HG10012433 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10012433 vs. NCBI nr
Match: XP_038891105.1 (DNA topoisomerase 3-beta isoform X1 [Benincasa hispida])

HSP 1 Score: 1662.9 bits (4305), Expect = 0.0e+00
Identity = 815/869 (93.79%), Postives = 830/869 (95.51%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLG+HAHYKVTSVIGHV
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDFPAGFQDWK+TDPLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFPAGFQDWKSTDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPN+NEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNKNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTF PEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFTPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQ+AMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+L+KN EWGDY
Sbjct: 301 LKVASSALGFGPQIAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLMKNSEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGE+FNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGELFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL PTNLGITL+RG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLAPTNLGITLVRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKY YFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYCYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKGAIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGA SKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           C HPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRIST NE
Sbjct: 721 CLHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTRNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKS FRGRGS  RG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSLFRGRGS--RG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGRGGRGRGRNSG+PMDPKMSFRDF
Sbjct: 841 RGRGRGRGGRGRGRNSGRPMDPKMSFRDF 867

BLAST of HG10012433 vs. NCBI nr
Match: XP_008439059.1 (PREDICTED: DNA topoisomerase 3-beta [Cucumis melo])

HSP 1 Score: 1646.3 bits (4262), Expect = 0.0e+00
Identity = 806/869 (92.75%), Postives = 824/869 (94.82%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWKTT+PLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFV+KID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG  SKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG  GRGRNSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of HG10012433 vs. NCBI nr
Match: KAA0067650.1 (DNA topoisomerase 3-beta [Cucumis melo var. makuwa])

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 808/871 (92.77%), Postives = 826/871 (94.83%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWKTT+PLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFV+KID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG  SKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRG-GR-GRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG GR GRGRNSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of HG10012433 vs. NCBI nr
Match: XP_004147675.1 (DNA topoisomerase 3-beta isoform X1 [Cucumis sativus] >KGN57242.1 hypothetical protein Csa_011650 [Cucumis sativus])

HSP 1 Score: 1642.1 bits (4251), Expect = 0.0e+00
Identity = 803/869 (92.41%), Postives = 825/869 (94.94%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWK+TDPLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVVQ+VLQQF+QKYSYFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG  SKGMGMPCF+
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG  GRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRG--GRGRSSGKPMDPKMSFRDF 867

BLAST of HG10012433 vs. NCBI nr
Match: XP_023528530.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1637.5 bits (4239), Expect = 0.0e+00
Identity = 800/869 (92.06%), Postives = 825/869 (94.94%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDFPA FQDWK TDPLDLFQASVIK ESNPK  +   +        C VL +      
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN+ RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYC  CEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGA SKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGR GRGRGR+SGK MDPKMSFRDF
Sbjct: 841 RGRGRGR-GRGRGRSSGKLMDPKMSFRDF 868

BLAST of HG10012433 vs. ExPASy Swiss-Prot
Match: F4ISQ7 (DNA topoisomerase 3-beta OS=Arabidopsis thaliana OX=3702 GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 635/864 (73.50%), Postives = 727/864 (84.14%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GST+VHEFDG F G+ AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PAGFQDWKTTDPLDLFQASVIKTESNPK-----FLLGPLVCFVLLIRFL-------LVLL 125
           P  +Q+W T DP DLF A +IK ESNPK      L         ++ +L        +  
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 AVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDLKVGV 185
            VIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMFL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+  L
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+C  CEEVYYLPQKG +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +      G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT+ +C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRGRNSGKPMDPKMSFRDF 858
           GRG    GR   K +DPKMSFRDF
Sbjct: 847 GRG----GRRGSKSVDPKMSFRDF 865

BLAST of HG10012433 vs. ExPASy Swiss-Prot
Match: Q0J0S6 (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3B PE=2 SV=1)

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 614/868 (70.74%), Postives = 716/868 (82.49%), Query Frame = 0

Query: 2   VASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHVF 61
           +A TVLMVAEKPSIALSIASALS G+MSTRKGSTDVHEFDG F G HA +KVTSVIGHV 
Sbjct: 1   MAPTVLMVAEKPSIALSIASALSGGRMSTRKGSTDVHEFDGMFQGSHAFFKVTSVIGHVL 60

Query: 62  SIDFPAGFQDWKTTDPLDLFQASVIKTESNPK-----FLLGPLVCFVLLIRFL------- 121
           S+DFP  +Q+W+ TDP+DLF A V+++E NPK      L         L+ +L       
Sbjct: 61  SVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGE 120

Query: 122 LVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDL 181
            +   VI+CTG   +E+ ++++RA+FSSVTEKDI+ A++NLV P+++EALAVDARQEIDL
Sbjct: 121 NICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDL 180

Query: 182 KVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPY 241
           KVGVAFTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+ QITTFKPEKFWS+  Y
Sbjct: 181 KVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTY 240

Query: 242 IMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLL 301
           ++++G E+ L+W+R KLFD DVT MFQK V  DGI +VT+IS K+E K RP GLNTVNLL
Sbjct: 241 VIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLL 300

Query: 302 KVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYV 361
           KVASSALG GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF+  L +L  NP W + V
Sbjct: 301 KVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDV 360

Query: 362 RMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYI 421
           R  L  G+ KP+ G D GDHPPITPM LATE+ LG DAWRLYQY+CQHFIG++S DC+Y 
Sbjct: 361 RTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVSPDCRYT 420

Query: 422 RTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNT 481
           RT I+F+  GE F+C G+RV  KGFTSIMPWLAV+E  +P + KG+ + + +VD+Y+G+T
Sbjct: 421 RTSIEFTSGGETFHCVGNRVTSKGFTSIMPWLAVSENNIPAYKKGDAVSIHKVDIYEGST 480

Query: 482 TPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGY 541
           TPPDYLSESELISLMEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGY
Sbjct: 481 TPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGY 540

Query: 542 QCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALFE 601
           QCID DLCLPDIR FIEQQITL+AKG+ADH  VVQ+VLQQF +KYSYFV+KI++MDALFE
Sbjct: 541 QCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFE 600

Query: 602 AQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCPL 661
           AQFSPLADSGR LSKCGKC RYMK+IS+QP RLYC  CEEVYYLPQ G+IKLYKE+ CPL
Sbjct: 601 AQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPL 660

Query: 662 DNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFLC 721
           D FELL+FS+ GP+ KSFPLCP+CYNSPPFEGID L+GA K +  +G + KG GMPCFLC
Sbjct: 661 DGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLC 720

Query: 722 PHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEK 781
            HPTC+ S+ITQGVCACPEC+GTL+LDPVSAP W+L CN+CNC+V LP  AH+ISTT++K
Sbjct: 721 LHPTCKQSMITQGVCACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKK 780

Query: 782 CADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGR 841
           C  C+STIIEVDFNKKTTPL+DGATL  GCILCDE LHSL+EMKHGKSFF  RG RGRGR
Sbjct: 781 CPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRRG-RGRGR 840

Query: 842 GRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           GRGRGRG     R S +  DPKMSFRDF
Sbjct: 841 GRGRGRGSSRGRRGSSRHDDPKMSFRDF 866

BLAST of HG10012433 vs. ExPASy Swiss-Prot
Match: Q9Z321 (DNA topoisomerase 3-beta-1 OS=Mus musculus OX=10090 GN=Top3b PE=1 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 2.7e-248
Identity = 445/880 (50.57%), Postives = 573/880 (65.11%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTDVHEFDGRFLGYHAHYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G MS+ K   G+  VH++ G F G   H+K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVM 62

Query: 65  SIDFPAGFQDWKTTDPLDLF-QASVIKTESNPKFLL-----------------------G 124
           ++DF   +  W   DP +LF QA   K E+NPK  +                       G
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEG 122

Query: 125 PLVCFVLLIRFLLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNE 184
             +CF +L   L V+           +   K V+RARFSS+T+ DI  A+  L +P+ NE
Sbjct: 123 ENICFEVLDAVLPVM--------NNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNE 182

Query: 185 ALAVDARQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITT 244
           AL+VDARQE+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +
Sbjct: 183 ALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQS 242

Query: 245 FKPEKFWSVCPYIMQNGYE-LNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQEN 304
           FKPE +W +   +  +  E L LDW+R ++FD ++  MF      +  A V   S K++ 
Sbjct: 243 FKPETYWVLQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKA 302

Query: 305 KVRPSGLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTL 364
           K RP  LNTV +L+VASSALG GPQ AMQ+AERLYTQG+ISYPRTE+T YP +FD KG+L
Sbjct: 303 KQRPLALNTVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSL 362

Query: 365 HSLVKNPEWGDYVRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQ 424
                +P W D V+  L++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +
Sbjct: 363 RQQANHPYWADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITR 422

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVN-EKKLPQFLKGE 484
           HFI ++S DCKY+++ I F I  E F C G  VI  GFT IMPW +V  E+ LP   KG+
Sbjct: 423 HFIATVSHDCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGD 482

Query: 485 KIEVARVDLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRR 544
              V  V + +  T+PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRR
Sbjct: 483 TFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRR 542

Query: 545 LVPTNLGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYS 604
           L PTNLGI L+ GY  ID +L LP IRS +E+Q+ L+A+G+AD+  V+ + L  FK+K+ 
Sbjct: 543 LKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFH 602

Query: 605 YFVEKIDHMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQ 664
           YFV+ I  MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+CS C+E Y LPQ
Sbjct: 603 YFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQ 662

Query: 665 KGAIKLYKELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSS 724
            G IKLYKEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF                
Sbjct: 663 NGTIKLYKELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRD-------------- 722

Query: 725 GALSKGMGMPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLV 784
             + KGMG  C  C HPTC+HSL   G+  C EC +G LVLDP S P WK+ CN CN + 
Sbjct: 723 --MKKGMG--CNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVA 782

Query: 785 FLPQGAHRISTTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKH 844
              + AHR+  + + C  C++ +++VDFNK  +PL    T   GCI CD     L+E+KH
Sbjct: 783 HCFENAHRVRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKH 842

Query: 845 GKSFF-RGRGSRGRGRGRGRGRGGRGRGRNSGKPMDPKMS 854
             S     RG  GR +GRGRGRG R  G+ + +    KMS
Sbjct: 843 AASCHPMHRGGPGRRQGRGRGRGRRPPGKPNPRRPKDKMS 855

BLAST of HG10012433 vs. ExPASy Swiss-Prot
Match: O95985 (DNA topoisomerase 3-beta-1 OS=Homo sapiens OX=9606 GN=TOP3B PE=1 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 1.9e-246
Identity = 440/880 (50.00%), Postives = 574/880 (65.23%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRK---GSTDVHEFDGRFLGYHAHYKVTSVIGHVF 64
           TVLMVAEKPS+A SIA  LS G +S+ K   G+  VHE+ G F G    +K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVM 62

Query: 65  SIDFPAGFQDWKTTDPLDLF-QASVIKTESNPKFLL-----------------------G 124
           ++DF   +  W   DP +LF QA   K E+NPK  +                       G
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEG 122

Query: 125 PLVCFVLLIRFLLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNE 184
             +CF +L   L V+         GG    K V+RARFSS+T+ DI  A+  L +P+ NE
Sbjct: 123 ENICFEVLDAVLPVM-----NKAHGG---EKTVFRARFSSITDTDICNAMACLGEPDHNE 182

Query: 185 ALAVDARQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITT 244
           AL+VDARQE+DL++G AFTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +
Sbjct: 183 ALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQS 242

Query: 245 FKPEKFWSVCPYI-MQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQEN 304
           FKPE +W +   +       L LDW+R ++FD ++  MF      +  A+V   S K++ 
Sbjct: 243 FKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKA 302

Query: 305 KVRPSGLNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTL 364
           K RP  LNTV +L+VASS+LG GPQ AMQ AERLYTQG+ISYPRTE+T YP +FD KG+L
Sbjct: 303 KQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSL 362

Query: 365 HSLVKNPEWGDYVRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQ 424
                +P W D V+  L++G ++PR G D GDHPPITPM  ATE  LG DAWRLY+Y+ +
Sbjct: 363 RQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITR 422

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVN-EKKLPQFLKGE 484
           HFI ++S DCKY+++ I F I  E+F C+G  V+  GFT +MPW +V  E+ LP   +G+
Sbjct: 423 HFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGD 482

Query: 485 KIEVARVDLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRR 544
              V  V + +  T PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRR
Sbjct: 483 AFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRR 542

Query: 545 LVPTNLGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYS 604
           L PTNLGI L+ GY  ID +L LP IRS +E+Q+ L+A+G+AD+  V+ + L  FK+K+ 
Sbjct: 543 LKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFH 602

Query: 605 YFVEKIDHMDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQ 664
           YFV+ I  MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+CS C+E Y LPQ
Sbjct: 603 YFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQ 662

Query: 665 KGAIKLYKELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSS 724
            G IKLYKEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF                
Sbjct: 663 NGTIKLYKELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRD-------------- 722

Query: 725 GALSKGMGMPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLV 784
             + KGMG  C  C HP+C+HSL   G+  C EC SG LVLDP S P WK+ CNKCN + 
Sbjct: 723 --MKKGMG--CNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVA 782

Query: 785 FLPQGAHRISTTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKH 844
              + AHR+  + + C+ C++ +++VDFNK  +PL    T  +GC+ CD     L+E+KH
Sbjct: 783 HCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKH 842

Query: 845 GKSFF-RGRGSRGRGRGRGRGRGGRGRGRNSGKPMDPKMS 854
             S     RG  GR +GRGRGR  R  G+ + +    KMS
Sbjct: 843 AASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMS 855

BLAST of HG10012433 vs. ExPASy Swiss-Prot
Match: O96651 (DNA topoisomerase 3-beta OS=Drosophila melanogaster OX=7227 GN=Top3beta PE=2 SV=2)

HSP 1 Score: 763.1 bits (1969), Expect = 3.4e-219
Identity = 399/859 (46.45%), Postives = 540/859 (62.86%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGS---TDVHEFDGRFLGY-HAHYKVTSVIGHV 64
           +VLMVAEKPS+A S+A  LS+G+ + ++G+      HE+ G F      H+++TSV GHV
Sbjct: 3   SVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHV 62

Query: 65  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLVCFVLLIRFLLVLLAVIECTG 124
            S+DF   +  W   DP+ LF  +  K E+NPK  +   +      R    L+  ++C  
Sbjct: 63  MSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHE--ARGCDYLVLWLDCDK 122

Query: 125 FGGN---EIRKKV-------------YRARFSSVTEKDILKALDNLVQPNRNEALAVDAR 184
            G N   E+   V             YRA FS++TEKDI KA++ L  PN NEA +VDAR
Sbjct: 123 EGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDAR 182

Query: 185 QEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFW 244
           QE+DL++G AFTRFQT FFQ +YG+LDS LISYGPCQTPTLGFCV+RH  I TFKPE FW
Sbjct: 183 QELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFW 242

Query: 245 SVCPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLN 304
            +   ++    E+ L+W RG++F  D+  M   RV E   A V  ++ K+  K +P  LN
Sbjct: 243 HL--QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALN 302

Query: 305 TVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPE 364
           TV L+++ SS LG GP  AMQ+AERLYTQG+ISYPRTE+  YP++FD    LH L  + +
Sbjct: 303 TVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSAD 362

Query: 365 WGDYVRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSS 424
           +G+  R  L D    PR G D GDHPPITPM L       RD WR+Y+++C+HF+G++S 
Sbjct: 363 FGEEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSR 422

Query: 425 DCKYIRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAV-NEKKLPQFLKGEKIEVARVD 484
           D KY  T  + S+  E F+CT S +I  GFT +M W A   ++  P F++G ++ +  V 
Sbjct: 423 DLKYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVR 482

Query: 485 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 544
           L +  T PPDYL+ESELI+LME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI
Sbjct: 483 LIESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGI 542

Query: 545 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDH 604
            L+ GYQ IDP+L LP +R+ +E+ +TL+A+G A+   V+++ ++ FK K+ YFV+ ID 
Sbjct: 543 VLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDS 602

Query: 605 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYK 664
           MDALFE  FSPLA+SG+A S+CGKC RYMK+I ++P RL+CS C+E Y LP  G +K+Y+
Sbjct: 603 MDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYR 662

Query: 665 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMG 724
           E  CPLD+F+LL FS  G +G+S+P CPYCYN PPF  +  L G                
Sbjct: 663 EFKCPLDDFDLLAFS-TGVKGRSYPFCPYCYNHPPFSDMPHLGG---------------- 722

Query: 725 MPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHR 784
             C  C +  C HSL T G+ +C EC +G LVLD   AP WKL CN+C+ ++   +GA +
Sbjct: 723 --CNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATK 782

Query: 785 ISTTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGR 842
           I+    KC +C +  + V +    +  +DG+    GCI C      L+E KH     R  
Sbjct: 783 ITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPV 835

BLAST of HG10012433 vs. ExPASy TrEMBL
Match: A0A1S3AYH7 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103483959 PE=3 SV=1)

HSP 1 Score: 1646.3 bits (4262), Expect = 0.0e+00
Identity = 806/869 (92.75%), Postives = 824/869 (94.82%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWKTT+PLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFV+KID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG  SKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG  GRGRNSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of HG10012433 vs. ExPASy TrEMBL
Match: A0A5A7VKD3 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00170 PE=3 SV=1)

HSP 1 Score: 1642.9 bits (4253), Expect = 0.0e+00
Identity = 808/871 (92.77%), Postives = 826/871 (94.83%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVASTVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWKTT+PLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMC ATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVV++VLQQFKQKYSYFV+KID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG  SKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRG-GR-GRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG GR GRGRNSGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of HG10012433 vs. ExPASy TrEMBL
Match: A0A0A0L9C7 (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 1642.1 bits (4251), Expect = 0.0e+00
Identity = 803/869 (92.41%), Postives = 825/869 (94.94%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVA TVLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HA+YKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDF AGFQDWK+TDPLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YI+Q GYELNLDWERGKLFDCDVTAMFQKRV+EDGIAEVTEISEKQENKVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSRV VKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QITLVAKGQADHSSVVQ+VLQQF+QKYSYFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYC ICEEVYYLPQKG IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG  SKGMGMPCF+
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLP+GAHRISTT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGRGRG  GRGR+SGKPMDPKMSFRDF
Sbjct: 841 RGRGRGRG--GRGRSSGKPMDPKMSFRDF 867

BLAST of HG10012433 vs. ExPASy TrEMBL
Match: A0A6J1IRJ0 (DNA topoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111479340 PE=3 SV=1)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 799/869 (91.94%), Postives = 823/869 (94.71%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDFPA FQDWK TDPLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRM LSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYC  CEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGID LYGAAKTNPSSGA SKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGR   GRGRGR+SGK MDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of HG10012433 vs. ExPASy TrEMBL
Match: A0A6J1GWS0 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111457562 PE=3 SV=1)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 798/869 (91.83%), Postives = 824/869 (94.82%), Query Frame = 0

Query: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLG+HAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLV--------CFVLLI----RF 120
           FSIDFPA FQDWK TDPLDLFQASVIKTESNPK  +   +        C VL +      
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 LLVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEID 180
             +   VIECTGFGGNE RKKVYRARFSSVTEKDILKALDNLV+PNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRVMEDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+ LSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480
           +RTKIQFSIAGEIFNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKYSYFV+KIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYC  CEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGA SKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
           RGRGR   GRGRGR+SGK MDPKMSFRDF
Sbjct: 841 RGRGR---GRGRGRSSGKLMDPKMSFRDF 866

BLAST of HG10012433 vs. TAIR 10
Match: AT2G32000.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 635/864 (73.50%), Postives = 727/864 (84.14%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGYHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GST+VHEFDG F G+ AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PAGFQDWKTTDPLDLFQASVIKTESNPK-----FLLGPLVCFVLLIRFL-------LVLL 125
           P  +Q+W T DP DLF A +IK ESNPK      L         ++ +L        +  
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 AVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDLKVGV 185
            VIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 AFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           AF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGLNTVNLLKVAS 305
           GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMFL 365
           SALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+  L
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGNTTPPD 485
           + SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RV+LY+GNT PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYKELTCPLDNFE 665
           PLADSGRALSKCGKCLRYMK I++ P RL+C  CEEVYYLPQKG +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +      G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTNEKCADC 785
           C+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI+TT+ +C +C
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGGRGRGRNSGKPMDPKMSFRDF 858
           GRG    GR   K +DPKMSFRDF
Sbjct: 847 GRG----GRRGSKSVDPKMSFRDF 865

BLAST of HG10012433 vs. TAIR 10
Match: AT2G32000.2 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 588/814 (72.24%), Postives = 677/814 (83.17%), Query Frame = 0

Query: 59  HVFSI---DFPAGFQDWKTTDPLDLFQASVIKTESNPK-----FLLGPLVCFVLLIRFL- 118
           H+ S+   DFP  +Q+W T DP DLF A +IK ESNPK      L         ++ +L 
Sbjct: 30  HLLSVMFSDFPEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLD 89

Query: 119 ------LVLLAVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNRNEALAVDA 178
                  +   VIE TGF   + ++KVYRARFSSVTEKDI KA+DNLV+PNR+EALAVDA
Sbjct: 90  CDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDA 149

Query: 179 RQEIDLKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 238
           RQEIDLKVGVAF+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKF
Sbjct: 150 RQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKF 209

Query: 239 WSVCPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQENKVRPSGL 298
           W++ PYI ++GYEL L+WER +LFD +   +FQK V+E   A+V ++SEKQE K RP+GL
Sbjct: 210 WALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGL 269

Query: 299 NTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNP 358
           NTVNLLKVASSALGFGPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP
Sbjct: 270 NTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNP 329

Query: 359 EWGDYVRMFLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLS 418
            WG YV+  LSDG+  P+SGTD GDHPPITPM  ATE M+G DAWRLYQYVCQHF+G++S
Sbjct: 330 VWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVS 389

Query: 419 SDCKYIRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVD 478
            +CKYIRTK++ SI GE F+CTG RV  KGFT+IMPW AV+EKKLP FLKGE+IEV RV+
Sbjct: 390 PNCKYIRTKVELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVE 449

Query: 479 LYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 538
           LY+GNT PPDYL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGI
Sbjct: 450 LYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGI 509

Query: 539 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYSYFVEKIDH 598
           TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHS VVQ+V+QQF++K+SYFV++I+H
Sbjct: 510 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEH 569

Query: 599 MDALFEAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCSICEEVYYLPQKGAIKLYK 658
           MDALFEAQFSPLADSGRALSKCGKCLRYMK I++ P RL+C  CEEVYYLPQKG +KLYK
Sbjct: 570 MDALFEAQFSPLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYK 629

Query: 659 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGALSKGMG 718
           ELTCPLDNFEL+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +      G G
Sbjct: 630 ELTCPLDNFELVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAG 689

Query: 719 MPCFLCPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRI 778
           MPC LCPHPTC+HS+  QGVCACPEC GTLVLDPVS P WKLNCN C+C+V LP+GAHRI
Sbjct: 690 MPCSLCPHPTCQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRI 749

Query: 779 STTNEKCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFFRGRG 838
           +TT+ +C +CDS IIE+DFNKKTTPLE+GATL  GC+LCDE L SL+E+KHG+SF R RG
Sbjct: 750 TTTSNRCPECDSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RG 809

Query: 839 SRGRGRGRGRGRGGRGRGRNSGKPMDPKMSFRDF 858
            RGRGRGRGRGRG    GR   K +DPKMSFRDF
Sbjct: 810 GRGRGRGRGRGRG----GRRGSKSVDPKMSFRDF 838

BLAST of HG10012433 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 338.6 bits (867), Expect = 1.5e-92
Identity = 204/594 (34.34%), Postives = 313/594 (52.69%), Query Frame = 0

Query: 5   TVLMVAEKPSIALSIASALSHGQMSTRKGST---DVHEFDGRFLGYHAHYKVTSVIGHVF 64
           TVL VAEKPS+A S+A  LS G   TR+G +    + EFD    G      +TSVIGH+ 
Sbjct: 10  TVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLM 69

Query: 65  SIDFPAGFQDWKTTDPLDLFQASVIKTESNPKFLLGPLVCFVLLIRFLLVLLAVIECTGF 124
            ++F   ++ W + DP DL+QA V+K     K  +   +         LVL   ++C   
Sbjct: 70  ELEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLW--LDCDRE 129

Query: 125 GGN----------EIRKKVY--RARFSSVTEKDILKALDNLVQPNRNEALAVDARQEIDL 184
           G N           ++  ++  RA FS++ ++DI +A+ NL  PN+  A AVDARQEIDL
Sbjct: 130 GENIAFEVVDVCRAVKHNLFIRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEIDL 189

Query: 185 KVGVAFTRFQTTFFQGKY-----GNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFW 244
           ++G +FTRFQT   + ++     G   SR+ISYGPCQ PTLGF V+R+ +I   +PE+FW
Sbjct: 190 RIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEFW 249

Query: 245 SV-CPYIMQNGYELNLDWERGKLFDCDVTAMFQKRVMEDGIAEVTEISEKQEN-KVRPSG 304
           ++ C +  + G     +W RG LFD     +  +  +E+  A V  +   +E  K  P  
Sbjct: 250 TINCSHQSEEGL-ATFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPYP 309

Query: 305 LNTVNLLKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKN 364
           LNT+ L K AS       +  M++AE LY  GFISYPRTE+ ++ S  D +  +    ++
Sbjct: 310 LNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTRH 369

Query: 365 PEWGDYVRMFLSD----GYSKPRSGTDVGDHPPITPMCLAT-EDMLGRDAWRLYQYVCQH 424
           P WG Y +  L        +    G D   HPPI P   ++ E    RD   +Y+ V +H
Sbjct: 370 PAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVRH 429

Query: 425 FIGSLSSDCKYIRTKIQFSIAGEIFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKI 484
           ++  +S       T ++  IAGE F+ +G  ++ K +  +  + +     +P + KG++ 
Sbjct: 430 YLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQF 489

Query: 485 EVARVDLYQGNTTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLV 544
               + L    T PP  L E++L+S M+K GIGTDA++  HI  + +R Y    A  R  
Sbjct: 490 IPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRFS 549

Query: 545 PTNLGITLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFK 572
           PTNLG  L+ GY  +  +L  P++R+ +E  +  V+ G+   + V++  LQQ K
Sbjct: 550 PTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMK 600