HG10012704 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10012704 vs. NCBI nr
Match: XP_038882302.1 (vicilin Car i 2.0101 [Benincasa hispida])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 715/856 (83.53%), Postives = 751/856 (87.73%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAA 60
           MALS VKFRLCL+AFT+FLACVSVGLG EGESLGS VGADNGCVNGCEELKGKNVDEFAA
Sbjct: 1   MALSTVKFRLCLVAFTLFLACVSVGLGAEGESLGSGVGADNGCVNGCEELKGKNVDEFAA 60

Query: 61  CAKRCGVNQKVSEICRQWCQVAEKHGAEQQRRCRQACEERLGEQERGEDVEEYKRRDPER 120
           CAK+CGVNQKV+EICRQWCQVAEKHGAEQQRRCRQACEERL EQERGEDVEEYKRRDPER
Sbjct: 61  CAKKCGVNQKVTEICRQWCQVAEKHGAEQQRRCRQACEERLREQERGEDVEEYKRRDPER 120

Query: 121 EREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRRE 180
           EREEQRRREHEREERRRRE EREHERGRG RDENERDPE EREERRREEQRRE EQ RRE
Sbjct: 121 EREEQRRREHEREERRRREREREHERGRGHRDENERDPEHEREERRREEQRREQEQHRRE 180

Query: 181 Q------------------------------ERRERERRGEREDE-DENQRDPDWRREQE 240
           Q                              ERRER+RR ER+DE DENQRDPD RREQE
Sbjct: 181 QERRGKREDEDDENQRDPDWRREQERREQERERRERQRREERDDEDDENQRDPDRRREQE 240

Query: 241 RREQERRRREQEQERRERQRRGERE----------------------------------- 300
           RREQERRRREQE+ERRERQ++GERE                                   
Sbjct: 241 RREQERRRREQERERRERQQKGEREVEDDENQRDPDWRREQERREQERERRERQRREERD 300

Query: 301 -DEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERR 360
            ++DENQRDPDWRREQERREQERRRREQE+E RERQRRGER+DED NQRDPDWR+EQERR
Sbjct: 301 DEDDENQRDPDWRREQERREQERRRREQEREGRERQRRGEREDEDGNQRDPDWRREQERR 360

Query: 361 EQERRRREQEQREREWEREHGRR--RDENQRGQGGREEQRSKEEERKREERQRERQPGGR 420
           EQERRRREQEQREREWEREHGRR  RDENQRGQGGREEQRS+EEERKREERQRERQ GGR
Sbjct: 361 EQERRRREQEQREREWEREHGRRGSRDENQRGQGGREEQRSREEERKREERQRERQHGGR 420

Query: 421 SRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLA 480
           SR+NQ E R TEQEQ +NPYYFQE++FQSRFRSDEGHWRVLERFSER+ELLKGIKNQR A
Sbjct: 421 SRVNQVETRWTEQEQRNNPYYFQERQFQSRFRSDEGHWRVLERFSERSELLKGIKNQRFA 480

Query: 481 VLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTT 540
           +LEARPQ+FIIPHHLDAESVL VVRGRATITT+VQ+KRETRKESY VERGDV+TIPAGTT
Sbjct: 481 ILEARPQSFIIPHHLDAESVLIVVRGRATITTVVQQKRETRKESYKVERGDVMTIPAGTT 540

Query: 541 VYLANQENEDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLE 600
           VYLANQENEDLQI KLIQPVN PGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLE
Sbjct: 541 VYLANQENEDLQIAKLIQPVNKPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLE 600

Query: 601 RIFKQKSERRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFE 660
           RIFKQKSE RGKI+RASQEQLR LSQRATS+R+GG+GARA+IKLESQTPVYSNQYGQMFE
Sbjct: 601 RIFKQKSEMRGKIVRASQEQLRGLSQRATSVRRGGQGARAVIKLESQTPVYSNQYGQMFE 660

Query: 661 ACPEEFPQLRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQW 720
           ACP+EF QLRRTDVATA+LDIKQGGMMVPHFNSRATWVVF+SEGTGSFEMGCPHIQGGQW
Sbjct: 661 ACPDEFTQLRRTDVATAVLDIKQGGMMVPHFNSRATWVVFISEGTGSFEMGCPHIQGGQW 720

Query: 721 QRGRREEERHWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLV 780
           QR RREEERH RRE     EERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLV
Sbjct: 721 QRERREEERHGRRE-----EERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLV 780

Query: 781 GFGINAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGH 788
           GFGINAENNQRNFLAGRE+IMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGG 
Sbjct: 781 GFGINAENNQRNFLAGRENIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGR 840

BLAST of HG10012704 vs. NCBI nr
Match: TYK17213.1 (vicilin [Cucumis melo var. makuwa])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 679/791 (85.84%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAA 60
           MA SKVKFRLCLLA T+FLACVSVGLG EGESLGS VG DNGCVNGC+ELKGKN+DE+AA
Sbjct: 1   MAFSKVKFRLCLLALTLFLACVSVGLGAEGESLGSGVGVDNGCVNGCQELKGKNLDEYAA 60

Query: 61  CAKRCGVNQKVSEICRQWCQVAEKHGAE-QQRRCRQACEERLGEQERGEDVEEYKRRDPE 120
           C KRCGVNQ+V+EICRQWCQV E HG E QQRRCRQ CEERL +Q++GEDVE+ K RDPE
Sbjct: 61  CEKRCGVNQEVTEICRQWCQVMEPHGGEQQQRRCRQECEERLRDQKQGEDVED-KWRDPE 120

Query: 121 REREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRR 180
           REREEQRRREHEREERRRRE ERE ERGRG RDEN+RDPEREREERRREEQRRE EQRRR
Sbjct: 121 REREEQRRREHEREERRRRERERERERGRGHRDENDRDPEREREERRREEQRREQEQRRR 180

Query: 181 EQERRERERRGEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDEN 240
           EQERRERERRGEREDE+ENQR PDWRREQERREQERRRREQE+ERRER+RRGEREDE+EN
Sbjct: 181 EQERRERERRGEREDEEENQRGPDWRREQERREQERRRREQERERRERERRGEREDEEEN 240

Query: 241 QRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERREQERRR 300
           QRDPD RREQERREQERRRREQEQERRER+RRGER+DE+ENQRDPD R+EQERREQERRR
Sbjct: 241 QRDPDRRREQERREQERRRREQEQERRERERRGEREDEEENQRDPDRRREQERREQERRR 300

Query: 301 REQEQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGRSRINQFEP 360
           REQEQREREWEREHGR   + +R +GG+EE+ S++EE +REER+RER+ GGRSR N+   
Sbjct: 301 REQEQREREWEREHGRGGSQERR-RGGQEEEISRKEEWEREERRREREHGGRSRANEVNS 360

Query: 361 RRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQT 420
           R TEQEQSHNPYYFQ+++FQSRFRSD+G WRVLERFSER+ELL+G+KNQRLA+LEARPQT
Sbjct: 361 RWTEQEQSHNPYYFQDRQFQSRFRSDQGQWRVLERFSERSELLRGLKNQRLAILEARPQT 420

Query: 421 FIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQEN 480
           FIIPHH+DAESVLFVV+GRATITTIVQE++ETRKESYNVERGDV+TIPAGTTVYLANQEN
Sbjct: 421 FIIPHHIDAESVLFVVKGRATITTIVQERKETRKESYNVERGDVVTIPAGTTVYLANQEN 480

Query: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSE 540
           EDLQIVKLIQPVNNPGEFKDYLSGGGEAQ+YYSVFS+DVLEA LNIPRDRLERIFKQ+ E
Sbjct: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQSYYSVFSDDVLEATLNIPRDRLERIFKQRGE 540

Query: 541 RRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQ 600
           RRGKIIRASQEQL+ALSQRATS++KGG+GAR+LIKLESQ+PVY+NQYGQM+EACP+EFPQ
Sbjct: 541 RRGKIIRASQEQLKALSQRATSVKKGGRGARSLIKLESQSPVYNNQYGQMYEACPDEFPQ 600

Query: 601 LRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEE 660
           LRRTDVAT++LDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPH+QG QWQRGRREEE
Sbjct: 601 LRRTDVATSVLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHVQGSQWQRGRREEE 660

Query: 661 RHWRREEERE-REERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720
           R WRREEERE  +ERS RIER+AGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE
Sbjct: 661 RQWRREEERELSDERSDRIERIAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720

Query: 721 NNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRS--TE 780
           NN+RNFLAGRE+IMNEVDREAKEL FNVEGKQAEE FKSQKESFFTEGPEGG RRS  TE
Sbjct: 721 NNKRNFLAGRENIMNEVDREAKELTFNVEGKQAEETFKSQKESFFTEGPEGGRRRSTETE 780

Query: 781 RIPLLSILKVA 788
           R PL SILK+A
Sbjct: 781 RTPLFSILKLA 789

BLAST of HG10012704 vs. NCBI nr
Match: XP_031737745.1 (vicilin Car i 2.0101 [Cucumis sativus])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 673/825 (81.58%), Postives = 732/825 (88.73%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAA 60
           MA SKVKFRLCLLAFT+FLACVSVGLG EGESLGS VG DNGCVNGC+ELKGKN+DE+AA
Sbjct: 1   MAFSKVKFRLCLLAFTLFLACVSVGLGAEGESLGSGVGVDNGCVNGCQELKGKNLDEYAA 60

Query: 61  CAKRCGVNQKVSEICRQWCQVAEKHGAEQQRRCRQACEERLGEQERGEDVEEYKRRDPER 120
           C K+CGVNQK +EICRQWCQV +  G E+QRRC+Q CEERL +QE+GEDVE+ K RDPER
Sbjct: 61  CEKQCGVNQKETEICRQWCQVMKPQGGEEQRRCQQECEERLRDQEQGEDVED-KWRDPER 120

Query: 121 EREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRRE 180
           EREEQRRREHEREERRRRE ERE ERGRGRRDEN+RDPEREREERRREEQRRE EQRRRE
Sbjct: 121 EREEQRRREHEREERRRRERERERERGRGRRDENDRDPEREREERRREEQRREQEQRRRE 180

Query: 181 QERRERERRGEREDED-ENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDEN 240
           QE+RERERRG RE+ED ENQR PDWR+EQERREQERRRRE EQERRERQRRGEREDE+EN
Sbjct: 181 QEQRERERRGRREEEDEENQRGPDWRKEQERREQERRRRE-EQERRERQRRGEREDEEEN 240

Query: 241 QRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE-------- 300
           QR PDWRREQERREQERRRRE+EQERRERQRRGER+DE+ENQR PDWR+EQE        
Sbjct: 241 QRGPDWRREQERREQERRRREEEQERRERQRRGEREDEEENQRGPDWRREQERREQERRR 300

Query: 301 ----------------------------RREQERRRREQEQREREWEREHGRRRDENQRG 360
                                       RREQERRRRE+EQREREWEREHGRR  + +R 
Sbjct: 301 REEEQERRERQRRGEREDEEENQRGPDWRREQERRRREEEQREREWEREHGRRGSQERR- 360

Query: 361 QGGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRFR 420
           +GG+EE+ S+ EE        ERQ GGRSR NQ + R TEQEQSHNPYYFQE++FQSRFR
Sbjct: 361 RGGQEEEISRREE-------SERQHGGRSRANQVKTRWTEQEQSHNPYYFQERQFQSRFR 420

Query: 421 SDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATITT 480
           SD+G WRVLERFSER+ELLKG+KNQRLA+LEARPQTFIIPHH+DAESVL VV+GRATITT
Sbjct: 421 SDQGEWRVLERFSERSELLKGLKNQRLAILEARPQTFIIPHHIDAESVLLVVKGRATITT 480

Query: 481 IVQEKRETRKESYNVERGDVITIPAGTTVYLANQENEDLQIVKLIQPVNNPGEFKDYLSG 540
           IVQEK+ETRKESYNVE GDVITIPAGTTVYLANQENE+LQIVKLIQP+NNPGEFKDYLSG
Sbjct: 481 IVQEKKETRKESYNVECGDVITIPAGTTVYLANQENEELQIVKLIQPINNPGEFKDYLSG 540

Query: 541 GGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQRATSIR 600
           GGE+QAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRAS+EQL+ALSQRATS++
Sbjct: 541 GGESQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASREQLKALSQRATSVK 600

Query: 601 KGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVPHFN 660
           KGG+GARA+IKLESQ+PVY+NQYGQM+EACP+EFPQLRRTDVAT++LDIKQGGMMVPHFN
Sbjct: 601 KGGQGARAVIKLESQSPVYNNQYGQMYEACPDEFPQLRRTDVATSVLDIKQGGMMVPHFN 660

Query: 661 SRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEERER--EERSGRIERVA 720
           SRATWVVFVS+GTGS+EMGCPHIQG QWQRGRREEER WRREEERER  E  S RIER+A
Sbjct: 661 SRATWVVFVSKGTGSYEMGCPHIQGSQWQRGRREEERQWRREEERERSDERSSSRIERIA 720

Query: 721 GRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDREAKE 780
           GRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENN+RNFLAGRE+IMNEVDREAKE
Sbjct: 721 GRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNKRNFLAGRENIMNEVDREAKE 780

Query: 781 LAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIPLLSILKV 787
           LAFNVEGKQAEE FKSQKESFFTEGPEGG  RS ER PLLSILK+
Sbjct: 781 LAFNVEGKQAEETFKSQKESFFTEGPEGGRGRSRERTPLLSILKL 815

BLAST of HG10012704 vs. NCBI nr
Match: XP_022979465.1 (vicilin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 663/796 (83.29%), Postives = 715/796 (89.82%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGAD-NGCVNGCEELKGKNVDEFA 60
           MALSKVK RLCLLAFT+FLAC+SVGLG +GESL S  G D +GCVN CEELKGKNVDEFA
Sbjct: 1   MALSKVKLRLCLLAFTLFLACLSVGLGDKGESLSSGAGVDHDGCVNRCEELKGKNVDEFA 60

Query: 61  ACAKRCGVNQKVS-----EICRQWCQVAEKHGAEQQRRCRQACEERLGEQE--RGEDVEE 120
           AC K CGVNQ+ S     E+CR  CQVAE+ G EQQR+C Q CEERL E+E  RGEDV+E
Sbjct: 61  ACKKGCGVNQRGSPRAEYEVCRLRCQVAER-GVEQQRKCEQVCEERLREREQGRGEDVDE 120

Query: 121 YKRRDPEREREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRR 180
            +RRDPE EREEQRRREHEREERRRRE ERE ERGRGRRDENERDP+RE+EER    QRR
Sbjct: 121 VERRDPEWEREEQRRREHEREERRRRERERERERGRGRRDENERDPKREQEER----QRR 180

Query: 181 EHEQRRREQERRERERRGER-EDEDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240
           E E+RRREQE+RERERRGER E++DENQRDPDWRREQERREQERRRREQEQERRERQRRG
Sbjct: 181 EQERRRREQEQRERERRGERDEEDDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240

Query: 241 EREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE 300
            R+DEDENQRDPDWRREQERREQERRRRE+EQERRER+ RG RD EDENQRDPDW     
Sbjct: 241 GRDDEDENQRDPDWRREQERREQERRRREREQERREREHRGGRDVEDENQRDPDW----- 300

Query: 301 RREQERRRREQEQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGR 360
           RREQERRRRE+EQREREWEREHGRR         GREEQRS+E+ER+R ERQ     GGR
Sbjct: 301 RREQERRRREEEQREREWEREHGRR---------GREEQRSREDERRRHERQH----GGR 360

Query: 361 SRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLA 420
           SR+NQ   RRTEQEQS+NPYYFQEQRFQSR+RSDEGHWRVLERFSER+ELLKGIKNQRLA
Sbjct: 361 SRVNQVAIRRTEQEQSNNPYYFQEQRFQSRYRSDEGHWRVLERFSERSELLKGIKNQRLA 420

Query: 421 VLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTT 480
           +LEARP TFI+PHHLDAE VL VVRGRATITT+VQEKRETRKESYNVE GDV+TIPAGTT
Sbjct: 421 LLEARPHTFIVPHHLDAECVLLVVRGRATITTVVQEKRETRKESYNVESGDVMTIPAGTT 480

Query: 481 VYLANQENEDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLE 540
           +YLANQENEDLQIVKL+QPVNNPGEFKDYLS GGE+QAYYSVFSNDVLEAALNIPRD+LE
Sbjct: 481 LYLANQENEDLQIVKLVQPVNNPGEFKDYLSAGGESQAYYSVFSNDVLEAALNIPRDKLE 540

Query: 541 RIFKQKSERRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFE 600
           RIFKQ+ ER GKI+RASQEQLRALSQRATS+RKG +G RA IKLESQTPVY+NQYGQMFE
Sbjct: 541 RIFKQRRERGGKIVRASQEQLRALSQRATSVRKGSRGVRAPIKLESQTPVYNNQYGQMFE 600

Query: 601 ACPEEFPQLRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQW 660
           ACP+EFPQLRRTDVAT+++DIKQGGMMVPHFNSRATWVVFVSEG GSFEM CPHIQ  QW
Sbjct: 601 ACPDEFPQLRRTDVATSVVDIKQGGMMVPHFNSRATWVVFVSEGAGSFEMACPHIQSSQW 660

Query: 661 QRGRREEERHWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLV 720
           QRGRREEERHWRREEE EREERSGR ERVAGRLS+GGVLVIPAGHPIAIMASPNENLRLV
Sbjct: 661 QRGRREEERHWRREEEEEREERSGRFERVAGRLSEGGVLVIPAGHPIAIMASPNENLRLV 720

Query: 721 GFGINAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGH 780
           GFGINAENN RNFLAGRE+IMNE+DREAKELAFNVEGKQA+EIF+SQ+ESFFTEGPEGG 
Sbjct: 721 GFGINAENNHRNFLAGRENIMNELDREAKELAFNVEGKQADEIFRSQRESFFTEGPEGGR 773

Query: 781 RRSTERIPLLSILKVA 788
           RRSTER PLLSILK+A
Sbjct: 781 RRSTERSPLLSILKLA 773

BLAST of HG10012704 vs. NCBI nr
Match: XP_022956153.1 (vicilin-like [Cucurbita moschata])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 669/825 (81.09%), Postives = 722/825 (87.52%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGAD-NGCVNGCEELKGKNVDEFA 60
           MALSKVK RLCLLAFT+FLAC+SVGLG +GESL S  G D +GCVN CEELKGKNVDEFA
Sbjct: 1   MALSKVKLRLCLLAFTLFLACLSVGLGAKGESLSSGAGVDHDGCVNRCEELKGKNVDEFA 60

Query: 61  ACAKRCGVNQKVS-----EICRQWCQVAEKHGAEQQRRCRQACEERLGEQE--RGEDVEE 120
           AC K CGVNQ+ S     E+CR  CQVAE+ G EQQRRC Q CEERL E+E  RGEDV+E
Sbjct: 61  ACKKGCGVNQRGSPRAEYEVCRLRCQVAER-GVEQQRRCEQVCEERLREREQGRGEDVDE 120

Query: 121 YKRRDPEREREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRR 180
            +RRDPE EREEQRRREHEREERRRRE ERE ERGRGRRD NERDP+RE+EER    QRR
Sbjct: 121 VERRDPEWEREEQRRREHEREERRRRERERERERGRGRRDLNERDPKREQEER----QRR 180

Query: 181 EHEQRRREQERRERERRGER-EDEDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240
           E E+RRREQE+RERERRGER E++DENQRDPDWRREQERREQERRRREQEQERRERQRRG
Sbjct: 181 EQERRRREQEQRERERRGERDEEDDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240

Query: 241 EREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE 300
            R+DEDENQRDPDWRREQERREQERRRR  EQERRERQRRGERDDEDENQRDPDWR+EQE
Sbjct: 241 GRDDEDENQRDPDWRREQERREQERRRR--EQERRERQRRGERDDEDENQRDPDWRREQE 300

Query: 301 -----------------------------RREQERRRREQEQREREWEREHGRRRDENQR 360
                                        RREQERRRRE+EQREREWEREHGRR      
Sbjct: 301 RREQEQERRERERRGGRDDEDENQRDPDWRREQERRRREEEQREREWEREHGRR------ 360

Query: 361 GQGGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRF 420
              GREEQRS+E+ER+R+ERQ     GGRSR+NQ   RRTE EQS+NPYYFQEQRFQSR+
Sbjct: 361 ---GREEQRSREDERRRQERQH----GGRSRVNQVAIRRTELEQSNNPYYFQEQRFQSRY 420

Query: 421 RSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATIT 480
           RSD+GHWRVLE+FS+R+ELLKGIKNQRLA+LEARP TFI+PHHLDAE VL VVRGRATIT
Sbjct: 421 RSDQGHWRVLEKFSDRSELLKGIKNQRLAILEARPHTFIVPHHLDAECVLLVVRGRATIT 480

Query: 481 TIVQEKRETRKESYNVERGDVITIPAGTTVYLANQENEDLQIVKLIQPVNNPGEFKDYLS 540
           T+VQEKRETRKESYNVE GDV+TIPAGTT+YLANQENEDLQIVKL+QPVNNPGEFKDYLS
Sbjct: 481 TVVQEKRETRKESYNVESGDVMTIPAGTTLYLANQENEDLQIVKLVQPVNNPGEFKDYLS 540

Query: 541 GGGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQRATSI 600
            GGEAQAYYSVFSNDVLEAALNIPRD+LERIFKQ+ ER GKIIRASQEQLRALSQRATS+
Sbjct: 541 AGGEAQAYYSVFSNDVLEAALNIPRDKLERIFKQRRERGGKIIRASQEQLRALSQRATSV 600

Query: 601 RKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVPHF 660
           R+G +G RA IKLESQTPVY+NQYGQMFEACP+EFPQLRRTDVAT+++DIKQGGMMVPHF
Sbjct: 601 RRGSRGVRAPIKLESQTPVYNNQYGQMFEACPDEFPQLRRTDVATSVVDIKQGGMMVPHF 660

Query: 661 NSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEEREREERSGRIERVAG 720
           NSRATWVVFVSEG GSFEM CPHIQ GQWQRGRREEERHWRREEE EREERSGR ERVAG
Sbjct: 661 NSRATWVVFVSEGAGSFEMACPHIQSGQWQRGRREEERHWRREEEEEREERSGRFERVAG 720

Query: 721 RLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDREAKEL 780
           RLS+GGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRE+IMNE+DREAKEL
Sbjct: 721 RLSEGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRENIMNELDREAKEL 780

Query: 781 AFNVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIPLLSILKVA 788
           AFNVEGKQA+EIF+SQ+ESFFTEGPEGG RRSTER PLLSILK+A
Sbjct: 781 AFNVEGKQADEIFRSQRESFFTEGPEGGRRRSTERSPLLSILKLA 805

BLAST of HG10012704 vs. ExPASy Swiss-Prot
Match: Q9SEW4 (Vicilin Jug r 2.0101 (Fragment) OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 5.1e-113
Identity = 301/629 (47.85%), Postives = 407/629 (64.71%), Query Frame = 0

Query: 189 RGEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDENQRDPDWRRE 248
           RG  +D++EN RDP   REQ R+ QE  RR+ + +R+++Q +                R 
Sbjct: 1   RGRDDDDEENPRDP---REQYRQCQEYCRRQGQGQRQQQQCQ---------------IRC 60

Query: 249 QERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERREQERRRREQ------ 308
           +ER E+++R +E      ER+RR  RD +D+N RDP+ R EQ +++ ER+RR Q      
Sbjct: 61  EERLEEDQRSQE------ERERRRGRDVDDQNPRDPEQRYEQCQQQCERQRRGQEQTLCR 120

Query: 309 ---EQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRE---------RQPGG 368
              EQR ++ ERE  R RD     Q     QR  + + +  ERQR+         ++  G
Sbjct: 121 RRCEQRRQQEERERQRGRDRQDPQQQYHRCQRRCQIQEQSPERQRQCQQRCERQYKEQQG 180

Query: 369 RSRINQFEPR-----RTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGI 428
           R R  +  PR     R E++Q HNPYYF  Q  +SR  S+EG  + LERF+ERTELL+GI
Sbjct: 181 RERGPEASPRRESRGREEEQQRHNPYYFHSQSIRSRHESEEGEVKYLERFTERTELLRGI 240

Query: 429 KNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVIT 488
           +N R+ +L+A P T ++PHH DAESV  V RGRAT+T + QE R    ES+N+E GDVI 
Sbjct: 241 ENYRVVILDANPNTSMLPHHKDAESVAVVTRGRATLTLVSQETR----ESFNLECGDVIR 300

Query: 489 IPAGTTVYLANQE-NEDLQIVKLIQPVNNPGEFKDYLSGGGEA--QAYYSVFSNDVLEAA 548
           +PAG TVY+ NQ+ NE L++VKL+QPVNNPG+F++Y + G ++  Q+Y  VFSND+L AA
Sbjct: 301 VPAGATVYVINQDSNERLEMVKLLQPVNNPGQFREYYAAGAKSPDQSYLRVFSNDILVAA 360

Query: 549 LNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQRATSIRK---GGKGARALIKLESQT 608
           LN PRDRLER F Q+ +R G IIRASQE+LRALSQ A S  +   G + +   I L+S++
Sbjct: 361 LNTPRDRLERFFDQQEQREGVIIRASQEKLRALSQHAMSAGQRPWGRRSSGGPISLKSES 420

Query: 609 PVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSF 668
           P YSNQ+GQ FEACPEE  QL+  DV     +IK+G MMVPH+NS+AT VV+V EGTG +
Sbjct: 421 PSYSNQFGQFFEACPEEHRQLQEMDVLVNYAEIKRGAMMVPHYNSKATVVVYVVEGTGRY 480

Query: 669 EMGCPHIQGGQWQ-RGRREEERHWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPI 728
           EM CPH+    ++ +GRRE+          E EE +GR ++V  RL++G + VIPAGHPI
Sbjct: 481 EMACPHVSSQSYEGQGRREQ----------EEEESTGRFQKVTARLARGDIFVIPAGHPI 540

Query: 729 AIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQ 788
           AI AS NENLRL+GF IN ENNQR+FLAG+ +I+N+++REAKEL+FN+  ++ EEIF+SQ
Sbjct: 541 AITASQNENLRLLGFDINGENNQRDFLAGQNNIINQLEREAKELSFNMPREEIEEIFESQ 591

BLAST of HG10012704 vs. ExPASy Swiss-Prot
Match: B3STU4 (Vicilin Car i 2.0101 OS=Carya illinoinensis OX=32201 GN=pec2a1a PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.7e-108
Identity = 366/839 (43.62%), Postives = 503/839 (59.95%), Query Frame = 0

Query: 3   LSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAACA 62
           ++K K  L L    +FLA V   L +E E L +E+   +            +  EF  C 
Sbjct: 2   VTKAKIPLFLFLSALFLALVCSSLALETEDLSNELNPHHD--------PESHRWEFQQCQ 61

Query: 63  KRCGVNQKVSEICRQWCQVAEKHGAEQQRRCRQACEERLGEQERGEDVEE-YKRRDPERE 122
           +RC                 E+ G  Q ++C++ CEE+L E+ER  + EE    R+P ++
Sbjct: 62  ERC---------------QHEERGQRQAQQCQRRCEEQLREREREREREEIVDPREPRKQ 121

Query: 123 REEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRREQ 182
            E+ R    +++ R++ + ER  ER      + +   ERER ERRR     + E  R  +
Sbjct: 122 YEQCRETCEKQDPRQQPQCERRCER------QFQEQQERERRERRRGRDDDDKENPRDPR 181

Query: 183 ER-RERERRGEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDENQ 242
           E+ R+ E    R+ + + Q+     R +ER E+E+RR    QE RER RRG   D++EN 
Sbjct: 182 EQYRQCEEHCRRQGQGQRQQQQCQSRCEERFEEEQRR----QEERER-RRGRDNDDEENP 241

Query: 243 RDPDWRREQERREQERRRR--------------------EQEQERRERQRRGERDDEDEN 302
           RDP   REQ R+ QE  RR                    E+++++ ER+RR  RD++D+N
Sbjct: 242 RDP---REQYRQCQEHCRRQGQGQRQQQQCQSRCEERLEEEQRKQEERERRRGRDEDDQN 301

Query: 303 QRDPDWRKEQ-------ERREQE----RRRREQEQREREWEREHGRRRDENQRGQGGREE 362
            RDP+ R EQ       +RR QE    RRR EQ++++ E ER+ GR R + Q+ Q  R +
Sbjct: 302 PRDPEQRYEQCQQQCERQRRGQEQQLCRRRCEQQRQQEERERQRGRDRQDPQQ-QYHRCQ 361

Query: 363 QRSKEEERKRE---------ERQRERQPGGRSRINQFEPR-----RTEQEQSHNPYYFQE 422
           +R + +E+  E         ERQ + Q G     +Q  PR     R E++Q HNPYYF  
Sbjct: 362 RRCQTQEQSPERQRQCQQRCERQYKEQQGREWGPDQASPRRESRGREEEQQRHNPYYFHS 421

Query: 423 QRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVV 482
           Q  +SR  S EG  + LERF+ERTELL+GI+N R+ +LEA P TF++P+H DAESV+ V 
Sbjct: 422 QGLRSRHESGEGEVKYLERFTERTELLRGIENYRVVILEANPNTFVLPYHKDAESVIVVT 481

Query: 483 RGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQE-NEDLQIVKLIQPVNNP 542
           RGRAT+T + QE    R+ES+N+E GDVI +PAG T Y+ NQ+ NE L++VKL+QPVNNP
Sbjct: 482 RGRATLTFVSQE----RRESFNLEYGDVIRVPAGATEYVINQDSNERLEMVKLLQPVNNP 541

Query: 543 GEFKDYLSGGGEA-QAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLR 602
           G+F++Y + G ++ ++Y  VFSND+L AALN PRDRLER F Q+ +R G IIRASQE+LR
Sbjct: 542 GQFREYYAAGAQSTESYLRVFSNDILVAALNTPRDRLERFFDQQEQREGVIIRASQEKLR 601

Query: 603 ALSQRATSIRK---GGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALL 662
           ALSQ A S  +   G + +   I L+SQ   YSNQ+GQ FEACPEE  QL+  DV     
Sbjct: 602 ALSQHAMSAGQRPWGRRSSGGPISLKSQRSSYSNQFGQFFEACPEEHRQLQEMDVLVNYA 661

Query: 663 DIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQ--WQRGRREEERHWRREEER 722
           +IK+G MMVPH+NS+AT VV+V EGTG FEM CPH    Q    +GRRE+E         
Sbjct: 662 EIKRGAMMVPHYNSKATVVVYVVEGTGRFEMACPHDVSSQSYEYKGRREQE--------- 721

Query: 723 EREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGR 782
           E E  +G+ ++V  RL++G + VIPAGHPIAI AS NENLRLVGFGIN +NNQRNFLAG+
Sbjct: 722 EEESSTGQFQKVTARLARGDIFVIPAGHPIAITASQNENLRLVGFGINGKNNQRNFLAGQ 781

Query: 783 ESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIPLLSILKVA 788
            +I+N+++REAKEL+FN+  ++ EEIF+ Q ES+F        R      PL SIL  A
Sbjct: 782 NNIINQLEREAKELSFNMPREEIEEIFERQVESYFVPMERQSRRGQGRDHPLASILDFA 789

BLAST of HG10012704 vs. ExPASy Swiss-Prot
Match: P09799 (Vicilin GC72-A OS=Gossypium hirsutum OX=3635 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.5e-75
Identity = 249/618 (40.29%), Postives = 364/618 (58.90%), Query Frame = 0

Query: 190 GEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDENQRDPDWRREQ 249
           G R ++D  QR  D R    +R Q   R + EQ++ E   R E + ++E QRD +   + 
Sbjct: 30  GRRSEDDPQQRYEDCR----KRCQLETRGQTEQDKCE--DRSETQLKEEQQRDGE---DP 89

Query: 250 ERREQERRRREQEQERR-----ERQRRGERDDEDENQRDPDWRKEQERREQERRRREQEQ 309
           +RR Q+ R+  Q++ERR     E+  R + + + + Q D  +++ Q+R + + +R E++Q
Sbjct: 90  QRRYQDCRQHCQQEERRLRPHCEQSCREQYEKQQQQQPDKQFKECQQRCQWQEQRPERKQ 149

Query: 310 REREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQ 369
           +  +  RE           Q   +  + + E + REE + E   G             EQ
Sbjct: 150 QCVKECRE-----------QYQEDPWKGERENKWREEEEEESDEG-------------EQ 209

Query: 370 EQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPH 429
           +Q +NPYYF  + FQ RFR + G++RVL+RF+++  LL+GI   R+A+LEA P TF++PH
Sbjct: 210 QQRNNPYYFHRRSFQERFREEHGNFRVLQRFADKHHLLRGINEFRIAILEANPNTFVLPH 269

Query: 430 HLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQEN-EDLQ 489
           H DAE +  V  GR T+T +  E     KESYNV  G V+ IPAG+TVYLANQ+N E L 
Sbjct: 270 HCDAEKIYVVTNGRGTVTFVTHE----NKESYNVVPGVVVRIPAGSTVYLANQDNREKLT 329

Query: 490 IVKLIQPVNNPGEFKDYLSGGGE-AQAYYSVFSNDVLEAALNIPRDRLE-----RIFKQK 549
           I  L +PVNNPG+F+ +   G E  Q+Y  +FS ++LEA  N   ++L+     R   ++
Sbjct: 330 IAVLHRPVNNPGQFQKFFPAGQENPQSYLRIFSREILEAVFNTRSEQLDELPGGRQSHRR 389

Query: 550 SERRGKIIRASQEQLRALSQRATSIRKGGKGARA-LIKLESQTPVYSNQYGQMFEACPEE 609
            + +G   +ASQEQ+RALSQ ATS R  GKG+      L SQTP YSNQ G+ +EACP  
Sbjct: 390 QQGQGMFRKASQEQIRALSQGATSPR--GKGSEGYAFNLLSQTPRYSNQNGRFYEACPRN 449

Query: 610 F-PQLRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHI--QGGQWQR 669
           F  QLR  D +    +I +G + VPH+NS+AT+VV V+EG G  EM CPH+  Q   W  
Sbjct: 450 FQQQLREVDSSVVAFEINKGSIFVPHYNSKATFVVLVTEGNGHVEMVCPHLSRQSSDWS- 509

Query: 670 GRREEERHWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGF 729
            R EEE     +EE+E E RSG+ +RV  +LS G + V+PAGHP+  +AS NE+L L+GF
Sbjct: 510 SREEEE-----QEEQEVERRSGQYKRVRAQLSTGNLFVVPAGHPVTFVASQNEDLGLLGF 569

Query: 730 GI-NAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQ-KESFFTEGPE--G 788
           G+ N ++N+R F+AG+ + + + DR+AKELAF VE +  +E+F +  +ES+F  G +  G
Sbjct: 570 GLYNGQDNKRIFVAGKTNNVRQWDRQAKELAFGVESRLVDEVFNNNPQESYFVSGRDRRG 602

BLAST of HG10012704 vs. ExPASy Swiss-Prot
Match: Q9SPL3 (Vicilin-like antimicrobial peptides 2-3 (Fragment) OS=Macadamia integrifolia OX=60698 GN=AMP2-3 PE=1 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 3.1e-73
Identity = 256/649 (39.45%), Postives = 373/649 (57.47%), Query Frame = 0

Query: 153 ENERDPEREREERRREEQRREHEQRRREQERRERERRGEREDEDENQRDPDWRREQ---- 212
           +N+ DP+ E ++ +R  +++E + R+++  +R  +   E E+E   QRDP  + EQ    
Sbjct: 32  DNQEDPQTECQQCQRRCRQQESDPRQQQYCQRRCKEICEEEEEYNRQRDPQQQYEQCQKR 91

Query: 213 -ERREQERRRREQEQERRERQRRGEREDEDENQRDPDWRREQERREQERRRREQEQERRE 272
            +RRE E R  +  Q+R ER                  R E+E+R+Q++R  EQ++E  E
Sbjct: 92  CQRRETEPRHMQICQQRCER------------------RYEKEKRKQQKRYEEQQREDEE 151

Query: 273 RQRRGERDDEDENQRDPDWRKEQERREQERRRREQEQREREWEREHGRRRDENQRGQGGR 332
           +    ER  E +N+RDP      ++RE E  RR  EQ+E   + +  RR  E QR  G  
Sbjct: 152 KYE--ERMKEGDNKRDP------QQREYEDCRRHCEQQEPRLQYQCQRRCQEQQRQHG-- 211

Query: 333 EEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRSDEG 392
                      R       Q GG  R  +      E++QS NPYYF E+   +RFR++EG
Sbjct: 212 -----------RGGDLMNPQRGGSGRYEE-----GEEKQSDNPYYFDERSLSTRFRTEEG 271

Query: 393 HWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQE 452
           H  VLE F  R++LL+ +KN RL +LEA P  F++P HLDA+++L V+ GR  +  I ++
Sbjct: 272 HISVLENFYGRSKLLRALKNYRLVLLEANPNAFVLPTHLDADAILLVIGGRGALKMIHRD 331

Query: 453 KRETRKESYNVERGDVITIPAGTTVYLANQE-NEDLQIVKLIQPVNNPGEFKDYLSGGGE 512
            R    ESYN+E GDVI IPAGTT YL N++ NE L I K +Q ++ PG++K++   GG+
Sbjct: 332 NR----ESYNLECGDVIRIPAGTTFYLINRDNNERLHIAKFLQTISTPGQYKEFFPAGGQ 391

Query: 513 -AQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQ-----RAT 572
             + Y S FS ++LEAALN   +RL  +  Q  +R G IIRASQEQ+R L++     R  
Sbjct: 392 NPEPYLSTFSKEILEAALNTQTERLRGVLGQ--QREGVIIRASQEQIRELTRDDSESRRW 451

Query: 573 SIRKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVP 632
            IR+GG+ +R    L ++ P+YSN+YGQ +E  PE++ QL+  DV+  + +I QG MM P
Sbjct: 452 HIRRGGESSRGPYNLFNKRPLYSNKYGQAYEVKPEDYRQLQDMDVSVFIANITQGSMMGP 511

Query: 633 HFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEEREREERSGRIERV 692
            FN+R+T VV V+ G    EM CPH+ G    RG            +R  EE     E+V
Sbjct: 512 FFNTRSTKVVVVASGEADVEMACPHLSGRHGGRG----------GGKRHEEEEEVHYEQV 571

Query: 693 AGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRE-SIMNEVDREA 752
             RLS+   +V+ AGHP+  ++S NENL L  FGINA+NN  NFLAGRE +++ +++ +A
Sbjct: 572 RARLSKREAIVVLAGHPVVFVSSGNENLLLFAFGINAQNNHENFLAGRERNVLQQIEPQA 620

Query: 753 KELAFNVEGKQAEEIFKSQKESFFTEGP----EGGHRRSTERIPLLSIL 785
            ELAF    K+ EE+F SQ ES F  GP    +   R + ++ PL+SIL
Sbjct: 632 MELAFAASRKEVEELFNSQDESIFFPGPRQHQQQSPRSTKQQQPLVSIL 620

BLAST of HG10012704 vs. ExPASy Swiss-Prot
Match: P09801 (Vicilin C72 OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.7e-71
Identity = 232/589 (39.39%), Postives = 353/589 (59.93%), Query Frame = 0

Query: 190 GEREDEDENQRDPDWRREQERREQERRRREQEQERRE--RQRRGEREDEDENQRDPDWRR 249
           G R D+D  +R  D RR   R E + R ++++Q+  E  + + GE+ D+ +  R  D +R
Sbjct: 30  GRRGDDDPPKRYEDCRR---RCEWDTRGQKEQQQCEESCKSQYGEK-DQQQRHRPEDPQR 89

Query: 250 EQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERREQERRRREQEQRER 309
             E  +QE R++E+ Q  + +QR  +R ++++ Q    +++ Q+   Q+ +R E   R++
Sbjct: 90  RYEECQQECRQQEERQRPQCQQRCLKRFEQEQQQSQRQFQECQQHCHQQEQRPE---RKQ 149

Query: 310 EWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQS 369
           +  RE   R  EN         +R +EEE + EE +                   EQEQS
Sbjct: 150 QCVRECRERYQEN-------PWRREREEEAEEEETE-----------------EGEQEQS 209

Query: 370 HNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLD 429
           HNP++F  + FQSRFR + G++RVL+RF+ R  +L+GI   RL++LEA P TF++PHH D
Sbjct: 210 HNPFHFHRRSFQSRFREEHGNFRVLQRFASRHPILRGINEFRLSILEANPNTFVLPHHCD 269

Query: 430 AESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQEN-EDLQIVK 489
           AE +  V  GR T+T +  E     KESYNV  G V+ +PAG+TVYLANQ+N E L I  
Sbjct: 270 AEKIYLVTNGRGTLTFLTHE----NKESYNVVPGVVVRVPAGSTVYLANQDNKEKLIIAV 329

Query: 490 LIQPVNNPGEFKDYLSGGGE-AQAYYSVFSNDVLEAALNIPRDRLERIF-----KQKSER 549
           L +PVNNP +F+++   G +  Q+Y   FS ++LE A N   ++L+ +F      ++ + 
Sbjct: 330 LHRPVNNPRQFEEFFPAGSQRPQSYLRAFSREILEPAFNTRSEQLDELFGGRQSHRRQQG 389

Query: 550 RGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQL 609
           +G   +ASQEQ+RALSQ ATS R+   G R    L  +TP YSNQ G+ +EACP EF QL
Sbjct: 390 QGMFRKASQEQIRALSQEATSPRE-KSGERFAFNLLYRTPRYSNQNGRFYEACPREFRQL 449

Query: 610 RRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEER 669
              +V  + L + QG + VPH+NS+AT+VV V+EG G  EM  PH+     ++   EEE 
Sbjct: 450 SDINVTVSALQLNQGSIFVPHYNSKATFVVLVNEGNGYVEMVSPHLP----RQSSFEEEE 509

Query: 670 HWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFG-----I 729
             ++E+E+E E RSG+  ++  +LS+G + V+PA  P+  +AS N+NLR+ GFG     I
Sbjct: 510 EQQQEQEQEEERRSGQYRKIRSQLSRGDIFVVPANFPVTFVASQNQNLRMTGFGLYNQNI 569

Query: 730 NAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQ-KESFF 764
           N ++NQR F+AG+ + + + D +AKELAF V  +  +EIF +  +ES+F
Sbjct: 570 NPDHNQRIFVAGKINHVRQWDSQAKELAFGVSSRLVDEIFNNNPQESYF 578

BLAST of HG10012704 vs. ExPASy TrEMBL
Match: A0A5D3CZ82 (Vicilin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00600 PE=4 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 679/791 (85.84%), Postives = 742/791 (93.81%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAA 60
           MA SKVKFRLCLLA T+FLACVSVGLG EGESLGS VG DNGCVNGC+ELKGKN+DE+AA
Sbjct: 1   MAFSKVKFRLCLLALTLFLACVSVGLGAEGESLGSGVGVDNGCVNGCQELKGKNLDEYAA 60

Query: 61  CAKRCGVNQKVSEICRQWCQVAEKHGAE-QQRRCRQACEERLGEQERGEDVEEYKRRDPE 120
           C KRCGVNQ+V+EICRQWCQV E HG E QQRRCRQ CEERL +Q++GEDVE+ K RDPE
Sbjct: 61  CEKRCGVNQEVTEICRQWCQVMEPHGGEQQQRRCRQECEERLRDQKQGEDVED-KWRDPE 120

Query: 121 REREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRR 180
           REREEQRRREHEREERRRRE ERE ERGRG RDEN+RDPEREREERRREEQRRE EQRRR
Sbjct: 121 REREEQRRREHEREERRRRERERERERGRGHRDENDRDPEREREERRREEQRREQEQRRR 180

Query: 181 EQERRERERRGEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDEN 240
           EQERRERERRGEREDE+ENQR PDWRREQERREQERRRREQE+ERRER+RRGEREDE+EN
Sbjct: 181 EQERRERERRGEREDEEENQRGPDWRREQERREQERRRREQERERRERERRGEREDEEEN 240

Query: 241 QRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERREQERRR 300
           QRDPD RREQERREQERRRREQEQERRER+RRGER+DE+ENQRDPD R+EQERREQERRR
Sbjct: 241 QRDPDRRREQERREQERRRREQEQERRERERRGEREDEEENQRDPDRRREQERREQERRR 300

Query: 301 REQEQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGRSRINQFEP 360
           REQEQREREWEREHGR   + +R +GG+EE+ S++EE +REER+RER+ GGRSR N+   
Sbjct: 301 REQEQREREWEREHGRGGSQERR-RGGQEEEISRKEEWEREERRREREHGGRSRANEVNS 360

Query: 361 RRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQT 420
           R TEQEQSHNPYYFQ+++FQSRFRSD+G WRVLERFSER+ELL+G+KNQRLA+LEARPQT
Sbjct: 361 RWTEQEQSHNPYYFQDRQFQSRFRSDQGQWRVLERFSERSELLRGLKNQRLAILEARPQT 420

Query: 421 FIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQEN 480
           FIIPHH+DAESVLFVV+GRATITTIVQE++ETRKESYNVERGDV+TIPAGTTVYLANQEN
Sbjct: 421 FIIPHHIDAESVLFVVKGRATITTIVQERKETRKESYNVERGDVVTIPAGTTVYLANQEN 480

Query: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSE 540
           EDLQIVKLIQPVNNPGEFKDYLSGGGEAQ+YYSVFS+DVLEA LNIPRDRLERIFKQ+ E
Sbjct: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQSYYSVFSDDVLEATLNIPRDRLERIFKQRGE 540

Query: 541 RRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQ 600
           RRGKIIRASQEQL+ALSQRATS++KGG+GAR+LIKLESQ+PVY+NQYGQM+EACP+EFPQ
Sbjct: 541 RRGKIIRASQEQLKALSQRATSVKKGGRGARSLIKLESQSPVYNNQYGQMYEACPDEFPQ 600

Query: 601 LRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEE 660
           LRRTDVAT++LDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPH+QG QWQRGRREEE
Sbjct: 601 LRRTDVATSVLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHVQGSQWQRGRREEE 660

Query: 661 RHWRREEERE-REERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720
           R WRREEERE  +ERS RIER+AGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE
Sbjct: 661 RQWRREEERELSDERSDRIERIAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720

Query: 721 NNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRS--TE 780
           NN+RNFLAGRE+IMNEVDREAKEL FNVEGKQAEE FKSQKESFFTEGPEGG RRS  TE
Sbjct: 721 NNKRNFLAGRENIMNEVDREAKELTFNVEGKQAEETFKSQKESFFTEGPEGGRRRSTETE 780

Query: 781 RIPLLSILKVA 788
           R PL SILK+A
Sbjct: 781 RTPLFSILKLA 789

BLAST of HG10012704 vs. ExPASy TrEMBL
Match: A0A6J1ITC1 (vicilin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479171 PE=4 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 663/796 (83.29%), Postives = 715/796 (89.82%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGAD-NGCVNGCEELKGKNVDEFA 60
           MALSKVK RLCLLAFT+FLAC+SVGLG +GESL S  G D +GCVN CEELKGKNVDEFA
Sbjct: 1   MALSKVKLRLCLLAFTLFLACLSVGLGDKGESLSSGAGVDHDGCVNRCEELKGKNVDEFA 60

Query: 61  ACAKRCGVNQKVS-----EICRQWCQVAEKHGAEQQRRCRQACEERLGEQE--RGEDVEE 120
           AC K CGVNQ+ S     E+CR  CQVAE+ G EQQR+C Q CEERL E+E  RGEDV+E
Sbjct: 61  ACKKGCGVNQRGSPRAEYEVCRLRCQVAER-GVEQQRKCEQVCEERLREREQGRGEDVDE 120

Query: 121 YKRRDPEREREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRR 180
            +RRDPE EREEQRRREHEREERRRRE ERE ERGRGRRDENERDP+RE+EER    QRR
Sbjct: 121 VERRDPEWEREEQRRREHEREERRRRERERERERGRGRRDENERDPKREQEER----QRR 180

Query: 181 EHEQRRREQERRERERRGER-EDEDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240
           E E+RRREQE+RERERRGER E++DENQRDPDWRREQERREQERRRREQEQERRERQRRG
Sbjct: 181 EQERRRREQEQRERERRGERDEEDDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240

Query: 241 EREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE 300
            R+DEDENQRDPDWRREQERREQERRRRE+EQERRER+ RG RD EDENQRDPDW     
Sbjct: 241 GRDDEDENQRDPDWRREQERREQERRRREREQERREREHRGGRDVEDENQRDPDW----- 300

Query: 301 RREQERRRREQEQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGR 360
           RREQERRRRE+EQREREWEREHGRR         GREEQRS+E+ER+R ERQ     GGR
Sbjct: 301 RREQERRRREEEQREREWEREHGRR---------GREEQRSREDERRRHERQH----GGR 360

Query: 361 SRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLA 420
           SR+NQ   RRTEQEQS+NPYYFQEQRFQSR+RSDEGHWRVLERFSER+ELLKGIKNQRLA
Sbjct: 361 SRVNQVAIRRTEQEQSNNPYYFQEQRFQSRYRSDEGHWRVLERFSERSELLKGIKNQRLA 420

Query: 421 VLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTT 480
           +LEARP TFI+PHHLDAE VL VVRGRATITT+VQEKRETRKESYNVE GDV+TIPAGTT
Sbjct: 421 LLEARPHTFIVPHHLDAECVLLVVRGRATITTVVQEKRETRKESYNVESGDVMTIPAGTT 480

Query: 481 VYLANQENEDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLE 540
           +YLANQENEDLQIVKL+QPVNNPGEFKDYLS GGE+QAYYSVFSNDVLEAALNIPRD+LE
Sbjct: 481 LYLANQENEDLQIVKLVQPVNNPGEFKDYLSAGGESQAYYSVFSNDVLEAALNIPRDKLE 540

Query: 541 RIFKQKSERRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFE 600
           RIFKQ+ ER GKI+RASQEQLRALSQRATS+RKG +G RA IKLESQTPVY+NQYGQMFE
Sbjct: 541 RIFKQRRERGGKIVRASQEQLRALSQRATSVRKGSRGVRAPIKLESQTPVYNNQYGQMFE 600

Query: 601 ACPEEFPQLRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQW 660
           ACP+EFPQLRRTDVAT+++DIKQGGMMVPHFNSRATWVVFVSEG GSFEM CPHIQ  QW
Sbjct: 601 ACPDEFPQLRRTDVATSVVDIKQGGMMVPHFNSRATWVVFVSEGAGSFEMACPHIQSSQW 660

Query: 661 QRGRREEERHWRREEEREREERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLV 720
           QRGRREEERHWRREEE EREERSGR ERVAGRLS+GGVLVIPAGHPIAIMASPNENLRLV
Sbjct: 661 QRGRREEERHWRREEEEEREERSGRFERVAGRLSEGGVLVIPAGHPIAIMASPNENLRLV 720

Query: 721 GFGINAENNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGH 780
           GFGINAENN RNFLAGRE+IMNE+DREAKELAFNVEGKQA+EIF+SQ+ESFFTEGPEGG 
Sbjct: 721 GFGINAENNHRNFLAGRENIMNELDREAKELAFNVEGKQADEIFRSQRESFFTEGPEGGR 773

Query: 781 RRSTERIPLLSILKVA 788
           RRSTER PLLSILK+A
Sbjct: 781 RRSTERSPLLSILKLA 773

BLAST of HG10012704 vs. ExPASy TrEMBL
Match: A0A6J1GW03 (vicilin-like OS=Cucurbita moschata OX=3662 GN=LOC111457935 PE=4 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 669/825 (81.09%), Postives = 722/825 (87.52%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGAD-NGCVNGCEELKGKNVDEFA 60
           MALSKVK RLCLLAFT+FLAC+SVGLG +GESL S  G D +GCVN CEELKGKNVDEFA
Sbjct: 1   MALSKVKLRLCLLAFTLFLACLSVGLGAKGESLSSGAGVDHDGCVNRCEELKGKNVDEFA 60

Query: 61  ACAKRCGVNQKVS-----EICRQWCQVAEKHGAEQQRRCRQACEERLGEQE--RGEDVEE 120
           AC K CGVNQ+ S     E+CR  CQVAE+ G EQQRRC Q CEERL E+E  RGEDV+E
Sbjct: 61  ACKKGCGVNQRGSPRAEYEVCRLRCQVAER-GVEQQRRCEQVCEERLREREQGRGEDVDE 120

Query: 121 YKRRDPEREREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRR 180
            +RRDPE EREEQRRREHEREERRRRE ERE ERGRGRRD NERDP+RE+EER    QRR
Sbjct: 121 VERRDPEWEREEQRRREHEREERRRRERERERERGRGRRDLNERDPKREQEER----QRR 180

Query: 181 EHEQRRREQERRERERRGER-EDEDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240
           E E+RRREQE+RERERRGER E++DENQRDPDWRREQERREQERRRREQEQERRERQRRG
Sbjct: 181 EQERRRREQEQRERERRGERDEEDDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240

Query: 241 EREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE 300
            R+DEDENQRDPDWRREQERREQERRRR  EQERRERQRRGERDDEDENQRDPDWR+EQE
Sbjct: 241 GRDDEDENQRDPDWRREQERREQERRRR--EQERRERQRRGERDDEDENQRDPDWRREQE 300

Query: 301 -----------------------------RREQERRRREQEQREREWEREHGRRRDENQR 360
                                        RREQERRRRE+EQREREWEREHGRR      
Sbjct: 301 RREQEQERRERERRGGRDDEDENQRDPDWRREQERRRREEEQREREWEREHGRR------ 360

Query: 361 GQGGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRF 420
              GREEQRS+E+ER+R+ERQ     GGRSR+NQ   RRTE EQS+NPYYFQEQRFQSR+
Sbjct: 361 ---GREEQRSREDERRRQERQH----GGRSRVNQVAIRRTELEQSNNPYYFQEQRFQSRY 420

Query: 421 RSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATIT 480
           RSD+GHWRVLE+FS+R+ELLKGIKNQRLA+LEARP TFI+PHHLDAE VL VVRGRATIT
Sbjct: 421 RSDQGHWRVLEKFSDRSELLKGIKNQRLAILEARPHTFIVPHHLDAECVLLVVRGRATIT 480

Query: 481 TIVQEKRETRKESYNVERGDVITIPAGTTVYLANQENEDLQIVKLIQPVNNPGEFKDYLS 540
           T+VQEKRETRKESYNVE GDV+TIPAGTT+YLANQENEDLQIVKL+QPVNNPGEFKDYLS
Sbjct: 481 TVVQEKRETRKESYNVESGDVMTIPAGTTLYLANQENEDLQIVKLVQPVNNPGEFKDYLS 540

Query: 541 GGGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQRATSI 600
            GGEAQAYYSVFSNDVLEAALNIPRD+LERIFKQ+ ER GKIIRASQEQLRALSQRATS+
Sbjct: 541 AGGEAQAYYSVFSNDVLEAALNIPRDKLERIFKQRRERGGKIIRASQEQLRALSQRATSV 600

Query: 601 RKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVPHF 660
           R+G +G RA IKLESQTPVY+NQYGQMFEACP+EFPQLRRTDVAT+++DIKQGGMMVPHF
Sbjct: 601 RRGSRGVRAPIKLESQTPVYNNQYGQMFEACPDEFPQLRRTDVATSVVDIKQGGMMVPHF 660

Query: 661 NSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEEREREERSGRIERVAG 720
           NSRATWVVFVSEG GSFEM CPHIQ GQWQRGRREEERHWRREEE EREERSGR ERVAG
Sbjct: 661 NSRATWVVFVSEGAGSFEMACPHIQSGQWQRGRREEERHWRREEEEEREERSGRFERVAG 720

Query: 721 RLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDREAKEL 780
           RLS+GGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRE+IMNE+DREAKEL
Sbjct: 721 RLSEGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRENIMNELDREAKEL 780

Query: 781 AFNVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIPLLSILKVA 788
           AFNVEGKQA+EIF+SQ+ESFFTEGPEGG RRSTER PLLSILK+A
Sbjct: 781 AFNVEGKQADEIFRSQRESFFTEGPEGGRRRSTERSPLLSILKLA 805

BLAST of HG10012704 vs. ExPASy TrEMBL
Match: A0A5A7U6W0 (Conglutin beta 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G005880 PE=4 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 643/791 (81.29%), Postives = 702/791 (88.75%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGADNGCVNGCEELKGKNVDEFAA 60
           MA SKVKFRLCLLA T+FLACVSVGLG EGESLGS VG DNGCVNGC+ELKGKN+DE+AA
Sbjct: 1   MAFSKVKFRLCLLALTLFLACVSVGLGAEGESLGSGVGVDNGCVNGCQELKGKNLDEYAA 60

Query: 61  CAKRCGVNQKVSEICRQWCQVAEKHGAE-QQRRCRQACEERLGEQERGEDVEEYKRRDPE 120
           C KRCGVNQ+V+EICRQWCQV E HG E QQRRCRQ CEERL +Q++GEDVE+ K RDPE
Sbjct: 61  CEKRCGVNQEVTEICRQWCQVMEPHGGEQQQRRCRQECEERLRDQKQGEDVED-KWRDPE 120

Query: 121 REREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRREHEQRRR 180
           REREEQRRREHEREERRRRE ERE ERGRG RDEN+RDPEREREERRREEQRRE EQRRR
Sbjct: 121 REREEQRRREHEREERRRRERERERERGRGHRDENDRDPEREREERRREEQRREQEQRRR 180

Query: 181 EQERRERERRGEREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGEREDEDEN 240
           EQERRERERRGEREDE+ENQR PDWRREQERREQERRRREQE+ERRER+RRGEREDE+EN
Sbjct: 181 EQERRERERRGEREDEEENQRGPDWRREQERREQERRRREQERERRERERRGEREDEEEN 240

Query: 241 QRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQERREQERRR 300
           QRDPD RR                                         EQERREQERRR
Sbjct: 241 QRDPDRRR-----------------------------------------EQERREQERRR 300

Query: 301 REQEQREREWEREHGRRRDENQRGQGGREEQRSKEEERKREERQRERQPGGRSRINQFEP 360
           REQEQREREWEREHGR   + +R +GG+EE+ S++EE +REER+RER+ GGRSR N+   
Sbjct: 301 REQEQREREWEREHGRGGSQERR-RGGQEEEISRKEEWEREERRREREHGGRSRANEVNS 360

Query: 361 RRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLERFSERTELLKGIKNQRLAVLEARPQT 420
           R TEQEQSHNPYYFQ+++FQSRFRSD+G WRVLERFSER+ELL+G+KNQRLA+LEARPQT
Sbjct: 361 RWTEQEQSHNPYYFQDRQFQSRFRSDQGQWRVLERFSERSELLRGLKNQRLAILEARPQT 420

Query: 421 FIIPHHLDAESVLFVVRGRATITTIVQEKRETRKESYNVERGDVITIPAGTTVYLANQEN 480
           FIIPHH+DAESVLFVV+GRATITTIVQE++ETRKESYNVERGDV+TIPAGTTVYLANQEN
Sbjct: 421 FIIPHHIDAESVLFVVKGRATITTIVQERKETRKESYNVERGDVVTIPAGTTVYLANQEN 480

Query: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSE 540
           EDLQIVKLIQPVNNPGEFKDYLSGGGEAQ+YYSVFS+DVLEA LNIPRDRLERIFKQ+ E
Sbjct: 481 EDLQIVKLIQPVNNPGEFKDYLSGGGEAQSYYSVFSDDVLEATLNIPRDRLERIFKQRGE 540

Query: 541 RRGKIIRASQEQLRALSQRATSIRKGGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQ 600
           RRGKIIRASQEQL+ALSQRATS++KGG+GAR+LIKLESQ+PVY+NQYGQM+EACP+EFPQ
Sbjct: 541 RRGKIIRASQEQLKALSQRATSVKKGGRGARSLIKLESQSPVYNNQYGQMYEACPDEFPQ 600

Query: 601 LRRTDVATALLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEE 660
           LRRTDVAT++LDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPH+QG QWQRGRREEE
Sbjct: 601 LRRTDVATSVLDIKQGGMMVPHFNSRATWVVFVSEGTGSFEMGCPHVQGSQWQRGRREEE 660

Query: 661 RHWRREEERE-REERSGRIERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720
           R WRREEERE  +ERS RIER+AGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE
Sbjct: 661 RQWRREEERELSDERSDRIERIAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAE 720

Query: 721 NNQRNFLAGRESIMNEVDREAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRS--TE 780
           NN+RNFLAGRE+IMNEVDREAKEL FNVEGKQAEE FKSQKESFFTEGPEGG RRS  TE
Sbjct: 721 NNKRNFLAGRENIMNEVDREAKELTFNVEGKQAEETFKSQKESFFTEGPEGGRRRSTETE 748

Query: 781 RIPLLSILKVA 788
           R PL SILK+A
Sbjct: 781 RTPLFSILKLA 748

BLAST of HG10012704 vs. ExPASy TrEMBL
Match: Q9ZWI3 (PV100 OS=Cucurbita maxima OX=3661 GN=LOC111479171 PE=1 SV=1)

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 662/823 (80.44%), Postives = 717/823 (87.12%), Query Frame = 0

Query: 1   MALSKVKFRLCLLAFTIFLACVSVGLGVEGESLGSEVGAD-NGCVNGCEELKGKNVDEFA 60
           MALSKVK RLCLLAFT+FLAC+SVGLG +GESL S  G D +GCVN CEELKGKNVDEFA
Sbjct: 1   MALSKVKLRLCLLAFTLFLACLSVGLGDKGESLSSGAGVDHDGCVNRCEELKGKNVDEFA 60

Query: 61  ACAKRCGVNQKVS-----EICRQWCQVAEKHGAEQQRRCRQACEERLGEQE--RGEDVEE 120
           AC K CGVNQ+ S     E+CR  CQVAE+ G EQQR+C Q CEERL E+E  RGEDV+E
Sbjct: 61  ACKKGCGVNQRGSPRAEYEVCRLRCQVAER-GVEQQRKCEQVCEERLREREQGRGEDVDE 120

Query: 121 YKRRDPEREREEQRRREHEREERRRREHEREHERGRGRRDENERDPEREREERRREEQRR 180
            +RRDPE EREEQRRREHEREERRRRE ERE ERGRGRRDENERDP+RE+EER    QRR
Sbjct: 121 VERRDPEWEREEQRRREHEREERRRRERERERERGRGRRDENERDPKREQEER----QRR 180

Query: 181 EHEQRRREQERRERERRGER-EDEDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240
           E E+RRREQE+RERERRGER E++DENQRDPDWRREQERREQERRRREQEQERRERQRRG
Sbjct: 181 EQERRRREQEQRERERRGERDEEDDENQRDPDWRREQERREQERRRREQEQERRERQRRG 240

Query: 241 EREDEDENQRDPDWRREQERREQERRRREQEQERRERQRRGERDDEDENQRDPDWRKEQE 300
            R+DEDENQRDPDWRREQ       +RREQEQERRER+RRG RDDEDENQRDPDWR+EQE
Sbjct: 241 GRDDEDENQRDPDWRREQ-------KRREQEQERRERERRGGRDDEDENQRDPDWRREQE 300

Query: 301 RREQERRRREQEQRERE------------------WERE-HGRRRDENQR--------GQ 360
           RREQERRRRE+EQ  RE                  W RE   RRR+E QR        G+
Sbjct: 301 RREQERRRREREQERREREHRGGRDVEDENQRDPDWRREQERRRREEEQREREWEREHGR 360

Query: 361 GGREEQRSKEEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRS 420
            GREEQRS+E+ER+R ERQ     GGRSR+NQ   RRTEQEQS+NPYYFQEQRFQSR+RS
Sbjct: 361 RGREEQRSREDERRRHERQH----GGRSRVNQVAIRRTEQEQSNNPYYFQEQRFQSRYRS 420

Query: 421 DEGHWRVLERFSERTELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATITTI 480
           DEGHWRVLERFSER+ELLKGIKNQRLA+LEARP TFI+PHHLDAE VL VVRGRATITT+
Sbjct: 421 DEGHWRVLERFSERSELLKGIKNQRLALLEARPHTFIVPHHLDAECVLLVVRGRATITTV 480

Query: 481 VQEKRETRKESYNVERGDVITIPAGTTVYLANQENEDLQIVKLIQPVNNPGEFKDYLSGG 540
           VQEKRETRKESYNVE GDV+TIPAGTT+YLANQENEDLQIVKL+QPVNNPGEFKDYLS G
Sbjct: 481 VQEKRETRKESYNVESGDVMTIPAGTTLYLANQENEDLQIVKLVQPVNNPGEFKDYLSAG 540

Query: 541 GEAQAYYSVFSNDVLEAALNIPRDRLERIFKQKSERRGKIIRASQEQLRALSQRATSIRK 600
           GE+QAYYSVFSNDVLEAALNIPRD+LERIFKQ+ ER GKI+RASQEQLRALSQRATS+RK
Sbjct: 541 GESQAYYSVFSNDVLEAALNIPRDKLERIFKQRRERGGKIVRASQEQLRALSQRATSVRK 600

Query: 601 GGKGARALIKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGMMVPHFNS 660
           G +G RA IKLESQTPVY+NQYGQMFEACP+EFPQLRRTDVAT+++DIKQGGMMVPHFNS
Sbjct: 601 GSRGVRAPIKLESQTPVYNNQYGQMFEACPDEFPQLRRTDVATSVVDIKQGGMMVPHFNS 660

Query: 661 RATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEEREREERSGRIERVAGRL 720
           RATWVVFVSEG GSFEM CPHIQ  QWQRGRREEERHWRREEE EREERSGR ERVAGRL
Sbjct: 661 RATWVVFVSEGAGSFEMACPHIQSSQWQRGRREEERHWRREEEEEREERSGRFERVAGRL 720

Query: 721 SQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDREAKELAF 780
           S+GGVLVIPAGHPIAIMASPNENLRLVGFGINAENN RNFLAGRE+IMNE+DREAKELAF
Sbjct: 721 SEGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNHRNFLAGRENIMNELDREAKELAF 780

Query: 781 NVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIPLLSILKVA 788
           NVEGKQA+EIF+SQ+ESFFTEGPEGG RRSTER PLLSILK+A
Sbjct: 781 NVEGKQADEIFRSQRESFFTEGPEGGRRRSTERSPLLSILKLA 807

BLAST of HG10012704 vs. TAIR 10
Match: AT3G22630.1 (20S proteasome beta subunit D1 )

HSP 1 Score: 357.8 bits (917), Expect = 2.8e-98
Identity = 175/198 (88.38%), Postives = 189/198 (95.45%), Query Frame = 0

Query: 814  MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 873
            ME VFGLVGNGF IVAADTSAVHSIL+HK+NEDKIMVLDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1    MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60

Query: 874  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 933
            QKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD E+G SLYYIDYI
Sbjct: 61   QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120

Query: 934  ATLHKVDKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 993
            ATLHKVDKGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121  ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIK 180

Query: 994  IVDKNGAREVAWRESIKD 1012
            IVDK+GAR+ AWR+S+KD
Sbjct: 181  IVDKDGARDYAWRQSVKD 198

BLAST of HG10012704 vs. TAIR 10
Match: AT4G14800.1 (20S proteasome beta subunit D2 )

HSP 1 Score: 349.4 bits (895), Expect = 1.0e-95
Identity = 172/198 (86.87%), Postives = 183/198 (92.42%), Query Frame = 0

Query: 814  MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 873
            ME VFGLVGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1    MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 874  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 933
            QKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNIL+AGYDKE G SLYYIDYI
Sbjct: 61   QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120

Query: 934  ATLHKVDKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 993
            ATLHKVDKGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLV+APPNFVIK
Sbjct: 121  ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIK 180

Query: 994  IVDKNGAREVAWRESIKD 1012
            IVDK+GARE  WR S  D
Sbjct: 181  IVDKDGAREYGWRISTAD 198

BLAST of HG10012704 vs. TAIR 10
Match: AT4G14800.2 (20S proteasome beta subunit D2 )

HSP 1 Score: 339.0 bits (868), Expect = 1.4e-92
Identity = 172/214 (80.37%), Postives = 183/214 (85.51%), Query Frame = 0

Query: 814  MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 873
            ME VFGLVGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1    MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 874  QKNVALYQFRNGIPLTTAAAANFTRGELATALRK----------------NPYSVNILLA 933
            QKNV+LYQFRNGIPL+TAAAANFTRGELATALRK                NPYSVNIL+A
Sbjct: 61   QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKEFCATFQGYSDFCVLMNPYSVNILMA 120

Query: 934  GYDKETGPSLYYIDYIATLHKVDKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCIL 993
            GYDKE G SLYYIDYIATLHKVDKGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCIL
Sbjct: 121  GYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCIL 180

Query: 994  EIRSRLVVAPPNFVIKIVDKNGAREVAWRESIKD 1012
            EIRSRLV+APPNFVIKIVDK+GARE  WR S  D
Sbjct: 181  EIRSRLVIAPPNFVIKIVDKDGAREYGWRISTAD 214

BLAST of HG10012704 vs. TAIR 10
Match: AT3G22640.1 (cupin family protein )

HSP 1 Score: 248.4 bits (633), Expect = 2.4e-65
Identity = 158/462 (34.20%), Postives = 266/462 (57.58%), Query Frame = 0

Query: 334 EEERKREERQRERQPGGRSRINQFEPRRTEQEQSHNPYYFQEQRFQSRFRSDEGHWRVLE 393
           E  ++ E+    R+PGG S          E+E +++PY+F+++ F   F+S EG  RVL 
Sbjct: 33  EPPQQGEQEGPRRRPGGGS------GEGWEEESTNHPYHFRKRSFSDWFQSKEGFVRVLP 92

Query: 394 RFSERT-ELLKGIKNQRLAVLEARPQTFIIPHHLDAESVLFVVRGRATITTIVQEKRETR 453
           +F++    L +GI+N R +++E  P TF +PHHLDA++V  V++G+  I  +     +  
Sbjct: 93  KFTKHAPALFRGIENYRFSLVEMEPTTFFVPHHLDADAVFIVLQGKGVIEFVT----DKT 152

Query: 454 KESYNVERGDVITIPAGTTVYLAN-QENEDLQIVKLIQPVNNPGEFKDYLSGGGE-AQAY 513
           KES+++ +GDV+ IP+G T ++ N  +   L++ ++  PVNNPG +KDY     +  Q+Y
Sbjct: 153 KESFHITKGDVVRIPSGVTNFITNTNQTVPLRLAQITVPVNNPGNYKDYFPAASQFQQSY 212

Query: 514 YSVFSNDVLEAALNIPRDRLERIFKQKSE-RRGKIIRASQEQLRALSQRATS-------- 573
           ++ F+ +VL  + N+P + L R+  +  E  +G I R S +Q++ L++ ATS        
Sbjct: 213 FNGFTKEVLSTSFNVPEELLGRLVTRSKEIGQGIIRRISPDQIKELAEHATSPSNKHKAK 272

Query: 574 -IRKGGKGARAL---IKLESQTPVYSNQYGQMFEACPEEFPQLRRTDVATALLDIKQGGM 633
             ++  K  R L     L +  P+YSN +G   EA P+ + QL+   +A A  ++ QG +
Sbjct: 273 KEKEEDKDLRTLWTPFNLFAIDPIYSNDFGHFHEAHPKNYNQLQDLHIAAAWANMTQGSL 332

Query: 634 MVPHFNSRATWVVFVSEGTGSFEMGCPHIQGGQWQRGRREEERHWRREEEREREERSGRI 693
            +PHFNS+ T+V FV  G   FEM  P+    ++QRG+++    W  + + E E+ S  +
Sbjct: 333 FLPHFNSKTTFVTFVENGCARFEMATPY----KFQRGQQQ----WPGQGQEEEEDMSENV 392

Query: 694 ERVAGRLSQGGVLVIPAGHPIAIMASPNENLRLVGFGINAENNQRNFLAGRESIMNEVDR 753
            +V  R+ +G V ++PAGHP  I+ S +++   VGFGI A N++R FLAG E++++ ++ 
Sbjct: 393 HKVVSRVCKGEVFIVPAGHPFTIL-SQDQDFIAVGFGIYATNSKRTFLAGEENLLSNLNP 452

Query: 754 EAKELAFNVEGKQAEEIFKSQKESFFTEGPEGGHRRSTERIP 780
            A  + F V  K AE++F SQ  S+F         RS ++IP
Sbjct: 453 AATRVTFGVGSKVAEKLFTSQNYSYFAP-----TSRSQQQIP 470