HG10012531 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012531
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr01: 22161531 .. 22164108 (-)
RNA-Seq ExpressionHG10012531
SyntenyHG10012531
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGTATTTTTTTTTTCTTCTTCTTGATTTGCAAGTGGTTGCATCAATTCCTACATCTTTTCATTTGCTCTTTAACTTGAATCTGCAATTCTGGGGATTTGTACACTTTTTGTTTTTCAATGTACTTATGGTTCTAGTTGTATTTGGCTTGCTTTCTTTTCTACCGTTGTTGTCTTTTTTTTTTATCTGTTGTAGTTGCTTGATTTAATCAATTCATGGTGCTGCCCCTAAGTTTCTTTTGGTTTTTTTTCTTTAGGTGGCATTGCTTTTGTTGCTACAATTGAGCAATGCTGCGAAAATAATCGGTGAAGAGCTCGCCGGACAGTGCGATTCTAAACCAACGTTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGCGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCATCTTACCCTCTTCTTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGTATGTTCTTCTTCATGGATGGTGCTCAAACAAGTAGTTGTTCCTAGGCAGTTGTTTTTAAACATATTGATCCTCTCTTTAACAGGACCCATTACATTGGGAACTTGTCAATCCCTTACCTTCATATGGTAGAGGTATTGAAGTTCCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTTTGTTGCTGCTCTTCTTACCCTTTTGATTCAGAATCTAGTTTGCGGAAATGAATTCCTCTGAATTAGTGTTATTATGTTGATTTTGGATGTGGCTAGGTGATGATGGGGTGATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTACAGCTCTCATTCCTTAAACTACAGCCGCCCTCATCTCGTGGAATTTGAGGATTCCCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGGTATGCTATAACAACCAGGGATTAGTTTCTTCTGCCTCTACTGATTCTCTGTTCACCTTGTTGATATGTCTATTTGCTGATTGTCAGATAATCTGTATTGAATATAAGTTTTTCATGTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGGTTTGTAATGCTTCTTCAATTTTCAGCATCCTCATTGTTATTTTATGTGACTATTGTTGATGGGCTCTCTCTCTCTCTCATTCGCTCTCTCATATGATGTGATACATCCTTTGGGTGAATGGCGCAAGCACCATTGAAGAATCTCGCCCTTTTATCTCACTCCCACACGCGCACACACACCGCATAGTGCTCAGAAGAAGATGCCATCAAGAAAGCTATCATGAGTAGTTGACTGTCAACAGATATGTTTATAAATTTTAGTTGATATCAACACTTGTTTAACATCATGTAATCTAAGAAAAGCAATGTCTTCTAATAAAGTCCCAATGTACCAAGTTCTTATATTTGGGGCCTGAGGTCTGTGGTAATTTTTACAATAAAATTTAAGAAACTTAGCGTTGTTAAAATGGTGAAGTTGGATTGAATTTAGAAATAAGATTCTGTTAGGAGTTTATGACACGTAGTGGGATGCCATCATGATCAAAAGGATTTATTGTGGGGTGGACCAAATTTAGACATACAGTACATCATGGATTTCATTAATGACCATTAATCCAAGAATTAAATTCAGTAGTATCTGATTCATTCTTTCTCGTTTCTAATTAATGAGGTGGAGGCATTCTTTTATAAATAAAGTTTGATTGGTGCTTTCAGATTCGTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTGCAACTGAACAACTCATTTATTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCAGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTCGGGTCACATCCGGACGATCAGTTTGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACAAACATTAAAATTGCTGGAAACTTTTCTGGAATACAAGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAAACTGCTGTTTTATGA

mRNA sequence

ATGTTGGTGGCATTGCTTTTGTTGCTACAATTGAGCAATGCTGCGAAAATAATCGGTGAAGAGCTCGCCGGACAGTGCGATTCTAAACCAACGTTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGCGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCATCTTACCCTCTTCTTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGACCCATTACATTGGGAACTTGTCAATCCCTTACCTTCATATGGTAGAGGTATTGAAGTTCCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTGATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTACAGCTCTCATTCCTTAAACTACAGCCGCCCTCATCTCGTGGAATTTGAGGATTCCCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCGTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTGCAACTGAACAACTCATTTATTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCAGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTCGGGTCACATCCGGACGATCAGTTTGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACAAACATTAAAATTGCTGGAAACTTTTCTGGAATACAAGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAAACTGCTGTTTTATGA

Coding sequence (CDS)

ATGTTGGTGGCATTGCTTTTGTTGCTACAATTGAGCAATGCTGCGAAAATAATCGGTGAAGAGCTCGCCGGACAGTGCGATTCTAAACCAACGTTAGATCCAAGACCACACAGTGTGTCTATCTTGGAGTTTGGCGCTGTTGGAGATGGCAAAACTCTTAACACCATTGCCTTCCAAAATGCCATTTTCTATCTTAAATCCTTTGCTGATAAGGGTGGAGCTCAGCTTTATGTTCCACCTGGAAAATGGCTCACTGGAAGCATTAATCTTACCAGCCATCTTACCCTCTTCTTGGAAAAAGGTGCTGTGATTCTTGGCTCTCAGGACCCATTACATTGGGAACTTGTCAATCCCTTACCTTCATATGGTAGAGGTATTGAAGTTCCAGGAAAACGATACCGGAGCTTGATAAATGGTTACAATTTACAAGATGTTGTAATAACAGGTGATGATGGGGTGATTGATGGACAGGGTTTAGTTTGGTGGAATTGGTACAGCTCTCATTCCTTAAACTACAGCCGCCCTCATCTCGTGGAATTTGAGGATTCCCAATATGTAGTTGTTTCAAACCTCACTTTCTTGAATACTCCTGCATATAACATTCATCCAGTCTATTGCAGTAACGTCTATGTTTACAACATCTCTGTCTCTGCACCTTCTGAATCTCCCTACACAGTTGGAATAGTCCCAGATTCGTCCGATCATGTATGCATAGAGGGCTGCAACATTGCCACAGGATATGATGCCATTGCCTTGAAGAGTGGTTGGGATCAGTATGGTATTGCCTATGGCAGACCATCTAAAAACATACACATTAGAAGGGTCCACCTTCAGTCATCTTCAGGTTCTTCCATTGCCTTTGGTAGTGAGATGTCTGGTGGGATATCTAATGTTCTTGTGGAGCATGTGCAACTGAACAACTCATTTATTGGCATTCAATTTAGAACTACCAAGGGAAGAGGGGGTTACATTAAAGGAATAGTTGTTTCAGATGTTGAAATGGAAAACATATCCACGGCATTCAGTGCCTCTGGTCACTTCGGGTCACATCCGGACGATCAGTTTGATCCTAATGCTCTTCCAATTGTGCAGGACATAACCTTGCAGAATGTGAGAGGCACAAACATTAAAATTGCTGGAAACTTTTCTGGAATACAAGAATCTCCCTTCACTTCAATCTATTTATCCAACATTACCTTTTCAATCAATTCATCTTCTTCCACTTCTTGGATCTGTTCAGATGTTTCTGGCTTTTCAGAATCTGTGATCCCACCACCCTGTTCTGATCTCAGTACTCCATATTCAATTTCTTCCTCAGCAGCCTCTCCCCTTGTGAATTCAACTGGAAAAACTGCTGTTTTATGA

Protein sequence

MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Homology
BLAST of HG10012531 vs. NCBI nr
Match: XP_038881588.1 (probable polygalacturonase isoform X5 [Benincasa hispida])

HSP 1 Score: 899.8 bits (2324), Expect = 9.5e-258
Identity = 447/454 (98.46%), Postives = 450/454 (99.12%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 120

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 180

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG
Sbjct: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGIQFRT KGRGGYIKGIVVSDVEMENISTAFSASGHF SHPDD+FDPNAL
Sbjct: 301 EHVQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPNAL 360

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSW+CSDVSGFSE
Sbjct: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGFSE 420

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNS GKTAVL
Sbjct: 421 SVIPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 454

BLAST of HG10012531 vs. NCBI nr
Match: XP_038881575.1 (probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probable polygalacturonase isoform X4 [Benincasa hispida])

HSP 1 Score: 899.8 bits (2324), Expect = 9.5e-258
Identity = 447/454 (98.46%), Postives = 450/454 (99.12%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 3   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 62

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 63  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 122

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 123 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 182

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG
Sbjct: 183 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 242

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 243 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 302

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGIQFRT KGRGGYIKGIVVSDVEMENISTAFSASGHF SHPDD+FDPNAL
Sbjct: 303 EHVQLNNSFIGIQFRTAKGRGGYIKGIVVSDVEMENISTAFSASGHFESHPDDEFDPNAL 362

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSW+CSDVSGFSE
Sbjct: 363 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWLCSDVSGFSE 422

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNS GKTAVL
Sbjct: 423 SVIPPPCSDLSTPYSISSSAASPLVNSNGKTAVL 456

BLAST of HG10012531 vs. NCBI nr
Match: XP_004134229.1 (probable polygalacturonase isoform X2 [Cucumis sativus] >XP_011651066.1 probable polygalacturonase isoform X2 [Cucumis sativus])

HSP 1 Score: 899.8 bits (2324), Expect = 9.5e-258
Identity = 446/454 (98.24%), Postives = 450/454 (99.12%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           +LVALLL LQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 4   LLVALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 63

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 64  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 123

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 124 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 183

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG
Sbjct: 184 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 243

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 244 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 303

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGIQ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNAL
Sbjct: 304 EHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 363

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE
Sbjct: 364 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 423

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 424 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of HG10012531 vs. NCBI nr
Match: XP_008438914.1 (PREDICTED: probable polygalacturonase isoform X2 [Cucumis melo])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 445/454 (98.02%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           +LVALLL LQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 4   LLVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 63

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 64  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 123

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 124 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 183

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEG
Sbjct: 184 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEG 243

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 244 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 303

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGI+FRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNAL
Sbjct: 304 EHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 363

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE
Sbjct: 364 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 423

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 424 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of HG10012531 vs. NCBI nr
Match: XP_011651065.1 (probable polygalacturonase isoform X1 [Cucumis sativus] >KAE8650399.1 hypothetical protein Csa_009981 [Cucumis sativus])

HSP 1 Score: 898.7 bits (2321), Expect = 2.1e-257
Identity = 445/452 (98.45%), Postives = 448/452 (99.12%), Query Frame = 0

Query: 3   VALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 62
           VALLL LQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 63  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLPSY 122
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 123 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEFED 182
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 183 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 242
           SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 243 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 302
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 303 VQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNALPI 362
           VQLNNSFIGIQ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNALPI
Sbjct: 307 VQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 363 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 422
           VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 423 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of HG10012531 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.3e-112
Identity = 205/431 (47.56%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           +LVA++LLL +S      G  L  +      +  R HS S+++FG VGDG+TLNT AFQ+
Sbjct: 27  VLVAVVLLLSVSRGECRKGRIL--EALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQD 86

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           A+  L  +  +GGAQLYVP GKWLTGS +LTSH TLFL + AV+L SQD   W ++ PLP
Sbjct: 87  AVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPLP 146

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRG +    RY SLI G NL DV+ITGD+G IDGQG +WW  +    L Y+RP+L+E 
Sbjct: 147 SYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIEL 206

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
             S  + +SNLT LN+P++N+HPVY  N+ +  I++ AP  SP T GI PDS  +  IE 
Sbjct: 207 MYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTRIED 266

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           C I +G D +A+KSGWD+YGIAYG P+K + IRR+   S   + IA GSEMSGGI +V  
Sbjct: 267 CYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRA 326

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           E +   NS  GI+ +T  GRGGY+K I V  + M+ +  AF  +G++GSH D+ +DP A 
Sbjct: 327 EDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAF 386

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINS-SSSTSWICSDVSGFS 420
           P++Q I  +++   N+ +A    GI   PFT I +SN+T  + + +    W C+DV G S
Sbjct: 387 PVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVEGIS 446

Query: 421 ESVIPPPCSDL 431
             V P PCS L
Sbjct: 447 SGVTPTPCSTL 455

BLAST of HG10012531 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-25
Identity = 112/407 (27.52%), Postives = 182/407 (44.72%), Query Frame = 0

Query: 37  HSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTL 96
           +++ + +FGAVGDG T ++ AF  A  +    +  G  Q  VP G        +T  L  
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG--------MTFMLQP 81

Query: 97  FLEKGAVILGSQDPLHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDG 156
              +G+       P+  +++  L +  +G     K    L    +++ +VI G DG I+G
Sbjct: 82  LKFQGSC---KSTPVFVQMLGKLVAPSKGNWKGDKDQWILFT--DIEGLVIEG-DGEING 141

Query: 157 QGLVWWNWYSSHSLNYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV 216
           QG  WW          SRP  ++F     + +S LT L++P  +IH   C+ V + ++ +
Sbjct: 142 QGSSWWEHKG------SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRI 201

Query: 217 SAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVH 276
           +AP  SP T GI   +S +V I+ C IATG D IA+ SG           + NIHI  + 
Sbjct: 202 NAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNIHISGI- 261

Query: 277 LQSSSGSSIAFGSEMSGG----ISNVLVEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDV 336
                G  I+ GS    G    + NV V++     +  G + +T +G  GY + I  + +
Sbjct: 262 -DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGI 321

Query: 337 EMENISTAFSASGHF-GSHPDDQFDPNALPI-VQDITLQNVRGTNIKIAG-NFSGIQESP 396
            ++N+         + G   D+  D  +  + V  +   N  GT+    G +F   +  P
Sbjct: 322 TLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVP 381

Query: 397 FTSIYLSNITFSINSSSS---TSWICSDVSGFSESVIPP-PCSDLST 433
            T I+L ++     SS S       C +V G S   +P   C +LST
Sbjct: 382 CTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELST 393

BLAST of HG10012531 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 2.0e-24
Identity = 105/402 (26.12%), Postives = 184/402 (45.77%), Query Frame = 0

Query: 36  PHSVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGK-WLTGSINLTSHL 95
           P +VS+ +FGA GDGKT +T AF NA  + K+ +  G   L VP G  +L  SI LT   
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 96  TLFLEKGAVILGSQDPLHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVI 155
              L               ++   L +  +  +        + +G N    V  GD GV+
Sbjct: 125 NSILTV-------------QIFGTLSASQKRSDYKDISKWIMFDGVNNLS-VDGGDTGVV 184

Query: 156 DGQGLVWWNWYSSHSLNYSR-----PHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNV 215
           DG G  WW   +S   N ++     P  + F +S+ ++V NL   N     I    CSNV
Sbjct: 185 DGNGETWWQ--NSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNV 244

Query: 216 YVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKN 275
            V N+ V+AP++SP T GI   ++ ++ +    I TG D I+++SG           S+N
Sbjct: 245 QVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQN 304

Query: 276 IHIRRVHLQSSSGSSI-AFGSEMSGG-ISNVLVEHVQLNNSFIGIQFRTTKGRGGYIKGI 335
           + I  +      G SI + G + S   +S V V+  +L+ +  G++ +T +G  G    I
Sbjct: 305 VQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNI 364

Query: 336 VVSDVEMENISTAFSASGHFGSHPDDQFDPNALPIVQDITLQNVRGTNI-KIAGNFSGIQ 395
           +  +++M+N+         +        + +A+  V+++  +++ GT+  + A  F+  +
Sbjct: 365 IFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNCSK 424

Query: 396 ESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCS 429
             P   I L  +  +I    +T   C++ +   +  + P C+
Sbjct: 425 NYPCQGIVLDRV--NIKGGKAT---CTNANVVDKGAVLPQCN 431

BLAST of HG10012531 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 4.9e-23
Identity = 105/406 (25.86%), Postives = 178/406 (43.84%), Query Frame = 0

Query: 40  SILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPG-KWLTGSINLTS----HL 99
           ++ ++GAVGDGK   T AF  A    ++   K  A L VP   K++  ++        H 
Sbjct: 60  NVEKYGAVGDGKHDCTEAFSTA---WQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHF 119

Query: 100 TLFLEKGAVILGSQDPLHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVI 159
           T  ++   +I   Q+P  W                 K  R  +    L    + G  GVI
Sbjct: 120 TFKVD--GIIAAYQNPASW-----------------KNNRIWLQFAKLTGFTLMG-KGVI 179

Query: 160 DGQGLVWW----NWYSSHSL--NYSRPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSN 219
           DGQG  WW     W +   +  +  RP  ++F+ S  +++  L  +N+P +++    C  
Sbjct: 180 DGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEG 239

Query: 220 VYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSK 279
           V +  IS++AP +SP T GI   +S +  ++   I TG D +A+ +G           S 
Sbjct: 240 VKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTG-----------SS 299

Query: 280 NIHIRRVHLQSSSGSSI-AFGSEMS-GGISNVLVEHVQLNNSFIGIQFRTTKGRGGYIKG 339
           NI I  +      G SI + G E S   +S V V   +  ++  G++ +T +G  G    
Sbjct: 300 NIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASH 359

Query: 340 IVVSDVEMENISTAFSASGHFGSHPDDQFDPNALPIVQDITLQNVRGTNIKIAGNFSGIQ 399
           I+  +VEM N       +  + +      +  +   +QD+T +N+RGT+   A       
Sbjct: 360 IIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCS 419

Query: 400 ES-PFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSDLS 432
           +S P   I LS+I+  + S    S +  + +G+    + P C +LS
Sbjct: 420 DSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLS 431

BLAST of HG10012531 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 3.0e-20
Identity = 94/403 (23.33%), Postives = 173/403 (42.93%), Query Frame = 0

Query: 38  SVSILEFGAVGDGKTLNTIAFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLT-- 97
           +V++ +FGA GDG+  +T AF+ A  +  + +    A L VP   +L   I+ +      
Sbjct: 89  TVNVDDFGAKGDGRD-DTKAFEKA--WKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSG 148

Query: 98  LFLEKGAVILGSQDPLHWELVNPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVID 157
           L ++    I  S D   +                 K  R  +   ++Q++ + G  G I+
Sbjct: 149 LTMQIYGTIEASDDRSDYR----------------KDGRHWLVFDSVQNLRVEG-GGTIN 208

Query: 158 GQGLVWWNWYSSHSLNYS-----RPHLVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVY 217
           G G +WW   +S   N +      P  + F  S++V+V NL   N    ++    C NV 
Sbjct: 209 GNGKIWWQ--NSCKTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQ 268

Query: 218 VYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNI 277
             N+ V+AP  SP T GI    + ++ I  C I TG D I++ +G           S+ +
Sbjct: 269 ASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNG-----------SRKV 328

Query: 278 HIRRVHLQSSSG---SSIAFGSEMSGGISNVLVEHVQLNNSFIGIQFRTTKGRGGYIKGI 337
            +  +      G    S+ +G+     +S+V+V   +L  +  G++ +T +G  G    I
Sbjct: 329 RVNDITCGPGHGISIGSLGYGNS-EAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNI 388

Query: 338 VVSDVEMENISTAFSASGHFGSHPDDQFDPNALPIVQDITLQNVRGT-NIKIAGNFSGIQ 397
              +VEM N+        ++        + ++   V+++  QN++GT    +A  F   +
Sbjct: 389 KFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSK 448

Query: 398 ESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPPCSD 430
             P   I L ++   I   ++   +C++V      V+ P C +
Sbjct: 449 RFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSPHCQE 457

BLAST of HG10012531 vs. ExPASy TrEMBL
Match: A0A0A0LAN4 (Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165090 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 4.6e-258
Identity = 446/454 (98.24%), Postives = 450/454 (99.12%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           +LVALLL LQLSNAAKIIGEEL GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 4   LLVALLLFLQLSNAAKIIGEELVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 63

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 64  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 123

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 124 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 183

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG
Sbjct: 184 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 243

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 244 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 303

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGIQ RTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNAL
Sbjct: 304 EHVQLNNSFIGIQIRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 363

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE
Sbjct: 364 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 423

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 424 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of HG10012531 vs. ExPASy TrEMBL
Match: A0A1S3AXL3 (probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.9e-258
Identity = 445/454 (98.02%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           +LVALLL LQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 4   LLVALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 63

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLP
Sbjct: 64  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLP 123

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEF
Sbjct: 124 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEF 183

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
           EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEG
Sbjct: 184 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEG 243

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
           CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV
Sbjct: 244 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 303

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           EHVQLNNSFIGI+FRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNAL
Sbjct: 304 EHVQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNAL 363

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE
Sbjct: 364 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 423

Query: 421 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 424 SVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 457

BLAST of HG10012531 vs. ExPASy TrEMBL
Match: A0A1S3AX49 (probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.8e-257
Identity = 444/452 (98.23%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 3   VALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 62
           VALLL LQLSNAA+IIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI
Sbjct: 7   VALLLFLQLSNAARIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQNAI 66

Query: 63  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLPSY 122
           FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELVNPLPSY
Sbjct: 67  FYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVNPLPSY 126

Query: 123 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEFED 182
           GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHLVEFED
Sbjct: 127 GRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHLVEFED 186

Query: 183 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCN 242
           SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISV APSESPYTVGIVPDSSDHVCIEGCN
Sbjct: 187 SQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVYAPSESPYTVGIVPDSSDHVCIEGCN 246

Query: 243 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 302
           IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH
Sbjct: 247 IATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEH 306

Query: 303 VQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNALPI 362
           VQLNNSFIGI+FRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDD++DPNALPI
Sbjct: 307 VQLNNSFIGIEFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDEYDPNALPI 366

Query: 363 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 422
           VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV
Sbjct: 367 VQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESV 426

Query: 423 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL
Sbjct: 427 IPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 458

BLAST of HG10012531 vs. ExPASy TrEMBL
Match: A0A6J1GUW5 (probable polygalacturonase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457693 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.0e-244
Identity = 428/457 (93.65%), Postives = 442/457 (96.72%), Query Frame = 0

Query: 1   MLVA-LLLLLQLSNAAKIIGEEL--AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 60
           MLVA LLLLLQLSNA KI+GEEL   GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA
Sbjct: 4   MLVALLLLLLQLSNATKIVGEELEVVGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIA 63

Query: 61  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVN 120
           FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQ+P HWELVN
Sbjct: 64  FQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQNPSHWELVN 123

Query: 121 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHL 180
           PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNW+SSHSLNYSRPHL
Sbjct: 124 PLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWFSSHSLNYSRPHL 183

Query: 181 VEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVC 240
           VEFEDS+Y+VVSNLTFLNTPAYNIHPVYCSNVY+YNISVSAPSESP+TVGIVPDSSDHVC
Sbjct: 184 VEFEDSKYIVVSNLTFLNTPAYNIHPVYCSNVYIYNISVSAPSESPHTVGIVPDSSDHVC 243

Query: 241 IEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 300
           IEG NIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN
Sbjct: 244 IEGSNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISN 303

Query: 301 VLVEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDP 360
           VLVEHVQL+NS   IQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFG HPDD+FDP
Sbjct: 304 VLVEHVQLHNSSTAIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGLHPDDEFDP 363

Query: 361 NALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSG 420
           NALPIVQDITLQNVRG+NIKIAGNFSGIQESPFTSIYLSNITF I+SSSS+SWICSDVSG
Sbjct: 364 NALPIVQDITLQNVRGSNIKIAGNFSGIQESPFTSIYLSNITFLISSSSSSSWICSDVSG 423

Query: 421 FSESVIPPPCSDLSTPYSISSSAASPLVNSTGKTAVL 455
           FS+SVIPPPCSDLS PYSISSSAASPL+NS  KTAVL
Sbjct: 424 FSDSVIPPPCSDLSHPYSISSSAASPLLNSARKTAVL 460

BLAST of HG10012531 vs. ExPASy TrEMBL
Match: A0A6J1C871 (probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111009229 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.3e-244
Identity = 426/456 (93.42%), Postives = 443/456 (97.15%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEEL-AGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQ 60
           MLVALLLLLQLSNAA+IIGEEL  GQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQ
Sbjct: 3   MLVALLLLLQLSNAARIIGEELNGGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQ 62

Query: 61  NAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPL 120
           NAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDP HWELV+PL
Sbjct: 63  NAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPSHWELVDPL 122

Query: 121 PSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVE 180
           PSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGV+DGQG VWW+WYSSHSLNYSRPHLVE
Sbjct: 123 PSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVVDGQGSVWWDWYSSHSLNYSRPHLVE 182

Query: 181 FEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIE 240
           FEDS+YVVVSNLTFLNTPAYNIHPVYCS+VYVYNISVSAP ESPYTVGIVPDSSDHVCIE
Sbjct: 183 FEDSKYVVVSNLTFLNTPAYNIHPVYCSDVYVYNISVSAPPESPYTVGIVPDSSDHVCIE 242

Query: 241 GCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVL 300
           GCNIATGYDAIALKSGWDQYGIAYGRPS+NIHIRRVHLQSSSGSSIAFGSEMSGGIS+VL
Sbjct: 243 GCNIATGYDAIALKSGWDQYGIAYGRPSRNIHIRRVHLQSSSGSSIAFGSEMSGGISSVL 302

Query: 301 VEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNA 360
           VEHV L NS  G+QFRT KGRGG+IKGIV+SDVEMENI+TAFSASGHFG HPDD+FDPNA
Sbjct: 303 VEHVHLYNSSSGVQFRTAKGRGGFIKGIVISDVEMENIATAFSASGHFGLHPDDEFDPNA 362

Query: 361 LPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFS 420
           LPIVQDITLQNVRGTNI+IAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFS
Sbjct: 363 LPIVQDITLQNVRGTNIQIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFS 422

Query: 421 ESVIPPPCSDLSTPYSISSS-AASPLVNSTGKTAVL 455
           +SVIPPPCSDLSTP+SISSS AAS LVNS+GKTAVL
Sbjct: 423 DSVIPPPCSDLSTPFSISSSAAASSLVNSSGKTAVL 458

BLAST of HG10012531 vs. TAIR 10
Match: AT3G06770.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 569.3 bits (1466), Expect = 2.7e-162
Identity = 277/434 (63.82%), Postives = 346/434 (79.72%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGE-ELAGQ-CD--SKPTLDPRPHSVSILEFGAVGDGKTLNTI 60
           M V  +L+L L++   I      AG+ CD   + +L  RPHSVSI +FGAVGDGKTLNT+
Sbjct: 1   MSVVTILILALTSVIPIYANVHGAGKICDELGRRSLSTRPHSVSITDFGAVGDGKTLNTL 60

Query: 61  AFQNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELV 120
           AFQNA+FYL SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GAVI+ SQDP HWE+V
Sbjct: 61  AFQNAVFYLMSFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVV 120

Query: 121 NPLPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPH 180
           +PLPSYGRGI++PGKRY+SLING  L DVV+TGD+G IDGQGLVWW+ ++SHSL Y+RPH
Sbjct: 121 DPLPSYGRGIDLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPH 180

Query: 181 LVEFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHV 240
           L+EF  S+ V+VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+V
Sbjct: 181 LIEFLSSENVIVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYV 240

Query: 241 CIEGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGIS 300
           CI+   I  GYDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS
Sbjct: 241 CIQNSTINVGYDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGIS 300

Query: 301 NVLVEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFD 360
           +V+V++  ++ S  GI FRTTKGRGGYIK I +S+++M  I TA  A+G FGSHPDD++D
Sbjct: 301 DVVVDNAHIHYSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYD 360

Query: 361 PNALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVS 420
            NALP+V  I L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V 
Sbjct: 361 VNALPLVSHIRLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVY 420

Query: 421 GFSESVIPPPCSDL 431
           G SESVIP PC +L
Sbjct: 421 GSSESVIPEPCPEL 434

BLAST of HG10012531 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 567.4 bits (1461), Expect = 1.0e-161
Identity = 273/436 (62.61%), Postives = 340/436 (77.98%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEELAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 60
           M VAL+ LL  +    I GE     C     LDPRPHSVSILEFGAVGDGKTLNTIAFQN
Sbjct: 3   MPVALVWLLAFT-ILLISGEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIAFQN 62

Query: 61  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 120
           A+FYLKSFADKGGAQLYVPPGKWLTGS NLTSHLTLFLEKGA IL S DP HW++V+PLP
Sbjct: 63  AVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVSPLP 122

Query: 121 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 180
           SYGRGIE+PGKRYRSLING NL DVVITG++G  DGQG  WW W  S SLNYSRPH++EF
Sbjct: 123 SYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHIIEF 182

Query: 181 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 240
             S+++++SNLTFLN PA NIHPVYCS +++  + +    +SP+ +G+ PDSSD+VCIE 
Sbjct: 183 VSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVCIED 242

Query: 241 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 300
             I  G+DA++LKSGWDQYGI YGRP+  +HIR + L+S +G+ I+FGSEMSGG+S+V V
Sbjct: 243 STINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDVTV 302

Query: 301 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 360
           E + +++S IG+ FRTT+GRGGYI+ I +SDV++ ++ TA  A+GH GSHPDD+FD +AL
Sbjct: 303 ERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRDAL 362

Query: 361 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSE 420
           P+V  I ++N  G +I +AGN +GI ESPFTSI L++I   + + S  SWICS+VSGFS+
Sbjct: 363 PVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADI--HLQTRSEESWICSNVSGFSD 422

Query: 421 SVIPPPCSDL-STPYS 436
            V P PC +L S+P S
Sbjct: 423 DVSPEPCQELMSSPSS 435

BLAST of HG10012531 vs. TAIR 10
Match: AT5G49215.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 273/434 (62.90%), Postives = 334/434 (76.96%), Query Frame = 0

Query: 3   VALLLLLQLSNAAKIIGEE--LAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAFQN 62
           VAL+LLL LS+   I G    +   CD       RPHSV I +FGAVGDGKTLNT+AFQN
Sbjct: 5   VALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAFQN 64

Query: 63  AIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLP 122
           A+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK AVIL SQDP HW++ + LP
Sbjct: 65  AVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDALP 124

Query: 123 SYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEF 182
           SYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWW+ ++SHSL YSRPHLVEF
Sbjct: 125 SYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLVEF 184

Query: 183 EDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEG 242
             ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++VCI+ 
Sbjct: 185 VSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCIQE 244

Query: 243 CNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLV 302
            +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS+V V
Sbjct: 245 SSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDVEV 304

Query: 303 EHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNAL 362
               ++NS  GI FRTT GRGGYIK I +S++ M N+ TAF A+G FG+HPD  FD NA 
Sbjct: 305 SDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDENAY 364

Query: 363 PIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSS--TSWICSDVSGF 422
           P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS V G 
Sbjct: 365 PLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVDGS 424

Query: 423 SESVIPPPCSDLST 433
           SE V+P PC +L +
Sbjct: 425 SEFVVPEPCLELKS 438

BLAST of HG10012531 vs. TAIR 10
Match: AT5G49215.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.6e-157
Identity = 274/436 (62.84%), Postives = 335/436 (76.83%), Query Frame = 0

Query: 1   MLVALLLLLQLSNAAKIIGEE--LAGQCDSKPTLDPRPHSVSILEFGAVGDGKTLNTIAF 60
           M VAL+LLL LS+   I G    +   CD       RPHSV I +FGAVGDGKTLNT+AF
Sbjct: 1   MPVALVLLLTLSSVILINGRSYGVGNICDRGRRPSERPHSVKITDFGAVGDGKTLNTLAF 60

Query: 61  QNAIFYLKSFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNP 120
           QNA+FYLKSFADKGGAQLYVPPG+WLTGS NLTSHLTLFLEK AVIL SQDP HW++ + 
Sbjct: 61  QNAVFYLKSFADKGGAQLYVPPGRWLTGSFNLTSHLTLFLEKDAVILASQDPSHWQVTDA 120

Query: 121 LPSYGRGIEVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLV 180
           LPSYGRGI++PGKRY SLING  L DVV+TGD+G IDGQGLVWW+ ++SHSL YSRPHLV
Sbjct: 121 LPSYGRGIDLPGKRYMSLINGDMLHDVVVTGDNGTIDGQGLVWWDRFNSHSLEYSRPHLV 180

Query: 181 EFEDSQYVVVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCI 240
           EF  ++ V+VSNLTFLN PAY IH VYC N+Y++ ++ +   ESPYT+GIVPDSS++VCI
Sbjct: 181 EFVSAENVIVSNLTFLNAPAYTIHSVYCRNLYIHRVTANTCPESPYTIGIVPDSSENVCI 240

Query: 241 EGCNIATGYDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNV 300
           +  +I  GYDAI+LKSG D+YG++Y RP+ N+ IR V+L+++SGSSI+FGSEMSGGIS+V
Sbjct: 241 QESSINMGYDAISLKSGCDEYGLSYARPTANVQIRNVYLRAASGSSISFGSEMSGGISDV 300

Query: 301 LVEHVQLNNSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPN 360
            V    ++NS  GI FRTT GRGGYIK I +S++ M N+ TAF A+G FG+HPD  FD N
Sbjct: 301 EVSDAHIHNSLSGIAFRTTNGRGGYIKEIDISNIHMVNVGTAFLANGSFGTHPDSGFDEN 360

Query: 361 ALPIVQDITLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSS--TSWICSDVS 420
           A P+V  I L ++ G NI  AG F G +ESPFTSI LSNI+ SI +S+S   SW CS V 
Sbjct: 361 AYPLVSHIRLHDIVGENISTAGYFFGTKESPFTSILLSNISLSIKNSASPADSWQCSYVD 420

Query: 421 GFSESVIPPPCSDLST 433
           G SE V+P PC +L +
Sbjct: 421 GSSEFVVPEPCLELKS 436

BLAST of HG10012531 vs. TAIR 10
Match: AT3G06770.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 508.8 bits (1309), Expect = 4.4e-144
Identity = 239/364 (65.66%), Postives = 298/364 (81.87%), Query Frame = 0

Query: 67  SFADKGGAQLYVPPGKWLTGSINLTSHLTLFLEKGAVILGSQDPLHWELVNPLPSYGRGI 126
           SFADKGGAQLYVPPG WLTGS +LTSHLTLFLE GAVI+ SQDP HWE+V+PLPSYGRGI
Sbjct: 2   SFADKGGAQLYVPPGHWLTGSFSLTSHLTLFLENGAVIVASQDPSHWEVVDPLPSYGRGI 61

Query: 127 EVPGKRYRSLINGYNLQDVVITGDDGVIDGQGLVWWNWYSSHSLNYSRPHLVEFEDSQYV 186
           ++PGKRY+SLING  L DVV+TGD+G IDGQGLVWW+ ++SHSL Y+RPHL+EF  S+ V
Sbjct: 62  DLPGKRYKSLINGNKLHDVVVTGDNGTIDGQGLVWWDRFTSHSLKYNRPHLIEFLSSENV 121

Query: 187 VVSNLTFLNTPAYNIHPVYCSNVYVYNISVSAPSESPYTVGIVPDSSDHVCIEGCNIATG 246
           +VSNLTFLN PAY+I+ +Y S+VY++ I   +  +SPYT+GIVPDSSD+VCI+   I  G
Sbjct: 122 IVSNLTFLNAPAYSIYSIYSSHVYIHKILAHSSPKSPYTIGIVPDSSDYVCIQNSTINVG 181

Query: 247 YDAIALKSGWDQYGIAYGRPSKNIHIRRVHLQSSSGSSIAFGSEMSGGISNVLVEHVQLN 306
           YDAI+LKSGWD+YGIAY RP++N+HIR V+L+ +SGSSI+FGSEMSGGIS+V+V++  ++
Sbjct: 182 YDAISLKSGWDEYGIAYSRPTENVHIRNVYLRGASGSSISFGSEMSGGISDVVVDNAHIH 241

Query: 307 NSFIGIQFRTTKGRGGYIKGIVVSDVEMENISTAFSASGHFGSHPDDQFDPNALPIVQDI 366
            S  GI FRTTKGRGGYIK I +S+++M  I TA  A+G FGSHPDD++D NALP+V  I
Sbjct: 242 YSLTGIAFRTTKGRGGYIKEIDISNIDMLRIGTAIVANGSFGSHPDDKYDVNALPLVSHI 301

Query: 367 TLQNVRGTNIKIAGNFSGIQESPFTSIYLSNITFSINSSSSTSWICSDVSGFSESVIPPP 426
            L N+ G NI IAG   GI+ESPF+S+ LSN++ S++S SS SW CS V G SESVIP P
Sbjct: 302 RLSNISGENIGIAGKLFGIKESPFSSVTLSNVSLSMSSGSSVSWQCSYVYGSSESVIPEP 361

Query: 427 CSDL 431
           C +L
Sbjct: 362 CPEL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881588.19.5e-25898.46probable polygalacturonase isoform X5 [Benincasa hispida][more]
XP_038881575.19.5e-25898.46probable polygalacturonase isoform X4 [Benincasa hispida] >XP_038881581.1 probab... [more]
XP_004134229.19.5e-25898.24probable polygalacturonase isoform X2 [Cucumis sativus] >XP_011651066.1 probable... [more]
XP_008438914.11.6e-25798.02PREDICTED: probable polygalacturonase isoform X2 [Cucumis melo][more]
XP_011651065.12.1e-25798.45probable polygalacturonase isoform X1 [Cucumis sativus] >KAE8650399.1 hypothetic... [more]
Match NameE-valueIdentityDescription
A7PZL33.3e-11247.56Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW072.4e-2527.52Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q8RY292.0e-2426.12Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P432124.9e-2325.86Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
P353363.0e-2023.33Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LAN44.6e-25898.24Pectate_lyase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G16... [more]
A0A1S3AXL37.9e-25898.02probable polygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A1S3AX491.8e-25798.23probable polygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483871 PE... [more]
A0A6J1GUW51.0e-24493.65probable polygalacturonase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
A0A6J1C8711.3e-24493.42probable polygalacturonase OS=Momordica charantia OX=3673 GN=LOC111009229 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G06770.22.7e-16263.82Pectin lyase-like superfamily protein [more]
AT3G16850.11.0e-16162.61Pectin lyase-like superfamily protein [more]
AT5G49215.11.2e-15762.90Pectin lyase-like superfamily protein [more]
AT5G49215.21.6e-15762.84Pectin lyase-like superfamily protein [more]
AT3G06770.34.4e-14465.66Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 358..380
e-value: 4700.0
score: 2.4
coord: 295..316
e-value: 2600.0
score: 4.3
coord: 183..205
e-value: 1700.0
score: 5.6
coord: 267..289
e-value: 780.0
score: 8.0
coord: 324..345
e-value: 6800.0
score: 1.3
coord: 206..232
e-value: 5100.0
score: 2.2
coord: 233..254
e-value: 930.0
score: 7.4
IPR024535Pectate lyase superfamily proteinPFAMPF12708Pectate_lyase_3coord: 39..96
e-value: 1.4E-7
score: 31.6
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 132..416
e-value: 3.5E-33
score: 115.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 23..419
e-value: 9.0E-111
score: 372.8
NoneNo IPR availablePANTHERPTHR31339:SF15PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 2..444
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 2..444
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 34..432

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012531.1HG10012531.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity