSKIGSSSVRRDVLL GMKVKGE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVKGEPAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VESAH+G DGVMKAAVRLGEQL  RNWNGEPYAEIRKSLHPEIS LLGLPEKV SISKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

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BLAST of HG10012777 vs. NCBI nr
Match: XP_004134080.1 (enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56882.1 hypothetical protein Csa_009715 [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 1.7e-112
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

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BLAST of HG10012777 vs. NCBI nr
Match: XP_023526034.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.0 bits (1068), Expect = 2.2e-112
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGDD+HRLSPS+IASIL+ALSKVKS+AS GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSYIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLS PMPTIAAIAGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGL+LPDYF+ALFKSKIGSSSVRRDVLL GMKVKGE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVKGEPAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VESAH+G DGVMKAAVRLGEQL  R WNGEPYAEIRKSLHPEIS LLGLPEKV SISKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERKWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

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BLAST of HG10012777 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.4e-85
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of HG10012777 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 6.3e-78
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of HG10012777 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 5.9e-52
Identity = 110/224 (49.11%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQAS-RGSVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S        M    + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVV------MDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of HG10012777 vs. ExPASy Swiss-Prot
Match: A1JK30 (Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=fadJ PE=3 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 59/223 (26.46%), Postives = 105/223 (47.09%), Query Frame = 0


Query: 9   NLFILT--LTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNGFDLSWA 68
           N+ I+T  + GD  + L   F   I E L + ++ +    ++I +     F  G D++  
Sbjct: 34  NIGIITVDVVGDKVNTLKAKFAEQIAEILQQAQALSQLQGLVIISGKPDSFIAGADITMI 93

Query: 69  QAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALS-HDYLFMR 128
            A  +    A+  R + +  + ++AQ+ + P+P +AAI G     G  LAL+ H  +  +
Sbjct: 94  AACHT----AQDARILAQKGQSILAQIAAFPVPVVAAIHGACLGGGLELALACHSRICSQ 153

Query: 129 SDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGIVESAH 188
            D+ VL + EV LGL      T      +G S    D++L G +V+   A++MG+V+   
Sbjct: 154 DDKTVLGLPEVQLGLLPGSGGTQRLPRLVGVSKA-LDMILTGRQVRARQALKMGLVDDVV 213

Query: 189 DGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLG 229
             +D ++  A+    Q     W  +P    ++ L   +SG LG
Sbjct: 214 P-QDILLDVAI----QRAKAGWLDKPALPWQERL---LSGPLG 243

[Close]

BLAST of HG10012777 vs. ExPASy Swiss-Prot
Match: A4TM82 (Fatty acid oxidation complex subunit alpha OS=Yersinia pestis (strain Pestoides F) OX=386656 GN=fadJ PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.4e-06
Identity = 47/177 (26.55%), Postives = 81/177 (45.76%), Query Frame = 0


Query: 9   NLFILTL--TGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNGFDLSWA 68
           N+ I+T+   GD  + L   F   I   L +  +      ++I +     F  G D++  
Sbjct: 49  NIGIITIDVVGDKVNTLKAEFADQIATILQQAHALPKLQGLVIVSGKPDSFIAGADITMI 108

Query: 69  QAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALS-HDYLFMR 128
            A       A   R + +  + ++AQ+ + P+P +AAI G     G  LAL+ H  +   
Sbjct: 109 AAC----RTAHDARVLAQKGQSILAQIAAFPVPVVAAIHGACLGGGLELALACHSRICSL 168

Query: 129 SDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGIVE 183
            D+ VL + EV LGL      T      +G S    D++L G +++   A++MG+V+
Sbjct: 169 DDKTVLGLPEVQLGLLPGSGGTQRLPRLVGVSKA-LDMILTGKQIRPRQALKMGLVD 220

[Close]

BLAST of HG10012777 vs. ExPASy TrEMBL
Match: A0A6J1EBT7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431760 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 4.8e-113
Identity = 216/239 (90.38%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGDD+HRLSPSFIASIL+ALSKVKS+AS GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLS PMPTIAAIAGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGL+LPDYF+ALFKSKIGSSSVRRDVLL GMKV GE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVTGEPAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VESAH+G DGVMKAAVRLGEQL  RNWNGEPYAEIRKSLHPEIS LLGLPEKV SISKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

[Close]

BLAST of HG10012777 vs. ExPASy TrEMBL
Match: A0A0A0L8C7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.1e-113
Identity = 215/239 (89.96%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTG DDHR SPS IA+ILEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL HMV+IFKPVVA+LLSLPMPTIAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSLPMPTIAAISGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SAHDGE+GVM+AAVRLGEQLG R WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of HG10012777 vs. ExPASy TrEMBL
Match: A0A1S3AWS8 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483623 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.9e-112
Identity = 213/239 (89.12%), Postives = 226/239 (94.56%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTGD DHRL+PS IA++LEALSKVKSQAS GSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWA AAGSVSAAAERL HMV+ FKPV+A+LLSLPMPTIAAI+GHAAAAGFMLALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKIGSSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +SA+DGE GVM+AAVRLGEQLGAR WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of HG10012777 vs. ExPASy TrEMBL
Match: A0A6J1IWE7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479854 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.2e-111
Identity = 212/239 (88.70%), Postives = 228/239 (95.40%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILT+TGDD+HRLSPSFIASIL+ALSKVKS+AS GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTITGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWAQAAGSVSAAAERL +MV+IFKPVVA+LLSLPMPTIAAIAGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHYMVQIFKPVVAELLSLPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+ MRSDRGVLYMSEVDLGL+LPDYF+ALFKSKIGSSSVRRD+LL GMKVKGE+AVRMGI
Sbjct: 121 YMIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDLLLTGMKVKGESAVRMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           VESAH+G DGVMKAAVRLGEQL  RNWNGEPYAEIRKSL+PEIS LLGLPEK  SISKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLYPEISRLLGLPEKFFSISKL 239

[Close]

BLAST of HG10012777 vs. ExPASy TrEMBL
Match: A0A5A7U6I8 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001360 PE=4 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.6e-111
Identity = 211/239 (88.28%), Postives = 225/239 (94.14%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKRRNLFILTLTGD DHRL+PS IA++LEALSKVKSQAS GSVL+TTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLVTTSHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDLSWA AAGSVSAAAERL HMV+ FKPV+A+LLSLPMPTIAAI+GHAAAAGFMLALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           YL MRSDRGVLYMSEVDLGLSLPDYF ALFKSKIGSSSVRRDVL+RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
            +S +DGE GVM+AAVRLGEQLGAR WNGEPYAEIRKSL+PEIS LLGLPEK ISISKL
Sbjct: 181 ADSVYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of HG10012777 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 317.4 bits (812), Expect = 9.9e-87
Identity = 159/240 (66.25%), Postives = 199/240 (82.92%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTGD +HR  P  IA+IL  L + KSQ++RGS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ AGS + AA RL  MVE FKPVVA LL LPMPTIAA+ GHAAAAG +LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GLS+PDYF+AL ++KIG+S+ RR++LL G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGLPEKVISISKL 240
           V+S A+D E+GV+ A+VRLGE+L A+ W+GE YA IRKSL+PE+ G+LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of HG10012777 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 292.0 bits (746), Expect = 4.5e-79
Identity = 147/230 (63.91%), Postives = 183/230 (79.57%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASRGSVLITTSHGKFFSNG 60
           MCTLEKR +LF+LTLTG+D+HR  P  IAS+L  L + KSQ+++GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSHD 120
           FDL+WAQ+AG    A +R+  MV+ FKPV+A LL LPMPTIAA+ GHAAA+G M ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180
           Y+FMR DRGVLYMSEVD+GL +PDYF+AL  +K+GS   RR++LL G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEISGLLGL 230
           V+S AHD  +GV++A V LGE L A+ WNGE YA IRKSL+PE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of HG10012777 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 205.7 bits (522), Expect = 4.2e-53
Identity = 110/224 (49.11%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQAS-RGSVLITTSHGKFFSN 60
           MC+LEKR  LFIL LTGD +HRL+P+ + S+   +++++S  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLSWAQAAGSVSAAAERLRHMVEIFKPVVAQLLSLPMPTIAAIAGHAAAAGFMLALSH 120
           G+DL+ A++  S+S        M    + +VA L+SLPMPTIAA+ GHA+AAG +LA+SH
Sbjct: 61  GYDLALAESNPSLSVV------MDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLFMRSDRGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMG 180
           DY+ MR DRG LYMSE+D+ L +P +F A+ + KIGS + RRDV+L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGEDGVMKAAVRLGEQLGARNWNGEPYAEIRKSLHPEI 224
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:

BLAST of HG10012777 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877347.1	2.3e-117	94.14	enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida]	[more]
XP_022924233.1	9.8e-113	90.38	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata]	[more]
KAG6582370.1	9.8e-113	89.96	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004134080.1	1.7e-112	89.96	enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56882.1 hypotheti...	[more]
XP_023526034.1	2.2e-112	89.96	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10012777 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	1.4e-85	66.25	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	6.3e-78	63.91	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	5.9e-52	49.11	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
A1JK30	2.3e-08	26.46	Fatty acid oxidation complex subunit alpha OS=Yersinia enterocolitica serotype O...	[more]
A4TM82	6.4e-06	26.55	Fatty acid oxidation complex subunit alpha OS=Yersinia pestis (strain Pestoides ...	[more]

BLAST of HG10012777 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBT7	4.8e-113	90.38	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0L8C7	8.1e-113	89.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141780 PE=4 SV=1	[more]
A0A1S3AWS8	6.9e-112	89.12	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1IWE7	1.2e-111	88.70	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5A7U6I8	2.6e-111	88.28	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of HG10012777 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	9.9e-87	66.25	indole-3-butyric acid response 10	[more]
AT4G14440.1	4.5e-79	63.91	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	4.2e-53	49.11	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 23..203 e-value: 6.1E-21 score: 74.9
None	No IPR available	GENE3D	3.90.226.10		coord: 1..224 e-value: 5.6E-37 score: 129.2
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..232
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..232
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 1.77128E-22 score: 89.1568
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10012777.1	HG10012777.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity
molecular_function	GO:0003824	catalytic activity