HG10012147 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10012147
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGlucan endo-1,3-beta-D-glucosidase
LocationChr01: 18098454 .. 18101128 (-)
RNA-Seq ExpressionHG10012147
SyntenyHG10012147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTCCCTTTCTTTCTCTACTCTTATCATTCTTTTTCCTCTCACTCTCAAGTAAACCTCAATCCCCTACTTCTTCTTCTTCTCCATTTCTGTATTTCTCTCTAACTTCCTCCTCCTTCCTTTCTTGCAGGTGCAGAACTCTCCAGTCTCGTCGGGATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCCCCGTCCGATTCCGTCAGTTTAATCAAATCCCTAAACGCCCAAATCGTCAAAATCTACGACACTAATCCCCAAATTCTCAAATCCCTAAAAAACACTGATCTACGAGTCTCCGTTATGGTCCCTAACGACCTCATCATCAACATTTCTTCTAACCAGAATCTCGCCGACGAATGGGTTCGGACAAATGTCTTCCCGTTTTACCCCCAAACCAAAATCCGTTACCTCCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAACGAAACCTGGTTCAGTTTAGTGCCGGCGATGCGCCGAATCAAACGCTCTTTGAAAACTTACGGGATTCGTAAAGTGAAAGTTGGAACGTCCTCCGCCATGGATGTTCTTCAATCGTCTTTTCCGCCGTCGAACGGGACGTTCCGAGCGGATATTGCCGATCGAGTTATGAAACCCATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTTGATGCGTATCCGTATTTCCCTTGGGCTTTGGATTCCGTCAACATCAAACTAGATTACGCTCTGTTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTAATCTACACGAATCTCTTCGATCAAATGGTGGATTCGGTAATTTTCGCTATGAAGCGTCTCGGGTTTCCCGATATCCGAGTTTTTATCGCCGAAACCGGTTGGCCTAACGGCGGCGATTACGACCAAATCGGTGCCAACATTCACAACGCCGCCGTGTACAACCGGAATATCGTCAAACGGGTCACTTCCAAGCCGCCGGTTGGTACACCGGCTCGACCGGGCCGAATCCTGCCGACGATTATATTCTCATTGTATAATGAGAATCAGAAACCGGGTCCGGGCACGGAAAGGCATTTCGGACTGTTGTATCCGAACCGGAAGGCGGTGTATGAGATAGATTTGAGTGGGTCGACGCCGGAGACGAAGTTTAAGCCGCTGCCGGCGGCGGAAACCAATGAGCCGTACAAGGGAAAGATTTGGTGCATAGTGGGGAAGGGGGCGAATATGAGTGATGTTGTTGGGGCTTTATCATACGCTTGCTCGCAGGGGAATAAAACCTGTGAACCGATCCGGAAGGGCGGTCCATGTTATGAACCGGATTCTTTAAAACGACACGCGAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTCAATGGCTTAGCAACTCAAACAATCAAAGACCCAAGTAAGTTATTTTTATTATTATTTTTATAATTTTTAAAATTTAAACTCCGATCTCACACGATAAAAATAAATATGTCAATTATCGTTAAATTAAAAAGACTATTATTTTTTAAAATTTAAATTCCAATGGTATATGTTAAATACTGCACTTAAAAATTGATTTTATAATATAAATGAGAATTATTTTGAAACAGTTAAAATTATTTTTGTTATTTTTAAAATTAGTAAAAAATTTATTTTAATTATGATAATAGAGATTTTAATCACGTCAAAATCACTCTCAACCATTTTACTTATTTTTTACGCCATTCTTATTTTTACTTTAATTCTTCTAAATTTCTTCATTCTATCATTAATGTTTGAAAAGTTATTAAGTTTCTATATAAAAAATGTAAAAGTTTGGTGTGTCATTAAATAGTGGTTTTATTAACATATTTAGATATGGTTGCACACCAATTTAATGAATTTTGTTGATGAGTTAGATTTTCTGTCGAGTAGCATTCAATTGCTATATTCTCATGTTCAAATAAAATAATAATTTAATGACATGAATGGTTTTTATGAACCATATATGTAAAATCAATTTTTTTTTTAGTATAATAAATATTGTATAATGAAGATTCGAGTCTTTGAACTCAAGAAAAAAAGTACATATAAGTTTATGATATTTAATATATTTAGCCTATATATCATTTCCTTTGCATTTAAATAATTTTTAAAAGTAGAAAAATAATAAAAAAAAAAATATTTACACAAAATAGTAAAATTTGTAGGTAGTTTTGATAGAATAAAAGTCTACCAGGGTCTATAAGGGATAAAAATGATAAAAGTCTATTACTTAATAGAAGTCTATCAATGTCTAATAGTATTTTTTTTGCTATTTTCTGTAAATTGTTTGACATTTTTTCTATTGGTAAAAATTTCTCTTTGAAAAAGTATGCATTTAAATAATTAATGTTGTTCCAAATTTATTATTTCTAATATTATCGCTAAAAAATTTAGTCAAATAATATATTATATTGTACGAAGATTTAAATCTACTATAGTAAAATTATTAGGCTGTGTTGATATAGTATCTTTGAGATTAGGTTTAAATGACTAACTAGAGCTTAACTCAATTAATTAAGTAGTTTGTTATAAATTAAAAAAATTAAAGATTTTAATTTCTCACAAAAAAAAAAAACATTTATATTTTGCAGGTTATGGAAAATGCAAGTTCCCGAGCGTGACGTTGCAAGGATAA

mRNA sequence

ATGGCGATTCCCTTTCTTTCTCTACTCTTATCATTCTTTTTCCTCTCACTCTCAAGTGCAGAACTCTCCAGTCTCGTCGGGATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCCCCGTCCGATTCCGTCAGTTTAATCAAATCCCTAAACGCCCAAATCGTCAAAATCTACGACACTAATCCCCAAATTCTCAAATCCCTAAAAAACACTGATCTACGAGTCTCCGTTATGGTCCCTAACGACCTCATCATCAACATTTCTTCTAACCAGAATCTCGCCGACGAATGGGTTCGGACAAATGTCTTCCCGTTTTACCCCCAAACCAAAATCCGTTACCTCCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAACGAAACCTGGTTCAGTTTAGTGCCGGCGATGCGCCGAATCAAACGCTCTTTGAAAACTTACGGGATTCGTAAAGTGAAAGTTGGAACGTCCTCCGCCATGGATGTTCTTCAATCGTCTTTTCCGCCGTCGAACGGGACGTTCCGAGCGGATATTGCCGATCGAGTTATGAAACCCATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTTGATGCGTATCCGTATTTCCCTTGGGCTTTGGATTCCGTCAACATCAAACTAGATTACGCTCTGTTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTAATCTACACGAATCTCTTCGATCAAATGGTGGATTCGGTAATTTTCGCTATGAAGCGTCTCGGGTTTCCCGATATCCGAGTTTTTATCGCCGAAACCGGTTGGCCTAACGGCGGCGATTACGACCAAATCGGTGCCAACATTCACAACGCCGCCGTGTACAACCGGAATATCGTCAAACGGGTCACTTCCAAGCCGCCGGTTGGTACACCGGCTCGACCGGGCCGAATCCTGCCGACGATTATATTCTCATTGTATAATGAGAATCAGAAACCGGGTCCGGGCACGGAAAGGCATTTCGGACTGTTGTATCCGAACCGGAAGGCGGTGTATGAGATAGATTTGAGTGGGTCGACGCCGGAGACGAAGTTTAAGCCGCTGCCGGCGGCGGAAACCAATGAGCCGTACAAGGGAAAGATTTGGTGCATAGTGGGGAAGGGGGCGAATATGAGTGATGTTGTTGGGGCTTTATCATACGCTTGCTCGCAGGGGAATAAAACCTGTGAACCGATCCGGAAGGGCGGTCCATGTTATGAACCGGATTCTTTAAAACGACACGCGAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTCAATGGCTTAGCAACTCAAACAATCAAAGACCCAAGTTATGGAAAATGCAAGTTCCCGAGCGTGACGTTGCAAGGATAA

Coding sequence (CDS)

ATGGCGATTCCCTTTCTTTCTCTACTCTTATCATTCTTTTTCCTCTCACTCTCAAGTGCAGAACTCTCCAGTCTCGTCGGGATCAACTACGGCCAGCTCGGCAACAACCTCCCTCCCCCGTCCGATTCCGTCAGTTTAATCAAATCCCTAAACGCCCAAATCGTCAAAATCTACGACACTAATCCCCAAATTCTCAAATCCCTAAAAAACACTGATCTACGAGTCTCCGTTATGGTCCCTAACGACCTCATCATCAACATTTCTTCTAACCAGAATCTCGCCGACGAATGGGTTCGGACAAATGTCTTCCCGTTTTACCCCCAAACCAAAATCCGTTACCTCCTCGTCGGAAACGAAATCATCTCCTCCACTGGAAACGAAACCTGGTTCAGTTTAGTGCCGGCGATGCGCCGAATCAAACGCTCTTTGAAAACTTACGGGATTCGTAAAGTGAAAGTTGGAACGTCCTCCGCCATGGATGTTCTTCAATCGTCTTTTCCGCCGTCGAACGGGACGTTCCGAGCGGATATTGCCGATCGAGTTATGAAACCCATGTTGCAGTTTCTGAACCGGACGAAATCGTTCTTCTTCCTTGATGCGTATCCGTATTTCCCTTGGGCTTTGGATTCCGTCAACATCAAACTAGATTACGCTCTGTTTGAATCGAAGAACATCACGTATTCGGATCCGGGTTCGGGTTTAATCTACACGAATCTCTTCGATCAAATGGTGGATTCGGTAATTTTCGCTATGAAGCGTCTCGGGTTTCCCGATATCCGAGTTTTTATCGCCGAAACCGGTTGGCCTAACGGCGGCGATTACGACCAAATCGGTGCCAACATTCACAACGCCGCCGTGTACAACCGGAATATCGTCAAACGGGTCACTTCCAAGCCGCCGGTTGGTACACCGGCTCGACCGGGCCGAATCCTGCCGACGATTATATTCTCATTGTATAATGAGAATCAGAAACCGGGTCCGGGCACGGAAAGGCATTTCGGACTGTTGTATCCGAACCGGAAGGCGGTGTATGAGATAGATTTGAGTGGGTCGACGCCGGAGACGAAGTTTAAGCCGCTGCCGGCGGCGGAAACCAATGAGCCGTACAAGGGAAAGATTTGGTGCATAGTGGGGAAGGGGGCGAATATGAGTGATGTTGTTGGGGCTTTATCATACGCTTGCTCGCAGGGGAATAAAACCTGTGAACCGATCCGGAAGGGCGGTCCATGTTATGAACCGGATTCTTTAAAACGACACGCGAGTTTTGCGTTTAGTTCGTACTGGGCCCAGTTTAGAAAGGTTGGTGGGACCTGTTACTTCAATGGCTTAGCAACTCAAACAATCAAAGACCCAAGTTATGGAAAATGCAAGTTCCCGAGCGTGACGTTGCAAGGATAA

Protein sequence

MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Homology
BLAST of HG10012147 vs. NCBI nr
Match: XP_038886980.1 (probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida])

HSP 1 Score: 922.2 bits (2382), Expect = 1.8e-264
Identity = 446/465 (95.91%), Postives = 459/465 (98.71%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MAIPFLSLLL FFFLSLSSAE+SSLVGINYGQLGNNLPPPSDSV+LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFLSLLLPFFFLSLSSAEISSLVGINYGQLGNNLPPPSDSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILK+LKNTDLRVSVMVPNDLIINISSNQNLAD+WVRTNV PFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKTLKNTDLRVSVMVPNDLIINISSNQNLADDWVRTNVRPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           +SSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIA+R
Sbjct: 121 VSSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIAER 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VMKPMLQFLNRTKSFFFLD YPYFPWA+DSVNIKLDYALFESKNITYSDPGSGLIYTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPYFPWAIDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPD+RVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDVRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNENQKPGPGTERHFGLLYPNR AVYE+DLSGSTPET+FKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENQKPGPGTERHFGLLYPNRTAVYEMDLSGSTPETEFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PAAETNEPYKGKIWC+VGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PAAETNEPYKGKIWCVVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSV LQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVVLQG 465

BLAST of HG10012147 vs. NCBI nr
Match: XP_004144397.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypothetical protein Csa_001975 [Cucumis sativus])

HSP 1 Score: 883.6 bits (2282), Expect = 7.2e-253
Identity = 428/464 (92.24%), Postives = 444/464 (95.69%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           M IPFLSLL   FFLSLSSAE+S+LVGINYGQLGNNLPPPS SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILK+LKNTDLRVSVMVPN+LIINISS+QNLAD+WVRTN+ PFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGT SAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VM+PMLQFLNRTKSFFFLD YPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNENQKPGPGTERHFGLLYP  KAVYE+DLSGS  ETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+VGKG NMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of HG10012147 vs. NCBI nr
Match: XP_008460404.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA0068092.1 putative glucan endo-1,3-beta-glucosidase A6 [Cucumis melo var. makuwa])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 425/465 (91.40%), Postives = 445/465 (95.70%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           M IPFLSLLL  FFLSLSSAE+SSLVGINYGQLGNNLP PS SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQIL +LKNTDLRVSVMVPN+LIINISSNQNLAD+WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGTSSAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VM+PMLQFLNRTKSF+FLD YPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP+ KAVYE+DLSGST ETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AETNEPYKG+IWC+VGKG NMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. NCBI nr
Match: XP_022931078.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata])

HSP 1 Score: 865.1 bits (2234), Expect = 2.7e-247
Identity = 415/465 (89.25%), Postives = 440/465 (94.62%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MAIPF SLLL+F+FLSL+SA++S+LVGINYGQLGNNLPPP DSV LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILKSL N+D++VSVMVPNDLIINISSNQNLAD WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TW SLVPAMRRIKRSLKTYGI KVKVGTSSAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VMKPMLQFLNRTKSFFFLD YP+FPWA D VNIKLDYALFESKNITYSDPGSGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYPNR AVYE+DLSG TPE  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PAAETNEPYKGKIWC+V +GAN S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. NCBI nr
Match: XP_023532592.1 (probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864.0 bits (2231), Expect = 5.9e-247
Identity = 415/465 (89.25%), Postives = 439/465 (94.41%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MAIPF SLLL+F+FLSL+SA++S+LVGINYGQLGNNLPPP DSV LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILKSL N+D++VSVMVPNDLIINISSNQNLAD WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TW SLVPAMRRIKRSLK YGI KVKVGTSSAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VMKPMLQFLNRTKSFFFLD YP+FPWA D VNIKLDYALFESKNITYSDPGSGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYPNR AVYE+DLSG TPE  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PAAETNEPYKGKIWC+V +GAN S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 4.5e-149
Identity = 241/443 (54.40%), Postives = 327/443 (73.81%), Query Frame = 0

Query: 21  ELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVP 80
           +L+S +GINYG+ GNNLP P  S++ IKS+ A  VK+YD +P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGNETWFSLVPAMRRIK 140
           N  I  +SSNQ +ADEWVRTN+ P+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 RSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFLDA 200
            SL+ +GI  +KVGT  AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+ 
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPDIR 260
           +PYF W+ + +N  LD+ALF+  + TY+DP +GL+Y NL DQM+DSV+FAM +LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSLYN 320
           + I+ETGWPN GD D+ GANI NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAAETNEPYKGKIWCIVGKG 380
           ENQK G GT+RH+G+L+P+   +Y++D +G TP T F PLP    N PYKG++WC+  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 ANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
           AN +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

BLAST of HG10012147 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-92
Identity = 172/433 (39.72%), Postives = 255/433 (58.89%), Query Frame = 0

Query: 26  VGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVPNDLII 85
           VG+ YG+  ++LP PS  V LI+  N + V+IYD N Q+LK+  NT + + + VPN  + 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 86  NISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGNETWFSLVPAMRRIKRSLKT 145
             S +Q+  D W++ +V P+YP TKI Y+ VG E            +VPAM+ +  +L+ 
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTALRK 145

Query: 146 YGI-RKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFLDAYPYF 205
            G+ R++KV T+ ++ +L  SFPPS G F +  A   ++PML+FL   KS F +D YPY+
Sbjct: 146 VGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYA-YFLRPMLEFLAENKSPFMIDLYPYY 205

Query: 206 PWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPDIRVFIA 265
            +     N+ LDY LFES +    DP +GL+Y N+FD  VD++ +A+  L F  I++ + 
Sbjct: 206 AYRDSPNNVSLDYVLFESSS-EVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMVT 265

Query: 266 ETGWP-NGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSLYNENQ 325
           ETGWP  G   ++  A+  NA  YN NI++ V +    GTPA+PG  +   IFSL+NEN+
Sbjct: 266 ETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQ--GTPAKPGEAMNVYIFSLFNENR 325

Query: 326 KPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAAETNEPYKGKIWCIVGKGANM 385
           K G  +ER++GL YP++ +VY++D +G +         ++ TN       WCI    A+ 
Sbjct: 326 KAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSN---SSGTNSSGSSNSWCIASSKASE 385

Query: 386 SDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGL 445
            D+ GAL +AC  GN  C  I+   PC++PD+L  HASF F+SY+ Q R     C F G 
Sbjct: 386 RDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGA 445

Query: 446 ATQTIKDPSYGKC 457
             +  KDPSY KC
Sbjct: 446 GVKVNKDPSYDKC 451

BLAST of HG10012147 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 165/458 (36.03%), Postives = 264/458 (57.64%), Query Frame = 0

Query: 22  LSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVPN 81
           ++S +GI YG+  +NLP P+    LI+ LN + V+IYD N  +LK+  NT + + + VPN
Sbjct: 23  MASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPN 82

Query: 82  DLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGNETWFSLVPAMRRIKR 141
             ++  +  Q+  D W+  N+ P+YP TKI  + VG E+  +  N T   ++PAMR I  
Sbjct: 83  ADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGL-VLPAMRNIHT 142

Query: 142 SLKTYGI-RKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFLDA 201
           +LK  G+ +K+K+ +S ++ +L  SFPPS+ +F +      +KPML+FL   +S F +D 
Sbjct: 143 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASF-SKKHSAFLKPMLEFLVENESPFMIDL 202

Query: 202 YPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPDIR 261
           YPY+ +   +  + L+YALFES +    DP +GL+Y+N+FD  +D++ FA+  + F  ++
Sbjct: 203 YPYYAYRDSTEKVPLEYALFESSS-QVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVK 262

Query: 262 VFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSLYN 321
           V + E+GWP+ G   +  A   NA  YN N+++ V   P  GTPA+PG  +   +FSL+N
Sbjct: 263 VMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP--GTPAKPGEEIDVYLFSLFN 322

Query: 322 ENQKPGPGTERHFGLLYPNRKAVYEIDL--------------SGSTPETKFKPLPAAETN 381
           EN+KPG  +ER++G+ Y N   VY +D               +G++P     P+    + 
Sbjct: 323 ENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNST 382

Query: 382 EPYKG------KIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 441
               G      K WCI    A+++++  AL +AC  GN  C  ++   PC+EPD++  HA
Sbjct: 383 VTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHA 442

Query: 442 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKF 459
           S+AF++Y+ Q       C FNG + +  KDPSYG C +
Sbjct: 443 SYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of HG10012147 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.9e-87
Identity = 172/457 (37.64%), Postives = 263/457 (57.55%), Query Frame = 0

Query: 7   SLLLSFFFL--SLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQI 66
           SLL   F L  +  SA   S +G+NYGQL +NLP    +V+L+KS   Q V+++   P +
Sbjct: 9   SLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAV 68

Query: 67  LKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISST 126
           +K+  NT + + +   N  I  ++SN N+A ++V++NV  FYP + I  + VGNE+++S 
Sbjct: 69  IKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSG 128

Query: 127 GNETWFSLVPAMRRIKRSLKTYGI-RKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMK 186
             +    L+PAM+ ++ +L    +  KVKV T  AM VL  S+PPS+G F   + D  MK
Sbjct: 129 DQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD-TMK 188

Query: 187 PMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQM 246
            +LQF +   + F +  YPYF +        L + LF+  N    D G+G  YTN+FD  
Sbjct: 189 ALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQ-PNAGQVDSGNGHKYTNMFDAQ 248

Query: 247 VDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGT 306
           VD+V  A+  +GF DI + +AETGWP+GGD +++G ++ NA  Y  N++  + SK  VGT
Sbjct: 249 VDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSK--VGT 308

Query: 307 PARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAA 366
           P  PG+ + T +FSLY+E++K G  +E++FGL  P+    Y++   G    T+    PA 
Sbjct: 309 PLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDV---GLLKNTQNPTTPAT 368

Query: 367 ETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFA 426
            T  P     WC+   G +   + G ++YAC QG   C PI+ GG C+EP+++K HA++ 
Sbjct: 369 PTPTPKAAGSWCVPKPGVSDDQLTGNINYACGQG-IDCGPIQPGGACFEPNTVKAHAAYV 428

Query: 427 FSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
            + Y+    +    C F+  AT T  +PSYG C FPS
Sbjct: 429 MNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPS 457

BLAST of HG10012147 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 4.2e-86
Identity = 173/464 (37.28%), Postives = 277/464 (59.70%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           ++I FL + LS F     S+     +G+NYGQ+ +NLPPPS++V L++S + Q V++Y  
Sbjct: 5   ISIYFLLIFLSHF----PSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGA 64

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           +P I+K+L  T + + +   N  + +++S+ N A +W+ +NV PFYP +KI  + VGNEI
Sbjct: 65  DPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEI 124

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGI-RKVKVGTSSAMDVLQSSFPPSNGTFRADIAD 180
           + S        L+PAM+ ++++L+   +  K+KV T ++M VL SS PPS+G+F A    
Sbjct: 125 LMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGY-Q 184

Query: 181 RVMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNL 240
             +K +LQFL+ T S F ++ YP+F +  D     L + LFE  N    D  +G+ YTN+
Sbjct: 185 TGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFE-PNAGRVDSKTGIKYTNM 244

Query: 241 FDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKP 300
           FD  VD+V  A+K +GF  + + +AETGW + GD +++GA++ NA  YN N++  + S  
Sbjct: 245 FDAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSM- 304

Query: 301 PVGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKP 360
            VGTP  PG+ + T IF+LY+EN KPGP +ER FGL   +   VY++ L+ S        
Sbjct: 305 -VGTPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKS-------- 364

Query: 361 LPAAETNEPYKGKI----WCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDS 420
              + +++   GK+    WC+  KGA   ++  +L +AC  G   C  I+ GG C+EP++
Sbjct: 365 ---SSSSQTPSGKVTSSGWCVPKKGATNEELQASLDWACGHG-IDCGAIQPGGACFEPNN 424

Query: 421 LKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFP 460
           +  HA++A + Y+ +  K    C F+  AT T ++PSY  C +P
Sbjct: 425 VVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of HG10012147 vs. ExPASy TrEMBL
Match: A0A0A0LBR1 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.5e-253
Identity = 428/464 (92.24%), Postives = 444/464 (95.69%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           M IPFLSLL   FFLSLSSAE+S+LVGINYGQLGNNLPPPS SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLFFLFFLSLSSAEISTLVGINYGQLGNNLPPPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILK+LKNTDLRVSVMVPN+LIINISS+QNLAD+WVRTN+ PFYPQTKIRYLLVGNEI
Sbjct: 61  NPQILKALKNTDLRVSVMVPNELIINISSSQNLADQWVRTNILPFYPQTKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGT SAMDVLQSSFPPSNGTFRADIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTPSAMDVLQSSFPPSNGTFRADIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VM+PMLQFLNRTKSFFFLD YPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFFFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGA+IHNAAVYNRNIVKRVTSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGASIHNAAVYNRNIVKRVTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNENQKPGPGTERHFGLLYP  KAVYE+DLSGS  ETKFKPL
Sbjct: 301 VGTPARPGRMLPTIIFSLYNENQKPGPGTERHFGLLYPKGKAVYEMDLSGSRAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AE NEPYKGKIWC+VGKG NMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAERNEPYKGKIWCVVGKGVNMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 465
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQ 464

BLAST of HG10012147 vs. ExPASy TrEMBL
Match: A0A5A7VIC4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00460 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.6e-252
Identity = 425/465 (91.40%), Postives = 445/465 (95.70%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           M IPFLSLLL  FFLSLSSAE+SSLVGINYGQLGNNLP PS SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQIL +LKNTDLRVSVMVPN+LIINISSNQNLAD+WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGTSSAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VM+PMLQFLNRTKSF+FLD YPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP+ KAVYE+DLSGST ETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AETNEPYKG+IWC+VGKG NMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. ExPASy TrEMBL
Match: A0A1S3CCE3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.6e-252
Identity = 425/465 (91.40%), Postives = 445/465 (95.70%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           M IPFLSLLL  FFLSLSSAE+SSLVGINYGQLGNNLP PS SV+LIKSLNAQIVKIYD 
Sbjct: 1   MPIPFLSLLLFPFFLSLSSAEISSLVGINYGQLGNNLPSPSHSVNLIKSLNAQIVKIYDA 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQIL +LKNTDLRVSVMVPN+LIINISSNQNLAD+WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILNALKNTDLRVSVMVPNELIINISSNQNLADQWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TWFSLVPAMRRIK SLKTYGI KVKVGTSSAMDVLQSSFPPSNGTFR DIADR
Sbjct: 121 ISSTGNQTWFSLVPAMRRIKHSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFREDIADR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VM+PMLQFLNRTKSF+FLD YPYFPW+ DSVNIKLDYALFESKNITYSDP SGL+YTNLF
Sbjct: 181 VMRPMLQFLNRTKSFYFLDVYPYFPWSSDSVNIKLDYALFESKNITYSDPVSGLVYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKR+TSKPP
Sbjct: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRMTSKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPGR+LPTIIFSLYNEN+K GPGTERHFGLLYP+ KAVYE+DLSGST ETKFKPL
Sbjct: 301 VGTPARPGRVLPTIIFSLYNENEKGGPGTERHFGLLYPSGKAVYEMDLSGSTAETKFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           P AETNEPYKG+IWC+VGKG NMS+VVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA
Sbjct: 361 PEAETNEPYKGRIWCVVGKGVNMSEVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRK+GGTCYFNGLATQTIKDPSYGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKIGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. ExPASy TrEMBL
Match: A0A6J1EXC8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 415/465 (89.25%), Postives = 440/465 (94.62%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MAIPF SLLL+F+FLSL+SA++S+LVGINYGQLGNNLPPP DSV LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILKSL N+D++VSVMVPNDLIINISSNQNLAD WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TW SLVPAMRRIKRSLKTYGI KVKVGTSSAMDVLQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKTYGIHKVKVGTSSAMDVLQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VMKPMLQFLNRTKSFFFLD YP+FPWA D VNIKLDYALFESKNITYSDPGSGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIR+FIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRIFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYPNR AVYE+DLSG TPE  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PAAETNEPYKGKIWC+V +GAN S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPYKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDP+YGKCKFPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPNYGKCKFPSVTLQG 465

BLAST of HG10012147 vs. ExPASy TrEMBL
Match: A0A6J1K875 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.7e-245
Identity = 411/465 (88.39%), Postives = 438/465 (94.19%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MAIPF SLLL+F+FLSL+SA++S+LVGINYGQLGNNLPPP DSV LIKSLNAQIVKIYD 
Sbjct: 1   MAIPFSSLLLAFYFLSLTSAKISTLVGINYGQLGNNLPPPPDSVDLIKSLNAQIVKIYDV 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           NPQILKSL N+D++VSVMVPNDLIINISSNQNLAD WVRTN+ PFYPQ KIRYLLVGNEI
Sbjct: 61  NPQILKSLNNSDIKVSVMVPNDLIINISSNQNLADHWVRTNILPFYPQIKIRYLLVGNEI 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADR 180
           ISSTGN+TW SLVPAMRRIKRSLK YGI KVKVG+SSAMD+LQSSFPPSNGTFRADI+DR
Sbjct: 121 ISSTGNQTWISLVPAMRRIKRSLKIYGIHKVKVGSSSAMDILQSSFPPSNGTFRADISDR 180

Query: 181 VMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLF 240
           VMKPMLQFLNRTKSFFFLD YP+FPWA D VNIKLDYALFESKNITYSDPGSGL YTNLF
Sbjct: 181 VMKPMLQFLNRTKSFFFLDVYPFFPWASDPVNIKLDYALFESKNITYSDPGSGLTYTNLF 240

Query: 241 DQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPP 300
           DQMVDSVIFAMKRLG+PDIRVFI+ETGWPNGGDYDQIGANIHNAAVYNRNIVKRVT+KPP
Sbjct: 241 DQMVDSVIFAMKRLGYPDIRVFISETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTAKPP 300

Query: 301 VGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPL 360
           VGTPARPG +LPTIIFSLYNENQKPG GTERHFGLLYPNR AVYE+DLSG TPE  FKPL
Sbjct: 301 VGTPARPGWVLPTIIFSLYNENQKPGLGTERHFGLLYPNRSAVYEMDLSGKTPEADFKPL 360

Query: 361 PAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHA 420
           PAAETNEP+KGKIWC+V +GAN S+V GAL++AC QGNKTCEPI+KGGPCYEPDSL RHA
Sbjct: 361 PAAETNEPFKGKIWCMVSRGANKSEVAGALAWACLQGNKTCEPIQKGGPCYEPDSLIRHA 420

Query: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTLQG 466
           SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKC FPSVTLQG
Sbjct: 421 SFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCMFPSVTLQG 465

BLAST of HG10012147 vs. TAIR 10
Match: AT3G07320.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 662.5 bits (1708), Expect = 2.4e-190
Identity = 315/462 (68.18%), Postives = 381/462 (82.47%), Query Frame = 0

Query: 6   LSLLLSFFFLSL----SSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTN 65
           +SLLL  F LSL    S A+ S   GINYGQLGNNLP PSDSV+LIKSLNA+ VK+YD N
Sbjct: 1   MSLLLHLFALSLLISVSGAKFSGRPGINYGQLGNNLPSPSDSVNLIKSLNAKRVKLYDAN 60

Query: 66  PQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEII 125
           P+IL +L  TD+ VSVMVPN+L++NIS + +L+D+W+R+N+ PFYP TKIRYLLVGNEI+
Sbjct: 61  PKILAALNGTDITVSVMVPNELLVNISKSASLSDDWIRSNILPFYPTTKIRYLLVGNEIL 120

Query: 126 SSTGNETWFSLVPAMRRIKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADRV 185
           S   +E   SLVPAMR+I+RSLK+ G++KVKVGT+ A DVLQSSFPPS+G FR DI+  +
Sbjct: 121 SLPDSELKSSLVPAMRKIQRSLKSLGVKKVKVGTTLATDVLQSSFPPSSGEFREDISGLI 180

Query: 186 MKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFD 245
           MKPMLQFLNRTKSF F+D YPYF WA D  ++ LDYA+FES N+T +DP S L Y NLFD
Sbjct: 181 MKPMLQFLNRTKSFLFVDVYPYFAWAQDPTHVDLDYAIFESTNVTVTDPVSNLTYHNLFD 240

Query: 246 QMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPV 305
           QM+D+ +FAMKR+G+PDIR+++AETGWPN GDYDQIGANI+NAA YNRN+VK++ + PPV
Sbjct: 241 QMIDAFVFAMKRVGYPDIRIWVAETGWPNNGDYDQIGANIYNAATYNRNVVKKLAADPPV 300

Query: 306 GTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLP 365
           GTPARPG++LP  +F+LYNENQK GPGTERHFGLL+PN   VY IDLSG T E K + LP
Sbjct: 301 GTPARPGKVLPAFVFALYNENQKTGPGTERHFGLLHPNGTQVYGIDLSGKT-EYK-ESLP 360

Query: 366 AAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHAS 425
           A E N+ YKGKIWC+V KGAN + +  ALSYACSQGN TC+PI++GGPC +PD    HAS
Sbjct: 361 APENNDLYKGKIWCVVAKGANWTQLGDALSYACSQGNNTCDPIQRGGPCQKPDLTVLHAS 420

Query: 426 FAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPSVTL 464
           +AFSSYWAQFRK+GGTC FNGLATQTIKDPSYG+C+FPSVTL
Sbjct: 421 YAFSSYWAQFRKIGGTCSFNGLATQTIKDPSYGRCEFPSVTL 460

BLAST of HG10012147 vs. TAIR 10
Match: AT3G23770.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-153
Identity = 253/445 (56.85%), Postives = 332/445 (74.61%), Query Frame = 0

Query: 22  LSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVPN 81
           L+S +GINYG+LGNNLP P  S++LIK++ A  VK+YD +P+ LK L  T+L V++MVPN
Sbjct: 33  LASRIGINYGKLGNNLPFPYQSINLIKTIKAGHVKLYDADPETLKLLSTTNLYVTIMVPN 92

Query: 82  DLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGN---ETWFSLVPAMRR 141
           + II+I ++Q  AD WV TNV PF+PQT+IR++LVGNE++S + +   + W +LVPAMR+
Sbjct: 93  NQIISIGADQAAADNWVATNVLPFHPQTRIRFVLVGNEVLSYSSDQDKQIWANLVPAMRK 152

Query: 142 IKRSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFL 201
           +  SL+  GI  +KVGT  AMD L+SSFPPS+GTFR DIA  VM P+L+FLN T SFFFL
Sbjct: 153 VVNSLRARGIHNIKVGTPLAMDALRSSFPPSSGTFREDIAVPVMLPLLKFLNGTNSFFFL 212

Query: 202 DAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPD 261
           D YPYFPW+ D VN  LD+ALFES N TY+DP +GL+YTNL DQM+DSVIFAM +LG+P+
Sbjct: 213 DVYPYFPWSTDPVNNHLDFALFES-NSTYTDPQTGLVYTNLLDQMLDSVIFAMTKLGYPN 272

Query: 262 IRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSL 321
           I + I+ETGWPN GD  + GANI NAA YNRN++K++T+ PP+GTPAR G  +PT +FSL
Sbjct: 273 ISLAISETGWPNDGDIHETGANIVNAATYNRNLIKKMTANPPLGTPARRGAPIPTFLFSL 332

Query: 322 YNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAAETNEPYKGKIWCIVG 381
           +NENQKPG GTERH+G+L P+   +Y+ID SG    + F  LP    N P+KG +WC+  
Sbjct: 333 FNENQKPGSGTERHWGILNPDGTPIYDIDFSGRRSFSGFDSLPKPSNNVPFKGNVWCVAV 392

Query: 382 KGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTC 441
            GA+ +++  AL++AC + N TC  +  GG CY P ++  HAS+AFSSYWAQFR     C
Sbjct: 393 DGADEAELGQALNFACGRSNATCAALAPGGECYAPVTVTWHASYAFSSYWAQFRNQSSQC 452

Query: 442 YFNGLATQTIKDPSYGKCKFPSVTL 464
           YFNGLA +T  +P   +CKFPSVTL
Sbjct: 453 YFNGLARETTTNPGNERCKFPSVTL 476

BLAST of HG10012147 vs. TAIR 10
Match: AT4G14080.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.2e-150
Identity = 241/443 (54.40%), Postives = 327/443 (73.81%), Query Frame = 0

Query: 21  ELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQILKSLKNTDLRVSVMVP 80
           +L+S +GINYG+ GNNLP P  S++ IKS+ A  VK+YD +P+ L  L  T+L V++ VP
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISSTGNETWFSLVPAMRRIK 140
           N  I  +SSNQ +ADEWVRTN+ P+YPQT+IR++LVGNEI+S        +LVPAMR+I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 RSLKTYGIRKVKVGTSSAMDVLQSSFPPSNGTFRADIADRVMKPMLQFLNRTKSFFFLDA 200
            SL+ +GI  +KVGT  AMD L+SSFPPSNGTFR +I   VM P+L+FLN T S+FFL+ 
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQMVDSVIFAMKRLGFPDIR 260
           +PYF W+ + +N  LD+ALF+  + TY+DP +GL+Y NL DQM+DSV+FAM +LG+P +R
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHS-TYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMR 276

Query: 261 VFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGTPARPGRILPTIIFSLYN 320
           + I+ETGWPN GD D+ GANI NAA YNRN++K++++ PP+GTP+RPG  +PT +FSL+N
Sbjct: 277 LAISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFN 336

Query: 321 ENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKPLPAAETNEPYKGKIWCIVGKG 380
           ENQK G GT+RH+G+L+P+   +Y++D +G TP T F PLP    N PYKG++WC+  +G
Sbjct: 337 ENQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEG 396

Query: 381 ANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDSLKRHASFAFSSYWAQFRKVGGTCYF 440
           AN +++   L  AC+Q N TC  +  G  CYEP S+  HAS+A +SYWAQFR     C+F
Sbjct: 397 ANETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFF 456

Query: 441 NGLATQTIKDPSYGKCKFPSVTL 464
           NGLA +T  +P   +CKFPSVTL
Sbjct: 457 NGLAHETTTNPGNDRCKFPSVTL 478

BLAST of HG10012147 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-102
Identity = 189/465 (40.65%), Postives = 286/465 (61.51%), Query Frame = 0

Query: 1   MAIPFLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDT 60
           MA+ FL   L   FLS   A  S +VG+NYG++ NNLP P   V+L+KS     +KI+DT
Sbjct: 1   MAVSFLPYFLILSFLSAIDAH-SGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDT 60

Query: 61  NPQILKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEI 120
           +  +L +L N+ ++V V +PN+L+ + +S+Q+ AD W++T++ P++P T+I  + VGNE+
Sbjct: 61  DKNVLTALANSKIKVIVALPNELLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEV 120

Query: 121 ISSTGNETWFSLVPAMRRIKRSLKTYGIRK-VKVGTSSAMDVLQSSFPPSNGTFRADIAD 180
                   +  LV AM+ I  SL  Y + K +K+ +  A+  L +S+PPS+G+F+ ++ +
Sbjct: 121 FVDPTITPY--LVNAMKNIHTSLVKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIE 180

Query: 181 RVMKPMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNL 240
            V+KPML  L +T S+  ++AYP+F +A ++  I LDYALF+ +N    D G+GL Y +L
Sbjct: 181 PVVKPMLALLQQTSSYLMVNAYPFFAYAANADKISLDYALFK-ENAGNIDSGTGLKYNSL 240

Query: 241 FDQMVDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKP 300
           FD  +D+V  A+  +GF  ++V + ETGWP+ GD ++IGA+  NAA YN  +VKRV +  
Sbjct: 241 FDAQIDAVYAALSAVGFKGVKVMVTETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGK 300

Query: 301 PVGTPARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLSGSTPETKFKP 360
             GTP RP   L   +F+L+NENQKPGP +ER++GL YPN   VY +      P TK   
Sbjct: 301 --GTPLRPTEPLNVYLFALFNENQKPGPTSERNYGLFYPNEGKVYNV------PFTKKST 360

Query: 361 LPA----AETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGPCYEPDS 420
            P      +    ++G  WC+         +  AL YAC +G   C PI+ G  CY P+S
Sbjct: 361 TPVNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPES 420

Query: 421 LKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           L+ HAS+AF+SY+ +  +  GTC+F G A    + P YGKC+FP+
Sbjct: 421 LEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQPPRYGKCEFPT 453

BLAST of HG10012147 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.2e-100
Identity = 187/471 (39.70%), Postives = 284/471 (60.30%), Query Frame = 0

Query: 5   FLSLLLSFFFLSLSSAELSSLVGINYGQLGNNLPPPSDSVSLIKSLNAQIVKIYDTNPQI 64
           FL LLLS F LS + +     +G+NYG++ + LP     V L+KS     VKI+D +P +
Sbjct: 9   FLLLLLSPFSLSDAGS-----IGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSV 68

Query: 65  LKSLKNTDLRVSVMVPNDLIINISSNQNLADEWVRTNVFPFYPQTKIRYLLVGNEIISST 124
           LK+L  + ++V+V +PN+L+ + +   + A  WV+ NV  ++P T+I  + VGNE+   T
Sbjct: 69  LKALSGSGIKVTVDLPNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDT 128

Query: 125 GNETWFSLVPAMRRIKRSLKTYGIRK-VKVGTSSAMDVLQSSFPPSNGTFRADIADRVMK 184
            N T F L+PAMR I ++L ++ +   +K+ +  A+  LQ+S+P S+G+FR ++ D V+K
Sbjct: 129 HNTTSF-LIPAMRNIHKALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIK 188

Query: 185 PMLQFLNRTKSFFFLDAYPYFPWALDSVNIKLDYALFESKNITYSDPGSGLIYTNLFDQM 244
           PML FL  T S   ++ YP+F +  +S  I LDYAL   +N    D G+GL Y NLFD  
Sbjct: 189 PMLDFLRETGSRLMINVYPFFAYEGNSDVIPLDYALLR-ENPGMVDSGNGLRYFNLFDAQ 248

Query: 245 VDSVIFAMKRLGFPDIRVFIAETGWPNGGDYDQIGANIHNAAVYNRNIVKRVTSKPPVGT 304
           +D+V  AM  L + DI + + ETGWP+ GD +++GA + NAA YN N+++R+ ++   GT
Sbjct: 249 IDAVFAAMSALKYDDIEIIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRG--GT 308

Query: 305 PARPGRILPTIIFSLYNENQKPGPGTERHFGLLYPNRKAVYEIDLS----------GSTP 364
           P RP   L   +F+L+NEN+K GP +ER++GL +P+ K VY+I  +          G TP
Sbjct: 309 PLRPKADLTVYLFALFNENKKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRDGGHTP 368

Query: 365 ET----KFKPLPAAETNEPYKGKIWCIVGKGANMSDVVGALSYACSQGNKTCEPIRKGGP 424
            T      KP  +   ++   G  WC+    A    + G L YAC +G   C PI+ G  
Sbjct: 369 VTGGDQVTKPPMSGGVSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGAN 428

Query: 425 CYEPDSLKRHASFAFSSYWAQFRKVGGTCYFNGLATQTIKDPSYGKCKFPS 461
           CY PD+L+ HASFAF+SY+ +  + GG+CYF G A    + P YG+C+FP+
Sbjct: 429 CYSPDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPPKYGRCEFPT 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886980.11.8e-26495.91probable glucan endo-1,3-beta-glucosidase A6 [Benincasa hispida][more]
XP_004144397.17.2e-25392.24probable glucan endo-1,3-beta-glucosidase A6 [Cucumis sativus] >KGN58384.1 hypot... [more]
XP_008460404.11.4e-25191.40PREDICTED: probable glucan endo-1,3-beta-glucosidase A6 [Cucumis melo] >KAA00680... [more]
XP_022931078.12.7e-24789.25probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita moschata][more]
XP_023532592.15.9e-24789.25probable glucan endo-1,3-beta-glucosidase A6 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q069154.5e-14954.40Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FJU93.0e-9239.72Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q8VYE51.5e-8836.03Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q94G862.9e-8737.64Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q9M0694.2e-8637.28Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LBR13.5e-25392.24(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G634360 PE=3... [more]
A0A5A7VIC46.6e-25291.40(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3CCE36.6e-25291.40(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103499229 PE=3 SV... [more]
A0A6J1EXC81.3e-24789.25(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437372 P... [more]
A0A6J1K8752.7e-24588.39(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491531 PE=... [more]
Match NameE-valueIdentityDescription
AT3G07320.12.4e-19068.18O-Glycosyl hydrolases family 17 protein [more]
AT3G23770.11.8e-15356.85O-Glycosyl hydrolases family 17 protein [more]
AT4G14080.13.2e-15054.40O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.11.5e-10240.65O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.15.2e-10039.70O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 373..458
e-value: 1.6E-39
score: 147.3
IPR012946X8 domainPFAMPF07983X8coord: 374..445
e-value: 1.5E-17
score: 64.0
NoneNo IPR availableGENE3D1.20.58.1040coord: 369..462
e-value: 2.4E-6
score: 29.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..354
e-value: 2.9E-117
score: 393.2
NoneNo IPR availablePANTHERPTHR32227:SF341BNAC03G35190D PROTEINcoord: 12..456
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..347
e-value: 5.2E-101
score: 338.4
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 259..272
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 12..456
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10012147.1HG10012147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds