HG10012672 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012672
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionReceptor-like serine/threonine-protein kinase
LocationChr01: 23325050 .. 23327404 (+)
RNA-Seq ExpressionHG10012672
SyntenyHG10012672
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCATTGAACTTTTACATCTCTCTCTACCACTGTTTCTCTGTTTCTTTCACTGTTACGCCCAAACCCAGTACCCAAACAACACAATCAAATTGGGTTCATCAATCATTGCTGGATCCAATGAATTCTGGCAATCTTCCTCCGGCGATTTTGCTTTCGGGTTTCACCAAATCGTCGATGGCAGATACCTTGTCGGCATAGTGTTCGACAAAGTTCCCGAGAGAACTTTGGCATGGTCCGCCAACAGAGACGACCCAGCAAAGGCTAACTCCACCATAATTCTGAAACCCACCGGCCAATTTGTGCTAATTCATGCCAACAACACCCAAGTTTCAATCTACAATGGCACAACCACAAGCTCTGCTTTCATGTCCAACAATGGCAACTTTATGCTACTGAATTCCTCCTCTAATCCCATATGGCAGAGCTTCGATCATCCAACCGACACCCTTTTGCCCGGACAGGCCCTCCGCATCGGCCAACGCCTGTTCTCCAATGCTAACGGGACTGTTGATTATTCCACCGGCCGGTTCATGTTGGAAGTTCAAGGTTTCGACGGTAATGTCATAATGTCTGCTTTTCGGTTTGCTGATCCTGCTTACAAATTTACCGGAACCGTCGATAACAAAAACGCCACCATCGTCTTCAACCAGACCACAGCTTTCTTGTATGTTGTCAATGGCACGACCATTATCTATCCTATGACAAGCCAAGCCCAGTTGACGGTGCCAGTAAATGACTACTACCACAGAGCAACCGTCGATGATCAGGGGAACTTCCAGCAGCTGATTCGGATCAAGAACGGCAGTGGCGGCGAGTGGAGATCTGTGTGGAAGTTCGTTGAGAATCCTTGTATGGTGAGTAACATTTGTGGGGTGTTTGGATTTTGCACCACAATTGATAATCAAAAGGTGAATTGTGAGTGTTTAGAAGGGTATTCGCCAATTGATCCAAACATACCTTCCAAAGGATGCTATCCAAATAAGATTATGGACTTTTGTTCAGATTCAAACTTCAAAATGGTTAAGCTTGAAAATGTCGATTTCCCATTTCTTAAGGACAACTCTGATGCAACAATGGTCCGAGCTACAGATGCGAACGAGTGCGAGGAAGTTTTGAGGAACGAGTGTTTCCGTACAGCGGCTGTTTATTTTGATGGAGCTTGTTACAAGAAGAGGATGCCATTGTTAAACGCAAGAGGAAACATTCCAAATACGAATAATCATGTGGCATTCCTTAAAGTTCCCACAATCAATAATGTCTATAGCAAAAAATCCCCTTCTAAGGAAGCTCTGCTAGCGATATTTGTGGTCTGTTCAACATTTGCTGTGGTTTTTATAGCCATGGCCATTTATTATCATCCCATCACAAAGGGTTTCTTTCAAAGGAAGAAGCCTACAAAGCCAAAGCCATTGGATGTGAATTTGAAGGTATTTTCATTTAACGAATTGAAAGAAGCAACTAATGGATTCAAAAACCAACTTGGGAGAGGAGCTTTCGGCAGAGTCTATAATGGAGTTTTGATGTTGAACGATCAAGAAGTAGAGGTAGCCGTGAAGCAATTGGAGAAGATGATTGAACAAGGAGAGAAGGAGTTCATTACAGAAGTCCAAGTGATTGGATTGACTCACCATAAAAACTTAGTTCGTTTATTTTGTTTCTGCAATGAAGGTGATCATCGGTTGTTGGTTTATGAGCTCATGAAAAATGGTCCTTTGTCAAATGTCCTGTTTGGGGAGAAAGAAAATCAAAACCCCAAATGGGAAAGTAGATCAAAAATGGTGTTGGAAATTGCAAATGGGTTGTTATATTTACACGAAGAGTGCGAAACCCAGATCATCCATTGCGATATAAAGCCACAAAACATTCTCCTTGATGACAATTACACAGCAAAAATCTCAGATTTCGGCTTAGCGAAACTGATGAAGAAAAACCAGACACGCACAGCCACAATGATAAGAGGAACAATGGGATATATGGCGCCAGAATGGCTAAAGAATGCACCAATTACAACAAAAGTAGATGTTTATAGCTTTGGTGTGATGTTGTTAGAGATCATATTTTGTAGAAGGCATGTTGAAGAAGAGACAGAAGGAGATGATGCAATAATGCTTGTGGATTGGGTTATAAGTTGTGTGAGAGCAGAAAGATTGAGAGACATAATAAGCCATGATTCTGAAGCAATGAATGAATATGGAAGGTTTGAAAGGATAACAATGGTGGGTTTATGGTGCATTTGTCCAAATCCTGCACTTAGGCCATCCATGAAAGAAGTTCTGCTGATGCTTGAAGGAAGCATTGAAGTGAGAATCCCACCATTGGTTGAAACAGATGCTTATTTGCTTGGTTAA

mRNA sequence

ATGGCCGCCATTGAACTTTTACATCTCTCTCTACCACTGTTTCTCTGTTTCTTTCACTGTTACGCCCAAACCCAGTACCCAAACAACACAATCAAATTGGGTTCATCAATCATTGCTGGATCCAATGAATTCTGGCAATCTTCCTCCGGCGATTTTGCTTTCGGGTTTCACCAAATCGTCGATGGCAGATACCTTGTCGGCATAGTGTTCGACAAAGTTCCCGAGAGAACTTTGGCATGGTCCGCCAACAGAGACGACCCAGCAAAGGCTAACTCCACCATAATTCTGAAACCCACCGGCCAATTTGTGCTAATTCATGCCAACAACACCCAAGTTTCAATCTACAATGGCACAACCACAAGCTCTGCTTTCATGTCCAACAATGGCAACTTTATGCTACTGAATTCCTCCTCTAATCCCATATGGCAGAGCTTCGATCATCCAACCGACACCCTTTTGCCCGGACAGGCCCTCCGCATCGGCCAACGCCTGTTCTCCAATGCTAACGGGACTGTTGATTATTCCACCGGCCGGTTCATGTTGGAAGTTCAAGGTTTCGACGGTAATGTCATAATGTCTGCTTTTCGGTTTGCTGATCCTGCTTACAAATTTACCGGAACCGTCGATAACAAAAACGCCACCATCGTCTTCAACCAGACCACAGCTTTCTTGTATGTTGTCAATGGCACGACCATTATCTATCCTATGACAAGCCAAGCCCAGTTGACGGTGCCAGTAAATGACTACTACCACAGAGCAACCGTCGATGATCAGGGGAACTTCCAGCAGCTGATTCGGATCAAGAACGGCAGTGGCGGCGAGTGGAGATCTGTGTGGAAGTTCGTTGAGAATCCTTGTATGGTGAGTAACATTTGTGGGGTGTTTGGATTTTGCACCACAATTGATAATCAAAAGGTGAATTGTGAGTGTTTAGAAGGGTATTCGCCAATTGATCCAAACATACCTTCCAAAGGATGCTATCCAAATAAGATTATGGACTTTTGTTCAGATTCAAACTTCAAAATGGTTAAGCTTGAAAATGTCGATTTCCCATTTCTTAAGGACAACTCTGATGCAACAATGGTCCGAGCTACAGATGCGAACGAGTGCGAGGAAGTTTTGAGGAACGAGTGTTTCCGTACAGCGGCTGTTTATTTTGATGGAGCTTGTTACAAGAAGAGGATGCCATTGTTAAACGCAAGAGGAAACATTCCAAATACGAATAATCATGTGGCATTCCTTAAAGTTCCCACAATCAATAATGTCTATAGCAAAAAATCCCCTTCTAAGGAAGCTCTGCTAGCGATATTTGTGGTCTGTTCAACATTTGCTGTGGTTTTTATAGCCATGGCCATTTATTATCATCCCATCACAAAGGGTTTCTTTCAAAGGAAGAAGCCTACAAAGCCAAAGCCATTGGATGTGAATTTGAAGGTATTTTCATTTAACGAATTGAAAGAAGCAACTAATGGATTCAAAAACCAACTTGGGAGAGGAGCTTTCGGCAGAGTCTATAATGGAGTTTTGATGTTGAACGATCAAGAAGTAGAGGTAGCCGTGAAGCAATTGGAGAAGATGATTGAACAAGGAGAGAAGGAGTTCATTACAGAAGTCCAAGTGATTGGATTGACTCACCATAAAAACTTAGTTCGTTTATTTTGTTTCTGCAATGAAGAAAGATTGAGAGACATAATAAGCCATGATTCTGAAGCAATGAATGAATATGGAAGGTTTGAAAGGATAACAATGGTGGGTTTATGGTGCATTTGTCCAAATCCTGCACTTAGGCCATCCATGAAAGAAGTTCTGCTGATGCTTGAAGGAAGCATTGAAGTGAGAATCCCACCATTGGTTGAAACAGATGCTTATTTGCTTGGTTAA

Coding sequence (CDS)

ATGGCCGCCATTGAACTTTTACATCTCTCTCTACCACTGTTTCTCTGTTTCTTTCACTGTTACGCCCAAACCCAGTACCCAAACAACACAATCAAATTGGGTTCATCAATCATTGCTGGATCCAATGAATTCTGGCAATCTTCCTCCGGCGATTTTGCTTTCGGGTTTCACCAAATCGTCGATGGCAGATACCTTGTCGGCATAGTGTTCGACAAAGTTCCCGAGAGAACTTTGGCATGGTCCGCCAACAGAGACGACCCAGCAAAGGCTAACTCCACCATAATTCTGAAACCCACCGGCCAATTTGTGCTAATTCATGCCAACAACACCCAAGTTTCAATCTACAATGGCACAACCACAAGCTCTGCTTTCATGTCCAACAATGGCAACTTTATGCTACTGAATTCCTCCTCTAATCCCATATGGCAGAGCTTCGATCATCCAACCGACACCCTTTTGCCCGGACAGGCCCTCCGCATCGGCCAACGCCTGTTCTCCAATGCTAACGGGACTGTTGATTATTCCACCGGCCGGTTCATGTTGGAAGTTCAAGGTTTCGACGGTAATGTCATAATGTCTGCTTTTCGGTTTGCTGATCCTGCTTACAAATTTACCGGAACCGTCGATAACAAAAACGCCACCATCGTCTTCAACCAGACCACAGCTTTCTTGTATGTTGTCAATGGCACGACCATTATCTATCCTATGACAAGCCAAGCCCAGTTGACGGTGCCAGTAAATGACTACTACCACAGAGCAACCGTCGATGATCAGGGGAACTTCCAGCAGCTGATTCGGATCAAGAACGGCAGTGGCGGCGAGTGGAGATCTGTGTGGAAGTTCGTTGAGAATCCTTGTATGGTGAGTAACATTTGTGGGGTGTTTGGATTTTGCACCACAATTGATAATCAAAAGGTGAATTGTGAGTGTTTAGAAGGGTATTCGCCAATTGATCCAAACATACCTTCCAAAGGATGCTATCCAAATAAGATTATGGACTTTTGTTCAGATTCAAACTTCAAAATGGTTAAGCTTGAAAATGTCGATTTCCCATTTCTTAAGGACAACTCTGATGCAACAATGGTCCGAGCTACAGATGCGAACGAGTGCGAGGAAGTTTTGAGGAACGAGTGTTTCCGTACAGCGGCTGTTTATTTTGATGGAGCTTGTTACAAGAAGAGGATGCCATTGTTAAACGCAAGAGGAAACATTCCAAATACGAATAATCATGTGGCATTCCTTAAAGTTCCCACAATCAATAATGTCTATAGCAAAAAATCCCCTTCTAAGGAAGCTCTGCTAGCGATATTTGTGGTCTGTTCAACATTTGCTGTGGTTTTTATAGCCATGGCCATTTATTATCATCCCATCACAAAGGGTTTCTTTCAAAGGAAGAAGCCTACAAAGCCAAAGCCATTGGATGTGAATTTGAAGGTATTTTCATTTAACGAATTGAAAGAAGCAACTAATGGATTCAAAAACCAACTTGGGAGAGGAGCTTTCGGCAGAGTCTATAATGGAGTTTTGATGTTGAACGATCAAGAAGTAGAGGTAGCCGTGAAGCAATTGGAGAAGATGATTGAACAAGGAGAGAAGGAGTTCATTACAGAAGTCCAAGTGATTGGATTGACTCACCATAAAAACTTAGTTCGTTTATTTTGTTTCTGCAATGAAGAAAGATTGAGAGACATAATAAGCCATGATTCTGAAGCAATGAATGAATATGGAAGGTTTGAAAGGATAACAATGGTGGGTTTATGGTGCATTTGTCCAAATCCTGCACTTAGGCCATCCATGAAAGAAGTTCTGCTGATGCTTGAAGGAAGCATTGAAGTGAGAATCCCACCATTGGTTGAAACAGATGCTTATTTGCTTGGTTAA

Protein sequence

MAAIELLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVFDKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFCSDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYSKKSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNEERLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPPLVETDAYLLG
Homology
BLAST of HG10012672 vs. NCBI nr
Match: XP_023527957.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.7 bits (1950), Expect = 3.1e-214
Identity = 410/783 (52.36%), Postives = 497/783 (63.47%), Query Frame = 0

Query: 1   MAAIELLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIV 60
           MAAI++L+ SLPL LCF HCYAQ    N  + L SSI+AGSN+FW S SG+FAFGFH+I 
Sbjct: 1   MAAIKVLYFSLPLLLCFLHCYAQ----NGIVNLKSSIVAGSNDFWVSPSGEFAFGFHEIT 60

Query: 61  DGRYLVGIVFDKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTT 120
             RYLVGIVFDK+PERTL WSANRD+PA A STII   TG+F LIHAN+T+VSIY+GT T
Sbjct: 61  PDRYLVGIVFDKIPERTLVWSANRDNPANAKSTIIFGSTGEFALIHANDTKVSIYDGTDT 120

Query: 121 SSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFM 180
           +SAFMS++GNF LLNSSS+PIWQSFDHPTDTLLPGQ L  G +LFSN NG  DYS GRFM
Sbjct: 121 TSAFMSDDGNFQLLNSSSDPIWQSFDHPTDTLLPGQVLPKGHQLFSNVNGKTDYSKGRFM 180

Query: 181 LEVQGFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQA 240
           L+V   DGNV++++FR  DPAYK++GT  + +++IVFN+TTA LYV NGTTI Y MT++ 
Sbjct: 181 LDVAD-DGNVLLTSFRNGDPAYKYSGT--SGSSSIVFNRTTALLYVFNGTTITYTMTTEQ 240

Query: 241 QLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTT 300
               PV DYYHR  +DDQGNF+QL R K GS  EW + WK+VE PC+V+NICGVFGFCT+
Sbjct: 241 --PPPVEDYYHRVILDDQGNFRQLYRSKTGS--EWETAWKWVERPCLVNNICGVFGFCTS 300

Query: 301 IDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFCS----DSNFKMVKLENVDFPFLKDN 360
            DN+ VNC CLEGYS IDPN PSKGC P+ +MDFCS      +FK+V+LE+ DFP+LKD 
Sbjct: 301 PDNETVNCSCLEGYSSIDPNTPSKGCQPDLVMDFCSLDSTHESFKIVRLEDADFPYLKD- 360

Query: 361 SDATMVRATDANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKV 420
           SD +MV   D ++CEE +R +CF +AAVYF+  CYKKRMPLLNAR +I +TNN VAFLKV
Sbjct: 361 SDVSMVGPLDDSQCEEAVRKDCFCSAAVYFNNGCYKKRMPLLNARRSISDTNNLVAFLKV 420

Query: 421 PTINNVYSKKSPSKEALLAIFVVCSTFAVVFIAMAIYYHP-ITKGFFQRKKPTKPKPLDV 480
           P  N+   K   S EALLAIFVVCSTFA++F  +++YY P +T+G F+ KKP K   L+V
Sbjct: 421 PINND--GKTLISNEALLAIFVVCSTFALLFAVVSVYYQPFLTRGLFKEKKPAKLNQLEV 480

Query: 481 NLKVFSFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFI 540
           NLK FS NELKEATNGF+ QLG GAFG VY+GVL L DQEVEVAVK+L+K+ E GEKEF+
Sbjct: 481 NLKAFSLNELKEATNGFQKQLGSGAFGTVYHGVLRLRDQEVEVAVKKLKKLTEHGEKEFL 540

Query: 541 TEVQVIGLTHHKNLVRLFCFCNE------------------------------------- 600
           TEVQ+IGLTHH+NL++L  FCNE                                     
Sbjct: 541 TEVQIIGLTHHRNLLQLLGFCNEGDDRLLVYEFMKNDTLSSFLFGGKENEKPKWESRAKM 600

Query: 601 ------------------------------------------------------------ 621
                                                                       
Sbjct: 601 VLEIASGLSYLHEECETQIIHCDIKPQNILLDHNYIVKISDFGIAKLMKKDQTRTDTMMR 660

BLAST of HG10012672 vs. NCBI nr
Match: XP_022137776.1 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 [Momordica charantia])

HSP 1 Score: 741.9 bits (1914), Expect = 4.6e-210
Identity = 395/660 (59.85%), Postives = 448/660 (67.88%), Query Frame = 0

Query: 125 MSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQ 184
           MS++GN MLL+SSSNP+WQSFDHPTDTLLPGQ LR+GQ+L+SNANGTVDYSTG+FML+VQ
Sbjct: 1   MSDDGNLMLLDSSSNPVWQSFDHPTDTLLPGQVLRMGQKLYSNANGTVDYSTGQFMLDVQ 60

Query: 185 GFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTV 244
             DGNVIMSAFR+ DPAYK+TGT++ KN+TIVFN+TTA LYV+N TT IY  ++  QL V
Sbjct: 61  S-DGNVIMSAFRYPDPAYKYTGTINYKNSTIVFNKTTALLYVLNDTTTIY--STLTQLPV 120

Query: 245 PVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQ 304
           PV DYYHRATVDD+GNFQQLIRIK+GS GEWRSVWKFVE PCMVSNICGVFGFCT+ DNQ
Sbjct: 121 PVKDYYHRATVDDKGNFQQLIRIKSGS-GEWRSVWKFVERPCMVSNICGVFGFCTSNDNQ 180

Query: 305 KVNCECLEGYSPIDPNIPSKGCYPNKIMDFCSDSNFKMVKLENVDFPFLK-DNSDATMVR 364
             NCECLEGYSPIDPN+PSKGCYPN  +DFCS+S+FK+VKLEN DFPF K + SDATMV 
Sbjct: 181 NANCECLEGYSPIDPNVPSKGCYPNLAVDFCSNSDFKIVKLENADFPFFKVEESDATMVA 240

Query: 365 ATDANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVY 424
           + D ++CEEV+RN+C RTAAVYF+GACYKKRMPLLNAR +IP+TNNHVAFLKVPTINNV 
Sbjct: 241 SKDVDQCEEVVRNDCLRTAAVYFNGACYKKRMPLLNARRSIPDTNNHVAFLKVPTINNVN 300

Query: 425 S--KKSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFS 484
              +KSPS EALLAIFV+CST  V+F AMA+YYHP  KGF QRKKP KPKPL++NLKVFS
Sbjct: 301 GERRKSPSNEALLAIFVLCSTLVVLFTAMAVYYHP--KGFLQRKKPAKPKPLEMNLKVFS 360

Query: 485 FNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVI 544
           FNEL+EATNGFKN+LGRGAFG VYNGVLML+DQ+VEVAVKQLEK+ EQGEKEFITEVQVI
Sbjct: 361 FNELREATNGFKNKLGRGAFGTVYNGVLMLSDQQVEVAVKQLEKLFEQGEKEFITEVQVI 420

Query: 545 GLTHHKNLVRLFCFCNE------------------------------------------- 604
           GLTHH+NLVRL  FCNE                                           
Sbjct: 421 GLTHHRNLVRLLGFCNEGDHRLLVYELMKNGPLSNFLFGEKENQKPKWESRAEIVMEIAR 480

Query: 605 ------------------------------------------------------------ 625
                                                                       
Sbjct: 481 GLSYLHEECETQIIHCAQNILLDDNYSAKISDFGLAKLMKKNQTRTATMIRGTMGYMAPE 540

BLAST of HG10012672 vs. NCBI nr
Match: KAF3953154.1 (hypothetical protein CMV_021374 [Castanea mollissima])

HSP 1 Score: 684.5 bits (1765), Expect = 8.7e-193
Identity = 354/686 (51.60%), Postives = 460/686 (67.06%), Query Frame = 0

Query: 1   MAAIELLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIV 60
           MA   +L  S    L      AQ Q  N+T KLGSSI AGS+  W+S SGDFAFGF+ +V
Sbjct: 1   MAVTRILLWSFSFILALLCSSAQIQ--NSTTKLGSSITAGSDSSWKSPSGDFAFGFYHLV 60

Query: 61  DGRYLVGIVFDKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTT 120
             +YLVGI FDK+ E+TL WSANRDDPA   STI L   GQ VL H N TQ+ I N T+T
Sbjct: 61  TNQYLVGIWFDKISEKTLVWSANRDDPAGKGSTIKLLLNGQLVLTHTNGTQLFICNHTST 120

Query: 121 SSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFM 180
            SA M N+GNF+L NSSS  IWQSFD PTDT+L GQ L +GQ+L SNANGTVDYSTG++ 
Sbjct: 121 KSASMQNDGNFVLRNSSSGIIWQSFDFPTDTILLGQTLVMGQKLHSNANGTVDYSTGQYQ 180

Query: 181 LEVQGFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYV--VNGTTIIYPMTS 240
           LE+Q  DGN+++SAFRF+ PAY F+ T  N N ++VFN+TTAF+Y   V   ++ Y MTS
Sbjct: 181 LEIQQLDGNILLSAFRFSGPAYWFSDTAGNGNVSLVFNKTTAFMYAIYVEKNSMAYNMTS 240

Query: 241 QAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFC 300
             ++  P+ DYYH+AT++D+GNFQQL  +K     +W  VW+ +  PC V NICGV+GFC
Sbjct: 241 --EVPTPIEDYYHKATINDKGNFQQLAFLKRNGASKWNVVWEAIIKPCTVDNICGVYGFC 300

Query: 301 TTIDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFCSDS----NFKMVKLENVDFPFLK 360
           TT D++ VNC CL GYSP+DPN PSKGCYPN +MDFC+ +    NF +  L   D P   
Sbjct: 301 TTPDDKIVNCTCLPGYSPLDPNTPSKGCYPNVVMDFCAPNSSALNFTIQTLNTADNP-NH 360

Query: 361 DNSDATMVRATDANECEEVLRNECFRTAAVYFDG--ACYKKRMPLLNARGNIPNTNNHVA 420
           D +D   +++TDA++C++ + ++CF  AAV+  G   CYKKRMPL+N+R + P TNN V 
Sbjct: 361 DFADLARIQSTDADKCKKEVMDDCFCVAAVFNSGNNVCYKKRMPLVNSRRSSPATNNMVV 420

Query: 421 FLKVPTINNVY----SKKSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFF--QRKK 480
           F+KVP  N+       K+SPS   LLA F+ CSTFAV+F A+AIY+HP+ + +   Q + 
Sbjct: 421 FIKVPVENHDLGPKDKKESPSSAVLLASFLSCSTFAVLFAAIAIYHHPLAQRYIRVQLQP 480

Query: 481 PTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKM 540
           P K KP+++NLK +SF +L+EATNGFKN LG+GAFG VY+GVL L D+EVEVAVK+LEK+
Sbjct: 481 PPKRKPVELNLKAYSFQDLREATNGFKNMLGQGAFGTVYSGVLTLEDEEVEVAVKKLEKV 540

Query: 541 IEQGEKEFITEVQVIGLTHHKNLVRLFCFCNEE--------------------------- 600
           IE+GEKEF+TEVQVIGLTHH+NLVRL  +CNE+                           
Sbjct: 541 IERGEKEFLTEVQVIGLTHHRNLVRLLGYCNEQSHRLLVYELMKNGTLSNFLKHMELHPA 600

Query: 601 ----------------------RLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRP 624
                                  L   +SHDSE +++Y RFER+ +VG+WC C NP LRP
Sbjct: 601 DESMDVEYTILTDWVLSCVRAANLEATVSHDSELLSDYKRFERMALVGVWCTCSNPTLRP 660

BLAST of HG10012672 vs. NCBI nr
Match: KAF3957004.1 (hypothetical protein CMV_017943 [Castanea mollissima])

HSP 1 Score: 670.2 bits (1728), Expect = 1.7e-188
Identity = 345/641 (53.82%), Postives = 448/641 (69.89%), Query Frame = 0

Query: 21  YAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVFDKVPERTLAW 80
           ++ +Q  N TI LGSSI AGSN  W+SSSGDFAFGF+ +V G+YLVGI FDK+ E+TL W
Sbjct: 19  FSCSQINNGTIDLGSSISAGSNSSWKSSSGDFAFGFYPLVTGKYLVGIWFDKISEKTLVW 78

Query: 81  SANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAFMSNNGNFMLLNSSSNP 140
           S+NRDDPA   S+I L  TGQ VL H+N T++ IYNGT+T+SA M ++GNF+L NSSS  
Sbjct: 79  SSNRDDPAGKGSSIKLTLTGQLVLTHSNGTELFIYNGTSTNSATMKDDGNFVLQNSSSGI 138

Query: 141 IWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADP 200
           IWQSF+ PTDT+L GQAL +GQ+L+SNAN TVDYSTGR+ LE+Q  DGNV++ AFRF+DP
Sbjct: 139 IWQSFNFPTDTILLGQALVMGQKLYSNANDTVDYSTGRYTLEIQELDGNVLLYAFRFSDP 198

Query: 201 AYKFTGTVDNKNATIVFNQTTAFLYV--VNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQ 260
            Y +T T    N ++VFN  TAF+YV  V   +  + MTS   + +P++DYYHRAT++D+
Sbjct: 199 PYWYTAT--KGNVSLVFNPATAFMYVISVEDNSTAFNMTS--TVPIPISDYYHRATINDK 258

Query: 261 GNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPID 320
           GNFQQL   K     +W  VW+ +  PC V NICGVFGFCT+ DN+ VNC CL GYSP D
Sbjct: 259 GNFQQLAFYKGNGASQWDVVWEAISQPCTVDNICGVFGFCTSPDNKMVNCTCLPGYSPWD 318

Query: 321 PNIPSKGCYPNKIMDFCSD----SNFKMVKLENVDFPFLKDNSDATMVRATDANE--CEE 380
           PNIPSKGCYPN +MD+C+     SNF + +L++ D P   ++  A + +    NE  C +
Sbjct: 319 PNIPSKGCYPNVVMDYCAPNSLASNFTIERLDDSDVP---NSYFANLAKIEQTNEDGCRK 378

Query: 381 VLRNECFRTAAVYFDGA---CYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYSKK--- 440
            + ++CF  A V F+GA   CYKKRMPL+NAR + P TNN V FLKVP  +N +S+K   
Sbjct: 379 EVMDDCFCVAGV-FNGAENVCYKKRMPLVNARRSNPATNNMVTFLKVPVEDNSWSRKNKK 438

Query: 441 -SPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNEL 500
            SPS   LLA F+ CS  AV+F A+AIY+HP+ + + + + P K KP+++NLK FSF +L
Sbjct: 439 ESPSSAVLLASFLSCSVLAVLFAAIAIYHHPLAQRYIRVQPPPKRKPVELNLKAFSFQDL 498

Query: 501 KEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTH 560
           + ATNGF+N LG+GAFG VY+G L + D+EVEVAVK+LEK+IEQGEKEF+TEVQVI LTH
Sbjct: 499 QGATNGFENILGQGAFGTVYSGFLTIEDEEVEVAVKKLEKVIEQGEKEFLTEVQVIALTH 558

Query: 561 HKNLVRL-----------------------FCFCNEERLRDIISHDSEAMNEYGRFERIT 620
           HKNLVRL                               L   +SHD E +++Y RFER+ 
Sbjct: 559 HKNLVRLKHMELHPGDESMEVEYTILTDWILSCVRAGNLETTVSHDFEVLSDYKRFERMV 618

Query: 621 MVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPPLVETDAY 624
           MVG+WCIC NP LRPSMK+V+ MLEG++EV +PPL +   +
Sbjct: 619 MVGVWCICSNPTLRPSMKKVVQMLEGTVEVDVPPLNDAQMF 651

BLAST of HG10012672 vs. NCBI nr
Match: KAF3947158.1 (hypothetical protein CMV_026671 [Castanea mollissima])

HSP 1 Score: 665.6 bits (1716), Expect = 4.2e-187
Identity = 342/661 (51.74%), Postives = 441/661 (66.72%), Query Frame = 0

Query: 24  TQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVFDKVPERTLAWSAN 83
           TQ  N+TI LGSSI AGSN  W+S SG FAFGF+ +V G+YLVGI FDK+ E+TL WSAN
Sbjct: 22  TQMNNSTINLGSSISAGSNSSWKSPSGHFAFGFYPLVTGKYLVGIWFDKISEKTLVWSAN 81

Query: 84  RDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAFMSNNGNFMLLNSSSNPIWQ 143
           RDDPA   S+I L  TGQ  L H+N T+V IYNGT+T SA M ++GNF+L NSSS  IWQ
Sbjct: 82  RDDPAGKGSSIKLSRTGQLALTHSNGTEVVIYNGTSTDSASMQDDGNFVLQNSSSGIIWQ 141

Query: 144 SFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYK 203
           SF+ PT+T+L GQAL +GQ+L+SNAN TVDYSTGR+ LE+Q  DGNV++ AF F+ PAY 
Sbjct: 142 SFNFPTNTILLGQALVMGQKLYSNANDTVDYSTGRYALEIQELDGNVLLKAFHFSTPAY- 201

Query: 204 FTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQ 263
               +   N ++VFN+TTAF++V++             +  P+NDYYHRAT++D+GNFQQ
Sbjct: 202 -WNAITKGNVSLVFNRTTAFMHVISVEDNSTVSNMTTTVPTPINDYYHRATINDKGNFQQ 261

Query: 264 LIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPIDPNIPS 323
           L   K     +W  VW+ +  PC+V NICGVFG+CTT D++ VNC CL GY P+DPN PS
Sbjct: 262 LAFPKANGASQWIVVWEAITKPCIVDNICGVFGYCTTPDDKIVNCTCLPGYLPLDPNTPS 321

Query: 324 KGCYPNKIMDFC----SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECF 383
           KGCYPN +MDFC    S SNF +  L+NVDFP   D ++   +  TD + C + + N+CF
Sbjct: 322 KGCYPNVVMDFCASNSSASNFTIGSLDNVDFP-NADFANLAKIEQTDEDGCRKEVMNDCF 381

Query: 384 RTAAVYF--DGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYS----KKSPSKEAL 443
             A V++    ACYKKRMPL+N R + P TNN VAFLKVP  NN  +    K+SPS   L
Sbjct: 382 CVAGVFYRDTQACYKKRMPLVNGRISNPTTNNMVAFLKVPVENNNLNHKDKKESPSSAVL 441

Query: 444 LAIFVVCSTFAVVFIAMAIYYHPITKGFF--QRKKPTKPKPLDVNLKVFSFNELKEATNG 503
           LA F+ CS  AV+F A+ I  HP  + +   Q + P K KP+++NLK +SF++L+EA+NG
Sbjct: 442 LASFLACSILAVLFAAIGICQHPWAQRYIRVQLQPPPKRKPVEINLKAYSFHDLQEASNG 501

Query: 504 FKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVR 563
           FKN LG+GAFG VY+GVL L D+EVEVAVK+LEK+IE+GEKEF+TEVQVIGLTHHKNLVR
Sbjct: 502 FKNMLGQGAFGTVYSGVLTLEDEEVEVAVKKLEKVIERGEKEFLTEVQVIGLTHHKNLVR 561

Query: 564 LFCFCNEE-------------------------------------------------RLR 623
           L  +CNE                                                   L 
Sbjct: 562 LLGYCNEHNHRLLVYELMQNGTLSNFLKHMELHPADESAEVEYTILTDWVLSCVRDGNLE 621

BLAST of HG10012672 vs. ExPASy Swiss-Prot
Match: Q25AG3 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sativa subsp. indica OX=39946 GN=LECRK3 PE=3 SV=2)

HSP 1 Score: 241.1 bits (614), Expect = 3.3e-62
Identity = 208/610 (34.10%), Postives = 302/610 (49.51%), Query Frame = 0

Query: 9   LSLPLF-LCFFHCYAQTQYPNNTIKLGSSII-AGSNEFWQSSSGDFAFGFHQIVDGR--- 68
           L LP+  L   +C    Q   N I +GSS+   G N  W S S DFAFGF + VDG    
Sbjct: 5   LFLPILQLLLLYCTKSAQAQLN-ISIGSSLTPQGVNNSWISPSADFAFGF-RAVDGNSSS 64

Query: 69  YLVGIVFDKVPERTLAW-----SANRDD--PAKANSTIILK-PTGQFVLIHANNTQVSIY 128
           YL+ + F+K+ ++T+ W     S  +DD  P +  S  +LK   G   L   +  +V   
Sbjct: 65  YLLAVWFNKIADKTVVWYARTSSNGKDDTIPVQVQSGSVLKLADGALSLRDPSGNEVWNP 124

Query: 129 NGTTTSSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYS 188
             T    A M + GNF LL +     W+SF  P+DT+LP Q L +G  L S    T DYS
Sbjct: 125 QVTDVGYARMLDTGNFRLLGTDGATKWESFGDPSDTILPTQVLSLGTALHSRLLAT-DYS 184

Query: 189 TGRFMLEVQGFDGNVIM------SAFRFADPAYKFTGTVDNKNATIVFNQT-TAFLYVVN 248
            GRF L+VQ  DGN++M      S + + DP Y  + TVDN  + +VFN+T   +  ++N
Sbjct: 185 NGRFQLKVQR-DGNLVMYPDAVPSGYLY-DP-YWASNTVDN-GSQLVFNETGRIYFTIIN 244

Query: 249 GTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSG-----GEWRSVWKFVE 308
           G+ +   +TS       + D++HRAT+D  G F+Q +  KN         +W +V    E
Sbjct: 245 GSQV--NITSAG--VDSMGDFFHRATLDTDGVFRQYVYPKNIHARPLWPEQWTAVDVLPE 304

Query: 309 NPC------MVSNICGVFGFCTTIDNQK--VNCECLEGYSPIDPNIPSKGCYPNKIMDFC 368
           N C      + S  CG   +C TID  K   +C C + Y  ID     KGC P+     C
Sbjct: 305 NICQSIQTMVGSGACGFNSYC-TIDGTKNTTSCLCPQNYKFIDDKRKYKGCRPDFEPQNC 364

Query: 369 ------SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVY--FD 428
                 +   + M  ++ VD+P     SD       D  EC  +   +CF   AV+    
Sbjct: 365 DLDETTAMLQYDMAPIDRVDWPL----SDYEQYNPIDQTECRRLCVIDCFCAVAVFDKAS 424

Query: 429 GACYKKRMPLLNAR--GNIPNTNNHVAFLKVPTINN---VYS------KKSPSKEALLAI 488
             C+KKR PL N +   N+P T      +KVP   N   V+S      K+      L + 
Sbjct: 425 STCWKKRFPLSNGKMDVNVPRT----VLIKVPRSTNSPSVFSSGSSKWKEDKKYWILGSS 484

Query: 489 FVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLD---VNLKVFSFNELKEATNGFK 548
            +  S+  V F+ +++           RKK    +P +   +  K+F+++EL++AT GF+
Sbjct: 485 LLFGSSVLVNFLLISVMLFGTYCSITSRKKIQLSQPSNKSGLPPKIFTYSELEKATGGFQ 544

Query: 549 NQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLF 562
             LG GA G VY G L  ++  + +AVK++EK+ ++ +KEF+ EVQ IG T H+NLVRL 
Sbjct: 545 EVLGTGASGVVYKGQLQ-DEFGINIAVKKIEKLQQEAQKEFLVEVQTIGQTFHRNLVRLL 593


HSP 2 Score: 51.2 bits (121), Expect = 4.9e-05
Identity = 20/57 (35.09%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 560 RLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP 617
           R+  +++ D EA+    + ER   V LWC+   P++RP+M +V  ML+G++++  PP
Sbjct: 745 RIDLLVAGDDEAIFNIKKVERFVAVALWCLQEEPSMRPTMLKVTQMLDGAVQIPTPP 801

BLAST of HG10012672 vs. ExPASy Swiss-Prot
Match: Q0JEU6 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sativa subsp. japonica OX=39947 GN=LECRK3 PE=3 SV=2)

HSP 1 Score: 240.7 bits (613), Expect = 4.3e-62
Identity = 208/610 (34.10%), Postives = 300/610 (49.18%), Query Frame = 0

Query: 9   LSLPLF-LCFFHCYAQTQYPNNTIKLGSSII-AGSNEFWQSSSGDFAFGFHQIVDGR--- 68
           L LP+  L   +C    Q   N I +GSS+   G N  W S S DFAFGF   VDG    
Sbjct: 5   LFLPILQLLLLYCTKSAQAQLN-ISIGSSLTPQGVNNSWISPSADFAFGF-LAVDGNSSS 64

Query: 69  YLVGIVFDKVPERTLAW-----SANRDD--PAKANSTIILK-PTGQFVLIHANNTQVSIY 128
           YL+ + F+K+ ++T+ W     S  +DD  P +  S  +LK   G   L   +  +V   
Sbjct: 65  YLLAVWFNKIADKTVVWYARTSSNGKDDTIPVQVQSGSVLKLADGALSLRDPSGNEVWNP 124

Query: 129 NGTTTSSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYS 188
             T    A M + GNF LL +     W+SF  P+DT+LP Q L +G  L S    T DYS
Sbjct: 125 QVTDVGYARMLDTGNFRLLGTDGATKWESFGDPSDTILPTQVLSLGTALHSRLLAT-DYS 184

Query: 189 TGRFMLEVQGFDGNVIM------SAFRFADPAYKFTGTVDNKNATIVFNQT-TAFLYVVN 248
            GRF L+VQ  DGN++M      S + + DP Y  + TVDN  + +VFN+T   +  ++N
Sbjct: 185 NGRFQLKVQR-DGNLVMYPDAVPSGYLY-DP-YWASNTVDN-GSQLVFNETGRIYFTIIN 244

Query: 249 GTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSG-----GEWRSVWKFVE 308
           G+ +   +TS       + D++HRAT+D  G F+Q +  KN         +W +V    E
Sbjct: 245 GSQV--NITSAG--VDSMGDFFHRATLDTDGVFRQYVYPKNIHARPLWPEQWTAVDVLPE 304

Query: 309 NPC------MVSNICGVFGFCTTIDNQK--VNCECLEGYSPIDPNIPSKGCYPNKIMDFC 368
           N C      + S  CG   +C TID  K   +C C + Y  ID     KGC P+     C
Sbjct: 305 NICQSIQTMVGSGACGFNSYC-TIDGTKNTTSCLCPQNYKFIDDKRKYKGCRPDFEPQNC 364

Query: 369 ------SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVY--FD 428
                 +   + M  ++ VD+P     SD       D  EC  +   +CF   AV+    
Sbjct: 365 DLDETTAMLQYDMAPIDRVDWPL----SDYEQYNPIDQTECRRLCVTDCFCAVAVFDKAS 424

Query: 429 GACYKKRMPLLNAR--GNIPNTNNHVAFLKVPTINN---VYS------KKSPSKEALLAI 488
             C+KKR PL N +   N+P T      +KVP   N   V+S      K+      L + 
Sbjct: 425 STCWKKRFPLSNGKMDVNVPRT----VLIKVPRSTNSPSVFSSGSSKWKEDQKYWILGSS 484

Query: 489 FVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLD---VNLKVFSFNELKEATNGFK 548
            +  S+  V F+ +++           RKK    +P +   +  K+F+++EL++AT GF+
Sbjct: 485 LLFGSSVLVNFLLISVMLFGTYCSITSRKKTQLSQPSNNSGLPPKIFTYSELEKATGGFQ 544

Query: 549 NQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLF 562
             LG GA G VY G L  ++    +AVK++EK+ ++ +KEF+ EVQ IG T H+NLVRL 
Sbjct: 545 EVLGTGASGVVYKGQLQ-DEFGTNIAVKKIEKLQQEAQKEFLVEVQTIGQTFHRNLVRLL 593


HSP 2 Score: 51.2 bits (121), Expect = 4.9e-05
Identity = 20/57 (35.09%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 560 RLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP 617
           R+  +++ D EA+    + ER   V LWC+   P++RP+M +V  ML+G++++  PP
Sbjct: 745 RIDLLVAGDDEAIFNIKKVERFVAVALWCLQEEPSMRPTMLKVTQMLDGAVQIPTPP 801

BLAST of HG10012672 vs. ExPASy Swiss-Prot
Match: Q25AG2 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sativa subsp. indica OX=39946 GN=LECRK4 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.7e-61
Identity = 203/600 (33.83%), Postives = 294/600 (49.00%), Query Frame = 0

Query: 6   LLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIA-GSNEFWQSSSGDFAFGFHQIVDGR- 65
           L  LSL L +      AQ Q     I LG+S+   G N  W S SGDFAFGF  I DG  
Sbjct: 5   LFLLSLQLLVLLSSPSAQAQ----NISLGTSLTTQGPNNAWLSPSGDFAFGFRPI-DGNS 64

Query: 66  --YLVGIVFDKVPERTLAWSANRDD----PAKANSTIILKPTGQFVLIHANNTQVSIYNG 125
             YL+ I F+K+ ++T  W A   +    P +  S  IL+ T   VL   + T   ++N 
Sbjct: 65  SFYLLAIWFNKISDKTATWYAKTSEQEPQPIQVPSGSILQFTSTGVLSLRDPTNREVWNP 124

Query: 126 TTTSS--AFMSNNGNFMLLNSSSNPI-WQSFDHPTDTLLPGQALRIGQRLFSNANGTVDY 185
             T +  A M + GNF++  +  + I W++F +PTDT+L  QAL  G +L S    T DY
Sbjct: 125 GATGAPYASMLDTGNFVIAAAGGSTISWETFKNPTDTILVTQALSPGMKLRSRLL-TTDY 184

Query: 186 STGRFMLEVQGFDGNVIMSAF---RFADPAYK--FTGTVDNKNATIVFNQTTAFLYVVNG 245
           S GRF+L ++     +   A       DP +       V N+   +VFN TT  +YV   
Sbjct: 185 SNGRFLLNMETQRAALYTMAVPSGNLYDPYWSTPIDENVTNQVTNLVFN-TTGRIYVSMK 244

Query: 246 TTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGS--GGEWRSVWKFVENPC- 305
               + MTS   +   + DYYHRAT+D  G F+Q +  K  S     W +V    EN C 
Sbjct: 245 NGTQFNMTS--GVIRSMEDYYHRATLDPDGVFRQYVYPKKPSSMSQAWTAVSIQPENICN 304

Query: 306 ----MVSNICGVFGFCT-TIDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFC------ 365
               + S  CG   +C     N + +C C E YS  D     +GC P+  +  C      
Sbjct: 305 AQTKVGSGTCGFNSYCMFDGSNNQTSCVCPEQYSFFDEVRKYRGCRPDFELQSCDLDEAA 364

Query: 366 SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVYFDGACYKKRM 425
           S + ++   + NVD+P     +D       D +EC  +   +CF   AV+ +  C+KK++
Sbjct: 365 SMAQYEFNLVNNVDWP----QADYEWYTPIDMDECRRLCLIDCFCAVAVFHENTCWKKKL 424

Query: 426 PLLNARGNIPNTNNHVAFLKVPTINNVYSKKSPSKE------------ALLAIFVVCSTF 485
           PL N  G + +       +KVP  N+   +   S++            +LL    V + F
Sbjct: 425 PLSN--GIMGSGVQRTVLIKVPKSNSSQPELRKSRKWKSDKKLWILGSSLLLGGSVIANF 484

Query: 486 AVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGR 545
           A+  + +   Y  IT+   Q  +P++   L   LK FS+ EL++AT+GFK  LG GA G 
Sbjct: 485 ALSSVLLFGTYCTITRKDVQPLQPSRDPGLP--LKAFSYAELEKATDGFKEVLGTGASGI 544

Query: 546 VYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNE--ERL 562
           VY G L  ++    +AVK+++K+  + EKEF  EVQ IG T+HKNLVR+  FCNE  ERL
Sbjct: 545 VYKGQLQ-DELGTYIAVKKIDKIQHETEKEFAVEVQTIGRTYHKNLVRMLGFCNEGTERL 586


HSP 2 Score: 45.4 bits (106), Expect = 2.7e-03
Identity = 19/53 (35.85%), Postives = 29/53 (54.72%), Query Frame = 0

Query: 564 IISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP 617
           ++  D EA     + ER   V LWC+   P +RPS+ +V  ML+G+  +  PP
Sbjct: 742 LVDGDDEAKLNIKKVERFVAVALWCLQEEPTMRPSILKVTQMLDGADAIPTPP 794

BLAST of HG10012672 vs. ExPASy Swiss-Prot
Match: Q7FAZ0 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sativa subsp. japonica OX=39947 GN=LECRK4 PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.7e-61
Identity = 203/600 (33.83%), Postives = 294/600 (49.00%), Query Frame = 0

Query: 6   LLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIA-GSNEFWQSSSGDFAFGFHQIVDGR- 65
           L  LSL L +      AQ Q     I LG+S+   G N  W S SGDFAFGF  I DG  
Sbjct: 5   LFLLSLQLLVLLSSPSAQAQ----NISLGTSLTTQGPNNAWLSPSGDFAFGFRPI-DGNS 64

Query: 66  --YLVGIVFDKVPERTLAWSANRDD----PAKANSTIILKPTGQFVLIHANNTQVSIYNG 125
             YL+ I F+K+ ++T  W A   +    P +  S  IL+ T   VL   + T   ++N 
Sbjct: 65  SFYLLAIWFNKISDKTATWYAKTSEQEPQPIQVPSGSILQFTSTGVLSLRDPTNREVWNP 124

Query: 126 TTTSS--AFMSNNGNFMLLNSSSNPI-WQSFDHPTDTLLPGQALRIGQRLFSNANGTVDY 185
             T +  A M + GNF++  +  + I W++F +PTDT+L  QAL  G +L S    T DY
Sbjct: 125 GATGAPYASMLDTGNFVIAAAGGSTISWETFKNPTDTILVTQALSPGMKLRSRLL-TTDY 184

Query: 186 STGRFMLEVQGFDGNVIMSAF---RFADPAYK--FTGTVDNKNATIVFNQTTAFLYVVNG 245
           S GRF+L ++     +   A       DP +       V N+   +VFN TT  +YV   
Sbjct: 185 SNGRFLLNMETQRAALYTMAVPSGNLYDPYWSTPIDENVTNQVTNLVFN-TTGRIYVSMK 244

Query: 246 TTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGS--GGEWRSVWKFVENPC- 305
               + MTS   +   + DYYHRAT+D  G F+Q +  K  S     W +V    EN C 
Sbjct: 245 NGTQFNMTS--GVIRSMEDYYHRATLDPDGVFRQYVYPKKPSSMSQAWTAVSIQPENICN 304

Query: 306 ----MVSNICGVFGFCT-TIDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFC------ 365
               + S  CG   +C     N + +C C E YS  D     +GC P+  +  C      
Sbjct: 305 AQTKVGSGTCGFNSYCMFDGSNNQTSCVCPEQYSFFDEVRKYRGCRPDFELQSCDLDEAA 364

Query: 366 SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVYFDGACYKKRM 425
           S + ++   + NVD+P     +D       D +EC  +   +CF   AV+ +  C+KK++
Sbjct: 365 SMAQYEFNLVNNVDWP----QADYEWYTPIDMDECRRLCLIDCFCAVAVFHENTCWKKKL 424

Query: 426 PLLNARGNIPNTNNHVAFLKVPTINNVYSKKSPSKE------------ALLAIFVVCSTF 485
           PL N  G + +       +KVP  N+   +   S++            +LL    V + F
Sbjct: 425 PLSN--GIMGSGVQRTVLIKVPKSNSSQPELRKSRKWKSDKKLWILGSSLLLGGSVIANF 484

Query: 486 AVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGR 545
           A+  + +   Y  IT+   Q  +P++   L   LK FS+ EL++AT+GFK  LG GA G 
Sbjct: 485 ALSSVLLFGTYCTITRKDVQPLQPSRDPGLP--LKAFSYAELEKATDGFKEVLGTGASGI 544

Query: 546 VYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNE--ERL 562
           VY G L  ++    +AVK+++K+  + EKEF  EVQ IG T+HKNLVR+  FCNE  ERL
Sbjct: 545 VYKGQLQ-DELGTYIAVKKIDKIQHETEKEFAVEVQTIGRTYHKNLVRMLGFCNEGTERL 586


HSP 2 Score: 45.4 bits (106), Expect = 2.7e-03
Identity = 19/53 (35.85%), Postives = 29/53 (54.72%), Query Frame = 0

Query: 564 IISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP 617
           ++  D EA     + ER   V LWC+   P +RPS+ +V  ML+G+  +  PP
Sbjct: 742 LVDGDDEAKLNIKKVERFVAVALWCLQEEPTMRPSILKVTQMLDGADAIPTPP 794

BLAST of HG10012672 vs. ExPASy Swiss-Prot
Match: Q7FAZ2 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 OS=Oryza sativa subsp. japonica OX=39947 GN=LECRK2 PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.1e-60
Identity = 206/606 (33.99%), Postives = 296/606 (48.84%), Query Frame = 0

Query: 9   LSLPLF-LCFFHCYAQTQYPNNTIKLGSSII-AGSNEFWQSSSGDFAFGFHQIVDGR--- 68
           L LP+  L   +C    Q   N I +GSS+   G N  W S + DFAFGF   VDG    
Sbjct: 5   LFLPILQLLLLYCTKSAQAQLN-ISIGSSLTPQGINNSWISPTADFAFGF-LAVDGNSSS 64

Query: 69  YLVGIVFDKVPERTLAW----SANRDD---PAKANSTIILK-PTGQFVLIHANNTQVSIY 128
           YL+ + F+K+ ++T+ W    S+NR D   P +  +  ILK   G   L   +  +V   
Sbjct: 65  YLLAVWFNKIADKTVIWYAKTSSNRQDDTIPIQVQAGSILKLADGALSLRDPSGNEVWNP 124

Query: 129 NGTTTSSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYS 188
             T    A M + GNF LL +     W+SF  P+DT+LP Q L +G  L S    T DYS
Sbjct: 125 RVTDVGYARMLDTGNFRLLGTDGATKWESFGDPSDTILPTQVLPLGTALHSRLLAT-DYS 184

Query: 189 TGRFMLEVQGFDGNVIM------SAFRFADPAYKFTGTVDNKNATIVFNQT-TAFLYVVN 248
            GRF L VQ  DGN+++      SA+ + DP Y  + TV N  + +VFN+T   +  + N
Sbjct: 185 NGRFQLNVQD-DGNLVLYLVAVPSAY-YHDP-YWASNTVGN-GSQLVFNETGRIYFTLTN 244

Query: 249 GTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSG-----GEWRSVWKFVE 308
           G+ I   +TS       + D++HRAT+D  G F+Q I  K+         +WR+V    E
Sbjct: 245 GSQI--NITSAG--VDSMGDFFHRATLDTDGVFRQYIYPKSKQARSLWQEQWRAVDALPE 304

Query: 309 NPCMV------SNICGVFGFCTTIDNQK--VNCECLEGYSPIDPNIPSKGCYPNKIMDFC 368
           N C        S  CG   +C T D  K   NC C + Y   D     KGC P+     C
Sbjct: 305 NICQTIQTKVGSGACGFNSYC-TFDGTKNTTNCLCPQRYKFFDNERTYKGCRPDFEPQSC 364

Query: 369 ------SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVYF--D 428
                 +   ++M  ++ +++P     SD       D  EC  +   +CF + AV+    
Sbjct: 365 DLDETAAMVQYEMTPIDRINWPL----SDYEQYSPIDETECRRLCVIDCFCSVAVFNKPS 424

Query: 429 GACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYSK--------KSPSKEALL--AIF 488
             CYKK++PL N  GN+ ++      LKVP   N  S         K   K  +L  ++F
Sbjct: 425 NTCYKKKLPLSN--GNMDSSLQATVLLKVPRSTNSPSMISSGSSKWKKDKKYWILGSSLF 484

Query: 489 V---VCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNL--KVFSFNELKEATNGF 548
               V   F ++F+ +   Y  IT    ++K      P +  L  K+F++ EL++AT GF
Sbjct: 485 FGSSVLVNFLLIFVLLFGTYCSITS---RKKTQLSQLPSNSGLPSKIFTYRELEKATGGF 544

Query: 549 KNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRL 559
              LG GA G VY G L  ++    +AVK++EK+ ++ +KEF+ EVQ IG T H+NLVRL
Sbjct: 545 HEVLGTGASGIVYKGQLQ-DECGTNIAVKKIEKLQQEAQKEFLVEVQTIGQTFHRNLVRL 588


HSP 2 Score: 53.1 bits (126), Expect = 1.3e-05
Identity = 20/57 (35.09%), Postives = 37/57 (64.91%), Query Frame = 0

Query: 560 RLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP 617
           R+  +++ D EA+    + ER   V LWC+   P++RP+M +V+ ML+G++++  PP
Sbjct: 745 RIDLLVASDDEAIFNIKKVERFVAVALWCLQEEPSMRPTMHKVMQMLDGAVQIPTPP 801

BLAST of HG10012672 vs. ExPASy TrEMBL
Match: A0A6J1C7P5 (G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 OS=Momordica charantia OX=3673 GN=LOC111009136 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 2.2e-210
Identity = 395/660 (59.85%), Postives = 448/660 (67.88%), Query Frame = 0

Query: 125 MSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQ 184
           MS++GN MLL+SSSNP+WQSFDHPTDTLLPGQ LR+GQ+L+SNANGTVDYSTG+FML+VQ
Sbjct: 1   MSDDGNLMLLDSSSNPVWQSFDHPTDTLLPGQVLRMGQKLYSNANGTVDYSTGQFMLDVQ 60

Query: 185 GFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTV 244
             DGNVIMSAFR+ DPAYK+TGT++ KN+TIVFN+TTA LYV+N TT IY  ++  QL V
Sbjct: 61  S-DGNVIMSAFRYPDPAYKYTGTINYKNSTIVFNKTTALLYVLNDTTTIY--STLTQLPV 120

Query: 245 PVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQ 304
           PV DYYHRATVDD+GNFQQLIRIK+GS GEWRSVWKFVE PCMVSNICGVFGFCT+ DNQ
Sbjct: 121 PVKDYYHRATVDDKGNFQQLIRIKSGS-GEWRSVWKFVERPCMVSNICGVFGFCTSNDNQ 180

Query: 305 KVNCECLEGYSPIDPNIPSKGCYPNKIMDFCSDSNFKMVKLENVDFPFLK-DNSDATMVR 364
             NCECLEGYSPIDPN+PSKGCYPN  +DFCS+S+FK+VKLEN DFPF K + SDATMV 
Sbjct: 181 NANCECLEGYSPIDPNVPSKGCYPNLAVDFCSNSDFKIVKLENADFPFFKVEESDATMVA 240

Query: 365 ATDANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVY 424
           + D ++CEEV+RN+C RTAAVYF+GACYKKRMPLLNAR +IP+TNNHVAFLKVPTINNV 
Sbjct: 241 SKDVDQCEEVVRNDCLRTAAVYFNGACYKKRMPLLNARRSIPDTNNHVAFLKVPTINNVN 300

Query: 425 S--KKSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFS 484
              +KSPS EALLAIFV+CST  V+F AMA+YYHP  KGF QRKKP KPKPL++NLKVFS
Sbjct: 301 GERRKSPSNEALLAIFVLCSTLVVLFTAMAVYYHP--KGFLQRKKPAKPKPLEMNLKVFS 360

Query: 485 FNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVI 544
           FNEL+EATNGFKN+LGRGAFG VYNGVLML+DQ+VEVAVKQLEK+ EQGEKEFITEVQVI
Sbjct: 361 FNELREATNGFKNKLGRGAFGTVYNGVLMLSDQQVEVAVKQLEKLFEQGEKEFITEVQVI 420

Query: 545 GLTHHKNLVRLFCFCNE------------------------------------------- 604
           GLTHH+NLVRL  FCNE                                           
Sbjct: 421 GLTHHRNLVRLLGFCNEGDHRLLVYELMKNGPLSNFLFGEKENQKPKWESRAEIVMEIAR 480

Query: 605 ------------------------------------------------------------ 625
                                                                       
Sbjct: 481 GLSYLHEECETQIIHCAQNILLDDNYSAKISDFGLAKLMKKNQTRTATMIRGTMGYMAPE 540

BLAST of HG10012672 vs. ExPASy TrEMBL
Match: A0A2C9WA55 (Receptor-like serine/threonine-protein kinase OS=Manihot esculenta OX=3983 GN=MANES_03G179900 PE=3 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 3.2e-185
Identity = 356/765 (46.54%), Postives = 458/765 (59.87%), Query Frame = 0

Query: 28  NNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVFDKVPERTLAWSANRDDP 87
           +NTI LGSSI AG+N  W S SGDFAFGF  +++G +LVGI FDK+P+RT AWSANRDDP
Sbjct: 3   SNTIALGSSITAGTNSSWLSPSGDFAFGFFPLLNGLFLVGIWFDKIPQRTRAWSANRDDP 62

Query: 88  AKANSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAFMSNNGNFMLLNSSSNPIWQSFDH 147
           AK  STI L+P GQ VL H+N T+  IYNGT+TSSA M +NGNF+LL+SSS  IWQSFD 
Sbjct: 63  AKIGSTISLRPNGQLVLKHSNGTEYLIYNGTSTSSASMQDNGNFVLLDSSSRIIWQSFDF 122

Query: 148 PTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYKFTGT 207
           PTDT+L GQ L +GQ+L+SNANGTVDYSTGR+ LEVQ  DGN++MSA++FADP Y FT T
Sbjct: 123 PTDTILLGQVLVMGQKLYSNANGTVDYSTGRYKLEVQ-MDGNIVMSAYKFADPGYWFTLT 182

Query: 208 VDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRI 267
             N+N +++FNQ+TA LYV+NGT+II  MT    +  P++DYYHR T++D GN QQ +  
Sbjct: 183 AGNQNVSLIFNQSTALLYVLNGTSIISRMTD--TVPSPIDDYYHRVTINDFGNLQQFVYQ 242

Query: 268 KNGSGGEWRSVWK---FVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPIDPNIPSK 327
           K  +G EWR VW+       PC V NICGV+GFCT+ DN+ VNC+CL GYSP DPN+PSK
Sbjct: 243 KK-NGKEWRVVWEPDFIAAQPCTVYNICGVYGFCTSPDNKTVNCDCLPGYSPWDPNVPSK 302

Query: 328 GCYPNKIMDFC----SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFR 387
           GCYPN +MDFC    S S+F +  ++N DFP     +D   +   D ++C++ + ++CF 
Sbjct: 303 GCYPNVVMDFCAPNSSASDFTIEVIDNADFP-NGQFADMARIEPADFDQCQQEITDDCFA 362

Query: 388 TAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYSK------KSPSKEALL 447
            AAV  +  CYKKRMPLLNAR + P+T+N VAFLKVP +N+          KSPS+ ALL
Sbjct: 363 MAAVLVESVCYKKRMPLLNARRSSPSTDNIVAFLKVPKVNDTNENQDNDRDKSPSRFALL 422

Query: 448 AIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKN 507
           A F++CS  A++F A+AIY+HP+ + +   K+   PKP+++NLK FS+ +L+EATNGF+N
Sbjct: 423 AGFLLCSIMALLFAAIAIYHHPLAQPYLGHKQVPSPKPVEINLKAFSYQQLREATNGFRN 482

Query: 508 QLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFC 567
           +LG+GAFG VY+GVL L D+ V+VAVKQLEK+IEQGEKEF+TEVQVIGLTHH+NLVRL  
Sbjct: 483 KLGKGAFGTVYSGVLNLEDKRVDVAVKQLEKIIEQGEKEFLTEVQVIGLTHHRNLVRLLG 542

Query: 568 FCNEE------------------------------------------------------- 620
           FCNE+                                                       
Sbjct: 543 FCNEQNHRLLVYELMKNGTLANFLFGEETKPSWDKRAEIALGIARGLSYLHEECETQIIH 602

BLAST of HG10012672 vs. ExPASy TrEMBL
Match: A0A5J5C045 (Receptor-like serine/threonine-protein kinase OS=Nyssa sinensis OX=561372 GN=F0562_000322 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 9.4e-185
Identity = 344/723 (47.58%), Postives = 447/723 (61.83%), Query Frame = 0

Query: 25  QYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVFDKVPERTLAWSANR 84
           Q   NTI LGS I AG+N  WQS S DFAFGF+++ +G  LVGI FDK+PE+TL WSANR
Sbjct: 26  QITANTIALGSGITAGTNSSWQSPSADFAFGFYRLANGLLLVGIWFDKIPEKTLVWSANR 85

Query: 85  DDPAKANSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAFMSNNGNFMLLNSSSNPIWQS 144
           DDPA   S I L   GQ VL ++N T+  IYNGT TSSA + ++GN +L +SSS  +W+S
Sbjct: 86  DDPAPVGSAINLTLNGQLVLTYSNGTRYLIYNGTATSSASLQDDGNLVLRDSSSTILWKS 145

Query: 145 FDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYKF 204
           FD PTDT+LPGQ L +GQ+LFSN NGT+D+STGRFMLE+Q  DGNV++SA+RFADP Y F
Sbjct: 146 FDFPTDTILPGQVLVMGQKLFSNTNGTMDFSTGRFMLELQ-MDGNVVISAYRFADPGYWF 205

Query: 205 TGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQL 264
           T T  N+  ++VFNQ+TA +YV+NGT+I Y MT   + T P+ DYYHR T++D GN QQL
Sbjct: 206 TLTAGNQRISLVFNQSTALMYVLNGTSIRYSMTK--KFTTPIKDYYHRTTINDLGNLQQL 265

Query: 265 IRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPIDPNIPSK 324
              K    G W  +W+ +  PC+V NICGVFGFCT++DN+ V C CL GYSP DPN+PSK
Sbjct: 266 AYQKGNDTG-WIIMWEAITEPCLVYNICGVFGFCTSLDNKTVTCTCLPGYSPWDPNVPSK 325

Query: 325 GCYPNKIMDFC----SDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFR 384
           GCYPN + D+C    S S+F +  ++N DFP     +D   + ATD   C + + ++CF 
Sbjct: 326 GCYPNVMKDYCAPNSSASDFTIEAMDNADFP-NGPFADMARIEATDVEGCRKEVMDDCFC 385

Query: 385 TAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTIN------NVYSKKSPSKEALL 444
            A V  +  CYKKRMPLLNAR + P+TNN VAF+KVP +N      N + K+SPS+  LL
Sbjct: 386 IAGVLVESICYKKRMPLLNARRSYPSTNNIVAFIKVPNVNGTLPIPNGHKKESPSRAVLL 445

Query: 445 AIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKN 504
           A  ++CS  A +F A+AIY+HP+T+G+ + + P K KP+++NLK FSF EL++AT+GFK+
Sbjct: 446 AGLLLCSVLAALFAAIAIYHHPLTQGYIRVETPQKQKPVELNLKAFSFRELQDATSGFKH 505

Query: 505 QLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFC 564
           +LG+GAFG VY+G L L D+EV VAVK+LEKMIEQGEKEF+TEV+VIGLTHHKNLVRL  
Sbjct: 506 KLGQGAFGTVYDGALTLEDEEVFVAVKKLEKMIEQGEKEFVTEVRVIGLTHHKNLVRLLG 565

Query: 565 FCNEE------------------------------------------------------- 620
           FCNE                                                        
Sbjct: 566 FCNENNHRLLVYELMKNGTLSSFLFGEGSKPSWEDRAEIALGIARDQSRTSTNFRGTMGY 625

BLAST of HG10012672 vs. ExPASy TrEMBL
Match: A0A6M2F7D4 (Receptor-like serine/threonine-protein kinase OS=Populus davidiana OX=266767 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 2.0e-182
Identity = 357/787 (45.36%), Postives = 457/787 (58.07%), Query Frame = 0

Query: 11  LPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVF 70
           LP+ L F    AQ Q   NTI LG+SI AG+N  W+S SGDFAFGF+ +++G +LVGI F
Sbjct: 22  LPIVLSFLGSSAQPQINTNTINLGASITAGTNSSWRSPSGDFAFGFYPLLNGLFLVGIWF 81

Query: 71  DKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGT-TTSSAFMSNNG 130
           DK+PERTL WSANRDDPA+  STI     GQ VL H+N T   IYNGT   SSA M N+G
Sbjct: 82  DKIPERTLVWSANRDDPARTGSTINFTLDGQLVLTHSNGTGYLIYNGTFGASSALMQNDG 141

Query: 131 NFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGN 190
           NF++  +SS  IW SFD PT+T+L GQ L +GQ+L+SNANGTVDYSTG++MLEVQ  DGN
Sbjct: 142 NFVVKTNSSKVIWGSFDSPTNTILLGQVLVMGQKLYSNANGTVDYSTGQYMLEVQLLDGN 201

Query: 191 VIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDY 250
           V+MSA++FADP Y FT T  N+N +++FNQ+TAF+YVVN T+I Y MTS  Q+  P+ DY
Sbjct: 202 VVMSAYKFADPGYWFTSTQGNQNVSLIFNQSTAFMYVVNHTSIRYNMTS--QVPTPIGDY 261

Query: 251 YHRATVDDQGNFQQLIRIKNGSGGEWRSVWK---FVENPCMVSNICGVFGFCTTIDNQKV 310
           YHRAT++D GN QQ +  K   GG W+ VW+       PC+  NICGV+GFCT+IDN  +
Sbjct: 262 YHRATINDYGNLQQFVYHKENGGG-WKVVWEPDSIKAEPCLPYNICGVYGFCTSIDN-TI 321

Query: 311 NCECLEGYSPIDPNIPSKGCYPNKIMDFC----SDSNFKMVKLENVDFPFLKDNSDATMV 370
           NC CL GYSP DP+IPSKGCYP+ ++DFC    S SNF + K++  DFP  +D +D  +V
Sbjct: 322 NCTCLPGYSPRDPSIPSKGCYPDTVIDFCAPNSSPSNFSLEKIDKADFPNGRDFADMAIV 381

Query: 371 RATDANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINN- 430
              D  EC + + ++CF  A V+ +  CYKKR PLLNAR + P+TNN VAF+K+P  NN 
Sbjct: 382 TEVDEEECRKAIMDDCFAVAGVWVESVCYKKRTPLLNARRSNPSTNNRVAFIKIPKANNN 441

Query: 431 --VYSK--KSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNL 490
             +  K   SPS  ALLA  ++CS   ++F  +AIY+HP+ + +  +K+   PKP+++NL
Sbjct: 442 NQIQDKDDDSPSWIALLAGLLLCSIMTLLFATIAIYHHPLAQPYISQKQLPVPKPVEINL 501

Query: 491 KVFSFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITE 550
           K FSF EL +ATNG +N+LGRGAFG VY+GVL L  +EVE+AVK+LEK+IEQGEKEF+TE
Sbjct: 502 KAFSFQELLQATNGLRNKLGRGAFGTVYSGVLTLEAEEVEIAVKKLEKVIEQGEKEFLTE 561

Query: 551 VQVIGLTHHKNLVRLFCFCNEE-------------------------------------- 610
           VQVIGLTHHKNLVRL  FCNE+                                      
Sbjct: 562 VQVIGLTHHKNLVRLVGFCNEKNHRLLVYELMKNGTLSDFLFGEERRPSWDQRAETVYGI 621

Query: 611 ------------------------------------------------------------ 625
                                                                       
Sbjct: 622 ARGLLYLHEECEKQIIHCDIKPQNVLLDKNYTAKIADFGLAKLLNKDQTRTSTKVRGTMG 681

BLAST of HG10012672 vs. ExPASy TrEMBL
Match: A0A2K1Y1P6 (Receptor-like serine/threonine-protein kinase OS=Populus trichocarpa OX=3694 GN=POPTR_013G059900 PE=3 SV=2)

HSP 1 Score: 641.0 bits (1652), Expect = 5.3e-180
Identity = 356/789 (45.12%), Postives = 457/789 (57.92%), Query Frame = 0

Query: 11  LPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGRYLVGIVF 70
           LP  L F    AQ Q   NTI LG+SI AG+N  W+S SGDFAFGF+ +++G +LVGI F
Sbjct: 22  LPFVLSFLCSLAQPQITTNTINLGASITAGTNSSWRSPSGDFAFGFYPLLNGMFLVGIWF 81

Query: 71  DKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYNGT-TTSSAFMSNNG 130
           DK+PERTL WSANRDDPA+  STI     GQ VL H+N T   IYNGT   SSA M N+G
Sbjct: 82  DKIPERTLVWSANRDDPARTGSTINFTLDGQLVLTHSNGTGYLIYNGTFGASSALMQNDG 141

Query: 131 NFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGN 190
           NF++  +SS  IWQSFD PT+T+L GQ L +G++L+SNANGTVDYSTG++MLE+Q  DGN
Sbjct: 142 NFVVKTNSSEVIWQSFDSPTNTILLGQVLVMGKKLYSNANGTVDYSTGQYMLELQ-MDGN 201

Query: 191 VIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDY 250
           V+MSA++FADP Y FT T  N+N +++FNQ+TAF+YVVN T+I Y MTS  Q+  P+ DY
Sbjct: 202 VVMSAYKFADPGYWFTLTEGNQNVSLIFNQSTAFMYVVNHTSITYRMTS--QVPTPIGDY 261

Query: 251 YHRATVDDQGNFQQLIRIK-NGSGGEWRSVWK---FVENPCMVSNICGVFGFCTTIDNQK 310
           YHRAT++D GN QQ +  K NGSG  W  VW+       PC+  NICGV+GFCT+IDN  
Sbjct: 262 YHRATINDHGNLQQFVYHKENGSG--WTVVWEPESIKAEPCIPFNICGVYGFCTSIDNTT 321

Query: 311 VNCECLEGYSPIDPNIPSKGCYPNKIMDFC----SDSNFKMVKLENVDFPFLKDNSDATM 370
           +NC+CL GYSP DP+IPSKGCYP+ ++DFC    S SNF + +++N DFP   + + A M
Sbjct: 322 INCDCLPGYSPWDPSIPSKGCYPDTVIDFCAPNSSASNFTLEEIDNADFP---NGAFADM 381

Query: 371 VRAT--DANECEEVLRNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTI 430
            R T  D  EC + + ++CF  A V  +  CYKKR PLLNAR +IP+TNN VAF+K+P  
Sbjct: 382 ARVTPADVEECRKAIMDDCFAVAGVLVESVCYKKRTPLLNARRSIPSTNNIVAFIKIPKA 441

Query: 431 NN---VYSK--KSPSKEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPKPLD 490
           NN   +  K   SPS  ALLA  ++CS   ++F  ++IY+HP+ + +  +K+   PKP++
Sbjct: 442 NNNNQIQDKDDDSPSWIALLAGLLLCSIMTLLFATISIYHHPLAQPYISKKQLPVPKPVE 501

Query: 491 VNLKVFSFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEF 550
           +NLK FSF EL +ATNG +N+LGRGAFG VY+GVL L  +EVE+AVK+LEK+IEQGEKEF
Sbjct: 502 INLKAFSFQELLQATNGLRNKLGRGAFGTVYSGVLTLEAEEVEIAVKKLEKVIEQGEKEF 561

Query: 551 ITEVQVIGLTHHKNLVRLFCFCNEE----------------------------------- 610
           +TEVQVIGLTHHKNLVRL  FCNE+                                   
Sbjct: 562 LTEVQVIGLTHHKNLVRLVGFCNEKNHRLLVYELVKNGTLSDFLFGEERRPSWDQRAETV 621

Query: 611 ------------------------------------------------------------ 624
                                                                       
Sbjct: 622 YGIARGLLYLHEECETQIIHCDIKPQNVLLDKNYTAKIADFGLAKLLKKDQTRTSTKVRG 681

BLAST of HG10012672 vs. TAIR 10
Match: AT5G60900.1 (receptor-like protein kinase 1 )

HSP 1 Score: 179.9 bits (455), Expect = 6.5e-45
Identity = 167/602 (27.74%), Postives = 254/602 (42.19%), Query Frame = 0

Query: 6   LLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNE----FWQSSSGDFAFGFHQIV- 65
           ++HL L L L  F  ++Q    N ++ +G S+ A  ++     W+S SGDFAFGF +I  
Sbjct: 8   IIHLVLILQLQTFFVFSQ-NIRNGSVPVGESLTASESQQISSSWRSPSGDFAFGFRKIQP 67

Query: 66  -DGRYLVGIVFDKVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQV------- 125
            DG + + I FDK+ ++T+ W A        N+T  L P G  V + A+   V       
Sbjct: 68  NDG-FTLSIWFDKISDKTIVWHAQ-----AVNTTTGLVPNGSKVTLTADGGLVIADPRGQ 127

Query: 126 ---SIYNGTTTSSAFMSNNGNFMLLNSSSNP----IWQSFDHPTDTLLPGQALRIGQRLF 185
                 +G + S    +++GNF+L    S      +W SF++PTDTLLP Q + +G+ L 
Sbjct: 128 ELWRALSGGSVSRGRFTDDGNFVLFRDGSEDSDEVLWSSFENPTDTLLPNQNIEVGRNLS 187

Query: 186 SNANGTVDYSTGRFMLEVQGFDGNVIMSAFR---------FADPAYKFTGTVDNKNATIV 245
           S    T  +  GRF L ++  DGN+ + +           ++      T   +N    +V
Sbjct: 188 SRRTET-SFKKGRFSLRLED-DGNLQLHSLNAETASESDIYSQYYESNTNDPNNPGIQLV 247

Query: 246 FNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWR 305
           FNQ+     +    +           ++    Y      D  GN                
Sbjct: 248 FNQSGEIYVLQRNNSRFVVKDRDPDFSIAAPFYISTGPDDALGNM--------------- 307

Query: 306 SVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPIDPNIPSKGCYPNKIMDFCS 365
                          CG    C+  +N++  CEC E +   DP+     C P+  M  C 
Sbjct: 308 --------------ACGYNNICSLGNNKRPKCECPERFVLKDPSNEYGDCLPDFEMQTCR 367

Query: 366 DSN---------FKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVY-- 425
             N         ++ + LE  ++PF     D       D   C+    ++C   A ++  
Sbjct: 368 PENQTANSDVNLYEFITLEKTNWPF----GDYESYANYDEERCKASCLSDCLCAAVIFGT 427

Query: 426 -FDGACYKKRMPLLNARGNIPNTNNHVAFLKVPTINNVYSKKSPSKEALLAIFVVCSTFA 485
             D  C+KK+ PL                         + ++SP  ++   I V   + A
Sbjct: 428 NRDLKCWKKKFPL------------------------SHGERSPRGDSDTFIKVRNRSIA 487

Query: 486 VVFIAMAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGRV 545
            V         P+T          + K LD    VF++ EL EAT  F  +LGRGAFG V
Sbjct: 488 DV---------PVT--------GNRAKKLD---WVFTYGELAEATRDFTEELGRGAFGIV 523

Query: 546 YNGVL-MLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNEERLRD 566
           Y G L +    EV VAVK+L+++    EKEF  EV+VIG  HHKNLVRL  FCNE + + 
Sbjct: 548 YKGYLEVAGGSEVTVAVKKLDRLDLDNEKEFKNEVKVIGQIHHKNLVRLIGFCNEGQSQM 523


HSP 2 Score: 58.5 bits (140), Expect = 2.2e-08
Identity = 28/63 (44.44%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 554 CFCNEERLRDIISHDSEAMNEYGRFERITMVGLWCICPNPALRPSMKEVLLMLEGSIEVR 613
           CF  + RL D+   DSEAMN+    ER   + +WCI     +RP+M+ V  MLEG I+V 
Sbjct: 664 CF-RQGRLEDLTEDDSEAMNDMETVERYVKIAIWCIQEEHGMRPNMRNVTQMLEGVIQVF 723

Query: 614 IPP 617
            PP
Sbjct: 724 DPP 725

BLAST of HG10012672 vs. TAIR 10
Match: AT2G19130.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-37
Identity = 154/589 (26.15%), Postives = 258/589 (43.80%), Query Frame = 0

Query: 4   IELLHLSLPLFLCFFHCYAQTQYPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQI-VDG 63
           +  L L+   F+CFF          +TI  G   ++G      SS G +  GF +     
Sbjct: 2   VSFLTLTSFFFICFF---IHGSSAVDTIS-GDFTLSGDQTI-VSSDGTYEMGFFKPGSSS 61

Query: 64  RYLVGIVFDKVPERTLAWSANRDDP-AKANSTIILKPTGQFVLIHAN------NTQVSIY 123
            + +G+ + ++  +T+ W ANRD   +  NS++     G  +L+  N      +T ++  
Sbjct: 62  NFYIGMWYKQL-SQTILWVANRDKAVSDKNSSVFKISNGNLILLDGNYQTPVWSTGLNST 121

Query: 124 NGTTTSSAFMSNNGNFMLLNS----SSNPIWQSFDHPTDTLLPGQALRIGQRL-----FS 183
           +  +   A + ++GN +L       S+N +WQSFDHP DT LPG  +R+ +R       +
Sbjct: 122 SSVSALEAVLQDDGNLVLRTGGSSLSANVLWQSFDHPGDTWLPGVKIRLDKRTGKSQRLT 181

Query: 184 NANGTVDYSTGRFMLEVQG-------FDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQ 243
           +     D S G F LE+         ++G+    +    +P  +   +V       ++N 
Sbjct: 182 SWKSLEDPSPGLFSLELDESTAYKILWNGSNEYWSSGPWNPQSRIFDSVPEMRLNYIYN- 241

Query: 244 TTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVW 303
              F +  N T   +  +   QL V       R  +D  G  +Q   ++      W   W
Sbjct: 242 ---FSFFSNTTDSYFTYSIYNQLNV------SRFVMDVSGQIKQFTWLEGNKA--WNLFW 301

Query: 304 KFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPI-----DPNIPSKGCYPNKIMDF 363
                 C V   CG FG C+  D  +  C C +G+ P+     D    S GC     +  
Sbjct: 302 SQPRQQCQVYRYCGSFGICS--DKSEPFCRCPQGFRPMSQKDWDLKDYSAGCVRKTELQC 361

Query: 364 CSDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNECFRTAAVYFDGA--CYK 423
                 +  +L N+    L DNS+  ++  T  + C    + +C   A  Y +G+  C  
Sbjct: 362 SRGDINQFFRLPNMK---LADNSE--VLTRTSLSICASACQGDCSCKAYAYDEGSSKCLV 421

Query: 424 KRMPLLNARG-NIPNTNNHVAFLKVPT--INNVYSKKSPSKEALLAIFVVCSTFAVVFIA 483
               +LN +     N+  ++ +L++    + NV +    + + L+   V+ S   +V + 
Sbjct: 422 WSKDVLNLQQLEDENSEGNIFYLRLAASDVPNVGASGKSNNKGLIFGAVLGSLGVIVLVL 481

Query: 484 MAIYYHPITKGFFQRKKPTKPKPLDVNLKVFSFNELKEATNGFKNQLGRGAFGRVYNGVL 543
           + +    I    ++R+K  + +  D  L  FS+ EL+ AT  F ++LG G FG V+ G L
Sbjct: 482 LVV----ILILRYRRRKRMRGEKGDGTLSAFSYRELQNATKNFSDKLGGGGFGSVFKGAL 541

Query: 544 MLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNE 559
                  ++AVK+LE  I QGEK+F TEV  IG   H NLVRL  FC+E
Sbjct: 542 ---PDSSDIAVKRLEG-ISQGEKQFRTEVVTIGTIQHVNLVRLRGFCSE 557

BLAST of HG10012672 vs. TAIR 10
Match: AT4G00340.1 (receptor-like protein kinase 4 )

HSP 1 Score: 155.6 bits (392), Expect = 1.3e-37
Identity = 157/565 (27.79%), Postives = 245/565 (43.36%), Query Frame = 0

Query: 35  SSIIAGSNEFWQSSSGDFAFGFHQIVDG--RYLVGIVFDKVPERTLAWSANRDDPAK--A 94
           S +I   N+   S    F  GF    +G   + +GI +  +P  T  W ANR  P     
Sbjct: 23  SKVIIKGNQTILSFKAIFRLGFFSTTNGSSNWYLGISYASMPTPTHVWVANRIRPVSDPD 82

Query: 95  NSTIILKPTGQFVLIHANNTQVSIYNGTTTSSAF-MSNNGNFMLLNSSSNPIWQSFDHPT 154
           +ST+ L  TG  ++ +  +  V   +     + F  S  GN +L+N   +P+WQSFD+PT
Sbjct: 83  SSTLELTSTGYLIVSNLRDGVVWQTDNKQPGTDFRFSETGNLILINDDGSPVWQSFDNPT 142

Query: 155 DTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYKFTGTVD 214
           DT LPG  +  G    ++     D S G + L +     N     ++   P Y  TG   
Sbjct: 143 DTWLPGMNV-TGLTAMTSWRSLFDPSPGFYSLRLSP-SFNEFQLVYKGTTP-YWSTGNWT 202

Query: 215 NKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKN 274
            +    V   T  ++Y  +    + P T       P   +++     D  +  +L R   
Sbjct: 203 GEAFVGVPEMTIPYIYRFH---FVNPYT-------PTASFWYIVPPLDSVSEPRLTRFMV 262

Query: 275 GSGGE------------WRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGYSPI 334
           G+ G+            W   W   E+PC V N+CG  GFC++       C C+ G+ P 
Sbjct: 263 GANGQLKQYTWDPQTQSWNMFWLQPEDPCRVYNLCGQLGFCSS--ELLKPCACIRGFRPR 322

Query: 335 DPNIPSKGCYPNKIMDFCSDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVLRNE 394
           +        Y +       DS  K    E V    L+ + D  M R        +V ++ 
Sbjct: 323 NDAAWRSDDYSDGCRRENGDSGEKSDTFEAVG--DLRYDGDVKMSRL-------QVSKSS 382

Query: 395 CFRTAAVYFD--GACYKKRMP----LLNARGNIPNTNN------HVAFLKVPTINNVYSK 454
           C +T        G  +K++      LL +  N+ N+++       V +++ P   N  SK
Sbjct: 383 CAKTCLGNSSCVGFYHKEKSNLCKILLESPNNLKNSSSWTGVSEDVLYIREPKKGN--SK 442

Query: 455 KSPSKEALLAIFVVCSTF-AVVFIAMAIYYHPITKGFFQRKKPTKPKPLD----VNLKVF 514
            + SK    +I ++CS   ++  +   +    I     +++K T+ +  D    +NLKVF
Sbjct: 443 GNISK----SIIILCSVVGSISVLGFTLLVPLILLKRSRKRKKTRKQDEDGFAVLNLKVF 502

Query: 515 SFNELKEATNGFKNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQV 566
           SF EL+ ATNGF +++G G FG V+ G   L      VAVK+LE+    GE EF  EV  
Sbjct: 503 SFKELQSATNGFSDKVGHGGFGAVFKGT--LPGSSTFVAVKRLERP-GSGESEFRAEVCT 554


HSP 2 Score: 49.3 bits (116), Expect = 1.3e-05
Identity = 27/65 (41.54%), Postives = 35/65 (53.85%), Query Frame = 0

Query: 568 DSEAMNEYGRFE--RITMVGLWCICPNPALRPSMKEVLLMLEGSIEVRIPP-------LV 624
           DS    EY   E  R+  V +WCI  N  +RP+M  V+ MLEG +EV +PP       LV
Sbjct: 719 DSRLNGEYNTEEVTRMATVAIWCIQDNEEIRPAMGTVVKMLEGVVEVTVPPPPKLIQALV 778

BLAST of HG10012672 vs. TAIR 10
Match: AT4G11900.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 8.2e-32
Identity = 144/563 (25.58%), Postives = 235/563 (41.74%), Query Frame = 0

Query: 64  YLVGIVFDKVPERTLAWSANRDDPAKAN-STIILKPTGQFVLIHAN--NTQVSIYNGTTT 123
           Y +G+ +  V  +T+ W ANR+ P   + ST +LK     +++H N   T+ S   GT+ 
Sbjct: 66  YYIGMWYRHVSPQTIVWVANRESPLGGDASTYLLKILDGNLILHDNISATRKSHTEGTSR 125

Query: 124 SS-----------------------------AFMSNNGNFMLL---NSSSNPIWQSFDHP 183
            S                             A + ++GN +L    NSS+  +WQSFDHP
Sbjct: 126 RSPQKISEGNLLFHETVWSTGVNSSMSKDVQAVLFDSGNLVLRDGPNSSAAVLWQSFDHP 185

Query: 184 TDTLLPGQALRIGQRLFSNANGTVDYSTGRFMLEVQGFDGNVIMSAFRFADPAYKFTGTV 243
           +DT LPG  +R+G +LF++    +D S GR+ LE                DP      TV
Sbjct: 186 SDTWLPGGKIRLGSQLFTSWESLIDPSPGRYSLEF---------------DPKLHSLVTV 245

Query: 244 DNKNATIVFNQTTAFLYVVNGTTIIYPMTSQAQLTVPVNDYYHRATVDDQGNF------- 303
            N++ +  ++    + ++ +         ++   T+ +++ Y   +VD Q  +       
Sbjct: 246 WNRSKS-YWSSGPLYDWLQSFKGFPELQGTKLSFTLNMDESYITFSVDPQSRYRLVMGVS 305

Query: 304 -QQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVFGFCTTIDNQKVNCECLEGY------ 363
            Q ++++ +     WR +    +N C V N CG FG C   + +   C C+ G+      
Sbjct: 306 GQFMLQVWHVDLQSWRVILSQPDNRCDVYNSCGSFGICNE-NREPPPCRCVPGFKREFSQ 365

Query: 364 SPIDPNIPSKGCYPNKIMDFCSDSNFKMVKLENVDFPFLKDNSDATMVRATDANECEEVL 423
              D N  S GC     +  C   N + + +EN+      D + A+++ +     C    
Sbjct: 366 GSDDSNDYSGGCKRETYL-HCYKRNDEFLPIENM--KLATDPTTASVLTSGTFRTCASRC 425

Query: 424 RNECFRTAAVYFDGACYKKRMPLLNARGNIPNTNNHVAFLK-----VPTINNVYSKKSPS 483
             +C   A       C        N +  +     H  FL+     + T NN  ++ S  
Sbjct: 426 VADCSCQAYANDGNKCLVWTKDAFNLQ-QLDANKGHTFFLRLASSNISTANNRKTEHSKG 485

Query: 484 KEALLAIFVVCSTFAVVFIAMAIYYHPITKGFFQRKKPTKPK------------PLDVNL 543
           K  +L + V+ S  A     + +Y   I+    ++KK    K                N+
Sbjct: 486 KSIVLPL-VLASLVATAACFVGLYC-CISSRIRRKKKQRDEKHSRELLEGGLIDDAGENM 545

Query: 544 KVFSFNELKEATNGF--KNQLGRGAFGRVYNGVLMLNDQEVEVAVKQLEKMIEQGEKEFI 559
              + +++  ATN F  K +LG G FG VY G L      +EVA+K+L K   QG  EF 
Sbjct: 546 CYLNLHDIMVATNSFSRKKKLGEGGFGPVYKGKL---PNGMEVAIKRLSKKSSQGLTEFK 602

BLAST of HG10012672 vs. TAIR 10
Match: AT5G35370.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 9.1e-31
Identity = 144/596 (24.16%), Postives = 244/596 (40.94%), Query Frame = 0

Query: 6   LLHLSLPLFLCFFHCYAQTQ--YPNNTIKLGSSIIAGSNEFWQSSSGDFAFGFHQIVDGR 65
           LL LSL L   F  C +  +  YPN T      + +    F  S +  F  G        
Sbjct: 9   LLLLSLNLLFVFVSCASSIEFVYPNFTASNLRFVDSSKGAFLLSRNSIFKAGLFSPGGDD 68

Query: 66  YLVGIVFD--KVPERTLAWSANRDDPAKANSTIILKPTGQFVLIHANNTQVSIYN----G 125
              G  F    V   +  WS+NRD P  ++ T+ L P G   +I    +Q+ +++     
Sbjct: 69  SSTGFYFSVVHVDSGSTIWSSNRDSPVSSSGTMNLTPQG-ISVIEDGKSQIPVWSTPVLA 128

Query: 126 TTTSSAFMSNNGNFMLLNSSSNPIWQSFDHPTDTLLPGQALRIGQRLFSNANGTVDYSTG 185
           +   S  +++ GN +LL+  +  +W+SFD PTD+++ GQ L++G  L S +    D+STG
Sbjct: 129 SPVKSLRLTDAGNLLLLDHLNVSLWESFDFPTDSIVLGQRLKLGMFL-SGSVSRSDFSTG 188

Query: 186 RFMLEVQGFDGNVIMSAFRFADPAYKFTGTVDNKNATIVFNQTTAFLYVV--NGTTIIYP 245
            +   V   DG +      +          VD+         TT+ L ++  NGT ++  
Sbjct: 189 DYKFLVGESDGLMQWRGQNYWKLRMHIRANVDSNFPVEYLTVTTSGLALMARNGTVVV-- 248

Query: 246 MTSQAQLTVPVNDYYHRATVDDQGNFQQLIRIKNGSGGEWRSVWKFVENPCMVSNICGVF 305
                ++ +P +  +  A +D  G F     +   SG    + +    + C +  +CG  
Sbjct: 249 ----VRVALPPSSDFRVAKMDSSGKF----IVSRFSGKNLVTEFSGPMDSCQIPFVCGKL 308

Query: 306 GFCTTIDNQKVNCECLEGYSPIDPNIPS-KG-CYP----NKIMDFCSDSNFKMVKLENVD 365
           G C  +DN   N  C     P +  + + KG C P      +   C   N   ++L  + 
Sbjct: 309 GLC-NLDNASENQSC---SCPDEMRMDAGKGVCVPVSQSLSLPVSCEARNISYLEL-GLG 368

Query: 366 FPFLKDNSDATMVRATDANECEEVLRNECFRTAAVY--FDGACY--KKRMPLLNARGNIP 425
             +   +    +        C ++    C      Y     +CY  K     L+   N P
Sbjct: 369 VSYFSTHFTDPVEHGLPLLACHDICSKNCSCLGVFYENTSRSCYLVKDSFGSLSLVKNSP 428

Query: 426 NTNNHVAFLKV--------PTINNVYSKKSPSKEALLAIFVVCSTFAVVFIAMAIYYH-- 485
             ++ + ++K+        P  NN  ++   S   +  + + CS F ++     +++   
Sbjct: 429 ENHDLIGYVKLSIRKTNAQPPGNN--NRGGSSFPVIALVLLPCSGFFLLIALGLLWWRRC 488

Query: 486 -PITKGFFQRKKPTKPKPLDVN----------LKVFSFNELKEATNGFKNQLGRGAFGRV 545
             +     + K+ T+P   +             + F F EL++AT  FK Q+G G FG V
Sbjct: 489 AVMRYSSIREKQVTRPGSFESGDLGSFHIPGLPQKFEFEELEQATENFKMQIGSGGFGSV 548

Query: 546 YNGVLMLNDQEVEVAVKQLEKMIEQGEKEFITEVQVIGLTHHKNLVRLFCFCNEER 561
           Y G L     E  +AVK++      G +EF TE+ +IG   H NLV+L  FC   R
Sbjct: 549 YKGTL---PDETLIAVKKITNHGLHGRQEFCTEIAIIGNIRHTNLVKLRGFCARGR 582

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023527957.13.1e-21452.36G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 [Cucurbita ... [more]
XP_022137776.14.6e-21059.85G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 [Momordica ... [more]
KAF3953154.18.7e-19351.60hypothetical protein CMV_021374 [Castanea mollissima][more]
KAF3957004.11.7e-18853.82hypothetical protein CMV_017943 [Castanea mollissima][more]
KAF3947158.14.2e-18751.74hypothetical protein CMV_026671 [Castanea mollissima][more]
Match NameE-valueIdentityDescription
Q25AG33.3e-6234.10G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sa... [more]
Q0JEU64.3e-6234.10G-type lectin S-receptor-like serine/threonine-protein kinase LECRK3 OS=Oryza sa... [more]
Q25AG23.7e-6133.83G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sa... [more]
Q7FAZ03.7e-6133.83G-type lectin S-receptor-like serine/threonine-protein kinase LECRK4 OS=Oryza sa... [more]
Q7FAZ21.1e-6033.99G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 OS=Oryza sa... [more]
Match NameE-valueIdentityDescription
A0A6J1C7P52.2e-21059.85G-type lectin S-receptor-like serine/threonine-protein kinase LECRK2 OS=Momordic... [more]
A0A2C9WA553.2e-18546.54Receptor-like serine/threonine-protein kinase OS=Manihot esculenta OX=3983 GN=MA... [more]
A0A5J5C0459.4e-18547.58Receptor-like serine/threonine-protein kinase OS=Nyssa sinensis OX=561372 GN=F05... [more]
A0A6M2F7D42.0e-18245.36Receptor-like serine/threonine-protein kinase OS=Populus davidiana OX=266767 PE=... [more]
A0A2K1Y1P65.3e-18045.12Receptor-like serine/threonine-protein kinase OS=Populus trichocarpa OX=3694 GN=... [more]
Match NameE-valueIdentityDescription
AT5G60900.16.5e-4527.74receptor-like protein kinase 1 [more]
AT2G19130.11.3e-3726.15S-locus lectin protein kinase family protein [more]
AT4G00340.11.3e-3727.79receptor-like protein kinase 4 [more]
AT4G11900.18.2e-3225.58S-locus lectin protein kinase family protein [more]
AT5G35370.19.1e-3124.16S-locus lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 513..533
NoneNo IPR availableGENE3D2.90.10.30coord: 148..283
e-value: 1.4E-7
score: 33.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 462..565
e-value: 1.5E-23
score: 84.9
NoneNo IPR availablePANTHERPTHR47976:SF62SERINE/THREONINE-PROTEIN KINASEcoord: 3..560
coord: 559..619
NoneNo IPR availablePANTHERPTHR47976G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5coord: 3..560
coord: 559..619
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 36..148
e-value: 4.8E-11
score: 52.7
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 77..161
e-value: 8.9E-16
score: 58.3
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 22..146
score: 9.716936
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 492..561
e-value: 3.0E-11
score: 43.1
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 23..146
e-value: 3.4E-11
score: 45.2
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 73..150
IPR000858S-locus glycoprotein domainPFAMPF00954S_locus_glycopcoord: 243..316
e-value: 2.1E-9
score: 37.9
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 496..521
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 490..626
score: 9.68675
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 477..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012672.1HG10012672.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity