HG10012609 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012609
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionvesicle-associated protein 1-2-like
LocationChr01: 22752542 .. 22756996 (-)
RNA-Seq ExpressionHG10012609
SyntenyHG10012609
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTATGGAGCTTTTCGAAATTCAACCGGCCGAACTTAAGTTTACTTGTAAGTATAAGAGGAAACAGTTTTCTTTTTTTACATTTTGGTTCCAATGCATGATGTACATCCTATTATATATTATTAGGCAAGATGGCAGTGAGACCGCTGCAGAATTTAGCTTGCACGGTCTCGAATTGTTTTAGATGAAACTGAGGCTTAAATCACGAGATAAAAAGTTGATAGGCCTAGTTTAGGCTGTGAGTTGTAGCTTACAGGTGCTAGCTTACAATAAGAAGTTAGTAAACCAAATCTTTTGAATTATTTAAACCCCGTTTGATGACTATTTGGTTTTTTGTTTTTGAAAATTGTGCTTGTTTTCTCACCATTTCTCACCATGATTTTCATGTTCTTAAGGAAACATTTAAATTCTTGGACAAATTCAAAAAAACTACTTTTTTTTACCATTCAAAACTTGGTTTGGATTTTGAAAACATTTGGAAAAAAATAGACAAACAACAAAATAAAGAAACTTATAGGTGAAAGTAGTGTTTATAAACTTAATTTTCAAAAAATTACAAGTGAAAATCAAATGGTTATCAAACTGGACAGTAGCTAGTTGGAATTTGTTTCATGACCTGCAAAGTTTAAATGTTCGTTAAGATTTACTTGCTAGTTACCTTTTTTTTTATTTTTTAAAAGTACCCCAATGTACCTACCTGTTTATATCTGCACGTATGTTCTGCAAAGAAAAAGAAGATAGAAGAATGTTCTAGTCTATGTACTCATTGCCTGCAGTGTTATTTTAACAAGTTTTGTTTGCTTTCTTTCTCCAGTCGAGTTGAAGAAGCAAAGTTCATGCTTGATACAACTTATCAATAAATCCGAGCAACATGTTGCTTTCAAGGTGAAGTATTCAAAATGGATCGCACCAAATCTTTTTCCTGCCAATACCATTCCTTTTCAATGTTGTCTTATAATTTTAATTAGTAAAATTAGATTCTTTTTTTTTTTTGTTTTCCCCAATAGGTTCCAAAATTGAAAAGAATCTTAAAGCCTTAACTTTGTTCGCAGGTAAAAACTACATCTCCCAAGAAGTACTGTGTGCGACCCAATACTGGCATCATTAAGCCCAAGGATACGTGTGACTTCACAGGTATCATTAATGATGACTTCACAGGCATCATTAAGCCTGTCATTCTTTTAAAATGTTGGTGGTATTAACATGGAGTTGGCTGTTATATATCATATAACTATCTACTGATTTCTTCACACGAAACTGCCAATCTGTCTTAAACCATTTCAAAGATGTAATAAGATGGACATTTACATGCTACTTCAAAAGGCTTTGGAAAGTTTCTTTTTAATAATTATTTATTTATTTTGTGTATCTGTTCTCTGATTCCAACTCTGTCACTCTTGGATCTATTCAGTCACCATGCTAGCTCAGCGTACGGCTCCACCTGATATGCAATGCAAAGACAAGTTCCTGGTCCAAGGCACAGTCATCTCCCCTGGAACATCTGAAGAGGACATAACATCTGACGTGGTAAGAATTTGTAGTTGTTGCTTGCTTTACAGGCCTTTTTCTAAGATTTTTGTTATGCAAGGCAGTTTGCAAAAGATAGTGGCAAGCACATTGAAGAGAAGAAGCTGAAGGTTTTTCTAGTGAGCGCAACACCACCTCCTGTTTTGTTACCAATTAATGGTGAATTGAAACTAGATTCAAATCATGAAACTTCCATGCAAAGAGATAGAATGCAGACAGGAGTTGAGAACATACCTCCACCTCACAAGGTTTGACTAACTGTGCACTTTTCATGTGTGTATATTGTACTTCTGTTGTAAATCTATTCTTTACTTCTCTCTCCCTCCAGTCTTCACGTACTTCATTCTGATTGGAGGGAGGCTCTAGTTTCAAATTTTTGAGGATATTATTATATGCATATACGTAACATGGTTTTAAACCAAAGCATATTGGCTGTTTATTATTATTATAATACGTATTAGGTACCTTTACTTTTTATGGAAAAACATGATTGGATTGTGGTTGATTTTTCCCCCTGAATTTATCTCTTGATTGGAATTGATATATTCTAGGCTGCAGAAGACTCCAATGGACTTGATACCTGCAAACACATAGATGAACTTAGACCAGTTGATACCCCAGAATTGTTATCTCCACCTTATAAGGTATGACCATGCAAGTAACTCTGCCATGTCATATGTGCTTATATTATATACTTGTTAGTGTTGTCTTCTCCAGTCAATACACACACACACATGTATTCTTGTCAGTCCTTCTCTAGTTTTGAACTGTAAAGGGATTTCCCCTGGAGGTATATGTTCGTTTCCCATCATTTCATGTTTTGAATCCTCGAGTTTGTGGTCTTACTATTGCATGAAAAGCTACATGAGGTTTGAAATTCCTGCAGTGATGAGACACTTGGTTTCCATATAGTTTATCGATGTTCTCCTTCTTTCCAAATACGATTGACTTTTCTCCTTGTGTGTTTATGATGTTTACCAGGTGGCCGAGGGAGTTGAGAAAAGTGACACTTGTAAAGATTCAGATGAGAATAGAGCAGCTGAGGATGTTTCAACAAGACAAAATGAGGATGTGGTGGCCGAACCAATTGAGAATATTGAAACCAGGCCAGCTGAGGGAATTAAGGAATCAAAACTGGCGAAGGATCTACCAGAGTTAAACTTAACTAAAAACTTTCAAGAGCTGAAATCAAAGCTAACTCTCATGGATGCAGAACTACAGGAGGTAAGACTTTTTCTCTTGCAGTTAGATAATCATGCACTCCCTGTGGTATATATGTTTCTCCTTACAAACAAGAAAATTAACTTTGGCAACTGGCTAGTCACAGTCAATTGATTGGATAAGTTCATGGCCTGTGAGTAAAACTTTTGCCTCTGGTACATCTCCAGGTCTTTAAATGTATATTTGCTTATGTTGCTATATCTTTACTGTGATAAGTTCTTAATTTACGAAGGCAGAAAATAGTGATGGTAATTAAGGGGGTGTTTGGCAGGCCATTTGAAAACAAAAGATTCAATAAAAATAGAAATGTATTTTATCTTTTCAAACATGCGTTTGGTGGTAGATTCAAAAGTTGGATTCGAATTTAAACAATTAATGTTTAAAATGTGTTTGTTACCCACTAAATTAATATTAATTAATTTATGAACATGTTTTATGTTCAAAATTTTGTATAATTCTTATTTTGTAATATCATATAGTTTATAATACATTATACCATACATATTTTAGTTTAACAAAAATGAGTTGAGTTTATAACTTGAAATACTATATTTATTAATGTTTTTGGAATCTTACCAAACTGGTCATAAGTAATCATGATTCAACGATTTTGAGAATTCTGAGTAAGATTGTGAGGTTCCTTGATTGTCCCTTGATTTTTCAATGTGGGATCCTCAACATGCTGCTTCAAGATGGTGCCTCTTTGGGTTCACCATTCTTGGATCAGATCCTAATTTTTTTTTTTGTTGTTGGACCGAGTGCCCATTTGAGCTTCATGGATCTTATACTATATTAGATAACTTGGGTTCCATTTCAAAATCAATTGACAGTGAGAGAGTAGCCCAAATATCTTATAAACATTATGAGGTCTTTGTAATTTTTAATGTGGGATCCTCAACATATTTATAAAAGAAAAGAAACTAATAATTGCCTCCTCAGGAGCAAGCTTTGCAACCTGTTTCTTCCTTCCACATCTTCTATACGTACAACATTTTTTTTTCCTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGCAAGGATTTATAACGCTCCATTACAGCTTTCATGTTTGTTAGTTGTTGCTAGATATAACTAATTTTTCAGGTACTAATGAAGAATTTATGAACATATGCAGGCTGAAGCTACCATAATGAGGCTGAAAAAGGAGAGGATGATAACAACTCAAGAAAGGGAAATGCTCAAGCGTGACTTGGTAAATCTTTTTTTTCCTTTACACTTTTAAGCATAGATTTTAGTCATTGAACTCACATCAAACTCCATATTGAAGATGACAGTCTTGAATCTAGAGAAGACACCGCTTCATAATCTTTAAAATTTGTATAAACTCAAAGAGGCTGAATCAAAGTTTTGAAACTTTGGGAATGAAAGAAAAAACCCGTCGTCCCTTCGCCTTCTTGAAAATGATGAGGGATAGTTTATCGCCTGCTTTTGAAACGAGAAACCTATCAAATTGAGCCTTAACTAGTCACTATACACATTTATTCATGCTATGCTTGATCATACAGTATAGAGGCACCCTGATTTTTAGATCTTGGGGAGTTTGAGGGACATGATACTGATTTCTTGATGCAGGAGACATTGAGAAAAACTGGCCAAAGAAGTATTCAAGTAGGCTTCCCATTAATGTATGTTGTGATGGTTGCTTTTATCAGTCTTCTTGTTGGATACTTCATCGATCCCTAG

mRNA sequence

ATGACTATGGAGCTTTTCGAAATTCAACCGGCCGAACTTAAGTTTACTTTCGAGTTGAAGAAGCAAAGTTCATGCTTGATACAACTTATCAATAAATCCGAGCAACATGTTGCTTTCAAGGTAAAAACTACATCTCCCAAGAAGTACTGTGTGCGACCCAATACTGGCATCATTAAGCCCAAGGATACGTGTGACTTCACAGTCACCATGCTAGCTCAGCGTACGGCTCCACCTGATATGCAATGCAAAGACAAGTTCCTGGTCCAAGGCACAGTCATCTCCCCTGGAACATCTGAAGAGGACATAACATCTGACGTGTTTGCAAAAGATAGTGGCAAGCACATTGAAGAGAAGAAGCTGAAGGTTTTTCTAGTGAGCGCAACACCACCTCCTGTTTTGTTACCAATTAATGGTGAATTGAAACTAGATTCAAATCATGAAACTTCCATGCAAAGAGATAGAATGCAGACAGGAGTTGAGAACATACCTCCACCTCACAAGGCTGCAGAAGACTCCAATGGACTTGATACCTGCAAACACATAGATGAACTTAGACCAGTTGATACCCCAGAATTGTTATCTCCACCTTATAAGGTGGCCGAGGGAGTTGAGAAAAGTGACACTTGTAAAGATTCAGATGAGAATAGAGCAGCTGAGGATGTTTCAACAAGACAAAATGAGGATGTGGTGGCCGAACCAATTGAGAATATTGAAACCAGGCCAGCTGAGGGAATTAAGGAATCAAAACTGGCGAAGGATCTACCAGAGTTAAACTTAACTAAAAACTTTCAAGAGCTGAAATCAAAGCTAACTCTCATGGATGCAGAACTACAGGAGGCTGAAGCTACCATAATGAGGCTGAAAAAGGAGAGGATGATAACAACTCAAGAAAGGGAAATGCTCAAGCGTGACTTGGAGACATTGAGAAAAACTGGCCAAAGAAGTATTCAAGTAGGCTTCCCATTAATGTATGTTGTGATGGTTGCTTTTATCAGTCTTCTTGTTGGATACTTCATCGATCCCTAG

Coding sequence (CDS)

ATGACTATGGAGCTTTTCGAAATTCAACCGGCCGAACTTAAGTTTACTTTCGAGTTGAAGAAGCAAAGTTCATGCTTGATACAACTTATCAATAAATCCGAGCAACATGTTGCTTTCAAGGTAAAAACTACATCTCCCAAGAAGTACTGTGTGCGACCCAATACTGGCATCATTAAGCCCAAGGATACGTGTGACTTCACAGTCACCATGCTAGCTCAGCGTACGGCTCCACCTGATATGCAATGCAAAGACAAGTTCCTGGTCCAAGGCACAGTCATCTCCCCTGGAACATCTGAAGAGGACATAACATCTGACGTGTTTGCAAAAGATAGTGGCAAGCACATTGAAGAGAAGAAGCTGAAGGTTTTTCTAGTGAGCGCAACACCACCTCCTGTTTTGTTACCAATTAATGGTGAATTGAAACTAGATTCAAATCATGAAACTTCCATGCAAAGAGATAGAATGCAGACAGGAGTTGAGAACATACCTCCACCTCACAAGGCTGCAGAAGACTCCAATGGACTTGATACCTGCAAACACATAGATGAACTTAGACCAGTTGATACCCCAGAATTGTTATCTCCACCTTATAAGGTGGCCGAGGGAGTTGAGAAAAGTGACACTTGTAAAGATTCAGATGAGAATAGAGCAGCTGAGGATGTTTCAACAAGACAAAATGAGGATGTGGTGGCCGAACCAATTGAGAATATTGAAACCAGGCCAGCTGAGGGAATTAAGGAATCAAAACTGGCGAAGGATCTACCAGAGTTAAACTTAACTAAAAACTTTCAAGAGCTGAAATCAAAGCTAACTCTCATGGATGCAGAACTACAGGAGGCTGAAGCTACCATAATGAGGCTGAAAAAGGAGAGGATGATAACAACTCAAGAAAGGGAAATGCTCAAGCGTGACTTGGAGACATTGAGAAAAACTGGCCAAAGAAGTATTCAAGTAGGCTTCCCATTAATGTATGTTGTGATGGTTGCTTTTATCAGTCTTCTTGTTGGATACTTCATCGATCCCTAG

Protein sequence

MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKPKDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKLKVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKHIDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETRPAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP
Homology
BLAST of HG10012609 vs. NCBI nr
Match: XP_038902396.1 (vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida] >XP_038902435.1 vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida] >XP_038902474.1 vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida] >XP_038902500.1 vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida] >XP_038902553.1 vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 317/341 (92.96%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVI+PGTSEEDITSDVFAKD+GKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVITPGTSEEDITSDVFAKDTGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATPPPVLLPINGELKLDSN+ETSM RDRMQTGVENIPPPH  AEDSNGL TC H
Sbjct: 121 KVFLVSATPPPVLLPINGELKLDSNNETSMPRDRMQTGVENIPPPHNVAEDSNGL-TCNH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVD P LLSPPYKVAEGVEK DTCKDSDENRAAEDVSTRQNEDVVA PIE+IET 
Sbjct: 181 IDELRPVDNPVLLSPPYKVAEGVEKIDTCKDSDENRAAEDVSTRQNEDVVAGPIEHIETT 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           PAEGI+ESKLAK LPELNLTK+FQELKSKLTLMDAEL EAEATIMRLKKERM+T++EREM
Sbjct: 241 PAEGIEESKLAKVLPELNLTKDFQELKSKLTLMDAELLEAEATIMRLKKERMVTSKEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYF+ P
Sbjct: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFVHP 340

BLAST of HG10012609 vs. NCBI nr
Match: XP_008438789.1 (PREDICTED: vesicle-associated protein 2-2-like isoform X1 [Cucumis melo])

HSP 1 Score: 591.7 bits (1524), Expect = 4.2e-165
Identity = 311/341 (91.20%), Postives = 320/341 (93.84%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLI LINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIQPAELKFTFELKKQSSCLIHLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATP PVL PINGELKLDSNHETSM RDRMQTGVENIPPP K AEDSNGLDT KH
Sbjct: 121 KVFLVSATPTPVLSPINGELKLDSNHETSMPRDRMQTGVENIPPPSKVAEDSNGLDTWKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVDTP  LSPPYKVAEGVEK DTCKD DEN AAE+V  RQNE  VAE +ENIET 
Sbjct: 181 IDELRPVDTPVSLSPPYKVAEGVEKIDTCKDVDENGAAENVQIRQNE--VAESVENIETM 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P+EGI+ESKLAKDLPELNLTK+FQELKSKLTLMDAEL EAEATIMRLKKERM+TTQEREM
Sbjct: 241 PSEGIEESKLAKDLPELNLTKDFQELKSKLTLMDAELLEAEATIMRLKKERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRK+GQRSIQVGFPLMYV+MVA ISLLVGYFI P
Sbjct: 301 LKRDLETLRKSGQRSIQVGFPLMYVLMVACISLLVGYFIHP 339

BLAST of HG10012609 vs. NCBI nr
Match: XP_004134182.1 (vesicle-associated protein 2-2 [Cucumis sativus] >XP_011651023.1 vesicle-associated protein 2-2 [Cucumis sativus] >XP_011651024.1 vesicle-associated protein 2-2 [Cucumis sativus] >XP_031739299.1 vesicle-associated protein 2-2 [Cucumis sativus] >KAE8650376.1 hypothetical protein Csa_009727 [Cucumis sativus])

HSP 1 Score: 587.4 bits (1513), Expect = 7.8e-164
Identity = 308/341 (90.32%), Postives = 321/341 (94.13%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           K+TCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL
Sbjct: 61  KNTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFL SATP PVLLPINGELKLDSNHETSM RDRMQTGVENIPPP K AEDSNGLDT KH
Sbjct: 121 KVFLASATPTPVLLPINGELKLDSNHETSMPRDRMQTGVENIPPPSKVAEDSNGLDTRKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVDTP  LSPPYKVAEGVEK DTCKD+DEN AAE+V TR++E  VAE +ENIET 
Sbjct: 181 IDELRPVDTPVSLSPPYKVAEGVEKIDTCKDADENGAAENVPTRRSE--VAESVENIETM 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           PAEGI+ESKL+KDLPELNLTK+FQELKSKL LMDAEL EAEATIMRLKKER +TTQEREM
Sbjct: 241 PAEGIEESKLSKDLPELNLTKDFQELKSKLALMDAELLEAEATIMRLKKERTVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRK+GQRSIQVGFPLMYV+MVA ISLLVGYFI P
Sbjct: 301 LKRDLETLRKSGQRSIQVGFPLMYVLMVACISLLVGYFIHP 339

BLAST of HG10012609 vs. NCBI nr
Match: XP_023539031.1 (vesicle-associated protein 1-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 568.5 bits (1464), Expect = 3.8e-158
Identity = 294/341 (86.22%), Postives = 313/341 (91.79%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEI+P ELKFTFELK QSSCLIQLINKS+QH+AFKVKTTSPKKYCVRPNTG+IKP
Sbjct: 1   MTMELFEIRPGELKFTFELKTQSSCLIQLINKSDQHIAFKVKTTSPKKYCVRPNTGVIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVI  GTSEEDIT D+FAK SGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVIPAGTSEEDITPDLFAKGSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATPPPVLLPINGELKLD N ETS+ +DRMQTGVENIPPP K AED+NGLDT KH
Sbjct: 121 KVFLVSATPPPVLLPINGELKLDPNLETSLPKDRMQTGVENIPPPLKVAEDANGLDTRKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVD+P  LSPPYKVA GVEK DTCK+SD+NR AEDV+TRQNED VAEPI+N+ET 
Sbjct: 181 IDELRPVDSPLQLSPPYKVAVGVEKLDTCKESDDNREAEDVATRQNEDAVAEPIDNVETT 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P EGI+ESK  KDLPELNLTK+  ELKSKLTLMDAEL EAEATIMRLK+ERM+TTQEREM
Sbjct: 241 PVEGIEESKPVKDLPELNLTKDLLELKSKLTLMDAELIEAEATIMRLKQERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRKTG RSIQVGFPLMYVVMVA IS+LVGYFI P
Sbjct: 301 LKRDLETLRKTGHRSIQVGFPLMYVVMVALISVLVGYFIHP 341

BLAST of HG10012609 vs. NCBI nr
Match: XP_022939166.1 (vesicle-associated protein 1-2-like [Cucurbita moschata] >XP_022939172.1 vesicle-associated protein 1-2-like [Cucurbita moschata])

HSP 1 Score: 568.5 bits (1464), Expect = 3.8e-158
Identity = 293/341 (85.92%), Postives = 312/341 (91.50%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEI+P ELKFTFELK QSSCLIQLINKS+QH+AFKVKTTSPKKYCVRPNTG+IKP
Sbjct: 1   MTMELFEIRPGELKFTFELKTQSSCLIQLINKSDQHIAFKVKTTSPKKYCVRPNTGVIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVI  GTSEEDIT D+FAK SGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVIPAGTSEEDITPDLFAKGSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVS TPPPVLLPINGELKLD N ETS+ +DRMQTGVENIPPP K AED+NGLDT KH
Sbjct: 121 KVFLVSTTPPPVLLPINGELKLDPNLETSLPKDRMQTGVENIPPPLKVAEDANGLDTRKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVD+P  LSPPYKVAEGVEK DTCKDSD+NR AED++TRQNED VAEPI N+ET 
Sbjct: 181 IDELRPVDSPVQLSPPYKVAEGVEKLDTCKDSDDNREAEDIATRQNEDAVAEPIGNVETT 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P EGI+ESK  KDLPELNLTK+  ELKSKLTLMDAEL EAEATIMRLK+ERM+TTQEREM
Sbjct: 241 PVEGIEESKPVKDLPELNLTKDLLELKSKLTLMDAELIEAEATIMRLKQERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRKTG RSIQVGFPLMYV+MVA IS+LVGYFI P
Sbjct: 301 LKRDLETLRKTGHRSIQVGFPLMYVLMVALISVLVGYFIHP 341

BLAST of HG10012609 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 149/386 (38.60%), Postives = 219/386 (56.74%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           M M L +IQP  L+F  +LKKQ+SC++QL N +  +VAFKVKTTSPKKYCVRPN G++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           K TC+FTV M A +  PPDM CKDKFL+Q T +S  T++EDIT+ +F+K  GKHIEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           +V LV  +  P L PIN   K  +  E S+ +DR+ +  E + PP    E         H
Sbjct: 121 RVTLVPPSDSPELSPINTP-KQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGH 180

Query: 181 IDELRPVD-TPELLSPPYKVAEGVEKSDTCKD-------SDENRAAEDVS---TRQNEDV 240
            DEL+  D   EL +P     + VE   T  D        D +R A++      R ++D 
Sbjct: 181 -DELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDA 240

Query: 241 -----------VAEPIENIETRPAE-----GI---KESKLAKD----------------L 300
                      +  P++     P +     GI    E K++K+                L
Sbjct: 241 DDGRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGL 300

Query: 301 PELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKRDLETLR-KTGQ 340
            EL L K+ +E+K K+  ++++L++A++TI +L +ER I++Q R+ L+ +L  LR K   
Sbjct: 301 DELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIV 360

BLAST of HG10012609 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.4e-37
Identity = 77/130 (59.23%), Postives = 101/130 (77.69%), Query Frame = 0

Query: 2   TMELFEIQPAELKFTFELKKQSSCLIQLINK-SEQHVAFKVKTTSPKKYCVRPNTGIIKP 61
           T +L  I P ELKF FELKKQSSC +QL NK + Q VAFKVKTT+P+KYCVRPNTG++ P
Sbjct: 3   TGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLP 62

Query: 62  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 121
            D+C+ TVTM AQ+ AP DMQCKDKFLVQ  V+S GT+ +++ +++F K++G+ IE+ KL
Sbjct: 63  GDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKL 122

Query: 122 KVFLVSATPP 131
           +V  + A PP
Sbjct: 123 RVVYIPANPP 132

BLAST of HG10012609 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.5e-36
Identity = 108/336 (32.14%), Postives = 155/336 (46.13%), Query Frame = 0

Query: 4   ELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKPKDT 63
           EL  ++P +L+F FELKKQ SC + L NK++ +VAFKVKTT+PKKYCVRPNTG++ P+ T
Sbjct: 21  ELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRST 80

Query: 64  CDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKLKVF 123
           C+  VTM AQ+ AP DMQCKDKFL+QG + SPG + +++T ++F+K++G  +EE KL+V 
Sbjct: 81  CEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVT 140

Query: 124 LVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKHIDE 183
            V+   PP                                P H+ +E+ +          
Sbjct: 141 YVAPPRPP-------------------------------SPVHEGSEEGS---------- 200

Query: 184 LRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETRPAE 243
                     SP   V            SD    +E    R   D  A   EN       
Sbjct: 201 ----------SPRASV------------SDNGHGSEFSFERFIVDNKAGHQEN------- 253

Query: 244 GIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKR 303
                                              EA A I +L +E+    Q    L+R
Sbjct: 261 ---------------------------------TSEARALITKLTEEKQSAIQLNNRLQR 253

Query: 304 DLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFI 340
           +L+ LR+  ++S   G P MYV++V  I L++GY +
Sbjct: 321 ELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIM 253

BLAST of HG10012609 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 8.5e-36
Identity = 71/131 (54.20%), Postives = 98/131 (74.81%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           M+ EL  I P +L+F FELKKQ SC + L NK++ +VAFKVKTT+PKKYCVRPNTG++ P
Sbjct: 1   MSNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           + + +  VTM AQ+ AP D+QCKDKFL+Q  V SPG + +D+T ++F+K++G  +EE KL
Sbjct: 61  RSSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKL 120

Query: 121 KVFLVSATPPP 132
           +V  V+   PP
Sbjct: 121 RVVYVAPPRPP 131


HSP 2 Score: 40.4 bits (93), Expect = 4.7e-02
Identity = 22/61 (36.07%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 279 EAEATIMRLKKERMITTQEREMLKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYF 338
           EA A + +L +E+    Q    L+++L+ LR+  +RS   G P MYV++V  I L++GY 
Sbjct: 176 EARALVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYI 235

Query: 339 I 340
           +
Sbjct: 236 M 236

BLAST of HG10012609 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 73/123 (59.35%), Postives = 93/123 (75.61%), Query Frame = 0

Query: 4   ELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKPKDT 63
           +L  IQP ELKF FEL+KQS C +++ NK+E +VAFKVKTTSPKKY VRPNTG+I+P D+
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 64  CDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKLKVF 123
           C   VT+ AQR  PPDMQCKDKFL+Q T++ P T  +++  D F KDSGK + E KLKV 
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 124 LVS 127
            ++
Sbjct: 128 YIT 130

BLAST of HG10012609 vs. ExPASy TrEMBL
Match: A0A1S3AXX0 (vesicle-associated protein 2-2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483790 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.0e-165
Identity = 311/341 (91.20%), Postives = 320/341 (93.84%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLI LINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIQPAELKFTFELKKQSSCLIHLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATP PVL PINGELKLDSNHETSM RDRMQTGVENIPPP K AEDSNGLDT KH
Sbjct: 121 KVFLVSATPTPVLSPINGELKLDSNHETSMPRDRMQTGVENIPPPSKVAEDSNGLDTWKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVDTP  LSPPYKVAEGVEK DTCKD DEN AAE+V  RQNE  VAE +ENIET 
Sbjct: 181 IDELRPVDTPVSLSPPYKVAEGVEKIDTCKDVDENGAAENVQIRQNE--VAESVENIETM 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P+EGI+ESKLAKDLPELNLTK+FQELKSKLTLMDAEL EAEATIMRLKKERM+TTQEREM
Sbjct: 241 PSEGIEESKLAKDLPELNLTKDFQELKSKLTLMDAELLEAEATIMRLKKERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRK+GQRSIQVGFPLMYV+MVA ISLLVGYFI P
Sbjct: 301 LKRDLETLRKSGQRSIQVGFPLMYVLMVACISLLVGYFIHP 339

BLAST of HG10012609 vs. ExPASy TrEMBL
Match: A0A0A0L7T6 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G151410 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.8e-164
Identity = 308/341 (90.32%), Postives = 321/341 (94.13%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 98  MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 157

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           K+TCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL
Sbjct: 158 KNTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 217

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFL SATP PVLLPINGELKLDSNHETSM RDRMQTGVENIPPP K AEDSNGLDT KH
Sbjct: 218 KVFLASATPTPVLLPINGELKLDSNHETSMPRDRMQTGVENIPPPSKVAEDSNGLDTRKH 277

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVDTP  LSPPYKVAEGVEK DTCKD+DEN AAE+V TR++E  VAE +ENIET 
Sbjct: 278 IDELRPVDTPVSLSPPYKVAEGVEKIDTCKDADENGAAENVPTRRSE--VAESVENIETM 337

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           PAEGI+ESKL+KDLPELNLTK+FQELKSKL LMDAEL EAEATIMRLKKER +TTQEREM
Sbjct: 338 PAEGIEESKLSKDLPELNLTKDFQELKSKLALMDAELLEAEATIMRLKKERTVTTQEREM 397

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRK+GQRSIQVGFPLMYV+MVA ISLLVGYFI P
Sbjct: 398 LKRDLETLRKSGQRSIQVGFPLMYVLMVACISLLVGYFIHP 436

BLAST of HG10012609 vs. ExPASy TrEMBL
Match: A0A6J1FF59 (vesicle-associated protein 1-2-like OS=Cucurbita moschata OX=3662 GN=LOC111445158 PE=4 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 1.8e-158
Identity = 293/341 (85.92%), Postives = 312/341 (91.50%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEI+P ELKFTFELK QSSCLIQLINKS+QH+AFKVKTTSPKKYCVRPNTG+IKP
Sbjct: 1   MTMELFEIRPGELKFTFELKTQSSCLIQLINKSDQHIAFKVKTTSPKKYCVRPNTGVIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVI  GTSEEDIT D+FAK SGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVIPAGTSEEDITPDLFAKGSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVS TPPPVLLPINGELKLD N ETS+ +DRMQTGVENIPPP K AED+NGLDT KH
Sbjct: 121 KVFLVSTTPPPVLLPINGELKLDPNLETSLPKDRMQTGVENIPPPLKVAEDANGLDTRKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVD+P  LSPPYKVAEGVEK DTCKDSD+NR AED++TRQNED VAEPI N+ET 
Sbjct: 181 IDELRPVDSPVQLSPPYKVAEGVEKLDTCKDSDDNREAEDIATRQNEDAVAEPIGNVETT 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P EGI+ESK  KDLPELNLTK+  ELKSKLTLMDAEL EAEATIMRLK+ERM+TTQEREM
Sbjct: 241 PVEGIEESKPVKDLPELNLTKDLLELKSKLTLMDAELIEAEATIMRLKQERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRKTG RSIQVGFPLMYV+MVA IS+LVGYFI P
Sbjct: 301 LKRDLETLRKTGHRSIQVGFPLMYVLMVALISVLVGYFIHP 341

BLAST of HG10012609 vs. ExPASy TrEMBL
Match: A0A6J1I9H3 (vesicle-associated protein 1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111472319 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.3e-158
Identity = 295/341 (86.51%), Postives = 311/341 (91.20%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEI+P ELK TFELK QSSCLIQLINKS+QH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIRPGELKLTFELKTQSSCLIQLINKSDQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVI  GTSEEDIT D+FAK SGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVIPAGTSEEDITPDLFAKGSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATPPPVLLPINGELKLD N ETS+ +DRMQTGVENIPPP K AED+NGLDT KH
Sbjct: 121 KVFLVSATPPPVLLPINGELKLDPNLETSLPKDRMQTGVENIPPPLKVAEDANGLDTRKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVD+P  LSPPYKVAEGVEK DTCKDSD+NR AEDV+TRQNED VAEPI N+ET 
Sbjct: 181 IDELRPVDSPLQLSPPYKVAEGVEKLDTCKDSDDNREAEDVATRQNEDAVAEPIGNVETT 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P EGI+ESK  KDLPELNLTK+  ELKSKLTLMDAEL EAEATIMRLK+ERM+TTQEREM
Sbjct: 241 PVEGIEESKPVKDLPELNLTKDLLELKSKLTLMDAELIEAEATIMRLKQERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLMYVVMVAFISLLVGYFIDP 342
           LKRDLETLRKTG RSIQVGFPLMYVV VA IS+LVGYFI P
Sbjct: 301 LKRDLETLRKTGHRSIQVGFPLMYVVTVALISVLVGYFIHP 341

BLAST of HG10012609 vs. ExPASy TrEMBL
Match: A0A5D3CXC6 (Vesicle-associated protein 2-2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00390 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.1e-158
Identity = 296/323 (91.64%), Postives = 304/323 (94.12%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           MTMELFEIQPAELKFTFELKKQSSCLI LINKSEQH+AFKVKTTSPKKYCVRPNTGIIKP
Sbjct: 1   MTMELFEIQPAELKFTFELKKQSSCLIHLINKSEQHIAFKVKTTSPKKYCVRPNTGIIKP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL
Sbjct: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           KVFLVSATP PVL PINGELKLDSNHETSM RDRMQTGVENIPPP K AEDSNGLDT KH
Sbjct: 121 KVFLVSATPTPVLSPINGELKLDSNHETSMPRDRMQTGVENIPPPSKVAEDSNGLDTWKH 180

Query: 181 IDELRPVDTPELLSPPYKVAEGVEKSDTCKDSDENRAAEDVSTRQNEDVVAEPIENIETR 240
           IDELRPVDTP  LSPPYKVAEGVEK DTCKD DEN AAE+V  RQNE  VAE +ENIET 
Sbjct: 181 IDELRPVDTPVSLSPPYKVAEGVEKIDTCKDVDENGAAENVQIRQNE--VAESVENIETM 240

Query: 241 PAEGIKESKLAKDLPELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREM 300
           P+EGI+ESKLAKDLPELNLTK+FQELKSKLTLMDAEL EAEATIMRLKKERM+TTQEREM
Sbjct: 241 PSEGIEESKLAKDLPELNLTKDFQELKSKLTLMDAELLEAEATIMRLKKERMVTTQEREM 300

Query: 301 LKRDLETLRKTGQRSIQVGFPLM 324
           LKRDLETLRK+GQRSIQVGFPLM
Sbjct: 301 LKRDLETLRKSGQRSIQVGFPLM 321

BLAST of HG10012609 vs. TAIR 10
Match: AT1G08820.1 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 221.9 bits (564), Expect = 8.1e-58
Identity = 149/386 (38.60%), Postives = 219/386 (56.74%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           M M L +IQP  L+F  +LKKQ+SC++QL N +  +VAFKVKTTSPKKYCVRPN G++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           K TC+FTV M A +  PPDM CKDKFL+Q T +S  T++EDIT+ +F+K  GKHIEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           +V LV  +  P L PIN   K  +  E S+ +DR+ +  E + PP    E         H
Sbjct: 121 RVTLVPPSDSPELSPINTP-KQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGH 180

Query: 181 IDELRPVD-TPELLSPPYKVAEGVEKSDTCKD-------SDENRAAEDVS---TRQNEDV 240
            DEL+  D   EL +P     + VE   T  D        D +R A++      R ++D 
Sbjct: 181 -DELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDA 240

Query: 241 -----------VAEPIENIETRPAE-----GI---KESKLAKD----------------L 300
                      +  P++     P +     GI    E K++K+                L
Sbjct: 241 DDGRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGL 300

Query: 301 PELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKRDLETLR-KTGQ 340
            EL L K+ +E+K K+  ++++L++A++TI +L +ER I++Q R+ L+ +L  LR K   
Sbjct: 301 DELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIV 360

BLAST of HG10012609 vs. TAIR 10
Match: AT1G08820.2 (vamp/synaptobrevin-associated protein 27-2 )

HSP 1 Score: 221.9 bits (564), Expect = 8.1e-58
Identity = 149/386 (38.60%), Postives = 219/386 (56.74%), Query Frame = 0

Query: 1   MTMELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKP 60
           M M L +IQP  L+F  +LKKQ+SC++QL N +  +VAFKVKTTSPKKYCVRPN G++ P
Sbjct: 1   MNMPLLDIQPRTLQFAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAP 60

Query: 61  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 120
           K TC+FTV M A +  PPDM CKDKFL+Q T +S  T++EDIT+ +F+K  GKHIEE KL
Sbjct: 61  KSTCEFTVIMQAFKEPPPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKL 120

Query: 121 KVFLVSATPPPVLLPINGELKLDSNHETSMQRDRMQTGVENIPPPHKAAEDSNGLDTCKH 180
           +V LV  +  P L PIN   K  +  E S+ +DR+ +  E + PP    E         H
Sbjct: 121 RVTLVPPSDSPELSPINTP-KQGAVFEDSILKDRLYSQSETLAPPQYEGEIVKEPRMVGH 180

Query: 181 IDELRPVD-TPELLSPPYKVAEGVEKSDTCKD-------SDENRAAEDVS---TRQNEDV 240
            DEL+  D   EL +P     + VE   T  D        D +R A++      R ++D 
Sbjct: 181 -DELKAADNAKELKTPKMATVDFVEDRYTANDLKATKDSYDSSRMAKETGFDPIRSHKDA 240

Query: 241 -----------VAEPIENIETRPAE-----GI---KESKLAKD----------------L 300
                      +  P++     P +     GI    E K++K+                L
Sbjct: 241 DDGRAIKATTNLDAPMKKAMDLPRDQGFTNGIAVDSEPKISKERDVVQLQKTDGQNVRGL 300

Query: 301 PELNLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKRDLETLR-KTGQ 340
            EL L K+ +E+K K+  ++++L++A++TI +L +ER I++Q R+ L+ +L  LR K   
Sbjct: 301 DELKLVKDIEEMKLKVDALESKLKQADSTISKLMEERSISSQHRQSLQHELAELRTKKIV 360

BLAST of HG10012609 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.9e-39
Identity = 71/128 (55.47%), Postives = 99/128 (77.34%), Query Frame = 0

Query: 4   ELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKPKDT 63
           EL  ++P +L+F FELKKQ SC + L NK++ +VAFKVKTT+PKKYCVRPNTG++ P+ T
Sbjct: 21  ELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRST 80

Query: 64  CDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKLKVF 123
           C+  VTM AQ+ AP DMQCKDKFL+QG + SPG + +++T ++F+K++G  +EE KL+V 
Sbjct: 81  CEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVT 140

Query: 124 LVSATPPP 132
            V+   PP
Sbjct: 141 YVAPPRPP 148

BLAST of HG10012609 vs. TAIR 10
Match: AT3G60600.3 (vesicle associated protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.9e-39
Identity = 71/128 (55.47%), Postives = 99/128 (77.34%), Query Frame = 0

Query: 4   ELFEIQPAELKFTFELKKQSSCLIQLINKSEQHVAFKVKTTSPKKYCVRPNTGIIKPKDT 63
           EL  ++P +L+F FELKKQ SC + L NK++ +VAFKVKTT+PKKYCVRPNTG++ P+ T
Sbjct: 21  ELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRST 80

Query: 64  CDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKLKVF 123
           C+  VTM AQ+ AP DMQCKDKFL+QG + SPG + +++T ++F+K++G  +EE KL+V 
Sbjct: 81  CEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVT 140

Query: 124 LVSATPPP 132
            V+   PP
Sbjct: 141 YVAPPRPP 148

BLAST of HG10012609 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 77/130 (59.23%), Postives = 101/130 (77.69%), Query Frame = 0

Query: 2   TMELFEIQPAELKFTFELKKQSSCLIQLINK-SEQHVAFKVKTTSPKKYCVRPNTGIIKP 61
           T +L  I P ELKF FELKKQSSC +QL NK + Q VAFKVKTT+P+KYCVRPNTG++ P
Sbjct: 3   TGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLP 62

Query: 62  KDTCDFTVTMLAQRTAPPDMQCKDKFLVQGTVISPGTSEEDITSDVFAKDSGKHIEEKKL 121
            D+C+ TVTM AQ+ AP DMQCKDKFLVQ  V+S GT+ +++ +++F K++G+ IE+ KL
Sbjct: 63  GDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKL 122

Query: 122 KVFLVSATPP 131
           +V  + A PP
Sbjct: 123 RVVYIPANPP 132


HSP 2 Score: 32.7 bits (73), Expect = 6.9e-01
Identity = 21/83 (25.30%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 258 NLTKNFQELKSKLTLMDAELQEAEATIMRLKKERMITTQEREMLKRDLETLRKTGQRSIQ 317
           ++++ F+E   K +       EA + I +L +E+   TQ+ + L+ +LE LRK   +   
Sbjct: 162 DVSRTFEETSEKSS-------EAWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQS 221

Query: 318 VGFPLMYVVMVAFISLLVGYFID 341
            G  L+ +++V  +  ++GY ++
Sbjct: 222 GGHSLLLMLLVGLLGCVIGYLLN 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902396.11.5e-17092.96vesicle-associated protein 2-2-like isoform X1 [Benincasa hispida] >XP_038902435... [more]
XP_008438789.14.2e-16591.20PREDICTED: vesicle-associated protein 2-2-like isoform X1 [Cucumis melo][more]
XP_004134182.17.8e-16490.32vesicle-associated protein 2-2 [Cucumis sativus] >XP_011651023.1 vesicle-associa... [more]
XP_023539031.13.8e-15886.22vesicle-associated protein 1-2-like [Cucurbita pepo subsp. pepo][more]
XP_022939166.13.8e-15885.92vesicle-associated protein 1-2-like [Cucurbita moschata] >XP_022939172.1 vesicle... [more]
Match NameE-valueIdentityDescription
B9DHD71.1e-5638.60Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Q84WW53.4e-3759.23Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q8VZ956.5e-3632.14Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SHC88.5e-3654.20Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q9LVU11.4e-3559.35Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3AXX02.0e-16591.20vesicle-associated protein 2-2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0L7T63.8e-16490.32MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G151410 PE=4 SV... [more]
A0A6J1FF591.8e-15885.92vesicle-associated protein 1-2-like OS=Cucurbita moschata OX=3662 GN=LOC11144515... [more]
A0A6J1I9H35.3e-15886.51vesicle-associated protein 1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111472319 ... [more]
A0A5D3CXC69.1e-15891.64Vesicle-associated protein 2-2-like isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT1G08820.18.1e-5838.60vamp/synaptobrevin-associated protein 27-2 [more]
AT1G08820.28.1e-5838.60vamp/synaptobrevin-associated protein 27-2 [more]
AT3G60600.24.9e-3955.47vesicle associated protein [more]
AT3G60600.34.9e-3955.47vesicle associated protein [more]
AT4G00170.12.4e-3859.23Plant VAMP (vesicle-associated membrane protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 263..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 201..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..227
NoneNo IPR availablePANTHERPTHR10809:SF105VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 1..339
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 2..127
e-value: 6.4E-42
score: 144.5
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..340
e-value: 1.4E-52
score: 177.3
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..339
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 6..109
e-value: 4.8E-31
score: 106.8
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 5..125
score: 28.831718
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 5..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012609.1HG10012609.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane