HG10012295 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10012295
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Prolyl-tRNA synthetase
Location	Chr01: 19819100 .. 19823067 (-)
RNA-Seq Expression	HG10012295
Synteny	HG10012295

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGTTTCGCTGAGACTACCTTTGATCTCCTCTTTGCTTTCCCCAGCCTCATCTCGCCCCTCAAACTTCGCCTTCTCCAGGCGAACTTTGGGGATTTCATGGAGGCAGCAACGCCCGTTACGGTTGCCGCTGCTTTCTTCTGGTTTCTCTACTCGAAGTTCATCCACCGGGACCGAAATTCGTGTCGACAACACGAAGAATCGAGTTCCGGAAGGAGCTATCACGCCTCGATCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCTAATGCCGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTACGCCATATGGGAGGCAATACAGGTGGCGGAAGCTATAATTTATCTTTACTTTGCCTGCAACATTCGAACGCTGTGTTTTGAGCTTTTCTTGTATGTTTAATCAGACCATGATTCTTTCTGCAGGTATATTTAAATGCCAAGTTCAAGGAAACCGGCCATGAGAATATGTACTTTCCGCAGGTGTGTCTTTGGCGTGTTTATGAGTGGATATGAATGAGCGTATTTGCTGGAAATGTAAAATGATGAAATCTTTAATTTTGACTCCAGTTTATACCATACTCTTTTATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCAGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTACGTAGACTAAGCAAAGCCATTTGCCCTTTTCCCGTCCTTCATTTCTCATTAGAAATATTAACCTTATTGTATGTGGTACTTTGCATTGGCAAAATTGGAAATGGATATTAGCCCTCAATAACAGGGGACCTGCGGTTTGGTTTTGATTGTTGGTTTGCCTGAGGCTTTGGCAGGTTCGACCGACAAGTGAAACCATTGTGAATCACATGTTTACTCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATCAATCAGGTTCATTGACTTGCCATATTATGAAAAATTCTTCCTTTGTCGTTGGCTTTTATGTTGTCCTTATTAGATCTTTTCTTTTGTGTATTTGATGTAGTGGGCCAATGTTACTAGATGGGAGATGCGCACGAAACCATTTGTTAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACAGCTCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGTATATTTGATGCATCCAAGATTACTTTGTGCTCTTTTTTTAATAAGCATATCCTTTTCATTTAAGGACTTTGATGCTGCATTGCAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAGTCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCTATGATGGGGGATCGAAAGGCACTGCAGGCAGGAACCAGCCACAACCTAGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGGTACTTTGGCAAATCAATGAAATTTCTTCGAATGAATTTTACAATATATGAGATATATTCTTAATTCCATTATTGTAGCACATGCACGATTCTTGTTCCTATCGCACTTTGGTGTCAAACTGATATTCAAATTCTTCTTGATTAGTTAATATGGTTCATGAATTCCCTTTTGAGACATTCAACCATAACATTATTTGATGTCTGACATATTAAATTGCACTTTCGATGCGACTCGGGTTACTGAAACATTTAATGGAGTAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCGTGGGCAGTTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCCATACAGGTATTGATATGAATTTGTAATTTGTACAGTATTACTATCTCTGAATTCTATAAACTATTTCTGATGACAATTACCTAAAGTTAGTCAGGATGTAAAAGTGACCAGAGATTCTGTGCACATAAGATACTTGTCTTTTGTCCAACTCTTTAAAACTTACGATTTGTAGGAGAACATCTGATAATGGCAACACTTTCTGTTTTTTGAGCCGCTTGATTCATATTTTTCCTTTCTTTTTAACATGGCTTACTTATATCCAGGTGGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCTGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGAAAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGTTGGTGCTTATATTTTATGATTAAGATCAATCTAATAAAAAGAATGATTAAGATCAAGCTCTGACCTTTGTCTTCCCCTCAGTTTAGGAAAAGTTTGTCTAAAAACGAACATTAACTTTAAATTTTCAGCATATGTTGACTGGTTAGCTGCCAATAGATATTCATGTTGACTCTTCTATGATTTCTGTATCTTTTATGGGCAACTTGGAATCGCGTTTGTGAGAAGAAAATACTTCTGCAGTGTAGGTTGGCTGGCTTGCATTTTTATTACAGGATGTATGCATATTGGTTATTGATAGTTTTGAATATTAAATTCCCCTTTTTAGCGACATGATTTTTGCCTAGATTTTGTCTTCCTCCCTCTTTGCATTTGTTGCTATTAATTTGAAATCAAAGATTTGTAACAAGGCTTTGATTGTAGGGCGTTCCTTTGAGGATTGAGATTGGGCCCAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGAATATCCATGGAACCGTCTCTTTTAGAAGCTTATGTAAAAGGGACATTGGATGAGATCCAGTCATCTCTTCTTCAACAGGCAAAAACTTTTCGTGACAGGTACGCCCTTATTTTCTTATGTTGGGATGTTATTTGATAAATATGTAAATCTGCATTAAGTTACATTACCTTTATTTTATTATTATTTTTTCAATGTTCGAGCCAGCTTACGTGCACCTCGACTAATCTCACGGGACAACGTGCCCGACCTTACAACATTTGGTTGTCAAAGAAACCCGTAGGATATTAAATCCTAAGAAAGTGGTCACCATGAATCGAATCCATGACCCCTTAGTCCTTTAACCTTTTTTGATGTTCCCACTACCACTAGGCTAACCCGTGATGGTTCATTACCTTTATTCTTTTAGAATTGAAGTAATAGAATGTTGTAGAATGCTTCTTGCAAATCGTAGTACAATGTTAGATTCTTAGCCATTAGAATGTATTACAGTATTTGTAATACATTCTATTGAATTGTGAGCTTGGAGAAAATTGTTTCCATCTTCACCCTCAATTTCGTCCTGTCCCTGCACCTTTGCTTCCCCATAAAATTGTTTCCACCTGGTTCATGTATGAAAAATTCTCTAAATGTATCATTATATCTAAAAGGGAAAGAAGGGATGGGGAATGAATGGGTGTGCTTTGCAGTTCTCATTTCATTGGCTTCCGTGGTAGTCATATTCTTGATCAACATTCCATTACTTCATATTCTCATTTCATCAGCTTCCATGGTAGTCATATTTTGTGGCAGTCGCATTTCATCAGCTGCCATCAGATGCCGATTCCAATCTTTACCTATCCAGCTCCTTTACCAACCCCACGTGACTATCCTAATCCTTATTCCTATGATGTGCCTCTTGATGCACATTGGTTGACTTCAATCCCCCACATATGGACGAAGTGTTCAATCCAGGTCCTCACATACTTGCTTTAGCTCCATGGTTCAGAGAAAATGACCTTTGTATTATGGCCATTTCTACAAGAACAAAGAAGAACAAGCAGTCGGTGAAACAAAAGAAAAGCATCAGAGAGATCTGGAATTTAGAGTGCTCAATCTCATATGAGAAAAAAGCATCATCCATCCTTCAAGAGAGCTCATTGTCGGCTTGA

mRNA sequence

ATGGTAGTTTCGCTGAGACTACCTTTGATCTCCTCTTTGCTTTCCCCAGCCTCATCTCGCCCCTCAAACTTCGCCTTCTCCAGGCGAACTTTGGGGATTTCATGGAGGCAGCAACGCCCGTTACGGTTGCCGCTGCTTTCTTCTGGTTTCTCTACTCGAAGTTCATCCACCGGGACCGAAATTCGTGTCGACAACACGAAGAATCGAGTTCCGGAAGGAGCTATCACGCCTCGATCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCTAATGCCGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTACGCCATATGGGAGGCAATACAGGTATATTTAAATGCCAAGTTCAAGGAAACCGGCCATGAGAATATGTACTTTCCGCAGTTTATACCATACTCTTTTATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCAGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTTCGACCGACAAGTGAAACCATTGTGAATCACATGTTTACTCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATCAATCAGTGGGCCAATGTTACTAGATGGGAGATGCGCACGAAACCATTTGTTAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACAGCTCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAGTCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCTATGATGGGGGATCGAAAGGCACTGCAGGCAGGAACCAGCCACAACCTAGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCGTGGGCAGTTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCCATACAGGTGGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCTGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGAAAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGGCGTTCCTTTGAGGATTGAGATTGGGCCCAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGAATATCCATGGAACCGTCTCTTTTAGAAGCTTATGTAAAAGGGACATTGGATGAGATCCAGTCATCTCTTCTTCAACAGGCAAAAACTTTTCGTGACAGAACAAAGAAGAACAAGCAGTCGGTGAAACAAAAGAAAAGCATCAGAGAGATCTGGAATTTAGAGTGCTCAATCTCATATGAGAAAAAAGCATCATCCATCCTTCAAGAGAGCTCATTGTCGGCTTGA

Coding sequence (CDS)

ATGGTAGTTTCGCTGAGACTACCTTTGATCTCCTCTTTGCTTTCCCCAGCCTCATCTCGCCCCTCAAACTTCGCCTTCTCCAGGCGAACTTTGGGGATTTCATGGAGGCAGCAACGCCCGTTACGGTTGCCGCTGCTTTCTTCTGGTTTCTCTACTCGAAGTTCATCCACCGGGACCGAAATTCGTGTCGACAACACGAAGAATCGAGTTCCGGAAGGAGCTATCACGCCTCGATCGCAGGACTTCAATGCTTGGTATTTGGATGTTATTGCTAATGCCGAGCTCGCGGATTATGGTCCTGTTCGAGGTACCATGGTTATTCGTCCCTATGGCTACGCCATATGGGAGGCAATACAGGTATATTTAAATGCCAAGTTCAAGGAAACCGGCCATGAGAATATGTACTTTCCGCAGTTTATACCATACTCTTTTATTGAGAAAGAAGCTAGTCATGTCGAGGGGTTTAGTCCAGAGTTAGCTCTTGTAACAATTGGAGGAGGGAAAGAACTTGAAGAAAAGCTTGTGGTTCGACCGACAAGTGAAACCATTGTGAATCACATGTTTACTCAGTGGATTCATAGTTACCGTGATCTTCCTTTGATGATCAATCAGTGGGCCAATGTTACTAGATGGGAGATGCGCACGAAACCATTTGTTAGAACTCTTGAATTTCTCTGGCAGGAGGGTCATACAGCTCATGCCAGTCCAGAAGAGGCAGAAAAAGAGGCTATTCAGATGATTGACATATATACAAAATTTTCTTATGAGCAAGCGGCAATACCTGTTATTGCAGGTCGAAAGTCAAAGGTGGAGACATTTGCTGGTGCAGACAAGACCTATACAATAGAGGCTATGATGGGGGATCGAAAGGCACTGCAGGCAGGAACCAGCCACAACCTAGGGCAGAACTTTTCTCGTGCTTTTGGAACACAGTTCACAGATGAAAATGGACAAAGGCAACATGTATGGCAGACATCGTGGGCAGTTAGTACGCGTTTTGTTGGAGGAATTATTATGACTCATGGAGATGATGGAGGTTTAATGCTTCCACCAAAGCTTGCTCCCATACAGGTGGTAATCATACCCATCTGGAAGAAGGATGGTGATAAAGCTGAAGTTCTTAGTGCTGCTTCTTCTGTAAAAGAAAGTCTTCAAACTGCAGGGATAAAGGTTAAACTTGATGAGTCAGAACAGAGAACTCCAGGATGGAAATTCAATTTCTGGGAGATGAAGGGCGTTCCTTTGAGGATTGAGATTGGGCCCAAGGATGTTGCAAGTCAAAGTGTGGTCATATCTAGGAGAGACATTCCTGGAAAGCAAGGGAAAGTTTTTGGAATATCCATGGAACCGTCTCTTTTAGAAGCTTATGTAAAAGGGACATTGGATGAGATCCAGTCATCTCTTCTTCAACAGGCAAAAACTTTTCGTGACAGAACAAAGAAGAACAAGCAGTCGGTGAAACAAAAGAAAAGCATCAGAGAGATCTGGAATTTAGAGTGCTCAATCTCATATGAGAAAAAAGCATCATCCATCCTTCAAGAGAGCTCATTGTCGGCTTGA

Protein sequence

MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTEIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRTKKNKQSVKQKKSIREIWNLECSISYEKKASSILQESSLSA

Homology

[Close]

BLAST of HG10012295 vs. NCBI nr
Match: XP_038878181.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 936.0 bits (2418), Expect = 1.4e-268
Identity = 469/504 (93.06%), Postives = 481/504 (95.44%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLPLISSLLSPASSR SNF FSRR LGISWRQQRPLRLPLLSSGFS RSSSTGTE
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNFTFSRRALGISWRQQRPLRLPLLSSGFSARSSSTGTE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           IRVDN+KNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV
Sbjct: 61  IRVDNSKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           +LNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 FLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMID+YTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDVYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           IPIWKKDGDK EVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 IPIWKKDGDKVEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           PKDVASQSVVISRRDIPGKQGKVFGISMEPS+LEAYVKGTLDEIQSSLLQ AKTFRD   
Sbjct: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSVLEAYVKGTLDEIQSSLLQLAKTFRDSNI 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ + K  R  W+
Sbjct: 481 VDVSSYDELKEAISEGKWARGPWS 504

[Close]

BLAST of HG10012295 vs. NCBI nr
Match: KAA0039089.1 (proline--tRNA ligase [Cucumis melo var. makuwa] >TYK14878.1 proline--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 904.4 bits (2336), Expect = 4.4e-259
Identity = 452/481 (93.97%), Postives = 464/481 (96.47%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQR-PLRLPLLSSGFSTRSSSTGT 60
           MVVSLRLPLISSLLSPASSR  NFAFSRR LGISWR QR PLRLPLLSS FS RSSST T
Sbjct: 1   MVVSLRLPLISSLLSPASSRSPNFAFSRRALGISWRHQRPPLRLPLLSSPFSPRSSSTNT 60

Query: 61  EIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120
           EIR +++KNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ
Sbjct: 61  EIRTESSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120

Query: 121 VYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180
            YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT
Sbjct: 121 EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180

Query: 181 SETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240
           SETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA
Sbjct: 181 SETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240

Query: 241 EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300
           EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN
Sbjct: 241 EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300

Query: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360
           LGQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQVV
Sbjct: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360

Query: 361 IIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEI 420
           IIPIWKKDGDKAEVL+AASSVKE LQTAG+KVKLDES+Q+T GWKFNFWEMKGVPLRIEI
Sbjct: 361 IIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMKGVPLRIEI 420

Query: 421 GPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT 480
           GPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLLQQA+TFRDR 
Sbjct: 421 GPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVKETLDEIQSSLLQQARTFRDRR 480

[Close]

BLAST of HG10012295 vs. NCBI nr
Match: XP_004149956.3 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_031738676.1 proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN57582.2 hypothetical protein Csa_011595 [Cucumis sativus])

HSP 1 Score: 894.8 bits (2311), Expect = 3.5e-256
Identity = 452/507 (89.15%), Postives = 470/507 (92.70%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQR---PLRLPLLSSGFSTRSSST 60
           MVVSLRLPLISSLLSPASSR SN AFSRR LGISWR QR   PLR PLLSS FS RSSST
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNLAFSRRALGISWRHQRQRPPLRFPLLSSPFSPRSSST 60

Query: 61  GTEIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120
            TEI +D++KNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA
Sbjct: 61  NTEIHIDSSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120

Query: 121 IQVYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180
           IQ YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR
Sbjct: 121 IQEYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180

Query: 181 PTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240
           PTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE
Sbjct: 181 PTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240

Query: 241 EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300
           EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS
Sbjct: 241 EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300

Query: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360
           HNLGQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQ
Sbjct: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360

Query: 361 VVIIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRI 420
           VVIIPIWKKDGDKAEVL+AASSVKE LQTAG+KVKLDES+Q+T GWKFNFWEMKGVPLRI
Sbjct: 361 VVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMKGVPLRI 420

Query: 421 EIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRD 480
           EIGPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK  LDEIQSSLLQQA+TFRD
Sbjct: 421 EIGPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVKERLDEIQSSLLQQARTFRD 480

Query: 481 RT-------KKNKQSVKQKKSIREIWN 498
                     + K+++ + K  R  W+
Sbjct: 481 SNIVDVSSYNELKEAISEGKWARGPWS 507

[Close]

BLAST of HG10012295 vs. NCBI nr
Match: XP_023541480.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 892.1 bits (2304), Expect = 2.3e-255
Identity = 447/504 (88.69%), Postives = 469/504 (93.06%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLP +SSLLSPAS     F FSRR LGISWRQQRP RLPLL+ GFS RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGFSVRSSSTGTE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDN+KNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPP+LAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           IPIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD SEQRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 IPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           PKDVA+QSVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLL+QAKTFRD   
Sbjct: 421 PKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDEIQSSLLEQAKTFRDSNV 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ Q K  R  W+
Sbjct: 481 VDVSSYNELKEAISQGKWARGPWS 500

[Close]

BLAST of HG10012295 vs. NCBI nr
Match: XP_022945172.1 (proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 890.2 bits (2299), Expect = 8.6e-255
Identity = 445/504 (88.29%), Postives = 469/504 (93.06%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLP +SSLLSPAS     F FSRR LGISWRQQRP RLPLL+ G S RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDN+KNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPP+LAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           +PIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD+SEQRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 VPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           PKDVA+QSVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLL+QAKTFRD   
Sbjct: 421 PKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDEIQSSLLEQAKTFRDSNV 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ Q K  R  W+
Sbjct: 481 VDVSSYNELKEAISQGKWARGPWS 500

[Close]

BLAST of HG10012295 vs. ExPASy Swiss-Prot
Match: Q9FYR6 (Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA6 PE=2 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.2e-220
Identity = 381/475 (80.21%), Postives = 421/475 (88.63%), Query Frame = 0


Query: 4   SLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGT-EIR 63
           SLRLP ++SLL PA++R    A  RRT+ +   + RPL      SGF+T  S T + E +
Sbjct: 5   SLRLPSLTSLLFPATTRYP--ATLRRTVCL---RNRPL------SGFATAPSGTASPETK 64

Query: 64  VDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYL 123
                    + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ YL
Sbjct: 65  SSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDYL 124

Query: 124 NAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSET 183
           N KFKETGH NMYFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLVVRPTSET
Sbjct: 125 NVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSET 184

Query: 184 IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKE 243
           IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHA+PEEAEKE
Sbjct: 185 IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHATPEEAEKE 244

Query: 244 AIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQ 303
           A QMI+IYT+F++EQ AIPVI GRKSK+ETFAGAD TYTIEAMMGDRKALQAGTSHNLGQ
Sbjct: 245 AKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDRKALQAGTSHNLGQ 304

Query: 304 NFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIP 363
           NFSRAFGTQF DENG+RQHVWQTSWAVSTRFVGGIIMTHGDD GLMLPPK+APIQVVI+P
Sbjct: 305 NFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVP 364

Query: 364 IWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPK 423
           IWKKD +K  VLSAASSVKE+LQTAG++VKLD+++QRTPGWKFNFWEMKG+PLRIEIGP+
Sbjct: 365 IWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPR 424

Query: 424 DVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRD 478
           DV+S SVV+SRRD+PGK GKVFGISMEPS L AYVK  LDEIQ+SLL++A +FRD
Sbjct: 425 DVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDEIQTSLLEKALSFRD 468

[Close]

BLAST of HG10012295 vs. ExPASy Swiss-Prot
Match: Q5SM28 (Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=proS PE=1 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 7.1e-135
Identity = 245/446 (54.93%), Postives = 323/446 (72.42%), Query Frame = 0


Query: 72  EGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYLNAKFKETGH 131
           E  +TP+SQDF+ WYL+VI  AELADYGPVRGT+V+RPYGYAIWE IQ  L+  FKETGH
Sbjct: 4   EKGLTPQSQDFSEWYLEVIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGH 63

Query: 132 ENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQW 191
           +N YFP FIP SF+ KEA HVEGFSPELA+VT  GG+ELEE L VRPTSET++ +M+++W
Sbjct: 64  QNAYFPLFIPMSFLRKEAEHVEGFSPELAVVTHAGGEELEEPLAVRPTSETVIGYMWSKW 123

Query: 192 IHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYT 251
           I S+RDLP ++NQW NV RWEMRT+PF+RT EFLWQEGHTAHA+ EEAE+E  +M+ IY 
Sbjct: 124 IRSWRDLPQLLNQWGNVVRWEMRTRPFLRTSEFLWQEGHTAHATREEAEEEVRRMLSIYA 183

Query: 252 KFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQ 311
           + + E AAIPVI G K++ E FAGA  T TIEA+M D KALQAGTSH LG+NF+RAF  +
Sbjct: 184 RLAREYAAIPVIEGLKTEKEKFAGAVYTTTIEALMKDGKALQAGTSHYLGENFARAFDIK 243

Query: 312 FTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKA 371
           F D + Q ++V  TSW +S RF+G IIMTHGDD GL+LPP+LAPIQVVI+PI+ KD  + 
Sbjct: 244 FQDRDLQVKYVHTTSWGLSWRFIGAIIMTHGDDRGLVLPPRLAPIQVVIVPIY-KDESRE 303

Query: 372 EVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVI 431
            VL AA  ++++L   G++V LD+ +Q TPG+KF+ WE+KGVP R+E+GPKD+     V+
Sbjct: 304 RVLEAAQGLRQALLAQGLRVHLDDRDQHTPGYKFHEWELKGVPFRVELGPKDLEGGQAVL 363

Query: 432 SRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFR-DRTKKNKQSVKQKK 491
           + R + GK+      ++  + L   + G LD     L ++A  FR D T+K       K+
Sbjct: 364 ASR-LGGKE------TLPLAALPEALPGKLDAFHEELYRRALAFREDHTRKVDTYEAFKE 423

Query: 492 SIREIWNLECSISYEKKASSILQESS 517
           +++E + L      +K    ++QE +
Sbjct: 424 AVQEGFALAFHCG-DKACERLIQEET 440

[Close]

BLAST of HG10012295 vs. ExPASy Swiss-Prot
Match: Q72GF9 (Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=proS PE=3 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.5e-134
Identity = 244/446 (54.71%), Postives = 323/446 (72.42%), Query Frame = 0


Query: 72  EGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYLNAKFKETGH 131
           E  +TP+SQDF+ WYL+VI  AELADYGPVRGT+V+RPYGYAIWE IQ  L+  FKETGH
Sbjct: 4   EKGLTPQSQDFSEWYLEVIQKAELADYGPVRGTIVVRPYGYAIWENIQQVLDRMFKETGH 63

Query: 132 ENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQW 191
           +N YFP FIP SF+ KEA HVEGFSPELA+VT  GG+ELEE L VRPTSET++ +M+++W
Sbjct: 64  QNAYFPLFIPMSFLRKEAEHVEGFSPELAVVTHAGGEELEEPLAVRPTSETVIGYMWSKW 123

Query: 192 IHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYT 251
           I S+RDLP ++NQW NV RWEMRT+PF+RT EFLWQEGHTAHA+ EEAE+E  +M+ IY 
Sbjct: 124 IRSWRDLPQLLNQWGNVVRWEMRTRPFLRTSEFLWQEGHTAHATREEAEEEVRRMLSIYA 183

Query: 252 KFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQ 311
           + + E AAIPVI G K++ E FAGA  T TIEA+M D KALQAGTSH LG+NF+RAF  +
Sbjct: 184 RLAREYAAIPVIEGLKTEKEKFAGAVYTTTIEALMKDGKALQAGTSHYLGENFARAFDIK 243

Query: 312 FTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKA 371
           F D + Q ++V  TSW +S RF+G IIMTHGDD GL+LPP+LAPIQVVI+PI+ KD  + 
Sbjct: 244 FQDRDLQVKYVHTTSWGLSWRFIGAIIMTHGDDRGLVLPPRLAPIQVVIVPIY-KDESRE 303

Query: 372 EVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVI 431
            VL AA  ++++LQ  G++V LD+ +Q TPG+KF+ WE+KGVP R+E+GPKD+     V+
Sbjct: 304 RVLEAAQGLRQALQAKGLRVHLDDRDQHTPGYKFHEWELKGVPFRVELGPKDLEQGQAVL 363

Query: 432 SRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRD-RTKKNKQSVKQKK 491
           + R + GK+      ++  + L   + G L      L ++A  FR+  T+K       K+
Sbjct: 364 ASR-LGGKE------TLPLAALPEALPGKLVAFHEELYRRALAFREAHTRKVDTYEAFKE 423

Query: 492 SIREIWNLECSISYEKKASSILQESS 517
           +++E + L      +K    ++QE +
Sbjct: 424 AVQEGFALAFHCG-DKACERLIQEET 440

[Close]

BLAST of HG10012295 vs. ExPASy Swiss-Prot
Match: B3QPX3 (Proline--tRNA ligase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=517417 GN=proS PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.5e-132
Identity = 233/402 (57.96%), Postives = 293/402 (72.89%), Query Frame = 0


Query: 75  ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYLNAKFKETGHENM 134
           IT R QD++ WY+D++ +A+LADY  VRG MVIRP GYAIWE +Q  L+  FKETGH N 
Sbjct: 5   ITSRQQDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKETGHVNA 64

Query: 135 YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHS 194
           YFP FIP SFI KEA H+EGF+PE A+VT GGG+EL EKL VRPTSETI+   + +WI S
Sbjct: 65  YFPMFIPESFIAKEAEHIEGFAPECAVVTHGGGEELAEKLYVRPTSETIIWSSYKKWIQS 124

Query: 195 YRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFS 254
           YRDLPL+INQWANV RWEMRT+ F+RT EFLWQEGHTAHA PEEA++E I+MI++Y  F+
Sbjct: 125 YRDLPLLINQWANVVRWEMRTRLFLRTTEFLWQEGHTAHADPEEAQEEVIRMINVYRTFA 184

Query: 255 YEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTD 314
            E  A+PVI G+KS+ E FAGAD TY IEAMM D KALQAGTSHNLGQNF++AF  QF  
Sbjct: 185 EEYMAMPVIVGKKSESEKFAGADATYCIEAMMQDGKALQAGTSHNLGQNFAKAFDCQFQT 244

Query: 315 ENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVL 374
           + G   +VW TSW VSTR +G +IM H DD GL+LPPKLA  QVVIIPI K  GDK EV 
Sbjct: 245 KEGVLDYVWATSWGVSTRLIGALIMAHSDDKGLVLPPKLASRQVVIIPILK--GDKEEVR 304

Query: 375 SAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRR 434
           + A  + ++L   GI   +D+SE  +PGWKF  +E++G+P+RIE+GP+D+A    +++RR
Sbjct: 305 AHARFIAKALNKHGISTFVDDSENNSPGWKFAEYELQGIPVRIELGPRDIAEGKCIVARR 364

Query: 435 DIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFR 477
           D   K   +    M  ++ E      L+ IQ +L ++A  FR
Sbjct: 365 DTFEKTEVLLDAEMTENIEE-----ILNNIQQNLYERALQFR 399

[Close]

BLAST of HG10012295 vs. ExPASy Swiss-Prot
Match: B3EP23 (Proline--tRNA ligase OS=Chlorobium phaeobacteroides (strain BS1) OX=331678 GN=proS PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.5e-132
Identity = 230/416 (55.29%), Postives = 304/416 (73.08%), Query Frame = 0


Query: 75  ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYLNAKFKETGHENM 134
           ITPR +D++ WY+D++ +A+LADY  VRG MVIRP GYAIWE +Q  L+  FK TGH N 
Sbjct: 5   ITPREEDYSQWYIDLVRSAKLADYSDVRGCMVIRPNGYAIWEKMQAALDGMFKATGHVNA 64

Query: 135 YFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSETIVNHMFTQWIHS 194
           YFP FIP S+I+KEA H+EGF+PE A+VT GGG+EL EKL VRPTSET++   + +WI S
Sbjct: 65  YFPLFIPESYIQKEADHIEGFAPECAVVTHGGGEELAEKLYVRPTSETVIWASYKKWIQS 124

Query: 195 YRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKEAIQMIDIYTKFS 254
           YRDLPL+INQWANV RWEMRT+ F+RT EFLWQEGHTAHA+PEEA+ E ++MI++Y  F+
Sbjct: 125 YRDLPLLINQWANVVRWEMRTRLFLRTSEFLWQEGHTAHATPEEAQDEVLRMINVYKTFA 184

Query: 255 YEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQNFSRAFGTQFTD 314
            E  A+PV+ GRK+  E FAGAD+T+ IEAMM D+KALQAGTSHNLGQNF++AF  QF  
Sbjct: 185 EEYMAMPVLIGRKTDSEKFAGADETWCIEAMMQDKKALQAGTSHNLGQNFAKAFDCQFQS 244

Query: 315 ENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIPIWKKDGDKAEVL 374
           +NG  ++VW TSW VSTR +G +IM H DD GL+LPPKLA  QVVI+PI +  GDK  V 
Sbjct: 245 KNGTLEYVWATSWGVSTRLIGALIMAHSDDRGLVLPPKLASRQVVIVPILR--GDKQAVA 304

Query: 375 SAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRR 434
             A ++ + L + GI   +D+SEQ +PGWKF  +E++G+P+R+EIGP+D++S   + +RR
Sbjct: 305 EKAHALADELNSNGIHAFVDDSEQNSPGWKFAEYELQGIPVRLEIGPRDISSNICIAARR 364

Query: 435 DIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRTKKNKQSVKQKK 491
           D   KQ     I+++ +   A +   LD IQ S+ + A  FR+   +   S  + K
Sbjct: 365 DTGEKQ----QIALDDT-FAASISSLLDAIQQSMFKSALEFREANTREVSSYDEFK 413

[Close]

BLAST of HG10012295 vs. ExPASy TrEMBL
Match: A0A5D3CUH7 (Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2047G00090 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.1e-259
Identity = 452/481 (93.97%), Postives = 464/481 (96.47%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQR-PLRLPLLSSGFSTRSSSTGT 60
           MVVSLRLPLISSLLSPASSR  NFAFSRR LGISWR QR PLRLPLLSS FS RSSST T
Sbjct: 1   MVVSLRLPLISSLLSPASSRSPNFAFSRRALGISWRHQRPPLRLPLLSSPFSPRSSSTNT 60

Query: 61  EIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120
           EIR +++KNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ
Sbjct: 61  EIRTESSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 120

Query: 121 VYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180
            YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT
Sbjct: 121 EYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPT 180

Query: 181 SETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240
           SETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA
Sbjct: 181 SETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEA 240

Query: 241 EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300
           EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN
Sbjct: 241 EKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHN 300

Query: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360
           LGQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQVV
Sbjct: 301 LGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVV 360

Query: 361 IIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEI 420
           IIPIWKKDGDKAEVL+AASSVKE LQTAG+KVKLDES+Q+T GWKFNFWEMKGVPLRIEI
Sbjct: 361 IIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMKGVPLRIEI 420

Query: 421 GPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT 480
           GPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLLQQA+TFRDR 
Sbjct: 421 GPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVKETLDEIQSSLLQQARTFRDRR 480

[Close]

BLAST of HG10012295 vs. ExPASy TrEMBL
Match: A0A0A0L6I6 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.7e-256
Identity = 452/507 (89.15%), Postives = 470/507 (92.70%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQR---PLRLPLLSSGFSTRSSST 60
           MVVSLRLPLISSLLSPASSR SN AFSRR LGISWR QR   PLR PLLSS FS RSSST
Sbjct: 1   MVVSLRLPLISSLLSPASSRSSNLAFSRRALGISWRHQRQRPPLRFPLLSSPFSPRSSST 60

Query: 61  GTEIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120
            TEI +D++KNRV EG ITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA
Sbjct: 61  NTEIHIDSSKNRVSEGPITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEA 120

Query: 121 IQVYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180
           IQ YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR
Sbjct: 121 IQEYLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVR 180

Query: 181 PTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240
           PTSETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE
Sbjct: 181 PTSETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPE 240

Query: 241 EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300
           EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS
Sbjct: 241 EAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTS 300

Query: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360
           HNLGQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQ
Sbjct: 301 HNLGQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQ 360

Query: 361 VVIIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRI 420
           VVIIPIWKKDGDKAEVL+AASSVKE LQTAG+KVKLDES+Q+T GWKFNFWEMKGVPLRI
Sbjct: 361 VVIIPIWKKDGDKAEVLNAASSVKEFLQTAGLKVKLDESDQKTAGWKFNFWEMKGVPLRI 420

Query: 421 EIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRD 480
           EIGPKDVAS+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK  LDEIQSSLLQQA+TFRD
Sbjct: 421 EIGPKDVASKSVVISRRDIPGKQGKVFGISMEPSVLEAYVKERLDEIQSSLLQQARTFRD 480

Query: 481 RT-------KKNKQSVKQKKSIREIWN 498
                     + K+++ + K  R  W+
Sbjct: 481 SNIVDVSSYNELKEAISEGKWARGPWS 507

[Close]

BLAST of HG10012295 vs. ExPASy TrEMBL
Match: A0A6J1G067 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.2e-255
Identity = 445/504 (88.29%), Postives = 469/504 (93.06%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLP +SSLLSPAS     F FSRR LGISWRQQRP RLPLL+ G S RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISWRQQRPFRLPLLAPGLSVRSSSTGTE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDN+KNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPP+LAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           +PIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD+SEQRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 VPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDDSEQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           PKDVA+QSVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLL+QAKTFRD   
Sbjct: 421 PKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDEIQSSLLEQAKTFRDSNV 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ Q K  R  W+
Sbjct: 481 VDVSSYNELKEAISQGKWARGPWS 500

[Close]

BLAST of HG10012295 vs. ExPASy TrEMBL
Match: A0A6J1HTC6 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.7e-253
Identity = 444/504 (88.10%), Postives = 467/504 (92.66%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLP +SSLLSPAS     F FSRR LGIS RQQRP RLPLL+  FS RSSSTGTE
Sbjct: 1   MVVSLRLPSLSSLLSPASP----FTFSRRALGISLRQQRPFRLPLLAPRFSVRSSSTGTE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           I VDN+KNRVPEGAITPRSQDFNAWYLD+IANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IHVDNSKNRVPEGAITPRSQDFNAWYLDIIANAELADYGPVRGTMVIRPYGYAIWEAIQE 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLN KFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHS+RDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE
Sbjct: 181 ETIVNHMFTQWIHSHRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAAIPVIAGRKSK+ETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAIPVIAGRKSKMETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQFTDENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPP+LAPIQVVI
Sbjct: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPRLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           IPIWKK+G KAEVL+AA+SV+ +L+TAGIKVKLD SEQRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 IPIWKKEGVKAEVLNAAASVEVNLRTAGIKVKLDNSEQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           PKDVA+QSVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLL+QAKTFRD   
Sbjct: 421 PKDVANQSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDEIQSSLLEQAKTFRDSNV 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ Q K  R  W+
Sbjct: 481 VDVSSYNELKEAISQGKWARGPWS 500

[Close]

BLAST of HG10012295 vs. ExPASy TrEMBL
Match: A0A6J1C8A3 (Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.2e-246
Identity = 431/504 (85.52%), Postives = 460/504 (91.27%), Query Frame = 0


Query: 1   MVVSLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGTE 60
           MVVSLRLP +S+L SPAS+  ++  F RR       QQR   LP L++G+S +SSST TE
Sbjct: 1   MVVSLRLPSLSALFSPASTTTTSTTFFRRAPVTLRPQQRLFHLPRLAAGYSAQSSSTETE 60

Query: 61  IRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQV 120
           IR DN+KNRVPEGAITPRS+DFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQ 
Sbjct: 61  IRADNSKNRVPEGAITPRSKDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQA 120

Query: 121 YLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180
           YLN KFKETGH+NMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS
Sbjct: 121 YLNVKFKETGHDNMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTS 180

Query: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAE 240
           ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHA+PEEAE
Sbjct: 181 ETIVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHANPEEAE 240

Query: 241 KEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300
           KEAIQMIDIYTKF+YEQAA+PVI GRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL
Sbjct: 241 KEAIQMIDIYTKFAYEQAAMPVIPGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNL 300

Query: 301 GQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360
           GQNFSRAFGTQF DENGQRQHVWQTSWA+STRFVGGIIMTHGDDGGLMLPPKLAPIQVVI
Sbjct: 301 GQNFSRAFGTQFADENGQRQHVWQTSWAISTRFVGGIIMTHGDDGGLMLPPKLAPIQVVI 360

Query: 361 IPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIG 420
           IPIWKKD D+AEVLSA+SSVKESLQTAGIKVKLD+S+QRTPGWKFNFWEMKGVPLRIEIG
Sbjct: 361 IPIWKKDDDRAEVLSASSSVKESLQTAGIKVKLDDSDQRTPGWKFNFWEMKGVPLRIEIG 420

Query: 421 PKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRDRT- 480
           P+DV+S+SVVISRRDIPGKQGKVFGISMEPS+LEAYVK TLDEIQSSLLQQAKTFRD   
Sbjct: 421 PRDVSSRSVVISRRDIPGKQGKVFGISMEPSILEAYVKDTLDEIQSSLLQQAKTFRDSNI 480

Query: 481 ------KKNKQSVKQKKSIREIWN 498
                  + K+++ Q K  R  W+
Sbjct: 481 VDVSSYNELKEAISQGKWARGPWS 504

[Close]

BLAST of HG10012295 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 764.6 bits (1973), Expect = 5.1e-221
Identity = 381/475 (80.21%), Postives = 421/475 (88.63%), Query Frame = 0


Query: 4   SLRLPLISSLLSPASSRPSNFAFSRRTLGISWRQQRPLRLPLLSSGFSTRSSSTGT-EIR 63
           SLRLP ++SLL PA++R    A  RRT+ +   + RPL      SGF+T  S T + E +
Sbjct: 5   SLRLPSLTSLLFPATTRYP--ATLRRTVCL---RNRPL------SGFATAPSGTASPETK 64

Query: 64  VDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGYAIWEAIQVYL 123
                    + A+TPRSQDFNAWYLDVIA+AELADYGPVRGTMVIRPYGYAIWEAIQ YL
Sbjct: 65  SSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTMVIRPYGYAIWEAIQDYL 124

Query: 124 NAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEEKLVVRPTSET 183
           N KFKETGH NMYFPQFIPYSFIEKEASHVEGFSPELALVT+GGGKELEEKLVVRPTSET
Sbjct: 125 NVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSET 184

Query: 184 IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFVRTLEFLWQEGHTAHASPEEAEKE 243
           IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPF+RTLEFLWQEGHTAHA+PEEAEKE
Sbjct: 185 IVNHMFTQWIHSYRDLPLMINQWANVTRWEMRTKPFIRTLEFLWQEGHTAHATPEEAEKE 244

Query: 244 AIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDRKALQAGTSHNLGQ 303
           A QMI+IYT+F++EQ AIPVI GRKSK+ETFAGAD TYTIEAMMGDRKALQAGTSHNLGQ
Sbjct: 245 AKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEAMMGDRKALQAGTSHNLGQ 304

Query: 304 NFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLPPKLAPIQVVIIP 363
           NFSRAFGTQF DENG+RQHVWQTSWAVSTRFVGGIIMTHGDD GLMLPPK+APIQVVI+P
Sbjct: 305 NFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGDDTGLMLPPKIAPIQVVIVP 364

Query: 364 IWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEMKGVPLRIEIGPK 423
           IWKKD +K  VLSAASSVKE+LQTAG++VKLD+++QRTPGWKFNFWEMKG+PLRIEIGP+
Sbjct: 365 IWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGWKFNFWEMKGIPLRIEIGPR 424

Query: 424 DVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQQAKTFRD 478
           DV+S SVV+SRRD+PGK GKVFGISMEPS L AYVK  LDEIQ+SLL++A +FRD
Sbjct: 425 DVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDEIQTSLLEKALSFRD 468

[Close]

BLAST of HG10012295 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.9e-97
Identity = 186/465 (40.00%), Postives = 287/465 (61.72%), Query Frame = 0


Query: 53  RSSSTGTEIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGY 112
           ++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+  
Sbjct: 31  KASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWSM 90

Query: 113 AIWEAIQVYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEE 172
           AIWE +Q++ +A+ K+   +N YFP F+    +EKE  H+EGF+PE+A VT  G  +LE 
Sbjct: 91  AIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEV 150

Query: 173 KLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFVRTLEFLWQEGHT 232
            + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PF+R+ EFLWQEGHT
Sbjct: 151 PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHT 210

Query: 233 AHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDR-K 292
           A A+  EA++E +Q++++Y +   E  A+PV+ G KS+ E FAG   T ++EA + +  +
Sbjct: 211 AFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGR 270

Query: 293 ALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLP 352
            +Q  TSH LGQNF++ F   F +E  + + VWQ SWA STR +G +IMTHGDD GL+LP
Sbjct: 271 GVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLP 330

Query: 353 PKLAPIQVVIIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEM 412
           PK+A +QVV+IP+  KD +   +  A ++   +L  AGI+ + D  +  +PGWK++ WEM
Sbjct: 331 PKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEM 390

Query: 413 KGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQ 472
           KGVPLRIEIGP+D+ +  V   RRD   K+    G     SL+E +VK  L++IQ ++ +
Sbjct: 391 KGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRG-----SLVE-HVKELLEKIQQNMYE 450

Query: 473 QAKTFRDRTKKNK-------QSVKQKKSIREIWNLECSISYEKKA 509
            AK  R+   +         +++ +KK I   W  E  +  + KA
Sbjct: 451 VAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKA 486

[Close]

BLAST of HG10012295 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.9e-97
Identity = 186/465 (40.00%), Postives = 287/465 (61.72%), Query Frame = 0


Query: 53  RSSSTGTEIRVDNTKNRVPEGAITPRSQDFNAWYLDVIANAELADYGPVRGTMVIRPYGY 112
           ++SS+G + +  + K     G    + ++F  WY +V    ++ +Y  + G  ++RP+  
Sbjct: 31  KASSSGQKKK--DVKKETGLGLSVKKDENFGEWYSEV-CKQDMIEYYDISGCYILRPWSM 90

Query: 113 AIWEAIQVYLNAKFKETGHENMYFPQFIPYSFIEKEASHVEGFSPELALVTIGGGKELEE 172
           AIWE +Q++ +A+ K+   +N YFP F+    +EKE  H+EGF+PE+A VT  G  +LE 
Sbjct: 91  AIWEIMQIFFDAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEV 150

Query: 173 KLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFVRTLEFLWQEGHT 232
            + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PF+R+ EFLWQEGHT
Sbjct: 151 PIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHT 210

Query: 233 AHASPEEAEKEAIQMIDIYTKFSYEQAAIPVIAGRKSKVETFAGADKTYTIEAMMGDR-K 292
           A A+  EA++E +Q++++Y +   E  A+PV+ G KS+ E FAG   T ++EA + +  +
Sbjct: 211 AFATKAEADEEVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGR 270

Query: 293 ALQAGTSHNLGQNFSRAFGTQFTDENGQRQHVWQTSWAVSTRFVGGIIMTHGDDGGLMLP 352
            +Q  TSH LGQNF++ F   F +E  + + VWQ SWA STR +G +IMTHGDD GL+LP
Sbjct: 271 GVQGATSHCLGQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLP 330

Query: 353 PKLAPIQVVIIPIWKKDGDKAEVLSAASSVKESLQTAGIKVKLDESEQRTPGWKFNFWEM 412
           PK+A +QVV+IP+  KD +   +  A ++   +L  AGI+ + D  +  +PGWK++ WEM
Sbjct: 331 PKVASVQVVVIPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEM 390

Query: 413 KGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYVKGTLDEIQSSLLQ 472
           KGVPLRIEIGP+D+ +  V   RRD   K+    G     SL+E +VK  L++IQ ++ +
Sbjct: 391 KGVPLRIEIGPRDLENDQVRTVRRDNGVKEDIPRG-----SLVE-HVKELLEKIQQNMYE 450

Query: 473 QAKTFRDRTKKNK-------QSVKQKKSIREIWNLECSISYEKKA 509
            AK  R+   +         +++ +KK I   W  E  +  + KA
Sbjct: 451 VAKQKREACVQEVKTWDEFIKALNEKKLILAPWCDEEEVERDVKA 486

[Close]

BLAST of HG10012295 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-19
Identity = 65/166 (39.16%), Postives = 93/166 (56.02%), Query Frame = 0


Query: 339 MTHGDDGGLMLPPKLAPIQVVIIPI-WKKDGDKAEVLSAASSVKESLQTAGIKVKLDESE 398
           MTHGDD GL+ PPK+AP+QVV+I +  K   D  E+  A  +V+ +L  AGI+ + D  +
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 399 QRTPGWKFNFWEMKGVPLRIEIGPKDVASQSVVISRRDIPGKQGKVFGISMEPSLLEAYV 458
             + GWK+   E+ GVPLRIE GP+D+A+  V I  RD   K     G  +E       V
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIE------QV 208

Query: 459 KGTLDEIQSSLLQQAK-TFRDRTKKNK------QSVKQKKSIREIW 497
           K  L++IQS+L   AK    + T+K +      +++ QKK I   W
Sbjct: 209 KDLLEKIQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPW 248

The following BLAST results are available for this feature:

BLAST of HG10012295 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878181.1	1.4e-268	93.06	proline--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida]	[more]
KAA0039089.1	4.4e-259	93.97	proline--tRNA ligase [Cucumis melo var. makuwa] >TYK14878.1 proline--tRNA ligase...	[more]
XP_004149956.3	3.5e-256	89.15	proline--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >XP_03173867...	[more]
XP_023541480.1	2.3e-255	88.69	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_022945172.1	8.6e-255	88.29	proline--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]

BLAST of HG10012295 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYR6	7.2e-220	80.21	Proline--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q5SM28	7.1e-135	54.93	Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)...	[more]
Q72GF9	3.5e-134	54.71	Proline--tRNA ligase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / H...	[more]
B3QPX3	1.5e-132	57.96	Proline--tRNA ligase OS=Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) OX=51...	[more]
B3EP23	1.5e-132	55.29	Proline--tRNA ligase OS=Chlorobium phaeobacteroides (strain BS1) OX=331678 GN=pr...	[more]

BLAST of HG10012295 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CUH7	2.1e-259	93.97	Prolyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A0A0L6I6	1.7e-256	89.15	Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G213510 PE=3 SV=1	[more]
A0A6J1G067	4.2e-255	88.29	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111449493 PE=3 SV=1	[more]
A0A6J1HTC6	6.7e-253	88.10	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111467631 PE=3 SV=1	[more]
A0A6J1C8A3	1.2e-246	85.52	Prolyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111008350 PE=3 SV=1	[more]

BLAST of HG10012295 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52520.1	5.1e-221	80.21	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.1	7.9e-97	40.00	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	7.9e-97	40.00	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	1.7e-19	39.16	tRNA synthetase-related / tRNA ligase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002316	Proline-tRNA ligase, class IIa	PRINTS	PR01046	TRNASYNTHPRO	coord: 133..151 score: 26.32 coord: 213..224 score: 34.26 coord: 174..185 score: 41.67 coord: 204..212 score: 46.09
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	TIGRFAM	TIGR00408	TIGR00408	coord: 76..498 e-value: 5.4E-154 score: 511.6
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 2..485
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	HAMAP	MF_01571	Pro_tRNA_synth_type3	coord: 75..517 score: 38.918007
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 357..436 e-value: 1.1E-16 score: 60.7
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 70..350 e-value: 2.3E-105 score: 354.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..64
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..74
None	No IPR available	PANTHER	PTHR43382:SF7	BNAC09G28510D PROTEIN	coord: 2..485
None	No IPR available	CDD	cd00862	ProRS_anticodon_zinc	coord: 346..507 e-value: 7.61868E-62 score: 199.446
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 81..344
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 346..477
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 351..478 e-value: 1.3E-34 score: 120.5
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 169..338 e-value: 5.6E-20 score: 72.0
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 102..350 score: 23.655905
IPR033721	Prolyl-tRNA synthetase, catalytic domain	CDD	cd00778	ProRS_core_arch_euk	coord: 81..340 e-value: 1.10729E-169 score: 477.858

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10012295.1	HG10012295.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009553	embryo sac development
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0010109	regulation of photosynthesis
biological_process	GO:0048316	seed development
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding