HG10011706 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10011706 vs. NCBI nr
Match: XP_004133715.1 (MACPF domain-containing protein At4g24290 [Cucumis sativus] >XP_011650569.1 MACPF domain-containing protein At4g24290 [Cucumis sativus] >KAE8650184.1 hypothetical protein Csa_010735 [Cucumis sativus])

HSP 1 Score: 1055.8 bits (2729), Expect = 1.2e-304
Identity = 536/604 (88.74%), Postives = 540/604 (89.40%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSER YNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
           REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNF Q+SYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSVLGAT VRQAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of HG10011706 vs. NCBI nr
Match: XP_008452249.1 (PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis melo] >KAA0060601.1 MACPF domain-containing protein [Cucumis melo var. makuwa] >TYK02270.1 MACPF domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 531/604 (87.91%), Postives = 538/604 (89.07%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVP+SIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSER YNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNF Q+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSV GAT VRQAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of HG10011706 vs. NCBI nr
Match: XP_038904757.1 (MACPF domain-containing protein At4g24290 [Benincasa hispida] >XP_038904758.1 MACPF domain-containing protein At4g24290 [Benincasa hispida])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.2e-300
Identity = 529/604 (87.58%), Postives = 536/604 (88.74%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAI SIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIESIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSI+CDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIRCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+T+QPSEPSEVQKSLKEMADKRFSE IAQYG GSER Y+NDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTIQPSEPSEVQKSLKEMADKRFSENIAQYGTGSERIYSNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
           REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GN  QES+DRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNVRQESHDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSVLGATAVRQ EWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVLGATAVRQPEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of HG10011706 vs. NCBI nr
Match: XP_023540812.1 (MACPF domain-containing protein At4g24290 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.5 bits (2510), Expect = 3.1e-279
Identity = 502/605 (82.98%), Postives = 514/605 (84.96%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN + SRLIEIDE VGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFNDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TL   EPSEVQK LK+MADKRF+ET+ QYGMGSE  YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS++FIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PK FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDP 420

Query: 421 NGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLR 480
           N N  QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NSNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480

Query: 481 FSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGP 540
           F SVLGAT V+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 547
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEE 600

BLAST of HG10011706 vs. NCBI nr
Match: KAG7031360.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 969.5 bits (2505), Expect = 1.2e-278
Identity = 502/605 (82.98%), Postives = 513/605 (84.79%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDE VGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVL DHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TL   EPSEVQK LK+MADKRF+ET+ QYGMGSE  YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS++FIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PK FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDP 420

Query: 421 NGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLR 480
           N N  QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NSNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480

Query: 481 FSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGP 540
           F SVLGAT V+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 547
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEE 600

BLAST of HG10011706 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-217
Identity = 388/601 (64.56%), Postives = 453/601 (75.37%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I E   + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQM------------------------------- 120
            I NV KSIKCDKGER RFRSD+L FQQM                               
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 ---------------------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
                                      +L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIE 240
           +KMGGKDVIY KQQHS+ LQ   P ++QK LKE+ADKRF E    +  GSER   + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIP 300
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISM+FIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTE 420
            ASLQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSS PKI+QL +
Sbjct: 361 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 420

Query: 421 DPNGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLR 480
           D N +  QES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V + G +NVLFLR
Sbjct: 421 DLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLR 480

Query: 481 LRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPG 540
           L FS V+GAT V+ +EW+ + G A KSGLISTLISHHF+   KPPP+PADVNINSA+YPG
Sbjct: 481 LCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPG 540

BLAST of HG10011706 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 287/604 (47.52%), Postives = 367/604 (60.76%), Query Frame = 0

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQM----------------------------------------- 129
           CDKGERTR RSD+LSF QM                                         
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 -----------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
                             L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSTTLQPSEPSEVQKSLKEMADKRF------SETIAQYGMGSERNYNNDKIEIREQ 249
           +KQ   +     EP E+QK LK   D+RF      S++ A    G  +  N  +  ++  
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 253

Query: 250 HLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITSLLN 309
                 A    +  ++I+ +C RRGG D +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 254 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 313

Query: 310 GVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQF 369
           G+PG+GF+SHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  SLQF
Sbjct: 314 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 373

Query: 370 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPNGNF 429
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 374 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 433

Query: 430 HQESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNQGMRNVLFLRL 489
            +E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL
Sbjct: 434 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 493

Query: 490 RFSSVLGATAV-RQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPP---KP-ADVNINSA 540
            FS  L A+AV R++ W+     + KSG+ S +IS   ST   P P   KP + ++INSA
Sbjct: 494 GFS--LDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSA 553

BLAST of HG10011706 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 5.0e-107
Identity = 235/620 (37.90%), Postives = 339/620 (54.68%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDECVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMV--------------------------------------- 131
            CDKGERTRFRSD+L F +M                                        
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 ---------------------LCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
                                L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSET--IAQYGMGSERNYNNDKIEIREQH 251
           V+ ++Q  S+ L   +   ++  L ++ D+ F+ +  ++   +    ++++ + +  E  
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFGDASS------AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPI 311
             F D  +      +I+    I  IC +RGG  R +  H EWL TV  +PD I+  FIPI
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGR-AKSHSEWLITVPDKPDAINFNFIPI 309

Query: 312 TSLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 371
           TSLL  VPGSG +SHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNM 369

Query: 372 AS----LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIF- 431
           AS    L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL ++     
Sbjct: 370 ASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVG 429

Query: 432 -QLTEDPNGNFHQESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSI 491
            ++T++       +  D  RY E +  K FSHVCT PV+ D +                I
Sbjct: 430 EKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFI 489

Query: 492 VTGAQLQVVNQGMRNVLFLRLRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLI------ 534
           VTGAQL+V   G ++VL LRLR++ V     V+ +   G  G + KSG+ S++       
Sbjct: 490 VTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSG 549

BLAST of HG10011706 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.9e-101
Identity = 241/602 (40.03%), Postives = 318/602 (52.82%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+E    ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQM--------------------------------------------- 133
                R  V SF +M                                             
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 -------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
                        VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEIREQHLRFGDASSA 253
            S+   P   SE++  + +M   RF E  +Q   G                L++ D    
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD---- 263

Query: 254 ISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITSLLNGVPGSGFMSH 313
               KDI  I +RRGG D +   H  W +TV   PD+I+MTF PI SLL GVPG   ++ 
Sbjct: 264 ----KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTR 323

Query: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVN 373
           AI LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++
Sbjct: 324 AIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFIS 383

Query: 374 TTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPNGNFH--------- 433
              V VGR+PVTGLRL LEG + N LSIHLQHL S PKI Q    P+ + H         
Sbjct: 384 ADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQ----PHWDSHVPIGAPKWQ 443

Query: 434 -QESYDRRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNQGMRNVLFL 493
             E  D R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L
Sbjct: 444 GPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHL 503

Query: 494 RLRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYP 534
           +L FS V G T +R++ W+ +P   A SG +                            P
Sbjct: 504 KLLFSKVPGCT-IRRSVWDHTP--VASSGRLE---------------------------P 559

BLAST of HG10011706 vs. ExPASy TrEMBL
Match: A0A5D3BT29 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00720 PE=4 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 7.4e-303
Identity = 531/604 (87.91%), Postives = 538/604 (89.07%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVP+SIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSER YNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNF Q+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSV GAT VRQAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of HG10011706 vs. ExPASy TrEMBL
Match: A0A1S3BTG2 (MACPF domain-containing protein At4g24290 OS=Cucumis melo OX=3656 GN=LOC103493321 PE=4 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 7.4e-303
Identity = 531/604 (87.91%), Postives = 538/604 (89.07%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGI+
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVP+SIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSER YNNDKIEI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
           REQHLRFGDAS+AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNF Q+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSV GAT VRQAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of HG10011706 vs. ExPASy TrEMBL
Match: A0A0A0L5E1 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G110020 PE=4 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.6e-289
Identity = 515/604 (85.26%), Postives = 519/604 (85.93%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDE VGHEIVLPGGIL
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSER YNND    
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNND---- 240

Query: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITS 300
                            KDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISM+FIPITS
Sbjct: 241 -----------------KDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480
           GNF Q+SYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGPP 540
           SSVLGAT VRQAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSAVYPGGPP
Sbjct: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 547
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 583

BLAST of HG10011706 vs. ExPASy TrEMBL
Match: A0A6J1J8T9 (MACPF domain-containing protein At4g24290 OS=Cucurbita maxima OX=3661 GN=LOC111482322 PE=4 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.3e-278
Identity = 498/605 (82.31%), Postives = 514/605 (84.96%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVL DHVRKAVPSSWDPAALARFIETFGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TL   EPSEVQK LK+MADKRF+ET+ QYGMGSE  YN++K E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPIT 300
           REQHL+F DASSAIS LK DI+ I KRRGGSDRI+LPHHEWLQTVQLEPDVIS++FIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS PK FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDP 420

Query: 421 NGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLR 480
           N N  QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NNNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480

Query: 481 FSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGP 540
           F SVLGAT V+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 547
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+L DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEE 600

BLAST of HG10011706 vs. ExPASy TrEMBL
Match: A0A6J1FQI8 (MACPF domain-containing protein At4g24290 OS=Cucurbita moschata OX=3662 GN=LOC111447540 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.4e-277
Identity = 500/605 (82.64%), Postives = 511/605 (84.46%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGIL 60
           MALKVHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDE VGH IVLPGGI 
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQ---------------------------------- 120
           ISNVPKSIKCDKGERTRFRSDVLSFQ                                  
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ------------------------QMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180
                                   QMVL DHVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEI 240
           KMGGKDVIYMKQQHS+TL   EPSEVQK LK+MADKRF+ET+ QYGM SE  YNNDK E 
Sbjct: 181 KMGGKDVIYMKQQHSSTL---EPSEVQKRLKDMADKRFTETMGQYGMSSEWIYNNDKFES 240

Query: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPIT 300
           REQHL+F DASSAIS LK DIV I KRRGGSDRI+LPHHEWLQTVQLEPDVIS++FIPIT
Sbjct: 241 REQHLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPIT 300

Query: 301 SLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSS P  FQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPISFQLTEDP 420

Query: 421 NGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLR 480
           N N  QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRNVLFLRL 
Sbjct: 421 NSNSGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLF 480

Query: 481 FSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPGGP 540
           F SVLGAT V+QAEWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FCSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 547
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEE 600

BLAST of HG10011706 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 756.5 bits (1952), Expect = 1.5e-218
Identity = 388/601 (64.56%), Postives = 453/601 (75.37%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I E   + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQM------------------------------- 120
            I NV KSIKCDKGER RFRSD+L FQQM                               
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 ---------------------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
                                      +L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIE 240
           +KMGGKDVIY KQQHS+ LQ   P ++QK LKE+ADKRF E    +  GSER   + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIP 300
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISM+FIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300

Query: 301 ITSLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHN 360
           ITSLLNGVPGSGF+SHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ +
Sbjct: 301 ITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQS 360

Query: 361 LASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTE 420
            ASLQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSS PKI+QL +
Sbjct: 361 CASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLED 420

Query: 421 DPNGNFHQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLR 480
           D N +  QES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V + G +NVLFLR
Sbjct: 421 DLNRSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLR 480

Query: 481 LRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYPG 540
           L FS V+GAT V+ +EW+ + G A KSGLISTLISHHF+   KPPP+PADVNINSA+YPG
Sbjct: 481 LCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPG 540

BLAST of HG10011706 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 498.0 bits (1281), Expect = 9.3e-141
Identity = 287/604 (47.52%), Postives = 367/604 (60.76%), Query Frame = 0

Query: 10  AAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGILISNVPKSIK 69
           AAE A+  IG GYD+  D+R   CK    GSRL+EID     +++ PGGI+++NV  SIK
Sbjct: 14  AAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIK 73

Query: 70  CDKGERTRFRSDVLSFQQM----------------------------------------- 129
           CDKGERTR RSD+LSF QM                                         
Sbjct: 74  CDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAY 133

Query: 130 -----------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIY 189
                             L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKDVI+
Sbjct: 134 DGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIH 193

Query: 190 MKQQHSTTLQPSEPSEVQKSLKEMADKRF------SETIAQYGMGSERNYNNDKIEIREQ 249
           +KQ   +     EP E+QK LK   D+RF      S++ A    G  +  N  +  ++  
Sbjct: 194 VKQMRKSN---HEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 253

Query: 250 HLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITSLLN 309
                 A    +  ++I+ +C RRGG D +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 254 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 313

Query: 310 GVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQF 369
           G+PG+GF+SHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  SLQF
Sbjct: 314 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 373

Query: 370 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPNGNF 429
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 374 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 433

Query: 430 HQESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNQGMRNVLFLRL 489
            +E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLFLRL
Sbjct: 434 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 493

Query: 490 RFSSVLGATAV-RQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPP---KP-ADVNINSA 540
            FS  L A+AV R++ W+     + KSG+ S +IS   ST   P P   KP + ++INSA
Sbjct: 494 GFS--LDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSA 553

BLAST of HG10011706 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 389.8 bits (1000), Expect = 3.6e-108
Identity = 235/620 (37.90%), Postives = 339/620 (54.68%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDECVGHEIVLPGGILISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E+ +PG  +  NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMV--------------------------------------- 131
            CDKGERTRFRSD+L F +M                                        
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 ---------------------LCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
                                L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSET--IAQYGMGSERNYNNDKIEIREQH 251
           V+ ++Q  S+ L   +   ++  L ++ D+ F+ +  ++   +    ++++ + +  E  
Sbjct: 190 VVVVRQDKSSDL---DNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFGDASS------AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPI 311
             F D  +      +I+    I  IC +RGG  R +  H EWL TV  +PD I+  FIPI
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGR-AKSHSEWLITVPDKPDAINFNFIPI 309

Query: 312 TSLLNGVPGSGFMSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNL 371
           TSLL  VPGSG +SHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+
Sbjct: 310 TSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNM 369

Query: 372 AS----LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIF- 431
           AS    L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL ++     
Sbjct: 370 ASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVG 429

Query: 432 -QLTEDPNGNFHQESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSI 491
            ++T++       +  D  RY E +  K FSHVCT PV+ D +                I
Sbjct: 430 EKITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFI 489

Query: 492 VTGAQLQVVNQGMRNVLFLRLRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLI------ 534
           VTGAQL+V   G ++VL LRLR++ V     V+ +   G  G + KSG+ S++       
Sbjct: 490 VTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSG 549

BLAST of HG10011706 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 371.3 bits (952), Expect = 1.3e-102
Identity = 241/602 (40.03%), Postives = 318/602 (52.82%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGHEIVLPGGILISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+E    ++ L  G L+ NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQM--------------------------------------------- 133
                R  V SF +M                                             
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 -------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
                        VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIEIREQHLRFGDASSA 253
            S+   P   SE++  + +M   RF E  +Q   G                L++ D    
Sbjct: 204 QSS---PLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD---- 263

Query: 254 ISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIPITSLLNGVPGSGFMSH 313
               KDI  I +RRGG D +   H  W +TV   PD+I+MTF PI SLL GVPG   ++ 
Sbjct: 264 ----KDITVIFRRRGGDD-LEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTR 323

Query: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVN 373
           AI LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++
Sbjct: 324 AIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFIS 383

Query: 374 TTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSSPKIFQLTEDPNGNFH--------- 433
              V VGR+PVTGLRL LEG + N LSIHLQHL S PKI Q    P+ + H         
Sbjct: 384 ADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQ----PHWDSHVPIGAPKWQ 443

Query: 434 -QESYDRRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNQGMRNVLFL 493
             E  D R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L
Sbjct: 444 GPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHL 503

Query: 494 RLRFSSVLGATAVRQAEWEGSPGLAAKSGLISTLISHHFSTVTKPPPKPADVNINSAVYP 534
           +L FS V G T +R++ W+ +P   A SG +                            P
Sbjct: 504 KLLFSKVPGCT-IRRSVWDHTP--VASSGRLE---------------------------P 559

BLAST of HG10011706 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 328.9 bits (842), Expect = 7.5e-90
Identity = 184/338 (54.44%), Postives = 220/338 (65.09%), Query Frame = 0

Query: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDECVGH-EIVLPGGI 60
           MAL++  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I E   + EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  LISNVPKSIKCDKGERTRFRSDVLSFQQM------------------------------- 120
            I NV KSIKCDKGER RFRSD+L FQQM                               
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 ---------------------------VLCDHVRKAVPSSWDPAALARFIETFGTHIIVG 180
                                      +L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSTTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERNYNNDKIE 240
           +KMGGKDVIY KQQHS+ LQ   P ++QK LKE+ADKRF E    +  GSER   + K+E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQ---PEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVE 240

Query: 241 IREQHLRFGDASS--AISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMTFIP 276
            +EQ LRF D SS  + +  +D V +CKRRGG+D  +L H+EWLQTVQ+EPDVISM+FIP
Sbjct: 241 TKEQRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIP 300